Mercurial > repos > rnateam > sortmerna

annotate sortmerna.xml @ 5:233da82dc31c draft

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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/sortmerna commit 4febb4056eb7b379edc0f3e7719a50e575a749c8
author rnateam
date Wed, 09 Dec 2015 09:21:28 -0500
parents e6727cef3083
children 97f73e0566c9
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233da82dc31c planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/sortmerna commit 4febb4056eb7b379edc0f3e7719a50e575a749c8
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1 <tool id="bg_sortmerna" name="Filter with SortMeRNA" version="2.1.0">
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2 <description>Fast and accurate filtering of ribosomal RNAs in metatranscriptomic data</description>
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3 <requirements>
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4 <requirement type='package' version="2.0">sortmerna</requirement>
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5 </requirements>
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6 <stdio>
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7 <regex match="This program builds a Burst trie on an input rRNA database"
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8 source="both"
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9 level="fatal"
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10 description="Buildtrie program failed to execute." />
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11 <regex match="The database name"
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12 source="both"
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13 level="fatal"
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14 description="The database ${databases} has not been preprocessed using buildtrie before using SortMeRNA." />
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15 </stdio>
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16 <version_command>
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17 <![CDATA[
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18 sortmerna --version 2>&1|grep 'SortMeRNA version'
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19 ]]>
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20 </version_command>
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21 <command>
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22 <![CDATA[
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23 #set $ref = ''
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24 #set $sep=''
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25 #if str( $databases_type.databases_selector ) == 'history'
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26 #for $db in $databases_type.database_name
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27 #set $ref += $sep + str($db) + ',' + $os.path.splitext($os.path.basename(str($db)))[0]
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28 #set $sep = ':'
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29 #end for
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30 #else if str( $databases_type.databases_selector ) == 'cached_to_index'
233da82dc31c planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/sortmerna commit 4febb4056eb7b379edc0f3e7719a50e575a749c8
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31 ## databases path is not directly accessible, must match by hand with LOC file contents
233da82dc31c planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/sortmerna commit 4febb4056eb7b379edc0f3e7719a50e575a749c8
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32 #set $data_table = dict([(_[0], _[2]) for _ in $databases_type.input_databases.input.options.tool_data_table.data])
233da82dc31c planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/sortmerna commit 4febb4056eb7b379edc0f3e7719a50e575a749c8
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33 #for $db in $databases_type.input_databases.value
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34 #set $ref += $sep + $data_table[$db] + ',' + $os.path.splitext($data_table[$db])[0] + '-reindexed'
233da82dc31c planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/sortmerna commit 4febb4056eb7b379edc0f3e7719a50e575a749c8
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35 #set $sep = ':'
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36 #end for
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37 #else:
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38 ## databases path is not directly accessible, must match by hand with LOC file contents
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39 #set $data_table = dict([(_[0], _[2]) for _ in $databases_type.input_databases.input.options.tool_data_table.data])
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40 #for $db in $databases_type.input_databases.value
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41 #set $ref += $sep + $data_table[$db] + ',' + $os.path.splitext($data_table[$db])[0]
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42 #set $sep = ':'
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43 #end for
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44 #end if
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46 #if str( $databases_type.databases_selector ) != 'cached':
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47 indexdb_rna
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48 --ref $ref
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49 -L $databases_type.seed_length
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50 --max_pos $databases_type.max_pos
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51 &&
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52 #end if
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54 sortmerna
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55 --ref $ref
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56 --reads $input_reads
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57 --aligned aligned
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59 #if str( $sequencing_type.sequencing_type_selector ) == 'paired'
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60 $sequencing_type.paired_type
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61 #end if
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63 $strand_search
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64 $aligned_fastx.aligned_fastx_selector
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65 #if $aligned_fastx.aligned_fastx_selector == '--fastx'
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66 #if $aligned_fastx.other
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67 --other other_file
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68 #end if
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69 #end if
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70 $aligned_sam.aligned_sam_selector
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71 #if $aligned_sam.aligned_sam_selector == '--sam'
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72 $aligned_sam.sq
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73 #end if
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74 $aligned_blast
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75
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76 $log
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77
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78 #if $report.report_type == 'best'
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79 #if $report.report_best.report_best_type == '0'
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80 --best 0
