Mercurial > repos > iuc > deseq2

annotate deseq2.xml @ 27:dc6bc19f05ab draft

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 83eb5b2665d87c02b270596f8175499e69061032
author iuc
date Sat, 19 May 2018 03:55:30 -0400
parents 47267a044ef1
children 5707d3c668d6
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1 <tool id="deseq2" name="DESeq2" version="2.11.40.2">
0
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2 <description>Determines differentially expressed features from count tables</description>
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3 <requirements>
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4 <requirement type="package" version="1.18.1">bioconductor-deseq2</requirement>
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5 <requirement type="package" version="1.6.0">bioconductor-tximport</requirement>
25
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6 <requirement type="package" version="1.30.0">bioconductor-genomicfeatures</requirement>
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7 <requirement type="package" version="0.6.5">r-ggrepel</requirement>
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8 <requirement type="package" version="1.0.8">r-pheatmap</requirement>
0
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9 </requirements>
6
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10 <stdio>
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11 <regex match="Execution halted"
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12 source="both"
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13 level="fatal"
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14 description="Execution halted." />
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15 <regex match="Error in"
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16 source="both"
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17 level="fatal"
19
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18 description="An undefined error occurred, please check your input carefully and contact your administrator." />
6
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19 <regex match="Fatal error"
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20 source="both"
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21 level="fatal"
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22 description="An undefined error occurred, please check your input carefully and contact your administrator." />
6
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23 </stdio>
23
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24 <version_command><![CDATA[
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25 echo $(R --version | grep version | grep -v GNU)", DESeq2 version" $(R --vanilla --slave -e "library(DESeq2); cat(sessionInfo()\$otherPkgs\$DESeq2\$Version)" 2> /dev/null | grep -v -i "WARNING: ")
d1241c2b81a7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 0a56599c36b4968095ec5a3cb589f94fb139466c
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26 ]]></version_command>
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27 <command><![CDATA[
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28 #if $tximport.tximport_selector == 'tximport':
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29 #if $tximport.mapping_format.mapping_format_selector == 'gtf':
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30 ln -s '$tximport.mapping_format.gtf_file' mapping.gtf &&
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31 #else:
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32 ln -s '$tximport.mapping_format.tabular_file' mapping.txt &&
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33 #end if
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34 #end if
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35
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36 #import json
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37 #import os
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38 Rscript '${__tool_directory__}/deseq2.R'
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39 -o '$deseq_out'
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40 #if $pdf:
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41 -p '$plots'
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42 #end if
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43 #if $normCounts:
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44 -n '$counts_out'
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45 #end if
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46 #set $filename_to_element_identifiers = {}
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47 #set $temp_factor_names = list()
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48 #for $factor in $rep_factorName:
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49 #set $temp_factor = list()
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50 #for $level in $factor.rep_factorLevel:
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51 #set $count_files = list()
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52 #for $file in $level.countsFile:
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53 $count_files.append(str($file))
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54 $filename_to_element_identifiers.__setitem__(os.path.basename(str($file)), $file.element_identifier)
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55 #end for
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56 $temp_factor.append( {str($level.factorLevel): $count_files} )
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57 #end for
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58 $temp_factor.reverse()
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59 $temp_factor_names.append([str($factor.factorName), $temp_factor])
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60 #end for
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61
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62 $header
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63
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64 -f '#echo json.dumps(temp_factor_names)#'
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65 -l '#echo json.dumps(filename_to_element_identifiers)#'
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66 -t $fit_type
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67 #if $outlier_replace_off:
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68 -a
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69 #end if
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70 #if $outlier_filter_off:
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71 -b
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72 #end if
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73 #if $auto_mean_filter_off:
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74 -c
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75 #end if
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76 #if $many_contrasts:
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77 -m
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78 #end if
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79 #if $tximport.tximport_selector == 'tximport':
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80 -i
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81 -y $tximport.txtype
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82 #if $tximport.mapping_format.mapping_format_selector == 'gtf':
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83 -x mapping.gtf
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84 #else:
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85 -x mapping.txt
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86 #end if
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87
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88 #end if
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89 ]]></command>
