Mercurial > repos > iuc > snpeff

annotate snpEff_create_db.xml @ 30:0c8a8cd9dd5b draft

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit 4bc3ef84687aa6a39f79c2497a5800a9718825cd
author iuc
date Sat, 28 Sep 2024 16:27:42 +0000
parents 030fe29d4c47
children b7029a54f73e
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0c8a8cd9dd5b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit 4bc3ef84687aa6a39f79c2497a5800a9718825cd
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1 <tool id="snpEff_build_gb" name="SnpEff build:" version="@WRAPPER_VERSION@.galaxy6" profile="22.01">
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2 <description> database from Genbank or GFF record</description>
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3 <macros>
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4 <import>snpEff_macros.xml</import>
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5 </macros>
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6 <requirements>
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7 <expand macro="requirement" />
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8 <requirement type="package" version="1.79">biopython</requirement>
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9 </requirements>
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10 <expand macro="stdio" />
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11 <expand macro="version_command" />
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12 <command><![CDATA[
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13 #if str($input_type.input_type_selector) == "gb" and str($input_type.fasta) == "yes":
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14 python3 '$__tool_directory__/gbk2fa.py' '${input_type.input}' '${output_fasta}' ${input_type.remove_version} &&
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15 #end if
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17 mkdir -p '${snpeff_output.files_path}/${genome_version}' &&
0c8a8cd9dd5b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit 4bc3ef84687aa6a39f79c2497a5800a9718825cd
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18 mkdir -p snpeff_output/'${genome_version}' &&
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20 #if str($input_type.input_type_selector) == "gb":
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21 #if $input_type.input.is_of_type("genbank"):
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22 ln -s '${input_type.input}' 'snpeff_output/${genome_version}/genes.gbk' &&
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23 #elif $input_type.input.is_of_type("genbank.gz"):
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24 ln -s '${input_type.input}' 'snpeff_output/${genome_version}/genes.gbk.gz' &&
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25 #end if
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26 #else:
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27 #if $input_type.reference_source.reference_source_selector == "history":
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28 #if $input_type.reference_source.input_fasta.is_of_type("fasta"):
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29 ln -s '${input_type.reference_source.input_fasta}' 'snpeff_output/${genome_version}/sequences.fa' &&
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30 #elif $input_type.reference_source.input_fasta.is_of_type("fasta.gz"):
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31 ln -s '${input_type.reference_source.input_fasta}' 'snpeff_output/${genome_version}/sequences.fa.gz' &&
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32 #end if
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33 #elif $input_type.reference_source.reference_source_selector == "cached":
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34 ln -s '${input_type.reference_source.ref_file.fields.path}' 'snpeff_output/${genome_version}/sequences.fa' &&
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35 #end if
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36 ln -s '${input_type.input}' 'snpeff_output/${genome_version}/genes.${input_type.input_type_selector}' &&
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37 #end if
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38
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39 snpEff @JAVA_OPTIONS@ build -v
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40 -configOption '${genome_version}'.genome='${genome_version}'
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41 -configOption '${genome_version}'.codonTable='${codon_table}'
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42 #if str($input_type.input_type_selector) == "gb":
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43 -genbank
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44 #elif str($input_type.input_type_selector) == "gff":
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45 -gff3
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46 #elif str($input_type.input_type_selector) == "gtf":
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47 -gtf22
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48 #end if
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49 -dataDir "\$(pwd)/snpeff_output" '${genome_version}' &&
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50 mv snpeff_output/'${genome_version}'/*.bin '${snpeff_output.files_path}/${genome_version}' &&
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51 echo '${genome_version}.genome : ${genome_version}' >> '${snpeff_output.files_path}'/snpEff.config &&
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52 echo '${genome_version}.codonTable : ${codon_table}' >> '${snpeff_output.files_path}'/snpEff.config
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53 ]]></command>
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54 <inputs>
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55 <param name="genome_version" type="text" value="" label="Name for the database" help="For E. coli K12, for example, you may want to use 'EcK12'. Note: Spaces are not allowed in the name and will get converted to underscores.">
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56 <sanitizer>
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57 <valid initial="string.ascii_letters,string.digits">
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58 <add value="_" />
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59 <add value="." />
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60 <add value="-" />
