annotate fastp.xml @ 14:9047af1bc797 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 17a0a67dfbc7541a7fe3618cfbbb71d814042889
author iuc
date Fri, 15 Nov 2024 15:31:38 +0000
parents 2d605e86b5d7
children 515315f1a8a0
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9047af1bc797 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 17a0a67dfbc7541a7fe3618cfbbb71d814042889
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1 <tool id="fastp" name="fastp" version="@TOOL_VERSION@+galaxy3" profile="23.1">
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2 <description>fast all-in-one preprocessing for FASTQ files</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="biotools" />
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7 <requirements>
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8 <requirement type="package" version="@TOOL_VERSION@">fastp</requirement>
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9 </requirements>
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10 <version_command>fastp -v</version_command>
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11 <command detect_errors="exit_code"><![CDATA[
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12 #import re
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13
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14 ## Link input files
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15
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16 #set ext = '.fastqsanger'
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17
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18 #if $single_paired.single_paired_selector == 'paired_collection'
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19 #if $single_paired.paired_input.forward.is_of_type('fastqsanger.gz')
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20 #set ext = '.fastqsanger.gz'
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21 #end if
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22 #set $in1 = $single_paired.paired_input.forward
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23 #set $in2 = $single_paired.paired_input.reverse
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24 #set $in1_name = re.sub('[^\w\-\s]', '_', str($single_paired.paired_input.name)) + $ext
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25 #set $in2_name = re.sub('[^\w\-\s]', '_', str("%s_%s" % ($single_paired.paired_input.name, "R2"))) + $ext
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26 #set out1 = $output_paired_coll.forward
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27 #set out2 = $output_paired_coll.reverse
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28 ln -sf '$in1' '$in1_name' &&
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29 ln -sf '$in2' '$in2_name' &&
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30 #else
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31 #if $in1.is_of_type('fastqsanger.gz')
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32 #set ext = '.fastqsanger.gz'
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33 #end if
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34
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35 #set $in1_name = re.sub('[^\w\-\s]', '_', str($in1.element_identifier)) + $ext
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36 ln -sf '$in1' '$in1_name' &&
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37
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38 #if str($single_paired.single_paired_selector) == 'paired':
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39 #set $in2_name = re.sub('[^\w\-\s]', '_', str("%s_R2" % $in2.element_identifier)) + $ext
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40 ln -sf '$in2' '$in2_name' &&
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41 #end if
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42 #end if
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44 ## Run fastp
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45
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46 fastp
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47
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48 --thread \${GALAXY_SLOTS:-1}
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49 --report_title 'fastp report for $in1_name'
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50
5
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51 -i '$in1_name'
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52
12
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53 ## Merge reads
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54
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55 #if str($single_paired.single_paired_selector).startswith('paired')
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56 -I '$in2_name'
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57 #if $single_paired.merge_reads.merge
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58 $single_paired.merge_reads.merge
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59 --merged_out '$merged_reads'
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60 #if $single_paired.merge_reads.include_unmerged
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61 $single_paired.merge_reads.include_unmerged
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62 #else
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63 --out1 '$unmerged_out1'
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64 --out2 '$unmerged_out2'
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65 --unpaired1 '$unpaired1'
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66 --unpaired2 '$unpaired2'
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67 #end if
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68 #else
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69 -o first${ext}
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70 -O second${ext}
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71 #end if
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72 #else
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73 -o first${ext}
0
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74 #end if
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75
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76 ## Adapter Trimming Options
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77
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78 $single_paired.adapter_trimming_options.disable_adapter_trimming
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79
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80 #if str($single_paired.adapter_trimming_options.adapter_sequence1):
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81 --adapter_sequence '$single_paired.adapter_trimming_options.adapter_sequence1'
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82 #end if
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83
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84 #if str($single_paired.single_paired_selector).startswith('paired'):
0
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85 #if str($single_paired.adapter_trimming_options.adapter_sequence2):
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86 --adapter_sequence_r2 '$single_paired.adapter_trimming_options.adapter_sequence2'
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87 #end if
11
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88 $single_paired.adapter_trimming_options.detect_adapter_for_pe
0
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89 #end if
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90
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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91
1
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92 ## Global Trimming Options
0
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93
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94 #if str($single_paired.global_trimming_options.trim_front1):
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95 -f $single_paired.global_trimming_options.trim_front1
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96 #end if
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97
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98 #if str($single_paired.global_trimming_options.trim_tail1):
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99 -t $single_paired.global_trimming_options.trim_tail1
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100 #end if
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101
5
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102 #if str($single_paired.single_paired_selector).startswith('paired'):
0
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103 #if str($single_paired.global_trimming_options.trim_front2):
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104 -F $single_paired.global_trimming_options.trim_front2
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105 #end if