0
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90 <inputs>
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91 <repeat name="rep_factorName" title="Factor" min="1">
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92 <param name="factorName" type="text" value="FactorName" label="Specify a factor name, e.g. effects_drug_x or cancer_markers"
0
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93 help="Only letters, numbers and underscores will be retained in this field">
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94 <sanitizer>
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95 <valid initial="string.letters,string.digits"><add value="_" /></valid>
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96 </sanitizer>
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97 </param>
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98 <repeat name="rep_factorLevel" title="Factor level" min="2" default="2">
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99 <param name="factorLevel" type="text" value="FactorLevel" label="Specify a factor level, typical values could be 'tumor', 'normal', 'treated' or 'control'"
0
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100 help="Only letters, numbers and underscores will be retained in this field">
5b1ce9db498e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 76d0758966bde7c15f5224913cee5e8ce28314ff
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101 <sanitizer>
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102 <valid initial="string.letters,string.digits"><add value="_" /></valid>
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103 </sanitizer>
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104 </param>
6
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105 <param name="countsFile" type="data" format="tabular" multiple="true" label="Counts file(s)"/>
0
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106 </repeat>
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107 </repeat>
17
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108
27
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109 <param name="header" type="boolean" truevalue="-H" falsevalue="" checked="true" label="Files have header?" help="If this option is set to Yes, the tool will assume that the count files have column headers in the first row. Default: Yes" />
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110
17
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111 <conditional name="tximport">
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112 <param name="tximport_selector" type="select" label="Choice of Input data">
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113 <option value="count" selected="True">Count data (e.g. from HTSeq-count, featureCounts or StringTie)</option>
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114 <option value="tximport">TPM values (e.g. from kallisto, sailfish or salmon)</option>
17
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115 </param>
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116 <when value="tximport">
23
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117 <param name="txtype" type="select" label="Program used to generate TPMs">
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118 <option value="kallisto">kallisto</option>
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119 <option value="sailfish">Sailfish</option>
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120 <option value="salmon">Salmon</option>
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121 </param>
17
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122 <conditional name="mapping_format">
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123 <param name="mapping_format_selector" type="select" label="Gene mapping format">
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124 <option value="gtf" selected="True">GTF</option>
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125 <option value="tabular">Transcript-ID and Gene-ID mapping file</option>
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126 </param>
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127 <when value="gtf">
23
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128 <param name="gtf_file" type="data" format="gtf,gff3" label="GTF/GFF3 file with Transcript - Gene mapping"/>
17
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129 </when>
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130 <when value="tabular">
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131 <param name="tabular_file" type="data" format="tabular" label="Tabular file with Transcript - Gene mapping"/>
17
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132 </when>
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133 </conditional>
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134 </when>
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135 <when value="count" />
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136 </conditional>
0
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137 <param name="pdf" type="boolean" truevalue="1" falsevalue="0" checked="true"
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138 label="Visualising the analysis results"
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139 help="output an additional PDF files" />
17
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140 <param name="normCounts" type="boolean" truevalue="1" falsevalue="0" checked="false"
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141 label="Output normalized counts table" />
0
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142 <param name="many_contrasts" type="boolean" truevalue="1" falsevalue="0" checked="false"
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143 label="Output all levels vs all levels of primary factor (use when you have >2 levels for primary factor)"
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144 help=" DESeq2 performs independent filtering by default using the mean of normalized counts as a filter statistic" />
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145 <param name="fit_type" type="select" label="Fit type">
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146 <option value="1" selected="true">parametric</option>
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147 <option value="2">local</option>
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148 <option value="3">mean</option>
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149 </param>
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150 <param name="outlier_replace_off" type="boolean" truevalue="1" falsevalue="0" checked="false"
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151 label="Turn off outliers replacement (only affects with >6 replicates)"
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152 help="When there are more than 6 replicates for a given sample, the DESeq2 will automatically replace
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153 counts with large Cook’s distance with the trimmed mean over all samples, scaled up by the size factor
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154 or normalization factor for that sample" />
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155 <param name="outlier_filter_off" type="boolean" truevalue="1" falsevalue="0" checked="false"
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156 label="Turn off outliers filtering (only affects with >2 replicates)"
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157 help="When there are more than 2 replicates for a given sample, the DESeq2 will automatically
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158 filter genes which contain a Cook’s distance above a cutoff" />
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159 <param name="auto_mean_filter_off" type="boolean" truevalue="1" falsevalue="0" checked="false"
