Mercurial > repos > bgruening > deeptools_plot_correlation

annotate plotCorrelation.xml @ 2:c5634baf9bf9 draft

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author bgruening
date Fri, 18 Dec 2015 12:27:26 -0500
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1 <tool id="deeptools_plot_correlation" name="plotCorrelation" version="@WRAPPER_VERSION@.0">
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2 <description>creates a heatmap of correlation scores between different samples </description>
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3 <macros>
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4 <token name="@BINARY@">plotCorrelation</token>
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5 <import>deepTools_macros.xml</import>
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6 </macros>
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7 <expand macro="requirements"/>
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8 <command>
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9 <![CDATA[
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10 @BINARY@
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11 --corData "$corData"
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12 --plotFile "$outFileName"
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13 --corMethod "$corMethod"
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14 --whatToPlot "$whatToPlot"
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15 $skipZeros
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16 --plotFileFormat "$outFileFormat"
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17 $removeOutliers
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18 --outFileCorMatrix "$matrix"
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19 @HEATMAP_OPTIONS@
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21 ]]>
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22 </command>
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23 <inputs>
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24 <param name="corData" format="deeptools_coverage_matrix" type="data" label="Matrix file from the bamCorrelate tool"/>
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25 <expand macro="corMethod" />
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27 <param argument="--whatToPlot" type="select" label="Plotting type">
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28 <option value="heatmap" selected="True">Heatmap</option>
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29 <option value="scatterplot">Scatterplot</option>
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30 </param>
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31
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32 <expand macro="skipZeros" />
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33
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34 <expand macro="image_file_format" />
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35
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36 <param argument="--removeOutliers" type="boolean"
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37 truevalue="--removeOutliers" falsevalue="" label="Remove regions with very large counts"
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38 help="If set, bins with very large counts are removed. Bins
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39 with abnormally high reads counts artificially
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40 increase pearson correlation; that's why, by default,
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41 bamCorrelate tries to remove outliers using the median
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42 absolute deviation (MAD) method applying a threshold
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43 of 200 to only consider extremely large deviations
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44 from the median. ENCODE blacklist page (https://sites.
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45 google.com/site/anshulkundaje/projects/blacklists)
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46 contains useful information about regions with
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47 unusually high counts."/>
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48
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49 <param name="outFileCorMatrix" type="boolean" label="Save the matrix of values underlying the heatmap"/>
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50 <expand macro="heatmap_options" />
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51
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52 </inputs>
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53 <outputs>
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54 <expand macro="output_image_file_format" />
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55 <data format="tabular" name="matrix" label="${tool.name} on ${on_string}: Correlation matrix">
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56 <filter>outFileCorMatrix is True</filter>
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57 </data>
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58 </outputs>
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59 <tests>
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60 <test>
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61 <param name="corData" value="bamCorrelate_result1.npz" ftype="deeptools_coverage_matrix" />
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62 <param name="outFileFormat" value="png" />
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63 <param name="outFileCorMatrix" value="True" />
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64 <output name="matrix" file="plotCorrelation_result1.tabular" ftype="tabular" />
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65 <output name="outFileName" file="plotCorrelation_result1.png" ftype="png" compare="sim_size" delta="100" />
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66 </test>
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67 <test>
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68 <param name="corData" value="bamCorrelate_result1.npz" ftype="deeptools_coverage_matrix" />
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69 <param name="outFileFormat" value="png" />
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70 <param name="whatToPlot" value="scatterplot" />
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71 <param name="removeOutliers" value="True" />
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72 <param name="plotTitle" value="Test Plot" />
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73 <output name="outFileName" file="plotCorrelation_result2.png" compare="sim_size" delta="100" />
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74 </test>
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75 </tests>
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76 <help>
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77 <![CDATA[
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78 **What it does**
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79
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80 This tools takes a compressed matrix of scores (such as read coverages) for a number of genomic regions
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81 and different samples. It can visualize the correlation among samples as scatterplots or as
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82 heatmap of correlation coefficients. Further output files are optional.
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83 The compressed input matrices are easily generated using the "bamCorrelate" and "bigwigCorrelate" modules of deeptools.
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84
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85
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86 .. image:: $PATH_TO_IMAGES/QC_bamCorrelate_humanSamples.png
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87 :alt: Heatmap of RNA Polymerase II ChIP-seq
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88
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89
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90 You can find more details on plotCorrelation here http://deeptools.readthedocs.org/en/release-1.6/content/tools/plotCorrelation.html
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91
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92
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93 **Output files**:
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94
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95 - **correlation structure**: a scatterplot of all mutual correlations between all samples in matrix
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96 - **diagnostic plot**: clustered heatmap displaying the values for each pair-wise correlation, see below for an example
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97 - data matrix (optional): if you want to analyze or plot the correlation values using a different program, e.g. R, this matrix can be used
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98
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99 -----
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100
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101 @REFERENCES@
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102 ]]>
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103 </help>
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104 <expand macro="citations" />
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105 </tool>