Mercurial > repos > iuc > isoformswitchanalyzer

annotate isoformswitchanalyzer.xml @ 0:bb611fa3bc3b draft

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
author iuc
date Tue, 24 Jan 2023 18:36:55 +0000
parents
children 5ae218cee629
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0
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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1 <tool id="isoformswitchanalyzer" name="IsoformSwitchAnalyzeR" version="@TOOL_VERSION@+galaxy@SUFFIX_VERSION@">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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2 <description>statistical identification of isoform switching</description>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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3 <macros>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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4 <import>macros.xml</import>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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5 </macros>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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6 <expand macro='xrefs'/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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7 <expand macro='requirements'/>
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8 <stdio>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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9 <regex match="Execution halted"
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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10 source="both"
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11 level="fatal"
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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12 description="Execution halted." />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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13 <regex match="Error in"
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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14 source="both"
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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15 level="fatal"
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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16 description="An undefined error occurred, please check your input carefully and contact your administrator." />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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17 <regex match="Fatal error"
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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18 source="both"
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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19 level="fatal"
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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20 description="An undefined error occurred, please check your input carefully and contact your administrator." />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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21 </stdio>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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22 <command><![CDATA[
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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23 #if $functionMode.selector == 'data_import'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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24 #if $functionMode.transcriptome.is_of_type("fasta.gz"):
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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25 ln -s '${functionMode.transcriptome}' './transcriptome.fasta.gz' &&
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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26 #set $transcriptome = './transcriptome.fasta.gz'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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27 #else
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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28 ln -s '${functionMode.transcriptome}' './transcriptome.fasta'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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29 #set $transcriptome = './transcriptome.fasta'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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30 #end if
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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31 #if $functionMode.genomeAnnotation.is_of_type("gtf.gz"):
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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32 ln -s '${functionMode.genomeAnnotation}' './annotation.gtf.gz' &&
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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33 #set $annotation = './annotation.gtf.gz'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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34 #else
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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35 ln -s '${functionMode.genomeAnnotation}' './annotation.gtf' &&
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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36 #set $annotation = './annotation.gtf'
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37 #end if
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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38 mkdir -p 'input_files' &&
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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39 #if $functionMode.countFiles != 'disabled':
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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40 mkdir -p "count_files/factor1/" &&
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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41 mkdir -p "count_files/factor2/" &&
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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42 #end if
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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43 #if $functionMode.tool_source.selector == 'stringtie'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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44 #set $filename = 't_data.ctab'
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45 #else
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46 #set $filename = 'quant.sf'
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47 #end if
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48
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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49 #for $index in range(len($functionMode.first_factor.trans_counts)):
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50 mkdir './input_files/${functionMode.first_factor.factorLevel}${index}/' &&
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51 ln -s $functionMode.first_factor.trans_counts[$index] './input_files/${functionMode.first_factor.factorLevel}${index}/${filename}' &&
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52 #end for
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53
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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54 #for $index in range(len($functionMode.second_factor.trans_counts)):
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55 mkdir './input_files/${functionMode.second_factor.factorLevel}${index}/' &&
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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56 ln -s $functionMode.second_factor.trans_counts[$index] './input_files/${functionMode.second_factor.factorLevel}${index}/${filename}' &&
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57 #end for
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58
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59 Rscript '${__tool_directory__}/IsoformSwitchAnalyzeR.R'
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60 --modeSelector $functionMode.selector
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61 --parentDir './input_files'
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62 --annotation $annotation
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63 --transcriptome $transcriptome
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64 --toolSource $functionMode.tool_source.selector
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65 #if $functionMode.tool_source.selector == 'stringtie'
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66 --readLength $functionMode.tool_source.averageSize
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67 $functionMode.tool_source.fixStringTieAnnotationProblem
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68 #end if
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69 --countFiles $functionMode.countFiles
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70 #else if $functionMode.selector == 'first_step'
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71 Rscript '${__tool_directory__}/IsoformSwitchAnalyzeR.R'
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72 --modeSelector $functionMode.selector
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73 --rObject $functionMode.robject
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74 --alpha $functionMode.alpha
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75 --dIFcutoff $functionMode.dIFcutoff
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76 $functionMode.onlySigIsoforms
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77 $functionMode.filterForConsequences
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78 --geneExpressionCutoff $functionMode.prefilter.geneExpressionCutoff
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79 --isoformExpressionCutoff $functionMode.prefilter.isoformExpressionCutoff
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80 --IFcutoff $functionMode.prefilter.IFcutoff
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81 $functionMode.prefilter.removeSingleIsformGenes
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82 $functionMode.prefilter.keepIsoformInAllConditions
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83 $functionMode.dexseq.correctForConfoundingFactors
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84 $functionMode.dexseq.overwriteIFvalues
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85 $functionMode.dexseq.reduceToSwitchingGenes
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86 $functionMode.dexseq.reduceFurtherToGenesWithConsequencePotential
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87 $functionMode.dexseq.keepIsoformInAllConditions
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
88 --minORFlength $functionMode.novel_isoform.minORFlength
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
89 --orfMethod $functionMode.novel_isoform.orfMethod
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
90 --PTCDistance $functionMode.novel_isoform.PTCDistance
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
91 $functionMode.extract_sequence.removeShortAAseq
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
92 $functionMode.extract_sequence.removeLongAAseq
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
93 $functionMode.extract_sequence.removeORFwithStop
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
94 $functionMode.extract_sequence.onlySwitchingGenes
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
95 #else
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
96 #if $functionMode.protein_domains.selector == 'enabled'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
97 mkdir -p './pfam_files' &&
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
98 #for $index,$filename in enumerate($functionMode.protein_domains.analyzePFAM)
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
99 ln -s $filename './pfam_files/dataset${index}.txt' &&
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
100 #end for
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
101 #end if
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
102 #if $functionMode.signal_peptides.selector == 'enabled'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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103 mkdir -p './signalp_files' &&
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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104 #for $index,$filename in enumerate($functionMode.signal_peptides.analyzeSignalP)
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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105 ln -s $filename './signalp_files/dataset${index}.txt' &&
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
106 #end for
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
107 #end if
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
108 #if $functionMode.disordered_regions.selector == 'netsurfp'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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109 mkdir -p './netsurf_files' &&
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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110 #for $index,$filename in enumerate($functionMode.disordered_regions.analyzeNetSurfP2)
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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111 ln -s $filename './netsurf_files/dataset${index}.txt' &&
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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112 #end for
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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113 #end if
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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114 Rscript '${__tool_directory__}/IsoformSwitchAnalyzeR.R'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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115 --modeSelector $functionMode.selector
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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116 --rObject $functionMode.robject
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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117 --analysisMode $functionMode.analysis_mode.selector
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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118 --alpha $functionMode.analysis_mode.alpha
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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119 --dIFcutoff $functionMode.analysis_mode.dIFcutoff
