Mercurial > repos > pjbriggs > macs21
comparison README.rst @ 9:7aecd0908b3c draft
Uploaded version 2.1.0-4.
author | pjbriggs |
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date | Mon, 15 Jun 2015 06:06:48 -0400 |
parents | a881c5b7162c |
children | f346287fe52c |
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1 MACS21: Model-based Analysis of ChIP-Seq (MACS 2.1.0) | 1 MACS21: Model-based Analysis of ChIP-Seq (MACS 2.1.0) peak calling |
2 ===================================================== | 2 ================================================================== |
3 | 3 |
4 Galaxy tool wrapper for the MACS 2.1.0 ChIP-seq peak calling program. MACS has been | 4 Galaxy tool wrapper for the peak calling function of MACS 2.1.0. MACS has been |
5 developed by Tao Lui | 5 developed by Tao Lui |
6 https://github.com/taoliu/MACS/ | 6 https://github.com/taoliu/MACS/ |
7 | 7 |
8 The reference for MACS is: | 8 The reference for MACS is: |
9 | 9 |
29 need to modify the ``tools_conf.xml`` file to tell Galaxy to offer the tool | 29 need to modify the ``tools_conf.xml`` file to tell Galaxy to offer the tool |
30 by adding the line: | 30 by adding the line: |
31 | 31 |
32 <tool file="macs21/macs21_wrapper.xml" /> | 32 <tool file="macs21/macs21_wrapper.xml" /> |
33 | 33 |
34 You will also need to install MACS 2.1.0: | 34 You will also need to install MACS 2.1.0 and its dependencies: |
35 | 35 |
36 - https://pypi.python.org/pypi/MACS2 | 36 - https://pypi.python.org/pypi/MACS2 |
37 | 37 |
38 and ensure that it's on your Galaxy user's ``PATH`` when running the tool. | 38 and ensure that it's on your Galaxy user's ``PATH`` when running the tool. |
39 | 39 |
40 If you want to run the functional tests, copy the sample test files under | 40 If you want to run the functional tests, copy the sample test files under |
41 sample test files under Galaxy's ``test-data/`` directory. Then: | 41 sample test files under Galaxy's ``test-data/`` directory. Then: |
42 | 42 |
43 ./run_tests.sh -id macs2_wrapper | 43 ./run_tests.sh -id macs2_wrapper |
44 | 44 |
45 (However there are no tests defined at present.) | |
46 | 45 |
47 History | 46 History |
48 ======= | 47 ======= |
49 | 48 |
50 This tool is based on the ``modencode-dcc`` MACS2 tool developed by Ziru Zhou | 49 This tool was originally based on the ``modencode-dcc`` MACS2 tool developed |
51 (ziruzhou@gmail.com), specifically the ``16:14f378e35191`` revision of the | 50 by Ziru Zhou (ziruzhou@gmail.com), specifically the ``16:14f378e35191`` |
52 tool at | 51 revision of the tool available via |
53 | 52 |
54 - http://toolshed.g2.bx.psu.edu/view/modencode-dcc/macs2 | 53 - http://toolshed.g2.bx.psu.edu/view/modencode-dcc/macs2 |
55 | 54 |
56 It has been substantially modified both to adapt it to MACS 2.1.0, and to | 55 This version has been substantially modified both to adapt it to MACS 2.1.0, and |
57 re-implement the internal workings of the tool to conform with current practices | 56 to re-implement the internal workings of the tool to conform with current |
58 in invoking commands from Galaxy. | 57 practices in invoking commands from Galaxy, and to add new functionality. |
59 | 58 |
60 ========== ====================================================================== | 59 ========== ====================================================================== |
61 Version Changes | 60 Version Changes |
62 ---------- ---------------------------------------------------------------------- | 61 ---------- ---------------------------------------------------------------------- |
62 2.1.0-4 - Remove 'bdgcmp' functionality. | |
63 2.1.0-3 - Add tool tests | |
63 2.1.0-2 - Add option to create bigWig file from bedGraphs; fix bug with -B | 64 2.1.0-2 - Add option to create bigWig file from bedGraphs; fix bug with -B |
64 option; make --mfold defaults consistent. | 65 option; make --mfold defaults consistent. |
65 2.1.0-1 - Initial version | 66 2.1.0-1 - Initial version |
66 ========== ====================================================================== | 67 ========== ====================================================================== |
67 | 68 |