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61 </valid>
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62 <mapping>
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63 <add source=" " target="_" />
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64 </mapping>
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65 </sanitizer>
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66 <validator type="empty_field" message="A genome version name is required" />
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67 </param>
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68 <conditional name="input_type">
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69 <param name="input_type_selector" type="select" display="radio" label="Input annotations are in" help="Specify format for annotations you are using to create SnpEff database">
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70 <option value="gb" selected="true">GenBank</option>
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71 <option value="gff">GFF</option>
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72 <option value="gtf">GTF</option>
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73 </param>
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74 <when value="gb">
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75 <param name="input" type="data" format="genbank,genbank.gz" label="Genbank dataset to build database from" help="This Genbank file will be used to generate snpEff database"/>
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76 <param name="fasta" type="select" display="radio" label="Parse Genbank into Fasta" help="This will generate an additional dataset containing all sequences from Genbank file in FASTA format">
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77 <option value="yes" selected="true">Yes</option>
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78 <option value="no">No</option>
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79 </param>
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80 <param argument="--remove_version" type="boolean" truevalue="--remove_version" falsevalue="" checked="true" label="Remove sequence version label?" help="Genbank sequences have version numbers such as B000564.2. This option removes them leaving only B000564" />
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81 </when>
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82 <when value="gff">
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83 <param name="input" type="data" format="gff3" label="GFF dataset to build database from" help="This GFF file will be used to generate snpEff database"/>
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84 <expand macro="ref_select" />
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85 </when>
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86 <when value="gtf">
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87 <param name="input" type="data" format="gtf" label="GTF dataset to build database from" help="This GTF file will be used to generate snpEff database"/>
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88 <expand macro="ref_select" />
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89 </when>
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90 </conditional>
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91 <param name="codon_table" type="select" label="Select genetic code for this sequence" help="If this sequence uses non-standard genetic code, select one from these options">
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92 <option selected="true" value="Standard">Standard</option>
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93 <option value="Vertebrate_Mitochondrial">Vertebrate_Mitochondrial</option>
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94 <option value="Yeast_Mitochondrial">Yeast_Mitochondrial</option>
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95 <option value="Mold_Mitochondrial">Mold_Mitochondrial</option>
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96 <option value="Protozoan_Mitochondrial">Protozoan_Mitochondrial</option>
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97 <option value="Coelenterate">Coelenterate</option>
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98 <option value="Mitochondrial">Mitochondrial</option>
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99 <option value="Mycoplasma">Mycoplasma</option>
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100 <option value="Spiroplasma">Spiroplasma</option>
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101 <option value="Invertebrate_Mitochondrial">Invertebrate_Mitochondrial</option>
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102 <option value="Ciliate_Nuclear">Ciliate_Nuclear</option>
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103 <option value="Dasycladacean_Nuclear">Dasycladacean_Nuclear</option>
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104 <option value="Hexamita_Nuclear">Hexamita_Nuclear</option>
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105 <option value="Echinoderm_Mitochondrial">Echinoderm_Mitochondrial</option>
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106 <option value="Flatworm_Mitochondrial">Flatworm_Mitochondrial</option>
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107 <option value="Euplotid_Nuclear">Euplotid_Nuclear</option>
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108 <option value="Bacterial_and_Plant_Plastid">Bacterial_and_Plant_Plastid</option>
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109 <option value="Alternative_Yeast_Nuclear">Alternative_Yeast_Nuclear</option>
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110 <option value="Ascidian_Mitochondrial">Ascidian_Mitochondrial</option>
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111 <option value="Alternative_Flatworm_Mitochondrial">Alternative_Flatworm_Mitochondrial</option>
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112 <option value="Blepharisma_Macronuclear">Blepharisma_Macronuclear</option>
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113 <option value="Chlorophycean_Mitochondrial">Chlorophycean_Mitochondrial</option>
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114 <option value="Trematode_Mitochondrial">Trematode_Mitochondrial</option>
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115 <option value="Scenedesmus_obliquus_Mitochondrial">Scenedesmus_obliquus_Mitochondrial</option>
