Mercurial > repos > iuc > bio_hansel
annotate bio_hansel.xml @ 0:726cd66ca2f9 draft
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
| author | iuc |
|---|---|
| date | Tue, 03 Oct 2017 10:38:47 -0400 |
| parents | |
| children | ddae7615b431 |
| rev | line source |
|---|---|
|
0
726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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1 <tool id="bio_hansel" name="Salmonella Subtyping" version="0.1.0"> |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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2 <requirements> |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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3 <requirement type="package" version="0.1.0">bio_hansel</requirement> |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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4 <requirement type="package" version="17.2.0">attrs</requirement> |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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5 </requirements> |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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6 <command detect_errors="exit_code"><![CDATA[ |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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7 ## Preparing file input. |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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8 #if $data_type.type == "paired": |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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9 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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10 ln -s '$data_type.fastq_input1' fast_q_paired_1.fastq && |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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11 ln -s '$data_type.fastq_input2' fast_q_paired_2.fastq && |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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12 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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13 #elif $data_type.type == "collection": |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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14 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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15 ln -s '$data_type.fastq_input1.forward' fast_q_paired_1.fastq && |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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16 ln -s '$data_type.fastq_input1.reverse' fast_q_paired_2.fastq && |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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17 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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18 #elif $data_type.type == "single": |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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19 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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20 #if $data_type.fastq_input1.is_of_type('fastqsanger') or $data_type.fastq_input1.is_of_type('fastq'): |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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21 ln -s '$data_type.fastq_input1' fast_q_single.fastq && |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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22 #end if |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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23 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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24 #if $data_type.fastq_input1.is_of_type('fasta'): |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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25 ln -s '$data_type.fastq_input1' fast_a_single.fasta && |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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26 #end if |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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27 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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28 #end if |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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29 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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30 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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31 ## Checking for custom scheme. |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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32 #if $type_of_scheme.scheme_type == "custom": |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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33 #if $type_of_scheme.scheme_input.is_of_type('fasta'): |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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34 ln -s '$type_of_scheme.scheme_input' custom_scheme.fasta && |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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35 #end if |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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36 #end if |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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37 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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38 ## Start the actual command here |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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39 hansel |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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40 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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41 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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42 ## Select the scheme |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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43 -s |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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44 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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45 #if $type_of_scheme.scheme_type == "heidelberg": |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
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changeset
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46 heidelberg |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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47 #elif $type_of_scheme.scheme_type == "enteritidis": |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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48 enteritidis |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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49 #elif $type_of_scheme.scheme_type == "custom": |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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50 custom_scheme.fasta |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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51 #end if |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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52 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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53 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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54 #if $kmer_min |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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55 --min-kmer-freq $kmer_min |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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56 #end if |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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57 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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58 #if $kmer_max |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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59 --max-kmer-freq $kmer_max |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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60 #end if |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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61 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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62 ## Adding more parameters to the command. |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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63 -vvv -t "\${GALAXY_SLOTS:-1}" -o results.tab -O match_results.tab |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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64 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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65 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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66 ## Entering the file inputs. |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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67 #if $data_type.type == "single": |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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68 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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69 #if $data_type.fastq_input1.is_of_type('fastqsanger') or $data_type.fastq_input1.is_of_type('fastq'): |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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70 fast_q_single.fastq |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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71 #end if |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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72 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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73 #if $data_type.fastq_input1.is_of_type('fasta'): |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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74 fast_a_single.fasta |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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75 #end if |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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76 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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77 #else |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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78 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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79 ##use -p to declare using two files. |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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80 -p fast_q_paired_1.fastq fast_q_paired_2.fastq |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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81 |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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82 #end if |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
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83 ]]></command> |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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84 <inputs> |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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85 <conditional name="data_type"> |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
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86 <param name="type" type="select" label="Specify the read type."> |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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87 <option value="single">Single-end Data</option> |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
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88 <option value="paired">Paired-end Data</option> |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
diff
changeset
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89 <option value="collection">Collection Paired-end Data</option> |
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726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
iuc
parents:
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90 </param> |
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91 <when value="single"> |
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92 <param name="fastq_input1" type="data" format="fastqsanger, fastq, fasta" label="Single end read file(s)"/> |
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93 </when> |
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94 <when value="paired"> |
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95 <param name="fastq_input1" type="data" format="fastqsanger, fastq" label="Forward paired-end read file"/> |
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96 <param name="fastq_input2" type="data" format="fastqsanger, fastq" label="Reverse paired-end read file"/> |
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97 </when> |
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98 <when value="collection"> |
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99 <param name="fastq_input1" type="data_collection" label="Paired-end reads collection" optional="false" format="fastqsanger, fastq" collection_type="paired" /> |
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100 </when> |
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101 </conditional> |
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102 <conditional name="type_of_scheme"> |
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103 <param name="scheme_type" type="select" label="Specify scheme to use. (Heidelberg is default)"> |
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104 <option value="heidelberg">Heidelberg scheme</option> |
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105 <option value="enteritidis">Enteritidis scheme</option> |
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106 <option value="custom">Specify your own custom scheme</option> |
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107 </param> |
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108 <when value="heidelberg"/> |
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109 <when value="enteritidis"/> |
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110 <when value="custom"> |
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111 <param name="scheme_input" type="data" format="fasta" label="Scheme Input"/> |
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112 </when> |
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113 </conditional> |
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114 <param name="kmer_min" argument="--min-kmer-freq" optional="True" type="integer" min="0" label="Min k-mer freq/coverage" help="default = 10"/> |
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115 <param name="kmer_max" argument="--max-kmer-freq" optional="True" type="integer" min="1" label="Max k-mer freq/coverage" help="default = 200"/> |
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116 </inputs> |
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117 <outputs> |
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118 <data format="tabular" name="results.tab" from_work_dir="results.tab" label="results.tab"/> |
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119 <data format="tabular" name="match_results.tab" from_work_dir="match_results.tab" label="match_results.tab"/> |
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120 </outputs> |
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121 <tests> |
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122 <test> |
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123 <param name="type" value="single"/> |
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124 <param name="type_of_scheme" value="heidelberg"/> |
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125 <param name="fastq_input1" value="SRR1002850_SMALL.fasta"/> |
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126 <output name="results.tab"> |
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127 <assert_contents> |