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106 #if str($single_paired.global_trimming_options.trim_tail2):
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107 -T $single_paired.global_trimming_options.trim_tail2
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108 #end if
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109 #end if
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110
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111
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112 ## Overrepresented sequence analysis
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113
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114 $overrepresented_sequence_analysis.overrepresentation_analysis
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115
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116 #if str($overrepresented_sequence_analysis.overrepresentation_sampling):
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117 -P $overrepresented_sequence_analysis.overrepresentation_sampling
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118 #end if
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119
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120
1
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121 ## Filter Options
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122
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123 ## Quality filtering options
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124
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125 $filter_options.quality_filtering_options.disable_quality_filtering
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126
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127 #if str($filter_options.quality_filtering_options.qualified_quality_phred):
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128 -q $filter_options.quality_filtering_options.qualified_quality_phred
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129 #end if
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130 #if str($filter_options.quality_filtering_options.unqualified_percent_limit):
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131 -u $filter_options.quality_filtering_options.unqualified_percent_limit
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132 #end if
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133 #if str($filter_options.quality_filtering_options.n_base_limit):
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134 -n $filter_options.quality_filtering_options.n_base_limit
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135 #end if
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136
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137
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138 ## Length filtering options
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139
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140 $filter_options.length_filtering_options.disable_length_filtering
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141
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142 #if str($filter_options.length_filtering_options.length_required):
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143 -l $filter_options.length_filtering_options.length_required
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144 #end if
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145
9
e9f30696da0e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit a64eb7904f80e90a5faff04ea3fb797ee1e07d36"
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146 #if str($filter_options.length_filtering_options.length_limit):
e9f30696da0e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit a64eb7904f80e90a5faff04ea3fb797ee1e07d36"
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147 --length_limit $filter_options.length_filtering_options.length_limit
e9f30696da0e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit a64eb7904f80e90a5faff04ea3fb797ee1e07d36"
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148 #end if
e9f30696da0e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit a64eb7904f80e90a5faff04ea3fb797ee1e07d36"
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149
1
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150 ## Low complexity filtering options
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151
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152 $filter_options.low_complexity_filter.enable_low_complexity_filter
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153
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154 #if str($filter_options.low_complexity_filter.complexity_threshold):
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155 -Y $filter_options.low_complexity_filter.complexity_threshold
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156 #end if
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157
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158
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159 ## Read Modification Options
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160
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161 ## PolyG tail trimming, useful for NextSeq/NovaSeq data
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162
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163 #if $read_mod_options.polyg_tail_trimming.trimming_select in ['', '-g']:
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164 #if str($read_mod_options.polyg_tail_trimming.poly_g_min_len):
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165 --poly_g_min_len $read_mod_options.polyg_tail_trimming.poly_g_min_len
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166 #end if
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167 $read_mod_options.polyg_tail_trimming.trimming_select
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168 #end if
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169
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170 ## PolyX tail trimming
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171
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172 #if $read_mod_options.polyx_tail_trimming.polyx_trimming_select == '-x':
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173 $read_mod_options.polyx_tail_trimming.polyx_trimming_select
6
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174 #if str($read_mod_options.polyx_tail_trimming.poly_x_min_len):
1
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175 --poly_x_min_len $read_mod_options.polyx_tail_trimming.poly_x_min_len
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176 #end if
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177 #end if
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178
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179 ## UMI processing
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180
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181 #if $read_mod_options.umi_processing.umi:
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182 $read_mod_options.umi_processing.umi
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183 #if str($read_mod_options.umi_processing.umi_loc):
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184 --umi_loc '$read_mod_options.umi_processing.umi_loc'
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185 #end if
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186 #if str($read_mod_options.umi_processing.umi_len):
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187 --umi_len $read_mod_options.umi_processing.umi_len
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188 #end if
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189 #if str($read_mod_options.umi_processing.umi_prefix):
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190 --umi_prefix '$read_mod_options.umi_processing.umi_prefix'
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191 #end if
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192 #end if
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193
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194 ## Per read cutting by quality options
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195
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196 #if $read_mod_options.cutting_by_quality_options.cut_by_quality5 or $read_mod_options.cutting_by_quality_options.cut_by_quality3:
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197
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198 $read_mod_options.cutting_by_quality_options.cut_by_quality5
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199
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200 $read_mod_options.cutting_by_quality_options.cut_by_quality3
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201
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202 #if str($read_mod_options.cutting_by_quality_options.cut_window_size):
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203 -W $read_mod_options.cutting_by_quality_options.cut_window_size
