Mercurial > repos > iuc > mothur_chimera_ccode

annotate chimera.ccode.xml @ 12:eaec395cb21b draft default tip

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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit dfdd4c590370ecb3a9806314d43eb5668e36a01a"
author iuc
date Thu, 21 Jan 2021 21:44:39 +0000
parents fd378a7b7c99
children
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
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d6b58ab6093a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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1 <tool profile="16.07" id="mothur_chimera_ccode" name="Chimera.ccode" version="@WRAPPER_VERSION@.0">
d6b58ab6093a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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2 <description>Find putative chimeras using ccode</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
d6b58ab6093a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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6 <expand macro="requirements"/>
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7 <expand macro="stdio"/>
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8 <expand macro="version_command"/>
1
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9 <command><![CDATA[
3
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10 @SHELL_OPTIONS@
1
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11
3
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12 ## create symlinks to input datasets
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13 ln -s '$fasta' fasta.dat &&
d3f6d99d7ad7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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14 ln -s '$alignment.reference' alignment.template.dat &&
d3f6d99d7ad7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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15 #if $mask_cond.source2 == "history":
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16 ln -s '$mask_cond.input' mask_cond.mask.dat &&
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17 #end if
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18
3
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19 echo 'chimera.ccode(
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20 fasta=fasta.dat,
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21 reference=alignment.template.dat,
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22 filter=$filter,
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23 #if $mask_cond.source2 == "default":
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24 mask=default,
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25 #elif $mask_cond.source2 == "history":
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26 mask=mask_cond.mask.dat,
d3f6d99d7ad7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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27 #end if
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28 #if int($window) > 0:
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29 window=$window,
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30 #end if
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31 #if int($numwanted) > 0:
d3f6d99d7ad7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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32 numwanted=$numwanted,
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33 #end if
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34 processors='\${GALAXY_SLOTS:-8}'
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35 )'
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36 | sed 's/ //g' ## mothur trips over whitespace
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37 | mothur
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38 | tee mothur.out.log
0
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39 ]]></command>
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40 <inputs>
3
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41 <param argument="fasta" type="data" format="mothur.align" label="fasta - Candiate Aligned Sequences"/>
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42 <conditional name="alignment">
3
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43 <param name="source" type="select" label="Select Reference Template from">
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44 <option value="hist">History</option>
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45 <option value="ref">Cached Reference</option>
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46 </param>
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47 <when value="ref">
3
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48 <param argument="reference" type="select" label="reference - Select an alignment database">
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49 <options from_data_table="mothur_aligndb"/>
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50 </param>
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51 </when>
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52 <when value="hist">
3
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53 <param argument="reference" type="data" format="fasta" label="reference - Reference to align with"/>
0
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54 </when>
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55 </conditional>
3
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56 <param argument="filter" type="boolean" falsevalue="False" truevalue="True" checked="false" label="filter - Apply a 50% soft vertical filter"/>
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57 <conditional name="mask_cond">
0
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58 <param name="source2" type="select" label="Mask option">
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59 <option value="">None</option>
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60 <option value="default">default ecoli mask</option>
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61 <option value="history">From Your History</option>
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62 </param>
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63 <when value="history">
3
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64 <param argument="mask" type="data" format="mothur.filter" label="Mask for the reference file"/>
0
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65 </when>
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66 <when value=""/>
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67 <when value="default"/>
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68 </conditional>
3
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69 <param name="window" type="integer" value="0" label="window - Length of sequence you want in each window analyzed"
