##fileformat=VCFv4.2
##FORMAT=<ID=AD,Number=R,Type=Integer,Description="Allelic depths for the ref and alt alleles in the order listed">
##FORMAT=<ID=AF,Number=A,Type=Float,Description="Allele fractions of alternate alleles in the tumor">
##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Approximate read depth (reads with MQ=255 or with bad mates are filtered)">
##FORMAT=<ID=F1R2,Number=R,Type=Integer,Description="Count of reads in F1R2 pair orientation supporting each allele">
##FORMAT=<ID=F2R1,Number=R,Type=Integer,Description="Count of reads in F2R1 pair orientation supporting each allele">
##FORMAT=<ID=FAD,Number=R,Type=Integer,Description="Count of fragments supporting each allele.">
##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
##FORMAT=<ID=PGT,Number=1,Type=String,Description="Physical phasing haplotype information, describing how the alternate alleles are phased in relation to one another; will always be heterozygous and is not intended to describe called alleles">
##FORMAT=<ID=PID,Number=1,Type=String,Description="Physical phasing ID information, where each unique ID within a given sample (but not across samples) connects records within a phasing group">
##FORMAT=<ID=PL,Number=G,Type=Integer,Description="Normalized, Phred-scaled likelihoods for genotypes as defined in the VCF specification">
##FORMAT=<ID=PS,Number=1,Type=Integer,Description="Phasing set (typically the position of the first variant in the set)">
##FORMAT=<ID=SB,Number=4,Type=Integer,Description="Per-sample component statistics which comprise the Fisher's Exact Test to detect strand bias.">
##GATKCommandLine=<ID=Mutect2,CommandLine="Mutect2 --tumor-sample SRR8525881 --output output.vcf --input tumor.bam --reference reference.fa --QUIET true --f1r2-median-mq 50 --f1r2-min-bq 20 --f1r2-max-depth 200 --flow-likelihood-parallel-threads 0 --flow-likelihood-optimized-comp false --trim-to-haplotype true --exact-matching false --flow-use-t0-tag false --flow-remove-non-single-base-pair-indels false --flow-remove-one-zero-probs false --flow-quantization-bins 121 --flow-fill-empty-bins-value 0.001 --flow-symmetric-indel-probs false --flow-report-insertion-or-deletion false --flow-disallow-probs-larger-than-call false --flow-lump-probs false --flow-retain-max-n-probs-base-format false --flow-probability-scaling-factor 10 --flow-order-cycle-length 4 --keep-boundary-flows false --genotype-pon-sites false --genotype-germline-sites false --genotype-germline-sites-fraction 1.0 --af-of-alleles-not-in-resource -1.0 --mitochondria-mode false --permutect-ref-downsample 10 --permutect-alt-downsample 20 --permutect-non-artifact-ratio 1 --permutect-dataset-mode ILLUMINA --tumor-lod-to-emit 3.0 --initial-tumor-lod 2.0 --pcr-snv-qual 40 --pcr-indel-qual 40 --base-qual-correction-factor 5 --max-population-af 0.01 --downsampling-stride 1 --callable-depth 10 --max-suspicious-reads-per-alignment-start 0 --normal-lod 2.2 --ignore-itr-artifacts false --gvcf-lod-band -2.5 --gvcf-lod-band -2.0 --gvcf-lod-band -1.5 --gvcf-lod-band -1.0 --gvcf-lod-band -0.5 --gvcf-lod-band 0.0 --gvcf-lod-band 0.5 --gvcf-lod-band 1.0 --minimum-allele-fraction 0.0 --independent-mates false --flow-mode NONE --disable-adaptive-pruning false --kmer-size 10 --kmer-size 25 --dont-increase-kmer-sizes-for-cycles false --allow-non-unique-kmers-in-ref false --num-pruning-samples 1 --min-dangling-branch-length 4 --recover-all-dangling-branches false --max-num-haplotypes-in-population 128 --min-pruning 2 --adaptive-pruning-initial-error-rate 0.001 --pruning-lod-threshold 2.302585092994046 --pruning-seeding-lod-threshold 9.210340371976184 --max-unpruned-variants 100 --linked-de-bruijn-graph false --disable-artificial-haplotype-recovery false --enable-legacy-graph-cycle-detection false --debug-assembly false --debug-graph-transformations false --capture-assembly-failure-bam