Mercurial > repos > trinity_ctat > ctat_edger_differential_expression
annotate ctat_edger_differential_expression.xml @ 1:57907c40385c draft default tip
Updating tools requirements.
author | trinity_ctat |
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date | Wed, 27 Jun 2018 14:12:38 -0400 |
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1 <tool id="ctat_edger_differential_expression" name="ctat_edger_differential_expression" version="1.0.0" profile="17.05"> |
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2 |
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3 <description>Identify Differentially Expressed Transcripts Using EdgeR</description> |
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4 <requirements> |
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5 <requirement type="package" version="2.7">python</requirement> |
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6 <requirement type="package">subprocess32</requirement> |
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7 <requirement type="package">bzip2</requirement> |
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8 <requirement type="package" version="1.3.0">rsem</requirement> |
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9 <requirement type="package" version="3">bioconductor-edger</requirement> |
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10 <requirement type="package" version="2">bioconductor-qvalue</requirement> |
1 | 11 <requirement type="package" version="2.6.6">trinity</requirement> |
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12 </requirements> |
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13 <command detect_errors="exit_code"> |
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14 <![CDATA[ |
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15 python $__tool_directory__/ctat_edger_differential_expression.py |
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16 $counts_matrix |
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17 $dispersion |
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18 ]]> |
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19 </command> |
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20 <inputs> |
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21 |
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22 <param type="data" format="txt" name="counts_matrix" label="Matrix of RNA-Seq fragment counts for transcripts per condition" /> |
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23 <param type="data" format="fasta" name="transcripts_fasta_file" label="Transcripts fasta file corresponding to matrix" /> |
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24 <param type="float" name="dispersion" value="0.1" min="0" label="dispersion value" help="Dispersion value to be used in the negative binomial" /> |
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25 |
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26 </inputs> |
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27 <outputs> |
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28 <!-- |
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29 <data format="tar.gz" name="EdgeR_Archive" label="${tool.name} on ${on_string}: EdgeR_Results.tar.gz" from_work_dir="edgeR_results.tar.gz" /> |
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30 --> |
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31 <data format="txt" name="EdgeR_Archive" label="${tool.name} on ${on_string}: EdgeR_Results.tar.gz" from_work_dir="edgeR_results.tar.gz" /> |
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32 |
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33 </outputs> |
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34 <tests> |
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35 <test> |
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36 <param name="counts_matrix" value="Sp.counts.matrix" /> |
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37 <!-- The transcripts_fasta_file does not seem to be used for anything. --> |
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38 <param name="transcripts_fasta_file" value="Sp.Trinity.fasta" /> |
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39 <param name="dispersion" value="0.1" /> |
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40 |
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41 <!-- One could create more detailed tests if the output files were explicitly |
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42 saved rather than placed into an archive. We had a case where the archive |
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43 was being created, but it was missing one of the files, or one of the |
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44 files was empty. There is no easy way to look into the archive file to |
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45 test this. |
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46 --> |
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47 <output name="EdgeR_Archive" > |
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48 <assert_contents> |
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49 <has_line_matching expression=".+" /> |
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50 <!-- The following is the magic number for all gzip files. --> |
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51 <has_text_matching expression="\x1F\x8B" /> |
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52 </assert_contents> |
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53 </output> |
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54 </test> |
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55 </tests> |
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56 <help> |
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57 .. class:: infomark |
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58 |
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59 edgeR is a Bioconductor package focusing on the analysis of digital gene expression data derived from RNA-Seq sequencing technologies. |
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60 |
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61 To learn more about edgeR read their paper_, visit their website_ , or read this user_ guide_ . |
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62 If you are following the Trinity RNA-seq protocol please go here_ for a galaxy tool walk through or the Nature Protocols publication_ . |
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63 |
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64 .. _paper: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2796818/ |
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65 .. _publication: http://www.nature.com/nprot/journal/v8/n8/full/nprot.2013.084.html |
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66 .. _user: https://bioconductor.org/packages/devel/bioc/vignettes/edgeR/inst/doc/edgeRUsersGuide.pdf |
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67 .. _guide: https://bioconductor.org/packages/devel/bioc/vignettes/edgeR/inst/doc/edgeRUsersGuide.pdf |
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68 .. _website: http://bioinf.wehi.edu.au/edgeR/ |
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69 .. _here: https://github.com/trinityrnaseq/GalaxyTrinityProtocol/wiki |
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70 </help> |
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71 |
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72 <citations> |
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73 <citation type="doi">10.1038/nbt.1883</citation> |
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74 </citations> |
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75 |
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76 </tool> |