Mercurial > repos > galaxyp > custom_pro_db

annotate customProDB.xml @ 7:b83a4002aab1 draft

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author galaxyp
date Fri, 06 May 2016 15:56:07 -0400
parents 61e45c111ef7
children 328a1e3b5261
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1 <tool id="custom_pro_db" name="CustomProDB" version="1.10.0">
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2 <description>Generate protein FASTAs from exosome or transcriptome data</description>
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3 <stdio>
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4 <exit_code range="1:" level="fatal" description="Job Failed" />
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5 </stdio>
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6 <command interpreter="Rscript --vanilla">customProDB.R
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7
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8 --bam="$genome_annotation.bamInput"
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9 --bai="${genome_annotation.bamInput.metadata.bam_index}"
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10 --vcf="$genome_annotation.vcfInput"
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11 --rpkmCutoff=$rpkmCutoff
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12 --outputFile="${output_rpkm}"
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14 #if str($genome_annotation.source) == "history":
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15 --exon_anno="$genome_annotation.exonAnno"
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16 --proteinseq="$genome_annotation.proteinSeq"
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17 --procodingseq="$genome_annotation.proCodingSeq"
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18 --ids="$genome_annotation.ids"
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19 #else:
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20 #set index_path = $genome_annotation.builtin.fields.path
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21 --exon_anno="$index_path/exon_anno.RData"
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22 --proteinseq="$index_path/proseq.RData"
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23 --procodingseq="$index_path/procodingseq.RData"
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24 --ids="$index_path/ids.RData"
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25 #end if
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27 2&gt;&amp;1</command>
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28 <inputs>
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29 <conditional name="genome_annotation">
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30 <param name="source" type="select" label="Will you select a genome annotation from your history or use a built-in annotation?" help="See `Annotations` section of help below">
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31 <option value="builtin">Use a built-in genome annotation</option>
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32 <option value="history">Use annotation from your history</option>
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33 </param>
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34 <when value="builtin">
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35 <param name="builtin" type="select" label="Select genome annotation" help="If your genome of interest is not listed, contact the Galaxy team">
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36 <options from_data_table="customProDB">
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37 <filter type="sort_by" column="2"/>
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38 <validator type="no_options" message="No annotations are available for the selected input dataset"/>
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39 </options>
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40 </param>
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41 <param name="bamInput" type="data" format="bam" label="BAM file">
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42 <validator type="unspecified_build" />
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43 <validator message="Sequences are not currently available for the specified build." metadata_column="1" metadata_name="dbkey" table_name="customProDB" type="dataset_metadata_in_data_table" />
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44 </param>
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45 <!--<param name="baiInput" type="data" format="bam_index" label="BAM Index (BAI) file">
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46 <validator type="unspecified_build" />
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47 <validator message="Sequences are not currently available for the specified build." metadata_column="1" metadata_name="dbkey" table_name="customProDB" type="dataset_metadata_in_data_table" />
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48 </param>-->
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49 <param name="vcfInput" type="data" format="vcf" label="VCF file">
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50 <validator type="unspecified_build" />
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51 <validator message="Sequences are not currently available for the specified build." metadata_column="1" metadata_name="dbkey" table_name="customProDB" type="dataset_metadata_in_data_table" />
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52 </param>
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53 </when>
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54 <when value="history">
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55 <param name="exonAnno" type="data" format="RData" metadata_name="dbkey" help="A dataframe of exon annotations in an RData file" label="Exon Annotations" />
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56 <param name="proteinSeq" type="data" format="RData" metadata_name="dbkey" help="A dataframe containing protein ids and protein sequences in an RData file" label="Protein Sequences" />
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57 <param name="proCodingSeq" type="data" format="RData" metadata_name="dbkey" help="A dataframe cotaining coding sequences for each protein in an RData file" label="Protein Coding Sequences" />
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58 <param name="ids" type="data" format="RData" metadata_name="dbkey" help="A dataframe cotaining IDs for each protein in an RData file" label="Protein IDs" />
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59 <param name="bamInput" type="data" format="bam" label="BAM file">
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60 <validator check="bam_index" message="Metadata missing, click the pencil icon in the history item and use the auto-detect feature to correct this issue." type="metadata" />
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61 </param>
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62 <param name="baiInput" type="data" format="bam_index" label="BAM Index file">
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63 <validator check="dbkey" message="Metadata missing, click the pencil icon in the history item and use the auto-detect feature to correct this issue." type="metadata" />
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64 </param>
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65 <param name="vcfInput" type="data" format="vcf" label="VCF file" />
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66 </when>
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67 </conditional>
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68 <param name="rpkmCutoff" type="float" value="1" min="0" label="Transcript Expression Cutoff (RPKM)" help="If non-zero, if a transcript does not meet this expression cutoff (based on RPKM) then it will not be included in the output database." />
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69 </inputs>
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70 <outputs>
6
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71 <data format="fasta" name="output_rpkm" from_work_dir="output_rpkm.fasta" label="${genome_annotation.bamInput.name.rsplit('.',1)[0]}_rpkm.fasta"/>
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72 <data format="fasta" name="output_snv" from_work_dir="output_snv.fasta" label="${genome_annotation.bamInput.name.rsplit('.',1)[0]}_snv.fasta"/>
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73 <data format="fasta" name="output_indel" from_work_dir="output_indel.fasta" label="${genome_annotation.bamInput.name.rsplit('.',1)[0]}_indel.fasta"/>
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74 </outputs>
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75 <tests>
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76 <test>
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77 <param name="bamInput" value="test1_sort.bam" dbkey="hg19" />
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78 <param name="vcfInput" value="test1.vcf" dbkey="hg19" />
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79 <param name="source" value="history" />
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80 <param name="exonAnno" value="exon_anno.RData" />
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81 <param name="proteinSeq" value="proseq.RData" />
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82 <param name="proCodingSeq" value="procodingseq.RData" />
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83 <param name="ids" value="ids.RData" />
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84 <output name="output_rpkm" file="test_rpkm.fasta" />
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85 <output name="output_snv" file="test_snv.fasta" />
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86 <output name="output_indel" file="test_indel.fasta" />
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87 </test>
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88 </tests>
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89 <help>
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90 **Description**
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91
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92 Generate protein FASTAs from exosome or transcriptome data (in the form of BAM files).
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93 </help>
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94 </tool>