Mercurial > repos > iuc > disco
annotate disco.xml @ 3:b6d2f5be3f88 draft default tip
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
| author | iuc |
|---|---|
| date | Tue, 31 Aug 2021 08:02:33 +0000 |
| parents | 56b0772e3379 |
| children |
| rev | line source |
|---|---|
|
2
56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
0
diff
changeset
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1 <tool id="disco" name="DISCO" version="@WRAPPER_VERSION@.1" profile="20.01"> |
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0
3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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2 <description>to assemble metagenomics data using an overlap-layout-consensus (OLC) approach</description> |
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3
b6d2f5be3f88
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
iuc
parents:
2
diff
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3 <xrefs> |
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b6d2f5be3f88
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
iuc
parents:
2
diff
changeset
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4 <xref type="bio.tools">disco</xref> |
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b6d2f5be3f88
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
iuc
parents:
2
diff
changeset
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5 </xrefs> |
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0
3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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6 <macros> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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7 <token name="@WRAPPER_VERSION@">1.2</token> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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8 <xml name="assembly_params"> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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9 <param argument="minSequenceLengthTobePrinted" type="integer" value="1000" min="0" label="Minimum length of contigs or scaffolds to be printed"/> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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10 <param argument="MinOverlap4SimplifyGraph" type="integer" value="30" min="0" label="Minimum overlap length (bp) required to keep an edge between two reads during graph simplification" help="This minimum overlap length must be equal to 30 or larger than the MinOverlap4BuildGraph above. This allows you to try different minimum overlap lengths for assembly without redoing assembly graph construction. Edges with shorter overlap length than this parameter will be ignored during graph simplification. Increase this to reduce N50 and mis-assemblies"/> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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11 <param argument="minOverlapDifference4ClipBranches" type="integer" value="25" min="0" label="Minimum overlap length difference (bp) to clip branches" help="If a read has multiple edges, Disco clips the branches with overlap lengths less than the largest overlap of this read by this difference or more. Increase this to reduce N50 and mis-assemblies"/> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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12 <param argument="minUniquePEsupport" type="integer" value="3" min="0" label="Minumum number of paired-end reads that provide unique support to merge two edges" help="Increase this to reduce N50 and mis-assemblies"/> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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13 <param argument="minNonUniquePEsupport" type="integer" value="0" min="0" label="Minumum number of paired-end reads that provide non-unique support to merge two edgess"/> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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14 <param argument="minReadsCountInEdgeToBeNotDeadEnd" type="integer" value="10" min="0" label="Minimum number of reads in an edge to be not dead-end edge"/> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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15 <param argument="minEdgeLengthToBeNotDeadEnd" type="integer" value="1000" min="0" label="Minimum edge length (bp) to be not dead-end edge"/> |
|
3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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16 <param argument="minFoldToBeShortBranch" type="integer" value="5" min="0" label="Minimum fold difference between two branches' lengths to consider a branch to be short"/> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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17 <param argument="minReadsCountToHave0Flow" type="integer" value="15" min="0" label="Minimum number of reads for an edge to be kept even if it has 0 flow"/> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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18 <param argument="minEdgeLengthToHave0Flow" type="integer" value="1500" min="0" label="Minimum edge length for an edge to be kept even if it has 0 flow"/> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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19 <param argument="minReadsCountInEdgeToBe1MinFlow" type="integer" value="20" min="0" label="Minimum number of reads in an edge to be assigned with 1 minimum flow"/> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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20 <param argument="minEdgeLengthToBe1MinFlow" type="integer" value="2000" min="0" label="Minimum edge length to be assigned with 1 minimum flow"/> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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21 </xml> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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22 </macros> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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23 <requirements> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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24 <requirement type="package" version="@WRAPPER_VERSION@">disco</requirement> |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
changeset
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25 </requirements> |
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2
56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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26 <version_command>echo @WRAPPER_VERSION@</version_command> |
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0
