annotate deepvariant.xml @ 4:09fff9439112 draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
author iuc
date Mon, 02 Feb 2026 12:34:03 +0000
parents 1a3c1f1a4d24
children
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09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
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1 <tool id='deepvariant' name='DeepVariant' version='@TOOL_VERSION@+galaxy@SUFFIX_VERSION@' profile='@PROFILE@'>
0
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2 <description>deep learning-based variant caller</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro='edam_ontology' />
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7 <expand macro='requirements' />
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8 <command detect_errors='exit_code'><![CDATA[
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9 ln -s '${reads}' reads_alignment.bam
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10 && ln -s '${reads.metadata.bam_index}' reads_alignment.bam.bai
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11 #if $regions_conditional.regions_option == 'bed'
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12 && ln -s '${regions_conditional.bed_file}' region.bed
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13 #end if
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
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14 #if $par_regions_bed
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15 && ln -s '${par_regions_bed}' par_regions.bed
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16 #end if
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17 #set $logging_dir_value = None
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18 #if $create_runtime_report
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19 #set $logging_dir_value = 'logging'
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20 && mkdir -p '$logging_dir_value'
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21 #end if
0
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22 #if $reference_genome.source == 'history':
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23 #set $ref_genome = 'reference.fasta'
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24 && ln -s -f '${reference_genome.history_item}' $ref_genome
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25 && samtools faidx $ref_genome
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26 #else:
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27 #set $ref_genome = $reference_genome.index.fields.path
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28 #end if
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29 && run_deepvariant
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30 --model_type=$model_type
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31 --ref=$ref_genome
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32 --reads='reads_alignment.bam'
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33 #if $sample_name
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34 --sample_name '$sample_name'
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35 #end if
0
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36 --output_vcf='./output.vcf.gz'
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37 #if $output_gvcf
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38 --output_gvcf='./output.g.vcf.gz'
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39 #end if
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40 #if $regions_conditional.regions_option == 'region'
4
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41 --regions '$regions_conditional.region_literal'
0
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42 #else if $regions_conditional.regions_option == 'bed'
4
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43 --regions 'region.bed'
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44 #end if
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45 --disable_small_model=$disable_small_model
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46 #if $haploid_contigs
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47 --haploid_contigs='$haploid_contigs'
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48 #end if
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49 #if $par_regions_bed
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50 --par_regions_bed='par_regions.bed'
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51 #end if
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52 #if $logging_dir_value
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53 --logging_dir='$logging_dir_value'
0
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54 #end if
4
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55 #if $report_title
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56 --report_title='$report_title'
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57 #end if
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58 --runtime_report=$create_runtime_report
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59 #if $make_examples_extra_args
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60 --make_examples_extra_args='$make_examples_extra_args'
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61 #end if
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62 #if $call_variants_extra_args
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63 --call_variants_extra_args='$call_variants_extra_args'
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64 #end if
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65 #if $postprocess_variants_extra_args
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66 --postprocess_variants_extra_args='$postprocess_variants_extra_args'
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67 #end if
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68 $vcf_stats_report
0
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69 --num_shards=\${GALAXY_SLOTS:-2}
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70 && gunzip './output.vcf.gz'
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71 #if $output_gvcf
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72 && gunzip './output.g.vcf.gz'
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73 #end if
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74 ]]> </command>
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75 <inputs>
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76 <conditional name="reference_genome">
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77 <param name="source" type="select" label="Source for the reference genome" help="Built-in references were created using default options.">
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78 <option value="indexed" selected="true">Use a built-in genome</option>
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79 <option value="history">Use a genome from history</option>
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80 </param>
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81 <when value="indexed">
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82 <param name="index" type="select" label="Select a reference genome" help="If your genome of interest is not listed, contact the Galaxy team.">
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83 <options from_data_table="fasta_indexes">
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84 <filter type="sort_by" column="2" />
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85 <validator type="no_options" message="No genomes are available for the selected input dataset" />
