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1 <tool id="affr2vcf" name="Convert Affymetrix" version="1.9">
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2 <description>genotype calls and intensities to VCF</description>
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3 <requirements>
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4 <requirement type="package" version="1.9">bcftools-gtc2vcf-plugin</requirement>
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5 </requirements>
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6 <command detect_errors="exit_code"><![CDATA[
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7 #set text_outputs_dir = 'text_outputs'
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8 mkdir $text_outputs_dir &&
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9 bcftools +affy2vcf.so
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10 --fasta_ref '$reference_genome.fasta_ref'
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11 --annot '$annot'
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12 --snp-posteriors '$text_outputs_dir/snp_posteriors.txt'
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13 --summary '$text_outputs_dir/summary.txt'
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14 --report '$text_outputs_dir/report.txt'
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15 --calls '$text_outputs_dir/calls.txt'
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16 --confidences '$text_outputs_dir/confidences.txt'
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17 #if str($output_gender_estimate_cond.output_gender_estimate) == "yes"
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18 --sex '$text_outputs_dir/gender_estimate.txt'
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19 #end if
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20 #if str($append_version_cond.append_version) == "no":
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21 --no-version
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22 #end if
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23 --output '$output'
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24 --output_type $output_type
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25 --threads \${GALAXY_SLOTS:-4}
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26 ]]></command>
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27 <inputs>
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28 <param name="annot" type="data" format="csv" label="Probeset annotation file" />
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29 <conditional name="reference_genome">
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30 <param name="source" type="select" label="Will you select a reference reference genome from your history or use a locally cached genome index?">
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31 <option value="history" selected="true">Use a reference genome from my history</option>
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32 <option value="cached">Use a locally cached genome index</option>
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33 </param>
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34 <when value="history">
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35 <param name="fasta_ref" type="data" format="fasta" label="Select reference genome" />
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36 </when>
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37 <when value="cached">
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38 <param name="fasta_ref" type="select" format="fasta" label="Fasta reference sequence">
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39 <options from_data_table="all_fasta">
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40 <column name="name" index="1"/>
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41 <column name="value" index="2"/>
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42 <column name="path" index="2"/>
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43 <filter type="sort_by" column="1"/>
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44 <validator type="no_options" message="No cached Fasta genome references are available." />
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45 </options>
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46 </param>
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47 </when>
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48 </conditional>
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49 <conditional name="output_gender_estimate_cond">
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50 <param name="output_gender_estimate" type="select" force_select="true" label="Output apt-probeset-genotype gender estimate?">
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51 <option value="no" selected="true">No</option>
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52 <option value="yes">Yes</option>
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53 </param>
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54 <when value="no" />
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55 <when value="yes"/>
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56 </conditional>
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57 <conditional name="append_version_cond">
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58 <param name="append_version" type="select" force_select="true" label="Append version and command line to the header?">
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59 <option value="no" selected="true">No</option>
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60 <option value="yes">Yes</option>
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61 </param>
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62 <when value="no" />
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63 <when value="yes"/>
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64 </conditional>
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65 </inputs>
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66 <outputs>
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67 <collection name="txt_outputs" type="list" label="${tool.name} (reports) on ${on_string}">
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68 <discover_datasets pattern="__name__" directory="output" format="txt" />
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69 </collection>
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70 <data name="output" format="vcf" />
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71 </outputs>
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72 <tests>
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73 <test>
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74 </test>
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75 </tests>
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76 <help>
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77 This tool converts Affymetrix genotype calls and intensity files to VCF format.
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78
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79 -----
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80
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81 **Required options**
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82
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83 **Other options**
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84 </help>
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85 <citations>
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86 <citation type="bibtex">
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87 @misc{None,
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88 journal = {None},
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89 author = {Genovese, Giulio},
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90 title = {None},
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91 year = {None},
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92 url = {https://github.com/freeseek/gtc2vcf},}
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93 </citation>
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94 </citations>
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95 </tool>
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