false --num-matching-bases-in-dangling-end-to-recover -1 --error-correction-log-odds -Infinity --error-correct-reads false --kmer-length-for-read-error-correction 25 --min-observations-for-kmer-to-be-solid 20 --likelihood-calculation-engine PairHMM --base-quality-score-threshold 18 --dragstr-het-hom-ratio 2 --dont-use-dragstr-pair-hmm-scores false --pair-hmm-gap-continuation-penalty 10 --expected-mismatch-rate-for-read-disqualification 0.02 --pair-hmm-implementation FASTEST_AVAILABLE --pcr-indel-model CONSERVATIVE --phred-scaled-global-read-mismapping-rate 45 --disable-symmetric-hmm-normalizing false --disable-cap-base-qualities-to-map-quality false --enable-dynamic-read-disqualification-for-genotyping false --dynamic-read-disqualification-threshold 1.0 --native-pair-hmm-threads 4 --native-pair-hmm-use-double-precision false --flow-hmm-engine-min-indel-adjust 6 --flow-hmm-engine-flat-insertion-penatly 45 --flow-hmm-engine-flat-deletion-penatly 45 --pileup-detection false --use-pdhmm false --use-pdhmm-overlap-optimization false --make-determined-haps-from-pd-code false --print-pileupcalling-status false --fallback-gga-if-pdhmm-fails true --pileup-detection-enable-indel-pileup-calling false --pileup-detection-active-region-phred-threshold 0.0 --num-artificial-haplotypes-to-add-per-allele 5 --artifical-haplotype-filtering-kmer-size 10 --pileup-detection-snp-alt-threshold 0.1 --pileup-detection-indel-alt-threshold 0.1 --pileup-detection-absolute-alt-depth 0.0 --pileup-detection-snp-adjacent-to-assembled-indel-range 5 --pileup-detection-snp-basequality-filter 12 --pileup-detection-bad-read-tolerance 0.0 --pileup-detection-proper-pair-read-badness true --pileup-detection-edit-distance-read-badness-threshold 0.08 --pileup-detection-chimeric-read-badness true --pileup-detection-template-mean-badness-threshold 0.0 --pileup-detection-template-std-badness-threshold 0.0 --pileup-detection-filter-assembly-alt-bad-read-tolerance 0.0 --pileup-detection-edit-distance-read-badness-for-assembly-filtering-threshold 0.12 --bam-writer-type CALLED_HAPLOTYPES --dont-use-soft-clipped-bases false --override-fragment-softclip-check false --min-base-quality-score 10 --smith-waterman FASTEST_AVAILABLE --emit-ref-confidence NONE --max-mnp-distance 1 --force-call-filtered-alleles false --reference-model-deletion-quality 30 --soft-clip-low-quality-ends false --allele-informative-reads-overlap-margin 2 --smith-waterman-dangling-end-match-value 25 --smith-waterman-dangling-end-mismatch-penalty -50 --smith-waterman-dangling-end-gap-open-penalty -110 --smith-waterman-dangling-end-gap-extend-penalty -6 --smith-waterman-haplotype-to-reference-match-value 200 --smith-waterman-haplotype-to-reference-mismatch-penalty -150 --smith-waterman-haplotype-to-reference-gap-open-penalty -260 --smith-waterman-haplotype-to-reference-gap-extend-penalty -11 --smith-waterman-read-to-haplotype-match-value 10 --smith-waterman-read-to-haplotype-mismatch-penalty -15 --smith-waterman-read-to-haplotype-gap-open-penalty -30 --smith-waterman-read-to-haplotype-gap-extend-penalty -5 --flow-assembly-collapse-hmer-size 0 --flow-assembly-collapse-partial-mode false --flow-filter-alleles false --flow-filter-alleles-qual-threshold 30.0 --flow-filter-alleles-sor-threshold 3.0 --flow-filter-lone-alleles false --flow-filter-alleles-debug-graphs false --min-assembly-region-size 50 --max-assembly-region-size 300 --active-probability-threshold 0.002 --max-prob-propagation-distance 50 --force-active false --assembly-region-padding 100 --padding-around-indels 75 --padding-around-snps 20 --padding-around-strs 75 --max-extension-into-assembly-region-padding-legacy 25 --max-reads-per-alignment-start 50 --enable-legacy-assembly-region-trimming false --interval-set-rule UNION --interval-padding 0 --interval-exclusion-padding 0 --interval-merging-rule ALL --read-validation-stringency