Mercurial > repos > iuc > dexseq

annotate dexseq.xml @ 14:e5fe98b50c21 draft

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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dexseq commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
author iuc
date Tue, 31 Aug 2021 08:01:42 +0000
parents 577d1c8baab4
children 781f60a0c658
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1 <tool id="dexseq" name="DEXSeq" version="@VERSION@+galaxy1">
0
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2 <description>Determines differential exon usage from count tables</description>
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e5fe98b50c21 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dexseq commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
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3 <xrefs>
e5fe98b50c21 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dexseq commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
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4 <xref type="bio.tools">dexseq</xref>
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5 </xrefs>
10
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6 <macros>
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7 <import>macros.xml</import>
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8 </macros>
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9 <expand macro="requirements">
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10 <requirement type="package" version="1.20.2">r-getopt</requirement>
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11 <requirement type="package" version="0.2.20">r-rjson</requirement>
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12 </expand>
0
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13 <code file="dexseq_helper.py" />
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14 <stdio>
7
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15 <regex match="Execution halted"
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16 source="both"
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17 level="fatal"
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18 description="Execution halted." />
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19 <regex match="Input-Error 01"
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20 source="both"
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21 level="fatal"
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22 description="Error in your input parameters: Make sure you only apply factors to selected samples." />
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23 <regex match="Error in"
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24 source="both"
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25 level="fatal"
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26 description="An undefined error occured, please check your input carefully and contact your administrator." />
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27 <regex match="Error:"
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28 source="both"
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29 level="fatal"
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30 description="Error in the R script." />
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31 </stdio>
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32 <version_command><![CDATA[
0ccbed58b270 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dexseq commit 0a56599c36b4968095ec5a3cb589f94fb139466c
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33 echo $(R --version | grep version | grep -v GNU)", DEXSeq version" $(R --vanilla --slave -e "library(DEXSeq); cat(sessionInfo()\$otherPkgs\$DEXSeq\$Version)" 2> /dev/null | grep -v -i "WARNING: ")" (depends on DESeq2 "$(R --vanilla --slave -e "library(DESeq2); cat(sessionInfo()\$otherPkgs\$DESeq2\$Version)" 2> /dev/null | grep -v -i "WARNING: ")")"
0ccbed58b270 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dexseq commit 0a56599c36b4968095ec5a3cb589f94fb139466c
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34 ]]></version_command>
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35 <command><![CDATA[
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36 mkdir ./html_out &&
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37 #import json
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38 Rscript '$__tool_directory__/dexseq.R'
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39 -o '$dexseq_out'
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40 -p \${GALAXY_SLOTS:-4}
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41 #set $temp_factor_names = list()
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42 #for $factor in $rep_factorName:
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43 #set $temp_factor = list()
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44 #set $count_files1 = list()
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45 #for $file in $factor.countFiles1:
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46 $count_files1.append(str($file))
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47 #end for
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48 $temp_factor.append( {str($factor.factorLevel1): $count_files1} )
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49
8
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50 #set $count_files2 = list()
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51 #for $file in $factor.countFiles2:
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52 $count_files2.append(str($file))
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53 #end for
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54 $temp_factor.append( {str($factor.factorLevel2): $count_files2} )
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55 $temp_factor_names.append([str($factor.factorName), $temp_factor])
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56 #end for
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57 -f '#echo json.dumps(temp_factor_names)#'
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58 -a $gtf
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59 -c $fdr_cutoff
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60 -d $rds
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61
8
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62 #if $report:
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63 -r ./html_out
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64 &&
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65 mkdir '$htmlreport.extra_files_path'
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66 &&
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67 cp ./html_out/testForDEU.html $htmlreport
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68 &&
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69 cp -r ./html_out/* '$htmlreport.extra_files_path'
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70 #end if
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71 ]]></command>
0
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72 <inputs>
3
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73 <param name="gtf" type="data" format="gtf,gff" label="GTF file created from DEXSeq-Count tool"/>
0
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74 <repeat name="rep_factorName" title="Factor" min="1">
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75 <param name="factorName" type="text" value="FactorName" label="Specify a factor name"
0
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76 help="Only letters, numbers and underscores will be retained in this field">
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77 <sanitizer>
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78 <valid initial="string.letters,string.digits"><add value="_" /></valid>
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79 </sanitizer>
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80 </param>
5
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81 <param name="factorLevel1" type="text" value="FactorLevel1" label="Specify a factor level"
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82 help="Only letters, numbers and underscores will be retained in this field">
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83 <sanitizer>
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84 <valid initial="string.letters,string.digits"><add value="_" /></valid>
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85 </sanitizer>
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86 </param>
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87 <param name="countFiles1" type="data" format="tabular" multiple="true" label="Count file for factor level 1"/>
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88
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89 <param name="factorLevel2" type="text" value="FactorLevel2" label="Specify a factor level"
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90 help="Only letters, numbers and underscores will be retained in this field">
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91 <sanitizer>
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92 <valid initial="string.letters,string.digits"><add value="_" /></valid>
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93 </sanitizer>
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94 </param>
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95 <param name="countFiles2" type="data" format="tabular" multiple="true" label="Count files for factor level 2"/>
0
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96 </repeat>
4
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97 <param name="report" type="boolean" truevalue="True" falsevalue="False" checked="true"
0
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98 label="Visualise the analysis results?"
