Mercurial > repos > iuc > sra_tools

annotate sam_dump.xml @ 15:54366e4d692a draft

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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sra-tools commit 2be63abd7e4db27fa32ecbbc10d48cacb0073115"
author iuc
date Fri, 20 Mar 2020 16:36:52 +0000
parents 5c5d6624f8de
children 37f09413ddbf
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15
54366e4d692a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sra-tools commit 2be63abd7e4db27fa32ecbbc10d48cacb0073115"
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1 <tool id="sam_dump" name="Download and Extract Reads in BAM" version="@VERSION@+galaxy1" profile="18.01">
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2 <description>format from NCBI SRA</description>
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3 <macros>
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4 <import>sra_macros.xml</import>
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5 </macros>
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6 <expand macro="requirements">
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7 <requirement type="package" version="1.10">samtools</requirement>
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8 </expand>
0
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9 <version_command>sam-dump --version</version_command>
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10 <command detect_errors="exit_code">
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11 <![CDATA[
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12 @SET_ACCESSIONS@
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13
0
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14 ## Need to set the home directory to the current working directory,
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15 ## else the tool tries to write to home/.ncbi and fails when used
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16 ## with a cluster manager.
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17 @CONFIGURE_TIMEOUT@
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18 vdb-config -s "/repository/user/main/public/root=\$PWD" &&
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19 ## Do not use prefetch if region is specified, to avoid downloading
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20 ## the complete sra file.
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22 #if $input.input_select == "file":
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23 sam-dump --log-level fatal '${input.file.name}'
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24 #else:
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25 #if ( str( $adv.region ) == "" ):
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26 prefetch -X 200000000 "\$acc" &&
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27 ## Duplicate vdb-config, in case settings changed between prefetch and
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28 ## dump command.
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29 vdb-config -s "/repository/user/main/public/root=\$PWD" &&
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30 #end if
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31 sam-dump --log-level fatal --disable-multithreading
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32 #end if
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33
0
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34 #if str( $adv.region ) != "":
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35 --aligned-region '$adv.region'
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36 #end if
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37 #if str( $adv.matepairDist ) != "":
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38 --matepair-distance '$adv.matepairDist'
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39 #end if
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40 #if str( $adv.minMapq ) != "":
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41 --min-mapq '$adv.minMapq'
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42 #end if
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43 --header
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44 #if str( $adv.alignments ) == "both":
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45 --unaligned
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46 #end if
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47
0
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48 #if str( $adv.alignments ) == "unaligned":
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49 --unaligned-spots-only
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50 #end if
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51 #if (str( $adv.primary ) == "yes") and (str ( $adv.alignments != "unaligned") ):
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52 --primary
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53 #end if
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54 #if $input.input_select == "file":
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55 '$input.file'
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56 #elif $input.input_select == "accession_number":
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57 "\$acc"
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58 #elif $input.input_select=="file_list":
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59 "\$acc"
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60 #end if
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61
0
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62 #if str( $outputformat ) == "bam":
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63 | samtools view -Sb - 2> /dev/null
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64 #end if
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65 #if $input.input_select == "file":
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66 > '$output_file'
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67 #elif $input.input_select == "accession_number":
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68 > '$output_accession' )
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69 #end if
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70
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71 #if $input.input_select=="file_list":
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72 #if str( $outputformat ) == "bam":
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73 > "\$acc.bam"
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74 #elif str( $outputformat ) == "sam":
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75 > "\$acc.sam"
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76 #end if
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77 ) ; done
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78 #end if
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79
0
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80 ]]>
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81 </command>
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82 <inputs>
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83 <expand macro="input_conditional"/>
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84 <param name="outputformat" type="select" display="radio" label="select output format" help="In vast majority of cases you want to download data in bam format. It is more compact and is accepted by all downstream tools.">
0
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85 <option value="bam">bam</option>
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86 <option value="sam">sam</option>
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87 </param>
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88 <section name="adv" title="Advanced Options" expanded="False">
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89 <expand macro="alignments"/>
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90 <expand macro="region"/>
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91 <expand macro="matepairDist"/>
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92 <param name="primary" type="select" value="no">
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93 <label>only primary aligments</label>
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94 <option value="no">No</option>