SILENT --seconds-between-progress-updates 10.0 --disable-sequence-dictionary-validation false --create-output-bam-index true --create-output-bam-md5 false --create-output-variant-index true --create-output-variant-md5 false --max-variants-per-shard 0 --lenient false --add-output-sam-program-record true --add-output-vcf-command-line true --cloud-prefetch-buffer 40 --cloud-index-prefetch-buffer -1 --disable-bam-index-caching false --sites-only-vcf-output false --help false --version false --showHidden false --verbosity INFO --use-jdk-deflater false --use-jdk-inflater false --gcs-max-retries 20 --gcs-project-for-requester-pays  --disable-tool-default-read-filters false --max-read-length 2147483647 --min-read-length 30 --minimum-mapping-quality 20 --disable-tool-default-annotations false --enable-all-annotations false",Version="4.6.2.0",Date="February 2, 2026, 12:29:00?AM GMT">
##INFO=<ID=AS_SB_TABLE,Number=1,Type=String,Description="Allele-specific forward/reverse read counts for strand bias tests. Includes the reference and alleles separated by |.">
##INFO=<ID=AS_UNIQ_ALT_READ_COUNT,Number=A,Type=Integer,Description="Number of reads with unique start and mate end positions for each alt at a variant site">
##INFO=<ID=CONTQ,Number=1,Type=Float,Description="Phred-scaled qualities that alt allele are not due to contamination">
##INFO=<ID=DP,Number=1,Type=Integer,Description="Approximate read depth; some reads may have been filtered">
##INFO=<ID=ECNT,Number=1,Type=Integer,Description="Number of potential somatic events in the assembly region">
##INFO=<ID=ECNTH,Number=A,Type=Integer,Description="Number of somatic events in best supporting haplotype for each alt allele">
##INFO=<ID=GERMQ,Number=1,Type=Integer,Description="Phred-scaled quality that alt alleles are not germline variants">
##INFO=<ID=MBQ,Number=R,Type=Integer,Description="median base quality by allele">
##INFO=<ID=MFRL,Number=R,Type=Integer,Description="median fragment length by allele">
##INFO=<ID=MMQ,Number=R,Type=Integer,Description="median mapping quality by allele">
##INFO=<ID=MPOS,Number=A,Type=Integer,Description="median distance from end of read">
##INFO=<ID=NALOD,Number=A,Type=Float,Description="Log 10 odds of artifact in normal with same allele fraction as tumor">
##INFO=<ID=NCount,Number=1,Type=Integer,Description="Count of N bases in the pileup">
##INFO=<ID=NLOD,Number=A,Type=Float,Description="Normal log 10 likelihood ratio of diploid het or hom alt genotypes">
##INFO=<ID=OCM,Number=1,Type=Integer,Description="Number of alt reads whose original alignment doesn't match the current contig.">
##INFO=<ID=PON,Number=0,Type=Flag,Description="site found in panel of normals">
##INFO=<ID=POPAF,Number=A,Type=Float,Description="negative log 10 population allele frequencies of alt alleles">
##INFO=<ID=ROQ,Number=1,Type=Float,Description="Phred-scaled qualities that alt allele are not due to read orientation artifact">
##INFO=<ID=RPA,Number=R,Type=Integer,Description="Number of times tandem repeat unit is repeated, for each allele (including reference)">
##INFO=<ID=RU,Number=1,Type=String,Description="Tandem repeat unit (bases)">
##INFO=<ID=SEQQ,Number=1,Type=Integer,Description="Phred-scaled quality that alt alleles are not sequencing errors">
##INFO=<ID=STR,Number=0,Type=Flag,Description="Variant is a short tandem repeat">
##INFO=<ID=STRANDQ,Number=1,Type=Integer,Description="Phred-scaled quality of strand bias artifact">
##INFO=<ID=STRQ,Number=1,Type=Integer,Description="Phred-scaled quality that alt alleles in STRs are not polymerase slippage errors">
##INFO=<ID=TLOD,Number=A,Type=Float,Description="Log 10 likelihood ratio score of variant existing versus not existing">
##MutectVersion=2.2
##contig=<ID=K03455,length=9719>
##filtering_status=Warning: unfiltered Mutect 2 calls.  Please run FilterMutectCalls to remove false positives.