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160 label="Turn off independent filtering"
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161 help=" DESeq2 performs independent filtering by default using the mean of normalized counts as a filter statistic" />
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162 </inputs>
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163 <outputs>
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164 <data format="tabular" name="deseq_out" label="DESeq2 result file on ${on_string}">
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165 <filter>many_contrasts is False</filter>
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166 <actions>
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167 <action name="column_names" type="metadata" default="GeneID,Base mean,log2(FC),StdErr,Wald-Stats,P-value,P-adj" />
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168 </actions>
0
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169 </data>
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170 <collection name="split_output" type="list" label="DESeq2 result files on ${on_string}">
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171 <filter>many_contrasts is True</filter>
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172 <discover_datasets pattern="None.(?P&lt;designation&gt;.+_vs_.+)" format="tabular" directory="." visible="false"/>
0
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173 </collection>
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174 <data format="pdf" name="plots" label="DESeq2 plots on ${on_string}">
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175 <filter>pdf == True</filter>
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176 </data>
17
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177 <data format="tabular" name="counts_out" label="Normalized counts file on ${on_string}">
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178 <filter>normCounts == True</filter>
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179 </data>
0
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180 </outputs>
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181 <tests>
27
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182 <!--Ensure counts files with header works -->
23
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183 <test expect_num_outputs="2">
0
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184 <repeat name="rep_factorName">
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185 <param name="factorName" value="Treatment"/>
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186 <repeat name="rep_factorLevel">
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187 <param name="factorLevel" value="Treated"/>
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188 <param name="countsFile" value="GSM461179_treat_single.counts,GSM461180_treat_paired.counts,GSM461181_treat_paired.counts"/>
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189 </repeat>
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190 <repeat name="rep_factorLevel">
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191 <param name="factorLevel" value="Untreated"/>
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192 <param name="countsFile" value="GSM461176_untreat_single.counts,GSM461177_untreat_paired.counts,GSM461178_untreat_paired.counts,GSM461182_untreat_single.counts"/>
5b1ce9db498e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 76d0758966bde7c15f5224913cee5e8ce28314ff
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193 </repeat>
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194 </repeat>
17
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195 <param name="pdf" value="False"/>
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196 <param name="normCounts" value="True"/>
27
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197 <output name="counts_out">
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198 <assert_contents>
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199 <has_text_matching expression="untreat1\tuntreat2\tuntreat3\tuntreat4\ttreat1\ttreat2\ttreat3" />
dc6bc19f05ab planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 83eb5b2665d87c02b270596f8175499e69061032
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200 <has_text_matching expression="FBgn0000003\t0\t0\t0\t0\t0\t0\t0" />
dc6bc19f05ab planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 83eb5b2665d87c02b270596f8175499e69061032
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201 </assert_contents>
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202 </output>
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203 <output name="deseq_out" >
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204 <assert_contents>
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205 <has_text_matching expression="FBgn0003360\t1933.9504.*\t-2.8399.*\t0.1309.*-21.6851.*2.831.*8.024" />
dc6bc19f05ab planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 83eb5b2665d87c02b270596f8175499e69061032
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206 </assert_contents>
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207 </output>
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208 </test>
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209 <!--Ensure counts files without header works -->
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210 <test expect_num_outputs="2">
dc6bc19f05ab planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 83eb5b2665d87c02b270596f8175499e69061032
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211 <repeat name="rep_factorName">
dc6bc19f05ab planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 83eb5b2665d87c02b270596f8175499e69061032
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212 <param name="factorName" value="Treatment"/>
dc6bc19f05ab planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 83eb5b2665d87c02b270596f8175499e69061032
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213 <repeat name="rep_factorLevel">
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214 <param name="factorLevel" value="Treated"/>
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215 <param name="countsFile" value="GSM461179_treat_single.counts.noheader,GSM461180_treat_paired.counts.noheader,GSM461181_treat_paired.counts.noheader"/>
dc6bc19f05ab planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 83eb5b2665d87c02b270596f8175499e69061032
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diff changeset
216 </repeat>
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217 <repeat name="rep_factorLevel">
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218 <param name="factorLevel" value="Untreated"/>
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219 <param name="countsFile" value="GSM461176_untreat_single.counts.noheader,GSM461177_untreat_paired.counts.noheader,GSM461178_untreat_paired.counts.noheader,GSM461182_untreat_single.counts.noheader"/>
dc6bc19f05ab planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 83eb5b2665d87c02b270596f8175499e69061032
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220 </repeat>
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221 </repeat>
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222 <param name="header" value="False"/>
dc6bc19f05ab planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 83eb5b2665d87c02b270596f8175499e69061032
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223 <param name="pdf" value="False"/>
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224 <param name="normCounts" value="True"/>
dc6bc19f05ab planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 83eb5b2665d87c02b270596f8175499e69061032
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225 <output name="counts_out">
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226 <assert_contents>