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86 </options>
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87 </param>
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88 </when>
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89 <when value="history">
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90 <param name="history_item" type="data" format="fasta" label="Reference genome" help="A reference genome in FASTA format" />
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91 </when>
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92 </conditional>
4
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93 <param argument="--reads" type="data" format="bam" label="BAM file" help="An aligned reads file in BAM format. The reads must be aligned to the reference genome" />
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94 <param argument="--sample_name" type="text" optional="true" label="Sample name" help="Sample name to use instead of the SM tag in the BAM header. Example: NA12878" />
0
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95 <param argument="--model_type" type="select" label="Model type" help="Type of model to use for variant calling">
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96 <option value="WGS">WGS: Illumina whole genome sequencing</option>
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97 <option value="WES">WES: Illumina whole exome sequencing</option>
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98 <option value="PACBIO">PacBio HiFi</option>
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99 <option value="HYBRID_PACBIO_ILLUMINA">Hybrid PacBio HiFi-Illumina</option>
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100 <option value="ONT_R104">ONT R10.4 simplex and duplex data</option>
0
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101 </param>
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102 <conditional name="regions_conditional">
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103 <param name="regions_option" type="select" label="Select specific regions to process" help="Restrict the analysis to specific regions. A space-separated list of chromosome regions to process. Individual elements can be region literals, such as chr20:10-20 or paths to BED files.">
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104 <option value="disabled" selected="True">Disabled</option>
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105 <option value="region">Specify region literals</option>
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106 <option value="bed">Provide a BED file</option>
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107 </param>
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108 <when value="disabled"/>
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109 <when value="region">
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110 <param name="region_literal" argument="--regions" type="text" label="Regions" help="This option refers to contigs present in the reference genome. These arguments accept space-separated lists, so all of the follow examples are valid arguments: 'chr20:10,000,000-11,000,000', 'chr20 chr21' and 'chr20'">
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111 <sanitizer invalid_char="">
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112 <valid initial="string.letters,string.digits">
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113 <add value="," />
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114 <add value=":" />
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115 <add value="-" />
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116 </valid>
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117 </sanitizer>
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118 <validator type="regex">[0-9a-zA-Z,:-]+</validator>
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119 </param>
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120 </when>
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121 <when value="bed">
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122 <param name="bed_file" argument="--regions" type="data" format="bed" label="BED file" help="The BED should the store genomic regions of interest" />
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123 </when>
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124 </conditional>
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125 <param argument="--output_gvcf" type="boolean" truevalue="True" falsevalue="False" checked="False" label="Generate genomic VCF (gVCF) output" help="The key difference between a regular VCF and a gVCF is that the gVCF has records for all sites, whether there is a variant call there or not. The goal is to have every site represented in the file in order to do joint analysis of a cohort in subsequent steps" />
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126 <section name="advanced_options" title="Advanced options" expanded="false">
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127 <param argument="--disable_small_model" type="boolean" truevalue="True" falsevalue="False" checked="False" label="Disable small model" help="Disable the use of the small model to call variants during make_examples." />
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128 <param argument="--haploid_contigs" type="text" optional="true" label="Haploid contigs" help="Comma-separated list of non-autosomal chromosomes. Example: chrX,chrY" />
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129 <param argument="--par_regions_bed" type="data" format="bed" optional="true" label="PAR regions BED file" help="BED file of pseudoautosomal regions." />
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130 <param argument="--report_title" type="text" optional="true" label="Report title" help="Title for the VCF stats report (HTML). Example: NA12878 DeepVariant report" />
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131 <param argument="--vcf_stats_report" type="boolean" truevalue="--vcf_stats_report=True" falsevalue="--vcf_stats_report=False" checked="False" label="Create VCF stats report (HTML)" help="Output a visual report (HTML) of statistics about the output VCF." />
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132 <param name="create_runtime_report" argument="--runtime_report" type="boolean" truevalue="True" falsevalue="False" checked="False" label="Create runtime report" help="Output make_examples runtime metrics and create a visual runtime report." />
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133 </section>
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134 <section name="expert_options" title="Expert options (may increase runtime and disk usage)" expanded="false">
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135 <param argument="--make_examples_extra_args" type="text" optional="true" label="make_examples extra args" help="Comma-separated flag_name=flag_value for make_examples.py. Example: min_base_quality=10,min_mapping_quality=5" />
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136 <param argument="--call_variants_extra_args" type="text" optional="true" label="call_variants extra args" help="Comma-separated flag_name=flag_value for call_variants.py. Example: allow_empty_examples=true,batch_size=1024" />
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137 <param argument="--postprocess_variants_extra_args" type="text" optional="true" label="postprocess_variants extra args" help="Comma-separated flag_name=flag_value for postprocess_variants.py. Example: cnn_homref_call_min_gq=20.0" />