3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
parents:
diff
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27 <command detect_errors="exit_code"><![CDATA[ |
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2
56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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28 #if $input_cond.library_type == "single" |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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29 #for i, e in enumerate($input_cond.inS) |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
0
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30 cp '$e' |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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31 #if $e.ext.endswith(".gz") |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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32 input_${i}.fastq.gz && |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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33 #else |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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34 input_${i}.fastq && |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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35 #end if |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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36 #end for |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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37 #else |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
0
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38 #if $input_cond.paired_input.type == "separated" |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
0
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39 #if $input_cond.paired_input.in1.ext.endswith(".gz") |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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40 cp '$input_cond.paired_input.in1' forward.fastq.gz && |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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41 cp '$input_cond.paired_input.in2' reverse.fastq.gz && |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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42 #else |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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43 cp '$input_cond.paired_input.in1' forward.fastq && |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
0
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44 cp '$input_cond.paired_input.in2' reverse.fastq && |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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45 #end if |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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46 #else |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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47 #for i, e in enumerate($input_cond.paired_input.inP) |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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48 cp '$e' |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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49 #if $e.ext.endswith(".gz") |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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50 input_${i}.fastq.gz && |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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51 #else |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
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52 input_${i}.fastq && |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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53 #end if |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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54 #end for |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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55 #end if |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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56 #end if |
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0
3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
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57 runDisco.sh |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
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58 -n \${GALAXY_SLOTS:-4} |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
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59 -m \${DISCO_MAX_MEMORY:-4} |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
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60 -d . |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
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61 -o disco |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
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62 #if $input_cond.library_type == "single" |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
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63 -inS \$(find . -name "*fastq*" -print | tr '\n' ',' | sed 's/,$//') |
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3c16cb5b2b5b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit c1327b5793477da611684e92e0ce4635370c7666
iuc
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64 #else |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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65 #if $input_cond.paired_input.type == "separated" |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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66 #if $input_cond.paired_input.in1.ext.endswith(".gz") |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
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67 -in1 forward.fastq.gz |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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68 -in2 reverse.fastq.gz |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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69 #else |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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70 -in1 forward.fastq |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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71 -in2 reverse.fastq |
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56b0772e3379
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/disco/ commit d9fc31caa31f1f0c63a0dd5ffe136d765d607080"
iuc
parents:
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72 #end if |
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73 #else |