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227 <has_text_matching expression="GSM461176_untreat_single.counts.noheader\tGSM461177_untreat_paired.counts.noheader\tGSM461178_untreat_paired.counts.noheader\tGSM461182_untreat_single.counts.noheader\tGSM461179_treat_single.counts.noheader\tGSM461180_treat_paired.counts.noheader\tGSM461181_treat_paired.counts.noheader" />
dc6bc19f05ab planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 83eb5b2665d87c02b270596f8175499e69061032
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228 <has_text_matching expression="FBgn0000003\t0\t0\t0\t0\t0\t0\t0" />
dc6bc19f05ab planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 83eb5b2665d87c02b270596f8175499e69061032
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229 </assert_contents>
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230 </output>
dc6bc19f05ab planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 83eb5b2665d87c02b270596f8175499e69061032
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231 <output name="deseq_out" >
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232 <assert_contents>
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233 <has_text_matching expression="FBgn0003360\t1933.9504.*\t-2.8399.*\t0.1309.*-21.6851.*2.831.*8.024" />
dc6bc19f05ab planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 83eb5b2665d87c02b270596f8175499e69061032
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234 </assert_contents>
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235 </output>
17
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236 </test>
23
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237 <!--Ensure Sailfish/Salmon input with tx2gene table works-->
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238 <test expect_num_outputs="1">
17
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239 <repeat name="rep_factorName">
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240 <param name="factorName" value="Treatment"/>
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diff changeset
241 <repeat name="rep_factorLevel">
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242 <param name="factorLevel" value="Treated"/>
23
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243 <param name="countsFile" value="sailfish/sailfish_quant.sf1.tab,sailfish/sailfish_quant.sf2.tab,sailfish/sailfish_quant.sf3.tab"/>
17
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diff changeset
244 </repeat>
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245 <repeat name="rep_factorLevel">
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246 <param name="factorLevel" value="Untreated"/>
23
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247 <param name="countsFile" value="sailfish/sailfish_quant.sf4.tab,sailfish/sailfish_quant.sf5.tab,sailfish/sailfish_quant.sf6.tab"/>
17
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diff changeset
248 </repeat>
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diff changeset
249 </repeat>
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diff changeset
250 <param name="pdf" value="False"/>
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251 <param name="tximport_selector" value="tximport"/>
23
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252 <param name="txtype" value="sailfish"/>
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253 <param name="mapping_format_selector" value="tabular"/>
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254 <param name="tabular_file" value="tx2gene.tab"/>
27
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diff changeset
255 <output name="deseq_out" >
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256 <assert_contents>
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257 <has_text_matching expression="MIR6859-2\t1.1858.*\t-1.5832.*\t1.2956.*\t-1.2219.*\t0.2217.*\t0.8868.*" />
dc6bc19f05ab planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 83eb5b2665d87c02b270596f8175499e69061032
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258 </assert_contents>
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259 </output>
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260 </test>
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261 </tests>
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262 <help><![CDATA[
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263 .. class:: infomark
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264
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265 **What it does**
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266
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267 Estimate variance-mean dependence in count data from high-throughput sequencing assays and test for differential expression based on a model using the negative binomial distribution
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268
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269 -----
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270
0
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271 **Inputs**
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272
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273 **Count Files**
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274
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275 DESeq2_ takes count tables generated from **featureCounts**, **HTSeq-count** or **StringTie** as input. Count tables must be generated for each sample individually. One header row is assumed, but files with no header (e.g from HTSeq) can be input with the *Files have header?* option set to No. DESeq2 is capable of handling multiple factors that affect your experiment. The first factor you input is considered as the primary factor that affects gene expressions. Optionally, you can input one or more secondary factors that might influence your experiment. But the final output will be changes in genes due to primary factor in presence of secondary factors. Each factor has two levels/states. You need to select appropriate count table from your history for each factor level.
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276
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277 The following table gives some examples of factors and their levels:
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278
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279 ========= ============== ===============
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280 Factor Factor level 1 Factor level 2
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281 --------- -------------- ---------------
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282 Treatment Treated Untreated
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283 --------- -------------- ---------------
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284 Condition Knockdown Wildtype
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285 --------- -------------- ---------------
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286 TimePoint Day4 Day1
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287 --------- -------------- ---------------
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288 SeqType SingleEnd PairedEnd
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289 --------- -------------- ---------------
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290 Gender Female Male
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291 ========= ============== ===============
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292
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293 *Note*: Output log2 fold changes are based on primary factor level 1 vs. factor level2. Here the order of factor levels is important. For example, for the factor 'Treatment' given in above table, DESeq2 computes fold changes of 'Treated' samples against 'Untreated', i.e. the values correspond to up or down regulations of genes in Treated samples.
