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1 <tool id="star_fusion" name="Star Fusion" version="1.0.0" profile="17.05">
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2 <description>Fusion-finding Pipeline Using the STAR Aligner</description>
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3 <requirements>
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4 <requirement type="package" version="1.1.0">star-fusion</requirement>
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10
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5 <requirement type="package" version="1.3">samtools</requirement>
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8
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6 </requirements>
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7 <command detect_errors="default">
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8 <![CDATA[
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9 STAR-Fusion
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10 --genome_lib_dir "${genome_ref_lib.fields.path}"
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9
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11 --left_fq "${left_input}"
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12 --right_fq "${right_input}"
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13 --output_dir subdir
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14 ]]>
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15 </command>
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16 <stdio>
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17 <exit_code range="1:" level="fatal" description="Error returned from pipeline" />
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18 </stdio>
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19 <regex match="Must investigate error above."
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20 source="stderr"
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21 level="fatal"
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22 description="Unknown error encountered" />
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23 <inputs>
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24 <param format="fastq" name="left_input" type="data" label="Left/Forward strand reads" help=""/>
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25 <param format="fastq" name="right_input" type="data" label="Right/Reverse strand reads" help=""/>
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26 <param name="genome_ref_lib" type="select" label="Select a reference genome">
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27 <options from_data_table="ctat_genome_ref_libs">
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28 <filter type="sort_by" column="2" />
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29 <validator type="no_options" message="No indexes are available" />
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30 </options>
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31 </param>
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32
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33 </inputs>
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34 <outputs>
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35 <data format="txt" name="starfusion_log" label="${tool.name} on ${on_string}: log" from_work_dir="subdir/Log.out"/>
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36 <data format="bam" name="aligned_bam" label="${tool.name} on ${on_string}: Aligned Bam" from_work_dir="subdir/Aligned.sortedByCoord.out.bam"/>
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37 <data format="tabular" name="candidates" label="${tool.name} on ${on_string}: Fusion candidates" from_work_dir="subdir/star-fusion.fusion_candidates.final.abridged"/>
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38 <data format="tabular" name="chimeric_junc" label="${tool.name} on ${on_string}: Chimeric.out.junction" from_work_dir="subdir/Chimeric.out.junction"/>
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39 </outputs>
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40 <tests>
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41 <test>
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42 <param name="left_input" value="StarFusion/reads_1.fq.gz" />
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43 <param name="right_input" value="StarFusion/reads_2.fq.gz" />
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44 <!--
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45 <param name="left_input" value="reads.left.simPE.fq" />
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46 <param name="right_input" value="reads.right.simPE.fq" />
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47 -->
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48 <!--
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49 <output name="aligned_bam" file="SF_out_aligned.bam" />
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50 <output name="candidates" file="SF_out_fusion_candidates.dat" />
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51 <output name="chimeric_junc" file="SF_out_chimeric.junction" />
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52 <output name="starfusion_log" file="SF_out.log" />
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53 -->
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54 <output name="starfusion_log">
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55 <assert_contents>
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56 <has_line_matching expression=".+" />
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57 <has_line line="ALL DONE!" />
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58 </assert_contents>
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59 </output>
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60 <output name="aligned_bam">
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61 <assert_contents>
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62 <has_line_matching expression=".+" />
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63 <!-- The following checks for the magic number at the start of the bam file -->
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64 <!-- At first I thought "\x8B\x1F" was the number, but it turns out the file
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65 produced by the StarFusion test had that sequence in the file somewhere else.
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66 -->
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67 <has_text_matching expression="\x1F\x8B" />
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68 </assert_contents>
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69 </output>
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70 <output name="candidates">
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71 <assert_contents>
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72 <has_line_matching expression=".+" />
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73 <has_line line="#fusion_name	JunctionReads	SpanningFrags	Splice_type	LeftGene	LeftBreakpoint	RightGene	RightBreakpoint	Long_double_anchor_support" />
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74 </assert_contents>
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75 </output>
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76 <output name="chimeric_junc">
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77 <assert_contents>
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78 <has_line_matching expression=".+" />
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79 <has_line_matching expression="^chr20.*" />
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80 </assert_contents>
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81 </output>
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82 </test>
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83 </tests>
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84 <help>
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85 .. class:: infomark
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86
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87 STAR-Fusion is a component of the Trinity Cancer Transcriptome Analysis Toolkit (CTAT). STAR-Fusion uses the STAR aligner to identify candidate fusion transcripts supported by Illumina reads. STAR-Fusion further processes the output generated by the STAR aligner to map junction reads and spanning reads to a reference annotation set. Please read more here_.
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88
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89 .. _here: https://github.com/STAR-Fusion/STAR-Fusion/wiki
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90
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91 </help>
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92 </tool>
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