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204 #end if
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205 #if str($read_mod_options.cutting_by_quality_options.cut_mean_quality):
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206 -M $read_mod_options.cutting_by_quality_options.cut_mean_quality
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207 #end if
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208 #end if
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209
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210 ## Base correction by overlap analysis options
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211
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212 $read_mod_options.base_correction_options.correction
0
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213
12
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214 #if str($single_paired.single_paired_selector).startswith('single')
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215 &&
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216 mv first${ext} '${out1}'
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217 #elif str($single_paired.single_paired_selector).startswith('paired') and not $single_paired.merge_reads.merge:
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218 &&
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219 mv first${ext} '${out1}'
0
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220 &&
4
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221 mv second${ext} '${out2}'
0
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222 #end if
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223 ]]></command>
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224 <inputs>
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225 <conditional name="single_paired">
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226 <param name="single_paired_selector" type="select" label="Single-end or paired reads">
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227 <option value="single" selected="true">Single-end</option>
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228 <option value="paired">Paired</option>
5
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229 <option value="paired_collection">Paired Collection</option>
0
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230 </param>
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231 <when value="single">
5
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232 <expand macro="in" />
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233 <expand macro="adapter_trimming_options" />
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234 <expand macro="global_trimming_options" />
0
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235 </when>
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236 <when value="paired">
5
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237 <expand macro="in" read_number="1" argument="-i"/>
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238 <expand macro="in" read_number="2" argument="-I"/>
12
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239 <expand macro="merge_reads" />
5
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240 <expand macro="adapter_trimming_options">
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241 <expand macro="adapter_sequence" read_number="2"/>
11
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242 <expand macro="detect_adapter_for_pe" />
5
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243 </expand>
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244 <expand macro="global_trimming_options_paired" />
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245 </when>
11
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246 <when value="paired_collection">
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247 <param name="paired_input" type="data_collection" format="fastqsanger,fastqsanger.gz" label="Select paired collection(s)" collection_type="paired"/>
12
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248 <expand macro="merge_reads" />
5
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249 <expand macro="adapter_trimming_options">
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250 <expand macro="adapter_sequence" read_number="2"/>
11
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251 <expand macro="detect_adapter_for_pe" />
5
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252 </expand>
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253 <expand macro="global_trimming_options_paired" />
0
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254 </when>
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255 </conditional>
1
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256
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257 <section name="overrepresented_sequence_analysis" title="Overrepresented Sequence Analysis" expanded="False">
0
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258 <param name="overrepresentation_analysis" argument="-p" type="boolean" truevalue="-p" falsevalue="" checked="false" label="Enable overrepresented analysis" help="Enable overrepresented sequence analysis."/>
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259 <param name="overrepresentation_sampling" argument="-P" type="integer" optional="true" label="Overrepresentation sampling" help="One in (--overrepresentation_sampling) reads will be computed for overrepresentation analysis (1~10000), smaller is slower, default is 20."/>
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260 </section>
1
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261
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262 <!-- Filter Options -->
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263 <section name="filter_options" title="Filter Options">
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264 <section name="quality_filtering_options" title="Quality filtering options" expanded="True">
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265 <param name="disable_quality_filtering" argument="-Q" type="boolean" truevalue="-Q" falsevalue="" checked="false" label="Disable quality filtering" help="Quality filtering is enabled by default. If this option is specified, quality filtering is disabled."/>
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266 <param name="qualified_quality_phred" argument="-q" type="integer" optional="true" label="Qualified quality phred" help="The quality value that a base is qualified. Default 15 means phred quality >=Q15 is qualified."/>
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267 <param name="unqualified_percent_limit" argument="-u" type="integer" optional="true" label="Unqualified percent limit" help="How many percents of bases are allowed to be unqualified (0~100). Default 40 means 40%."/>
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268 <param name="n_base_limit" argument="-n" type="integer" optional="true" label="N base limit" help="If one read's number of N base is >n_base_limit, then this read/pair is discarded. Default is 5."/>
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269 </section>
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270
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271 <section name="length_filtering_options" title="Length filtering options" expanded="True">
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272 <param name="disable_length_filtering" argument="-L" type="boolean" truevalue="-L" falsevalue="" checked="false" label="Disable length filtering" help="Length filtering is enabled by default. If this option is specified, length filtering is disabled."/>
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273 <param name="length_required" argument="-l" type="integer" optional="true" label="Length required" help="Reads shorter than this value will be discarded. Default is 15."/>
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274 <param argument="--length_limit" type="integer" optional="true" label="Maximum length" help="Reads longer than this value will be discarded. Default is 0 and means no limitation."/>
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275 </section>
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276
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277 <section name="low_complexity_filter" title="Low complexity filtering options" expanded="True">
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278 <param name="enable_low_complexity_filter" argument="-y" type="boolean" truevalue="-y" falsevalue="" checked="false" label="Enable low complexity filter" help="The complexity is defined as the percentage of base that is different from its next base, default is No"/>
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279 <param name="complexity_threshold" argument="-Y" type="integer" optional="true" label="Complexity threshold" help="Threshold for low complexity filter (0~100). Default is 30, which means 30% complexity is required."/>
