Mercurial > repos > iuc > masigpro
annotate masigpro.xml @ 4:21a81335cd13 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
| author | iuc |
|---|---|
| date | Tue, 31 Aug 2021 09:21:26 +0000 |
| parents | a9c93cd4f54b |
| children | 401e7c9e09f2 |
| rev | line source |
|---|---|
|
3
a9c93cd4f54b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit 60951bbe9b08fa6eb2762db9ee15c88e8acf1a1f"
iuc
parents:
2
diff
changeset
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1 <tool id="masigpro" name="maSigPro" version="1.49.3.1+galaxy1"> |
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1
6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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2 <description>Significant Gene Expression Profile Differences in Time Course Gene Expression Data</description> |
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4
21a81335cd13
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
iuc
parents:
3
diff
changeset
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3 <xrefs> |
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21a81335cd13
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
iuc
parents:
3
diff
changeset
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4 <xref type="bio.tools">masigpro</xref> |
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21a81335cd13
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
iuc
parents:
3
diff
changeset
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5 </xrefs> |
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1
6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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6 <requirements> |
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3
a9c93cd4f54b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit 60951bbe9b08fa6eb2762db9ee15c88e8acf1a1f"
iuc
parents:
2
diff
changeset
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7 <requirement type="package" version="8.25">coreutils</requirement> |
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1
6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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8 <requirement type="package" version="1.49.3">bioconductor-masigpro</requirement> |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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9 <requirement type="package" version="1.3.2">r-optparse</requirement> |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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10 <requirement type="package" version="4.4">sed</requirement> |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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11 </requirements> |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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12 <stdio> |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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13 <regex match="Execution halted" |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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14 source="both" |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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15 level="fatal" |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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16 description="Execution halted." /> |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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17 <regex match="Error in" |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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18 source="both" |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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19 level="fatal" |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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20 description="An undefined error occurred, please check your input carefully and contact your administrator." /> |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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21 <regex match="Fatal error" |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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22 source="both" |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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23 level="fatal" |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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24 description="An undefined error occurred, please check your input carefully and contact your administrator." /> |
|
6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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25 </stdio> |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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26 <version_command> |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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27 <![CDATA[ |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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28 echo $(R --version | grep version | grep -v GNU)", maSigPro version" $(R --vanilla --slave -e "library(maSigPro); cat(sessionInfo()\$otherPkgs\$maSigPro\$Version)" 2> /dev/null | grep -v -i "WARNING: ") |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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29 ]]> |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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30 </version_command> |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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31 <command> |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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32 <![CDATA[ |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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33 #if str($source.source_selector) == "advanced": |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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34 paste |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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35 #set $start = True |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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36 #set $header = '' |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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37 #for $time in $source.rep_time: |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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38 #for $file in $time.files: |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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39 #if $start: |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
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changeset
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40 <(cut -f1 $file) |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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41 #set $start = False |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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42 #end if |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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43 #set $header += ' "' + $file.name + '"' |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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44 <(cut -f2 $file) |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
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changeset
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45 #end for |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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46 #end for |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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47 > data && sed -i '1i$header' data && |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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changeset
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48 #if $source.enable_output: |
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3
a9c93cd4f54b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit 60951bbe9b08fa6eb2762db9ee15c88e8acf1a1f"
iuc
parents:
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49 cp $design_matrix $edesign_out && |
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1
6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
0
diff
changeset
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50 #end if |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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changeset
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51 #set $data = 'data' |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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diff
changeset
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52 #set $edesign = $design_matrix |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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diff
changeset
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53 #else: |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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changeset
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54 #set $data = $source.data |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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changeset