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81 #else if $report.report_best.report_best_type == '1'
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82 --best 1
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83 --min_lis $report.report_best.report_best_min_lis
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84 #else
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85 --best $report.report_best.report_best_value
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86 --min_lis $report.report_best.report_best_min_lis
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87 #end if
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88 #else
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89 #if $report.report_num_alignments.report_num_alignments_type == 'other_value'
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90 --num_alignments $report.report_num_alignments.report_num_alignments_value
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91 #else
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92 --num_alignments $report.report_num_alignments.report_num_alignments_type
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93 #end if
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94 #end if
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95
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96 -e $e_value
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97 --match $match
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98 --mismatch $mismatch
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99 --gap_open $gap_open
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100 --gap_ext $gap_ext
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101 -N $ambiguous_letter
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102 -a \${GALAXY_SLOTS:-1}
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103 ]]>
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104 </command>
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105 <inputs>
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106 <param format="fasta,fastq" name="input_reads" type="data" label="Querying sequences" help="In FASTA or FASTQ format (--reads)"/>
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107 <conditional name="sequencing_type">
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108 <param name="sequencing_type_selector" type="select" label="Sequencing type">
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109 <option value="not_paired">Reads are not paired</option>
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110 <option value="paired">Reads are paired</option>
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111 </param>
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112 <when value="not_paired" />
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113 <when value="paired">
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114 <param name="paired_type" type="select" display="radio" label="If one of the paired-end reads aligns and the other one does not">
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115 <option value="">leave the reads split between aligned and rejected files</option>
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116 <option value="--paired-in">output both reads to aligned file (--paired-in)</option>
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117 <option value="--paired-out">output both reads to rejected file (--paired-out)</option>
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118 </param>
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119 </when>
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120 </conditional>
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121
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122 <param name="strand_search" type="select" label="Which strands to search">
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123 <option value="">Search both strands</option>
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124 <option value="-F">Search only the forward strand (-F)</option>
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125 <option value="-R">Search only the reverse-complementary strand (-R)</option>
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126 </param>
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127
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128 <conditional name="databases_type">
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129 <param name="databases_selector" type="select" label="Databases to query"
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130 help="Public rRNA databases provided with SortMeRNA have been indexed.
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131 On the contrary, personal databases must be indexed each time SortMeRNA is launched.
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132 Please be patient, this may take some time depending on the size of the given database.">
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133 <option value="cached" selected="true">Public pre-indexed ribosomal databases</option>
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134 <option value="cached_to_index">Public ribosomal databases to index with non default parameters</option>
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135 <option value="history">Databases from your history</option>
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136 </param>
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137 <when value="cached">
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138 <param name="input_databases" label="rRNA databases" type="select" display="checkboxes" multiple="true">
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139 <options from_data_table="rRNA_databases" />
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140 <validator type="no_options" message="Select at least one database"/>
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141 </param>
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142 </when>
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143 <when value="cached_to_index">
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144 <param name="input_databases" label="rRNA databases" type="select" display="checkboxes" multiple="true">
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145 <options from_data_table="rRNA_databases" />
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146 <validator type="no_options" message="Select at least one database"/>
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147 </param>
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148 <param name="seed_length" type="integer" min="0" max="100" value="18" label="Seed length for database indexing" help="(-L)"/>
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149 <param name="max_pos" type="integer" min="0" max="100000" value="10000" label="Maximum number of positions to store for each k-mer for database indexing" help="With 0, all positions are stored (--max_pos)"/>
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150 </when>
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151 <when value="history">
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152 <param name="database_name" type="data" format="fasta" multiple="true" label="rRNA databases"
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153 help="Your databases will be indexed first, which may take up to several minutes."/>
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154 <param name="seed_length" type="integer" min="0" max="100" value="18" label="Seed length for database indexing" help="(-L)"/>