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138 </section>
0
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139 </inputs>
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140 <outputs>
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141 <data name="vcf_file" format="vcf" from_work_dir="output.vcf" label="${tool.name} on ${on_string}: VCF file"/>
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142 <data name="html_report" format="html" from_work_dir="output.visual_report.html" label="${tool.name} on ${on_string}: HTML report">
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143 <filter>advanced_options['vcf_stats_report']</filter>
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144 </data>
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145 <data name="runtime_report" format="html" from_work_dir="logging/make_examples_runtime_by_region_report.html" label="${tool.name} on ${on_string}: runtime report">
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146 <filter>advanced_options['create_runtime_report']</filter>
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147 </data>
0
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148 <data name="gvcf_file" format="vcf" from_work_dir="output.g.vcf" label="${tool.name} on ${on_string}: gVCF file">
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149 <filter>output_gvcf</filter>
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150 </data>
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151 </outputs>
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152 <tests>
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153 <test expect_num_outputs="2">
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154 <conditional name="reference_genome">
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155 <param name="source" value="history"/>
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156 <param name="history_item" value="reference.fasta"/>
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157 </conditional>
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158 <param name="reads" value="reads.bam"/>
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159 <param name="model_type" value="WGS"/>
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160 <param name="output_gvcf" value="False"/>
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161 <conditional name="regions_conditional">
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162 <param name="regions_option" value="disabled"/>
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163 </conditional>
4
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164 <section name="advanced_options">
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165 <param name="vcf_stats_report" value="True"/>
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166 <param name="report_title" value="Test Report Title"/>
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167 </section>
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168 <output name="vcf_file" ftype="vcf">
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
169 <assert_contents>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
170 <has_text text="##fileformat=VCFv4.2"/>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
171 <has_text text="#CHROM"/>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
172 </assert_contents>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
173 </output>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
174 <output name="html_report" ftype="html">
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
175 <assert_contents>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
176 <is_valid_xml />
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
177 <has_n_lines n="34" delta="10" />
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
178 <has_text text="Test Report Title"/>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
179 </assert_contents>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
180 </output>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
181 </test>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
182 <!-- Test region literal option-->
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
183 <test expect_num_outputs="2">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
184 <conditional name="reference_genome">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
185 <param name="source" value="history"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
186 <param name="history_item" value="reference.fasta"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
187 </conditional>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
188 <param name="reads" value="reads.bam"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
189 <param name="model_type" value="WGS"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
190 <conditional name="regions_conditional">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
191 <param name="regions_option" value="region"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
192 <param name="region_literal" value="K03455:1-2669"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
193 </conditional>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
194 <section name="advanced_options">
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
195 <param name="vcf_stats_report" value="True"/>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
196 </section>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
197 <output name="vcf_file" ftype="vcf">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
198 <assert_contents>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
199 <has_text text="#CHROM"/>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
200 <has_text text="##fileformat=VCFv4.2"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
201 </assert_contents>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
202 </output>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
203 <output name="html_report" ftype="html">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
204 <assert_contents>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
205 <is_valid_xml />
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
206 <has_n_lines n="34" delta="10" />
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
207 <has_text text="Variant types"/>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
208 </assert_contents>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
209 </output>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
210 </test>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
211 <!-- Test region bed option-->
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
212 <test expect_num_outputs="2">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
213 <conditional name="reference_genome">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
214 <param name="source" value="history"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
215 <param name="history_item" value="reference.fasta"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
216 </conditional>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
217 <param name="reads" value="reads.bam"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
218 <param name="model_type" value="WGS"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
219 <conditional name="regions_conditional">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
220 <param name="regions_option" value="bed"/>
2
1071eaa424a2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit 4255237ed7a51299e25bd7de7ad52ac5d419a56c
iuc
parents: 1
diff changeset
