Mercurial > repos > iuc > mothur_pre_cluster
annotate pre.cluster.xml @ 1:e277ab688993 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
| author | iuc |
|---|---|
| date | Thu, 18 May 2017 18:09:17 -0400 |
| parents | 4b5b8a603f52 |
| children | d6bed1e2df21 |
| rev | line source |
|---|---|
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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changeset
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1 <tool profile="16.07" id="mothur_pre_cluster" name="Pre.cluster" version="@WRAPPER_VERSION@.0"> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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2 <description>Remove sequences due to pyrosequencing errors</description> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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3 <macros> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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4 <import>macros.xml</import> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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5 </macros> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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6 <expand macro="requirements"/> |
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1
e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
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7 <expand macro="stdio"/> |
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0
4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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8 <expand macro="version_command"/> |
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1
e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
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9 <command><![CDATA[ |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
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10 @SHELL_OPTIONS@ |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
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11 |
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0
4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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12 ## create symlinks to input datasets |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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13 ln -s "$fasta" fasta.dat && |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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14 ln -s "$name" name.dat && |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
parents:
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15 ln -s "$group" group.dat && |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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16 |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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changeset
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17 echo 'pre.cluster( |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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18 fasta=fasta.dat, |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
parents:
diff
changeset
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19 #if $name.is_of_type("mothur.names"): |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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diff
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20 name=name.dat, |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
parents:
diff
changeset
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21 #elif $name.is_of_type("mothur.count_table"): |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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22 count=name.dat, |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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23 #end if |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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changeset
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24 #if $group: |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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diff
changeset
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25 group=group.dat, |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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diff
changeset
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26 #end if |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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diff
changeset
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27 diffs=$diffs, |
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1
e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
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28 match=$match, |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
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29 mismatch=$mismatch, |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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30 gapopen=$gapopen, |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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31 gapextend=$gapextend, |
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0
4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
parents:
diff
changeset
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32 topdown=$topdown, |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
parents:
diff
changeset
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33 processors='\${GALAXY_SLOTS:-8}' |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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34 )' |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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changeset
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35 | sed 's/ //g' ## mothur trips over whitespace |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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36 | mothur |
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1
e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
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37 | tee mothur.out.log && |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
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38 |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
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changeset
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39 if [ -f fasta.precluster.map ]; then mv fasta.precluster.map fasta.precluster.fasta.map; fi |
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0
4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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changeset
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40 ]]></command> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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diff
changeset
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41 <inputs> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
parents:
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changeset
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42 <param name="fasta" type="data" format="fasta" label="fasta - Sequence Fasta"/> |
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1
e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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43 <param name="name" type="data" format="mothur.names,mothur.count_table" optional="true" label="name file or count table - Sequences Name reference"/> |
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0
4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
parents:
diff
changeset
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44 <param name="group" type="data" format="mothur.groups" optional="true" label="group - Sequences Name reference"/> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
parents:
diff
changeset
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45 <param name="diffs" type="integer" value="1" min="0" label="diffs - Number of mismatched bases to allow between sequences in a group (default 1)"/> |
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1
