annotate mitos2.xml @ 3:994e1ce9f4d5 draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
author iuc
date Wed, 01 Sep 2021 07:40:48 +0000
parents 07f0df25bd0c
children 07caa60ff5c4
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1 <tool id="mitos2" name="@MITOS_NAME@" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@">
0
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2 <description>de-novo annotation of metazoan mitochondrial genomes</description>
3
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3 <xrefs>
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4 <xref type='bio.tools'>mitos</xref>
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5 </xrefs>
0
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6 <macros>
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7 <import>macros.xml</import>
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8 <token name="@MITOS_NAME@">MITOS2</token>
2
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9 <token name="@TOOL_VERSION@">2.0.6</token>
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10 <token name="@VERSION_SUFFIX@">1</token>
0
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11 </macros>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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12 <requirements>
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13 <requirement type="package" version="@TOOL_VERSION@">mitos</requirement>
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14 <requirement type="package">zip</requirement>
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15 </requirements>
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16 <version_command>python -c "import mitos; print(mitos.__version__)"</version_command>
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17 <command detect_errors="aggressive"><![CDATA[
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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18 mkdir outdir &&
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19
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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20 runmitos.py
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21 --input '$input'
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22 --code $code
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23 --outdir outdir
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24 --refdir '/'
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25 --refseqver '$refseqver.fields.path'
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26 $linear
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27 #for tpe in ["prot", "trna", "rrna", "intron", "oril", "orih"]
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28 #if not $tpe in str($advanced.featuretypes).split(',')
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29 --$tpe 0
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30 #end if
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31 #end for
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32 --finovl $advanced.finovl
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33 $advanced.best
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34 #set fragovl=float($advanced.fragovl)/100.0
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35 --fragovl $fragovl
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36 --fragfac $advanced.fragfac
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37
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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38 --evalue $advanced_prot.evalue
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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39 #set cutoff=float($advanced_prot.cutoff)/100.0
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40 --cutoff $cutoff
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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41 --clipfac $advanced_prot.clipfac
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42 $advanced_prot.ncbicode
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43 $advanced_prot.alarab
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44 $advanced_prot.oldstst
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45 $advanced_ncrna.locandgloc
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46 --ncev $advanced_ncrna.ncev
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47 $advanced_ncrna.sensitive
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48 --maxtrnaovl $advanced_ncrna.maxtrnaovl
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49 --maxrrnaovl $advanced_ncrna.maxrrnaovl
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50
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51 #if not ("protein_plot" in str($addoutputs).split(',') or "ncRNA_plot" in str($addoutputs).split(',')):
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52 --noplots
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53 #end if
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54
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55 #if "raw" in str($addoutputs).split(','):
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56 && zip -9 -y -r output.zip outdir/ > /dev/null
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57 #end if
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58 ]]></command>
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59 <inputs>
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60 <param argument="--input" label="Sequence" type="data" format="fasta" help="a single sequence in fasta formated sequence">
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61 <options options_filter_attribute="metadata.sequences">
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62 <filter type="add_value" value="1"/>
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63 </options>
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64 </param>
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65 <param argument="--code" label="Genetic code" type="select">
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66 <option value="2">Vertebrate (2)</option>
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67 <option value="4">Mold, Protozoan, Coelenteral (4)</option>
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68 <option value="5">Invertebrate (5)</option>
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69 <option value="9">Echinoderm, Flatworm (9)</option>
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70 <option value="13">Ascidian (13)</option>
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71 <option value="14">Alternative Flatworm (14)</option>
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72 </param>
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73 <param argument="--refseqver" label="Reference data" type="select" help="contact the administrator of this Galaxy instance if you miss reference data">
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74 <options from_data_table="mitos">
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75 <filter type="static_value" value="mitos2" column="2"/>
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76 </options>
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77 <validator message="No reference annotation is available for MITOS2" type="no_options" />
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78 </param>
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79 <param argument="--linear" checked="false" label="Treat sequence as linear" type="boolean" truevalue="--linear" falsevalue=""/>
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80 <param name="addoutputs" type="select" multiple="true" label="Outputs">
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81 <option value="bed" selected="true">BED</option>
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82 <option value="mito" selected="false">mito</option>
