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annotate trinity.xml @ 6:a3d60df650b7 draft

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit 46636ba3d63d34e9ee30a2e0ee28cca0213628c8
author iuc
date Wed, 25 May 2016 15:04:27 -0400
parents 8ffafd84a7b8
children 80c6d1ec9cd7
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8ffafd84a7b8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit 72247137f912b62dd5a3bc566524988bce10b515
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1 <tool id="trinity" name="Trinity" version="2.1.1.1">
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2 <description>de novo assembly of RNA-Seq data</description>
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3 <requirements>
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4 <requirement type="package" version="2.1.1">trinity</requirement>
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5 <requirement type="package" version="1.1.2">bowtie</requirement>
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6 <requirement type="package" version="1.2">samtools</requirement>
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7 <requirement type="set_environment">TRINITY_MEM_OPTIONS</requirement>
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8 </requirements>
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9 <command><![CDATA[
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10 Trinity
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12 ## Inputs.
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13 #if $inputs.paired_or_single == "paired":
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15 --left ${ ','.join(['"%s"' % x for x in $inputs.left_input]) }
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17 --right ${ ','.join(['"%s"' % x for x in $inputs.right_input]) }
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19 #if $inputs.left_input[0].is_of_type('fasta'):
0
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20 --seqType fa
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21 #else:
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22 --seqType fq
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23 #end if
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24
0
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25 #if $inputs.strand.is_strand_specific:
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26 --SS_lib_type $inputs.strand.library_type
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27 #end if
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0
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29 $inputs.jaccard_clip
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30
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31 #else:
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32 --single ${ ','.join(['"%s"' % x for x in $inputs.input]) }
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34 #if $inputs.input[0].is_of_type('fasta'):
0
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35 --seqType fa
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36 #else:
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37 --seqType fq
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38 #end if
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39
0
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40 #if $inputs.strand.is_strand_specific:
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41 --SS_lib_type $inputs.strand.library_type
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42 #end if
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43 #end if
3
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44
0
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45 $norm
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46
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47 ## Additional parameters.
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48 #if $additional_params.min_contig_length:
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49 --min_contig_length $additional_params.min_contig_length
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50 #end if
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51 #if $additional_params.long_reads:
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52 --long_reads $additional_params.long_reads
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53 #end if
1
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54 #if $additional_params.guided.is_guided == "yes":
0
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55 --genome_guided_bam $additional_params.guided.genome_guided_bam
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57 #if $additional_params.guided.genome_guided_min_coverage:
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58 --genome_guided_min_coverage $additional_params.guided.genome_guided_min_coverage
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59 #end if
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61 #if $additional_params.guided.genome_guided_min_reads_per_partition:
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62 --genome_guided_min_reads_per_partition $additional_params.guided.genome_guided_min_reads_per_partition
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63 #end if
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64
0
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65 #end if
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66
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67 ## CPU and butterfly options.
2
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68 --CPU \${GALAXY_SLOTS:-4} \${TRINITY_MEM_OPTIONS:---max_memory 1G} --bfly_opts "-V 10 --stderr"
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69
2
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70 ## > $trinity_log 2>&1
0
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71
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72 ]]></command>
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73 <inputs>
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74 <conditional name="inputs">
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75 <param name="paired_or_single" type="select" label="Paired or Single-end data?">
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76 <option value="paired">Paired</option>
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77 <option value="single">Single</option>
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78 </param>
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79 <when value="paired">
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80 <param format="fasta,fastqsanger" name="left_input" multiple="true" type="data" label="Left/Forward strand reads" help=""/>
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81 <param format="fasta,fastqsanger" name="right_input" multiple="true" type="data" label="Right/Reverse strand reads" help=""/>
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82 <conditional name="strand">
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83 <param name="is_strand_specific" type="boolean" checked="false" label="Strand specific data"/>
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84 <when value="false">
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85 </when>
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86 <when value="true">
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87 <param name="library_type" type="select" label="Strand-specific Library Type">
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88 <option value="FR">Forward-Reverse</option>
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89 <option value="RF">Reverse-Forward</option>
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90 </param>
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91 </when>
