annotate quast.xml @ 14:459ceb2ff536 draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
author iuc
date Fri, 25 Mar 2022 17:36:16 +0000
parents 74be236ac5fd
children 25368452f73b
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rev   line source
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1 <tool id="quast" name="Quast" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="20.01">
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2 <description>Genome assembly Quality</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="bio_tools"/>
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7 <expand macro='requirements' />
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8 <command detect_errors="exit_code">
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9 <![CDATA[
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10 #import re
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11 #import os
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12
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13 #if str($in.custom) == 'false'
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14 #set $labels = ','.join( [re.sub('[^\w\-_]', '_', str($x.element_identifier)) for $x in $in.inputs])
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15 echo $labels &&
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16 #else
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17 #set $labels = []
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18 #for $x in $in.inputs
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19 #if str($x.labels) != ''
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20 #silent $labels.append(re.sub('[^\w\-_]', '_', str($x.labels)))
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21 #else
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22 #silent $labels.append(re.sub('[^\w\-_]', '_', str($x.input.element_identifier)))
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23 #end if
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24 #end for
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25 #set $labels = ','.join($labels)
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26 #end if
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28 #if $assembly.type == 'metagenome' and $assembly.ref.origin == 'list'
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29 #set $temp_ref_list_fp = 'temp_ref_list_fp'
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30 #set $temp_ref_list_f = open($temp_ref_list_fp, 'w')
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31 #silent $temp_ref_list_f.write('\n'.join([x.strip() for x in $assembly.ref.references_list.split(',')]))
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32 #silent $temp_ref_list_f.close()
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33 #end if
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35 #if $assembly.type == 'genome'
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36 quast
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37 #else
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38 metaquast
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39 #end if
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41 #if $reads.reads_option == 'single'
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42 #for $read in $reads.input_1
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43 --single '$read'
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44 #end for
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45 #else if $reads.reads_option == 'paired'
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46 #for $read in $reads.input_1
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47 --pe1 '$read'
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48 #end for
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49 #for $read in $reads.input_2
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50 --pe2 '$read'
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51 #end for
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52 #else if $reads.reads_option == 'paired_interlaced'
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53 #for $read in $reads.input_1
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54 --pe12 '$read'
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55 #end for
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56 #else if $reads.reads_option == 'mate_paired'
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57 #for $read in $reads.input_1
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58 --mp1 '$read'
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59 #end for
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60 #for $read in $reads.input_2
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61 --mp2 '$read'
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62 #end for
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63 #else if $reads.reads_option == 'pacbio'
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64 #for $read in $reads.input_1
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65 --pacbio '$read'
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66 #end for
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67 #else if $reads.reads_option == 'nanopore'
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68 #for $read in $reads.input_1
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69 --nanopore '$read'
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70 #end for
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71 #end if
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72
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73 --labels '$labels'
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74 -o 'outputdir'
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75
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76 #if $assembly.type == 'genome'
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77 #if $assembly.ref.use_ref == 'true'
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78 -r '$assembly.ref.r'
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79 #if $assembly.ref.features
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80 --features '$assembly.ref.features'
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81 #end if
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82 #if $assembly.ref.operons
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83 --operons '$assembly.ref.operons'
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84 #end if
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85 $assembly.ref.circos
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86 $assembly.ref.k_mer.k_mer_stats
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87 #if str($assembly.ref.k_mer.k_mer_stats) != ''
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88 --k-mer-size $assembly.ref.k_mer.k_mer_size
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89 #end if
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90 #else if $assembly.ref.est_ref_size
10
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91 --est-ref-size $assembly.ref.est_ref_size
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92 #end if
6
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93 $assembly.orga_type
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94 #else
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95 #if $assembly.ref.origin == 'history'
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96 -r '$assembly.ref.r'
6
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97 #else if $assembly.ref.origin == 'list'
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98 --references-list '$temp_ref_list_fp'
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99 #else if $assembly.ref.origin == 'silva'
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100 --test-no-ref
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101 --max-ref-num $assembly.ref.max_ref_num
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102 #end if
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103 #end if
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104
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105 --min-contig $min_contig
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106 $split_scaffolds
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107 $large
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108
6
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109 #if str($genes.gene_finding.tool) != 'none'
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110 $genes.gene_finding.tool
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111 #if $genes.gene_finding.tool == '--gene_finding' or $genes.gene_finding.tool == '--glimmer'
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112 #set $gene_threshold = ','.join([x.strip() for x in str($genes.gene_finding.gene_thresholds).split(',')])
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113 --gene-thresholds '$gene_threshold'
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114 #end if
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115 #end if
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116
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117 $genes.rna_finding
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118 $genes.conserved_genes_finding
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119 $alignments.use_all_alignments
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120 --min-alignment $alignments.min_alignment
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121 --min-identity $alignments.min_identity
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122 --ambiguity-usage '$alignments.ambiguity_usage'
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123 --ambiguity-score $alignments.ambiguity_score
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124 $alignments.fragmented
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125 $alignments.upper_bound_assembly
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126 #if $alignments.upper_bound_min_con
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127 --upper-bound-min-con $alignments.upper_bound_min_con
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128 #end if
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129
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130 #if $alignments.fragmented
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131 #if $advanced.fragmented_max_indent != ''
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132 --fragmented-max-indent $advanced.fragmented_max_indent
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133 #end if
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134 #end if
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135
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136 #set $contig_thresholds = ','.join([x.strip() for x in str($advanced.contig_thresholds).split(',')])
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137 --contig-thresholds '$contig_thresholds'
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138 $advanced.strict_NA
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139 --extensive-mis-size $advanced.extensive_mis_size
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140 --scaffold-gap-max-size $advanced.scaffold_gap_max_size
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141 --unaligned-part-size $advanced.unaligned_part_size
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142 $advanced.skip_unaligned_mis_contigs
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143
6