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83 <option value="gff" selected="false">GFF file</option>
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84 <option value="seq" selected="false">SEQ</option>
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85 <option value="fas" selected="false">nucleotide FASTA</option>
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86 <option value="faa" selected="false">protein FASTA</option>
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87 <option value="geneorder" selected="false">geneorder</option>
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88 <option value="protein_plot" selected="false">Protein prediction plot</option>
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89 <option value="ncRNA_plot" selected="false">ncRNA prediction plot</option>
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90 <!--<option value="ncRNA_structure_ps_plots" selected="false">ncRNA structure plots - postscript</option>-->
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91 <option value="ncRNA_structure_svg_plots" selected="false">ncRNA structure plots - svg</option>
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92 <option value="raw" selected="false">zipped raw results</option>
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93 </param>
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94 <section name="advanced" title="Advanced options">
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95 <param name="featuretypes" label="Feature types" help="Feature types that should be predicted by MITOS (--noprot,--notrna,--norrna)" type="select" multiple="true">
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96 <option value="prot" selected="true">Protein coding genes</option>
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97 <option value="trna" selected="true">tRNAs</option>
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98 <option value="rrna" selected="true">rRNAs</option>
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99 <option value="intron" selected="false">Introns</option>
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100 <option value="oril" selected="false">Origin of light strand replication</option>
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101 <option value="orih" selected="false">Origin of heavy strand replication</option>
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102 </param>
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103 <param argument="--finovl" label="Final overlap (nt)" help="Maximum number of nucleotides by which genes of different types may overlap" type="integer" value="50" min="0"/>
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104 <param argument="--best" checked="false" label="Annotate only the best copy of each feature" type="boolean" truevalue="--best" falsevalue=""/>
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105 <param argument="--fragovl" label="Fragment overlap" help="Maximum allowed overlap of proteins in the query (in percent of the shorter query range) for two hits to be counted as fragments of the same gene" type="integer" value="20" min="0" max="100"/>
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106 <param argument="--fragfac" label="Fragment quality factor" help="Maximum factor by which fragments of the same protein may differ in their quality" type="float" min="0" value="10"/>
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107 </section>
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108 <section name="advanced_prot" title="Advanced options for protein coding gene prediction">
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109 <param argument="--evalue" label="BLAST E-value Exponent" help="Negation of the exponent of the E-value threshold used by BLAST, i.e. a value X gives an E-value of 10^(-X)" type="float" value="2" min="1"/>
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110 <param argument="--cutoff" label="Quality cutoff" help="Minimum allowed quality in % of the maximum quality value per reading frame" type="integer" value="50" min="0" max="100"/>
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111 <param argument="--clipfac" label="Clipping factor" help="Clip overlapping proteins with the same name that differ by less than the specified factor" type="float" value="10" min="0"/>
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112 <param argument="--ncbicode" checked="false" label="use start/stop codons as in NCBI (default: learned start/stop codons)" type="boolean" truevalue="--ncbicode" falsevalue=""/>
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113 <param argument="--alarab" checked="false" label="Use the hmmer based method of Al Arab et al. 2016. This will consider the evalue, ncbicode, fragovl, fragfac" type="boolean" truevalue="--alarab" falsevalue=""/>
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114 <param argument="--oldstst" checked="false" label="Use the old start/stop prediction method of MITOS1" type="boolean" truevalue="--oldstst" falsevalue=""/>
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115 </section>
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116 <section name="advanced_ncrna" title="Advanced options for ncRNA gene prediction">
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117 <param argument="--locandgloc" checked="false" label="Run mitfi in glocal and local mode (default: local only)" type="boolean" truevalue="--locandgloc" falsevalue=""/>
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118 <param argument="--ncev" label="e-value to use for inferal fast mode" type="float" min="0" value="0.01"/>
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119 <param argument="--sensitive" checked="false" label="Use infernals sensitive mode only" type="boolean" truevalue="--sensitive" falsevalue=""/>
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120 <param argument="--maxtrnaovl" label="Allow tRNA overlap of up to X nt for mitfi" type="integer" value="50"/>
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121 <param argument="--maxrrnaovl" label="Allow rRNA overlap of up to X nt for mitfi" type="integer" value="50"/>
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122 </section>
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123 </inputs>
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124 <outputs>
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125 <data name="bedout" format="bed" from_work_dir="outdir/result.bed">
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126 <filter>"bed" in str(addoutputs)</filter>
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127 </data>
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128 <data name="mitoout" format="tabular" from_work_dir="outdir/result.mitos" label="${tool.name} on ${on_string}: mito">
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129 <filter>"mito" in str(addoutputs)</filter>
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130 </data>
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131 <data name="gffout" format="gff" from_work_dir="outdir/result.gff" label="${tool.name} on ${on_string}: GFF">
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132 <filter>"gff" in str(addoutputs)</filter>
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133 </data>
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134 <data name="seqout" format="txt" from_work_dir="outdir/result.seq" label="${tool.name} on ${on_string}: TBL">
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135 <filter>"seq" in str(addoutputs)</filter>
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136 </data>
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137 <data name="faa" format="fasta" from_work_dir="outdir/result.faa" label="${tool.name} on ${on_string}: aa FASTA">
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138 <filter>"faa" in str(addoutputs)</filter>
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139 </data>
