Mercurial > repos > iuc > cd_hit
annotate cd_hit.xml @ 0:48eda4e4df5d draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
| author | iuc |
|---|---|
| date | Mon, 15 Oct 2018 10:54:30 -0400 |
| parents | |
| children | baa8e104a7d6 |
| rev | line source |
|---|---|
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0
48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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1 <tool id="cd_hit" name="cd-hit" version="4.6.8.1"> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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2 <description>Cluster or compare biological sequence datasets</description> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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3 <requirements> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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4 <requirement type="package" version="4.6.8">cd-hit</requirement> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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5 </requirements> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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6 <version_command><![CDATA[ |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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7 cd-hit | grep "CD-HIT version" | cut -d" " -f 4 |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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8 ]]></version_command> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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9 <command detect_errors="exit_code"><![CDATA[ |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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10 cd-hit$est.est_select$twod.twod_select |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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11 -i '$fasta_in' |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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12 -o rep_seq |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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13 -c $est.similarity |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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14 -n $est.wordsize |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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15 #if $est.est_select == '-est': |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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16 $est.strand |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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17 #if str($est.estadvalign.mask) != 'None': |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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18 -mask '$est.estadvalign.mask' |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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19 #end if |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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20 -match $est.estadvalign.match |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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21 -mismatch $est.estadvalign.mismatch |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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22 -gap $est.estadvalign.gap |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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23 -gap-ext $est.estadvalign.gapext |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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24 #else: |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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25 -t $est.redtol |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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26 #end if |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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27 #if $twod.twod_select == '-2d': |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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28 -i2 '$fasta_in2' |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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29 #if $advanced.advalign.style == 'local': |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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30 -s2 $advanced.advalign.advancedtwod.cutoff_diff_len2 |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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31 -S2 $advanced.advalign.advancedtwod.aa_cutoff_diff_len2 |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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32 #end if |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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33 #end if |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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34 |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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35 -b $advanced.band_width |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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36 -l $advanced.throw_away_len |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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37 #if $advanced.advalign.style == 'local': |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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38 -G 0 |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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39 -aL $advanced.advalign.align_coverage_long |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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40 -AL $advanced.advalign.align_coverage_long_control |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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41 -aS $advanced.advalign.align_coverage_short |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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42 -AS $advanced.advalign.align_coverage_short_control |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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43 -A $advanced.advalign.align_coverage_min |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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44 -s $advanced.advalign.cutoff_diff_len |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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45 -S $advanced.advalign.aa_cutoff_diff_len |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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46 #end if |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
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47 -uL $advanced.max_unmatched_per_l |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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48 -uS $advanced.max_unmatched_per_s |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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changeset
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49 -U $advanced.max_unmatched_len |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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changeset
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50 $advanced.accurate |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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51 $advanced.inram |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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changeset