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280 </section>
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281 </section>
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282
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283 <!-- Read Modification Options -->
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284 <section name="read_mod_options" title="Read Modification Options">
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285 <conditional name="polyg_tail_trimming">
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286 <param name="trimming_select" type="select" label="PolyG tail trimming" help="This feature is enabled for NextSeq/NovaSeq data by default. NextSeq/NovaSeq data is detected by the machine ID in the FASTQ records.">
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287 <option value="" selected="true">Automatic trimming for Illumina NextSeq/NovaSeq data</option>
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288 <option value="-g">Force polyG tail trimming</option>
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289 <option value="-G">Disable polyG tail trimming</option>
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290 </param>
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291 <when value="-g">
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292 <expand macro="poly_g_min_len" />
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293 </when>
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294 <when value="">
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295 <expand macro="poly_g_min_len" />
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296 </when>
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297 <when value="-G" />
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298 </conditional>
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299
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300 <conditional name="polyx_tail_trimming">
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301 <param name="polyx_trimming_select" type="select" label="PolyX tail trimming" help="Similar to polyG tail trimming. When polyG tail trimming and polyX tail trimming are both enabled, fastp will perform polyG trimming first, then perform polyX trimming. Disabled by default.">
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302 <option value="" selected="true">Disable polyX trimming</option>
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303 <option value="-x">Enable polyX tail trimming</option>
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304 </param>
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305 <when value="-x">
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306 <param argument="--poly_x_min_len" type="integer" optional="true" label="PolyX minimum length"
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307 help="The minimum length to detect polyX in the read tail. 10 by default."/>
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308 </when>
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309 <when value="" />
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310 </conditional>
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311
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312 <section name="umi_processing" title="UMI processing" expanded="True">
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313 <param name="umi" argument="-U" type="boolean" truevalue="-U" falsevalue="" checked="false" label="Enable unique molecular identifer" help="Enable unique molecular identifer (UMI) preprocessing."/>
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314 <param argument="--umi_loc" type="text" optional="true" label="UMI location" help="Specify the location of UMI, can be (index1/index2/read1/read2/per_index/per_read, default is none."/>
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315 <param argument="--umi_len" type="integer" optional="true" label="UMI length" help="If the UMI is in read1/read2, its length should be provided."/>
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316 <param argument="--umi_prefix" type="text" optional="true" label="UMI prefix" help="If specified, an underline will be used to connect prefix and UMI (i.e. prefix=UMI, UMI=AATTCG, final=UMI_AATTCG). No prefix by default."/>
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317 </section>
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318
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319 <section name="cutting_by_quality_options" title="Per read cutting by quality options" expanded="True">
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320 <param name="cut_by_quality5" argument="-5" type="boolean" truevalue="-5" falsevalue="" checked="false" label="Cut by quality in front (5')" help="Enable per read cutting by quality in front (5'), default is disabled (WARNING: this will interfere deduplication for both PE/SE data)."/>
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321 <param name="cut_by_quality3" argument="-3" type="boolean" truevalue="-3" falsevalue="" checked="false" label="Cut by quality in tail (3')" help="Enable per read cutting by quality in tail (3'), default is disabled (WARNING: this will interfere deduplication for SE data)."/>
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322 <param name="cut_window_size" argument="-W" type="integer" optional="true" label="Cutting window size" help="The size of the sliding window for sliding window trimming, default is 4."/>
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323 <param name="cut_mean_quality" argument="-M" type="integer" optional="true" label="Cutting mean quality" help="The bases in the sliding window with mean quality below cutting_quality will be cut, default is Q20."/>
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324 </section>
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325
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326 <section name="base_correction_options" title="Base correction by overlap analysis options" expanded="True">
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327 <param name="correction" argument="-c" type="boolean" truevalue="-c" falsevalue="" checked="false" label="Enable base correction" help="Enable base correction in overlapped regions (only for PE data), default is disabled."/>
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328 </section>
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329 </section>
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330
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331 <section name="output_options" title="Output Options" expanded="False">
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332 <param name="report_html" type="boolean" truevalue="True" falsevalue="False" checked="True" label="Output HTML report" help="fastp provides a QC report for the data Before and After filtering within a single HTML page, which enables comparison of the quality statistics changed by the preprocessing step directly"/>
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333 <param name="report_json" type="boolean" truevalue="True" falsevalue="False" checked="True" label="Output JSON report" help="The JSON report contains all the data visualized in the HTML report. The format of the JSON report is manually optimized to be easily readable by humans and is compatible with MultiQC"/>
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334 </section>
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335 </inputs>
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336
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337 <outputs>
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338 <data name="out1" format_source="in1" label="${tool.name} on ${on_string}: Read 1 output">
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339 <filter>single_paired['single_paired_selector'] in ["single", "paired"] and not single_paired['merge_reads']['merge']</filter>
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340 </data>
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341 <data name="out2" format_source="in2" label="${tool.name} on ${on_string}: Read 2 output">
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342 <filter>single_paired['single_paired_selector'] == "paired" and not single_paired['merge_reads']['merge']</filter>
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343 </data>
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344 <collection name="output_paired_coll" type="paired" format_source="paired_input['forward']" label="${tool.name} on ${on_string}: Paired-end output">
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345 <filter>single_paired['single_paired_selector'] == "paired_collection" and not single_paired['merge_reads']['merge']</filter>
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346 </collection>
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347 <data name="report_html" format="html" from_work_dir="fastp.html" label="${tool.name} on ${on_string}: HTML report">