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140 <data name="fas" format="fasta" from_work_dir="outdir/result.fas" label="${tool.name} on ${on_string}: nt FASTA">
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141 <filter>"fas" in str(addoutputs)</filter>
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142 </data>
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143 <data name="geneorderout" format="fasta" from_work_dir="outdir/result.geneorder" label="${tool.name} on ${on_string}: geneorder">
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144 <filter>"geneorder" in str(addoutputs)</filter>
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145 </data>
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146 <data name="protein_plot_out" format="pdf" from_work_dir="outdir/plots/prot.pdf" label="${tool.name} on ${on_string}: Protein prediction plot">
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147 <filter>"protein_plot" in str(addoutputs)</filter>
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148 </data>
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149 <data name="ncRNA_plot_out" format="pdf" from_work_dir="outdir/plots/rna.pdf" label="${tool.name} on ${on_string}: ncRNA prediction plot">
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150 <filter>"ncRNA_plot" in str(addoutputs)</filter>
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151 </data>
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152 <!--<collection name="ncRNA_structure_plot_ps_out" type="list" label="${tool.name} on ${on_string}: ncRNA postscript structure plots">
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153 <discover_datasets pattern="(?P&lt;name&gt;.+)\.ps" format="ps" directory="outdir/plots" />
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154 <filter>"ncRNA_structure_ps_plots" in str(addoutputs)</filter>
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155 </collection>-->
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156 <collection name="ncRNA_structure_plot_svg_out" type="list" label="${tool.name} on ${on_string}: ncRNA svg structure plots">
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157 <discover_datasets pattern="(?P&lt;name&gt;.+)\.svg" format="svg" directory="outdir/plots" />
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158 <filter>"ncRNA_structure_svg_plots" in str(addoutputs)</filter>
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159 </collection>
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160 <data name="rawout" format="zip" from_work_dir="output.zip" label="${tool.name} on ${on_string}: raw data">
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161 <filter>"raw" in str(addoutputs)</filter>
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162 </data>
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163 </outputs>
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diff changeset
164 <tests>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
165 <!-- default options -->
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
166 <test expect_num_outputs="1">
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
167 <param name="input" value="NC_012920.fasta"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
168 <param name="code" value="2"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
169 <param name="refseqver" value="mitos2-refdata" />
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
170 <output name="bedout" file="mitos2_NC_012920.bed" ftype="bed"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
171 <assert_command>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
172 <has_text text="--code 2"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
173 <has_text text="--finovl 50"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
174 <not_has_text text="--trna"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
175 <not_has_text text="--rrna"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
176 <not_has_text text="--prot"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
177 <has_text text="--intron 0"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
178 <has_text text="--oril 0"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
179 <has_text text="--orih 0"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
180 <has_text text="--evalue 2.0"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
181 <has_text text="--cutoff 0.5"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
182 <has_text text="--clipfac 10.0"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
183 <not_has_text text="--best"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
184 <has_text text="--fragovl 0.2"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
185 <has_text text="--fragfac 10.0"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
186 <has_text text="--ncev 0.01"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
187 <has_text text="--maxtrnaovl 50"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
188 <has_text text="--maxrrnaovl 50"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
189 <has_text text="--noplots"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
190 </assert_command>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
191 </test>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
192 <!-- different main options -->
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
193 <test expect_num_outputs="1">
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
194 <param name="input" value="NC_012920.fasta"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
195 <param name="code" value="5"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
196 <param name="refseqver" value="mitos2-refdata" />
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
197 <output name="bedout" file="mitos2_NC_012920.bed" ftype="bed" compare="sim_size"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
198 <section name="advanced">
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
199 <param name="featuretypes" value="prot,trna,rrna,intron,oril,orih"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
200 <param name="finovl" value="49"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
201 <param name="best" value="true"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
202 <param name="fragovl" value="10"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
203 <param name="fragfac" value="9"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
204 </section>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
205 <assert_command>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
206 <has_text text="--code 5"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
207 <has_text text="--finovl 49"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
208 <not_has_text text="--trna"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
209 <not_has_text text="--rrna"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
210 <not_has_text text="--prot"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
211 <not_has_text text="--intron"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
212 <not_has_text text="--oril"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
213 <not_has_text text="--orih"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
214 <has_text text="--evalue 2.0"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
215 <has_text text="--cutoff 0.5"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
216 <has_text text="--clipfac 10.0"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
217 <has_text text="--best"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
218 <has_text text="--fragovl 0.1"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
219 <has_text text="--fragfac 9.0"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
220 <has_text text="--ncev 0.01"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
221 <has_text text="--maxtrnaovl 50"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