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155 <param name="max_pos" type="integer" min="0" max="100000" value="10000" label="Maximum number of positions to store for each k-mer for database indexing" help="With 0, all positions are stored (--max_pos)"/>
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156 </when>
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157 </conditional>
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158
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159 <!-- Outputs -->
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160 <conditional name="aligned_fastx">
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161 <param name="aligned_fastx_selector" type="select" label="Include aligned reads in FASTA/FASTQ format?">
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162 <option value="--fastx">Yes (--fastx)</option>
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163 <option value="">No</option>
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164 </param>
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165 <when value="--fastx">
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166 <param name="other" type="boolean" label="Include rejected reads file?" help="(--other)" />
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167 </when>
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168 <when value="" />
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169 </conditional>
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170 <conditional name="aligned_sam">
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171 <param name="aligned_sam_selector" type="select" label="Include alignments in SAM format?">
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172 <option value="--sam">Yes (--sam)</option>
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173 <option value="">No</option>
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174 </param>
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175 <when value="--sam">
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176 <param name="sq" type="boolean" truevalue="--SQ" falsevalue="" label="Add SQ tags to the SAM file" help="(--SQ)" />
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177 </when>
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178 <when value="" />
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179 </conditional>
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180 <param name="aligned_blast" type="select" label="Include alignments in BLAST-like format">
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181 <option value="--blast 0">pairwise (--blast 0)</option>
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182 <option value="--blast 1">tabular BLAST -m 8 format (--blast 1)</option>
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183 <option value="--blast 2">tabular + column for CIGAR (--blast 2)</option>
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184 <option value="--blast 3">tabular + columns for CIGAR and query coverage (--blast 3)</option>
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185 <option value="" selected="true">No</option>
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186 </param>
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187 <param name="log" type="boolean" checked="False" truevalue="--log" falsevalue="" label="Generate statistics file"
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188 help="Generates statistics for the rRNA content of reads, as well as rRNA subunit distribution. (--log)">
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189 </param>
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190 <conditional name="report">
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191 <param name="report_type" type="select" label="Parameters for filtering and read mapping" help="">
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192 <option value="best" selected="true">Report best alignments per read reaching E-value</option>
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193 <option value="num_alignments">Report first alignements per read reaching E-value</option>
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194 </param>
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195 <when value="best">
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196 <conditional name="report_best">
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197 <param name="report_best_type" type="select" label="Number of searched alignments" help="Only the best alignment is reported (--best)">
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198 <option value="0">All high-candidate reference sequences are searched for alignments (very slow)</option>
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199 <option value="1" selected="true">Only one high-candidate reference sequence is searched for alignments (fast). The high-candidate sequences are determined heuristically using a LIS of seed matches)</option>
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200 <option value="other_value">A custom number of reference sequences are searched for alignments (speed decrease for high value)</option>
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201 </param>
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202 <when value="0" />
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203 <when value="1">
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204 <param name="report_best_min_lis" type="integer" min="0" max="100" value="2" label="Number of longest LIS an alignement needs to be searched" help="The alignements having the first INT longest LIS. LIS stands for Longest Increasing Subsequence, it is computed using seeds' positions to expand hits into longer matches prior to Smith-Waterman alignment. (--min_lis)"/>
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205 </when>
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206 <when value="other_value">
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207 <param name="report_best_value" type="integer" min="2" max="100" value="2" label="Number of alignments to be made" help="Only the best one is reported. The computation speed decrease with high value"/>
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208 <param name="report_best_min_lis" type="integer" min="0" max="100" value="2" label="Number of longest LIS an alignement needs to be searched" help="The alignements having the first INT longest LIS. LIS stands for Longest Increasing Subsequence, it is computed using seeds' positions to expand hits into longer matches prior to Smith-Waterman alignment. (--min_lis)"/>
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209 </when>
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210 </conditional>
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211 </when>
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212 <when value="num_alignments">
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213 <conditional name="report_num_alignments">
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214 <param name="report_num_alignments_type" type="select" label="Number of output alignments" help="(--num_alignments)">
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215 <option value="0">All alignments reaching the E-value threshold are reported (very slow, this option is not suggested for high similarity rRNA databases)</option>
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216 <option value="1" selected="true">The first alignment passing E-value threshold are reported (very fast, best choice if only filtering is needed)</option>
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217 <option value="other_value">A custom number of alignments are made and reported (speed decrease for high value)</option>