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348 <filter>output_options['report_html'] is True</filter>
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349 </data>
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350 <data name="report_json" format="json" from_work_dir="fastp.json" label="${tool.name} on ${on_string}: JSON report">
0
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351 <filter>output_options['report_json'] is True</filter>
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352 </data>
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353 <data name="merged_reads" format_source="in1" label="${tool.name} on ${on_string}: Merged reads">
12
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354 <filter>single_paired['single_paired_selector'] in ["paired", "paired_collection"] and single_paired['merge_reads']['merge']</filter>
14
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355 <expand macro="format_actions" read_number="1" forward_reverse="forward" />
12
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356 </data>
14
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357 <data name="unmerged_out1" format_source="in1" label="${tool.name} on ${on_string}: Unmerged filtered reads1">
12
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358 <filter>single_paired['single_paired_selector'] in ["paired", "paired_collection"] and single_paired['merge_reads']['merge'] and not single_paired['merge_reads']['include_unmerged']</filter>
14
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359 <expand macro="format_actions" read_number="1" forward_reverse="forward" />
12
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360 </data>
14
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361 <data name="unmerged_out2" format_source="in2" label="${tool.name} on ${on_string}: Unmerged filtered reads2">
12
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362 <filter>single_paired['single_paired_selector'] in ["paired", "paired_collection"] and single_paired['merge_reads']['merge'] and not single_paired['merge_reads']['include_unmerged']</filter>
14
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363 <expand macro="format_actions" read_number="2" forward_reverse="reverse" />
12
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364 </data>
14
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365 <data name="unpaired1" format_source="in1" label="${tool.name} on ${on_string}: Unmerged unfiltered reads1">
12
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366 <filter>single_paired['single_paired_selector'] in ["paired", "paired_collection"] and single_paired['merge_reads']['merge'] and not single_paired['merge_reads']['include_unmerged']</filter>
14
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367 <expand macro="format_actions" read_number="1" forward_reverse="forward" />
12
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368 </data>
14
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369 <data name="unpaired2" format_source="in2" label="${tool.name} on ${on_string}: Unmerged unfiltered reads2">
12
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370 <filter>single_paired['single_paired_selector'] in ["paired", "paired_collection"] and single_paired['merge_reads']['merge'] and not single_paired['merge_reads']['include_unmerged']</filter>
14
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371 <expand macro="format_actions" read_number="2" forward_reverse="reverse" />
12
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372 </data>
0
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373 </outputs>
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374
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375 <tests>
11
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376 <!-- 1. Ensure default output works -->
12
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377 <test expect_num_outputs="3">
1
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378 <param name="in1" ftype="fastqsanger" value="R1.fq"/>
0
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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379 <param name="single_paired_selector" value="single"/>
1
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380 <output name="out1" ftype="fastqsanger" file="out1.fq"/>
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381 <output name="report_html">
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382 <assert_contents>
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383 <has_text text="fastp report"/>
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384 </assert_contents>
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385 </output>
12
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386 <output name="report_json">
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387 <assert_contents>
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388 <has_text text="fastp report"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
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389 </assert_contents>
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390 </output>
0
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391 </test>
11
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392 <!-- 2. Ensure paired collection works -->
7
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393 <test expect_num_outputs="4">
5
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394 <param name="single_paired_selector" value="paired_collection"/>
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395 <param name="paired_input">
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396 <collection type="paired">
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397 <element name="forward" value="bwa-mem-fastq1.fq" ftype="fastqsanger" />
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398 <element name="reverse" value="bwa-mem-fastq2.fq" ftype="fastqsanger" />
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399 </collection>
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400 </param>
12
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401 <param name="report_json" value="False" />
5
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402 <output name="report_html">
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403 <assert_contents>
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iuc
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404 <has_text text="fastp report"/>
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405 </assert_contents>
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406 </output>
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407 <output_collection name="output_paired_coll" type="paired">
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408 <element name="forward" value="out_bwa1.fq" ftype="fastqsanger"/>
4c14c2f8f4fb planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
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409 <element name="reverse" value="out_bwa2.fq" ftype="fastqsanger"/>
4c14c2f8f4fb planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
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410 </output_collection>
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411 </test>
11
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412 <!-- 3. Ensure custom adapter works -->
1
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413 <test expect_num_outputs="2">
12
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414 <param name="in1" ftype="fastqsanger" value="R1.fq"/>
0
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415 <param name="single_paired_selector" value="single"/>
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416 <param name="adapter_sequence1" value="ATCG"/>
12
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417 <param name="report_json" value="False" />
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418 <output name="out1" ftype="fastqsanger" file="out_a.fq"/>
0
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419 </test>
11
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420 <!-- 4. Ensure UMI processing works -->
1
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421 <test expect_num_outputs="2">
12
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422 <param name="in1" ftype="fastqsanger" value="R1.fq"/>
0
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423 <param name="single_paired_selector" value="single"/>
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424 <section name="umi_processing">
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425 <param name="umi" value="true"/>
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426 <param name="umi_loc" value="read1"/>
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427 <param name="umi_len" value="8"/>
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428 </section>
12