##source=Mutect2
##tumor_sample=SRR8525881
#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	SRR8525881
K03455	4098	.	T	C	.	.	AS_SB_TABLE=0,0|0,0;DP=2;ECNT=5;ECNTH=2;MBQ=0,34;MFRL=0,222;MMQ=60,60;MPOS=3;POPAF=7.30;TLOD=7.14	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:0,2:0.750:2:0,1:0,1:0,2:0,0,2,0
K03455	4139	.	G	A	.	.	AS_SB_TABLE=13,1|1,1;DP=16;ECNT=5;ECNTH=3;MBQ=37,27;MFRL=321,321;MMQ=60,60;MPOS=18;POPAF=7.30;TLOD=3.04	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:14,2:0.175:16:4,0:8,2:13,2:13,1,1,1
K03455	4145	.	T	C	.	.	AS_SB_TABLE=4,0|11,2;DP=17;ECNT=5;ECNTH=2;MBQ=39,33;MFRL=370,326;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=40.51	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:4,13:0.718:17:2,3:2,7:4,12:4,0,11,2
K03455	4148	.	G	A	.	.	AS_SB_TABLE=9,2|6,0;DP=17;ECNT=5;ECNTH=3;MBQ=33,38;MFRL=316,338;MMQ=60,60;MPOS=33;POPAF=7.30;TLOD=21.00	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:11,6:0.393:17:3,2:6,4:10,6:9,2,6,0
K03455	4166	.	C	T	.	.	AS_SB_TABLE=18,2|3,0;DP=23;ECNT=5;ECNTH=3;MBQ=35,37;MFRL=321,447;MMQ=60,60;MPOS=41;POPAF=7.30;TLOD=6.40	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:20,3:0.167:23:2,1:11,2:19,3:18,2,3,0
K03455	4190	.	A	G	.	.	AS_SB_TABLE=5,0|10,2;DP=17;ECNT=23;ECNTH=9;MBQ=38,38;MFRL=293,295;MMQ=60,60;MPOS=36;POPAF=7.30;TLOD=39.32	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:5,12:0.649:17:3,4:2,6:5,10:5,0,10,2
K03455	4209	.	GC	AA,AC	.	.	AS_SB_TABLE=0,0|12,2|4,0;DP=18;ECNT=23;ECNTH=8,14;MBQ=0,37,38;MFRL=0,288,310;MMQ=60,60,60;MPOS=41,55;POPAF=7.30,7.30;TLOD=57.24,11.52	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1/2:0,14,4:0.685,0.262:18:0,6,2:0,6,2:0,12,4:0,0,16,2
K03455	4233	.	T	C	.	.	AS_SB_TABLE=14,3|4,0;DP=21;ECNT=23;ECNTH=14;MBQ=38,35;MFRL=286,310;MMQ=60,60;MPOS=43;POPAF=7.30;TLOD=12.39	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:17,4:0.238:21:7,2:7,2:15,4:14,3,4,0
K03455	4259	.	T	A	.	.	AS_SB_TABLE=4,0|15,5;DP=25;ECNT=23;ECNTH=8;MBQ=33,37;MFRL=294,271;MMQ=60,60;MPOS=40;POPAF=7.30;TLOD=83.81	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:4,20:0.792:24:2,10:2,7:4,18:4,0,15,5
K03455	4262	.	A	T	.	.	AS_SB_TABLE=15,5|4,0;DP=25;ECNT=23;ECNTH=11;MBQ=28,35;MFRL=271,294;MMQ=60,60;MPOS=14;POPAF=7.30;TLOD=12.77	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:20,4:0.208:24:9,1:6,2:18,4:0|1:4262_A_T:4262:15,5,4,0
K03455	4268	.	G	A	.	.	AS_SB_TABLE=15,5|4,0;DP=24;ECNT=23;ECNTH=9;MBQ=34,28;MFRL=271,294;MMQ=60,60;MPOS=13;POPAF=7.30;TLOD=12.77	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:20,4:0.208:24:9,0:7,2:18,4:15,5,4,0