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55 #set $edesign = $source.edesign |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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changeset
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56 #end if |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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57 Rscript '${__tool_directory__}/masigpro.R' |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
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58 -e '$edesign' |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
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59 -d '$data' |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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60 -o '$masigpro_out' |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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changeset
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61 #if str($source.source_selector) == "defaults": |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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changeset
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62 --time_col $source.time_col |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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63 --repl_col $source.repl_col |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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64 #end if |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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65 --degree $makeDesignMatrix.degree |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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66 --qvalue $p_vector.qvalue |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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67 --min_obs $p_vector.min_obs |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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68 --step_method '$Tfit.step_method' |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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69 --nvar_correction $Tfit.nvar_correction |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
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70 --alfa $Tfit.alfa |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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71 --rsq $getSiggenes.rsq |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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72 --vars '$getSiggenes.vars' |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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73 --significant_intercept '$getSiggenes.significant_intercept' |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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74 #if $pdf.pdf_selector: |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
parents:
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75 --cluster_data $pdf.seeGenes.clusterData |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
iuc
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76 -k $pdf.seeGenes.k |
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2
0e5ddeb06378
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit 54ebbdebc9861af600371928cb9915095941c231
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77 --print_cluster $pdf.seeGenes.print_cluster |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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78 --cluster_method $pdf.seeGenes.clustering.clusterMethod |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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79 #if str($pdf.seeGenes.clustering.clusterMethod) == "hclust": |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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80 --distance $pdf.seeGenes.clustering.distance |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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81 --agglo_method $pdf.seeGenes.clustering.aggloMethod |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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82 #end if |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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83 #if str($pdf.seeGenes.clustering.clusterMethod) == "kmeans": |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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84 --iter_max $pdf.seeGenes.clustering.iterMax |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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85 #end if |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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86 --color_mode $pdf.seeGenes.colorMode |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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87 --show_fit $pdf.seeGenes.showFit |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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88 --show_lines $pdf.seeGenes.showLines |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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89 --cexlab $pdf.seeGenes.cexlab |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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90 --legend $pdf.seeGenes.legend |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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91 #end if |
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3
a9c93cd4f54b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit 60951bbe9b08fa6eb2762db9ee15c88e8acf1a1f"
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92 #if str($source.source_selector) == "advanced" and $source.enable_output |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit 60951bbe9b08fa6eb2762db9ee15c88e8acf1a1f"
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93 && mv data $data_out |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit 60951bbe9b08fa6eb2762db9ee15c88e8acf1a1f"
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94 #end if |
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1
6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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95 ]]> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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96 </command> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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97 <configfiles> |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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98 <configfile name="design_matrix">#if str($source.source_selector) == "advanced": |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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99 #set $header = "Name Time Replicate" |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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100 #for $group in $source.rep_groups: |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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101 #set $header = $header + ' ' + str($group.name) |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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102 #end for |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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103 $header |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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104 #set $c = len($source.rep_repl) + 1 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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105 #for $time in $source.rep_time: |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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106 #for $file in $time.files: |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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107 #set $is_repl = False |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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108 #for $i, $repl in enumerate($source.rep_repl): |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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109 #if str($file) in str($repl.files): |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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110 #set $r = $i + 1 |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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111 #set $is_repl = True |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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112 #end if |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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113 #end for |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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114 #if $is_repl == False: |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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115 #set $r = $c |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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116 #set $c += 1 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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117 #end if |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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118 #set $line = '"' + str($file.name) + '" ' + str($time.time) + ' ' + str($r) |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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119 #for $group in $source.rep_groups: |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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120 #if str($file) in str($group.files): |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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121 #set $line += " 1" |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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122 #else |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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123 #set $line += " 0" |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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124 #end if |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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125 #end for |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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126 $line |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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127 #end for |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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128 #end for |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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129 #end if |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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130 </configfile> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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131 </configfiles> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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132 <inputs> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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133 <conditional name="source"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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134 <param label="Choose data source" name="source_selector" |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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135 help="Choose if you want to provide seperate count files (e.g. from HTSeq-count or feature-seq) |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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136 and define your experiment design matrix here, or if you have maSigPro edesign and data input files already." |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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137 type="select"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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138 <option value="defaults">Use maSigPro edesign and data files</option> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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139 <option value="advanced">Seperate count data (e.g. from HTSeq-count or feature-count)</option> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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140 </param> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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141 <when value="defaults"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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142 <param name="edesign" format="tabular,txt" type="data" label="Experiment matrix" |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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143 help="Matrix describing experimental design. Rows must be arrays and columns experiment descriptors" /> |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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144 <param name="data" format="tabular,txt" type="data" label="Gene expression matrix" |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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145 help="Matrix containing normalized gene expression data. Genes must be in rows and arrays in columns" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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146 <param name="time_col" label="Column number containing time values" type="integer" value="1" |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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147 help="Column number in edesign containing time values. Default is first column" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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148 <param name="repl_col" label="Column number containing replicate coding" type="integer" value="2" |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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149 help="Column number in edesign containing coding for replicate arrays. Default is second column" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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150 </when> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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151 <when value="advanced"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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152 <param name="enable_output" type="boolean" truevalue="1" falsevalue="0" checked="false" label="Output generated maSigPro input files?" |
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153 help="Choose if you want to output the generated edesign and data files for direct use in maSigPro as history elements." /> |
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154 <repeat name="rep_time" title="Time values" min="1" default="1"> |
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155 <param name="time" type="integer" value="0" label="Specify a numerical time value" help="Only numbers will be allowed"> |
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156 <sanitizer> |
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157 <valid initial="string.digits"></valid> |
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158 </sanitizer> |
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159 </param> |
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160 <param name="files" type="data" format="tabular" multiple="true" label="Counts file(s) at this time value" /> |
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161 </repeat> |
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162 <repeat name="rep_groups" title="Experimental groups" min="1" default="1"> |
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163 <param name="name" type="text" value="Group title" label="Specify the name of this experimental group" |
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164 help="Use a single name without spaces or special characters"> |
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165 </param> |
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166 <param name="files" type="data" format="tabular" multiple="true" |
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167 label="Counts file(s) belonging to this experimental group" /> |
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168 </repeat> |
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169 <repeat name="rep_repl" title="Replicates" min="0" default="0"> |
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170 <param name="files" type="data" format="tabular" multiple="true" label="Counts files that are replicates" /> |
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171 </repeat> |
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172 </when> |
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173 </conditional> |
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174 <section name="makeDesignMatrix" |
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175 title="Step 1: make.Design.Matrix - Defining the regression model" |
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176 help="‘make.design.matrix’ creates the design matrix of dummies for |
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177 fitting time series micorarray gene expression experiments."> |
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178 <param name="degree" type="integer" value="1" |
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179 label="Degree of regression fit polynome" |
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180 help="The degree of the regression fit polynome. ‘degree’ = 1 returns |
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181 linear regression, ‘degree’ = 2 returns quadratic regression, etc" /> |
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182 </section> |
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183 <section name="p_vector" |
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184 title="Step 2: p.vector - Finding significant genes" |
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185 help="‘p.vector’ performs a regression fit for each gene taking all |
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186 variables present in the model given by a regression matrix and |
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187 returns a list of FDR corrected significant genes"> |
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188 <param name="qvalue" type="float" value="0.05" label="Q" help="Significance level" /> |