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
120 #if $functionMode.analysis_mode.selector == 'top'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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121 --genesToPlot $functionMode.analysis_mode.n
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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122 $functionMode.analysis_mode.advanced_options.filterForConsequences
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
123 $functionMode.analysis_mode.advanced_options.sortByQvals
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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124 $functionMode.analysis_mode.advanced_options.onlySigIsoforms
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
125 $functionMode.analysis_mode.advanced_options.onlySwitchingGenes
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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126 $functionMode.analysis_mode.advanced_options.countGenes
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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127 $functionMode.analysis_mode.advanced_options.asFractionTotal
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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128 $functionMode.analysis_mode.advanced_options.plotGenes
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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129 $functionMode.analysis_mode.advanced_options.simplifyLocation
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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130 $functionMode.analysis_mode.advanced_options.removeEmptyConsequences
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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131 $functionMode.analysis_mode.advanced_options.removeEmptyConsequences
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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132 #else
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133 --gene $functionMode.analysis_mode.gene
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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134 --IFcutoff $functionMode.analysis_mode.advanced_options.IFcutoff
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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135 $functionMode.analysis_mode.advanced_options.rescaleTranscripts
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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136 $functionMode.analysis_mode.advanced_options.reverseMinus
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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137 $functionMode.analysis_mode.advanced_options.addErrorbars
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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138 $functionMode.analysis_mode.advanced_options.onlySwitchingGenes
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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139 #end if
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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140 #if $functionMode.coding_potential.selector == 'cpat'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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141 --pathToCPATresultFile $functionMode.coding_potential.analyzeCPAT
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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142 --codingCutoff $functionMode.coding_potential.codingCutoff
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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143 #else if $functionMode.coding_potential.selector == 'cpc2'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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144 --pathToCPC2resultFile $functionMode.coding_potential.analyzeCPC2
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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145 $functionMode.coding_potential.removeNoncodingORFs
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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146 --codingCutoff $functionMode.coding_potential.codingCutoff
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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147 #end if
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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148 #if $functionMode.protein_domains.selector == 'enabled'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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149 --pathToPFAMresultFile './pfam_files'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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150 #end if
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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151 #if $functionMode.signal_peptides.selector == 'enabled'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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152 --pathToSignalPresultFile './signalp_files'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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153 --minSignalPeptideProbability $functionMode.signal_peptides.minSignalPeptideProbability
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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154 #end if
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155 #if $functionMode.disordered_regions.selector == 'netsurfp'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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156 --pathToNetSurfP2resultFile './netsurf_files'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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157 --smoothingWindowSize $functionMode.disordered_regions.smoothingWindowSize
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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158 --probabilityCutoff $functionMode.disordered_regions.probabilityCutoff
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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159 --minIdrSize $functionMode.disordered_regions.minIdrSize
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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160 #else if $functionMode.disordered_regions.selector == 'iupred2a'
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
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161 --pathToIUPred2AresultFile $functionMode.disordered_regions.AanalyzeIUPred2A
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
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162 --smoothingWindowSize $functionMode.disordered_regions.smoothingWindowSize
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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163 --probabilityCutoff $functionMode.disordered_regions.probabilityCutoff
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
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164 --minIdrSize $functionMode.disordered_regions.minIdrSize
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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165 $functionMode.disordered_regions.annotateBindingSites
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
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166 --minIdrBindingSize $functionMode.disordered_regions.minIdrBindingSize
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
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167 --minIdrBindingOverlapFrac $functionMode.disordered_regions.minIdrBindingOverlapFrac
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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168 #end if
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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169 --ntCutoff $functionMode.analyzeSwitchConsequences.ntCutoff
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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170 #if $functionMode.analyzeSwitchConsequences.ntFracCutoff
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
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171 --ntFracCutoff $functionMode.analyzeSwitchConsequences.ntFracCutoff
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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172 #end if
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
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173 --ntJCsimCutoff $functionMode.analyzeSwitchConsequences.ntJCsimCutoff
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174 --AaCutoff $functionMode.analyzeSwitchConsequences.AaCutoff
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175 --AaFracCutoff $functionMode.analyzeSwitchConsequences.AaFracCutoff
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176 --AaJCsimCutoff $functionMode.analyzeSwitchConsequences.AaJCsimCutoff
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177 $functionMode.analyzeSwitchConsequences.removeNonConseqSwitches
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178 #if $functionMode.analysis_mode.selector == 'top'
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179 && mkdir -p './pdf_outputs/'
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180 && mv *pdf './pdf_outputs/'
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181 && mv *_vs_* gene_plots
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182 #end if
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183 #end if
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184 ]]></command>
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185 <inputs>
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186 <conditional name="functionMode">
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187 <param name="selector" type="select" label="Tool function mode"
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188 help="The first step of a IsoformSwitchAnalyzeR workflow is to import and integrate the isoform quantification
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189 with its basic annotation. Once you have all the relevant data imported into R (IsoformSwitchAnalyzeR will
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190 also help you with that), the workflow for identification and analysis of isoform switches with functional
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191 consequences can be divided into two parts.">
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192 <option value="data_import">Import data</option>
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193 <option value="first_step">Analysis part one: Extract isoform switches and their sequences</option>
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194 <option value="second_step">Analysis part two: Plot all isoform switches and their annotation</option>
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195 </param>
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196 <when value="data_import">
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197 <section name="first_factor" title="1: Factor level" expanded="true">
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198 <param name="factorLevel" type="text" value="FactorLevel" label="Specify a factor level, typical values could be 'tumor' or 'treated'"
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199 help="Only letters, numbers and underscores will be retained in this field">
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200 <sanitizer>
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201 <valid initial="string.letters,string.digits"><add value="_" /></valid>
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202 </sanitizer>
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203 </param>
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204 <param name="trans_counts" type="data" format="tabular" multiple="true" label="Transcript-level expression measurements"/>
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205 </section>
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206 <section name="second_factor" title="2: Factor level" expanded="true">
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207 <param name="factorLevel" type="text" value="FactorLevel" label="Specify a factor level, typical values could be 'tumor' or 'treated'"
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208 help="Only letters, numbers and underscores will be retained in this field">
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209 <sanitizer>
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210 <valid initial="string.letters,string.digits"><add value="_" /></valid>
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211 </sanitizer>
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212 </param>
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213 <param name="trans_counts" type="data" format="tabular" multiple="true" label="Transcript-level expression measurements"/>
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214 </section>
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215 <conditional name="tool_source">
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216 <param name="selector" type="select" label="Quantification data source" help="IsoformSwitchAnalyzeR has different functions for importing data from different sources.">
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217 <option value="stringtie">StringTie</option>
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218 <option value="salmon">Salmon/Kallisto</option>
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219 </param>
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220 <when value="salmon"/>
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221 <when value="stringtie">
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222 <param name="averageSize" type="integer" min="0" value="150" label="Average read length" help="Must be the number of base pairs sequenced. e.g. if the data
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223 quantified is 75 bp paired ends the the user should supply readLength=75" />
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224 <param argument="fixStringTieAnnotationProblem" type="boolean" truevalue="--fixStringTieAnnotationProblem" falsevalue="" checked="true"
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225 label="Fix StringTie annotation problem" help="This option will automatically try and correct some of the annoation problems created when
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226 doing transcript assembly (unassigned transcripts and merged genes)" />
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227 </when>