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116 <option value="Thraustochytrium_Mitochondrial">Thraustochytrium_Mitochondrial</option>
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117 </param>
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118 </inputs>
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119 <outputs>
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120 <data name="snpeff_output" format="snpeffdb" label="@SNPEFF_VERSION@ database for ${genome_version}"/>
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121 <data name="output_fasta" format="fasta" label="Fasta sequences for ${genome_version}">
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122 <filter>input_type['input_type_selector'] == 'gb'</filter>
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123 <filter>input_type['fasta'] == 'yes'</filter>
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124 </data>
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125 </outputs>
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126 <tests>
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127 <test expect_num_outputs="2">
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128 <param name="genome_version" value="pBR322"/>
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129 <param name="input_type_selector" value="gb"/>
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130 <param name="input" value="pBR322.gbk" />
14
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131 <output name="snpeff_output">
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132 <assert_contents>
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133 <has_text text="pBR322" />
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134 </assert_contents>
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135 </output>
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136 <output name="output_fasta" value="pBR322_test1.fna"/>
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137 </test>
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138 <test expect_num_outputs="2">
19
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139 <param name="genome_version" value="pBR322"/>
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140 <param name="input_type_selector" value="gb"/>
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141 <param name="input" value="pBR322.gbk.gz" />
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142 <output name="snpeff_output">
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143 <assert_contents>
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144 <has_text text="pBR322" />
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145 </assert_contents>
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146 </output>
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147 <output name="output_fasta" value="pBR322_test1.fna"/>
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148 </test>
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149 <test expect_num_outputs="1">
19
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150 <param name="genome_version" value="pBR322"/>
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151 <param name="input_type_selector" value="gff"/>
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152 <param name="reference_source_selector" value="history"/>
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153 <param name="input_fasta" value="pBR322_test2.fna" />
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154 <param name="input" value="pBR322.gff3"/>
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155 <output name="snpeff_output">
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156 <assert_contents>
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157 <has_text text="pBR322" />
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158 </assert_contents>
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159 </output>
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160 </test>
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161 <test expect_num_outputs="1">
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162 <param name="genome_version" value="pBR322"/>
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163 <param name="input_type_selector" value="gff"/>
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164 <param name="reference_source_selector" value="history"/>
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165 <param name="input_fasta" value="pBR322_test2.fna.gz" />
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166 <param name="input" value="pBR322.gff3"/>
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167 <output name="snpeff_output">
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168 <assert_contents>
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169 <has_text text="pBR322" />
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170 </assert_contents>
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171 </output>
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172 </test>
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173 <test expect_num_outputs="1">
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174 <param name="genome_version" value="Saccharomyces_mito"/>
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175 <param name="input_type_selector" value="gtf"/>
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176 <param name="reference_source_selector" value="history"/>
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177 <param name="input_fasta" value="Saccharomyces_mito.fa.gz" />
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178 <param name="input" value="Saccharomyces_mito.gtf" />
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179 <output name="snpeff_output">
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180 <assert_contents>
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181 <has_text text="Saccharomyces_mito" />
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182 </assert_contents>
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183 </output>
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184 </test>
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185 </tests>
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186 <help><![CDATA[
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187 **What it does**
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188
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189 This tool uses `"snpEff build -genbank"` or `"snpEff build -gff3"` commands to create a snpEff database.