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144 #if str($in.custom) == 'false'
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145 #for $k in $in.inputs
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146 '$k'
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147 #end for
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148 #else
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149 #for $k in $in.inputs
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150 '$k.input'
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151 #end for
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152 #end if
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153
10
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154 --threads \${GALAXY_SLOTS:-1}
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155
6
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156 && mkdir -p '$report_html.files_path'
5
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157 && cp outputdir/*.html '$report_html.files_path'
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158
6
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159 #if ($assembly.type == 'genome' and $assembly.ref.use_ref) or ($assembly.type == 'metagenome' and $assembly.ref.origin != 'none')
5
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160 && cp -R outputdir/icarus_viewers '$report_html.files_path'
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161 #end if
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162
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163 #if $assembly.type == 'metagenome'
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164 && if [[ -d "outputdir/combined_reference/" ]]; then mkdir -p '$report_html_meta.files_path' && cp outputdir/combined_reference/*.html '$report_html_meta.files_path'; fi
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
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165 #if $assembly.ref.origin != 'none'
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166 && if [[ -d "outputdir/combined_reference/" ]]; then cp -R outputdir/combined_reference/icarus_viewers '$report_html_meta.files_path'; fi
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167 && if [[ -d "outputdir/krona_charts/" ]]; then mkdir -p '$krona.files_path' && cp outputdir/krona_charts/*.html '$krona.files_path'; fi
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
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168 #end if
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169 #end if
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170
0
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171 ]]>
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172 </command>
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173 <inputs>
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174 <conditional name="in">
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175 <param name="custom" type="select" label="Use customized names for the input files?" help="They will be used in reports, plots and logs">
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176 <option value="true">Yes, specify custom names</option>
6
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177 <option value="false" selected="true">No, use dataset names</option>
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178 </param>
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179 <when value="true">
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180 <repeat name="inputs" title="Contigs/scaffolds" min="1">
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181 <param name="input" type="data" format="fasta" label="Contigs/scaffolds file"/>
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182 <param argument="--labels" type="text" value="" label="Name"/>
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183 </repeat>
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184 </when>
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185 <when value="false">
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186 <param name="inputs" type="data" format="fasta" multiple="true" label="Contigs/scaffolds file"/>
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187 </when>
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188 </conditional>
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189 <conditional name="reads">
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190 <param name="reads_option" type="select" label="Reads options" help="Currently, the supported read types are Illumina unpaired, paired-end and mate-pair reads, PacBio SMRT, and Oxford Nanopore long reads.">
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191 <option value="disabled">Disabled</option>
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192 <option value="single">Illumina single-end reads</option>
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193 <option value="paired">Illumina paired-end reads</option>
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194 <option value="paired_interlaced">Illumina interlaced paired-end reads</option>
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195 <option value="mate_paired">Illumina mate-pair reads</option>
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196 <option value="pacbio">Pacbio SMRT reads</option>
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197 <option value="nanopore">Nanopore reads</option>
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198 </param>
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199 <when value="disabled"/>
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200 <when value="single">
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201 <param name="input_1" format="fastq,fastq.gz,fasta,fasta.gz" type="data" multiple="true" label="FASTQ/FASTA file" />
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202 </when>
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203 <when value="paired">
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204 <param name="input_1" format="fastq,fastq.gz,fasta,fasta.gz" type="data" multiple="true" label="FASTQ/FASTA file #1" />
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205 <param name="input_2" format="fastq,fastq.gz,fasta,fasta.gz" type="data" multiple="true" label="FASTQ/FASTA file #2" />
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206 </when>
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207
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208 <when value="paired_interlaced">
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209 <param name="input_1" format="fastq,fastq.gz,fasta,fasta.gz" type="data" multiple="true" label="FASTQ/FASTA file" />
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210 </when>
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211 <when value="mate_paired">
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212 <param name="input_1" format="fastq,fastq.gz,fasta,fasta.gz" type="data" multiple="true" label="FASTQ/FASTA file #1" />
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213 <param name="input_2" format="fastq,fastq.gz,fasta,fasta.gz" type="data" multiple="true" label="FASTQ/FASTA file #2" />
10
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214 </when>
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215 <when value="pacbio">
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216 <param name="input_1" format="fastq,fastq.gz,fasta,fasta.gz" type="data" multiple="true" label="FASTQ/FASTA file" />
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217 </when>
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218 <when value="nanopore">
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219 <param name="input_1" format="fastq,fastq.gz,fasta,fasta.gz" type="data" multiple="true" label="FASTQ/FASTA file" />
10
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220 </when>
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221 </conditional>
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222 <conditional name="assembly">
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223 <param name="type" type="select" label="Type of assembly">
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224 <option value="genome">Genome</option>
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225 <option value="metagenome">Metagenome</option>
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226 </param>
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227 <when value="genome">
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228 <conditional name="ref">
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229 <param name="use_ref" type="select" label="Use a reference genome?" help="Many metrics can't be evaluated without a reference. If this is omitted, QUAST will only report the metrics that can be evaluated without a reference.">
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230 <option value="true">Yes</option>
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231 <option value="false" selected="true">No</option>
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232 </param>
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233 <when value="true">
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234 <param argument="-r" type="data" format="fasta" multiple="true" label="Reference genome" />
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235 <param argument="--features" type="data" format="gff, gff3, bed" optional="true" label="Genomic feature positions in the reference genome" help="Gene coordinates for the reference genome"/>
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236 <param argument="--operons" type="data" format="gff, gff3, bed" optional="true" label="Operon positions in the reference genome" help="Operon coordinates for the reference genome"/>
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237 <conditional name="k_mer">
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238 <param argument="--k-mer-stats" type="select" label="Compute k-mer-based quality metrics?" help="It is recommended for large genomes. This may significantly increase memory and time consumption on large genomes">
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239 <option value="--k-mer-stats">Yes</option>
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240 <option value="" selected="true">No</option>
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241 </param>
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242 <when value="--k-mer-stats">
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243 <param name="k_mer_size" argument="--k-mer-size" type="integer" value="101" label="Size of k" />
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244 </when>
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245 <when value=""/>
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246 </conditional>
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247 <param argument="--circos" type="boolean" truevalue="--circos" falsevalue="" checked="false" label="Generage Circos plot" help="Plot Circos version of Icarus contig alignment viewer"/>
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248 </when>
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249 <when value="false">
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250 <param argument="--est-ref-size" type="integer" optional="true" label="Estimated reference genome size (in bp) for computing NGx statistics" help=""/>
6
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251 </when>
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252 </conditional>
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253 <param name="orga_type" type="select" label="Type of organism">