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228 </conditional>
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229 <param name="genomeAnnotation" type="data" format="gtf,gtf.gz" label="Genome annotation (GTF)"
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230 help="It is used to integrate the coding sequence (CDS) regions from in the GTF file as the ORF regions used by IsoformSwitchAnalyzeR." />
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231 <param name="transcriptome" type="data" format="fasta,fasta.gz" label="Transcriptome"
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232 help="Please note this different from a fasta file with the sequences of the entire genome." />
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233 <param name="countFiles" type="select" label="Generate count matrix files" help="If IsoformSwitchAnalyzeR is used for fixing Stringtie annotation
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234 problem, it can generate count files for analyzing differential expression with DESeq2 (when selecting collection) or CEMiTool (when secting the expression matrix format).">
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235 <option value="disabled">Disabled</option>
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236 <option value="collection">Collection of count files</option>
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237 <option value="matrix">Expression matrix</option>
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238 </param>
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239 </when>
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240
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241 <!--WRAPPER FIRST STEP SECTION-->
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242
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243 <when value="first_step">
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244 <param name="robject" type="data" format="rdata" label="IsoformSwitchAnalyzeR R object" help="It is generated when running the analysis part 1." />
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245 <expand macro="macro_alpha_difcutoff"/>
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246 <expand macro="macro_onlysigisoforms1"/>
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247 <param argument="filterForConsequences" type="boolean" truevalue="--filterForConsequences" falsevalue="" checked="false"
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248 label="Filter for consquences" help="Filter for genes with functional consequences. The output will then be the number of significant genes
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249 and isoforms originating from genes with predicted consequences" />
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250 <section name="prefilter" title="Pre-filter parameters" help="SwitchAnalyzeR will remove genes/isoforms with the aim of allowing faster
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251 processing time as well as more trustworthy results.">
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252 <param argument="geneExpressionCutoff" type="float" min="0" value="1" label="Gene expression cutoff" help="The expression cutoff (most
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253 likely in TPM/RPKM/FPKM) which the average expression in BOTH condisions must be higher than." />
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254 <param argument="isoformExpressionCutoff" type="float" min="0" value="0" label="Isoform expresion cutoff" help="The expression cutoff (most
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255 likely in RPKM/FPKM) which isoforms must be expressed more than, in at least one conditions of a comparison. Default is 0 (which removes
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256 completely unused isoforms)." />
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257 <expand macro="macro_ifcutoff" value="0.01" help="The cutoff on isoform usage (measured as Isoform Fraction) which isoforms must be used more
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258 than in at least one conditions of a comparison" />
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259 <param argument="removeSingleIsformGenes" type="boolean" truevalue="--removeSingleIsformGenes" falsevalue="" checked="true" label="Remove single isoform genes"
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260 help="Only keep genes containing more than one isoform (in any comparison, after the other filters have been applied)" />
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261 <expand macro="macro_keeisoforminall" checked="false"/>
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262 </section>
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263 <section name="dexseq" title="DEXseq parameters" help="DEXSeq is used to test isoforms (isoform resolution) for differential isoform usage.">
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264 <param argument="correctForConfoundingFactors" type="boolean" truevalue="--correctForConfoundingFactors" falsevalue="" checked="true" label="Correct for confounding factors"
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265 help="A logic indicating whether IsoformSwitchAnalyzeR to use limma to correct for any confounding effects (e.g. batch effects) as indicated in the
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266 design matrix (as additional columns (apart from the two default columns)) " />
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267 <param argument="overwriteIFvalues" type="boolean" truevalue="--overwriteIFvalues" falsevalue="" checked="true" label="Overwrite IF values" help="It indicates
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268 whether to overwrite the IF and dIF stored in the switchAnalyzeRlist with the corrected IF and dIF values - if no confounding effects are
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269 present in the design matrix this will not change anything" />
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270 <param argument="reduceToSwitchingGenes" type="boolean" truevalue="--reduceToSwitchingGenes" falsevalue="" checked="true" label="Reduce to switch genes" help="Reduced to the
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271 genes which contains at least one isoform significantly differential used (as indicated by the alpha and dIFcutoff parameters" />
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272 <param argument="reduceFurtherToGenesWithConsequencePotential" type="boolean" truevalue="--reduceFurtherToGenesWithConsequencePotential" falsevalue="" checked="false"
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273 label="Reduce to genes with consequence potential" help="This argument is a more strict version of reduceToSwitchingGenes
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274 as it not only requires that at least one isoform is significantly differential used (as indicated by the alpha and dIFcutoff parameters) but also that there is an isoform
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275 with the opposite effect size (e.g. used less if the first isoform is used more). The minimum effect size of the opposing isoform usage is also controlled by dIFcutoff.
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276 The existence of such an opposing isoform means a switch pair can be formed" />
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277 <expand macro="macro_keeisoforminall" checked="true"/>
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278 </section>
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279 <section name="novel_isoform" title="Novel isoform analysis parameters" help="For the subset of isoforms not already annotated with ORFs this
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280 function predicts the most likely Open Reading Frame (ORF) and the NMD sensitivity. This function is made to help annotate
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281 isoforms if you have performed (guided) de-novo isoform reconstruction (isoform deconvolution).">
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282 <param argument="minORFlength" type="integer" min="0" value="100" label="Minimum ORF length" help="The minimum size (in nucleotides) an
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283 ORF must be to be considered (and reported). Default is 100 nucleotides, which around 97.5% of Gencode coding isoforms in both human and mouse have." />
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284 <param argument="orfMethod" type="select" label="ORF identification method" help="More information in the help section">
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285 <option value="longest.AnnotatedWhenPossible">Longest and annotated when possible</option>
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286 <option value="longest">Longest</option>
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287 <option value="mostUpstream">Most upstream</option>
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288 <option value="longestAnnotated">Longest annotated</option>
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289 <option value="mostUpstreamAnnoated">Most upstream annotated</option>
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290 </param>
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291 <param argument="PTCDistance" type="integer" min="0" value="50" label="Maximal allowed premature termination codon-distance" help="The minimum
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292 distance (number of nucleotides) from the STOP codon to the final exon-exon junction. If the distance from the STOP to the final exon-exon
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293 junction is larger than this the isoform to be marked as NMD-sensitive. " />
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294 </section>
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295 <section name="extract_sequence" title="Sequence extraction parameters" help="switchAnalyzeR will extracts the nucleotide (NT) sequence of transcripts by
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296 extracting and concatenating the sequences of a reference genome corresponding to the genomic coordinates of the isoforms. ">
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297 <expand macro="macro_onlyswitching"/>
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298 <param argument="removeShortAAseq" type="boolean" truevalue="--removeShortAAseq" falsevalue="" checked="true" label="Remove short aminoacid sequences" help="This
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299 option exist to allows for easier usage of the Pfam and SignalP web servers which both currently have restrictions on allowed sequence lengths. If
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300 enabled AA sequences are filtered to be > 5 AA. This will only affect the sequences written to the FASTA file not the sequences added to the switchAnalyzeRlist" />
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301 <param argument="removeLongAAseq" type="boolean" truevalue="--removeLongAAseq" falsevalue="" checked="false" label="Remove long aminoacid sequences" help="A
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302 logical indicating whether to removesequences based on their length. This option exist to allows for easier usage of the Pfam and SignalP web servers
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303 which both currently have restrictions on allowed sequence lengths. If enabled AA sequences are filtered to be smaller 1000 AA. This will only affect the
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304 sequences written to the fasta file (if writeToFile=TRUE) not the sequences added to the switchAnalyzeRlist. " />
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305 <param argument="removeORFwithStop" type="boolean" truevalue="--removeORFwithStop" falsevalue="" checked="true" label="Remove ORFs containint STOP codons" help="ORFs
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306 containing stop codons, defined as * when the ORF nucleotide sequences is translated to the amino acid sequence, should be A) removed from the ORF
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307 annotation in the switchAnalyzeRlist and B) removed from the sequences added to the switchAnalyzeRlist and/or written to FASTA files. This is only
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308 necessary if you are analyzing quantified known annotated data where you supplied a GTF file to the import function" />
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309 </section>
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310 <param name="outputs_first" type="select" display="checkboxes" multiple="true" label="Outputs selector">
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311 <option value="nt" selected="true">Nucleotide sequences</option>
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312 <option value="aa" selected="true">Aminoacid sequences</option>
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313 <option value="summary" selected="true">Gene switch summary</option>
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314 </param>
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315 </when>
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316
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317 <!-- WRAPPER SECOND STEP SECTION-->
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318
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319 <when value="second_step">
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320 <param name="robject" type="data" format="rdata" label="IsoformSwitchAnalyzeR R object" help="It is generated when running the analysis part 2." />
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321 <conditional name="analysis_mode">
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322 <param name="selector" type="select" label="Analysis mode" help="This selector allows so specify if you want to analyze a specific gene or
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323 the (top) switching genes/isoforms ">
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324 <option value="top" selected="true">Full analysis</option>
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325 <option value="single">Analyze specific gene</option>
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326 </param>
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327 <when value="top">