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429 <param name="report_json" value="False" />
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430 <output name="out1" ftype="fastqsanger" file="out2.fq"/>
0
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431 </test>
11
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432 <!-- 5. Ensure UMI processing with different lengths works -->
1
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433 <test expect_num_outputs="2">
12
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434 <param name="in1" ftype="fastqsanger" value="R1.fq"/>
0
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435 <param name="single_paired_selector" value="single"/>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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parents:
diff changeset
436 <section name="umi_processing">
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
437 <param name="umi" value="true"/>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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diff changeset
438 <param name="umi_loc" value="read1"/>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
439 <param name="umi_len" value="12"/>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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440 </section>
12
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parents: 11
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441 <param name="report_json" value="False" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
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442 <output name="out1" ftype="fastqsanger" file="out3.fq"/>
0
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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443 </test>
12
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iuc
parents: 11
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444 <!-- 6. Ensure paired-end fastqsanger works -->
1
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iuc
parents: 0
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445 <test expect_num_outputs="3">
12
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iuc
parents: 11
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446 <param name="in1" ftype="fastqsanger" value="bwa-mem-fastq1.fq"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
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447 <param name="in2" ftype="fastqsanger" value="bwa-mem-fastq2.fq"/>
0
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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448 <param name="single_paired_selector" value="paired"/>
12
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iuc
parents: 11
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449 <param name="report_json" value="False" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
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450 <output name="out1" ftype="fastqsanger" file="out_bwa1.fq"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
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451 <output name="out2" ftype="fastqsanger" file="out_bwa2.fq"/>
0
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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452 </test>
11
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parents: 9
diff changeset
453 <!-- 7. Ensure paired-end UMI processing of Read 1 works -->
1
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iuc
parents: 0
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454 <test expect_num_outputs="3">
12
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iuc
parents: 11
diff changeset
455 <param name="in1" ftype="fastqsanger" value="bwa-mem-fastq1.fq"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
456 <param name="in2" ftype="fastqsanger" value="bwa-mem-fastq2.fq"/>
0
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
457 <param name="single_paired_selector" value="paired"/>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
458 <section name="umi_processing">
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
459 <param name="umi" value="true"/>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
460 <param name="umi_loc" value="read1"/>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
461 <param name="umi_len" value="8"/>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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diff changeset
462 </section>
12
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iuc
parents: 11
diff changeset
463 <param name="report_json" value="False" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
464 <output name="out1" ftype="fastqsanger" file="out_bwa_umi_read1_1.fq"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
465 <output name="out2" ftype="fastqsanger" file="out_bwa_umi_read1_2.fq"/>
0
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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466 </test>
11
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iuc
parents: 9
diff changeset
467 <!-- 8. Ensure paired-end UMI processing of Read 2 works -->
12
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
468 <test expect_num_outputs="4">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
469 <param name="in1" ftype="fastqsanger" value="bwa-mem-fastq1.fq"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
470 <param name="in2" ftype="fastqsanger" value="bwa-mem-fastq2.fq"/>
0
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
471 <param name="single_paired_selector" value="paired"/>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
472 <section name="umi_processing">
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
473 <param name="umi" value="true"/>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
474 <param name="umi_loc" value="read2"/>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
475 <param name="umi_len" value="8"/>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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diff changeset
476 </section>
12
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iuc
parents: 11
diff changeset
477 <output name="out1" ftype="fastqsanger" file="out_bwa_umi_read2_1.fq"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
478 <output name="out2" ftype="fastqsanger" file="out_bwa_umi_read2_2.fq"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
479 <output name="report_json">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
480 <assert_contents>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
481 <has_text text="fastp report"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
482 </assert_contents>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
483 </output>
0
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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484 </test>
11
d5ae828d1c3c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6b8fbd9db12329e2f49eee672b185015c3e35d4f
iuc
parents: 9
diff changeset
485 <!-- 9. Ensure JSON report output works -->
1
adf6b091b0a8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents: 0
diff changeset
486 <test expect_num_outputs="2">
adf6b091b0a8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents: 0
diff changeset
487 <param name="in1" ftype="fastqsanger" value="R1.fq"/>
0
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
488 <param name="single_paired_selector" value="single"/>
1
adf6b091b0a8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents: 0
diff changeset
489 <param name="report_html" value="False"/>
adf6b091b0a8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents: 0
diff changeset
490 <output name="out1" ftype="fastqsanger" file="out1.fq"/>
adf6b091b0a8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents: 0
diff changeset
491 <output name="report_json">
0
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iuc
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diff changeset
492 <assert_contents>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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diff changeset
493 <has_text text="fastp report"/>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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494 </assert_contents>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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diff changeset
495 </output>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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496 </test>
11
d5ae828d1c3c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6b8fbd9db12329e2f49eee672b185015c3e35d4f
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parents: 9
diff changeset
497 <!-- 10. Ensure polyG trimming works -->
12
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iuc
parents: 11
diff changeset
498 <test expect_num_outputs="3">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
499 <param name="in1" ftype="fastqsanger.gz" value="R1.fq.gz"/>
0
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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diff changeset