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128 <!-- Verifying that the columns are as expected. --> |
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129 <has_text_matching expression="sample\s+scheme\s+subtype\s+all_subtypes\s+tiles_matching_subtype\s+are_subtypes_consistent\s+inconsistent_subtypes\s+n_tiles_matching_all\s+n_tiles_matching_all_total\s+n_tiles_matching_positive\s+n_tiles_matching_positive_total\s+n_tiles_matching_subtype\s+n_tiles_matching_subtype_total\s+file_path"/> |
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130 <!-- Verifying that the output of running the test file is expected. This is done via REGEX because the name and path of the file outputted to results.tab changes each test. --> |
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131 <has_text_matching expression="(heidelberg)\s+(2.2.2.2.1.4)\s+(2;)\s+(2.2;)\s+(2.2.2;)\s+(2.2.2.2;)\s+(2.2.2.2.1;)\s+(2.2.2.2.1.4)\s+(1037658-2.2.2.2.1.4;)\s+(2154958-2.2.2.2.1.4;)\s+(3785187-2.2.2.2.1.4)\s+(True)\s+(202)\s+(202)\s+(17)\s+(17)\s+(3)\s+(3)"/> |
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132 </assert_contents> |
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133 </output> |
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134 <output name="match_results.tab"> |
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135 <assert_contents> |
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136 <!-- This is the last line in the file, this assertion is to make sure that we have the correct number of items. --> |
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137 <has_text_matching expression="negative4738855-1.1"/> |
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138 </assert_contents> |
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139 </output> |
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140 </test> |
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141 <test> |
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142 <param name="type" value="paired"/> |
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143 <param name="type_of_scheme" value="heidelberg"/> |
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144 <param name="fastq_input1" value="SRR5646583_SMALL_1.fastq"/> |
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145 <param name="fastq_input2" value="SRR5646583_SMALL_2.fastq"/> |
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146 <output name="results.tab"> |
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147 <assert_contents> |
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148 <!-- Verifying that the columns are as expected. --> |
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149 <has_text_matching expression="sample\s+scheme\s+subtype\s+all_subtypes\s+tiles_matching_subtype\s+are_subtypes_consistent\s+inconsistent_subtypes\s+n_tiles_matching_all\s+n_tiles_matching_all_total\s+n_tiles_matching_positive\s+n_tiles_matching_positive_total\s+n_tiles_matching_subtype\s+n_tiles_matching_subtype_total\s+file_path"/> |
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150 <!-- Verifying that the output of running the test file is expected. This is done via REGEX because the name and path of the file outputted to results.tab changes each test. --> |
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151 <has_text_matching expression="(heidelberg)\s+(2.2.1.1.1.1)\s+(2;)\s+(2.2;)\s+(2.2.1;)\s+(2.2.1.1;)\s+(2.2.1.1.1;)\s+(2.2.1.1.1.1)\s+(1983064-2.2.1.1.1.1;)\s+(4211912-2.2.1.1.1.1)\s+(True)\s+(202)\s+(202)\s+(20)\s+(20)\s+(2)\s+(2)"/> |
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152 </assert_contents> |
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153 </output> |
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154 </test> |
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155 </tests> |
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156 <help><![CDATA[ |
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157 *********************************************************** |
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158 bio_hansel - Heidelberg And eNteritidis Snp ELucidation |
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159 *********************************************************** |
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160 |
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161 Subtype *Salmonella enterica* subsp. enterica serovar Heidelberg and Enteritidis genomes using *in-silico* 33 bp k-mer SNP subtyping schemes developed by Genevieve Labbe et al. |
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162 Subtype *Salmonella* genome assemblies (FASTA files) and/or whole-genome sequencing reads (FASTQ files)! |
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163 |
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164 |
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165 Usage |
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166 ===== |
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167 1) Enter your FASTA/FASTQ file(s) |
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168 2) Select which scheme you would like to use (e.g. heidelberg, enteritidis, or specify your own) |
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169 3) Click Execute |
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170 |
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171 For more information visit `https://github.com/phac-nml/bio_hansel` |
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172 |
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planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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173 |
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planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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174 Example Usage |
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planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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175 ============= |
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planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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176 |
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177 Analysis of a single FASTA file |
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178 ------------------------------- |
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179 |
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180 Contents of ``results.tab``: |
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planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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181 |
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182 +------------+------------+-------------+------------------------------------------------+---------------------------------------------------------------+-------------------------+-----------------------+----------------------+----------------------------+---------------------------+---------------------------------+--------------------------+--------------------------------+------------+ |
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planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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183 | sample | scheme | subtype | all_subtypes | tiles_matching_subtype | are_subtypes_consistent | inconsistent_subtypes | n_tiles_matching_all | n_tiles_matching_all_total | n_tiles_matching_positive | n_tiles_matching_positive_total | n_tiles_matching_subtype | n_tiles_matching_subtype_total | file_path | |
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planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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184 +------------+------------+-------------+------------------------------------------------+---------------------------------------------------------------+-------------------------+-----------------------+----------------------+----------------------------+---------------------------+---------------------------------+--------------------------+--------------------------------+------------+ |