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328 <expand macro="macro_alpha_difcutoff"/>
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329 <param argument="n" type="integer" min="1" value="10" label="Number of top switching features (genes/isoforms) to plot"
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330 help="This parameters allows to specify the number of top genes/isoforms to plot"/>
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331 <section name="advanced_options" title="Full analysis advanced options">
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332 <param argument="filterForConsequences" type="boolean" truevalue="--filterForConsequences" falsevalue="" checked="false" label="Filter genes with functional consequences"
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333 help="The output will then be the number of significant genes and isoforms originating from genes with predicted consequences"/>
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334 <param argument="sortByQvals" type="boolean" truevalue="--sortByQvals" falsevalue="" checked="true" label="Sorting mode" help="A logic indicating
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335 whether to the top n features are sorted by decreasing significance (increasing q-values) (if enabled) or decreasing switch size
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336 (absolute dIF, which are still significant as defined by alpha) (if disabled). The dIF values for genes are considered as the total
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
337 change within the gene calculated as sum(abs(dIF)) for each gene" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
338 <expand macro="macro_onlysigisoforms2"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
339 <expand macro="macro_onlyswitching"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
340 <param argument="countGenes" type="boolean" truevalue="--countGenes" falsevalue="" checked="true" label="Number genes or isoform switches counts" help="This parameter indicates
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
341 whether it is the number of genes (if enabled) or isoform switches (if disabled) which primary result in gain/loss that are counted" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
342 <param argument="asFractionTotal" type="boolean" truevalue="--asFractionTotal" falsevalue="" checked="false" label="Summary as numbers of as fraction" help="The consequences/splicing events
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
343 should be summarized calculated as numbers (if disabled) or as a fraction of the total number of switches/genes" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
344 <param argument="plotGenes" type="boolean" truevalue="--plotGenes" falsevalue="" checked="false" label="Plot number/fraction of genes or isoforms" help="Plot the number/fraction of genes with
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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parents:
diff changeset
345 (if enabled) or isoforms (if disabled) involved with isoform switches with functional consequences (both filtered via alpha and dIFcutoff)" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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parents:
diff changeset
346 <param argument="simplifyLocation" type="boolean" truevalue="--simplifyLocation" falsevalue="" checked="true" label="Simplify location" help="Simplify the switches involved in changes
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
347 in subcellular localizations (due the the hundreds of possible combinations)" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
348 <param argument="removeEmptyConsequences" type="boolean" truevalue="--removeEmptyConsequences" falsevalue="" checked="false" label="Remove empty consequences" help="Remove consequenses analyzed
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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parents:
diff changeset
349 but where no differences was found (those showing zero in the plot)" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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parents:
diff changeset
350 <param argument="analysisOppositeConsequence" type="boolean" truevalue="--analysisOppositeConsequence" falsevalue="" checked="false"
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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parents:
diff changeset
351 label="Analysis opposite consequences in enrichment analysis" help="Reverse the analysis meaning if 'Domain gains' are analyze will case the analysis to be performed on 'Domain loss'.
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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parents:
diff changeset
352 The main effect is for the visual appearance of plot which will be mirrored (around the 0.5 fraction)" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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parents:
diff changeset
353 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
354 </when>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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parents:
diff changeset
355 <when value="single">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
356 <param argument="gene" type="text" value="" label="Gene name" help="Either the gene_id or the gene name of the gene to plot">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
357 <sanitizer invalid_char="">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
358 <valid initial="string.letters,string.digits">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
359 <add value="_" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
360 <add value="-" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
361 </valid>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
362 </sanitizer>
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iuc
parents:
diff changeset
363 <validator type="regex">[0-9a-zA-Z_-]+</validator>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
364 </param>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
365 <expand macro="macro_alpha_difcutoff"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
366 <section name="advanced_options" title="Single gene mode advanced options">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
367 <expand macro="macro_ifcutoff" value="0.05" help="The cutoff used for the minimum contribution to gene expression (in at least one
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
368 condition) for an isoforms must have to be plotted (measured as Isoform Fraction (IF) values)" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
369 <param argument="rescaleTranscripts" type="boolean" truevalue="--rescaleTranscripts" falsevalue="" checked="true" label="Rescale transcripts"
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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parents:
diff changeset
370 help="All the isoforms should be resealed to the square root of their original sizes. This feature is implemented because
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
371 introns usually are much larger than exons making it difficult to see structural changes. This is very useful for structural
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
372 visualization but the scaling might distort actual intron and exon sizes" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
373 <param argument="reverseMinus" type="boolean" truevalue="--reverseMinus" falsevalue="" checked="true" label="Isoforms on minus strand
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
374 should be inverted" help="Isoforms on minus strand should be inverted so they are visualized as going from left to right
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
375 instead of right to left" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
376 <param argument="addErrorbars" type="boolean" truevalue="--addErrorbars" falsevalue="" checked="true" label="Add error bars" help="Error
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
377 bars should be added to the expression plots to show uncertainty in estimates" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
378 <expand macro="macro_onlyswitching"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
379 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
380 </when>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
381 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
382 <conditional name="coding_potential">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
383 <param name="selector" type="select" label="Include prediction of coding potential information"
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
384 help="Integrate in the analysis de output from CPAT or CPC2.">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
385 <option value="disabled">Disabled</option>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
386 <option value="cpat">CPAT</option>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
387 <option value="cpc2">CPC2</option>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
388 </param>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
389 <when value="disabled"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
390 <when value="cpat">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
391 <param argument="analyzeCPAT" type="data" format="txt" label="CPAT result file"
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
392 help=" Use default parameters and the nucleotide fasta file (_nt.fasta). If the webserver was used, download the tab-delimited
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
393 result file (available at the bottom of the result page). If a stand-alone version was used, just supply the path to the result file" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
394 <param argument="codingCutoff" type="float" min="0" max="1" value="0.725" label="Coding cutoff" help="cutoff used by CPAT for distinguishing between
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
395 coding and non-coding transcripts. The cutoff is dependent on species analyzed. IsoformSwitchAnalyzerR developers suggest that the optimal cutoff
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
396 for overlapping coding and noncoding isoforms are 0.725 for human and 0.721 for mouse. However the suggested cutoffs from the CPAT develpers
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
397 derived by comparing known genes to random non-coding regions of the genome is 0.364 for human and 0.44 for mouse" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
398 </when>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
399 <when value="cpc2">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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400 <param argument="analyzeCPC2" type="data" format="txt" label="CPC2 result file"
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
401 help="Use default parameters and if required select the most similar species. If the webserver (batch submission) was used,
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
402 download the tab-delimited result file (via the “Download the result” button). If a stand-alone version was just just supply the path to the result file" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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parents:
diff changeset
403 <param argument="removeNoncodingORFs" type="boolean" truevalue="--removeNoncodingORFs" falsevalue="" checked="false" label="Remove non-coding ORFs" help="Remove ORF information
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
404 from the isoforms which the CPC2 analysis classifies as non-coding. This can be particular useful if the isoform (and ORF) was predicted de-novo but is not
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
405 recommended if ORFs was imported from a GTF file" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
406 <param argument="codingCutoff" type="float" min="0" max="1" value="0.5" label="Coding cutoff" help="Numeric indicating the cutoff used by CPC2 for distinguishing
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
407 between coding and non-coding transcripts. The cutoff appears to be species independent." />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
408 </when>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
409 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
410 <conditional name="protein_domains">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
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diff changeset
411 <param name="selector" type="select" label="Include Pfam information" help="Pfam is a database of protein families that includes their annotations and multiple sequence
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
412 alignments generated using hidden Markov models.">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
413 <option value="disabled">Disabled</option>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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parents:
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414 <option value="enabled">Enabled</option>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
415 </param>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
416 <when value="disabled"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
417 <when value="enabled">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
418 <param argument="analyzePFAM" type="data" format="txt" multiple="true" optional="true" label="Include Pfam results (sequence analysis of protein domains)" help="Use default
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
419 parameters and the amino acid fasta file (_AA.fasta). If the webserver is used you need to copy/paste the result part of the mail you receive into an empty plain text
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
420 document (notepad, sublimetext, TextEdit or similar (not Word)) and save that to a plain text (txt) file. The path to that file should be supplied. If a stand-alone
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
421 version was used, just supply the path to the result file" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
422 </when>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
423 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
424 <conditional name="signal_peptides">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
425 <param name="selector" type="select" label="Include SignalP results" help="Integration of the result of SignalP (external sequence analysis of signal peptides)">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