221 <param name="bed_file" value="region.bed" ftype="bed"/>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
222 </conditional>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
223 <section name="advanced_options">
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
224 <param name="vcf_stats_report" value="True"/>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
225 </section>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
226 <output name="vcf_file" ftype="vcf">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
227 <assert_contents>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
228 <has_text text="#CHROM"/>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
229 <has_text text="##fileformat=VCFv4.2"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
230 </assert_contents>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
231 </output>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
232 <output name="html_report" ftype="html">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
233 <assert_contents>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
234 <is_valid_xml />
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
235 <has_n_lines n="34" delta="10" />
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
236 <has_text text="Variant types"/>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
237 </assert_contents>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
238 </output>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
239 </test>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
240 <!-- Test gvcf output option-->
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
241 <test expect_num_outputs="3">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
242 <conditional name="reference_genome">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
243 <param name="source" value="history"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
244 <param name="history_item" value="reference.fasta"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
245 </conditional>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
246 <param name="reads" value="reads.bam"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
247 <param name="model_type" value="WGS"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
248 <param name="output_gvcf" value="True"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
249 <conditional name="regions_conditional">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
250 <param name="regions_option" value="region"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
251 <param name="region_literal" value="K03455:1-2669"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
252 </conditional>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
253 <section name="advanced_options">
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
254 <param name="vcf_stats_report" value="True"/>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
255 </section>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
256 <output name="vcf_file" ftype="vcf">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
257 <assert_contents>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
258 <has_text text="#CHROM"/>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
259 <has_text text="##fileformat=VCFv4.2"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
260 </assert_contents>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
261 </output>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
262 <output name="gvcf_file" ftype="vcf">
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
263 <assert_contents>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
264 <has_text text="#CHROM"/>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
265 <has_text text="##fileformat=VCFv4.2"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
266 </assert_contents>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
267 </output>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
268 <output name="html_report" ftype="html">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
269 <assert_contents>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
270 <is_valid_xml />
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
271 <has_n_lines n="34" delta="10" />
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
272 <has_text text="Variant types"/>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
273 </assert_contents>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
274 </output>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
275 </test>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
276 <!-- Test CRAM format input-->
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
277 <test expect_num_outputs="2">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
278 <conditional name="reference_genome">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
279 <param name="source" value="history"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
280 <param name="history_item" value="reference.fasta"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
281 </conditional>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
282 <param name="reads" value="reads.cram"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
283 <param name="model_type" value="WGS"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
284 <conditional name="regions_conditional">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
285 <param name="regions_option" value="disabled"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
286 </conditional>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
287 <section name="advanced_options">
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
288 <param name="vcf_stats_report" value="True"/>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
289 </section>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
290 <output name="vcf_file" ftype="vcf">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
291 <assert_contents>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
292 <has_text text="#CHROM"/>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
293 <has_text text="##fileformat=VCFv4.2"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
294 </assert_contents>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
295 </output>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
296 <output name="html_report" ftype="html">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
297 <assert_contents>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
298 <is_valid_xml />
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
299 <has_n_lines n="34" delta="10" />
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
300 <has_text text="Variant types"/>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
301 </assert_contents>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
302 </output>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
303 </test>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
304 <!-- Test indexed reference format input-->
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
305 <test expect_num_outputs="2">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
306 <conditional name="reference_genome">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
307 <param name="source" value="indexed"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
308 <param name="index" value="phix174"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
309 </conditional>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
310 <param name="reads" value="reads.bam"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