500 <param name="single_paired_selector" value="single"/>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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diff changeset
501 <param name="trimming_select" value="-g"/>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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diff changeset
502 <param name="poly_g_min_len" value="10"/>
12
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iuc
parents: 11
diff changeset
503 <output name="out1" ftype="fastqsanger.gz" decompress="True" file="out1.fq.gz"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
504 <output name="report_json">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
505 <assert_contents>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
506 <has_text text="fastp report"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
507 </assert_contents>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
508 </output>
0
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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diff changeset
509 </test>
11
d5ae828d1c3c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6b8fbd9db12329e2f49eee672b185015c3e35d4f
iuc
parents: 9
diff changeset
510 <!-- 11. Ensure polyX trimming works -->
12
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
511 <test expect_num_outputs="3">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
512 <param name="in1" ftype="fastqsanger.gz" value="R1.fq.gz"/>
6
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iuc
parents: 5
diff changeset
513 <param name="single_paired_selector" value="single"/>
7c49e331a737 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 72f3e06941d063dffce10741fcebea38a536ee15
iuc
parents: 5
diff changeset
514 <param name="trimming_select" value="-G"/>
7c49e331a737 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 72f3e06941d063dffce10741fcebea38a536ee15
iuc
parents: 5
diff changeset
515 <param name="polyx_trimming_select" value="-x"/>
7c49e331a737 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 72f3e06941d063dffce10741fcebea38a536ee15
iuc
parents: 5
diff changeset
516 <param name="poly_x_min_len" value="10"/>
12
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iuc
parents: 11
diff changeset
517 <output name="out1" ftype="fastqsanger.gz" decompress="True" file="out1.fq.gz"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
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diff changeset
518 <output name="report_json">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
519 <assert_contents>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
520 <has_text text="fastp report"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
521 </assert_contents>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
522 </output>
6
7c49e331a737 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 72f3e06941d063dffce10741fcebea38a536ee15
iuc
parents: 5
diff changeset
523 </test>
12
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
524 <!-- 12. Test fastqsanger files with different length -->
11
d5ae828d1c3c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6b8fbd9db12329e2f49eee672b185015c3e35d4f
iuc
parents: 9
diff changeset
525 <test expect_exit_code="255" expect_failure="true">
d5ae828d1c3c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6b8fbd9db12329e2f49eee672b185015c3e35d4f
iuc
parents: 9
diff changeset
526 <param name="single_paired_selector" value="paired_collection"/>
d5ae828d1c3c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6b8fbd9db12329e2f49eee672b185015c3e35d4f
iuc
parents: 9
diff changeset
527 <param name="paired_input">
d5ae828d1c3c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6b8fbd9db12329e2f49eee672b185015c3e35d4f
iuc
parents: 9
diff changeset
528 <collection type="paired">
d5ae828d1c3c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6b8fbd9db12329e2f49eee672b185015c3e35d4f
iuc
parents: 9
diff changeset
529 <element name="forward" value="bwa-mem-fastq1.fq" ftype="fastqsanger" />
d5ae828d1c3c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6b8fbd9db12329e2f49eee672b185015c3e35d4f
iuc
parents: 9
diff changeset
530 <element name="reverse" value="bwa-mem-fastq2_too_long.fq" ftype="fastqsanger" />
d5ae828d1c3c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6b8fbd9db12329e2f49eee672b185015c3e35d4f
iuc
parents: 9
diff changeset
531 </collection>
d5ae828d1c3c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6b8fbd9db12329e2f49eee672b185015c3e35d4f
iuc
parents: 9
diff changeset
532 </param>
d5ae828d1c3c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6b8fbd9db12329e2f49eee672b185015c3e35d4f
iuc
parents: 9
diff changeset
533 </test>
12
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
534 <!-- 13. Test merge reads in combination with paired -->
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
535 <test expect_num_outputs="5">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
536 <param name="in1" ftype="fastqsanger" value="bwa-mem-fastq1.fq"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
537 <param name="in2" ftype="fastqsanger" value="bwa-mem-fastq2.fq"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
538 <param name="single_paired_selector" value="paired"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
539 <param name="merge" value="--merge" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
540 <param name="report_html" value="False" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
541 <param name="report_json" value="False" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
542 <output name="merged_reads" ftype="fastqsanger" file="bwa-mem-merged-reads.fastqsanger" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
543 <output name="unmerged_out1" ftype="fastqsanger" file="bwa-mem-unmerged-filtered-reads1.fastqsanger" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
544 <output name="unmerged_out2" ftype="fastqsanger" file="bwa-mem-unmerged-filtered-reads2.fastqsanger" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
545 <output name="unpaired1" ftype="fastqsanger" file="bwa-mem-unmerged-unfiltered-reads1.fastqsanger" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
546 <output name="unpaired2" ftype="fastqsanger">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
547 <assert_contents>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
548 <has_size size="0" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
549 </assert_contents>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
550 </output>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
551 </test>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
552 <!-- 14. Test merge and include_unmerged in combination with paired collection -->
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
553 <test expect_num_outputs="2">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
554 <param name="single_paired_selector" value="paired_collection"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
555 <param name="paired_input">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
556 <collection type="paired">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
557 <element name="forward" value="bwa-mem-fastq1.fq" ftype="fastqsanger" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
558 <element name="reverse" value="bwa-mem-fastq2.fq" ftype="fastqsanger" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
559 </collection>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
560 </param>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
561 <param name="merge" value="--merge" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
562 <param name="include_unmerged" value="--include_unmerged" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
563 <param name="report_html" value="False" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
564 <output name="merged_reads" ftype="fastqsanger" file="bwa-mem-merged-read-include-unmerged.fastqsanger" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
565 <output name="report_json">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
566 <assert_contents>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
567 <has_text text="fastp report"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
568 </assert_contents>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
569 </output>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
570 </test>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
571 <!--15. Test paired collection in combination with compressed input-->
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
572 <test expect_num_outputs="4">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
573 <param name="single_paired_selector" value="paired_collection"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
574 <param name="paired_input">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
575 <collection type="paired">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