426 <option value="disabled">Disabled</option>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
427 <option value="enabled">Enabled</option>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
428 </param>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
429 <when value="disabled"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
430 <when value="enabled">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
431 <param argument="analyzeSignalP" type="data" format="txt" multiple="true" optional="true" label="SignalP" help="Use the amino acid fasta file (_AA.fasta). If using the webserver SignalP
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
432 should be run with the parameter 'Short output (no figures)' under 'Output format' and one should select the appropriate 'Organism group'. When
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
433 using a stand-alone version SignalP should be run with the '-f summary' option. If using the webserver the results can be downloaded using the
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
434 'Downloads' button in the top-right corner where the user should select 'Prediction summary' and supply the path to the resulting file to the
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
435 'pathToSignalPresultFile' argument. If a stand-alone version was just supply the path to the summary result file." />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
436 <param argument="minSignalPeptideProbability" type="float" min="0" max="1" value="0.5" label="Minimum probability for calling a signal peptide"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
437 </when>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
438 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
439
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
440 <conditional name="disordered_regions">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
441 <param name="selector" type="select" label="Include prediction of intrinsically disordered Regions (IDR) information"
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
442 help="Integrate in the analysis de output from IUPred2A or NetSurfP-2">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
443 <option value="disabled">Disabled</option>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
444 <option value="iupred2a">IUPred2A</option>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
445 <option value="netsurfp">NetSurfP-2</option>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
446 </param>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
447 <when value="disabled"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
448 <when value="iupred2a">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
449 <param argument="AanalyzeIUPred2A" type="data" format="txt,gz" label="IUPred2A result file" help="Can be gziped. If
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
450 multiple result files were created (multiple web-server runs) just supply all of them." />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
451 <expand macro="macro_disordered_regions"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
452 <param argument="annotateBindingSites" type="boolean" truevalue="--annotateBindingSites" falsevalue="" checked="true" label="Annotate binding sites"
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
453 help="Integrate the ANCHOR2 prediction of Intrinsically Disordered Binding Regions (IDBRs)" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
454 <param argument="minIdrBindingSize" type="integer" min="0" value="15" label="Minimum IDBR binding size" help="How long a stretch of
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
455 binding site the region part of the Intrinsically Disordered Binding Regions (IDBR)" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
456 <param argument="minIdrBindingOverlapFrac" type="float" min="0" value="0.8" label="Minimum fraction of a predicted IDBR" help="Minimum
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
457 fraction of a predicted IDBR must also be within a IDR before the IDR is considered as a an IDR with a binding region" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
458 </when>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
459 <when value="netsurfp">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
460 <param argument="analyzeNetSurfP2" type="data" format="txt,gz" multiple="true" label="NetSurfP-2 result file" help="Can be gziped. If
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
461 multiple result files were created (multiple web-server runs) just supply all of them." />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
462 <expand macro="macro_disordered_regions"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
463 </when>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
464 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
465 <section name="analyzeSwitchConsequences" title="Analyze switch consequences parameters">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
466 <param argument="ntCutoff" type="integer" min="0" value="50" label="Nucleotide length cutoff" help="The length difference (in nucleotides) a
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
467 comparison must be larger than for reporting differences" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
468 <param argument="ntFracCutoff" type="float" min="0" max="1" optional="true" label="Nucleotide length fraction cutoff" help="The cutoff in length
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
469 difference, measured as a fraction of the length of the downregulated isoform, a comparison must be larger than for
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
470 reporting differences. For example does 0.05 mean the upregulated isoform must be 5% longer/shorter before it is reported. " />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
471 <param argument="ntJCsimCutoff" type="float" min="0" max="1" value="0.8" label="Cutoff on Jaccard similarity between the overlap of two nucloetide sequences" help=" If the
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
472 measured JCsim is smaller than this cutoff the sequences are considered different and reported as such" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
473 <param argument="AaCutoff" type="integer" min="0" value="10" label="Aminoacid lenght cutoff" help="Length difference (in AA) a comparison must be larger
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
474 than for reporting differences when evaluating ’ORF_seq_similarity’, primarily implemented to avoid differences in very short AA sequences being
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
475 classified as different" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
476 <param argument="AaFracCutoff" type="float" min="0" max="1" value="0.5" label="Aminoacid length fraction cutoff" help="Cutoff of length difference of
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
477 the protein domain or IDR. The difference is measured as a fraction of the longest region, a comparison must be larger than before reporting it" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
478 <param argument="AaJCsimCutoff" type="float" min="0" max="1" value="0.9" label="Cutoff between the overlap of two aminoacid sequences" help="If the
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
479 measured JCsim is smaller than this cutoff the sequences are considered different and reported as such" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
480 <param argument="removeNonConseqSwitches" type="boolean" truevalue="--removeNonConseqSwitches" falsevalue="" checked="true" label="Remove the comparison of isoforms
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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diff changeset
481 where no consequences were found" />
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parents:
diff changeset
482 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
483 </when>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
484 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
485 </inputs>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
486 <outputs>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
487 <collection name="collection_counts_factor1" type="list" label="${tool.name} on ${on_string}: gene counts factor1">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
488 <discover_datasets pattern="__designation_and_ext__" format="tabular" directory="count_files/factor1" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
489 <filter>functionMode['selector'] == 'data_import'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
490 <filter>functionMode['countFiles'] == 'collection'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
491 </collection>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
492 <collection name="collection_counts_factor2" type="list" label="${tool.name} on ${on_string}: gene counts factor2">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
493 <discover_datasets pattern="__designation_and_ext__" format="tabular" directory="count_files/factor2" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
494 <filter>functionMode['selector'] == 'data_import'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
495 <filter>functionMode['countFiles'] == 'collection'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
496 </collection>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
497 <data name="matrix_counts" format="tabular" from_work_dir="count_files/matrix.tabular" label="${tool.name} on ${on_string}: gene counts matrix">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
498 <filter>functionMode['selector'] == 'data_import'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
499 <filter>functionMode['countFiles'] == 'matrix'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
500 </data>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
501 <data name="sample_annotation" format="tabular" from_work_dir="count_files/samples.tabular" label="${tool.name} on ${on_string}: samples annotation">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
502 <filter>functionMode['selector'] == 'data_import'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
503 <filter>functionMode['countFiles'] == 'matrix'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
504 </data>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
505 <data name="switchList" format="rdata" from_work_dir="SwitchList.Rda" label="${tool.name} on ${on_string}: SwitchList (RData)"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
506 <data name="isoformAA" format="fasta" from_work_dir="isoformSwitchAnalyzeR_isoform_AA.fasta" label="${tool.name} on ${on_string}: aminoacid sequences">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
507 <filter>functionMode['selector'] == 'first_step'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
508 <filter>functionMode['outputs_first'] and 'aa' in functionMode['outputs_first']</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
509 </data>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
510 <data name="isoformNT" format="fasta" from_work_dir="isoformSwitchAnalyzeR_isoform_nt.fasta" label="${tool.name} on ${on_string}: nucleotide sequences">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
511 <filter>functionMode['selector'] == 'first_step'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
512 <filter>functionMode['outputs_first'] and 'nt' in functionMode['outputs_first']</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
513 </data>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
514 <data name="switchSummary" format="tabular" from_work_dir="switchSummary.tsv" label="${tool.name} on ${on_string}: summary">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
515 <filter>functionMode['selector'] == 'first_step'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
516 <filter>functionMode['outputs_first'] and 'summary' in functionMode['outputs_first']</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
517 </data>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
518 <collection name="plots_summary" type="list" label="${tool.name} on ${on_string}: genome wide plots">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
519 <discover_datasets pattern="__designation_and_ext__" format="pdf" directory="pdf_outputs" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
520 <filter>functionMode['selector'] == 'second_step'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
521 <filter>functionMode['analysis_mode']['selector'] == 'top'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
522 </collection>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
523 <collection name="genes_consequences" type="list" label="${tool.name} on ${on_string}: isoform switches with predicted functional consequences plots">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
524 <discover_datasets pattern="__designation_and_ext__" format="pdf" directory="gene_plots/with_consequences" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
525 <filter>functionMode['selector'] == 'second_step'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
526 <filter>functionMode['analysis_mode']['selector'] == 'top'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
527 </collection>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
528 <collection name="genes_wo_consequences" type="list" label="${tool.name} on ${on_string}: isoform switches without predicted functional consequences plots">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
529 <discover_datasets pattern="__designation_and_ext__" format="pdf" directory="gene_plots/without_consequences" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
530 <filter>functionMode['selector'] == 'second_step'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
531 <filter>functionMode['analysis_mode']['selector'] == 'top'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
532 </collection>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
533 <data name="mostSwitching" format="tabular" from_work_dir="mostSwitchingGene.tsv" label="${tool.name} on ${on_string}: switching gene/isoforms">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
534 <filter>functionMode['selector'] == 'second_step'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
535 <filter>functionMode['analysis_mode']['selector'] == 'top'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
536 </data>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
537 <data name="consequencesSummary" format="tabular" from_work_dir="consequencesSummary.tsv" label="${tool.name} on ${on_string}: consequences summary">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