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52 #if $print_alnovl.print_alnovl_select == "yes": |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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53 -p 1 |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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54 -d $print_alnovl.desclen |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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55 #end if |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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56 |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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57 ## instead of 800 (default) we use 0:unlimited |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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58 -M \${GALAXY_MEMORY_MB:-0} |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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59 -T \${GALAXY_SLOTS:-1} |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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60 ]]></command> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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61 <inputs> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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62 <param name="fasta_in" argument="-i" type="data" format="fasta" label="Sequences to cluster/compare"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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63 <conditional name="twod"> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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64 <param name="twod_select" type="select" label="Cluster / Compare (i.e. call cd-hit[-est] / cd-hit[-est]-2d)?"> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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65 <option value="" selected="true">Cluster sequences</option> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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66 <option value="-2d">Compare with 2nd sequence data set</option> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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67 </param> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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68 <when value=""/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
changeset
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69 <when value="-2d"> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
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70 <param name="fasta_in2" argument="-i2" type="data" format="fasta" label="Other sequences to cluster/compare"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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71 </when> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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72 </conditional> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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73 <conditional name="est"> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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74 <param name="est_select" type="select" label="Sequence type?" help="For nucleotides the -est variant of cd-hit is called"> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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75 <option value="" selected="true">Protein</option> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
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76 <option value="-est">Nucleotides</option> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
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77 </param> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
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78 <when value=""> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
parents:
diff
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79 <param name="similarity" argument="-c" type="float" min="0.4" max="1.0" value="0.9" label="Sequence identity threshold" help="Global sequence identity: number of identical alignment positions divided by the full length of the shorter sequence"/> |
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48eda4e4df5d
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80 <param name="wordsize" argument="-n" type="integer" min="2" max="5" value="5" label="Word size"> |
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48eda4e4df5d
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81 <help>Suggested word size: |
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48eda4e4df5d
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82 5 for thresholds 0.7 ~ 1.0 |
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83 4 for thresholds 0.6 ~ 0.7 |
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48eda4e4df5d
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84 3 for thresholds 0.5 ~ 0.6 |
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48eda4e4df5d
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85 2 for thresholds 0.4 ~ 0.5 (-n) |
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48eda4e4df5d
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86 </help> |
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87 </param> |
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48eda4e4df5d
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88 <param name="redtol" argument="-t" type="integer" value="2" label="Tolerance for redundance"/> |
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48eda4e4df5d
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89 </when> |
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90 <when value="-est"> |
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91 <param name="similarity" argument="-c" type="float" min="0.8" max="1.0" value="0.9" label="Sequence identity threshold" help="Global sequence identity: number of identical alignment positions divided by the full length of the shorter sequence"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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92 <param name="wordsize" argument="-n" type="integer" min="4" max="11" value="10" label="Word size"> |
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48eda4e4df5d
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93 <help>Suggested word size: |
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48eda4e4df5d
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94 10,11 for threshold in 0.95 ~ 1.0 |
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48eda4e4df5d
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95 8,9 for threshold in 0.9 ~ 0,95 |
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48eda4e4df5d
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96 7 for threshold in 0.88 ~ 0.9 |
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48eda4e4df5d
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97 6 for threshold in 0.85 ~ 0.88 |
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98 5 for threshold in 0.80 ~ 0.85 |
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99 4 for threshold in 0.75 ~ 0.8 (-n) |
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48eda4e4df5d
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100 </help> |
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101 </param> |
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102 <param name="strand" argument="-r" type="boolean" truevalue="-r 1" falsevalue="-r 0" checked="false" label="Compare both strands?"/> |
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48eda4e4df5d
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103 <section name="estadvalign" title="Advanced EST alignment options"> |
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48eda4e4df5d
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104 <param argument="-mask" type="text" optional="true" label="Masking letters" help="NX, to mask out both 'N' and 'X'"/> |
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48eda4e4df5d
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105 <param argument="-match" type="integer" value="2" label="Match score" help="Default 2 (1 for T-U and N-N)"/> |
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48eda4e4df5d
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106 <param argument="-mismatch" type="integer" value="-2" label="Mismatch score"/> |
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107 <param argument="-gap" type="integer" value="-6" label="Gap opening score"/> |
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48eda4e4df5d
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108 <param name="gapext" argument="-gap-ext" type="integer" value="-1" label="Gap extension score"/> |
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109 </section> |
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110 </when> |
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111 </conditional> |