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254 <option value="">Prokaryotes: use of GeneMarkS for gene finding (default)</option>
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255 <option value="--eukaryote">Eukaryote: use of GeneMark-ES for gene finding, Barrnap for ribosomal RNA genes prediction, BUSCO for conserved orthologs finding (--eukaryote)</option>
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256 <option value="--fungus">Fungus: use of GeneMark-ES for gene finding, Barrnap for ribosomal RNA genes prediction, BUSCO for conserved orthologs finding (--fungus)</option>
6
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257 </param>
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258 </when>
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259 <when value="metagenome">
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260 <conditional name="ref">
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261 <param name="origin" type="select" label="Reference genome" help="Many metrics can't be evaluated without a reference. If this is omitted, QUAST will only report the metrics that can be evaluated without a reference.">
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262 <option value="history">From history</option>
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263 <option value="list">From list</option>
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264 <option value="silva">From SILVA database</option>
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265 <option value="none" selected="true">None</option>
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266 </param>
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267 <when value="history">
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268 <param argument="-r" type="data" format="fasta" multiple="true" label="Reference genome" />
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269 </when>
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270 <when value="list">
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271 <param name="references_list" argument="references-list" type="text" value="" label="Comma-separated list of reference genomes" help="MetaQUAST will search for these references in the NCBI database and will download the found ones"/>
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272 </when>
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273 <when value="silva">
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274 <param name="max_ref_num" argument="-max-ref-num" type="integer" value="50" label="Maximum number of reference genomes (per each assembly) to download after searching in the SILVA databa" />
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275 </when>
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276 <when value="none"/>
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277 </conditional>
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278 </when>
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279 </conditional>
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280 <param argument="--min-contig" type="integer" value="500" label="Lower threshold for a contig length (in bp)" help="Shorter contigs won't be taken into account"/>
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281 <param argument="--split-scaffolds" type="boolean" truevalue="--split-scaffolds" falsevalue="" checked="false" label="Are assemblies scaffolds rather than contigs?" help="QUAST will add split versions of assemblies to the comparison. Assemblies are split by continuous fragments of N's of length >= 10. If broken version is equal to the original assembly (i.e. nothing was split) it is not included in the comparison."/>
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282 <param argument="--large" type="boolean" truevalue="--large" falsevalue="" checked="false" label="Is genome large (> 100 Mbp)?" help="Use optimal parameters for evaluation of large genomes. Affects speed and accuracy. In particular, imposes --eukaryote --min-contig 3000 --min-alignment 500 --extensive-mis-size 7000 (can be overridden manually with the corresponding options). In addition, this mode tries to identify misassemblies caused by transposable elements and exclude them from the number of misassemblies."/>
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283 <section name="genes" title="Genes">
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284 <conditional name="gene_finding">
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285 <param name="tool" type="select" label="Tool for gene prediction" help="">
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286 <option value="none">Don't predict genes</option>
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287 <option value="--gene-finding">GeneMarkS if prokaryotes or GeneMark-ES if eukaryotes or fungi</option>
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288 <option value="--mgm">MetaGeneMark, specially for metagenomic assembly</option>
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289 <option value="--glimmer">Glimmer</option>
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290 </param>
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291 <when value="none"/>
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292 <when value="--gene-finding">
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293 <expand macro="gene_thresholds"/>
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294 </when>
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295 <when value="--mgm"/>
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296 <when value="--glimmer">
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297 <expand macro="gene_thresholds"/>
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298 </when>
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299 </conditional>
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300 <param argument="--rna-finding" type="boolean" truevalue="--rna-finding" falsevalue="" checked="false" label="Enables ribosomal RNA gene finding?" help="By default, we assume that the genome is prokaryotic, and Barrnap uses the bacterial database for rRNA prediction. If the genome is eukaryotic (fungal), use --eukaryote (--fungus) option to force Barrnap to work with the eukaryotic (fungal) database. "/>
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301 <param argument="--conserved-genes-finding" type="boolean" truevalue="--conserved-genes-finding" falsevalue="" checked="false" label="Enables search for Universal Single-Copy Orthologs using BUSCO?" help="By default, we assume that the genome is prokaryotic, and BUSCO uses the bacterial database of orthologs. If the genome is eukaryotic (fungal), use --eukaryote (--fungus) option to force BUSCO to work with the eukaryotic (fungal) database. "/>
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302 </section>
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303 <section name="alignments" title="Alignments">
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304 <param argument="--use-all-alignments" type="boolean" truevalue="--use-all-alignments" falsevalue="" checked="false" label="Use all alignments as in QUAST v.1.*. to compute genome fraction, # genomic features, # operons metrics?" help="By default, QUAST v.2.0 and higher filters out ambiguous and redundant alignments, keeping only one alignment per contig (or one set of non-overlapping or slightly overlapping alignments)"/>
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305 <param argument="--min-alignment" type="integer" value="65" label="Minimum length of alignment" help="Alignments shorter than this value will be filtered. Note that all alignments shorter than 65 bp will be filtered regardless of this threshold."/>
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306 <param argument="--min-identity" type="float" value="95.0" label="Minimum IDY% considered as proper alignment" help="Alignments with IDY% worse than this value will be filtered. ote that all alignments with IDY% less than 80.0% will be filtered regardless of this threshold. "/>
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307 <param argument="--ambiguity-usage" type="select" label="How processing equally good alignments of a contig (probably repeats)?" help="">
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308 <option value="none">Skip all such alignments</option>
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309 <option value="one" selected="true">Take only one (the very best one)</option>
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310 <option value="all">Use all alignments. It can cause a significant increase of # mismatches (repeats are almost always inexact due to accumulated SNPs, indels, etc.). It is useful for metagenomic assemblies where ambiguous alignments might represent homologous sequences of different strains</option>
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311 </param>
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312 <param argument="--ambiguity-score" type="float" value="0.99" min="0.8" max="1.0" label="Score S for defining equally good alignments of a single contig" help="All alignments are sorted by decreasing LEN × IDY% value. All alignments with LEN × IDY% less than S × best(LEN × IDY%) are discarded. "/>
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313 <param argument="--fragmented" type="boolean" truevalue="--fragmented" falsevalue="" checked="false" label="Fragmented reference genome" help="Reference genome is fragmented (e.g. a scaffold reference). QUAST will try to detect misassemblies caused by the fragmentation and mark them fake (will be excluded from misassemblies). Note: QUAST will not detect misassemblies caused by the linear representation of circular genome "/>
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314 <param argument="--upper-bound-assembly" type="boolean" truevalue="--upper-bound-assembly" falsevalue="" label="Simulate upper bound assembly" help="Simulate upper bound assembly based on the reference genome and a given set reads (mate-pairs or long reads, such as Pacbio SMRT/Oxford Nanopore, are REQUIRED). This assembly is added to the comparison and could be useful for estimating the upper bounds of completeness and contiguity that theoretically can be reached by assembly software from this particular set of reads. The concept is based on the fact that the reference genome cannot be completely reconstructed from raw reads due to long genomic repeats and low covered regions." />
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315 <param argument="--upper-bound-min-con" type="integer" value="" optional="true" label="Minimal number of 'connecting reads' needed for joining upper bound contigs into a scaffold" help="This is important for a realistic estimation of genome assembly fragmentation due to long repeats. The default values is 2 for mate-pairs and 1 for long reads (PacBio or Nanopore libraries)"/>
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316 </section>
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317 <section name="advanced" title="Advanced options">
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318 <param argument="--contig-thresholds" type="text" value="0,1000" label="Comma-separated list of contig length thresholds (in bp)" help="Used in # contigs ≥ x and total length (≥ x) metrics"/>
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319 <param argument="--strict-NA" type="boolean" truevalue="--strict-NA" falsevalue="" checked="false" label="Break contigs at every misassembly event (including local ones) to compute NAx and NGAx statistics?" help="By default, QUAST breaks contigs only at extensive misassemblies (not local ones)."/>