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2
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74 -inP \$(find . -name "*fastq*" -print | tr '\n' ',' | sed 's/,$//') |
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75 #end if |
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76 #end if |
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77 $obg |
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78 $osg |
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79 -p '$first_assembly_iteration_params' |
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80 -p2 '$second_assembly_iteration_params' |
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81 -p3 '$third_assembly_iteration_params' |
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82 ]]></command> |
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83 <configfiles> |
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84 <configfile name="first_assembly_iteration_params"> |
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85 <![CDATA[ |
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86 MinOverlap4BuildGraph = $MinOverlap4BuildGraph |
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87 PrintContigs = true |
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88 PrintScaffolds = true |
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89 PrintUnused = $PrintUnused |
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90 PrintGFA = $PrintGFA |
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91 PrintGFA2 = $PrintGFA2 |
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92 minSequenceLengthTobePrinted = $first_assembly_iter_param.minSequenceLengthTobePrinted |
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93 MinOverlap4SimplifyGraph = $first_assembly_iter_param.MinOverlap4SimplifyGraph |
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94 minOverlapDifference4ClipBranches = $first_assembly_iter_param.minOverlapDifference4ClipBranches |
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95 minUniquePEsupport = $first_assembly_iter_param.minUniquePEsupport |
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96 minNonUniquePEsupport = $first_assembly_iter_param.minNonUniquePEsupport |
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97 minReadsCountInEdgeToBeNotDeadEnd = $first_assembly_iter_param.minReadsCountInEdgeToBeNotDeadEnd |
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98 minEdgeLengthToBeNotDeadEnd = $first_assembly_iter_param.minEdgeLengthToBeNotDeadEnd |
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99 minFoldToBeShortBranch = $first_assembly_iter_param.minFoldToBeShortBranch |
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100 minReadsCountToHave0Flow = $first_assembly_iter_param.minReadsCountToHave0Flow |
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101 minEdgeLengthToHave0Flow = $first_assembly_iter_param.minEdgeLengthToHave0Flow |
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102 minReadsCountInEdgeToBe1MinFlow = $first_assembly_iter_param.minReadsCountInEdgeToBe1MinFlow |
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103 minEdgeLengthToBe1MinFlow = $first_assembly_iter_param.minEdgeLengthToBe1MinFlow |
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104 ]]></configfile> |
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105 <configfile name="second_assembly_iteration_params"> |
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106 <![CDATA[ |
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107 MinOverlap4BuildGraph = $MinOverlap4BuildGraph |
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108 PrintContigs = true |
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109 PrintScaffolds = true |
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110 PrintUnused = $PrintUnused |
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111 PrintGFA = $PrintGFA |
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112 PrintGFA2 = $PrintGFA2 |
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113 minSequenceLengthTobePrinted = $second_assembly_iter_param.minSequenceLengthTobePrinted |
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114 MinOverlap4SimplifyGraph = $second_assembly_iter_param.MinOverlap4SimplifyGraph |
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115 minOverlapDifference4ClipBranches = $second_assembly_iter_param.minOverlapDifference4ClipBranches |
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116 minUniquePEsupport = $second_assembly_iter_param.minUniquePEsupport |
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117 minNonUniquePEsupport = $second_assembly_iter_param.minNonUniquePEsupport |
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118 minReadsCountInEdgeToBeNotDeadEnd = $second_assembly_iter_param.minReadsCountInEdgeToBeNotDeadEnd |
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119 minEdgeLengthToBeNotDeadEnd = $second_assembly_iter_param.minEdgeLengthToBeNotDeadEnd |
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120 minFoldToBeShortBranch = $second_assembly_iter_param.minFoldToBeShortBranch |
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121 minReadsCountToHave0Flow = $second_assembly_iter_param.minReadsCountToHave0Flow |
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122 minEdgeLengthToHave0Flow = $second_assembly_iter_param.minEdgeLengthToHave0Flow |
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123 minReadsCountInEdgeToBe1MinFlow = $second_assembly_iter_param.minReadsCountInEdgeToBe1MinFlow |
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124 minEdgeLengthToBe1MinFlow = $second_assembly_iter_param.minEdgeLengthToBe1MinFlow |
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125 ]]></configfile> |
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126 <configfile name="third_assembly_iteration_params"> |
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127 <![CDATA[ |
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128 MinOverlap4BuildGraph = $MinOverlap4BuildGraph |
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129 PrintContigs = true |
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130 PrintScaffolds = true |
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131 PrintUnused = $PrintUnused |
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132 PrintGFA = $PrintGFA |
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133 PrintGFA2 = $PrintGFA2 |
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134 minSequenceLengthTobePrinted = $third_assembly_iter_param.minSequenceLengthTobePrinted |