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planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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185 | file.fasta | heidelberg | 2.2.2.2.1.4 | 2; 2.2; 2.2.2; 2.2.2.2; 2.2.2.2.1; 2.2.2.2.1.4 | 1037658-2.2.2.2.1.4; 2154958-2.2.2.2.1.4; 3785187-2.2.2.2.1.4 | True | | 202 | 202 | 17 | 17 | 3 | 3 | file.fasta | |
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planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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186 +------------+------------+-------------+------------------------------------------------+---------------------------------------------------------------+-------------------------+-----------------------+----------------------+----------------------------+---------------------------+---------------------------------+--------------------------+--------------------------------+------------+ |
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187 |
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188 |
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189 |
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190 Contents of ``match_results.tab``: |
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191 |
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192 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+ |
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193 | tilename | stitle | pident | length | mismatch | gapopen | qstart | qend | sstart | send | evalue | bitscore | qlen | slen | seq | coverage | is_trunc | refposition | subtype | is_pos_tile | sample | file_path | scheme | |
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planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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194 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+ |
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195 | 775920-2.2.2.2 | NODE_2_length_512016_cov_46.4737_ID_3 | 100 | 33 | 0 | 0 | 1 | 33 | 474875 | 474907 | 2E-11 | 62.1 | 33 | 512016 | GTTCAGGTGCTACCGAGGATCGTTTTTGGTGCG | 1 | False | 775920 | 2.2.2.2 | True | out | file.fasta | heidelberg | |
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planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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196 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+ |
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197 | negative3305400-2.1.1.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 276235 | 276267 | 2E-11 | 62.1 | 33 | 427905 | CATCGTGAAGCAGAACAGACGCGCATTCTTGCT | 1 | False | negative3305400 | 2.1.1.1 | False | out | file.fasta | heidelberg | |
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planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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198 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+ |
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199 | negative3200083-2.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 170918 | 170950 | 2E-11 | 62.1 | 33 | 427905 | ACCCGGTCTACCGCAAAATGGAAAGCGATATGC | 1 | False | negative3200083 | 2.1 | False | out | file.fasta | heidelberg | |
|
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planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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200 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+ |
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201 | negative3204925-2.2.3.1.5 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 175760 | 175792 | 2E-11 | 62.1 | 33 | 427905 | CTCGCTGGCAAGCAGTGCGGGTACTATCGGCGG | 1 | False | negative3204925 | 2.2.3.1.5 | False | out | file.fasta | heidelberg | |
|
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planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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202 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+ |
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203 | negative3230678-2.2.2.1.1.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 201513 | 201545 | 2E-11 | 62.1 | 33 | 427905 | AGCGGTGCGCCAAACCACCCGGAATGATGAGTG | 1 | False | negative3230678 | 2.2.2.1.1.1 | False | out | file.fasta | heidelberg | |
|
726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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204 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+ |
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205 | negative3233869-2.1.1.1.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 204704 | 204736 | 2E-11 | 62.1 | 33 | 427905 | CAGCGCTGGTATGTGGCTGCACCATCGTCATTA | 1 | False | negative3233869 | 2.1.1.1.1 | False | out | file.fasta | heidelberg | |
|
726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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206 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+ |
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207 | negative3254229-2.2.3.1.3 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 225064 | 225096 | 2E-11 | 62.1 | 33 | 427905 | CGCCACCACGCGGTTAGCGTCACGCTGACATTC | 1 | False | negative3254229 | 2.2.3.1.3 | False | out | file.fasta | heidelberg | |
|
726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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208 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+ |
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209 | negative3257074-2.2.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 227909 | 227941 | 2E-11 | 62.1 | 33 | 427905 | CGGCAACCAGACCGACTACGCCGCCAAGCAGAC | 1 | False | negative3257074 | 2.2.1 | False | out | file.fasta | heidelberg | |
|
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210 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+ |
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211 | negative3264474-2.2.2.1.1.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 235309 | 235341 | 2E-11 | 62.1 | 33 | 427905 | AATGGCGCCGATCGTCGCCAGATAACCGTTGCC | 1 | False | negative3264474 | 2.2.2.1.1.1 | False | out | file.fasta | heidelberg | |
|
726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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212 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+ |
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213 | negative3267927-2.2.2.2.2.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 238762 | 238794 | 2E-11 | 62.1 | 33 | 427905 | AAAGAGAAATATGATGCCAGGCTGATACATGAC | 1 | False | negative3267927 | 2.2.2.2.2.1 | False | out | file.fasta | heidelberg | |
|
726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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214 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+ |
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215 | negative3278067-1.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 248902 | 248934 | 2E-11 | 62.1 | 33 | 427905 | TGTGAGTAAGTTGCGCGATATTCTGCTGGATTC | 1 | False | negative3278067 | 1.1 | False | out | file.fasta | heidelberg | |
|
726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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216 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+ |
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217 | negative3299717-2.2.3.1.4 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 270552 | 270584 | 2E-11 | 62.1 | 33 | 427905 | ATGCCGGACAGCAGGCGAAACTCGAACCGGATA | 1 | False | negative3299717 | 2.2.3.1.4 | False | out | file.fasta | heidelberg | |
|
726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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218 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+ |
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219 | negative3373069-2.2.2.2.1.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 344011 | 344043 | 2E-11 | 62.1 | 33 | 427905 | CTCTCCAGAAGATGAAGCCCGTGATGCGGCGCA | 1 | False | negative3373069 | 2.2.2.2.1.1 | False | out | file.fasta | heidelberg | |
|
726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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220 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+ |