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189 <param name="min_obs" label="Minimum values" type="integer" value="6" |
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190 help="Genes with less than this number of true numerical values |
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191 will be excluded from the analysis. Minimum value to estimate |
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192 the model is (degree+1)xGroups+1. Default is 6." /> |
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193 </section> |
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194 <section name="Tfit" title="Step 3: T.fit - Finding significant differences" |
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195 help="‘T.fit’ selects the best regression model for each gene using |
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196 stepwise regression. In the maSigPro approach ‘p.vector’ and ‘T.fit’ are subsequent |
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197 steps, meaning that significant genes are first selected on the |
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198 basis of a general model and then the significant variables for |
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199 each gene are found by step-wise regression."> |
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200 <param name="step_method" type="select" label="Step regression" |
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201 help="The step regression can be ‘backward’ or ‘forward’ indicating |
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202 whether the step procedure starts from the model with all or none |
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203 variables. With the ‘two.ways.backward’ or ‘two.ways.forward’ |
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204 options the variables are both allowed to get in and out. At each |
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205 step the p-value of each variable is computed and variables get |
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206 in/out the model when this p-value is lower or higher than given |
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207 threshold alfa."> |
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208 <option selected="True" value="backward">backward</option> |
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209 <option value="forward">forward</option> |
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210 <option value="two.ways.backward">two.ways.backward</option> |
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211 <option value="two.ways.forward">two.ways.forward</option> |
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212 </param> |
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213 <param type="boolean" name="nvar_correction" label="nvar correction" truevalue="TRUE" falsevalue="FALSE" checked="false" |
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214 help="When nvar.correction is TRUE the given significance |
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215 level is corrected by the number of variables in the model."> |
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216 <option selected="True" value="FALSE">False</option> |
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217 <option value="TRUE">True</option> |
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218 </param> |
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219 <param name="alfa" type="float" value="0.05" label="alfa" help="Significance level used for variable selection in the stepwise regression" /> |
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220 </section> |
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221 <section name="getSiggenes" |
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222 title="Step 4: get.siggenes - Obtaining lists of significant genes" |
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223 help="This function creates lists of significant genes for a set of |
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224 variables whose significance value has been computed with the |
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225 ‘T.fit’ function."> |
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226 <param name="rsq" type="float" value="0.7" label="rsq" |
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227 help="cut-off level at the R-squared value for the stepwise |
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228 regression fit. Only genes with R-squared more than rsq are |
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229 selected" /> |
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230 <param name="vars" type="select" label="Variables" |
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231 help="Variables for which to extract significant genes. |
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232 ‘all’: generates one single matrix or gene list with all |
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233 significant genes. |
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234 |
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235 ‘each’: generates as many significant genes extractions as |
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236 variables in the general regression model. Each extraction |
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237 contains the significant genes for that variable. |
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238 |
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239 ‘groups’: generates a significant genes extraction for each |
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240 experimental group. |
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241 |
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242 The difference between ‘each’ and ‘groups’ is that in the |
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243 first case the variables of the same group (e.g. ‘TreatmentA’ |
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244 and ‘time*TreatmentA’) will be extracted separately and in t |
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245 he |
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246 second case jointly."> |
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247 <option selected="True" value="groups">Groups</option> |
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248 <option value="each">Each</option> |
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249 <option value="all">All</option> |
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250 </param> |
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251 <param name="significant_intercept" type="select" label="Significant intercept" |
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252 help="The argument ‘significant.intercept’ modulates the treatment for |
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253 intercept coefficients to apply for selecting significant genes |
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254 when ‘vars’ equals ‘groups’. There are three possible values: |
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255 ‘none’, no significant intercept (differences) are considered |
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256 for significant gene selection, ‘dummy’, includes genes with |
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257 significant intercept differences between control and experimental |
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258 groups, and ‘all’ when both significant intercept coefficient |
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259 for the control group and significant intercept differences are |
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260 considered for selecting significant genes."> |
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261 <option selected="True" value="dummy">Dummy</option> |
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262 <option value="none">None</option> |
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263 <option value="all">All</option> |
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264 </param> |
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265 </section> |
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266 <conditional name="pdf"> |
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267 <param label="Generate visualization PDF" name="pdf_selector" type="boolean" |
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268 truevalue="1" falsevalue="0" checked="true" |
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269 help="Choose if you want to generate a PDF file containing the visualizations" /> |
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270 <when value="1"> |
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271 <section name="seeGenes" title="Step 5: see.genes - Visualization" |
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272 help="This function provides visualisation tools for gene expression |
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273 values in a time course experiment. The function first calls the |
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274 heatmap function for a general overview of experiment results. |