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320 <param argument="--extensive-mis-size" type="integer" value="1000" min="85" label="Lower threshold for the relocation size (gap or overlap size between left and right flanking sequence)" help="Shorter relocations are considered as local misassemblies. It does not affect other types of extensive misassemblies (inversions and translocations). The default value is 1000 bp. Note that the threshold should be greater than maximum indel length which is 85 bp."/>
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321 <param argument="--scaffold-gap-max-size" type="integer" value="1000" label="Max allowed scaffold gap length difference for detecting corresponding type of misassemblies" help="Longer inconsistencies are considered as relocations and thus, counted as extensive misassemblies. The default value is 10000 bp. Note that the threshold make sense only if it is greater than extensive misassembly size"/>
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322 <param argument="--unaligned-part-size" type="integer" value="500" label="Lower threshold for detecting partially unaligned contigs" help=""/>
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323 <param argument="--skip-unaligned-mis-contigs" type="boolean" truevalue="" falsevalue="--skip-unaligned-mis-contigs" checked="true" label="Distinguish contigs with more than 50% unaligned bases as a separate group of contigs?" help="By default, QUAST breaks contigs only at extensive misassemblies (not local ones)."/>
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324 <param argument="--fragmented-max-indent" type="integer" min="0" value="" optional="true" label="Fragment max indent" help="Mark translocation as fake if both alignments are located no further than N bases from the ends of the reference fragments. The value should be less than extensive misassembly size.Default value is 50. Note: requires --fragmented option" />
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325 </section>
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326 <param name="output_files" type="select" display="checkboxes" optional="true" multiple="true" label="Output files">
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327 <option value="html" selected="true">HTML reports</option>
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328 <option value="pdf">PDF reports</option>
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329 <option value="tabular">Tabular reports</option>
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330 <option value="log">Log file</option>
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331 <option value="summary">Key metric summary (metagenome mode)</option>
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332 <option value="krona">Krona charts (metagenome mode without reference genomes)</option>
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333 </param>
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334 </inputs>
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335 <outputs>
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336 <data name="report_tabular" format="tabular" label="${tool.name} on ${on_string}: tabular report" from_work_dir="outputdir/report.tsv">
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337 <filter>assembly['type'] == 'genome' and 'tabular' in output_files</filter>
10
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338 </data>
14
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339 <data name="report_tabular_meta" format="tabular" label="${tool.name} on ${on_string}: tabular report for combined reference genome" from_work_dir="outputdir/combined_reference/report.tsv">
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340 <filter>assembly['type'] == 'metagenome' and 'tabular' in output_files</filter>
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341 </data>
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342 <data name="report_html" format="html" label="${tool.name} on ${on_string}: HTML report" from_work_dir="outputdir/report.html">
10
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343 <filter>'html' in output_files</filter>
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344 </data>
14
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345 <data name="report_html_meta" format="html" label="${tool.name} on ${on_string}: HTML report for combined reference genome" from_work_dir="outputdir/combined_reference/report.html">
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346 <filter>assembly['type'] == 'metagenome' and 'html' in output_files</filter>
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347 </data>
10
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348 <data name="report_pdf" format="pdf" label="${tool.name} on ${on_string}: PDF report" from_work_dir="outputdir/report.pdf">
14
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349 <filter>assembly['type'] == 'genome' and 'pdf' in output_files</filter>
10
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350 </data>
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351 <data name="log" format="txt" label="${tool.name} on ${on_string}: Log" from_work_dir="outputdir/quast.log">
14
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352 <filter>assembly['type'] == 'genome' and 'log' in output_files</filter>
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353 </data>
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354 <data name="log_meta" format="txt" label="${tool.name} on ${on_string}: Log" from_work_dir="outputdir/metaquast.log">
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355 <filter>assembly['type'] == 'metagenome' and 'log' in output_files</filter>
4
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parents: 3
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356 </data>
10
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357 <data name="mis_ass" format="tabular" label="${tool.name} on ${on_string}: Misassemblies report" from_work_dir="outputdir/contigs_reports/misassemblies_report.txt">
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358 <filter>assembly['type'] == 'genome' and assembly['ref']['use_ref'] == 'true'</filter>
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359 <filter>'tabular' in output_files</filter>
4
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parents: 3
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360 </data>
10
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361 <data name="unalign" format="tabular" label="${tool.name} on ${on_string}: Unaligned contigs report" from_work_dir="outputdir/contigs_reports/unaligned_report.tsv">
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362 <filter>assembly['type'] == 'genome' and assembly['ref']['use_ref'] == 'true'</filter>
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363 <filter>'tabular' in output_files</filter>
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364 </data>
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365 <data name="kmers" format="tabular" label="${tool.name} on ${on_string}: K-mer-based metrics report" from_work_dir="outputdir/k_mer_stats/kmers_report.txt">
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366 <filter>assembly['type'] == 'genome' and assembly['ref']['use_ref'] == 'true' and assembly['ref']['k_mer']['k_mer_stats'] != ''</filter>
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367 <filter>'tabular' in output_files</filter>
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368 </data>
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369 <data name="circos_output" format="png" from_work_dir="outputdir/circos/circos.png" label="${tool.name} on ${on_string}: Circos plot">
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370 <filter>assembly['type'] == 'genome' and assembly['ref']['use_ref'] == 'true' and assembly['ref']['circos']</filter>
6
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parents: 5
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371 </data>
14
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372 <collection name="metrics_tabular" type="list" label="${tool.name} on ${on_string}: Tabular reports for key metrics" >
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373 <discover_datasets pattern="(?P&lt;designation&gt;.+).tsv" directory="outputdir/summary/TSV/" format="tabular"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
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374 <filter>assembly['type'] == 'metagenome' and 'summary' in output_files</filter>
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375 </collection>
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376 <collection name="metrics_pdf" type="list" label="${tool.name} on ${on_string}: PDF reports for key metrics" >
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
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parents: 13
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377 <discover_datasets pattern="(?P&lt;designation&gt;.+).pdf" directory="outputdir/summary/PDF/" format="pdf"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
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378 <filter>assembly['type'] == 'metagenome' and 'summary' in output_files</filter>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
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379 </collection>
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380 <data name="krona" format="html" label="${tool.name} on ${on_string}: Krona chart" from_work_dir="outputdir/krona_charts/*.html">
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381 <filter>assembly['type'] == 'metagenome' and assembly['ref']['origin'] == 'none' and 'krona' in output_files</filter>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
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parents: 13
diff changeset
382 </data>
0
350257634bbb planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit d0e7095e03f99c80d6cc8f7a2bfb3c5bef33df09
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parents:
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383 </outputs>
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diff changeset
384 <tests>
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385 <!-- Test 01: reference, genes annotations and operon coordinates -->
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386 <test expect_num_outputs="2">
6
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parents: 5
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387 <conditional name="in">
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388 <param name="custom" value="true"/>
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389 <repeat name="inputs">
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390 <param name="input" value="contigs1.fna"/>
bf2c7b30dfb6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 911441d53ac3a7a229c448d66101bcd9179e771b
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391 <param name="labels" value="contig1"/>
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parents: 5
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392 </repeat>
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parents: 5
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393 <repeat name="inputs">
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394 <param name="input" value="contigs2.fna"/>
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395 <param name="labels" value="contig2"/>
6
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396 </repeat>