311 <param name="model_type" value="WGS"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
312 <conditional name="regions_conditional">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
313 <param name="regions_option" value="disabled"/>
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
314 </conditional>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
315 <section name="advanced_options">
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
316 <param name="vcf_stats_report" value="True"/>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
317 </section>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
318 <output name="vcf_file" ftype="vcf">
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
319 <assert_contents>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
320 <has_text text="#CHROM"/>
0
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
iuc
parents:
diff changeset
321 <has_text text="##fileformat=VCFv4.2"/>
4
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
322 </assert_contents>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
323 </output>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
324 <output name="html_report" ftype="html">
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
325 <assert_contents>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
326 <is_valid_xml />
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
327 <has_n_lines n="34" delta="10" />
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
328 <has_text text="Variant types"/>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
329 </assert_contents>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
330 </output>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
331 </test>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
332 <!-- Test runtime report output-->
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
333 <test expect_num_outputs="3">
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
334 <conditional name="reference_genome">
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
335 <param name="source" value="history"/>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
336 <param name="history_item" value="reference.fasta"/>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
337 </conditional>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
338 <param name="reads" value="reads.bam"/>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
339 <param name="model_type" value="WGS"/>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
340 <conditional name="regions_conditional">
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
341 <param name="regions_option" value="disabled"/>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
342 </conditional>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
343 <section name="advanced_options">
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
344 <param name="vcf_stats_report" value="True"/>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
345 <param name="create_runtime_report" value="True"/>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
346 </section>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
347 <output name="vcf_file" ftype="vcf">
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
348 <assert_contents>
09fff9439112 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/deepvariant commit bf3bb5d5a1f442208f054523fbcf0be4cc366a35
iuc
parents: 3
diff changeset
349 <has_text text="#CHROM"/>
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350 <has_text text="##fileformat=VCFv4.2"/>
0
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351 </assert_contents>
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352 </output>
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353 <output name="html_report" ftype="html">
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354 <assert_contents>
4
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355 <is_valid_xml />
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356 <has_n_lines n="34" delta="10" />
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357 <has_text text="Variant types"/>
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358 </assert_contents>
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359 </output>
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360 <output name="runtime_report" ftype="html">
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361 <assert_contents>
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362 <is_valid_xml />
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363 <has_text text="Overall runtime"/>
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364 </assert_contents>
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365 </output>
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366 </test>
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367 <!-- Test haploid contigs and PAR regions BED-->
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368 <test expect_num_outputs="2">
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369 <conditional name="reference_genome">
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370 <param name="source" value="history"/>
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371 <param name="history_item" value="reference.fasta"/>
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372 </conditional>
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373 <param name="reads" value="reads.bam"/>
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374 <param name="model_type" value="WGS"/>
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375 <conditional name="regions_conditional">
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376 <param name="regions_option" value="disabled"/>
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377 </conditional>
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378 <section name="advanced_options">
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379 <param name="vcf_stats_report" value="True"/>
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380 <param name="haploid_contigs" value="K03455"/>
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381 <param name="par_regions_bed" value="phix174.par_regions.bed" ftype="bed"/>
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382 </section>
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383 <output name="vcf_file" ftype="vcf">
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384 <assert_contents>
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385 <has_text text="#CHROM"/>
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386 <has_text text="##fileformat=VCFv4.2"/>
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387 </assert_contents>
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388 </output>
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389 <output name="html_report" ftype="html">
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390 <assert_contents>
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391 <is_valid_xml />
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392 <has_n_lines n="34" delta="10" />
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393 <has_text text="Variant types"/>
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394 </assert_contents>
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395 </output>
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396 </test>
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397 <!-- Test expert options extra-args-->
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398 <test expect_num_outputs="2">