e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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46 <param name="match" type="integer" value="1" label="match - reward for a match (default 1)"/> |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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47 <param name="mismatch" type="integer" value="-1" label="mismatch - penalty for a mismatch (default -1)"/> |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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48 <param name="gapopen" type="integer" value="-2" label="gapopen - penalty for opening a gap (default -2)"/> |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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49 <param name="gapextend" type="integer" value="-1" label="gapextend - penalty for extending a gap (default -1)"/> |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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50 <param name="topdown" type="boolean" truevalue="true" falsevalue="false" checked="true" label="topdown" help="cluster from largest abundance to smallest or vice versa. Default=Yes, meaning largest to smallest"/> |
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0
4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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51 </inputs> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
parents:
diff
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52 <outputs> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
parents:
diff
changeset
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53 <expand macro="logfile-output"/> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
parents:
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54 <data name="fasta_out" format_source="fasta" from_work_dir="fasta*.precluster.dat" label="${tool.name} on ${on_string}: precluster.fasta"/> |
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1
e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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55 <data name="names_out" format="mothur.names" from_work_dir="fasta*.precluster.names" label="${tool.name} on ${on_string}: precluster.names"> |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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changeset
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56 <filter>name.ext != "mothur.count_table"</filter> |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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57 </data> |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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58 <data name="count_out" format="mothur.count_table" from_work_dir="fasta*.precluster.count_table" label="${tool.name} on ${on_string}: precluster.count_table"> |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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59 <filter>name.ext == "mothur.count_table"</filter> |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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60 </data> |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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61 <collection name="map_out" type="list" label="${tool.name} on ${on_string}: precluster.map"> |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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62 <discover_datasets pattern="fasta\.precluster\.(?P<designation>.*)\.map" format="tabular"/> |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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63 </collection> |
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0
4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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diff
changeset
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64 </outputs> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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65 <tests> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
parents:
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66 <test><!-- test with fasta and names file --> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
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67 <param name="fasta" value="amazon.fasta_head" ftype="fasta"/> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
parents:
diff
changeset
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68 <param name="name" value="amazon.head.names" ftype="mothur.names"/> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
parents:
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changeset
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69 <output name="fasta_out" md5="d5d37ace0f2a5c020edd0aa9eb7aca9b" ftype="fasta"/> |
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4b5b8a603f52
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
iuc
parents:
diff
changeset
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70 <output name="names_out" md5="51090fe5e111920ec1003c2073532d96" ftype="mothur.names"/> |
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1
e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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71 <output_collection name="map_out" count="1"> |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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72 <element name="fasta" md5="c2ca7b0d45aa3881c7bd5cb8a44c853b" ftype="tabular"/> |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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73 </output_collection> |
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e277ab688993
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
iuc
parents:
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74 <expand macro="logfile-test"/> |
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e277ab688993
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75 </test> |
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76 <test><!-- test with fasta and count table --> |
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77 <param name="fasta" value="amazon.fasta_head" ftype="fasta"/> |
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78 <param name="name" value="amazon.count_table" ftype="mothur.count_table"/> |
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79 <output name="fasta_out" md5="d5d37ace0f2a5c020edd0aa9eb7aca9b" ftype="fasta"/> |
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80 <output name="count_out" md5="9831e271b9a96f91ad700f84aeed03a5" ftype="mothur.count_table"/> |
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81 <output_collection name="map_out" count="1"> |
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82 <element name="fasta" md5="c2ca7b0d45aa3881c7bd5cb8a44c853b" ftype="tabular"/> |
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83 </output_collection> |
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84 <expand macro="logfile-test"/> |
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85 </test> |
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86 </tests> |
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87 <help> |
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88 <![CDATA[ |
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89 |
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90 @MOTHUR_OVERVIEW@ |
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91 |
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92 **Command Documentation** |
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93 |
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94 The pre.cluster_ command implements a pseudo-single linkage algorithm with the goal of removing sequences that are likely due to pyrosequencing errors. The basic idea is that abundant sequences are more likely to generate erroneous sequences than rare sequences. With that in mind, the algorithm proceeds by ranking sequences in order of their abundance. Then we walk through the list of sequences looking for rarer sequences that are within some threshold of the original sequence. Those that are within the threshold are merged with the larger sequence. The original Huse method performs this task on a distance matrix, whereas we do it based on the original sequences. The advantage of our approach is that the algorithm works on aligned sequences instead of a distance matrix. This is advantageous because by pre-clustering you remove a large number of sequences making the distance calculation much faster. |
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95 |
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96 .. _pre.cluster: https://www.mothur.org/wiki/Pre.cluster |
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97 |
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98 v1.24.0: Updated to mothur 1.33, added count and topdown parameter |
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99 ]]> |
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100 </help> |
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101 <expand macro="citations"/> |
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102 </tool> |