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135 MinOverlap4SimplifyGraph = $third_assembly_iter_param.MinOverlap4SimplifyGraph |
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136 minOverlapDifference4ClipBranches = $third_assembly_iter_param.minOverlapDifference4ClipBranches |
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137 minUniquePEsupport = $third_assembly_iter_param.minUniquePEsupport |
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138 minNonUniquePEsupport = $third_assembly_iter_param.minNonUniquePEsupport |
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139 minReadsCountInEdgeToBeNotDeadEnd = $third_assembly_iter_param.minReadsCountInEdgeToBeNotDeadEnd |
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140 minEdgeLengthToBeNotDeadEnd = $third_assembly_iter_param.minEdgeLengthToBeNotDeadEnd |
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141 minFoldToBeShortBranch = $third_assembly_iter_param.minFoldToBeShortBranch |
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142 minReadsCountToHave0Flow = $third_assembly_iter_param.minReadsCountToHave0Flow |
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143 minEdgeLengthToHave0Flow = $third_assembly_iter_param.minEdgeLengthToHave0Flow |
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144 minReadsCountInEdgeToBe1MinFlow = $third_assembly_iter_param.minReadsCountInEdgeToBe1MinFlow |
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145 minEdgeLengthToBe1MinFlow = $third_assembly_iter_param.minEdgeLengthToBe1MinFlow |
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146 ]]></configfile> |
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147 </configfiles> |
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148 <inputs> |
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149 <conditional name="input_cond"> |
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150 <param name="library_type" type="select" label="Type of library?"> |
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151 <option value="single">Single-end reads</option> |
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152 <option value="paired">Paired-end reads</option> |
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153 </param> |
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154 <when value="single"> |
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155 <param argument="-inS" type="data" format="fastq,fastq.gz,fasta" multiple="true" label="Single-read files"/> |
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156 </when> |
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157 <when value="paired"> |
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158 <conditional name="paired_input"> |
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159 <param name="type" type="select" label="Type of library?"> |
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160 <option value="separated">Separated forward/reverse paired-read files</option> |
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161 <option value="interleaved">Interleaved paired-read files</option> |
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162 </param> |
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163 <when value="separated"> |
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164 <param argument="-in1" type="data" format="fastq,fastq.gz,fasta" label="Forward read file" /> |
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165 <param argument="-in2" type="data" format="fastq,fastq.gz,fasta" label="Reverse read file" /> |
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166 </when> |
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167 <when value="interleaved"> |
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168 <param argument="-inP" type="data" format="fastq,fastq.gz,fasta" multiple="true" label="Interleaved paired-read files" /> |
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169 </when> |
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170 </conditional> |
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171 </when> |
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172 </conditional> |
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173 <param argument="-obg" type="boolean" truevalue="-obg" falsevalue="" checked="false" label="Only build overlap graph?"/> |
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174 <param argument="-osg" type="boolean" truevalue="-osg" falsevalue="" checked="false" label="Only simplify existing overlap graph?"/> |
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175 <param argument="MinOverlap4BuildGraph" type="integer" min="0" value="20" label="Minimum overlap length (bp) required to insert an edge between two reads during graph construction" help="Increase this to reduce N50 and mis-assemblies"/> |
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176 <param name="log" type="boolean" truevalue="true" falsevalue="false" checked="false" label="Output log file?"/> |
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177 <param argument="PrintScaffolds" type="boolean" truevalue="true" falsevalue="false" label="Print scaffolds?"/> |
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178 <param argument="PrintUnused" type="boolean" truevalue="true" falsevalue="false" label="Print unused reads?"/> |
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179 <param argument="PrintGFA" type="boolean" truevalue="true" falsevalue="false" label="Print GFA graph format?"/> |
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180 <param argument="PrintGFA2" type="boolean" truevalue="true" falsevalue="false" label="Print GFA2 graph format?"/> |
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181 <section name="first_assembly_iter_param" title="Parameters for 1st assembly iteration" expanded="False"> |
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182 <expand macro="assembly_params"/> |
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183 </section> |
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184 <section name="second_assembly_iter_param" title="Parameters for 2nd assembly iteration" expanded="False"> |
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185 <expand macro="assembly_params"/> |
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186 </section> |
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187 <section name="third_assembly_iter_param" title="Parameters for 3rd assembly iteration" expanded="False"> |
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188 <expand macro="assembly_params"/> |