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397 </conditional>
bf2c7b30dfb6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 911441d53ac3a7a229c448d66101bcd9179e771b
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parents: 5
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398 <conditional name="assembly">
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399 <param name="type" value="genome"/>
bf2c7b30dfb6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 911441d53ac3a7a229c448d66101bcd9179e771b
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parents: 5
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400 <conditional name="ref">
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401 <param name="use_ref" value="true"/>
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402 <param name="r" value="reference.fna"/>
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403 <param name="features" value="genes.gff"/>
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404 <param name="operons" value="operons.bed"/>
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
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parents: 9
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405 <conditional name="k_mer">
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
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406 <param name="k_mer_stats" value="--k-mer-stats"/>
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
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407 <param name="k_mer_size" value="101" />
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408 </conditional>
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409 <param name="circos" value="true"/>
6
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parents: 5
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410 </conditional>
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parents: 5
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411 <param name="orga_type" value=""/>
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parents: 5
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412 </conditional>
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413 <param name="min_contig" value="500"/>
bf2c7b30dfb6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 911441d53ac3a7a229c448d66101bcd9179e771b
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parents: 5
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414 <param name="split_scaffolds" value="false"/>
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parents: 5
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415 <section name="genes">
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parents: 5
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416 <conditional name="gene_finding">
bf2c7b30dfb6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 911441d53ac3a7a229c448d66101bcd9179e771b
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417 <param name="tool" value="--gene_finding"/>
bf2c7b30dfb6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 911441d53ac3a7a229c448d66101bcd9179e771b
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418 <param name="gene_thresholds" value="0,300,1500,3000"/>
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419 </conditional>
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420 <param name="rna_finding" value="true"/>
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421 <param name="conserved_genes_finding" value="true"/>
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422 </section>
10
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423 <section name="alignments">
6
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424 <param name="use_all_alignments" value="true"/>
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425 <param name="min_alignment" value="65"/>
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426 <param name="min_identity" value="95.0"/>
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427 <param name="ambiguity_usage" value="one"/>
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428 <param name="ambiguity_score" value="0.99"/>
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429 </section>
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430 <section name="advanced">
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431 <param name="contig_thresholds" value="0,1000"/>
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432 <param name="strict_NA" value="true"/>
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433 <param name="extensive_mis_size" value="1000"/>
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434 <param name="scaffold_gap_max_size" value="1000"/>
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435 <param name="unaligned_part_size" value="500"/>
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436 <param name="skip_unaligned_mis_contigs" value="true"/>
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437 <param name="fragmented_max_indent" value="50"/>
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438 </section>
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439 <param name="output_files" value="html"/>
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440 <output name="report_html" file="test1_report.html" ftype="html" compare="sim_size"/>
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441 <output name="circos_output" file="test1_circos.png" ftype="png" compare="sim_size"/>
0
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442 </test>
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443 <!-- Test 02: all outputs -->
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444 <test expect_num_outputs="8">
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445 <conditional name="in">
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446 <param name="custom" value="true"/>
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447 <repeat name="inputs">
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448 <param name="input" value="contigs1.fna"/>
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449 <param name="labels" value="contig1"/>
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450 </repeat>
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451 <repeat name="inputs">
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452 <param name="input" value="contigs2.fna"/>
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453 <param name="labels" value="contig2"/>
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454 </repeat>
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455 </conditional>
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456 <conditional name="assembly">
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457 <param name="type" value="genome"/>
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458 <conditional name="ref">
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459 <param name="use_ref" value="true"/>
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460 <param name="r" value="reference.fna"/>
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461 <param name="features" value="genes.gff"/>
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462 <param name="operons" value="operons.bed"/>
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463 <conditional name="k_mer">
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464 <param name="k_mer_stats" value="--k-mer-stats"/>
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465 <param name="k_mer_size" value="101" />
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466 </conditional>
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467 <param name="circos" value="true"/>
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468 </conditional>
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469 </conditional>
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470 <param name="output_files" value="html,pdf,tabular,log"/>
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471 <output name="report_html" file="test2_report.html" ftype="html" compare="sim_size"/>
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472 <output name="report_pdf" file="test2_report.pdf" ftype="pdf" compare="sim_size"/>
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473 <output name="report_tabular" file="test2_report.tab" ftype="tabular"/>
10
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474 <output name="log" file="test2.log" ftype="txt" compare="sim_size"/>
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475 <output name="mis_ass" file="test2_missasemblies.tab" ftype="tabular"/>
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476 <output name="unalign" file="test2_unaligned.tab" ftype="tabular"/>
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477 <output name="kmers" file="test2_kmers.tab" ftype="tabular"/>
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478 <output name="circos_output" file="test2_circos.png" ftype="png" compare="sim_size"/>
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479 </test>
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480 <!-- Test 03: without reference -->
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481 <test expect_num_outputs="3">
6
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482 <conditional name="in">
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483 <param name="custom" value="false"/>
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484 <param name="inputs" value="contigs1.fna,contigs2.fna"/>
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485 </conditional>
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486 <conditional name="assembly">
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487 <param name="type" value="genome"/>
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488 <conditional name="ref">
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489 <param name="use_ref" value="false"/>
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490 </conditional>
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491 <param name="orga_type" value="--eukaryote"/>
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492 </conditional>
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493 <param name="min_contig" value="500"/>
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494 <param name="split_scaffolds" value="false"/>
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495 <param name="large" value="false"/>
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496 <section name="genes">
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497 <conditional name="gene_finding">
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498 <param name="tool" value="none"/>
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499 </conditional>
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500 <param name="rna_finding" value="false"/>
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501 <param name="conserved_genes_finding" value="false"/>
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502 </section>
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503 <section name="alignments">
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504 <param name="use_all_alignments" value="false"/>
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505 <param name="min_alignment" value="65"/>
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506 <param name="min_identity" value="95.0"/>
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507 <param name="ambiguity_usage" value="one"/>
