Mercurial > repos > iuc > mothur_pre_cluster

annotate pre.cluster.xml @ 3:d6bed1e2df21 draft

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
author iuc
date Wed, 14 Feb 2018 09:14:38 -0500
parents e277ab688993
children 4af9561f3c7c
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4b5b8a603f52 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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1 <tool profile="16.07" id="mothur_pre_cluster" name="Pre.cluster" version="@WRAPPER_VERSION@.0">
4b5b8a603f52 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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2 <description>Remove sequences due to pyrosequencing errors</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="requirements"/>
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7 <expand macro="stdio"/>
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8 <expand macro="version_command"/>
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9 <command><![CDATA[
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10 @SHELL_OPTIONS@
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12 ## create symlinks to input datasets
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13 ln -s '$fasta' fasta.dat &&
d6bed1e2df21 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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14 ln -s '$name' name.dat &&
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15 ln -s '$group' group.dat &&
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16
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17 echo 'pre.cluster(
d6bed1e2df21 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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18 fasta=fasta.dat,
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19 #if $name.is_of_type("mothur.names"):
d6bed1e2df21 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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20 name=name.dat,
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21 #elif $name.is_of_type("mothur.count_table"):
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22 count=name.dat,
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23 #end if
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24 #if $group:
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25 group=group.dat,
d6bed1e2df21 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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26 #end if
d6bed1e2df21 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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27 diffs=$diffs,
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28 match=$match,
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29 mismatch=$mismatch,
d6bed1e2df21 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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30 gapopen=$gapopen,
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31 gapextend=$gapextend,
d6bed1e2df21 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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32 topdown=$topdown,
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33 processors='\${GALAXY_SLOTS:-8}'
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34 )'
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35 | sed 's/ //g' ## mothur trips over whitespace
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36 | mothur
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37 | tee mothur.out.log &&
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38
3
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39 cat fasta* &&
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40
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41 if [ -f fasta.precluster.map ]; then mv fasta.precluster.map fasta.precluster.fasta.map; fi
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42 ]]></command>
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43 <inputs>
3
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44 <param argument="fasta" type="data" format="fasta" label="fasta - Sequence Fasta"/>
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45 <param argument="name" type="data" format="mothur.names,mothur.count_table" optional="true" label="name file or count table - Sequences Name reference"/>
d6bed1e2df21 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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46 <param argument="group" type="data" format="mothur.groups" optional="true" label="group - Sequences Name reference"/>
d6bed1e2df21 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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47 <param argument="diffs" type="integer" value="1" min="0" label="diffs - Number of mismatched bases to allow between sequences in a group (default 1)"/>
d6bed1e2df21 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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48 <param argument="match" type="integer" value="1" label="match - reward for a match (default 1)"/>
d6bed1e2df21 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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49 <param argument="mismatch" type="integer" value="-1" label="mismatch - penalty for a mismatch (default -1)"/>
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50 <param argument="gapopen" type="integer" value="-2" label="gapopen - penalty for opening a gap (default -2)"/>
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51 <param argument="gapextend" type="integer" value="-1" label="gapextend - penalty for extending a gap (default -1)"/>
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52 <param argument="topdown" type="boolean" truevalue="true" falsevalue="false" checked="true" label="topdown"
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53 help="cluster from largest abundance to smallest or vice versa. Default=Yes, meaning largest to smallest"/>
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54 </inputs>
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55 <outputs>
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56 <expand macro="logfile-output"/>
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57 <data name="fasta_out" format_source="fasta" from_work_dir="fasta*.precluster.dat" label="${tool.name} on ${on_string}: precluster.fasta"/>
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58 <data name="names_out" format="mothur.names" from_work_dir="fasta*.precluster.names" label="${tool.name} on ${on_string}: precluster.names">
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59 <filter>name.ext != "mothur.count_table"</filter>
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60 </data>
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61 <data name="count_out" format="mothur.count_table" from_work_dir="fasta*.precluster.count_table" label="${tool.name} on ${on_string}: precluster.count_table">
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62 <filter>name.ext == "mothur.count_table"</filter>
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63 </data>
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64 <collection name="map_out" type="list" label="${tool.name} on ${on_string}: precluster.map">
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65 <discover_datasets pattern="fasta\.precluster\.(?P&lt;designation&gt;.*)\.map" format="tabular"/>
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66 </collection>
0
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67 </outputs>
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68 <tests>
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69 <test><!-- test with fasta and names file -->
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70 <param name="fasta" value="amazon.fasta_head" ftype="fasta"/>
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71 <param name="name" value="amazon.head.names" ftype="mothur.names"/>
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72 <output name="fasta_out" ftype="fasta">
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73 <assert_contents>
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74 <expand macro="test-fasta-format"/>
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75 <has_text text="U68589"/>
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76 </assert_contents>
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77 </output>
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78 <output name="names_out" ftype="mothur.names">
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79 <assert_contents>
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80 <expand macro="test-names-format"/>
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81 <has_line_matching expression="^U68589\tU68589$"/>
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82 </assert_contents>
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83 </output>
1
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84 <output_collection name="map_out" count="1">
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85 <element name="fasta" ftype="tabular">
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86 <assert_contents>
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87 <has_text text="ideal_seq_1"/>
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88 <has_text text="U68589"/>
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89 </assert_contents>
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90 </element>
1
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91 </output_collection>
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92 <expand macro="logfile-test"/>
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93 </test>
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94 <test><!-- test with fasta and count table -->
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95 <param name="fasta" value="amazon.fasta_head" ftype="fasta"/>
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96 <param name="name" value="amazon.count_table" ftype="mothur.count_table"/>
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97 <output name="fasta_out" ftype="fasta">
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98 <assert_contents>
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99 <expand macro="test-fasta-format"/>
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100 <has_text text="U68589"/>
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101 </assert_contents>
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102 </output>
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103 <output name="count_out" ftype="mothur.count_table">
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104 <assert_contents>
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105 <expand macro="test-count-format"/>
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106 <has_text text="U68589"/>
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107 </assert_contents>
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108 </output>
1
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109 <output_collection name="map_out" count="1">
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110 <element name="fasta" ftype="tabular">
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111 <assert_contents>
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112 <has_text text="ideal_seq_1"/>
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113 <has_text text="U68589"/>
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114 </assert_contents>
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115 </element>
1
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116 </output_collection>
0
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117 <expand macro="logfile-test"/>
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118 </test>
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119 </tests>
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120 <help><![CDATA[
0
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121
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122 @MOTHUR_OVERVIEW@
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123
1
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124 **Command Documentation**
0
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125
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126 The pre.cluster_ command implements a pseudo-single linkage algorithm with the goal of removing sequences that
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127 are likely due to pyrosequencing errors. The basic idea is that abundant sequences are more likely to generate
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128 erroneous sequences than rare sequences. With that in mind, the algorithm proceeds by ranking sequences in order
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129 of their abundance. Then we walk through the list of sequences looking for rarer sequences that are within some
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130 threshold of the original sequence. Those that are within the threshold are merged with the larger sequence.
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131 The original Huse method performs this task on a distance matrix, whereas we do it based on the original sequences.
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132 The advantage of our approach is that the algorithm works on aligned sequences instead of a distance matrix.
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133 This is advantageous because by pre-clustering you remove a large number of sequences making the distance
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134 calculation much faster.
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135
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136 .. _pre.cluster: https://www.mothur.org/wiki/Pre.cluster
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137
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138 v1.24.0: Updated to mothur 1.33, added count and topdown parameter
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139
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140 ]]></help>
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141 <expand macro="citations"/>
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142 </tool>