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294
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295 DESeq2_ can also take transcript-level counts from quantification tools such as, **kallisto**, **Salmon** and **Sailfish**, and this Galaxy wrapper incorporates the Bioconductor tximport_ package to process the transcript counts for DESeq2.
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296
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297 **Salmon or Sailfish Files**
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298
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299 Salmon or Sailfish ``quant.sf`` files can be imported by setting type to *Salmon* or *Sailfish* respectively above. Note: for previous version of Salmon or Sailfish, in which the quant.sf files start with comment lines you will need to remove the comment lines before inputting here. An example of the format is shown below.
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300
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301 Example:
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302
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303 ============ ========== =============== =========== ===========
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304 Name Length EffectiveLength TPM NumReads
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305 ------------ ---------- --------------- ----------- -----------
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306 NR_001526 164 20.4518 0 0
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307 NR_001526_1 164 20.4518 0 0
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308 NR_001526_2 164 20.4518 0 0
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309 NM_130786 1764 1956.04 2.47415 109.165
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310 NR_015380 2129 2139.53 1.77331 85.5821
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311 NM_001198818 9360 7796.58 2.38616e-07 4.19648e-05
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312 NM_001198819 9527 7964.62 0 0
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313 NM_001198820 9410 7855.78 0 0
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314 NM_014576 9267 7714.88 0.0481114 8.37255
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315 ============ ========== =============== =========== ===========
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316
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317 **kallisto Files**
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318
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319 kallisto ``abundance.tsv`` files can be imported by setting type to *kallisto* above. An example of the format is shown below.
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320
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321 Example:
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322
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323 ============ ========== =============== =========== ===========
27
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324 target_id length eff_length est_counts tpm
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325 ------------ ---------- --------------- ----------- -----------
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326 NR_001526 164 20.4518 0 0
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327 NR_001526_1 164 20.4518 0 0
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328 NR_001526_2 164 20.4518 0 0
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329 NM_130786 1764 1956.04 109.165 2.47415
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330 NR_015380 2129 2139.53 85.5821 1.77331
23
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331 NM_001198818 9360 7796.58 4.19648e-05 2.38616e-07
27
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332 NM_001198819 9527 7964.62 0 0
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333 NM_001198820 9410 7855.78 0 0
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334 NM_014576 9267 7714.88 8.37255 0.0481114
23
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335 ============ ========== =============== =========== ===========
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336
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337 -----
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338
0
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339 **Output**
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340
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341 DESeq2_ generates a tabular file containing the different columns and optional visualized results as PDF.
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342
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343 ====== ==========================================================
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344 Column Description
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345 ------ ----------------------------------------------------------
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346 1 Gene Identifiers
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347 2 mean normalised counts, averaged over all samples from both conditions
5b1ce9db498e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 76d0758966bde7c15f5224913cee5e8ce28314ff
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348 3 the logarithm (to basis 2) of the fold change (See the note in inputs section)
5b1ce9db498e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 76d0758966bde7c15f5224913cee5e8ce28314ff
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349 4 standard error estimate for the log2 fold change estimate
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350 5 Wald statistic
00ff49f9e750 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 1c0a208ea184713d5b38c5575861ecf6ce7decdd
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351 6 p value for the statistical significance of this change
00ff49f9e750 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 1c0a208ea184713d5b38c5575861ecf6ce7decdd
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352 7 p value adjusted for multiple testing with the Benjamini-Hochberg procedure
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5b1ce9db498e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 76d0758966bde7c15f5224913cee5e8ce28314ff
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353 which controls false discovery rate (FDR)
5b1ce9db498e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 76d0758966bde7c15f5224913cee5e8ce28314ff
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354 ====== ==========================================================
5b1ce9db498e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 76d0758966bde7c15f5224913cee5e8ce28314ff
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355
5b1ce9db498e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 76d0758966bde7c15f5224913cee5e8ce28314ff
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356 .. _DESeq2: http://master.bioconductor.org/packages/release/bioc/html/DESeq2.html
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357 .. _tximport: https://bioconductor.org/packages/devel/bioc/vignettes/tximport/inst/doc/tximport.html
d1241c2b81a7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 0a56599c36b4968095ec5a3cb589f94fb139466c
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358 ]]></help>
0
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359 <citations>
5b1ce9db498e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 76d0758966bde7c15f5224913cee5e8ce28314ff
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360 <citation type="doi">10.1186/s13059-014-0550-8</citation>
5b1ce9db498e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 76d0758966bde7c15f5224913cee5e8ce28314ff
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361 </citations>
5b1ce9db498e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 76d0758966bde7c15f5224913cee5e8ce28314ff
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362 </tool>