538 <filter>functionMode['selector'] == 'second_step'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
539 <filter>functionMode['analysis_mode']['selector'] == 'top'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
540 </data>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
541 <data name="consequencesEnrichment" format="tabular" from_work_dir="consequencesEnrichment.tsv" label="${tool.name} on ${on_string}: consequences enrichment">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
542 <filter>functionMode['selector'] == 'second_step'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
543 <filter>functionMode['analysis_mode']['selector'] == 'top'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
544 </data>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
545 <data name="splicingSummary" format="tabular" from_work_dir="splicingSummary.tsv" label="${tool.name} on ${on_string}: splicing summary">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
546 <filter>functionMode['selector'] == 'second_step'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
547 <filter>functionMode['analysis_mode']['selector'] == 'top'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
548 </data>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
549 <data name="splicingEnrichment" format="tabular" from_work_dir="splicingEnrichment.tsv" label="${tool.name} on ${on_string}: splicing enrichment">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
550 <filter>functionMode['selector'] == 'second_step'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
551 <filter>functionMode['analysis_mode']['selector'] == 'top'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
552 </data>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
553 <data name="single_gene" format="pdf" from_work_dir="single_gene.pdf" label="${tool.name} on ${on_string}: single gene analysis">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
554 <filter>functionMode['selector'] == 'second_step'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
555 <filter>functionMode['analysis_mode']['selector'] == 'single'</filter>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
556 </data>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
557 </outputs>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
558 <tests>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
559 <!-- Test 01: Data import mode-->
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
560 <test expect_num_outputs="1">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
561 <conditional name="functionMode">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
562 <param name="selector" value="data_import"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
563 <param name="genomeAnnotation" value="annotation_salmon.gtf.gz"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
564 <param name="transcriptome" value="transcriptome.fasta.gz"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
565 <param name="countFiles" value="disabled"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
566 <section name="first_factor">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
567 <param name="factorLevel" value="health"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
568 <param name="trans_counts" value="salmon_cond1_rep1.sf,salmon_cond1_rep2.sf"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
569 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
570 <section name="second_factor">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
571 <param name="factorLevel" value="cancer"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
572 <param name="trans_counts" value="salmon_cond2_rep1.sf,salmon_cond2_rep2.sf"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
573 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
574 <conditional name="tool_source">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
575 <param name="selector" value="salmon"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
576 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
577 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
578 <output name="switchList" file="test01.RData" ftype="rdata" compare="sim_size" delta="100"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
579 </test>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
580 <!-- Test 02: Data import mode generate expression matrix-->
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
581 <test expect_num_outputs="3">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
582 <conditional name="functionMode">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
583 <param name="selector" value="data_import"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
584 <param name="genomeAnnotation" value="annotation_salmon.gtf.gz"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
585 <param name="transcriptome" value="transcriptome.fasta.gz"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
586 <param name="countFiles" value="matrix"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
587 <section name="first_factor">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
588 <param name="factorLevel" value="health"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
589 <param name="trans_counts" value="salmon_cond1_rep1.sf,salmon_cond1_rep2.sf"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
590 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
591 <section name="second_factor">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
592 <param name="factorLevel" value="cancer"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
593 <param name="trans_counts" value="salmon_cond2_rep1.sf,salmon_cond2_rep2.sf"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
594 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
595 <conditional name="tool_source">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
596 <param name="selector" value="salmon"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
597 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
598 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
599 <output name="switchList" ftype="rdata">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
600 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
601 <has_size value="652170" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
602 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
603 </output>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
604 <output name="matrix_counts" file="test02_counts.tabular" ftype="tabular" lines_diff="6"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
605 <output name="sample_annotation" file="test02_samples_annotation.tabular" ftype="tabular"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
606 </test>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
607 <!-- Test 03: Data import mode generate collection count files-->
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
608 <test expect_num_outputs="3">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
609 <conditional name="functionMode">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
610 <param name="selector" value="data_import"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
611 <param name="genomeAnnotation" value="annotation_salmon.gtf.gz"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
612 <param name="transcriptome" value="transcriptome.fasta.gz"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
613 <param name="countFiles" value="collection"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
614 <section name="first_factor">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
615 <param name="factorLevel" value="health"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
616 <param name="trans_counts" value="salmon_cond1_rep1.sf,salmon_cond1_rep2.sf"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
617 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
618 <section name="second_factor">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
619 <param name="factorLevel" value="cancer"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
620 <param name="trans_counts" value="salmon_cond2_rep1.sf,salmon_cond2_rep2.sf"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
621 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
622 <conditional name="tool_source">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
623 <param name="selector" value="salmon"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
624 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
625 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
626 <output name="switchList" ftype="rdata">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
627 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
628 <has_size value="652170" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
629 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
630 </output>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
631 <output_collection name="collection_counts_factor1" type="list" count="2">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
632 <element name="cancer0_dataset" file="test03_cancer_counts.tabular" ftype="tabular" lines_diff="6"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
633 </output_collection>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
634 <output_collection name="collection_counts_factor2" type="list" count="2">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
635 <element name="health0_dataset" file="test03_health_counts.tabular" ftype="tabular" lines_diff="6"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
636 </output_collection>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
637 </test>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
638 <!-- Test 04: Extract isoform switches all outputs-->
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
639 <test expect_num_outputs="4">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
640 <conditional name="functionMode">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
641 <param name="selector" value="first_step"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
642 <param name="robject" value="test01.RData"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
643 <param name="alpha" value="0.05"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
644 <param name="dIFcutoff" value="0.1"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
645 <param name="onlySigIsoforms" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
646 <param name="filterForConsequences" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
647 <param name="outputs_first" value="nt,aa,summary"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
648 <section name="prefilter">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
649 <param name="geneExpressionCutoff" value="1"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
650 <param name="isoformExpressionCutoff" value="0"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
651 <param name="IFcutoff" value="0.01"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
652 <param name="removeSingleIsformGenes" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
653 <param name="keepIsoformInAllConditions" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
654 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
655 <section name="dexseq">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
656 <param name="correctForConfoundingFactors" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
657 <param name="overwriteIFvalues" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
658 <param name="reduceToSwitchingGenes" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
659 <param name="reduceFurtherToGenesWithConsequencePotential" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
660 <param name="keepIsoformInAllConditions" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
661 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
662 <section name="novel_isoform">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
663 <param name="minORFlength" value="100"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
664 <param name="orfMethod" value="longest.AnnotatedWhenPossible"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
665 <param name="PTCDistance" value="50"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
666 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
667 <section name="extract_sequence">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
668 <param name="onlySwitchingGenes" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
669 <param name="removeShortAAseq" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
670 <param name="removeLongAAseq" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
671 <param name="removeORFwithStop" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
672 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
673 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
674 <output name="switchList" file="test04.RData" ftype="rdata" compare="sim_size" delta="100"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
675 <output name="isoformAA" ftype="fasta">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
676 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
677 <has_size value="138275" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
678 <has_text text=">TCONS_00000007"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
679 <has_text text="MLLPPGSLSRPRTFSSQPLQT"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
680 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
681 </output>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
682 <output name="isoformNT" ftype="fasta">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
683 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
684 <has_size value="780375" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
685 <has_text text=">TCONS_00000007"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
686 <has_text text="GGGTCTCCCTCTGTTGTCCAAGGC"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
687 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
688 </output>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
689 <output name="switchSummary" file="test04_summary.tabular" ftype="tabular"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
690 </test>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
691 <!-- Test 05: Extract isoform switches alternative parameters-->
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
692 <test expect_num_outputs="1">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
693 <conditional name="functionMode">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