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508 <param name="ambiguity_score" value="0.99"/>
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509 <param name="fragmented" value="false"/>
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510 </section>
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511 <section name="advanced">
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512 <param name="contig_thresholds" value="0,1000, 500"/>
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513 <param name="strict_NA" value="false"/>
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514 <param name="extensive_mis_size" value="1000"/>
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515 <param name="scaffold_gap_max_size" value="1000"/>
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516 <param name="unaligned_part_size" value="500"/>
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517 <param name="skip_unaligned_mis_contigs" value="-"/>
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518 </section>
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519 <param name="output_files" value="html,pdf,log" />
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520 <output name="log" file="test3.log" ftype="txt" compare="sim_size"/>
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521 <output name="report_html" file="test3_report.html" compare="sim_size"/>
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522 <output name="report_pdf" file="test3_report.pdf" compare="sim_size"/>
3
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523 </test>
10
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524 <!-- Test 04: metagenomics -->
6
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525 <test>
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526 <conditional name="in">
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527 <param name="custom" value="false"/>
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528 <param name="inputs" value="contigs3.fasta"/>
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529 </conditional>
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530 <conditional name="assembly">
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531 <param name="type" value="metagenome"/>
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532 <conditional name="ref">
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533 <param name="origin" value="none"/>
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534 </conditional>
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535 </conditional>
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536 <param name="min_contig" value="500"/>
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537 <param name="split_scaffolds" value="false"/>
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538 <param name="large" value="false"/>
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539 <section name="genes">
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540 <conditional name="gene_finding">
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541 <param name="tool" value="--mgm"/>
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542 </conditional>
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543 <param name="rna_finding" value="false"/>
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544 <param name="conserved_genes_finding" value="false"/>
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545 </section>
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546 <section name="alignments">
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547 <param name="use_all_alignments" value="false"/>
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548 <param name="min_alignment" value="65"/>
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549 <param name="min_identity" value="95.0"/>
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550 <param name="ambiguity_usage" value="one"/>
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diff changeset
551 <param name="ambiguity_score" value="0.99"/>
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552 <param name="fragmented" value="false"/>
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553 </section>
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554 <section name="advanced">
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555 <param name="contig_thresholds" value="0,1000, 500"/>
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556 <param name="strict_NA" value="false"/>
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557 <param name="extensive_mis_size" value="1000"/>
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558 <param name="scaffold_gap_max_size" value="1000"/>
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diff changeset
559 <param name="unaligned_part_size" value="500"/>
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560 <param name="skip_unaligned_mis_contigs" value="-"/>
6
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561 </section>
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diff changeset
562 <param name="output_files" value="log"/>
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563 <output name="log_meta" ftype="txt">
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564 <assert_contents>
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diff changeset
565 <has_text text="Reference genomes are not found" />
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566 </assert_contents>
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diff changeset
567 </output>
10
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diff changeset
568 </test>
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diff changeset
569 <!-- Test 05: FASTQ read files -->
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570 <test expect_num_outputs="3">
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571 <conditional name="in">
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572 <param name="custom" value="true"/>
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diff changeset
573 <repeat name="inputs">
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574 <param name="input" value="contigs1.fna"/>
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diff changeset
575 <param name="labels" value="contig1"/>
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576 </repeat>
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577 <repeat name="inputs">
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578 <param name="input" value="contigs2.fna"/>
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579 <param name="labels" value="contig2"/>
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580 </repeat>
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581 </conditional>
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582 <conditional name="reads">
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583 <param name="reads_option" value="pacbio"/>
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584 <param name="input_1" value="pacbio_01.fastq,pacbio_02.fastq,pacbio_03.fastq,pacbio_04.fastq"/>
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585 </conditional>
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586 <conditional name="assembly">
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diff changeset
587 <param name="type" value="genome"/>
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588 <conditional name="ref">
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diff changeset
589 <param name="use_ref" value="true"/>
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590 <param name="r" value="reference.fna"/>
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diff changeset
591 </conditional>
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592 </conditional>
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diff changeset
593 <section name="alignments">
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diff changeset
594 <param name="upper_bound_assembly" value="true"/>
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595 <param name="upper_bound_min_con" value="1"/>
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diff changeset
596 </section>
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diff changeset
597 <param name="output_files" value="tabular"/>
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diff changeset
598 <output name="report_tabular" file="test5.tab" ftype="tabular"/>
10
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diff changeset
599 <output name="mis_ass" file="test5_missasemblies.tab" ftype="tabular"/>
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
600 <output name="unalign" file="test5_unaligned.tab" ftype="tabular"/>
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
601 </test>
14
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
602 <!-- Test 06: FASTQ.gz read files -->
10
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
603 <test expect_num_outputs="1">
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
604 <conditional name="in">
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
605 <param name="custom" value="true"/>
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
606 <repeat name="inputs">
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
607 <param name="input" value="contigs1.fna"/>
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
608 <param name="labels" value="contig1"/>
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
609 </repeat>
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
610 <repeat name="inputs">
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
611 <param name="input" value="contigs2.fna"/>
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
612 <param name="labels" value="contig2"/>
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
613 </repeat>
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
614 </conditional>
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
615 <conditional name="reads">
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
616 <param name="reads_option" value="single"/>
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
617 <param name="input_1" value="pacbio_01.fastq.gz,pacbio_02.fastq.gz"/>
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
618 </conditional>
e377f054bcd0 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 9e4867db42282a980f1149711159fa811b2d8414"
iuc
parents: 9
diff changeset
619 <param name="output_files" value="tabular"/>
14
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
620 <output name="report_tabular" file="test6.tab" ftype="tabular"/>
6
bf2c7b30dfb6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 911441d53ac3a7a229c448d66101bcd9179e771b
iuc
parents: 5
diff changeset
621 </test>
14
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
622 <!-- Test 07: FASTA.gz read files -->
13
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
623 <test expect_num_outputs="1">
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
624 <conditional name="in">
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
625 <param name="custom" value="true"/>
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
626 <repeat name="inputs">
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
627 <param name="input" value="contigs1.fna"/>
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
628 <param name="labels" value="contig1"/>
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
629 </repeat>
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
630 <repeat name="inputs">
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
631 <param name="input" value="contigs2.fna"/>