222 <has_text text="--maxrrnaovl 50"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
223 <has_text text="--noplots"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
224 </assert_command>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
225 </test>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
226 <!-- different pcg and ncrn options -->
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
227 <test expect_num_outputs="11">
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
228 <param name="input" value="NC_012920.fasta"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
229 <param name="code" value="2"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
230 <param name="refseqver" value="mitos2-refdata" />
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
231 <section name="advanced_prot">
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
232 <param name="evalue" value="3"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
233 <param name="cutoff" value="49"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
234 <param name="clipfac" value="9"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
235 <param name="ncbicode" value="true"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
236 <param name="alarab" value="true"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
237 <param name="oldstst" value="true"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
238 </section>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
239 <section name="advanced_ncrna">
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
240 <!-- <param name="locandgloc" value="true"/> should be possible from 2.0.5 https://gitlab.com/Bernt/MITOS/-/commit/9b4c55c29961c307dce02ac0319dadbd76f6b9e5-->
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
241 <param name="ncev" value="0.1"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
242 <param name="sensitive" value="true"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
243 <param name="maxtrnaovl" value="51"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
244 <param name="maxrrnaovl" value="49"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
245 </section>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
246 <param name="addoutputs" value="bed,mito,gff,seq,fas,faa,geneorder,protein_plot,ncRNA_plot,ncRNA_structure_svg_plots,raw"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
247 <output name="bedout" file="mitos2_NC_012920.bed" ftype="bed" compare="sim_size"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
248 <output name="mitoout" file="mitos2_NC_012920.mitos" ftype="tabular"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
249 <output name="gffout" file="mitos2_NC_012920.gff" ftype="gff"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
250 <output name="seqout" file="mitos2_NC_012920.seq" ftype="txt"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
251 <output name="faa" file="mitos2_NC_012920.faa" ftype="fasta"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
252 <output name="fas" file="mitos2_NC_012920.fas" ftype="fasta"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
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253 <output name="geneorderout" file="mitos2_NC_012920.geneorder" ftype="fasta"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
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254 <output name="protein_plot_out" file="mitos2_NC_012920_prot.pdf" ftype="pdf" compare="sim_size"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
255 <output name="ncRNA_plot_out" file="mitos2_NC_012920_ncrna.pdf" ftype="pdf" compare="sim_size"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
256 <output name="rawout" ftype="zip">
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
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257 <assert_contents>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
258 <has_archive_member path=".*/result.bed"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
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259 </assert_contents>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
260 </output>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
261 <output_collection name="ncRNA_structure_plot_svg_out" type="list" count="17"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
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262 <assert_command>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
263 <has_text text="--code 2"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
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264 <has_text text="--finovl 50"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
265 <not_has_text text="--trna"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
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266 <not_has_text text="--rrna"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
267 <not_has_text text="--prot"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
268 <has_text text="--intron 0"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
269 <has_text text="--oril 0"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
270 <has_text text="--orih 0"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
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271 <has_text text="--evalue 3.0"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
272 <has_text text="--cutoff 0.49"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
273 <has_text text="--clipfac 9.0"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
274 <has_text text="--alarab"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
275 <has_text text="--oldstst"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
276 <has_text text="--ncbicode"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
277 <not_has_text text="--best"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
278 <has_text text="--fragovl 0.2"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
279 <has_text text="--fragfac 10.0"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
280 <!--<has_text text="\-\-locandgloc"/>-->
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
281 <has_text text="--ncev 0.1"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
282 <has_text text="--sensitive"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
283 <has_text text="--maxtrnaovl 51"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
284 <has_text text="--maxrrnaovl 49"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
285 <not_has_text text="--noplots"/>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
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286 </assert_command>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
287 </test>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
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288 </tests>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
289 <help>@COMMON_HELP@
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
290 <![CDATA[
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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parents:
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291
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
292
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
293 **Advanced options**
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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294
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
295 - Feature types
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
296
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
297 Select the feature types that should be annotated. By default this is protein coding genes, tRNA and rRNA which is useful for metazoan mitogenomes. In addition also the replication origins of the light (OL) and heavy (OH) strand and introns can be annotated. The annotation of the replication origins is most useful for chordate mitogenomes. Introns are usually only found in mitogenomes of non-metazoans and basal Metazoa.