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189 </section> |
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190 </inputs> |
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191 <outputs> |
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192 <data format="txt" name="log" from_work_dir="disco.log" label="${tool.name} on ${on_string}: Log file"> |
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193 <filter>log</filter> |
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194 </data> |
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195 <data format="fasta" name="contigs" from_work_dir="disco_contigsFinalCombined.fasta" label="${tool.name} on ${on_string}: Contigs"/> |
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196 <data format="fasta" name="scaffolds" from_work_dir="disco_scaffoldsFinalCombined.fasta" label="${tool.name} on ${on_string}: Scaffolds"/> |
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197 <collection name="unused_reads" type="list" label="${tool.name} on ${on_string}: Unused reads"> |
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198 <filter>PrintUnused</filter> |
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199 <discover_datasets pattern="disco_0_Unused(?P<designation>.+)\.fasta" format="fasta" directory="assembly" /> |
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200 </collection> |
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201 <collection name="gfa_graphs" type="list" label="${tool.name} on ${on_string}: GFA graph"> |
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202 <filter>PrintGFA</filter> |
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203 <discover_datasets pattern="disco_Graph_(?P<designation>.+)\.gfa" format="txt" directory="assembly" /> |
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204 </collection> |
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205 <collection name="gfa2_graphs" type="list" label="${tool.name} on ${on_string}: GFA2 graph"> |
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206 <filter>PrintGFA2</filter> |
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207 <discover_datasets pattern="disco_Graph_(?P<designation>.+)\.gfa2" format="txt" directory="assembly" /> |
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208 </collection> |
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209 </outputs> |
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210 <tests> |
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211 <test> |
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212 <conditional name="input_cond"> |
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213 <param name="library_type" value="paired"/> |
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214 <conditional name="paired_input"> |
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215 <param name="type" value="separated"/> |
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216 <param name="in1" value="ecoli_1K_1.fq"/> |
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217 <param name="in2" value="ecoli_1K_2.fq"/> |
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218 </conditional> |
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219 </conditional> |
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220 <param name="obg" value=""/> |
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221 <param name="osg" value=""/> |
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222 <param name="MinOverlap4BuildGraph" value="20"/> |
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223 <param name="log" value="true"/> |
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224 <param name="PrintUnused" value="true"/> |
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225 <param name="PrintGFA" value="true"/> |
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226 <param name="PrintGFA2" value="true"/> |
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227 <section name="first_assembly_iter_param"> |
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228 <param name="minSequenceLengthTobePrinted" value="1000"/> |
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229 <param name="MinOverlap4SimplifyGraph" value="30"/> |
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230 <param name="minOverlapDifference4ClipBranches" value="25"/> |
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231 <param name="minUniquePEsupport" value="3"/> |
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232 <param name="minNonUniquePEsupport" value="0"/> |
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233 <param name="minReadsCountInEdgeToBeNotDeadEnd" value="10"/> |
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234 <param name="minEdgeLengthToBeNotDeadEnd" value="1000"/> |
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235 <param name="minFoldToBeShortBranch" value="5"/> |
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236 <param name="minReadsCountToHave0Flow" value="15"/> |
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237 <param name="minEdgeLengthToHave0Flow" value="1500"/> |
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238 <param name="minReadsCountInEdgeToBe1MinFlow" value="20"/> |
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239 <param name="minEdgeLengthToBe1MinFlow" value="2000"/> |
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240 </section> |
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241 <section name="second_assembly_iter_param"> |
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242 <param name="minSequenceLengthTobePrinted" value="1000"/> |
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243 <param name="MinOverlap4SimplifyGraph" value="30"/> |
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244 <param name="minOverlapDifference4ClipBranches" value="25"/> |
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245 <param name="minUniquePEsupport" value="3"/> |
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246 <param name="minNonUniquePEsupport" value="0"/> |
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247 <param name="minReadsCountInEdgeToBeNotDeadEnd" value="10"/> |
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248 <param name="minEdgeLengthToBeNotDeadEnd" value="1000"/> |
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249 <param name="minFoldToBeShortBranch" value="5"/> |
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250 <param name="minReadsCountToHave0Flow" value="15"/> |
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251 <param name="minEdgeLengthToHave0Flow" value="1500"/> |