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399 <conditional name="reference_genome">
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400 <param name="source" value="history"/>
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401 <param name="history_item" value="reference.fasta"/>
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402 </conditional>
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403 <param name="reads" value="reads.bam"/>
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404 <param name="model_type" value="WGS"/>
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405 <conditional name="regions_conditional">
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406 <param name="regions_option" value="disabled"/>
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407 </conditional>
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408 <section name="advanced_options">
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409 <param name="vcf_stats_report" value="True"/>
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410 </section>
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411 <section name="expert_options">
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412 <param name="make_examples_extra_args" value="min_base_quality=10"/>
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413 <param name="call_variants_extra_args" value="allow_empty_examples=true"/>
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414 <param name="postprocess_variants_extra_args" value="cnn_homref_call_min_gq=20.0"/>
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415 </section>
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416 <output name="vcf_file" ftype="vcf">
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417 <assert_contents>
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418 <has_text text="#CHROM"/>
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419 <has_text text="##fileformat=VCFv4.2"/>
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420 </assert_contents>
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421 </output>
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422 <output name="html_report" ftype="html">
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423 <assert_contents>
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424 <is_valid_xml />
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425 <has_n_lines n="34" delta="10" />
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426 <has_text text="Variant types"/>
0
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427 </assert_contents>
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428 </output>
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429 </test>
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430 </tests>
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431 <help><![CDATA[
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432
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
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433 .. class:: infomark
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434
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
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435 **Purpose**
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436
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
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437 DeepVariant is a deep learning-based variant caller that takes aligned reads (in BAM or CRAM format), produces pileup image tensors from them, classifies each tensor using a convolutional neural network, and finally reports the results in a standard VCF or gVCF file.
2036bbf304d6 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deepvariant commit e46feb5432b28a9360a1d4e8a6618e6ed91008fe"
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438
4
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439 DeepVariant runs a pipeline of three steps: **make_examples**, **call_variants**, and **postprocess_variants**. The **Expert options** section exposes extra-args fields for each step. Use these only with parameters that are accepted by the corresponding DeepVariant binary (see `run_deepvariant --helpfull` and the subcommand help, e.g. `/opt/deepvariant/bin/make_examples --helpfull` in the container).
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440
0
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441 DeepVariant supports germline variant-calling in diploid organisms.
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442
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443 - NGS (Illumina) data for either a `whole genome <https://github.com/google/deepvariant/blob/r1.9/docs/deepvariant-case-study.md>`_ or `whole exome <https://github.com/google/deepvariant/blob/r1.9/docs/deepvariant-exome-case-study.md>`_.
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444 - PacBio HiFi data, see the `PacBio case study <https://github.com/google/deepvariant/blob/r1.9/docs/deepvariant-pacbio-model-case-study.md>`_.
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445 - Hybrid PacBio HiFi + Illumina WGS, see the `hybrid case study <https://github.com/google/deepvariant/blob/r1.9/docs/deepvariant-hybrid-case-study.md>`_.
0
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446
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447 Please also note:
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448
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449 For somatic data or any other samples where the genotypes go beyond two copies of DNA, DeepVariant will not work out of the box because the only genotypes supported are hom-alt, het, and hom-ref.
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450
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451 The models included with DeepVariant are only trained on human data. For other organisms, see the blog post on `non-human variant-calling <https://google.github.io/deepvariant/posts/2018-12-05-improved-non-human-variant-calling-using-species-specific-deepvariant-models/>`_ for some possible pitfalls and how to handle them.
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452
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453 ----
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454
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455 .. class:: infomark
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456
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457 **How DeepVariants works**
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458
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459 DeepVariant relies on `Nucleus <https://github.com/google/nucleus>`_, a library of Python and C++ code for reading and writing data in common genomics file formats (like SAM and VCF) designed for painless integration with the `TensorFlow <https://www.tensorflow.org/>`_ machine learning framework. Nucleus was built with DeepVariant in mind and open-sourced separately so it can be used by anyone in the genomics research community for other projects. See this blog post on `Using Nucleus and TensorFlow for DNA Sequencing Error Correction <https://google.github.io/deepvariant/posts/2019-01-31-using-nucleus-and-tensorflow-for-dna-sequencing-error-correction/>`_.
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460
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461
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462 ]]> </help>
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463 <expand macro="citations"/>
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464 </tool>