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70 help="Recommended window size is between 5 and 20% of your trimmed sequence length. Default is 10% of the sequence length. Default is used if &lt; 1"/>
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71 <param name="numwanted" type="integer" value="20" label="numwanted - Number of sequences compared with each query"
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72 help="uses default if set to 0"/>
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73 <expand macro="param-savelog"/>
0
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74 </inputs>
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75 <outputs>
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76 <expand macro="logfile-output"/>
3
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77 <data name="ccode.chimeras" format="txt" from_work_dir="fasta.*ccode.chimeras" label="${tool.name} on ${on_string}: ccode.chimeras"/>
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78 <data name="ccode.accnos" format="mothur.accnos" from_work_dir="fasta.*ccode.accnos" label="${tool.name} on ${on_string}: ccode.accnos"/>
0
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79 <data name="mapinfo" format="tabular" from_work_dir="*.mapinfo" label="${tool.name} on ${on_string}: mapinfo"/>
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80 </outputs>
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81 <tests>
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82 <test>
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83 <param name="fasta" value="Mock_S280_L001_R1_001_small.trim.contigs.good.align_head"/>
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84 <param name="source" value="hist"/>
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85 <param name="reference" value="HMP_MOCK.v35.align"/>
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86 <output name="ccode.chimeras" ftype="txt">
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87 <assert_contents>
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88 <has_line_matching expression="^Window\tAvgQ\t\(sdQ\)\tAvgR\t\(sdR\)\tRatio\tAnova$"/>
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89 </assert_contents>
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90 </output>
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91 <output name="ccode.accnos" file="Mock_S280_L001_R1_001_small.trim.contigs.good.ccode.accnos" ftype="mothur.accnos"/>
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92 <output name="mapinfo" md5="ef3353b1467fe95a24153513917e6444" ftype="tabular"/>
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93 <param name="savelog" value="true"/>
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94 <expand macro="logfile-test"/>
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95 </test>
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96 <test> <!-- test with default ecoli mask -->
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97 <param name="fasta" value="Mock_S280_L001_R1_001_small.trim.contigs.good.align_head"/>
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98 <param name="source" value="hist"/>
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99 <param name="reference" value="HMP_MOCK.v35.align"/>
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100 <param name="source2" value="default"/>
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101 <param name="window" value="100"/>
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102 <output name="ccode.chimeras" ftype="txt">
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103 <assert_contents>
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104 <has_line_matching expression="^Window\tAvgQ\t\(sdQ\)\tAvgR\t\(sdR\)\tRatio\tAnova$"/>
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105 </assert_contents>
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106 </output>
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107 <output name="ccode.accnos" md5="47ddf86ec36aafd495aa902b3e06ffca" ftype="mothur.accnos"/>
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108 <output name="mapinfo" md5="25221b1e109d9c0a939efb319e7a9896" ftype="tabular"/>
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109 <param name="savelog" value="true"/>
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110 <expand macro="logfile-test"/>
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111 </test>
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112 </tests>
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113 <help><![CDATA[
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114
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115 @MOTHUR_OVERVIEW@
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116
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117 **Command Documentation**
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118
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119 The chimera.ccode_ command identifies putative chimeras using the ccode approach (Chimera and Cross-Over Detection and Evaluation). Ccode_ compares differences in distances, for each word, between query sequence and reference sequences, and reference sequences and themselves.
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120
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121 This method was written using the algorithms described in the paper_ "Evaluating putative chimeric sequences from PCR-amplified products" by Juan M. Gonzalez, Johannes Zimmerman and Cesareo Saiz-Jimenez.
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122
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123 The program can analyze sequences for any required word length. Generally, values of 5-20% of sequence length appear to deliver accurate results, for example, working on 16S rDNA sequences with a full-length of #1500 nt. It should be noted that the use of fragments either too long or too short might result in a reduction of sensitivity.
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124
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125 .. _Ccode: http://www.microextreme.net/downloads.html
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126 .. _paper: http://bioinformatics.oxfordjournals.org/content/21/3/333.full.pdf
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127 .. _chimera.ccode: https://www.mothur.org/wiki/Chimera.ccode
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128
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129 ]]></help>
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130 <expand macro="citations">
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131 <citation type="doi">10.1093/bioinformatics/bti008</citation>
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132 </expand>
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133 </tool>