K03455	4277	.	C	T	.	.	AS_SB_TABLE=4,1|15,5;DP=25;ECNT=23;ECNTH=8;MBQ=20,33;MFRL=247,271;MMQ=60,60;MPOS=32;POPAF=7.30;TLOD=83.81	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	1|0:5,20:0.792:25:1,10:1,7:4,18:1|0:4262_A_T:4262:4,1,15,5
K03455	4279	.	GT	AC,AT	.	.	AS_SB_TABLE=4,1|12,3|3,2;DP=25;ECNT=23;ECNTH=8,8;MBQ=20,37,20;MFRL=247,290,184;MMQ=60,60,60;MPOS=34,33;POPAF=7.30,7.30;TLOD=47.62,10.92	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1/2:5,15,5:0.601,0.199:25:1,8,2:2,5,2:4,14,4:4,1,15,5
K03455	4310	.	C	T	.	.	AS_SB_TABLE=14,9|3,3;DP=29;ECNT=23;ECNTH=14;MBQ=20,20;MFRL=206,251;MMQ=60,60;MPOS=27;POPAF=7.30;TLOD=15.61	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:23,6:0.214:29:10,0:4,3:21,5:14,9,3,3
K03455	4320	.	G	A	.	.	AS_SB_TABLE=12,10|3,4;DP=29;ECNT=23;ECNTH=14;MBQ=20,20;MFRL=227,254;MMQ=60,60;MPOS=31;POPAF=7.30;TLOD=29.08	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:22,7:0.242:29:9,0:6,4:21,6:12,10,3,4
K03455	4334	.	A	G	.	.	AS_SB_TABLE=16,12|1,4;DP=33;ECNT=23;ECNTH=14;MBQ=20,33;MFRL=250,254;MMQ=60,60;MPOS=15;POPAF=7.30;TLOD=15.63	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:28,5:0.194:33:9,2:11,3:24,5:16,12,1,4
K03455	4340	.	A	G	.	.	AS_SB_TABLE=6,6|11,12;DP=35;ECNT=23;ECNTH=8;MBQ=35,20;MFRL=225,252;MMQ=60,60;MPOS=40;POPAF=7.30;TLOD=100.52	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:12,23:0.656:35:3,9:7,7:10,20:0|1:4340_A_G:4340:6,6,11,12
K03455	4343	.	C	A	.	.	AS_SB_TABLE=6,6|10,13;DP=35;ECNT=23;ECNTH=8;MBQ=33,20;MFRL=225,252;MMQ=60,60;MPOS=43;POPAF=7.30;TLOD=104.83	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:12,23:0.667:35:3,8:5,6:10,21:0|1:4340_A_G:4340:6,6,10,13
K03455	4345	.	GC	AT	.	.	AS_SB_TABLE=12,15|6,6;DP=39;ECNT=23;ECNTH=14;MBQ=20,37;MFRL=197,225;MMQ=60,60;MPOS=27;POPAF=7.30;TLOD=49.00	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	1|0:27,12:0.314:39:6,3:9,6:23,10:1|0:4340_A_G:4340:12,15,6,6
K03455	4358	.	T	C	.	.	AS_SB_TABLE=15,17|2,5;DP=40;ECNT=23;ECNTH=14;MBQ=20,35;MFRL=200,254;MMQ=60,60;MPOS=29;POPAF=7.30;TLOD=18.77	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:32,7:0.200:39:7,3:12,3:27,6:15,17,2,5
K03455	4361	.	GCT	ACT,G	.	.	AS_SB_TABLE=1,2|11,14|7,7;DP=42;ECNT=23;ECNTH=8,14;MBQ=18,20,34;MFRL=106,209,226;MMQ=60,60,60;MPOS=33,37;POPAF=7.30,7.30;TLOD=101.07,53.78	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1/2:3,25,14:0.615,0.308:42:0,5,4:0,9,7:2,23,11:1,2,18,21
K03455	4364	.	A	AAG	.	.	AS_SB_TABLE=12,16|8,7;DP=44;ECNT=23;ECNTH=14;MBQ=33,31;MFRL=206,205;MMQ=60,60;MPOS=38;POPAF=7.30;TLOD=56.51	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:28,15:0.333:43:7,4:12,7:25,12:12,16,8,7