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252 <param name="minReadsCountInEdgeToBe1MinFlow" value="20"/> |
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253 <param name="minEdgeLengthToBe1MinFlow" value="2000"/> |
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254 </section> |
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255 <section name="third_assembly_iter_param"> |
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256 <param name="minSequenceLengthTobePrinted" value="1000"/> |
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257 <param name="MinOverlap4SimplifyGraph" value="30"/> |
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258 <param name="minOverlapDifference4ClipBranches" value="25"/> |
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259 <param name="minUniquePEsupport" value="3"/> |
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260 <param name="minNonUniquePEsupport" value="0"/> |
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261 <param name="minReadsCountInEdgeToBeNotDeadEnd" value="10"/> |
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262 <param name="minEdgeLengthToBeNotDeadEnd" value="1000"/> |
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263 <param name="minFoldToBeShortBranch" value="5"/> |
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264 <param name="minReadsCountToHave0Flow" value="15"/> |
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265 <param name="minEdgeLengthToHave0Flow" value="1500"/> |
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266 <param name="minReadsCountInEdgeToBe1MinFlow" value="20"/> |
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267 <param name="minEdgeLengthToBe1MinFlow" value="2000"/> |
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268 </section> |
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269 <output name="log"> |
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270 <assert_contents> |
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271 <has_text text="Software: Disco Assembler" /> |
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272 <has_text text="FIRST LOOP ITERATION 1" /> |
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273 <has_text text="2054 reads streamed from this read file" /> |
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274 <has_text text="Graph simplification" /> |
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275 </assert_contents> |
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276 </output> |
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277 <output name="contigs" value="test1_contigs.fasta" compare="sim_size" delta="5"/> |
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278 <output name="scaffolds" value="test1_scaffolds.fasta" compare="sim_size" delta="5"/> |
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279 <output_collection name="unused_reads" type="list"> |
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280 <element name="PairedReads1"> |
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281 <assert_contents> |
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282 <has_text text="EAS20_8_6_1_9_1972/1" /> |
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283 <has_text text="EAS20_8_6_10_415_709/1" /> |
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284 <has_text text="EAS20_8_6_36_1124_470/1" /> |
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285 <has_text text="EAS20_8_6_100_1637_1332/1" /> |
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286 </assert_contents> |
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287 </element> |
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288 <element name="PairedReads2"> |
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289 <assert_contents> |
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290 <has_text text="EAS20_8_6_1_9_1972/2" /> |
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291 <has_text text="EAS20_8_6_10_415_709/2" /> |
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292 <has_text text="EAS20_8_6_36_1124_470/2" /> |
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293 <has_text text="EAS20_8_6_100_1637_1332/2" /> |
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294 </assert_contents> |
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295 </element> |
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296 </output_collection> |
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297 <output_collection name="gfa_graphs" type="list"> |
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298 <element name="1" file="test1_graph_1.gfa" compare="sim_size" delta="6000"/> |
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299 </output_collection> |
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300 <output_collection name="gfa2_graphs" type="list"> |
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301 <element name="1" file="test1_graph_1.gfa2" compare="sim_size" delta="6000"/> |
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302 </output_collection> |
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303 </test> |
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304 <test> |
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305 <conditional name="input_cond"> |
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306 <param name="library_type" value="paired"/> |
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307 <conditional name="paired_input"> |
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308 <param name="type" value="interleaved"/> |
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309 <param name="inP" value="ecoli_1K_interleaved.fq,ecoli_1K_interleaved.fq"/> |
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310 </conditional> |
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311 </conditional> |
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312 <param name="obg" value=""/> |
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313 <param name="osg" value=""/> |
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314 <param name="MinOverlap4BuildGraph" value="20"/> |
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315 <param name="log" value="true"/> |
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316 <param name="PrintUnused" value="false"/> |
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317 <param name="PrintGFA" value="false"/> |
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318 <param name="PrintGFA2" value="false"/> |
