Mercurial > repos > iuc > snpeff

annotate snpEff_create_db.xml @ 25:166c2e25a3b6 draft

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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
author iuc
date Tue, 15 Oct 2019 14:12:30 -0400
parents 9939612ba3cf
children b1b327f475ac
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rev   line source
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166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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1 <tool id="snpEff_build_gb" name="SnpEff build:" version="@WRAPPER_VERSION@.galaxy4">
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2 <description> database from Genbank or GFF record</description>
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3 <macros>
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4 <import>snpEff_macros.xml</import>
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5 </macros>
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6 <requirements>
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7 <expand macro="requirement" />
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8 <requirement type="package" version="3.6">python</requirement>
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9 <requirement type="package" version="1.70">biopython</requirement>
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10 <requirement type="package" version="0.4.15">python-magic</requirement>
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11 <requirement type="package" version="5.32">libmagic</requirement>
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12 </requirements>
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13 <expand macro="stdio" />
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14 <expand macro="version_command" />
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15 <command><![CDATA[
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16 #if str( $input_type.input_type_selector ) == "gb":
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17 #if str( $input_type.fasta ) == "yes":
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18 python3 '$__tool_directory__/gbk2fa.py' '${input_type.input_gbk}' '${output_fasta}'
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19 #if $input_type.remove_version:
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20 '${input_type.remove_version}'
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21 #end if
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22 &&
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23 #end if
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24 #end if
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26 mkdir -p '${snpeff_output.files_path}'/'${genome_version}' &&
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28 #if str( $input_type.input_type_selector ) == "gb":
718842784732 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit 999eca8a05f17ae567f99b8ca3394f2105491173
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29 #if $input_type.input_gbk.is_of_type("genbank"):
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30 ln -s '${input_type.input_gbk}' '${snpeff_output.files_path}'/'${genome_version}'/genes.gbk &&
718842784732 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit 999eca8a05f17ae567f99b8ca3394f2105491173
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31 #elif $input_type.input_gbk.is_of_type("genbank.gz"):
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32 ln -s '${input_type.input_gbk}' '${snpeff_output.files_path}'/'${genome_version}'/genes.gbk.gz &&
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33 #end if
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34 #elif str( $input_type.input_type_selector ) == "gff":
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35 #if $input_type.reference_source.reference_source_selector == "history":
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36 #if $input_type.reference_source.input_fasta.is_of_type("fasta"):
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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37 ln -s '${input_type.reference_source.input_fasta}' '${snpeff_output.files_path}'/'${genome_version}'/sequences.fa &&
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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38 #elif $input_type.reference_source.input_fasta.is_of_type("fasta.gz"):
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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39 ln -s '${input_type.reference_source.input_fasta}' '${snpeff_output.files_path}'/'${genome_version}'/sequences.fa.gz &&
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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40 #end if
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41 #elif $input_type.reference_source.reference_source_selector == "cached":
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42 ln -s '${input_type.reference_source.ref_file.fields.path}' '${snpeff_output.files_path}'/'${genome_version}'/sequences.fa &&
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43 #end if
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44 ln -s '${input_type.input_gff}' '${snpeff_output.files_path}'/'${genome_version}'/genes.gff &&
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45 #elif str( $input_type.input_type_selector ) == "gtf":
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46 #if $input_type.reference_source.reference_source_selector == "history":
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47 #if $input_type.reference_source.input_fasta.is_of_type("fasta"):
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48 ln -s '${input_type.reference_source.input_fasta}' '${snpeff_output.files_path}'/'${genome_version}'/sequences.fa &&
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49 #elif $input_type.reference_source.input_fasta.is_of_type("fasta.gz"):
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50 ln -s '${input_type.reference_source.input_fasta}' '${snpeff_output.files_path}'/'${genome_version}'/sequences.fa.gz &&
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51 #end if
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52 #elif $input_type.reference_source.reference_source_selector == "cached":
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53 ln -s '${input_type.reference_source.ref_file.fields.path}' '${snpeff_output.files_path}'/'${genome_version}'/sequences.fa &&
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54 #end if
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55 ln -s '${input_type.input_gtf}' '${snpeff_output.files_path}'/'${genome_version}'/genes.gtf &&
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56 #end if
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57
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58 snpEff @JAVA_OPTIONS@ build -v
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59 -configOption '${genome_version}'.genome='${genome_version}'
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60 -configOption '${genome_version}'.codonTable='${codon_table}'
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61 #if str( $input_type.input_type_selector ) == "gb":
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62 -genbank
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63 #elif str( $input_type.input_type_selector ) == "gff":
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64 -gff3
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65 #elif str( $input_type.input_type_selector ) == "gtf":
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66 -gtf22
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67 #end if
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68 -dataDir '${snpeff_output.files_path}' '${genome_version}' &&
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69 echo "${genome_version}.genome : ${genome_version}" >> '${snpeff_output.files_path}'/snpEff.config &&
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70 echo "${genome_version}.codonTable : ${codon_table}" >> '${snpeff_output.files_path}'/snpEff.config
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72 ]]></command>
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73 <inputs>
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74 <param name="genome_version" type="text" value="" label="Name for the database" help="For E. coli K12 you may want to use 'EcK12' etc.">
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75 <validator type="empty_field" message="A genome version name is required" />
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76 </param>
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77 <conditional name="input_type">
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78 <param name="input_type_selector" type="select" display="radio" label="Input annotations are in" help="Specify format for annotations you are using to create SnpEff database">
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79 <option value="gb" selected="true">GenBank</option>
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parents: 17
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80 <option value="gff">GFF</option>
25
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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parents: 21