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112 <section name="advanced" title="Advanced options"> |
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113 <param name="band_width" argument="-b" type="integer" min="1" value="20" label="Alignment band width"/> |
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114 <param name="throw_away_len" argument="-l" type="integer" min="1" value="10" label="Length of throw away sequences"/> |
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115 <conditional name="advalign"> |
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116 <param name="style" type="select" label="Use local/global sequence identity" help="Local sequence identity: number of identical amino alignment positions divided by the length of the alignment. Note: in local mode one of -aL, aS, or A must be > 0!"> |
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117 <option value="global" selected="true">Global</option> |
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118 <option value="local" >Local</option> |
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119 </param> |
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120 <when value="global"/> |
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121 <when value="local"> |
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122 <param name="align_coverage_long" argument="-aL" type="float" min="0.0" max="1.0" value="0.0" label="Alignment coverage for the longer sequence" help="Fraction of the longer sequence that must be covered"/> |
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48eda4e4df5d
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123 <param name="align_coverage_long_control" argument="-AL" type="integer" min="0" value="99999999" label="Alignment coverage control for the longer sequence " help="Maximum number of residues uncovered residues of the longer sequence"/> |
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124 <param name="align_coverage_short" argument="-aS" type="float" min="0.0" max="1.0" value="0.0" label="Alignment coverage for the shorter sequence" help="Fraction of the shorter sequence that must be covered"/> |
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48eda4e4df5d
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125 <param name="align_coverage_short_control" argument="-AS" type="integer" min="0" value="99999999" label="Alignment coverage control for the shorter sequence" help="Maximum number of residues uncovered residues of the shorter sequence"/> |
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126 <param name="align_coverage_min" argument="-A" type="integer" min="0" value="0" label="Minimal alignment coverage control for the both sequences" help="Minimum number of residues of both sequences that must be covered"/> |
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127 <param name="cutoff_diff_len" argument="-s" type="float" min="0.0" max="1.0" value="0.0" label="Length difference cutoff" help="If set to 0.9, the shorter sequences need to be at least 90% length of the representative of the cluster"/> |
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48eda4e4df5d
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128 <param name="aa_cutoff_diff_len" argument="-S" type="integer" min="0" value="999999" label="Length difference cutoff in residues" help="If set to 60, the length difference between the shorter sequences and the representative of the cluster can not be bigger than 60"/> |
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129 <section name="advancedtwod" title="Advanced options for 2D local alignment"> |
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130 <param name="cutoff_diff_len2" argument="-s2" type="float" min="0.0" max="1.0" value="1.0" label="Length difference cutoff for other sequences" help="By default, seqs in db1 >= seqs in db2 in a same cluster if set to 0.9, seqs in db1 may just >= 90% seqs in db2. Only effective in local alignment mode"/> |
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131 <param name="aa_cutoff_diff_len2" argument="-S2" type="integer" min="0" value="0" label="Length difference cutoff in residues for other sequences" help="by default, seqs in db1 >= seqs in db2 in a same cluster if set to 60, seqs in db2 may 60aa longer than seqs in db1. Only effective in local alignment mode"/> |
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48eda4e4df5d
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132 </section> |
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133 </when> |
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134 </conditional> |
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135 <param name="max_unmatched_per_l" argument="-uL" type="float" min="0.0" max="1.0" value="1.0" label="Maximum unmatched percentage for the shorter sequence" help="If set to 0.1, the unmatched region (excluding leading and tailing gaps) must not be more than 10% of the sequence"/> |
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136 <param name="max_unmatched_per_s" argument="-uS" type="float" min="0.0" max="1.0" value="1.0" label="Maximum unmatched percentage for the shorter sequence" help="If set to 0.1, the unmatched region (excluding leading and tailing gaps) must not be more than 10% of the sequence"/> |
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48eda4e4df5d
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137 <param name="max_unmatched_len" argument="-U" type="integer" min="0" value="99999999" label="Maximum unmatched length" help="If set to 10, the unmatched region (excluding leading and tailing gaps) must not be more than 10 bases"/> |
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138 <param name="inram" argument="-B" type="boolean" truevalue="-B 0" falsevalue="-B 1" checked="true" label="Sequences are stored in RAM" help="If false: sequence are stored on hard drive - use for huge data sets"/> |
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139 <param name="accurate" argument="-g" type="boolean" truevalue="-g 1" falsevalue="-g 0" checked="false" label="Accurate but slow mode" help="By cd-hit's default algorithm, a sequence is clustered to the first cluster that meet the threshold (fast cluster). If set to true, the program will cluster it into the most similar cluster that meet the threshold (accurate but slow mode)"/> |
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140 </section> |
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141 |
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142 <conditional name="print_alnovl"> |
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143 <param name="print_alnovl_select" type="select" label="Print alignment overlap in .clstr file?"> |
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144 <option value="no" selected="true">No</option> |
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145 <option value="yes">Yes</option> |
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146 </param> |
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147 <when value="no"/> |
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148 <when value="yes"> |
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149 <param name="desclen" argument="-d" type="integer" min="0" value="20" label="Length of description in .clstr file" help="If set to 0, it takes the fasta defline and stops at first space"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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150 </when> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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diff
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151 </conditional> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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152 </inputs> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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153 |
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48eda4e4df5d
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154 <outputs> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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155 <data name="clusters_out" format="txt" label="${tool.name} on ${on_string}: Clusters" from_work_dir="rep_seq.clstr"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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156 <data name="fasta_out" format="fasta" label="${tool.name} on ${on_string}: Representative sequences" from_work_dir="rep_seq"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