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218 </param>
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219 <when value="0" />
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220 <when value="1" />
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221 <when value="other_value">
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222 <param name="report_num_alignments_value" type="integer" min="0" max="100" value="1" label="Number of alignments to be made and reported" help=""/>
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223 </when>
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224 </conditional>
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225 </when>
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226 </conditional>
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227
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228 <param name="e_value" type="float" min="0" max="10" value="1" label="E-value threshold" help="(-e)"/>
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229 <param name="match" type="integer" min="0" max="10" value="2" label="SW score for a match" help="(--match)"/>
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230 <param name="mismatch" type="integer" min="-10" max="0" value="-3" label="SW penalty for a mismatch" help="(--mismatch)"/>
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231 <param name="gap_open" type="integer" min="0" max="10" value="5" label="SW penalty for introducing a gap" help="(--gap_open)"/>
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232 <param name="gap_ext" type="integer" min="0" max="10" value="2" label="SW penalty for extending a gap" help="(--gap_ext)"/>
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233 <param name="ambiguous_letter" type="integer" min="-10" max="0" value="-3" label="SW penalty for ambiguous letters (N's)" help="(-N)"/>
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234 </inputs>
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235 <outputs>
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236 <data format_source="input_reads" name="output_fastx" from_work_dir="aligned.dat"
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237 label="Aligned reads on ${on_string} (${input_reads.datatype.file_ext})">
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238 <filter>aligned_fastx['aligned_fastx_selector']</filter>
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239 </data>
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240 <data format_source="input_reads" name="output_other" from_work_dir="other_file.dat"
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241 label="Rejected reads on ${on_string} (${input_reads.datatype.file_ext})">
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242 <filter>aligned_fastx['aligned_fastx_selector'] and aligned_fastx['other']</filter>
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243 </data>
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244 <data format="sam" name="output_sam" from_work_dir="aligned.sam"
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245 label="Alignments on ${on_string} (SAM)">
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246 <filter>aligned_sam['aligned_sam_selector']</filter>
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247 </data>
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248 <data format="tabular" name="output_blast" from_work_dir="aligned.blast"
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249 label="Alignments on ${on_string} (BLAST)">
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250 <filter>aligned_blast</filter>
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251 <change_format>
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252 <when input="aligned_blast" value="--blast 0" format="txt" />
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253 </change_format>
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254 </data>
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255 <data format="txt" name="output_log" label="${tool.name} statistics (txt)" from_work_dir="aligned.log">
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256 <filter>log</filter>
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257 </data>
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258 </outputs>
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259 <tests>
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260 <test>
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261 <param name="input_reads" value="read_small.fastq" />
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262 <param name="sequencing_type_selector" value="not_paired" />
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263 <param name="strand_search" value="" />
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264 <param name="databases_selector" value="history" />
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265 <param name="database_name" value="ref_small.fasta" />
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266 <param name="other" value="True" />
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267 <param name="log" value="" />
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268 <output name="output_fastx" file="sortmerna_wrapper_accept1.fastq" />
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269 <output name="output_other" file="sortmerna_wrapper_other1.fastq" />
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270 <output name="output_sam" file="sortmerna_wrapper_sam1.sam" lines_diff="2" />
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271 </test>
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272 <test>
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273 <param name="input_reads" value="read_small.fasta" />
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274 <param name="sequencing_type_selector" value="not_paired" />
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275 <param name="strand_search" value="" />
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276 <param name="databases_selector" value="history" />
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277 <param name="database_name" value="ref_small.fasta" />
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278 <param name="other" value="True" />
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279 <param name="log" value="" />
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280 <output name="output_fastx" file="sortmerna_wrapper_accept2.fasta" />
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281 <output name="output_other" file="sortmerna_wrapper_other2.fasta" />
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282 <output name="output_sam" file="sortmerna_wrapper_sam2.sam" lines_diff="2" />
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283 </test>
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284 </tests>
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285 <help>
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286 <![CDATA[
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287 **What it does**
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288
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289 SortMeRNA_ is a software designed to rapidly filter ribosomal RNA fragments
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290 from metatransriptomic data produced by next-generation sequencers.
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291 It is capable of handling large RNA databases and sorting out all fragments
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292 matching to the database with high accuracy and specificity.
