Mercurial > repos > mvdbeek > mismatch_frequencies
changeset 16:a86213925f14
Uploaded
author | mvdbeek |
---|---|
date | Wed, 01 Apr 2015 08:26:59 -0400 |
parents | 7c5b252bf6af |
children | deac50041967 |
files | mismatch_frequencies.xml tool_dependencies.xml |
diffstat | 2 files changed, 6 insertions(+), 34 deletions(-) [+] |
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--- a/mismatch_frequencies.xml Wed Apr 01 06:04:37 2015 -0400 +++ b/mismatch_frequencies.xml Wed Apr 01 08:26:59 2015 -0400 @@ -3,7 +3,7 @@ <requirements> <requirement type="package" version="0.7.7">pysam</requirement> <requirement type="package" version="0.14.1">pandas</requirement> - <requirement type="package" version="1.4">matplotlib</requirement> + <requirement type="package" version="1.2">matplotlib</requirement> </requirements> <command interpreter="python">mismatch_frequencies.py --input #for i in $rep @@ -19,8 +19,8 @@ --three_p $three_p </command> <inputs> - <repeat name="rep" title="alignment files" min="1"> - <param name="input_file" type="data" format="bam,sam" label="Alignment file" help="The input alignment file(s) for which you want to calculate mismatch frequencies."/> + <repeat name="rep" title="alignment files"> + <param name="input_file" type="data" format="bam,sam" label="Alignment file" help="The input alignment file(s) for which to analyze the mismatches."/> </repeat> <param name="number_of_mismatches" label="Maximum number of allowed mismatches per read" help="Discard reads with more than the chosen number of mismatches from the frequency calculation" type="integer" value="3"/> <param name="min_length" label="Minumum read length to analyse" type="integer" value="21"/> @@ -35,36 +35,8 @@ <tests> <test> <param name="rep_0|input_file" value="3mismatches_ago2ip_s2.bam" ftype="bam" /> - <param name="rep_1|input_file" value="3mismatches_ago2ip_ovary.bam" ftype="bam" /> + <param name="rep_1|input_file" value="3mismatches_ago2ip_s2.bam" ftype="bam" /> <param name="number_of_mismatches" value="1" /> <param name="min_length" value="21" /> <param name="max_length" value="21" /> - <output name="tabular" file="mismatch.tab" ftype="tabular"/> - </test> - </tests> - <help> - -.. class:: infomark - - -***What it does*** - -This tool reconstitues for each aligned read of an alignment file in SAM/BAM format whether -a mismatch is annotated in the MD tag, and if that is the case counts the identity of the -mismatch relative to the reference sequence. The output is a PDF document with the calculated -frequency for each mismatch that occured relative to the total number of valid reads and a table -with the corresponding values. Read length can be limited to a specific read length, and 5 prime and -3 prime-most nucleotides of a read can be ignored. - ----- - -.. class:: warningmark - -***Warning*** - -This tool skips all read that have insertions and has been tested only with bowtie and bowtie2 -generated alignment files. - -Written by Marius van den Beek, m.vandenbeek at gmail . com - </help> </tool>
--- a/tool_dependencies.xml Wed Apr 01 06:04:37 2015 -0400 +++ b/tool_dependencies.xml Wed Apr 01 08:26:59 2015 -0400 @@ -6,7 +6,7 @@ <package name="pandas" version="0.14.1"> <repository changeset_revision="ef98e20431a7" name="package_pandas_0_14" owner="iuc" toolshed="https://testtoolshed.g2.bx.psu.edu" /> </package> - <package name="matplotlib" version="1.4"> - <repository changeset_revision="62a48352f6a6" name="package_matplotlib_1_4" owner="iuc" toolshed="https://testtoolshed.g2.bx.psu.edu" /> + <package name="matplotlib" version="1.2"> + <repository changeset_revision="9f3e58477115" name="package_matplotlib_1_2" owner="iuc" toolshed="https://testtoolshed.g2.bx.psu.edu" /> </package> </tool_dependency>