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99 help="Output an additional HTML file." />
11
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100 <param name="rds" type="boolean" truevalue="True" falsevalue="False" checked="false"
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101 label="Output rds file for plotDEXSeq?" help="Can be used to generate plots for individual genes with plotDEXSeq" />
4
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102 <param name="fdr_cutoff" type="float" min="0.0" max="1.0" value="0.05" label="All the genes under this FDR threshold will be shown in the html report"/>
0
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103 </inputs>
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104 <outputs>
10
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105 <data name="dexseq_out" format="tabular" label="DEXSeq result file on ${on_string}" />
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106 <data name="htmlreport" format="html" label="DEXSeq report on ${on_string}">
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107 <filter>report is True</filter>
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108 </data>
11
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109 <data name="rds_out" format="rdata" from_work_dir="DEXSeqResults.rds" label="DEXSeq rds file on ${on_string}">
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110 <filter>rds is True</filter>
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111 </data>
0
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112 </outputs>
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113 <tests>
8
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114 <!-- Ensure default output works-->
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115 <test expect_num_outputs="1">
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116 <param name="gtf" value="dexseq.gtf" ftype="gtf"/>
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117 <repeat name="rep_factorName">
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118 <param name="factorName" value="condition"/>
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119 <param name="factorLevel1" value="knockdown"/>
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120 <param name="countFiles1" value="treated1fb.txt,treated2fb.txt,treated3fb.txt" ftype="tabular"/>
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121 <param name="factorLevel2" value="control"/>
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122 <param name="countFiles2" value="untreated1fb.txt,untreated2fb.txt,untreated3fb.txt,untreated4fb.txt" ftype="tabular"/>
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123 </repeat>
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124 <param name="report" value="False"/>
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125 <param name="fdr_cutoff" value="1"/>
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126 <output name="dexseq_out" file="dexseq_result.tabular" ftype="tabular" compare="sim_size"/>
8
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127 </test>
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128 <!-- Ensure report works-->
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129 <test expect_num_outputs="2">
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130 <param name="gtf" value="dexseq.gtf" ftype="gtf"/>
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131 <repeat name="rep_factorName">
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132 <param name="factorName" value="Treatment"/>
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133 <param name="factorLevel1" value="knockdown"/>
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134 <param name="countFiles1" value="treated1fb.txt,treated2fb.txt,treated3fb.txt" ftype="tabular"/>
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135 <param name="factorLevel2" value="control"/>
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136 <param name="countFiles2" value="untreated1fb.txt,untreated2fb.txt,untreated3fb.txt,untreated4fb.txt" ftype="tabular"/>
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137 </repeat>
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138 <param name="report" value="True"/>
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139 <param name="fdr_cutoff" value="1"/>
10
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140 <output name="dexseq_out" file="dexseq_result.tabular" ftype="tabular" compare="sim_size"/>
8
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141 </test>
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142 <!-- Ensure two factors works-->
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143 <test expect_num_outputs="1">
5
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144 <param name="gtf" value="dexseq.gtf" ftype="gtf"/>
0
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145 <repeat name="rep_factorName">
4
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146 <param name="factorName" value="condition"/>
5
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147 <param name="factorLevel1" value="knockdown"/>
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148 <param name="countFiles1" value="treated1fb.txt,treated2fb.txt,treated3fb.txt" ftype="tabular"/>
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149 <param name="factorLevel2" value="control"/>
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150 <param name="countFiles2" value="untreated1fb.txt,untreated2fb.txt,untreated3fb.txt,untreated4fb.txt" ftype="tabular"/>
0
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151 </repeat>
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152 <repeat name="rep_factorName">
4
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153 <param name="factorName" value="libtype"/>
5
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154 <param name="factorLevel1" value="singleend"/>
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155 <param name="countFiles1" value="treated1fb.txt,untreated1fb.txt,untreated2fb.txt" ftype="tabular"/>
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156 <param name="factorLevel2" value="pairedend"/>
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157 <param name="countFiles2" value="treated2fb.txt,treated3fb.txt,untreated3fb.txt,untreated4fb.txt" ftype="tabular"/>
0
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158 </repeat>
4
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159 <param name="report" value="False"/>
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160 <param name="fdr_cutoff" value="0.05"/>
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161 <output name="dexseq_out" file="dexseq_result_2fact.tabular" ftype="tabular" compare="sim_size"/>
0
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162 </test>
11
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163 <!-- Ensure rds output works-->
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164 <test expect_num_outputs="2">
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165 <param name="gtf" value="dexseq.gtf" ftype="gtf"/>
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166 <repeat name="rep_factorName">
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167 <param name="factorName" value="condition"/>
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168 <param name="factorLevel1" value="knockdown"/>