576 <element name="forward" value="bwa-mem-fastq-paired-collection/input_forward.fastqsanger.gz" ftype="fastqsanger.gz" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
577 <element name="reverse" value="bwa-mem-fastq-paired-collection/input_reverse.fastqsanger.gz" ftype="fastqsanger.gz" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
578 </collection>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
579 </param>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
580 <param name="report_json" value="False" />
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
581 <output name="report_html">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
582 <assert_contents>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
583 <has_text text="fastp report"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
584 </assert_contents>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
585 </output>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
586 <output_collection name="output_paired_coll" type="paired">
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
587 <element name="forward" value="bwa-mem-fastq-paired-collection/output_forward.fastqsanger.gz" decompress="True" ftype="fastqsanger.gz"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
588 <element name="reverse" value="bwa-mem-fastq-paired-collection/output_reverse.fastqsanger.gz" decompress="True" ftype="fastqsanger.gz"/>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
589 </output_collection>
6292d4ff1ddd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 78e6d7efcb74d3baa4e5611a7be4ad82b51bfe0d
iuc
parents: 11
diff changeset
590 </test>
0
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
591 </tests>
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
592 <help><![CDATA[
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
593 .. class:: infomark
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
594
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
595 **What it does**
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
596
11
d5ae828d1c3c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6b8fbd9db12329e2f49eee672b185015c3e35d4f
iuc
parents: 9
diff changeset
597 fastp_ is a tool designed to provide fast all-in-one preprocessing for FASTQ files. This tool is developed in C++ with multithreading supported to
d5ae828d1c3c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6b8fbd9db12329e2f49eee672b185015c3e35d4f
iuc
parents: 9
diff changeset
598 afford high performance.
0
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
599
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
600 *Features*
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
601
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
602 1. Filter out bad reads (too low quality, too short, or too many N...)
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff changeset
603
1
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604 2. Cut low quality bases for per read in its 5' and 3' by evaluating the mean quality from a sliding window (like Trimmomatic but faster)
0
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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605
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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606 3. Trim all reads in front and tail
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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607
1
adf6b091b0a8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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608 4. Cut adapters. Adapter sequences can be automatically detected, which means you don't have to input the adapter sequences to trim them.
adf6b091b0a8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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609
adf6b091b0a8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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610 5. Correct mismatched base pairs in overlapped regions of paired end reads, if one base is with high quality while the other is with ultra-low quality
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cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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611
1
adf6b091b0a8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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612 6. Trim polyG in 3' ends, which is commonly seen in NovaSeq/NextSeq data. Trim polyX in 3' ends to remove unwanted polyX tailing (i.e. polyA tailing for mRNA-Seq data)
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cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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613
12
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614 7. Preprocess unique molecular identifier (UMI) enabled data, shift UMI to sequence name
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cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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615
1
adf6b091b0a8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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616 8. Report JSON format result for further interpreting
0
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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617
1
adf6b091b0a8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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618 9. Visualize quality control and filtering results on a single HTML page (like FASTQC but faster and more informative)
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cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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619
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620 10. Split the output to multiple files (0001.R1.gz, 0002.R1.gz...) to support parallel processing. Two modes can be used, limiting the total split file number, or limiting the lines of each split file (*Not enabled in this Galaxy tool*)
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cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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621
1
adf6b091b0a8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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622 11. Support long reads (data from PacBio / Nanopore devices)
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cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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623
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624 -----
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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625
cb7226f7c585 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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626 **Inputs**
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627
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628 Single-end or Paired-end (compressed) fastqsanger files
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629
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630 -----
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631
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632 **Outputs**
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633
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634 * Processed reads
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635 * Merged reads
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636 * Unmerged filtered reads1, reads that cannot be merged successfully, but both pass all the filters.
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637 * Unmerged filtered reads2, reads that cannot be merged successfully, but both pass all the filters.
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638 * Unmerged unfiltered reads1, reads that cannot be merged, **read1** passes filters but **read2** doesn't.
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639 * Unmerged unfiltered reads2, reads that cannot be merged, **read2** passes filters but **read1** doesn't.
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640
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641 Optionally, under **Output Options** you can choose to output
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642
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643 * HTML report (default is Yes)
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644 * JSON report (compatible with MultiQC)
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645
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646 .. _fastp: https://github.com/OpenGene/fastp
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647
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648 ]]></help>
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649 <citations>
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650 <citation type="doi">10.1101/274100</citation>
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651 </citations>
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652 </tool>