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157 </outputs> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
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158 |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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159 <tests> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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160 <!--cd-hit with default options --> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
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161 <test> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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162 <param name="fasta_in" value="cd_hit_protein_in.fasta" /> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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163 <conditional name="twod"> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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164 <param name="twod_select" value="" /> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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165 </conditional> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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166 <conditional name="est"> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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167 <param name="est_select" value="" /> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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168 <param name="wordsize" value="5" /> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
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169 </conditional> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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170 <param name="similarity" value="0.9" /> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
iuc
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171 <output name="clusters_out" file="protein_clusters_output.txt"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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172 <output name="fasta_out" file="protein_fasta_output.fasta"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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173 </test> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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174 <!--cd-hit local (default options), changed similarity, and output string length --> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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175 <test> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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176 <param name="fasta_in" value="cd_hit_protein_in.fasta" /> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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177 <conditional name="twod"> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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178 <param name="twod_select" value="" /> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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179 </conditional> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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180 <conditional name="est"> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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181 <param name="est_select" value="" /> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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182 <param name="wordsize" value="5" /> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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183 </conditional> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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184 <param name="similarity" value="0.8" /> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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185 <section name="advanced"> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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186 <conditional name="advalign"> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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187 <param name="style" value="local" /> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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188 <param name="align_coverage_short" value="0.9" /> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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189 </conditional> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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190 </section> |
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48eda4e4df5d
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191 <conditional name="print_alnovl"> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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192 <param name="print_alnovl_select" value="yes"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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193 <param name="desclen" value="40"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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194 </conditional> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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195 <output name="clusters_out" file="protein_clusters_output_local.txt"/> |
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48eda4e4df5d
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196 <output name="fasta_out" file="protein_fasta_output_local.fasta"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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197 </test> |
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48eda4e4df5d
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198 <!-- cd-hit-est --> |
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48eda4e4df5d
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199 <test> |
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48eda4e4df5d
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200 <param name="fasta_in" value="cd_hit_est_in.fa" /> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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201 <conditional name="twod"> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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202 <param name="twod_select" value="" /> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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203 </conditional> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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204 <conditional name="est"> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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205 <param name="est_select" value="-est" /> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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206 <param name="wordsize" value="8"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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207 <param name="strand" value="false"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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208 </conditional> |
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48eda4e4df5d
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209 <param name="similarity" value="0.9"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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210 <output name="clusters_out" file="est_clusters_output.txt"/> |
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48eda4e4df5d
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211 <output name="fasta_out" file="est_fasta_output.fasta"/> |
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48eda4e4df5d
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212 </test> |
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48eda4e4df5d
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213 <!-- cd-hit-est-2d (also changing strand param) --> |