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319 <section name="first_assembly_iter_param"> |
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320 <param name="minSequenceLengthTobePrinted" value="1000"/> |
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321 <param name="MinOverlap4SimplifyGraph" value="30"/> |
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322 <param name="minOverlapDifference4ClipBranches" value="25"/> |
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323 <param name="minUniquePEsupport" value="3"/> |
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324 <param name="minNonUniquePEsupport" value="0"/> |
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325 <param name="minReadsCountInEdgeToBeNotDeadEnd" value="10"/> |
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326 <param name="minEdgeLengthToBeNotDeadEnd" value="1000"/> |
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327 <param name="minFoldToBeShortBranch" value="5"/> |
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328 <param name="minReadsCountToHave0Flow" value="15"/> |
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329 <param name="minEdgeLengthToHave0Flow" value="1500"/> |
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330 <param name="minReadsCountInEdgeToBe1MinFlow" value="20"/> |
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331 <param name="minEdgeLengthToBe1MinFlow" value="2000"/> |
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332 </section> |
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333 <section name="second_assembly_iter_param"> |
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334 <param name="minSequenceLengthTobePrinted" value="1000"/> |
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335 <param name="MinOverlap4SimplifyGraph" value="30"/> |
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336 <param name="minOverlapDifference4ClipBranches" value="25"/> |
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337 <param name="minUniquePEsupport" value="3"/> |
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338 <param name="minNonUniquePEsupport" value="0"/> |
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339 <param name="minReadsCountInEdgeToBeNotDeadEnd" value="10"/> |
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340 <param name="minEdgeLengthToBeNotDeadEnd" value="1000"/> |
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341 <param name="minFoldToBeShortBranch" value="5"/> |
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342 <param name="minReadsCountToHave0Flow" value="15"/> |
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343 <param name="minEdgeLengthToHave0Flow" value="1500"/> |
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344 <param name="minReadsCountInEdgeToBe1MinFlow" value="20"/> |
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345 <param name="minEdgeLengthToBe1MinFlow" value="2000"/> |
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346 </section> |
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347 <section name="third_assembly_iter_param"> |
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348 <param name="minSequenceLengthTobePrinted" value="1000"/> |
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349 <param name="MinOverlap4SimplifyGraph" value="30"/> |
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350 <param name="minOverlapDifference4ClipBranches" value="25"/> |
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351 <param name="minUniquePEsupport" value="3"/> |
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352 <param name="minNonUniquePEsupport" value="0"/> |
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353 <param name="minReadsCountInEdgeToBeNotDeadEnd" value="10"/> |
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354 <param name="minEdgeLengthToBeNotDeadEnd" value="1000"/> |
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355 <param name="minFoldToBeShortBranch" value="5"/> |
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3c16cb5b2b5b
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356 <param name="minReadsCountToHave0Flow" value="15"/> |
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357 <param name="minEdgeLengthToHave0Flow" value="1500"/> |
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358 <param name="minReadsCountInEdgeToBe1MinFlow" value="20"/> |
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359 <param name="minEdgeLengthToBe1MinFlow" value="2000"/> |
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360 </section> |
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361 <output name="log"> |
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362 <assert_contents> |
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363 <has_text text="Software: Disco Assembler" /> |
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364 <has_text text="FIRST LOOP ITERATION 1" /> |
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365 <has_text text="4108 reads streamed from this read file" /> |
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366 <has_text text="Graph simplification" /> |
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367 </assert_contents> |
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368 </output> |
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369 <output name="contigs" value="test2_contigs.fasta" compare="sim_size" delta="5"/> |
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370 <output name="scaffolds" value="test2_scaffolds.fasta" compare="sim_size" delta="5"/> |
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371 </test> |
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372 <test> |
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373 <conditional name="input_cond"> |
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374 <param name="library_type" value="single"/> |
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375 <param name="inS" value="ecoli_1K_1.fq.gz" ftype="fastq.gz"/> |
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376 </conditional> |
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377 <param name="obg" value=""/> |
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378 <param name="osg" value=""/> |
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379 <param name="MinOverlap4BuildGraph" value="20"/> |
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380 <param name="log" value="false"/> |
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381 <param name="PrintUnused" value="false"/> |