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275 Next a partioning of the data is generated using a clustering |
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276 method. The results of the clustering are visualized both as gene |
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277 expression profiles extended along all arrays in the experiment, |
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278 as provided by the plot.profiles function, and as summary |
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279 expression profiles for comparison among experimental groups."> |
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280 <param name="clusterData" label="Cluster Data" type="integer" value="1" |
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281 help="Data clustering can be done on the basis of either the original |
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282 expression values, the regression coefficients, or the t.scores. |
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283 In case ‘data’ is a ‘get.siggenes’ object, this is given by |
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284 providing the element names of the list |
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285 ‘c(sig.profiles,coefficients,t.score)’ of their list |
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286 position (1,2 or 3)." /> |
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287 <param name="k" type="integer" label="Number of clusters for data partioning" value="9" /> |
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288 <param name="print_cluster" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="true" |
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289 label="Add cluster information to summary file?" |
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290 help="Adds columns with the cluster assignment for each gene." /> |
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291 <conditional name="clustering"> |
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292 <param name="clusterMethod" label="Cluster Method" type="select" |
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293 help="clustering method for data partioning. Currently |
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294 ‘hclust’, ‘kmeans’ and ‘Mclust’ are supported"> |
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295 <option selected="True" value="hclust">hclust</option> |
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296 <option value="kmeans">kmeans</option> |
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297 <option value="Mclust">Mclust</option> |
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298 </param> |
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299 <when value="hclust"> |
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300 <param name="distance" type="select" label="Distance measure" |
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301 help="Distance measurement function when ‘cluster.method’ is |
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302 ‘hclust’. Default uses correlation."> |
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303 <option selected="True" value="cor">Correlation</option> |
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304 <option value="euclidean">Euclidean</option> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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305 <option value="maximum">Maximum</option> |
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306 <option value="manhattan">Manhattan</option> |
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307 <option value="Canberra">Canberra</option> |
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308 <option value="binary">Binary</option> |
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309 <option value="minkowski">Minkowski</option> |
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310 </param> |
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311 <param name="aggloMethod" type="select" label="Agglomeration method" |
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312 help="The agglomeration method to be used when ‘cluster.method’ is ‘hclust’."> |
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313 <option selected="True" value="ward.D">ward.D</option> |
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314 <option value="ward.D2">ward.D2</option> |
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315 <option value="single">single</option> |
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316 <option value="complete">complete</option> |
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317 <option value="average">average (= UPGMA)</option> |
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318 <option value="mcquitty">mcquitty (= WPGMA)</option> |
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319 <option value="median">median (= WPGMC)</option> |
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320 <option value="centroid">centroid (= UPGMC)</option> |
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321 </param> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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322 </when> |
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323 <when value="kmeans"> |
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324 <param name="iterMax" type="integer" label="Maximum number of iterations" value="500" |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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325 help="Maximum number of iterations when ‘cluster.method’ is ‘kmeans’" /> |
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326 </when> |
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2
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327 <when value="Mclust"> |
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328 </when> |
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1
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329 </conditional> |
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330 <param name="colorMode" label="Color Mode" type="select" help="Color scale for plotting profiles. Can be either ‘rainbow’ or ‘gray’"> |
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331 <option selected="True" value="rainbow">Rainbow</option> |
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332 <option value="gray">Gray</option> |
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333 </param> |
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334 <param name="showFit" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="true" label="Show regression fit curves?" |
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335 help="Indicating whether regression fit curves must be plotted" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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336 <param name="showLines" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="true" label="Draw lines?" |
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337 help="Indicating whether a line must be drawn joining plotted data points for each group" /> |
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338 <param name="cexlab" type="float" value="0.8" label="Magnification for x labels" |
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339 help="Graphical parameter maginfication to be used for x labels in plotting functions" /> |
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340 <param name="legend" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="true" label="Add legend to plotting profiles?" |
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6bc58c43b997
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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341 help="Indicating whether legend must be added when plotting profiles" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/masigpro commit c4510bf402d10e8e3a3c4c90c2d96666c987a256
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342 </section> |
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343 </when> |
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2
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344 <when value="0"> |
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345 </when> |
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1
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346 </conditional> |
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347 </inputs> |
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348 <outputs> |
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349 <data format="txt" name="data_out" label="maSigPro data file on ${on_string}"> |
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350 <filter> |
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351 (( |
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352 source['source_selector'] == 'advanced' and |
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353 source['enable_output'] == True |
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354 )) |
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355 </filter> |
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1
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356 </data> |