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81 <option value="gtf">GTF</option>
19
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parents: 17
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82 </param>
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parents: 17
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83 <when value="gb">
718842784732 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit 999eca8a05f17ae567f99b8ca3394f2105491173
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parents: 17
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84 <param name="input_gbk" type="data" format="genbank,genbank.gz" label="Genbank dataset to build database from" help="This Genbank file will be used to generate snpEff database"/>
718842784732 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit 999eca8a05f17ae567f99b8ca3394f2105491173
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parents: 17
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85 <param name="fasta" type="select" display="radio" label="Parse Genbank into Fasta" help="This will generate an additional dataset containing all sequences from Genbank file in FASTA format">
718842784732 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit 999eca8a05f17ae567f99b8ca3394f2105491173
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parents: 17
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86 <option value="yes" selected="true">Yes</option>
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parents: 17
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87 <option value="no">No</option>
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parents: 17
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88 </param>
21
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parents: 19
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89 <param argument="--remove_version" type="boolean" truevalue="--remove_version" falsevalue="" checked="true" label="Remove sequence version label?" help="Genbank sequences have vesion numbers such as B000564.2. This option removes them leaving only B000564" />
19
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parents: 17
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90 </when>
21
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parents: 19
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91 <when value="gff">
19
718842784732 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit 999eca8a05f17ae567f99b8ca3394f2105491173
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parents: 17
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92 <param name="input_gff" type="data" format="gff3" label="GFF dataset to build database from" help="This GFF file will be used to generate snpEff database"/>
25
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parents: 21
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93 <conditional name="reference_source">
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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parents: 21
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94 <param name="reference_source_selector" type="select" label="Choose the source for the reference genome">
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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parents: 21
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95 <option value="cached">Locally cached</option>
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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parents: 21
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96 <option value="history">History</option>
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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parents: 21
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97 </param>
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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parents: 21
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98 <when value="cached">
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parents: 21
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99 <param name="ref_file" type="select" label="Select reference genome">
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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parents: 21
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100 <options from_data_table="fasta_indexes"/>
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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parents: 21
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101 </param>
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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parents: 21
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102 </when>
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
iuc
parents: 21
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103 <when value="history">
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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parents: 21
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104 <param name="input_fasta" type="data" format="fasta,fasta.gz" label="Genome in FASTA format" help="This dataset is required for generating SnpEff database. See help section below."/>
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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parents: 21
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105 </when>
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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parents: 21
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106 </conditional>
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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parents: 21
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107 </when>
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parents: 21
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108 <when value="gtf">
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109 <param name="input_gtf" type="data" format="gtf" label="GTF dataset to build database from" help="This GTF file will be used to generate snpEff database"/>
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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110 <conditional name="reference_source">
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parents: 21
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111 <param name="reference_source_selector" type="select" label="Choose the source for the reference genome">
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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112 <option value="cached">Locally cached</option>
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parents: 21
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113 <option value="history">History</option>
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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parents: 21
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114 </param>
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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parents: 21
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115 <when value="cached">
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parents: 21
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116 <param name="ref_file" type="select" label="Select reference genome">
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117 <options from_data_table="fasta_indexes"/>
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118 </param>
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119 </when>
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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120 <when value="history">
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121 <param name="input_fasta" type="data" format="fasta,fasta.gz" label="Genome in FASTA format" help="This dataset is required for generating SnpEff database. See help section below."/>
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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122 </when>
166c2e25a3b6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit aeb660e92b9eb60dc5f90a2078f552018cca96e0"
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parents: 21
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123 </conditional>
19
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parents: 17
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124 </when>
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125 </conditional>
14
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126 <param name="codon_table" type="select" label="Select genetic code for this sequence" help="If this sequence uses non-standard genetic code, select one from these options">
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127 <option selected="true" value="Standard">Standard</option>
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128 <option value="Vertebrate_Mitochondrial">Vertebrate_Mitochondrial</option>
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129 <option value="Yeast_Mitochondrial">Yeast_Mitochondrial</option>
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130 <option value="Mold_Mitochondrial">Mold_Mitochondrial</option>