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382 <param name="PrintGFA" value="false"/> |
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383 <param name="PrintGFA2" value="false"/> |
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384 <section name="first_assembly_iter_param"> |
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385 <param name="minSequenceLengthTobePrinted" value="1000"/> |
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386 <param name="MinOverlap4SimplifyGraph" value="30"/> |
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387 <param name="minOverlapDifference4ClipBranches" value="25"/> |
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388 <param name="minUniquePEsupport" value="3"/> |
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389 <param name="minNonUniquePEsupport" value="0"/> |
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390 <param name="minReadsCountInEdgeToBeNotDeadEnd" value="10"/> |
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391 <param name="minEdgeLengthToBeNotDeadEnd" value="1000"/> |
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392 <param name="minFoldToBeShortBranch" value="5"/> |
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393 <param name="minReadsCountToHave0Flow" value="15"/> |
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394 <param name="minEdgeLengthToHave0Flow" value="1500"/> |
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395 <param name="minReadsCountInEdgeToBe1MinFlow" value="20"/> |
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396 <param name="minEdgeLengthToBe1MinFlow" value="2000"/> |
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397 </section> |
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398 <section name="second_assembly_iter_param"> |
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399 <param name="minSequenceLengthTobePrinted" value="1000"/> |
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400 <param name="MinOverlap4SimplifyGraph" value="30"/> |
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401 <param name="minOverlapDifference4ClipBranches" value="25"/> |
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402 <param name="minUniquePEsupport" value="3"/> |
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403 <param name="minNonUniquePEsupport" value="0"/> |
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404 <param name="minReadsCountInEdgeToBeNotDeadEnd" value="10"/> |
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405 <param name="minEdgeLengthToBeNotDeadEnd" value="1000"/> |
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406 <param name="minFoldToBeShortBranch" value="5"/> |
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407 <param name="minReadsCountToHave0Flow" value="15"/> |
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408 <param name="minEdgeLengthToHave0Flow" value="1500"/> |
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409 <param name="minReadsCountInEdgeToBe1MinFlow" value="20"/> |
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410 <param name="minEdgeLengthToBe1MinFlow" value="2000"/> |
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411 </section> |
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412 <section name="third_assembly_iter_param"> |
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413 <param name="minSequenceLengthTobePrinted" value="1000"/> |
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414 <param name="MinOverlap4SimplifyGraph" value="30"/> |
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415 <param name="minOverlapDifference4ClipBranches" value="25"/> |
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416 <param name="minUniquePEsupport" value="3"/> |
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417 <param name="minNonUniquePEsupport" value="0"/> |
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418 <param name="minReadsCountInEdgeToBeNotDeadEnd" value="10"/> |
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419 <param name="minEdgeLengthToBeNotDeadEnd" value="1000"/> |
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420 <param name="minFoldToBeShortBranch" value="5"/> |
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421 <param name="minReadsCountToHave0Flow" value="15"/> |
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422 <param name="minEdgeLengthToHave0Flow" value="1500"/> |
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423 <param name="minReadsCountInEdgeToBe1MinFlow" value="20"/> |
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424 <param name="minEdgeLengthToBe1MinFlow" value="2000"/> |
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425 </section> |
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426 <output name="contigs" value="test3_contigs.fasta" compare="sim_size" delta="5"/> |
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427 <output name="scaffolds" value="test3_scaffolds.fasta" compare="sim_size" delta="5"/> |
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428 </test> |
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429 </tests> |
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430 <help><![CDATA[ |
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431 DISCO is a multi threaded and multiprocess distributed memory overlap-layout-consensus (OLC) metagenome assembler. Disco was developed as a scalable assembler to assemble large metagenomes from billions of Illumina sequencing reads of complex microbial communities. Disco was parallelized for computer clusters in a hybrid architecture that integrated shared-memory multi-threading, point-to-point message passing, and remote direct memory access. The assembly and scaffolding were performed using an iterative overlap graph approach. |
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432 |
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433 The detailed user manual of the assembler and how to use it to acheive best results is provided here: |
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434 http://disco.omicsbio.org/user-manual. This is a quick start guide generally for developers and testers. Users with limited experience with genome assembly are advised to use the user manual. |
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435 ]]></help> |
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436 <citations> |
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437 </citations> |
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438 </tool> |