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
632 <param name="labels" value="contig2"/>
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
633 </repeat>
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
634 </conditional>
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
635 <conditional name="reads">
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
636 <param name="reads_option" value="single"/>
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
637 <param name="input_1" value="pacbio_01.fasta.gz,pacbio_02.fasta.gz"/>
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
638 </conditional>
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
639 <param name="output_files" value="tabular"/>
14
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
640 <output name="report_tabular" file="test7.tab" ftype="tabular"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
641 </test>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
642 <!-- Test 08: metagenomics all tab outputs-->
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
643 <test expect_num_outputs="3">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
644 <conditional name="in">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
645 <param name="custom" value="false"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
646 <param name="inputs" value="test8.fasta"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
647 </conditional>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
648 <conditional name="assembly">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
649 <param name="type" value="metagenome"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
650 <conditional name="ref">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
651 <param name="origin" value="none"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
652 </conditional>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
653 </conditional>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
654 <param name="min_contig" value="500"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
655 <param name="split_scaffolds" value="false"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
656 <param name="large" value="false"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
657 <section name="genes">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
658 <conditional name="gene_finding">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
659 <param name="tool" value="none"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
660 </conditional>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
661 <param name="rna_finding" value="false"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
662 <param name="conserved_genes_finding" value="false"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
663 </section>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
664 <section name="alignments">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
665 <param name="use_all_alignments" value="false"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
666 <param name="min_alignment" value="65"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
667 <param name="min_identity" value="95.0"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
668 <param name="ambiguity_usage" value="one"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
669 <param name="ambiguity_score" value="0.99"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
670 <param name="fragmented" value="false"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
671 </section>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
672 <section name="advanced">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
673 <param name="contig_thresholds" value="0,1000"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
674 <param name="strict_NA" value="false"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
675 <param name="extensive_mis_size" value="1000"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
676 <param name="scaffold_gap_max_size" value="1000"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
677 <param name="unaligned_part_size" value="500"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
678 <param name="skip_unaligned_mis_contigs" value="-"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
679 </section>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
680 <param name="output_files" value="tabular,summary"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
681 <output name="report_tabular_meta" ftype="tabular">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
682 <assert_contents>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
683 <has_text text="contigs (>= 0 bp)" />
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
684 </assert_contents>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
685 </output>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
686 <output_collection name="metrics_tabular" type="list" count="14"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
687 <output_collection name="metrics_pdf" type="list" count="15"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
688 </test>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
689 <!-- Test 09: metagenomics log, html and krona outputs-->
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
690 <test expect_num_outputs="4">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
691 <conditional name="in">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
692 <param name="custom" value="false"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
693 <param name="inputs" value="test8.fasta"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
694 </conditional>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
695 <conditional name="assembly">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
696 <param name="type" value="metagenome"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
697 <conditional name="ref">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
698 <param name="origin" value="none"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
699 </conditional>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
700 </conditional>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
701 <param name="min_contig" value="500"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
702 <param name="split_scaffolds" value="false"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
703 <param name="large" value="false"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
704 <section name="genes">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
705 <conditional name="gene_finding">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
706 <param name="tool" value="none"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
707 </conditional>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
708 <param name="rna_finding" value="false"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
709 <param name="conserved_genes_finding" value="false"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
710 </section>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
711 <section name="alignments">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
712 <param name="use_all_alignments" value="false"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
713 <param name="min_alignment" value="65"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
714 <param name="min_identity" value="95.0"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
715 <param name="ambiguity_usage" value="all"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
716 <param name="ambiguity_score" value="0.99"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
717 <param name="fragmented" value="false"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
718 </section>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
719 <section name="advanced">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
720 <param name="contig_thresholds" value="0,1000"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
721 <param name="strict_NA" value="false"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
722 <param name="extensive_mis_size" value="1000"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
723 <param name="scaffold_gap_max_size" value="1000"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
724 <param name="unaligned_part_size" value="500"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
725 <param name="skip_unaligned_mis_contigs" value="-"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
726 </section>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
727 <param name="output_files" value="html,log,krona"/>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
728 <output name="report_html" ftype="html">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
729 <assert_contents>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
730 <has_text text="Vibrio_parahaemolyticus" />
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
731 </assert_contents>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
732 </output>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
733 <output name="report_html_meta" ftype="html">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
734 <assert_contents>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
735 <has_text text="Total length (>= 1000 bp)" />
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
736 </assert_contents>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
737 </output>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
738 <output name="log_meta" ftype="txt">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
739 <assert_contents>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
740 <has_text text="Vibrio_parahaemolyticus | successfully downloaded" />
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
741 </assert_contents>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
742 </output>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
743 <output name="krona" ftype="html">
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
744 <assert_contents>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
745 <has_text text="Vibrio_parahaemolyticus" />
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
746 </assert_contents>
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
747 </output>
13
74be236ac5fd "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit f713a7853d074ca39057c680ebadba5cbecf5d86"
iuc
parents: 12
diff changeset
748 </test>
0
350257634bbb planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit d0e7095e03f99c80d6cc8f7a2bfb3c5bef33df09
iuc
parents:
diff changeset
749 </tests>
350257634bbb planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit d0e7095e03f99c80d6cc8f7a2bfb3c5bef33df09
iuc
parents:
diff changeset
750 <help>
350257634bbb planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit d0e7095e03f99c80d6cc8f7a2bfb3c5bef33df09
iuc
parents:
diff changeset
751 <![CDATA[
4
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
752 **What it does**
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
753
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
754 QUAST = QUality ASsessment Tool. The tool evaluates genome assemblies by computing various metrics.
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
755
8
4c2bef52f68e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit e24f6cb40f19bad167b440e119e0300f15e04a78"
iuc
parents: 7
diff changeset
756 If you have one or multiple genome assemblies, you can assess their quality with Quast. It works with or without reference genome. If you are new to Quast, start by reading its `manual page <http://quast.sourceforge.net/docs/manual.html>`_.