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131 <option value="Protozoan_Mitochondrial">Protozoan_Mitochondrial</option>
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132 <option value="Coelenterate">Coelenterate</option>
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133 <option value="Mitochondrial">Mitochondrial</option>
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134 <option value="Mycoplasma">Mycoplasma</option>
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135 <option value="Spiroplasma">Spiroplasma</option>
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136 <option value="Invertebrate_Mitochondrial">Invertebrate_Mitochondrial</option>
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137 <option value="Ciliate_Nuclear">Ciliate_Nuclear</option>
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138 <option value="Dasycladacean_Nuclear">Dasycladacean_Nuclear</option>
a7c106b938dd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit eea43430ff90fe6b13b295f6d5efb2208401a7ef
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139 <option value="Hexamita_Nuclear">Hexamita_Nuclear</option>
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140 <option value="Echinoderm_Mitochondrial">Echinoderm_Mitochondrial</option>
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141 <option value="Flatworm_Mitochondrial">Flatworm_Mitochondrial</option>
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142 <option value="Euplotid_Nuclear">Euplotid_Nuclear</option>
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143 <option value="Bacterial_and_Plant_Plastid">Bacterial_and_Plant_Plastid</option>
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144 <option value="Alternative_Yeast_Nuclear">Alternative_Yeast_Nuclear</option>
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145 <option value="Ascidian_Mitochondrial">Ascidian_Mitochondrial</option>
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146 <option value="Alternative_Flatworm_Mitochondrial">Alternative_Flatworm_Mitochondrial</option>
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147 <option value="Blepharisma_Macronuclear">Blepharisma_Macronuclear</option>
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148 <option value="Chlorophycean_Mitochondrial">Chlorophycean_Mitochondrial</option>
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149 <option value="Trematode_Mitochondrial">Trematode_Mitochondrial</option>
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150 <option value="Scenedesmus_obliquus_Mitochondrial">Scenedesmus_obliquus_Mitochondrial</option>
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151 <option value="Thraustochytrium_Mitochondrial">Thraustochytrium_Mitochondrial</option>
a7c106b938dd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/snpeff commit eea43430ff90fe6b13b295f6d5efb2208401a7ef
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152 </param>
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153 </inputs>
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154 <outputs>
21
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155 <data name="snpeff_output" format="snpeffdb" label="@SNPEFF_VERSION@ database for ${genome_version}"/>
15
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156 <data name="output_fasta" format="fasta" label="Fasta sequences for ${genome_version}">
19
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157 <filter>input_type['input_type_selector'] == 'gb'</filter>
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158 <filter>input_type['fasta'] == 'yes'</filter>
14
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159 </data>
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160 </outputs>
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161 <tests>
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162 <test>
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163 <param name="genome_version" value="pBR322"/>
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164 <param name="input_type_selector" value="gb"/>
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165 <param name="input_gbk" value="pBR322.gbk" />
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166 <output name="snpeff_output">
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167 <assert_contents>
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168 <has_text text="pBR322" />
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169 </assert_contents>
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170 </output>
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171 <output name="output_fasta" value="pBR322_test1.fna"/>
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172 </test>
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173 <test>
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174 <param name="genome_version" value="pBR322"/>
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175 <param name="input_type_selector" value="gb"/>
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176 <param name="input_gbk" value="pBR322.gbk.gz" />
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177 <output name="snpeff_output">
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178 <assert_contents>
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179 <has_text text="pBR322" />
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180 </assert_contents>
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181 </output>
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182 <output name="output_fasta" value="pBR322_test1.fna"/>
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183 </test>
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184 <test>
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185 <param name="genome_version" value="pBR322"/>
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186 <param name="input_type_selector" value="gff"/>
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187 <param name="reference_source_selector" value="history"/>
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188 <param name="input_fasta" value="pBR322_test2.fna" />
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189 <param name="input_gff" value="pBR322.gff3" />
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190 <output name="snpeff_output">
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191 <assert_contents>
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192 <has_text text="pBR322" />
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193 </assert_contents>
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194 </output>
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195 </test>
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196 <test>
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197 <param name="genome_version" value="pBR322"/>
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198 <param name="input_type_selector" value="gff"/>
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199 <param name="reference_source_selector" value="history"/>
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200 <param name="input_fasta" value="pBR322_test2.fna.gz" />
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201 <param name="input_gff" value="pBR322.gff3" />
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202 <output name="snpeff_output">
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203 <assert_contents>
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204 <has_text text="pBR322" />
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205 </assert_contents>
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206 </output>
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207 </test>
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208 <test>
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209 <param name="genome_version" value="Saccharomyces_mito"/>
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210 <param name="input_type_selector" value="gtf"/>
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211 <param name="reference_source_selector" value="history"/>
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212 <param name="input_fasta" value="Saccharomyces_mito.fa.gz" />
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213 <param name="input_gtf" value="Saccharomyces_mito.gtf" />
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214 <output name="snpeff_output">
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215 <assert_contents>
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216 <has_text text="Saccharomyces_mito" />
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217 </assert_contents>
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218 </output>
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219 </test>
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220 </tests>
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221 <help><![CDATA[
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222 **What it does**
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223
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224 This tool uses `"snpEff build -genbank"` or `"snpEff build -gff3"` commands to create a snpEff database.