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293
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294 .. _SortMeRNA: http://bioinfo.lifl.fr/RNA/sortmerna/
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295
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296
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297 **Input**
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298
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299 The input is one file of reads in FASTA or FASTQ format and any number of rRNA databases to search against.
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300 If the user has two foward-reverse paired-sequencing reads files, they may use
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301 the script "merge_paired_reads.sh" to interleave the reads into one file, preserving their order.
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302
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303 If the sequencing type for the reads is paired-ended, the user has two options under
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304 "Sequencing type" to filter the reads and preserve their order in the file.
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305 For a further example of each option, please refer to Section 4.2.3 in the `SortMeRNA User Manual`_.
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306
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307 .. _sortmerna user manual: http://bioinfo.lifl.fr/RNA/sortmerna/code/SortMeRNA-user-manual-v1.7.pdf
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308
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309
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310 **Output**
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311
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312 The output will follow the same format (FASTA or FASTQ) as the reads. Optionally, a statistic file for the rRNA content of reads, as well as rRNA subunit distribution can be generated.
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313
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314
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315 **rRNA databases**
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316
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317 SortMeRNA is distributed with 8 representative rRNA databases, which were
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318 all constructed from the SILVA SSU,LSU (version 111) and the RFAM 5/5.8S
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319 (version 11.0) databases using the tool UCLUST.
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320
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321 +--------------------------+------+-------------+-------------------+------------------------+-------------------+
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322 | Representative database | id % | average id% | # seq (clustered) | Origin | # seq (original) |
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323 +==========================+======+=============+===================+========================+===================+
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324 | SILVA 16S bacteria | 85 | 91.6 | 8174 | SILVA SSU Ref NR v.111 | 244077 |
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325 +--------------------------+------+-------------+-------------------+------------------------+-------------------+
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326 | SILVA 16S archaea | 95 | 96.7 | 3845 | SILVA SSU Ref NR v.111 | 10919 |
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327 +--------------------------+------+-------------+-------------------+------------------------+-------------------+
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328 | SILVA 18S eukarya | 95 | 96.7 | 4512 | SILVA SSU Ref NR v.111 | 31862 |
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329 +--------------------------+------+-------------+-------------------+------------------------+-------------------+
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330 | SILVA 23S bacteria | 98 | 99.4 | 3055 | SILVA LSU Ref v.111 | 19580 |
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331 +--------------------------+------+-------------+-------------------+------------------------+-------------------+
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332 | SILVA 23s archaea | 98 | 99.5 | 164 | SILVA LSU Ref v.111 | 405 |
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333 +--------------------------+------+-------------+-------------------+------------------------+-------------------+
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334 | SILVA 28S eukarya | 98 | 99.1 | 4578 | SILVA LSU Ref v.111 | 9321 |
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335 +--------------------------+------+-------------+-------------------+------------------------+-------------------+
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336 | Rfam 5S archaea/bacteria | 98 | 99.2 | 59513 | RFAM | 116760 |
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337 +--------------------------+------+-------------+-------------------+------------------------+-------------------+
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338 | Rfam 5.8S eukarya | 98 | 98.9 | 13034 | RFAM | 225185 |
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339 +--------------------------+------+-------------+-------------------+------------------------+-------------------+
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340
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341 id %: members of the cluster must have identity at least 'id %' identity with the representative sequence
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342
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343 average id %: average identity of a cluster member to the representative sequence
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344
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345 The user may also choose to use their own rRNA databases.
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346
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347 .. class:: warningmark
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348
5
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349 Note that your personal databases are indexed each time. The public ribosomal
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350 databases are indexed when added, but they can be re-indexed with non-default indexing
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351 parameters. The indexing may take some time depending on the size of the given database.
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352
3
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353 ]]>
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354 </help>
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355
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356 <citations>
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357 <citation type="doi">10.1093/bioinformatics/bts611</citation>
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358 <citation type="doi">10.1093/nar/gks1219</citation>
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359 <citation type="doi">10.1093/nar/gks1005</citation>
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360 <citation type="doi">10.1093/bioinformatics/btq461</citation>
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361 <citation type="doi">10.1038/nbt.2198</citation>
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362 </citations>
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363 </tool>