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95 <option value="yes">Yes</option>
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96 </param>
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97 <expand macro="minMapq"></expand>
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98 </section>
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99 </inputs>
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100 <outputs>
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101 <collection name="output_collection" type="list" label="SAM/BAM data (fastq-dump)">
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102 <filter>input['input_select'] == "file_list"</filter>
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103 <discover_datasets pattern="(?P&lt;designation&gt;.+)\.bam" directory="." ext='bam'/>
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104 <discover_datasets pattern="(?P&lt;designation&gt;.+)\.sam" directory="." ext='sam'/>
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105 </collection>
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106 <data name="output_accession" format="bam" label="${input.accession} (sam-dump)">
0
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107 <filter>input['input_select'] == "accession_number"</filter>
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108 <change_format>
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109 <when input="outputformat" value="sam" format="sam"/>
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110 </change_format>
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111 </data>
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112 <data name="output_file" format="bam" label="${input.file.name} (sam-dump)">
0
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113 <filter>input['input_select'] == "file"</filter>
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114 <change_format>
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115 <when input="outputformat" value="sam" format="sam"/>
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116 </change_format>
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117 </data>
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118 </outputs>
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119 <tests>
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120 <test>
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121 <param name="input_select" value="accession_number"/>
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122 <param name="accession" value="SRR925743"/>
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123 <param name="outputformat" value="sam"/>
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124 <param name="region" value="17:41243452-41277500"/>
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125 <output name="output_accession" file="sam_dump_result.sam" compare="contains" ftype="sam"/>
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126 </test>
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127 </tests>
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128 <help><![CDATA[
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129
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130 **What it does?**
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131
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132 This tool extracts data (in BAM_ format) from the Short Read Archive (SRA) at the National Center for Biotechnology Information (NCBI). It is based on the sam-dump_ utility of the SRA Toolkit.
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133
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134 **How to use it?**
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135
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136 There are three ways in which you can download data:
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137
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138 1. Data for single accession
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139 2. Multiple datasets using a list of accessions
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140 3. Extract data from already uploaded SRA dataset
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141
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142 Below we discuss each in detail.
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143
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144 ------
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145
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146 **Uploading data for a single accession**
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147
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148 When you type a single accession number (e.g., `SRR1582967`) into **Accession** box and click **Execute** the tool will fetch data for you. As a result you will get a single BAM (or SAM) dataset in the history.
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149
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150 -----
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151
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152 **Uploading multiple datasets using a list of accessions**
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153
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154 A more realistic scenario is when you want to upload a number of datasets at once. To do this you need a list of accession, where there is only one accession per line (see below for information on how to generate such a file). Once you have this file:
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155
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156 1. Upload it into your history using Galaxy's upload tool
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157 2. Once the list of accessions is uploaded choose *List of SRA accessions, one per line* from **select input type** dropdown
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158 3. Choose uploaded file within the **sra accession list** field
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159 4. Click **Execute**
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160
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161 .. class:: warningmark
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162
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163 BAM datasets produced by this option will be saved in Galaxy's history as a collection_ - a single history element containing multiple datasets.
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164
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165 -----
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166
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167 **Extract data from already uploaded SRA dataset**
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168
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169 If a SRA dataset is present in the history, it can be converted into BAM dataset by setting **select input type** drop-down to *SRA archive in current history*. Just like in the case of extracting data for single accession number a single BAM dataset will be generated in the history.
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170
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171 @ACCESSION_LIST_HOWTO@
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172
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173 -----
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174
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175 .. _BAM: https://samtools.github.io/hts-specs/SAMv1.pdf
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176 .. _sam-dump: http://ncbi.github.io/sra-tools/sam-dump.html
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177 .. _collection: https://galaxyproject.org/tutorials/collections/
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178 .. _link: http://www.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?view=studies
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179
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180 @SRATOOLS_ATTRRIBUTION@
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181 ]]></help>
0
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182 <expand macro="citation"/>
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183 </tool>