694 <param name="selector" value="first_step"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
695 <param name="robject" value="test01.RData"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
696 <param name="outputs_first" value=""/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
697 <section name="dexseq">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
698 <param name="correctForConfoundingFactors" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
699 <param name="overwriteIFvalues" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
700 <param name="reduceToSwitchingGenes" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
701 <param name="reduceFurtherToGenesWithConsequencePotential" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
702 <param name="keepIsoformInAllConditions" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
703 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
704 <section name="novel_isoform">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
705 <param name="orfMethod" value="mostUpstream"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
706 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
707 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
708 <output name="switchList" ftype="rdata">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
709 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
710 <has_size value="500518" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
711 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
712 </output>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
713 </test>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
714 <!--Test 06: generate plots and summaries full analsys-->
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
715 <test expect_num_outputs="9">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
716 <conditional name="functionMode">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
717 <param name="selector" value="second_step"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
718 <param name="robject" value="test04.RData"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
719 <section name="analyzeSwitchConsequences">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
720 <param name="ntCutoff" value="50"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
721 <param name="ntJCsimCutoff" value="0.8"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
722 <param name="AaCutoff" value="10"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
723 <param name="AaFracCutoff" value="0.5"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
724 <param name="AaJCsimCutoff" value="0.9"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
725 <param name="removeNonConseqSwitches" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
726 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
727 <conditional name="analysis_mode">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
728 <param name="selector" value="top"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
729 <param name="alpha" value="0.05"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
730 <param name="dIFcutoff" value="0.1"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
731 <param name="n" value="2"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
732 <section name="advanced_options">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
733 <param name="filterForConsequences" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
734 <param name="sortByQvals" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
735 <param name="onlySigIsoforms" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
736 <param name="onlySwitchingGenes" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
737 <param name="countGenes" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
738 <param name="asFractionTotal" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
739 <param name="plotGenes" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
740 <param name="simplifyLocation" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
741 <param name="removeEmptyConsequences" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
742 <param name="analysisOppositeConsequence" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
743 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
744 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
745 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
746 <output name="switchList" ftype="rdata">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
747 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
748 <has_size value="531580" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
749 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
750 </output>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
751 <output_collection name="plots_summary" type="list" count="7">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
752 <element name="consequencesEnrichment" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
753 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
754 <has_size value="5995" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
755 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
756 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
757 <element name="extractConsequencesSummary" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
758 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
759 <has_size value="5681" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
760 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
761 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
762 <element name="splicingEnrichment" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
763 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
764 <has_size value="6361" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
765 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
766 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
767 <element name="splicingGenomewide" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
768 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
769 <has_size value="68069" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
770 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
771 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
772 <element name="splicingSummary" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
773 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
774 <has_size value="5990" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
775 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
776 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
777 <element name="switchGene" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
778 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
779 <has_size value="3611" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
780 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
781 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
782 <element name="volcanoPlot" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
783 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
784 <has_size value="3611" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
785 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
786 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
787 </output_collection>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
788 <output_collection name="genes_consequences" type="list" count="2">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
789 <element name="1_switch_plot_NADK_aka_NADK" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
790 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
791 <has_size value="8716" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
792 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
793 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
794 <element name="2_switch_plot_PRKCZ_aka_PRKCZ" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
795 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
796 <has_size value="8463" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
797 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
798 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
799 </output_collection>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
800 <output_collection name="genes_wo_consequences" type="list" count="2">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
801 <element name="1_switch_plot_CLSTN1_aka_CLSTN1" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
802 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
803 <has_size value="8039" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
804 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
805 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
806 <element name="2_switch_plot_ZBTB40_aka_ZBTB40" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
807 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
808 <has_size value="7506" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
809 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
810 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
811 </output_collection>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
812 <output name="mostSwitching" file="test06_switching.tabular" ftype="tabular" lines_diff="4"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
813 <output name="consequencesSummary" file="test06_consequences_summary.tabular" ftype="tabular" lines_diff="4"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
814 <output name="consequencesEnrichment" file="test06_consequences_enrichment.tabular" ftype="tabular" lines_diff="4"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
815 <output name="splicingSummary" file="test06_splicing_summary.tabular" ftype="tabular" lines_diff="4"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
816 <output name="splicingEnrichment" file="test06_splicing_enrichment.tabular" ftype="tabular" lines_diff="4"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
817 </test>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
818 <!--Test 07: generate plots and summaries full analsys all inputs-->
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
819 <test expect_num_outputs="9">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
820 <conditional name="functionMode">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
821 <param name="selector" value="second_step"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
822 <param name="robject" value="test04.RData"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
823 <section name="analyzeSwitchConsequences">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
824 <param name="ntCutoff" value="20"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
825 <param name="ntJCsimCutoff" value="0.5"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
826 <param name="AaCutoff" value="10"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
827 <param name="AaFracCutoff" value="0.4"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
828 <param name="AaJCsimCutoff" value="0.8"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
829 <param name="removeNonConseqSwitches" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
830 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
831 <conditional name="analysis_mode">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
832 <param name="selector" value="top"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
833 <param name="alpha" value="0.05"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
834 <param name="dIFcutoff" value="0.1"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
835 <param name="n" value="2"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
836 <section name="advanced_options">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
837 <param name="filterForConsequences" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
838 <param name="sortByQvals" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
839 <param name="onlySigIsoforms" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
840 <param name="onlySwitchingGenes" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
841 <param name="countGenes" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
842 <param name="asFractionTotal" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
843 <param name="plotGenes" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
844 <param name="simplifyLocation" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
845 <param name="removeEmptyConsequences" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
846 <param name="analysisOppositeConsequence" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
847 </section>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
848 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
849 <conditional name="coding_potential">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
850 <param name="selector" value="cpc2"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
851 <param name="analyzeCPC2" value="cpc2_result.txt"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
852 <param name="removeNoncodingORFs" value="false"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
853 <param name="codingCutoff" value="0.5"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
854 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
855 <conditional name="protein_domains">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
856 <param name="selector" value="enabled"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
857 <param name="analyzePFAM" value="pfam_results.txt"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
858 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
859 <conditional name="signal_peptides">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
860 <param name="selector" value="enabled"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
861 <param name="analyzeSignalP" value="signalP_results.txt"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
862 <param name="minSignalPeptideProbability" value="0.5"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
863 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
864 <conditional name="disordered_regions">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
865 <param name="selector" value="iupred2a"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
866 <param name="AanalyzeIUPred2A" value="iupred2a_result.txt.gz"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
867 <param name="smoothingWindowSize" value="5"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
868 <param name="probabilityCutoff" value="0.5"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
