annotate pre.cluster.xml @ 1:e277ab688993 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
author iuc
date Thu, 18 May 2017 18:09:17 -0400
parents 4b5b8a603f52
children d6bed1e2df21
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1 <tool profile="16.07" id="mothur_pre_cluster" name="Pre.cluster" version="@WRAPPER_VERSION@.0">
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2 <description>Remove sequences due to pyrosequencing errors</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="requirements"/>
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7 <expand macro="stdio"/>
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8 <expand macro="version_command"/>
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9 <command><![CDATA[
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10 @SHELL_OPTIONS@
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11
0
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12 ## create symlinks to input datasets
4b5b8a603f52 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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13 ln -s "$fasta" fasta.dat &&
4b5b8a603f52 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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14 ln -s "$name" name.dat &&
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15 ln -s "$group" group.dat &&
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16
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17 echo 'pre.cluster(
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18 fasta=fasta.dat,
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19 #if $name.is_of_type("mothur.names"):
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20 name=name.dat,
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21 #elif $name.is_of_type("mothur.count_table"):
4b5b8a603f52 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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22 count=name.dat,
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23 #end if
4b5b8a603f52 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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24 #if $group:
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25 group=group.dat,
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26 #end if
4b5b8a603f52 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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27 diffs=$diffs,
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28 match=$match,
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29 mismatch=$mismatch,
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30 gapopen=$gapopen,
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31 gapextend=$gapextend,
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32 topdown=$topdown,
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33 processors='\${GALAXY_SLOTS:-8}'
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34 )'
4b5b8a603f52 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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35 | sed 's/ //g' ## mothur trips over whitespace
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36 | mothur
1
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37 | tee mothur.out.log &&
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38
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39 if [ -f fasta.precluster.map ]; then mv fasta.precluster.map fasta.precluster.fasta.map; fi
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40 ]]></command>
4b5b8a603f52 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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41 <inputs>
4b5b8a603f52 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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42 <param name="fasta" type="data" format="fasta" label="fasta - Sequence Fasta"/>
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43 <param name="name" type="data" format="mothur.names,mothur.count_table" optional="true" label="name file or count table - Sequences Name reference"/>
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44 <param name="group" type="data" format="mothur.groups" optional="true" label="group - Sequences Name reference"/>
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45 <param name="diffs" type="integer" value="1" min="0" label="diffs - Number of mismatched bases to allow between sequences in a group (default 1)"/>
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46 <param name="match" type="integer" value="1" label="match - reward for a match (default 1)"/>
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47 <param name="mismatch" type="integer" value="-1" label="mismatch - penalty for a mismatch (default -1)"/>
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48 <param name="gapopen" type="integer" value="-2" label="gapopen - penalty for opening a gap (default -2)"/>
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49 <param name="gapextend" type="integer" value="-1" label="gapextend - penalty for extending a gap (default -1)"/>
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50 <param name="topdown" type="boolean" truevalue="true" falsevalue="false" checked="true" label="topdown" help="cluster from largest abundance to smallest or vice versa. Default=Yes, meaning largest to smallest"/>
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51 </inputs>
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52 <outputs>
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53 <expand macro="logfile-output"/>
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54 <data name="fasta_out" format_source="fasta" from_work_dir="fasta*.precluster.dat" label="${tool.name} on ${on_string}: precluster.fasta"/>
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55 <data name="names_out" format="mothur.names" from_work_dir="fasta*.precluster.names" label="${tool.name} on ${on_string}: precluster.names">
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56 <filter>name.ext != "mothur.count_table"</filter>
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57 </data>
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58 <data name="count_out" format="mothur.count_table" from_work_dir="fasta*.precluster.count_table" label="${tool.name} on ${on_string}: precluster.count_table">
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59 <filter>name.ext == "mothur.count_table"</filter>
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60 </data>
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61 <collection name="map_out" type="list" label="${tool.name} on ${on_string}: precluster.map">
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62 <discover_datasets pattern="fasta\.precluster\.(?P&lt;designation&gt;.*)\.map" format="tabular"/>
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63 </collection>
0
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64 </outputs>
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65 <tests>
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66 <test><!-- test with fasta and names file -->
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67 <param name="fasta" value="amazon.fasta_head" ftype="fasta"/>
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68 <param name="name" value="amazon.head.names" ftype="mothur.names"/>
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69 <output name="fasta_out" md5="d5d37ace0f2a5c020edd0aa9eb7aca9b" ftype="fasta"/>
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70 <output name="names_out" md5="51090fe5e111920ec1003c2073532d96" ftype="mothur.names"/>
1
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71 <output_collection name="map_out" count="1">
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72 <element name="fasta" md5="c2ca7b0d45aa3881c7bd5cb8a44c853b" ftype="tabular"/>
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73 </output_collection>
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74 <expand macro="logfile-test"/>
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75 </test>
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76 <test><!-- test with fasta and count table -->
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77 <param name="fasta" value="amazon.fasta_head" ftype="fasta"/>
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78 <param name="name" value="amazon.count_table" ftype="mothur.count_table"/>
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79 <output name="fasta_out" md5="d5d37ace0f2a5c020edd0aa9eb7aca9b" ftype="fasta"/>
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80 <output name="count_out" md5="9831e271b9a96f91ad700f84aeed03a5" ftype="mothur.count_table"/>
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81 <output_collection name="map_out" count="1">
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82 <element name="fasta" md5="c2ca7b0d45aa3881c7bd5cb8a44c853b" ftype="tabular"/>
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83 </output_collection>
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84 <expand macro="logfile-test"/>
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85 </test>
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86 </tests>
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87 <help>
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88 <![CDATA[
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89
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90 @MOTHUR_OVERVIEW@
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91
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92 **Command Documentation**
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93
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94 The pre.cluster_ command implements a pseudo-single linkage algorithm with the goal of removing sequences that are likely due to pyrosequencing errors. The basic idea is that abundant sequences are more likely to generate erroneous sequences than rare sequences. With that in mind, the algorithm proceeds by ranking sequences in order of their abundance. Then we walk through the list of sequences looking for rarer sequences that are within some threshold of the original sequence. Those that are within the threshold are merged with the larger sequence. The original Huse method performs this task on a distance matrix, whereas we do it based on the original sequences. The advantage of our approach is that the algorithm works on aligned sequences instead of a distance matrix. This is advantageous because by pre-clustering you remove a large number of sequences making the distance calculation much faster.
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95
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96 .. _pre.cluster: https://www.mothur.org/wiki/Pre.cluster
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97
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98 v1.24.0: Updated to mothur 1.33, added count and topdown parameter
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99 ]]>
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100 </help>
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101 <expand macro="citations"/>
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102 </tool>