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169 <param name="countFiles1" value="treated1fb.txt,treated2fb.txt,treated3fb.txt" ftype="tabular"/>
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170 <param name="factorLevel2" value="control"/>
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171 <param name="countFiles2" value="untreated1fb.txt,untreated2fb.txt,untreated3fb.txt,untreated4fb.txt" ftype="tabular"/>
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172 </repeat>
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173 <param name="report" value="False"/>
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174 <param name="rds" value="True"/>
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175 <param name="fdr_cutoff" value="0.05"/>
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176 <output name="dexseq_out" ftype="tabular" file="dexseq_result.tabular" compare="sim_size"/>
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177 <output name="rds_out" ftype="rdata" file="dexseq.rds" compare="sim_size"/>
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178 </test>
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179 </tests>
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180 <help><![CDATA[
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181 .. class:: infomark
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182
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183 **What it does**
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184
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185 Inference of differential exon usage in RNA-Seq.
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186
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187
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188 **Inputs**
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189
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190 DEXSeq_ takes count tables generated from the dexseq_count as input. Count tables must be generated for each sample individually.
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191 DEXSeq_ is capable of handling multiple factors that affect your experiment. The first factor you input is considered to be the primary
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192 factor that affects gene expressions. You can also input several secondary factors that might
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193 influence your experiment but the final output will be changes in genes due to primary factor in the presence of secondary factors. Each factor has two levels/states.
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194 You need to select an appropriate count table from your history for each factor level.
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195
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196 The following table gives some examples of factors and their levels:
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197
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198 ========= ============== ===============
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199 Factor Factor level 1 Factor level 2
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200 --------- -------------- ---------------
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201 condition Knockdown Wildtype
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202 --------- -------------- ---------------
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203 treatment Treated Untreated
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204 --------- -------------- ---------------
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205 timePoint Day4 Day1
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206 --------- -------------- ---------------
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207 SeqType SingleEnd PairedEnd
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208 --------- -------------- ---------------
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209 Gender Female Male
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210 ========= ============== ===============
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211
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212 *Note*: Output log2 fold changes are based on primary factor level 1 vs. factor level 2. Here the order of factor levels is important. For example, for the factor 'condition' given in the above table, DEXSeq computes fold changes of 'Knockdown' samples against 'Wildtype', i.e. the values correspond to up or down regulations of genes in Knockdown samples.
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213
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214 **Output**
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215
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216 DEXSeq_ generates a tabular file containing the different columns and an optional html report. It can also ouput the DEXSeqResults R object that can be used with the plotDEXSeq tool to visualise individual genes.
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217
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218 ====== ==========================================================
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219 Column Description
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220 ------ ----------------------------------------------------------
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221 1 Gene and exon Identifiers
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222 2 group/gene identifier
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223 3 feature/exon identifier
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224 4 mean of the counts across samples in each feature/exon
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225 5 exon dispersion estimate
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226 6 LRT statistic
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227 7 LRT p-value
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228 8 BH adjusted p-values
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229 9 exon usage coefficient factorLevel 2
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230 10 exon usage coefficient factorLevel 1
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231 11 relative exon usage fold changes
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232 12 GRanges object of the coordinates of the exon/feature
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233 13 matrix of integer counts, of each column containing a sample
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234 14 list of transcripts overlapping with the exon
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235 ====== ==========================================================
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236
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237
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238 .. _DEXSeq: http://master.bioconductor.org/packages/release/bioc/html/DEXSeq.html
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239 ]]></help>
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240 <citations>
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241 <citation type="doi">10.1101/gr.133744.111</citation>
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242 </citations>
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243 </tool>