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190
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191 ------
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192
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193 .. class:: infomark
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194
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195 **Working with Genbank files**
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196
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197 Using Genbank data for creating databases has several advantages:
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198
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199 #. Genbank files contain annotations (such as locations of genes) together with sequences. This ensures that these two are in sync with each other.
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200 #. When you are analyzing small genomes (or not so small) it is much more convenient to create a database on the fly and use it.
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201
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202 .. class:: warningmark
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203
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204 SnpEff errors out on highly fragmented genomes containing multiple scaffolds. This is because a single gene may be split between multiple scaffolds causing SnpEff to crash. If this is happening use GFF route described below.
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205
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206 -------
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207
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208 **Genbank usage scenario**
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209
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210 Suppose you have a series of Illumina reads from an experiment involving *E. coli* K-12 MG1655. You want to map these reads to the reference genome of K-12 MG1655, call variants, and annotate them using snpEff. This tool enables you to follow the following analysis steps:
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211
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212 #. Go to `NCBI <http://www.ncbi.nlm.nih.gov>`_ page for K-12 MG1655 genome (note that all NCBI genomes have similar list of files associated with them).
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213 #. Copy URL for file with extension `gbff.gz`
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214 #. Paste the URL into upload tool and set datatype to `genbank.gz`.
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215 #. Use this tool to generate a snpEff database and FASTA sequences from the dataset you've uploaded during the previous step.
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216 #. Use your Illumina reads to map against FASTA dataset generated in the previous step using BWA-MEM.
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217 #. Call variants using **Freebayes**.
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218 #. Annotate vcf output of Freebayes with **SnpEff eff** using database generated at step 2 (using *Custom* option for **Genome source** parameter).
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219
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220 In this scenario Genbank dataset is used twice. First, it is used to produce FASTA sequences that are using by BWA to map against. Second, it is used to create snpEff database. This guarantees that you will not have any issues related to reference sequence naming.
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221
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222 -------
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223
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224 .. class:: infomark
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225
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226 **Working with GFF files**
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227
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228 Alternatively you can create a SnpEff database from `GFF3 <https://en.wikipedia.org/wiki/General_feature_format>`_ files downloaded from NCBI or any other source. Using GFF dataset for building SnpEff database requires two inputs:
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229
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230 #. The GFF file itself
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231 #. A genome in FASTA format
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232
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233 The GFF file contains coordinates of various features, but does not contain underlying sequences. This is why a FASTA file needs to be provided as well.
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234
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235 ------
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236
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237 **GFF usage scenario**
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238
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239 The following example also uses *E. coli* K-12 MG1655:
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240
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241 #. Go to `NCBI <http://www.ncbi.nlm.nih.gov>`_ page for K-12 MG1655 genome.
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242 #. Copy URLs for files with `gff.gz` and `fna.gz` extensions. The first file contains annotations in GFF3 format. The second file contains entire genome as a FASTA record.
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243 #. Paste URLs into upload tool and set datatypes to `gff3` and `fasta.gz` for annotations and genome, respectively.
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244 #. Use this tool to generate a snpEff database from the GFF dataset.
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245 #. Map your reads against the FASTA dataset and continue as described in the above example.
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246
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247
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248 @SNPEFF_IN_GALAXY_INFO@
9939612ba3cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit 5ab504d384299d8c2ed496650f1f9e4a887cd102
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249 @EXTERNAL_DOCUMENTATION@
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a7c106b938dd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit eea43430ff90fe6b13b295f6d5efb2208401a7ef
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250 ]]>
a7c106b938dd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit eea43430ff90fe6b13b295f6d5efb2208401a7ef
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251 </help>
a7c106b938dd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit eea43430ff90fe6b13b295f6d5efb2208401a7ef
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252 <expand macro="citations" />
a7c106b938dd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit eea43430ff90fe6b13b295f6d5efb2208401a7ef
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253 </tool>