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
298
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
299 - Final overlap (nt)
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
300
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
301 Maximum number of nucleotides by which genes of different types may overlap. Applies to merging of the final predictions.
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
302
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
303 - Annotate only the best copy of each feature
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
304
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
305 If there are copies of the same feature type only the one with the lowest e-value (for ncRNAs and OL) or highest quality score (protein coding genes and OH)
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
306
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
307 - Fragment overlap
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
308
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
309 Maximum fraction (of the shorter feature) allowed that two hits overlap in the query to be counted as fragments.
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
310
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
311 - Fragment quality factor
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
312
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
313 Maximum factor by which fragments may differ in their quality scores. Higher values allow that parts of a gene can differ more in their quality.
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
314
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
315 **Advanced options for protein coding gene prediction**
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
316
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
317 - BLAST E-value Exponent
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
318
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
319 The statistical significance threshold for considering matches in the BLASTX search. The value entered here is the negation of the exponent of the E-value threshold that should be used by BLAST, i.e. a value X gives an E-value of 10^(-X).
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
320
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
321 - Quality cutoff
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
322
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
323 Minimum allowed quality value (in percent) of the maximum quality value per reading frame. A higher values correspond to shorter protein prediction and therefore reduced risk for conflicts with other features
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
324
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
325 - Clipping factor
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
326
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
327 Clipping is started if overlapping prediction of hits with the same name differ by less than a factor X in their quality value.
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
328
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
329 - use start/stop codons as in NCBI (default: learned start/stop codons)
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
330
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
331 Instead of the codon probabilities derived from the protein coding genes annotated in RefSeq the codons listed at NCBI taxonomy are used with equal probabilities (https://www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi)
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
332
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
333 - Use the hmmer based method of Al Arab et al. 2016. This will consider the evalue, ncbicode, fragovl, fragfac parameters
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
334
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
335 Note: 1) this only works for Metazoa RefSeq release 63 reference data set. 2) This will only predict the protein coding genes that are typical for metazoan mitochondrial genomes.
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
diff changeset
336
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
iuc
parents:
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337 - Use the old start/stop prediction method of MITOS1
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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338
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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339 The search for start and stop codons just takes the closest to the initial start / stop positions within 6aa (i.e. the method used in MITOS1)
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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340
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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341 **Advanced options for ncRNA gene prediction**
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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342
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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343 - Run mitfi in glocal and local mode (default: local only)
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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344
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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345 By default mitfi uses infernal's cmsearch in local search mode only. By enabling this option mitfi will invoke cmserach also in glocal mode if a feature is missing.
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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346
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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347 - e-value to use for inferal fast mode
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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348
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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349 The e-value passed to the first pass of cmsearch in the second pass (the sensitive search) an e-value of 0.1 is used.
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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350
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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351 - Use infernal's sensitive mode only
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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352
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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353 By default mitfi searches for ncRNAs using cmsearch's default fast mode first. If a ncRNA type is missing it is searched using the sensitive mode. This can be useful if low scoring copies are expected which might be missed when searching in the two stage mode.
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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354
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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355 - Allow tRNA/rRNA overlap of up to X nt for mitfi
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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356
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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357 Allow that a tRNA/rRNA overlaps with another feature by this number of nucleotides.
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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358
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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359 ]]></help>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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360 <citations>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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361 <citation type="doi">10.1093/nar/gkz833</citation>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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362 <citation type="doi">10.1016/j.ympev.2016.09.024</citation>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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363 </citations>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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364 </tool>
64e557714a07 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mitos commit 791f28c8a7194fdd1ecec05ad166932d461899b2"
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365