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48eda4e4df5d
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214 <test> |
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48eda4e4df5d
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215 <param name="fasta_in" value="db1.fasta" /> |
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48eda4e4df5d
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216 <conditional name="twod"> |
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48eda4e4df5d
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217 <param name="twod_select" value="-2d" /> |
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48eda4e4df5d
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218 <param name="fasta_in2" value="db2.fasta" /> |
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48eda4e4df5d
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219 </conditional> |
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48eda4e4df5d
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220 <conditional name="est"> |
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48eda4e4df5d
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221 <param name="est_select" value="-est" /> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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222 <param name="wordsize" value="8"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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223 <param name="strand" value="true"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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224 </conditional> |
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48eda4e4df5d
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225 <param name="similarity" value="0.9"/> |
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48eda4e4df5d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cdhit commit 8e14fc2573a53eaf8a538e018ae292f4c3134ec2
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226 <output name="clusters_out" file="est-2d.txt.clstr"/> |
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48eda4e4df5d
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227 <output name="fasta_out" file="est-2d.txt"/> |
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48eda4e4df5d
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228 </test> |
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48eda4e4df5d
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229 </tests> |
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48eda4e4df5d
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iuc
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230 <help><![CDATA[ |
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231 **What it does** |
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232 |
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233 cd-hit stands for Cluster Database at High Identity with Tolerance. The tool implements four variants: cd-hit, cd-hit-est, cd-hit-2d, and cd-hit-est-2d. |
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234 |
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235 The program cd-hit (resp. cd-hit-est) takes a FASTA format aminoacid (resp. nucleotide) sequence database as input and produces a set of 'non-redundant' (nr) representative sequences as output. In addition cd-hit outputs a cluster file, documenting the members of the sequence clusters for each nr sequence representative. The idea is to reduce the overall size of the database without removing any sequence information by only removing 'redundant' (or highly similar) sequences. This is why the resulting database is called non-redundant (nr). Essentially, cd-hit (resp. cd-hit-est) produces a set of closely related protein (resp. nucleotide sequence) families from a given FASTA sequence database. |
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236 |
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237 The program cd-hit-2d (resp. cd-hit-est-2d) compares two aminoacid (resp. nucleotide) sequence datasets (db1, db2) in FASTA format. It identifies the sequences in db2 that are similar to db1 at a certain threshold. It outputs two files: a FASTA file of sequences in db2 that are not similar to db1 and a text file that lists similar sequences between db1 & db2. |
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238 |
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239 .. _CD-HIT: http://weizhongli-lab.org/cd-hit/ |
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240 |
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241 ------ |
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242 |
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243 **Inputs** |
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244 |
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245 cd-hit/cd-hit-2d requires a (two) protein FASTA file(s) as input. |
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246 |
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247 cd-hit-est/cd-hit-est-2d requires a (two) nucleotide FASTA file(s) as input. |
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248 |
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249 ------ |
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250 |
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251 **Outputs** |
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252 |
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253 For cd-hit and cd-hit-est: |
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254 |
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255 1. The first output is a FASTA file containing representative sequences. |
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256 |
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257 2. The second output is a text file listing the mapping of sequences to the representative sequences: |
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258 |
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259 >Cluster 0 |
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260 0 2799aa, >PF04998.6|RPOC2_CHLRE/275-3073... * |
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261 >Cluster 1 |
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262 0 2214aa, >PF06317.1|Q6Y625_9VIRU/1-2214... at 80% |
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263 1 2215aa, >PF06317.1|O09705_9VIRU/1-2215... at 84% |
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264 2 2217aa, >PF06317.1|Q6Y630_9VIRU/1-2217... * |
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265 3 2216aa, >PF06317.1|Q6GWS6_9VIRU/1-2216... at 84% |
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266 4 527aa, >PF06317.1|Q67E14_9VIRU/6-532... at 63% |
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267 >Cluster 2 |
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268 0 2202aa, >PF06317.1|Q6UY61_9VIRU/8-2209... at 60% |
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269 1 2208aa, >PF06317.1|Q6IVU4_JUNIN/1-2208... * |
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270 2 2207aa, >PF06317.1|Q6IVU0_MACHU/1-2207... at 73% |
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271 3 2208aa, >PF06317.1|RRPO_TACV/1-2208... at 69% |
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272 |
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273 For cd-hit-2d and cd-hit-est-2d: |
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274 |
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275 1. The first output is a FASTA file of sequences in db2 that are not similar to db1. |
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276 |
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277 2. The second output is a text file that lists similar sequences between db1 & db2 |
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278 ]]></help> |
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279 <citations> |
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280 <citation type="doi">10.1093/bioinformatics/btl158</citation> |
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281 <citation type="doi">10.1093/bioinformatics/bts565</citation> |
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282 </citations> |
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283 </tool> |