K03455	4370	.	A	G	.	.	AS_SB_TABLE=11,13|7,13;DP=46;ECNT=23;ECNTH=8;MBQ=20,37;MFRL=191,291;MMQ=60,60;MPOS=36;POPAF=7.30;TLOD=64.83	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:24,20:0.538:44:6,5:9,9:17,20:11,13,7,13
K03455	4376	.	C	T	.	.	AS_SB_TABLE=9,17|10,12;DP=49;ECNT=23;ECNTH=14;MBQ=37,20;MFRL=267,204;MMQ=60,60;MPOS=31;POPAF=7.30;TLOD=76.44	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:26,22:0.404:48:4,6:11,9:24,16:9,17,10,12
K03455	4397	.	T	C	.	.	AS_SB_TABLE=17,18|13,9;DP=62;ECNT=23;ECNTH=14;MBQ=32,33;MFRL=290,247;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=79.03	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:35,22:0.386:57:5,8:14,10:31,19:17,18,13,9
K03455	4416	.	C	T	.	.	AS_SB_TABLE=16,21|2,1;DP=40;ECNT=19;ECNTH=7;MBQ=37,20;MFRL=281,287;MMQ=60,60;MPOS=9;POPAF=7.30;TLOD=8.03	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:37,3:0.103:40:13,0:18,1:34,3:16,21,2,1
K03455	4421	.	T	C	.	.	AS_SB_TABLE=3,5|15,17;DP=40;ECNT=19;ECNTH=9;MBQ=34,38;MFRL=218,289;MMQ=60,60;MPOS=32;POPAF=7.30;TLOD=135.40	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:8,32:0.802:40:4,11:1,16:7,30:3,5,15,17
K03455	4424	.	T	C	.	.	AS_SB_TABLE=4,7|14,15;DP=40;ECNT=19;ECNTH=9;MBQ=20,38;MFRL=206,291;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=111.14	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:11,29:0.748:40:4,8:1,16:9,28:4,7,14,15
K03455	4439	.	A	G	.	.	AS_SB_TABLE=15,17|1,2;DP=35;ECNT=19;ECNTH=6;MBQ=38,20;MFRL=291,126;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=8.08	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:32,3:0.088:35:11,1:16,1:30,2:15,17,1,2
K03455	4442	.	AG	GA	.	.	AS_SB_TABLE=13,15|3,4;DP=35;ECNT=19;ECNTH=6;MBQ=39,33;MFRL=297,209;MMQ=60,60;MPOS=27;POPAF=7.30;TLOD=21.88	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:28,7:0.235:35:11,1:13,5:25,7:13,15,3,4
K03455	4443	.	G	A	.	.	AS_SB_TABLE=3,5|11,11;DP=35;ECNT=19;ECNTH=9;MBQ=38,38;MFRL=258,297;MMQ=60,60;MPOS=38;POPAF=7.30;TLOD=80.00	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:8,22:0.726:30:3,9:3,8:7,20:3,5,11,11
K03455	4448	.	C	A	.	.	AS_SB_TABLE=14,17|1,2;DP=34;ECNT=19;ECNTH=9;MBQ=38,20;MFRL=290,126;MMQ=60,60;MPOS=25;POPAF=7.30;TLOD=9.60	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:31,3:0.091:34:10,0:16,1:29,2:14,17,1,2
K03455	4449	.	C	T	.	.	AS_SB_TABLE=6,7|9,10;DP=32;ECNT=19;ECNTH=9;MBQ=31,39;MFRL=206,323;MMQ=60,60;MPOS=42;POPAF=7.30;TLOD=74.99	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:13,19:0.617:32:4,7:6,10:11,18:6,7,9,10