4
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
757
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
758 **Using Quast without reference**
0
350257634bbb planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit d0e7095e03f99c80d6cc8f7a2bfb3c5bef33df09
iuc
parents:
diff changeset
759
4
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
760 Without reference Quast can calculate a number of assembly related-metrics but cannot provide any information about potential misassemblies, inversions, translocations, etc. Suppose you have three assemblies produced by Unicycler corresponding to three different antibiotic treatments *car*, *pit*, and *cef* (these stand for carbenicillin, piperacillin, and cefsulodin, respectively). Evaluating them without reference will produce the following Quast outputs:
14
459ceb2ff536 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 73d0b20640827d06320405f8d52cead9bf027beb"
iuc
parents: 13
diff changeset
761
4
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
762 * Quast report in HTML format
8
4c2bef52f68e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit e24f6cb40f19bad167b440e119e0300f15e04a78"
iuc
parents: 7
diff changeset
763 * `Contig viewer <http://quast.sourceforge.net/docs/manual.html#sec3.4>`_ (an HTML file)
4c2bef52f68e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit e24f6cb40f19bad167b440e119e0300f15e04a78"
iuc
parents: 7
diff changeset
764 * `Quast report <http://quast.sourceforge.net/docs/manual.html#sec3.1.1>`_ in Tab-delimited format
4
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
765 * Quast log (a file technical information about Quast tool execution)
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
766
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
767 The **tab delimited Quast report** will contain the following information::
0
350257634bbb planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit d0e7095e03f99c80d6cc8f7a2bfb3c5bef33df09
iuc
parents:
diff changeset
768
4
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
769 Assembly pit_fna cef_fna car_fna
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
770 # contigs (>= 0 bp) 100 91 94
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
771 # contigs (>= 1000 bp) 62 58 61
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
772 Total length (>= 0 bp) 6480635 6481216 6480271
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
773 Total length (>= 1000 bp) 6466917 6468946 6467103
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
774 # contigs 71 66 70
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
775 Largest contig 848753 848766 662053
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
776 Total length 6473173 6474698 6473810
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
777 GC (%) 66.33 66.33 66.33
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
778 N50 270269 289027 254671
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
779 N75 136321 136321 146521
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
780 L50 7 7 8
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
781 L75 15 15 16
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
iuc
parents: 3
diff changeset
782 # N's per 100 kbp 0.00 0.00 0.00
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783
8
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784 where values are defined as specified in `Quast manual <http://quast.sourceforge.net/docs/manual.html#sec3.1.1>`_
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785
4
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786 **Quast report in HTML format** contains graphs in addition to the above metrics, while **Contig viewer** draws contigs ordered from longest to shortest. This ordering is suitable for comparing only largest contigs or number of contigs longer than a specific threshold. The viewer shows N50 and N75 with color and textual indication. If the reference genome is available or at least approximate genome length is known (see `--est-ref-size`), NG50 and NG75 are also shown. You can also tone down contigs shorter than a specified threshold using Icarus control panel:
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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787
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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788 .. image:: $PATH_TO_IMAGES/contig_view_noR.png
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789 :width: 558
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790 :height: 412
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791
8
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792 Also see `Plot description <http://quast.sourceforge.net/docs/manual.html#sec2>`_ section of the manual.
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793
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794 **Using Quast with reference**
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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795
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796 Car, pit, and cef are in fact assemblies of *Pseudomonas aeruginosa* UCBPP-PA14, so we can use its genome as a reference (by supplying a Fasta file containing *P. aeruginosa* pa14 genome to **Reference genome** input box). The following outputs will be produced (note the alignment viewer):
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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797
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798 * Quast report in HTML format
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799 * `Contig viewer <http://quast.sourceforge.net/docs/manual.html#sec3.4>`_ (an HTML file)
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800 * `Alignment viewer <http://quast.sourceforge.net/docs/manual.html#sec3.4>`_ (an HTML file)
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801 * `Quast report <http://quast.sourceforge.net/docs/manual.html#sec3.1.1>`_ in Tab-delimited format
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802 * Summary of `misassemblies <http://quast.sourceforge.net/docs/manual.html#sec3.1.2>`_
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803 * Summary of `unaligned contigs <http://quast.sourceforge.net/docs/manual.html#sec3.1.3>`_
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804 * Quast log (a file technical information about Quast tool execution)
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805
4
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806 With the reference Quast produces a much more comprehensive set of results::
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807
4
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808 Assembly pit_fna cef_fna car_fna
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809 # contigs (>= 0 bp) 100 91 94
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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810 # contigs (>= 1000 bp) 62 58 61
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811 Total length (>= 0 bp) 6480635 6481216 6480271
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812 Total length (>= 1000 bp) 6466917 6468946 6467103
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813 # contigs 71 66 70
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814 Largest contig 848753 848766 662053
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815 Total length 6473173 6474698 6473810
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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816 Reference length 6537648 6537648 6537648
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817 GC (%) 66.33 66.33 66.33
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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818 Reference GC (%) 66.29 66.29 66.29
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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819 N50 270269 289027 254671
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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820 NG50 270269 289027 254671
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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821 N75 136321 136321 146521
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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822 NG75 136321 136321 136321
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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823 L50 7 7 8
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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824 LG50 7 7 8
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825 L75 15 15 16
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826 LG75 15 15 17
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827 # misassemblies 0 0 0
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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828 # misassembled contigs 0 0 0
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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829 Misassembled contigs length 0 0 0
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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830 # local misassemblies 1 1 2
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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831 # unaligned mis. contigs 0 0 0
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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832 # unaligned contigs 0 + 0 0 + 0 0 + 0
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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833 part part part
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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834 Unaligned length 0 0 0
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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835 Genome fraction (%) 99.015 99.038 99.025
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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836 Duplication ratio 1.000 1.000 1.000
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837 # N's per 100 kbp 0.00 0.00 0.00
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838 # mismatches per 100 kbp 3.82 3.63 3.49
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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839 # indels per 100 kbp 1.19 1.13 1.13
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840 Largest alignment 848753 848766 662053
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841 Total aligned length 6473163 6474660 6473792
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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842 NA50 270269 289027 254671
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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843 NGA50 270269 289027 254671
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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844 NA75 136321 136321 146521
d90201633fb9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/quast commit 2c56b5f07500507dad503aab6ec0619ec37f8b87
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845 NGA75 136321 136321 136321
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846 LA50 7 7 8
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847 LGA50 7 7 8
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848 LA75 15 15 16
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849 LGA75 15 15 17
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850
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851 where, again, values are defined as specified in `Quast manual <http://quast.sourceforge.net/docs/manual.html#sec3.1.1>`_. You can see that this report includes a variety of data that can only be computer against a reference assembly.
4
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852
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853 Using reference also produces an **Alignment viewer**:
4
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854
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855 .. image:: $PATH_TO_IMAGES/Align_view.png
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856 :width: 515
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857 :height: 395
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858
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859 Alignment viewer highlights regions of interest as, in this case, missassemblies that can potentially point to genome rearrangements (see more `here <http://quast.sourceforge.net/docs/manual.html#sec3.4>`_).
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860
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861 ]]>
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862 </help>
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863 <expand macro="citations"/>
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864 </tool>