869 <param name="minIdrSize" value="30"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
870 <param name="annotateBindingSites" value="true"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
871 <param name="minIdrBindingSize" value="15"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
872 <param name="minIdrBindingOverlapFrac" value="0.8"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
873 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
874 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
875 <output name="switchList" ftype="rdata">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
876 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
877 <has_size value="542120" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
878 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
879 </output>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
880 <output_collection name="plots_summary" type="list" count="7">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
881 <element name="consequencesEnrichment" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
882 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
883 <has_size value="5995" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
884 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
885 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
886 <element name="extractConsequencesSummary" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
887 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
888 <has_size value="6617" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
889 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
890 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
891 <element name="splicingEnrichment" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
892 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
893 <has_size value="6361" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
894 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
895 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
896 <element name="splicingGenomewide" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
897 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
898 <has_size value="68069" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
899 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
900 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
901 <element name="splicingSummary" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
902 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
903 <has_size value="5990" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
904 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
905 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
906 <element name="switchGene" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
907 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
908 <has_size value="3611" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
909 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
910 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
911 <element name="volcanoPlot" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
912 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
913 <has_size value="3611" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
914 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
915 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
916 </output_collection>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
917 <output_collection name="genes_consequences" type="list" count="2">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
918 <element name="1_switch_plot_NADK_aka_NADK" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
919 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
920 <has_size value="8716" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
921 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
922 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
923 <element name="2_switch_plot_PRKCZ_aka_PRKCZ" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
924 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
925 <has_size value="8463" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
926 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
927 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
928 </output_collection>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
929 <output_collection name="genes_wo_consequences" type="list" count="2">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
930 <element name="1_switch_plot_CLSTN1_aka_CLSTN1" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
931 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
932 <has_size value="8559" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
933 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
934 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
935 <element name="2_switch_plot_ZBTB40_aka_ZBTB40" ftype="pdf">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
936 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
937 <has_size value="8051" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
938 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
939 </element>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
940 </output_collection>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
941 <output name="mostSwitching" ftype="tabular">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
942 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
943 <has_size value="4062" delta="50"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
944 <has_text text="RPL11"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
945 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
946 </output>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
947 <output name="consequencesSummary" ftype="tabular">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
948 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
949 <has_size value="1192" delta="50"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
950 <has_text text="nrGenesWithConsequences"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
951 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
952 </output>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
953 <output name="consequencesEnrichment" ftype="tabular">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
954 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
955 <has_size value="1432" delta="50"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
956 <has_text text="NMD insensitive (paired with NMD sensitive"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
957 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
958 </output>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
959 <output name="splicingSummary" ftype="tabular">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
960 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
961 <has_size value="892" delta="50"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
962 <has_text text="MEE in isoform used less"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
963 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
964 </output>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
965 <output name="splicingEnrichment" ftype="tabular">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
966 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
967 <has_size value="1157" delta="50"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
968 <has_text text="A5 gain (paired with A5 loss)"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
969 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
970 </output>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
971 </test>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
972 <!-- Test 08: analyze single gene-->
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
973 <test expect_num_outputs="2">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
974 <conditional name="functionMode">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
975 <param name="selector" value="second_step"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
976 <param name="robject" value="test04.RData"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
977 <conditional name="analysis_mode">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
978 <param name="selector" value="single"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
979 <param name="gene" value="NADK"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
980 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
981 </conditional>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
982 <output name="single_gene" ftype="pdf" file="test08_single_gene.pdf" compare="sim_size"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
983 <output name="switchList" ftype="rdata">
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
984 <assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
985 <has_size value="531580" delta="300"/>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
986 </assert_contents>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
987 </output>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
988 </test>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
989 </tests>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
990 <help><![CDATA[
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
991
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
992 .. class:: infomark
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
993
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
994 **Purpose**
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
995
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
996 IsoformSwitchAnalyzeR is an easy-to use-R package that enables statistical identification of isoform switching from RNA-seq derived quantification
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
997 of novel and/or annotated full-length isoforms. IsoformSwitchAnalyzeR facilitates integration of many sources of (predicted) annotation such as Open
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
998 Reading Frame (ORF/CDS), protein domains (via Pfam), signal peptides (via SignalP), Intrinsically Disordered Regions (IDR, via NetSurfP-2 or IUPred2A),
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
999 coding potential (via CPAT or CPC2) and sensitivity to Non-sense Mediated Decay (NMD) and more. The combination of identified isoform switches and
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1000 their annotation enables IsoformSwitchAnalyzeR to predict potential functional consequences of the identified isoform switches — such as loss of
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1001 protein domains — thereby identifying isoform switches of particular interest. Lastly, IsoformSwitchAnalyzeR provides article-ready visualization
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1002 methods for isoform switches for individual genes as well as both summary statistics and visualization of the genome-wide changes/consequences of
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1003 isoform switches, their consequences and the associated alternative splicing.
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1004
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1005 -----
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1006
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1007 .. class:: infomark
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1008
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1009 **Differential isoform expression (DIE) and differential isoform usage (DIU)**
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1010
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1011 Differential isoform expression (DIE) and differential isoform usage (DIU) are related but distinct concepts. DIE assesses the difference of
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1012 absolute expression in isoform level. In contrast, DIU assesses the difference of relative expression in isoform level. For example, if the
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1013 expression of two isoforms of one gene are 10 and 20 in control and 50 and 100 in case, then there is DIE but no DIU because the relative
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1014 expression of the first isoform is 1/3 in both case and control.
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1015
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iuc
parents:
diff changeset
1016 -----
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1017
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1018 .. class:: infomark
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1019
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1020 **ORF identification methods (novel isoform analysis)**
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1021
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1022 - **Longest**: Identifies the longest ORF in the transcript (after filtering via minORFlength). This approach is similar to what the CPAT tool uses in it is analysis of coding potential.
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1023 - **LongestAnnotated**: Identifies the longest ORF (after filtering via minORFlength) downstream of an annotated translation start site (which are supplied via the cds argument).
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1024 - **Longest.AnnotatedWhenPossible**: A merge between "longestAnnotated" and "longest". For all isoforms where CDS start positions from known isoform overlap, only these CDS starts are considered and the longest ORF is annotated (similar to "longestAnnotated"). All isoforms without any overlapping CDS start sites they will be analysed with the "longest" approach.
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1025 - **MostUpstream**: Identifies the most upstream ORF in the transcript (after filtering via minORFlength).
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
diff changeset
1026 - **MostUpstreamAnnoated**: Identifies the ORF (after filtering via minORFlength) downstream of the most upstream overlapping annotated translation start site (supplied via the cds argument).
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
iuc
parents:
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1027
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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1028 ]]></help>
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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1029 <expand macro="citations" />
bb611fa3bc3b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380
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1030 </tool>