Mercurial > repos > mvdbeek > igv_take_screenshots

annotate igv_make_screenshots.xml @ 20:1f8609ae3941 draft

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planemo upload for repository https://github.com/bardin-lab/readtagger/tree/master/galaxy commit be45e3b0dec4ca1e8611abb2d84dd995e6d53751-dirty
author mvdbeek
date Mon, 31 Jul 2017 12:45:19 -0400
parents fd2d19ae2648
children de2f2c28c498
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mvdbeek
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1 <tool id="igv_make_screenshots" name="IGV_screenshots" version="0.1.1">
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2 <requirements>
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3 <requirement type="package" version="0.2.9">xvfbwrapper</requirement>
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4 <requirement type="package" version="2.3.93">igv</requirement>
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5 </requirements>
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6 <command detect_errors="exit_code"><![CDATA[
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7 #import re
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8 #if $genome_source.input_type_selector == 'history':
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9 ln -s '$genome_source.genome' genome.fa &&
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10 #else:
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11 ln -s '$genome_source.genome.fields.path' genome.fa &&
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12 #end if
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13 samtools faidx genome.fa &&
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14 sed -i.bak -e "s|\"genome.fa\"|\"\$PWD/genome.fa\"|g" '$igv_session' &&
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15 #for $i, $inputsection in enumerate($inputfiles):
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16 #set $path="%s.%s.%s" % ($i, re.sub('[^\w\-_]', '_', $inputsection.input.element_identifier), $inputsection.input.extension)
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17 sed -i.bak -e "s|\"$path\"|\"\$PWD/$path\"|g" '$igv_session' &&
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18 ln -fs '$inputsection.input' '$path' &&
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19 #if $inputsection.input.is_of_type('bam')
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20 ln -fs $inputsection.input.metadata.bam_index '$path'.bai &&
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21 #end if
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22 #end for
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23 ln -fs $igv_session igv_session.xml &&
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24 cat '$load_session' > load_session.txt &&
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25 echo snapshotDirectory "\$PWD" >> load_session.txt &&
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26 cat load_session.txt '$script_file' '$exit_session' > igv_script.txt &&
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27 cp '$igv_session' '$igv_session_out' &&
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28 python $__tool_directory__/xvfb_igv.py igv_script.txt '$igv_preferences' $width,$height &&
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29 zip screenshots.zip *.png &&
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30 cp screenshots.zip '$screenshots_out'
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31 ]]></command>
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32 <configfiles>
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33 <configfile name="igv_session">#import re
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34 <![CDATA[<?xml version="1.0" encoding="UTF-8" standalone="no"?>
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35 <Session genome="genome.fa" hasGeneTrack="false" hasSequenceTrack="true" version="8">
0
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36 <Resources>
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37 #for $i, $inputsection in enumerate($inputfiles):
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38 #set $path="%s.%s.%s" % ($i, re.sub('[^\w\-_]', '_', $inputsection.input.element_identifier), $inputsection.input.extension)
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39 #set $coverage_id="%s_coverage" % $path
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40 <Resource path="$path"/>
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41 #end for
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42 </Resources>
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43 #for $i, $inputsection in enumerate($inputfiles):
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44 #if $inputsection.input.is_of_type('bam')
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45 #set $label=str($inputsection.label) if str($inputsection.label) else str($inputsection.input.element_identifier)
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46 #set $path="%s.%s.%s" % ($i, re.sub('[^\w\-_]', '_', $inputsection.input.element_identifier), $inputsection.input.extension)
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47 <Panel height="$inputsection.section_height" name="Panel${label}" width="$width">
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48 ## First track is the coverage
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49 <Track altColor="0,0,178" autoScale="true" color="175,175,175" colorScale="ContinuousColorScale;0.0;10.0;255,255,255;175,175,175" displayMode="COLLAPSED" featureVisibilityWindow="-1" fontSize="10" id="$coverage_id" name="$label Coverage" showReference="false" snpThreshold="0.2" sortable="true" visible="true">
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50 <DataRange baseline="0.0" drawBaseline="true" flipAxis="false" maximum="10.0" minimum="0.0" type="LINEAR"/>
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51 </Track>
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52 ## Second track is the actual BAM alignment file
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53 <Track altColor="0,0,178" autoScale="false" color="0,0,178" displayMode="EXPANDED" featureVisibilityWindow="-1" fontSize="10" id="$path" name="$label" sortable="true" visible="true">
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54 <RenderOptions colorByTag="$inputsection.color_by_tag" colorOption="TAG" flagUnmappedPairs="false" groupByOption="NONE" groupByTag="BR" linkByTag="READNAME" linkedReads="false" maxInsertSize="1000" minInsertSize="50" quickConsensusMode="false" shadeBasesOption="QUALITY" shadeCenters="true" showAllBases="false" sortByTag="" viewPairs="false"/>
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55 </Track>
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56 </Panel>
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57 #end if
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58 #end for
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59 <Panel height="186" name="FeaturePanel" width="$width">
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60 <Track altColor="0,0,178" autoScale="false" color="0,0,178" displayMode="COLLAPSED" featureVisibilityWindow="-1" fontSize="10" id="Reference sequence" name="Reference sequence" sortable="false" visible="true"/>
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61 #for $i, $inputsection in enumerate($inputfiles):
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62 #if not $inputsection.input.is_of_type('bam')