K03455	4460	.	T	C	.	.	AS_SB_TABLE=15,14|0,2;DP=31;ECNT=19;ECNTH=7;MBQ=37,38;MFRL=290,312;MMQ=60,60;MPOS=40;POPAF=7.30;TLOD=4.96	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:29,2:0.100:31:9,0:14,2:26,2:15,14,0,2
K03455	4465	.	T	TGGCC	.	.	AS_SB_TABLE=13,12|2,3;DP=30;ECNT=19;ECNTH=8;MBQ=37,28;MFRL=290,229;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=13.23	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:25,5:0.148:30:8,2:14,1:23,4:0|1:4465_T_TGGCC:4465:13,12,2,3
K03455	4466	.	A	AGTG	.	.	AS_SB_TABLE=12,12|2,3;DP=30;ECNT=19;ECNTH=8;MBQ=38,29;MFRL=307,229;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=13.49	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:24,5:0.153:29:7,2:13,1:22,4:0|1:4465_T_TGGCC:4465:12,12,2,3
K03455	4478	.	T	C	.	.	AS_SB_TABLE=11,9|3,2;DP=25;ECNT=19;ECNTH=8;MBQ=38,20;MFRL=323,207;MMQ=60,60;MPOS=57;POPAF=7.30;TLOD=17.41	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:20,5:0.200:25:6,3:13,1:19,4:11,9,3,2
K03455	4505	.	A	G	.	.	AS_SB_TABLE=2,1|10,4;DP=17;ECNT=19;ECNTH=9;MBQ=39,38;MFRL=394,329;MMQ=60,60;MPOS=38;POPAF=7.30;TLOD=58.83	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:3,14:0.789:17:2,5:1,8:3,14:2,1,10,4
K03455	4508	.	A	T	.	.	AS_SB_TABLE=2,2|10,3;DP=17;ECNT=19;ECNTH=9;MBQ=39,35;MFRL=341,335;MMQ=60,60;MPOS=31;POPAF=7.30;TLOD=53.87	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:4,13:0.737:17:2,5:2,8:4,13:2,2,10,3
K03455	4511	.	G	A	.	.	AS_SB_TABLE=2,1|10,4;DP=17;ECNT=19;ECNTH=9;MBQ=37,36;MFRL=394,329;MMQ=60,60;MPOS=32;POPAF=7.30;TLOD=58.91	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:3,14:0.789:17:2,4:1,7:3,14:2,1,10,4
K03455	4526	.	T	C	.	.	AS_SB_TABLE=0,0|11,2;DP=13;ECNT=19;ECNTH=9;MBQ=0,36;MFRL=0,323;MMQ=60,60;MPOS=28;POPAF=7.30;TLOD=56.96	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:0,13:0.933:13:0,7:0,6:0,13:0,0,11,2
K03455	4528	.	T	TCA	.	.	AS_SB_TABLE=10,1|1,1;DP=13;ECNT=19;ECNTH=7;MBQ=38,37;MFRL=323,421;MMQ=60,60;MPOS=38;POPAF=7.30;TLOD=5.76	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:11,2:0.200:13:5,1:4,1:11,2:0|1:4528_T_TCA:4528:10,1,1,1
K03455	4530	.	CTT	C	.	.	AS_SB_TABLE=9,1|1,1;DP=13;ECNT=19;ECNTH=7;MBQ=35,38;MFRL=323,421;MMQ=60,60;MPOS=34;POPAF=7.30;RPA=4,2;RU=T;STR;TLOD=6.12	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:10,2:0.213:12:5,1:4,1:10,2:0|1:4528_T_TCA:4528:9,1,1,1
K03455	4532	.	T	C	.	.	AS_SB_TABLE=0,0|11,0;DP=13;ECNT=19;ECNTH=9;MBQ=0,27;MFRL=0,323;MMQ=60,60;MPOS=21;POPAF=7.30;TLOD=39.42	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:0,11:0.926:11:0,6:0,2:0,11:0,0,11,0