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92 </conditional>
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93 <param name="jaccard_clip" type="boolean" truevalue="--jaccard_clip" falsevalue="" checked="false" label="Jaccard Clip options" help="set if you expect high gene density with UTR overlap"/>
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94 </when>
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95 <when value="single">
3
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96 <param format="fasta,fastqsanger" name="input" multiple="true" type="data" label="Single-end reads" help=""/>
0
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97 <conditional name="strand">
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98 <param name="is_strand_specific" type="boolean" checked="false" label="Strand specific data"/>
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99 <when value="false">
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100 </when>
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101 <when value="true">
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102 <param name="library_type" type="select" label="Strand-specific Library Type">
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103 <option value="F">F</option>
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104 <option value="R">R</option>
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105 </param>
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106 </when>
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107 </conditional>
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108 </when>
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109 </conditional>
3
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110
0
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111 <param name="norm" type="boolean" truevalue="--normalize_reads" falsevalue="" checked="true" label="Run in silico normalization of reads" help="Defaults to max. read coverage of 50."/>
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112
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113 <section name="additional_params" title="Additional Options" expanded="False">
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114 <param name="min_contig_length" type="integer" optional="true" value="200" min="1" label="Minimum Contig Length" help="All contigs shorter than this will be discarded"/>
3
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115
0
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116 <conditional name="guided">
1
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117 <param name="is_guided" type="select" label="Use the genome guided mode?" help="If you already mapped the reads to the genome, Trinity can use this information">
0
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118 <option value="no">No</option>
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119 <option value="yes">Yes</option>
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120 </param>
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121 <when value="no">
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122 </when>
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123 <when value="yes">
1
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124 <param format="bam" name="genome_guided_bam" type="data" label="Coordinate-sorted BAM file" />
0
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125 <param name="genome_guided_min_coverage" type="integer" optional="true" value="1" min="1" label="Minimum read coverage for identifying an expressed region of the genome"/>
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126 <param name="genome_guided_min_reads_per_partition" type="integer" optional="true" value="10" min="1" label="Minimum number of reads per partition"/>
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127 </when>
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128 </conditional>
3
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129
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130
0
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131 <param format="fasta" name="long_reads" type="data" optional="true" label="Error-corrected or circular consensus (CCS) pac bio reads" help="Experimental feature! Long reads must be in the same orientation as short reads if they are strand specific"/>
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132 </section>
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133 </inputs>
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134 <outputs>
2
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135 <!--data format="txt" name="trinity_log" label="${tool.name} on ${on_string}: log" /-->
0
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136 <data format="fasta" name="assembled_transcripts" label="${tool.name} on ${on_string}: Assembled Transcripts" from_work_dir="trinity_out_dir/Trinity.fasta"/>
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137 </outputs>
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138 <tests>
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139 <test>
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140 <param name="paired_or_single" value="paired"/>
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141 <param name="left_input" value="reads.left.fq" ftype="fastqsanger"/>
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142 <param name="right_input" value="reads.right.fq" ftype="fastqsanger"/>
0
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143 <param name="is_strand_specific" value="true"/>
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144 <param name="norm" value="false"/>
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145 <param name="library_type" value="RF"/>
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146 <output name="assembled_transcripts" file="raw/Trinity.fasta" compare="sim_size" delta="500" />
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147 </test>
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148 <test>
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149 <param name="paired_or_single" value="paired"/>
3
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150 <param name="left_input" value="reads.left.fq" ftype="fastqsanger"/>
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151 <param name="right_input" value="reads.right.fq" ftype="fastqsanger"/>
0
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152 <param name="is_strand_specific" value="true"/>
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153 <param name="norm" value="true"/>
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154 <param name="library_type" value="RF"/>
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155 <output name="assembled_transcripts" file="norm/Trinity.fasta" compare="sim_size" delta="500" />
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156 </test>
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157 </tests>
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158 <help>
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159 Trinity_ assembles transcript sequences from Illumina RNA-Seq data.
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160
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161 .. _Trinity: http://trinityrnaseq.github.io
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162 </help>
3
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163
0
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164 <citations>
6
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165 <citation type="doi">10.1038/nbt.1883</citation>
0
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166 </citations>
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167 </tool>
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168