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63 #set $label=str($inputsection.label) if str($inputsection.label) else str($inputsection.input.element_identifier)
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64 #set $path="%s.%s.%s" % ($i, re.sub('[^\w\-_]', '_', $inputsection.input.element_identifier), $inputsection.input.extension)
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65 <Track altColor="0,0,178" autoScale="false" clazz="org.broad.igv.track.FeatureTrack" color="0,0,178" displayMode="COLLAPSED" featureVisibilityWindow="-1" fontSize="10" id="$path" name="$label" renderer="BASIC_FEATURE" sortable="false" visible="true" windowFunction="count"/>
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66 #end if
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67 #end for
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68 </Panel>
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69 <PanelLayout dividerFractions="0.004995836802664446,0.12905911740216486,0.2681099084096586,0.5512073272273106,0.8409658617818485"/>
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70 </Session>
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71 ]]></configfile>
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72 <configfile name="load_session">
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73 load igv_session.xml
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74 </configfile>
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75 <configfile name="exit_session">
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76 exit
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77 </configfile>
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78 <configfile name="igv_preferences">
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79 SAM.SHOW_SOFT_CLIPPED=$show_softclippped
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80 SAM.FILTER_SECONDARY_ALIGNMENTS=$filter_secondary
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81 </configfile>
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82 </configfiles>
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83 <inputs>
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84 <conditional name="genome_source">
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85 <param name="input_type_selector" type="select" label="Choose the genome source">
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86 <option value="cached" selected="True">Built-in references</option>
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87 <option value="history">Select a fasta file from your history</option>
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88 </param>
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89 <when value="cached">
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90 <param name="genome" type="select" label="Select a genome">
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91 <options from_data_table="all_fasta" />
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92 </param>
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93 </when>
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94 <when value="history">
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95 <param name="genome" type="data" format="fasta" label="Select a fasta file as reference genome"/>
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96 </when>
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97 </conditional>
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98 <repeat name="inputfiles" min="1" title="Add tracks">
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99 <param name="input" type="data" format="bam,gff,gff3,gtf,bed,vcf" label="Choose an input file"/>
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100 <param name="label" type="text" label="Enter a label for this file. If no label is entered the history name will be used"/>
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101 <param name="section_height" type="integer" value="300" label="Height for this track"/>
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102 <param name="color_by_tag" type="text" value="CD" label="Enter a BAM/SAM tag that should detrmine the color of a read">
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103 <sanitizer invalid_char="">
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104 <valid initial="string.letters,string.digits"><add value="_" /> </valid>
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105 </sanitizer>
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106 </param>
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107 </repeat>
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108 <param name="show_softclippped" type="boolean" label="Show softclipped bases?" truevalue="true" falsevalue="false" checked="true"/>
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109 <param name="filter_secondary" type="boolean" label="Filter out secondary reads" truevalue="true" falsevalue="false" checked="true"/>
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110 <param name="script_file" type="data" format="txt" label="Select a IGV script with regions for which to take screenshots"/>
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111 <param name="width" label="Select the screenshot width" type="integer" min="800" value="1920"/>
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112 <param name="height" label="Select the screenshot height" type="integer" min="640" value="1080"/>
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113 </inputs>
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114 <outputs>
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115 <data name="igv_session_out" format="xml"/>
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116 <data name="screenshots_out" format="zip"/>
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117 </outputs>
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118 <tests>
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119 <test>
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120 <param name="genome_source|input_type_selector" value="history"/>
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121 <param name="genome_source|genome" value="rover_reference.fa"/>
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122 <repeat name="inputfiles">
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123 <param name="input" value="rover_corrected.bam"/>
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124 </repeat>
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125 <output name="igv_session_out" file="igv_session.xml" lines_diff="6"/>
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126 </test>
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127 </tests>
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128 <help><![CDATA[
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129 This tool can automate taking screenshots using IGV.
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130 It takes as input a reference genome, an IGV script file (bedtools igv can produce such a file)
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131 and a variable number of BAM,GFF,GTF,BED or VCF files, and produces a zip file containing screenshots for each genomic region in the IGV script file.
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132 ]]></help>
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133 <citations>
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134 <citation type="doi">10.1093/bib/bbs017</citation>
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135 </citations>
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136 </tool>