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221 |
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222 Next 196 lines omitted. |
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223 |
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224 |
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225 |
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226 Analysis of a single FASTQ readset |
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227 ---------------------------------- |
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228 |
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229 Contents of ``results.tab``: |
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230 |
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231 +--------+------------+-------------+------------------------------------------------+------------------------------------------+-------------------------+-----------------------+----------------------+----------------------------+---------------------------+---------------------------------+--------------------------+--------------------------------+------------------------------------------+ |
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232 | sample | scheme | subtype | all_subtypes | tiles_matching_subtype | are_subtypes_consistent | inconsistent_subtypes | n_tiles_matching_all | n_tiles_matching_all_total | n_tiles_matching_positive | n_tiles_matching_positive_total | n_tiles_matching_subtype | n_tiles_matching_subtype_total | file_path | |
|
726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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233 +--------+------------+-------------+------------------------------------------------+------------------------------------------+-------------------------+-----------------------+----------------------+----------------------------+---------------------------+---------------------------------+--------------------------+--------------------------------+------------------------------------------+ |
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planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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234 | 564 | heidelberg | 2.2.1.1.1.1 | 2; 2.2; 2.2.1; 2.2.1.1; 2.2.1.1.1; 2.2.1.1.1.1 | 1983064-2.2.1.1.1.1; 4211912-2.2.1.1.1.1 | True | | 202 | 202 | 20 | 20 | 2 | 2 | forward.fastqsanger; reverse.fastqsanger | |
|
726cd66ca2f9
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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235 +--------+------------+-------------+------------------------------------------------+------------------------------------------+-------------------------+-----------------------+----------------------+----------------------------+---------------------------+---------------------------------+--------------------------+--------------------------------+------------------------------------------+ |
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planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4ff4534765d620458c521bcac760cc8e01bc22f1
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236 |
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237 |
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238 Contents of ``match_results.tab``: |
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239 |
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240 +-----------------------------------+------+--------+------------------------------------------+------------------+-------------+-----------+-------------+-------------------+------------+ |
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241 | seq | freq | sample | file_path | tilename | is_pos_tile | subtype | refposition | is_kmer_freq_okay | scheme | |
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242 +-----------------------------------+------+--------+------------------------------------------+------------------+-------------+-----------+-------------+-------------------+------------+ |
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243 | ACGGTAAAAGAGGACTTGACTGGCGCGATTTGC | 68 | 564 | forward.fastqsanger; reverse.fastqsanger | 21097-2.2.1.1.1 | True | 2.2.1.1.1 | 21097 | True | heidelberg | |
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244 +-----------------------------------+------+--------+------------------------------------------+------------------+-------------+-----------+-------------+-------------------+------------+ |
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245 | AACCGGCGGTATTGGCTGCGGTAAAAGTACCGT | 77 | 564 | forward.fastqsanger; reverse.fastqsanger | 157792-2.2.1.1.1 | True | 2.2.1.1.1 | 157792 | True | heidelberg | |
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246 +-----------------------------------+------+--------+------------------------------------------+------------------+-------------+-----------+-------------+-------------------+------------+ |
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247 | CCGCTGCTTTCTGAAATCGCGCGTCGTTTCAAC | 67 | 564 | forward.fastqsanger; reverse.fastqsanger | 293728-2.2.1.1 | True | 2.2.1.1 | 293728 | True | heidelberg | |
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248 +-----------------------------------+------+--------+------------------------------------------+------------------+-------------+-----------+-------------+-------------------+------------+ |
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249 | GAATAACAGCAAAGTGATCATGATGCCGCTGGA | 91 | 564 | forward.fastqsanger; reverse.fastqsanger | 607438-2.2.1 | True | 2.2.1 | 607438 | True | heidelberg | |
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250 +-----------------------------------+------+--------+------------------------------------------+------------------+-------------+-----------+-------------+-------------------+------------+ |
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251 | CAGTTTTACATCCTGCGAAATGCGCAGCGTCAA | 87 | 564 | forward.fastqsanger; reverse.fastqsanger | 691203-2.2.1.1 | True | 2.2.1.1 | 691203 | True | heidelberg | |
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252 +-----------------------------------+------+--------+------------------------------------------+------------------+-------------+-----------+-------------+-------------------+------------+ |
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253 | CAGGAGAAAGGATGCCAGGGTCAACACGTAAAC | 33 | 564 | forward.fastqsanger; reverse.fastqsanger | 944885-2.2.1.1.1 | True | 2.2.1.1.1 | 944885 | True | heidelberg | |
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254 +-----------------------------------+------+--------+------------------------------------------+------------------+-------------+-----------+-------------+-------------------+------------+ |
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255 |
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256 Next 200 lines omitted. |
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257 |
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258 ]]></help> |
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259 <citations> |
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260 <citation type="bibtex">@ARTICLE{a1, |
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261 title = {A robust genotyping scheme for *Salmonella enterica* serovar Heidelberg clones circulating in North America.}, |
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262 author = {Geneviève Labbé, James Robertson, Peter Kruczkiewicz, Chad R. Laing, Kim Ziebell, Aleisha R. Reimer, Lorelee Tschetter, Gary Van Domselaar, Sadjia Bekal, Kimberley A. MacDonald, Linda Hoang, Linda Chui, Danielle Daignault, Durda Slavic, Frank Pollari, E. Jane Parmley, Philip Mabon, Elissa Giang, Lok Kan Lee, Jonathan Moffat, Marisa Rankin, Joanne MacKinnon, Roger Johnson, John H.E. Nash.}, |
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263 url = {https://github.com/phac-nml/bio_hansel} |
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264 } |
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265 }</citation> |
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266 </citations> |
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267 </tool> |