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225
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226 ------
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227
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228 .. class:: infomark
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229
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230 **Working with Genbank files**
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231
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232 Using Genbank data for creating databases has several advantages:
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233
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234 #. Genbank files contain annotations (such as locations of genes) together with sequences. This ensures that these two are in sync with each other.
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235 #. When you are analyzing small genomes (or not so small) it is much more convenient to create a database on the fly and use it.
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236
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237 .. class:: warningmark
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238
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239 SnpEff errors out on highly fragmented genomes containing multiple scaffolds. This is because a single gene may be split between multiple scaffolds causing SnpEff to crash. If this is happening use GFF route described below.
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240
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241 -------
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242
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243 **Genbank usage scenario**
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244
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245 Suppose you have a series of Illumina reads from an experiment involving *E. coli* K-12 MG1655. You want to map these reads to the reference genome of K-12 MG1655, call variants, and annotate them using snpEff. This tool enables you to follow the following analysis steps:
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246
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247 #. Go to `NCBI <http://www.ncbi.nlm.nih.gov>`_ page for K-12 MG1655 genome (note that all NCBI genomes have similar list of files associated with them).
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248 #. Copy URL for file with extension `gbff.gz`
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249 #. Paste the URL into upload tool and set datatype to `genbank.gz`.
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250 #. Use this tool to generate a snpEff database and FASTA sequences from the dataset you've uploaded during the previous step.
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251 #. Use your Illumina reads to map against FASTA dataset generated in the previous step using BWA-MEM.
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252 #. Call variants using **Freebayes**.
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253 #. Annotate vcf output of Freebayes with **SnpEff eff** using database generated at step 2 (using *Custom* option for **Genome source** parameter).
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254
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255 In this scenario Genbank dataset is used twice. First, it is used to produce FASTA sequences that are using by BWA to map against. Second, it is used to create snpEff database. This guarantees that you will not have any issues related to reference sequence naming.
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256
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257 -------
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258
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259 .. class:: infomark
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260
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261 **Working with GFF files**
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262
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263 Alternatively you can create a SnpEff database from `GFF3 <https://en.wikipedia.org/wiki/General_feature_format>`_ files downloaded from NCBI or any other source. Using GFF dataset for building SnpEff database requires two inputs:
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264
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265 #. The GFF file itself
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266 #. A genome in FASTA format
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267
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268 The GFF file contains coordinates of various features, but does not contain underlying sequences. This is why a FASTA file needs to be provided as well.
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270 ------
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271
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272 **GFF usage scenario**
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273
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274 The following example also uses *E. coli* K-12 MG1655:
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275
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276 #. Go to `NCBI <http://www.ncbi.nlm.nih.gov>`_ page for K-12 MG1655 genome.
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277 #. Copy URLs for files with `gff.gz` and `fna.gz` extensions. The first file contains annotations in GFF3 format. The second file contains entire genome as a FASTA record.
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278 #. Paste URLs into upload tool and set datatypes to `gff3` and `fasta.gz` for annotations and genome, respectively.
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279 #. Use this tool to generate a snpEff database from the GFF dataset.
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280 #. Map your reads against the FASTA dataset and continue as described in the above example.
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281
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282
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283 @SNPEFF_IN_GALAXY_INFO@
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284 @EXTERNAL_DOCUMENTATION@
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285 ]]>
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286 </help>
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287 <expand macro="citations" />
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288 </tool>