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357 <data format="txt" name="edesign_out" label="maSigPro edesign file on ${on_string}"> |
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358 <filter> |
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359 (( |
|
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360 source['source_selector'] == 'advanced' and |
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361 source['enable_output'] == True |
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362 )) |
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363 </filter> |
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364 </data> |
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365 <data format="pdf" name="pdf_out" from_work_dir="Results.pdf" label="maSigPro Plot file on ${on_string}"> |
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366 <filter> |
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367 (( |
|
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368 pdf['pdf_selector'] == True |
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369 )) |
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370 </filter> |
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371 </data> |
|
2
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372 <data format="tabular" name="masigpro_out" label="maSigPro result file on ${on_string}"> |
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373 </data> |
|
1
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374 </outputs> |
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375 <tests> |
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376 <test> |
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377 <param name="source_selector" value="advanced" /> |
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378 <param name="enable_output" value="1" /> |
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379 <repeat name="rep_time"> |
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380 <param name="time" value="1" /> |
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381 <param name="files" value="control_1H.counts,treat_1H.counts" /> |
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382 </repeat> |
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383 <repeat name="rep_time"> |
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384 <param name="time" value="2" /> |
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385 <param name="files" value="control_2H.counts,treat_2H.counts" /> |
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386 </repeat> |
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387 <repeat name="rep_time"> |
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388 <param name="time" value="3" /> |
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389 <param name="files" value="control_3H.counts,treat_3H_1.counts,treat_3H_2.counts" /> |
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390 </repeat> |
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391 <param name="replicates_selector" value="advanced" /> |
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392 <repeat name="rep_repl"> |
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393 <param name="files" value="treat_3H_1.counts,treat_3H_2.counts" /> |
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394 </repeat> |
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395 <repeat name="rep_groups"> |
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396 <param name="name" value="Control" /> |
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397 <param name="files" value="control_1H.counts,control_2H.counts,control_3H.counts" /> |
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398 </repeat> |
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399 <repeat name="rep_groups"> |
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400 <param name="name" value="Treatment" /> |
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401 <param name="files" value="treat_1H.counts,treat_2H.counts,treat_3H_1.counts,treat_3H_2.counts" /> |
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402 </repeat> |
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403 <output name="masigpro_out" file="masigpro_out.tab" /> |
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404 <output name="data_out" file="data_out.txt" /> |
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405 <output name="edesign_out" file="edesign_out.txt" /> |
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406 <output name="pdf_out" file="Results.pdf" /> |
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407 </test> |
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408 <test> |
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409 <param name="source_selector" value="defaults" /> |
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410 <param name="edesign" value="edesign_out.txt" /> |
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411 <param name="data" value="data_out.txt" /> |
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412 <output name="masigpro_out" file="masigpro_out.tab" /> |
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413 <output name="pdf_out" file="Results.pdf" /> |
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414 </test> |
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2
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415 <test> |
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416 <param name="source_selector" value="defaults" /> |
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417 <param name="edesign" value="edesign_out.txt" /> |
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418 <param name="data" value="data_out.txt" /> |
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419 <param name="print_cluster" value="FALSE" /> |
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420 <output name="masigpro_out" file="masigpro_out_no_cluster.tab" /> |
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421 <output name="pdf_out" file="Results.pdf" /> |
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422 </test> |
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1
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423 </tests> |
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424 <help> |
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425 <![CDATA[ |
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426 .. class:: infomark |
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427 |
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428 **What it does** |
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429 |
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430 maSigPro_ is a regression based approach to find genes for which there are significant gene expression profile differences between experimental groups in time course microarray and RNA-Seq experiments. |
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431 |
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432 **Inputs** |
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433 |
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434 The maSigPro wrapper has two options for input data: |
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435 |
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436 - directly through two seperate text files containing the experiment design (edesign) and the data or |
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437 - count tables generated from HTSeq-count. Count tables must be generated for each sample individually. |
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438 |
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439 To set up an experimental design from seperate count files you first have to select which files belong to a certain time point. |
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440 Likewise you can specify which files are replicates. In a third step you have to create the experimental groups and select the related files. |
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441 For a more comfortable setup in future analysis you have the option to output the generated edesign and data files. |
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442 |
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443 **Output** |
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444 |
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445 maSigPro_ generates a summary file containing the list of significant genes. Additionally you can obtain a PDF file containing plots of profiles and groups that visualize the clustering analysis. |
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446 |
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447 .. _maSigPro: https://bioconductor.org/packages/release/bioc/html/maSigPro.html |
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448 ]]> |
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449 </help> |
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450 <citations> |
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451 <citation type="doi">10.1093/bioinformatics/btl056</citation> |
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452 </citations> |
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453 </tool> |
