Mercurial > repos > eschen42 > w4mcorcov

annotate w4mcorcov.xml @ 6:0b49916c5c52 draft

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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
author eschen42
date Wed, 05 Sep 2018 19:24:47 -0400
parents 1d046f648b47
children ca9938f2eb6a
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6
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1 <tool id="w4mcorcov" name="OPLS-DA_Contrasts" version="0.98.15">
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2 <description>OPLS-DA Contrasts of Univariate Results</description>
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3 <macros>
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4 <xml name="paramPairSigFeatOnly">
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5 <param name="pairSigFeatOnly" type="boolean" checked="true" truevalue="TRUE" falsevalue="FALSE"
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6 label="Retain only pairwise-significant features"
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7 help="When this option is set to 'Yes', analysis will be performed including only features that differ significantly for the pair of levels being contrasted; when set to 'No', any feature that varies significantly across all levels will be included (i.e., exclude any feature that is not significantly different across all levels). See examples below." />
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8 </xml>
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9 <xml name="cplots">
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10 <param name="cplot_y" label="C-plot Y-axis" type="select" help="Choose the Y-axis for C-plots.">
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11 <option value="correlation">Plot VIP versus correlation</option>
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12 <option value="covariance">Plot VIP versus covariance</option>
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13 </param>
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14 <param name="cplot_p" type="boolean" checked="true" truevalue="TRUE" falsevalue="FALSE"
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15 label="Produce predictor C-plot"
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16 help="When this option is set to 'Yes', correlation will be plotted against vip4 for predictor loadings." />
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17 <param name="cplot_o" type="boolean" checked="true" truevalue="TRUE" falsevalue="FALSE"
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18 label="Produce orthogonal C-plot"
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19 help="When this option is set to 'Yes', correlation will be plotted against vip4 for orthogonal loadings." />
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20 </xml>
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21 </macros>
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22 <requirements>
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23 <!--
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24 <requirement type="package" version="3.4.1">r-base</requirement>
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25 <requirement type="package" version="1.1_4">r-batch</requirement>
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26 <requirement type="package" version="1.2.14">bioconductor-ropls</requirement>
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27 -->
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28 <requirement type="package">r-base</requirement>
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29 <requirement type="package">r-batch</requirement>
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30 <requirement type="package" version="1.10.0">bioconductor-ropls</requirement>
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31 </requirements>
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32 <command detect_errors="aggressive"><![CDATA[
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33 Rscript '$__tool_directory__/w4mcorcov_wrapper.R'
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34 dataMatrix_in '$dataMatrix_in'
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35 sampleMetadata_in '$sampleMetadata_in'
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36 variableMetadata_in '$variableMetadata_in'
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37 facC '$facC'
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38 #if str( $signif_test.tesC ) == "none":
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39 tesC "none"
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40 pairSigFeatOnly "FALSE"
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41 #else:
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42 tesC '$signif_test.tesC'
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43 pairSigFeatOnly '$signif_test.pairSigFeatOnly'
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44 #end if
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45 levCSV '$levCSV'
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46 matchingC '$matchingC'
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47 labelFeatures '$labelFeatures'
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48 #if str( $xplots.expPlot ) == "none":
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49 cplot_p "FALSE"
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50 cplot_o "FALSE"
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51 cplot_y "correlation"
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52 #else if str( $xplots.expPlot ) == "cplot":
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53 cplot_p '$xplots.cplot_p'
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54 cplot_o '$xplots.cplot_o'
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55 cplot_y '$xplots.cplot_y'
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56 #end if
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57 contrast_detail '$contrast_detail'
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58 contrast_corcov '$contrast_corcov'
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59 contrast_salience '$contrast_salience'
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60 ]]></command>
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61
6
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62 <inputs>
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63 <param name="dataMatrix_in" format="tabular" label="Data matrix file" type="data"
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64 help="variables &#10006; samples" />
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65 <param name="sampleMetadata_in" format="tabular" label="Sample metadata file" type="data"
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66 help="sample metadata, one row per sample" />
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67 <param name="variableMetadata_in" format="tabular" label="Variable metadata file (ideally from Univariate)"
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68 type="data" help="variable metadata, one row per variable" />
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69 <param name="facC" type="text"
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70 label="Factor of interest"
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71 help="REQUIRED - The name of the column of sampleMetadata corresponding to the qualitative variable used to define the contrasts. Except when the 'Univariate Significance-test' is set to 'none', this also must be a portion of the column names in the variableMetadata file.">
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72 <sanitizer>
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73 <valid initial="string.letters">
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74 <add preset="string.digits"/>
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75 <add value="&#46;" /> <!-- dot, period -->
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76 <add value="&#95;" /> <!-- underscore -->
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77 <!-- R does not permit dashes in column names; neither does SQL -->
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78 </valid>
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79 </sanitizer>
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80 </param>
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81 <conditional name="signif_test">
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82 <param name="tesC" label="Univariate significance-test" type="select" help="Either 'none' or the name of the statistical test that was run by the 'Univariate' tool to produce the variableMetadata file; that name must also be a portion of the column names in that file.">
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83 <option value="none">none - Display all features from variableMetadata (rather than choosing a subset based on significance in univariate testing)</option>
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84 <option value="ttest">ttest - Student's t-test (parametric test, qualitative factor with exactly 2 levels)</option>
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85 <option value="anova">anova - Analysis of variance (parametric test, qualitative factor with more than 2 levels)</option>
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86 <option value="wilcoxon">wilcoxon - Wilcoxon rank test (nonparametric test, qualitative factor with exactly 2 levels)</option>
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87 <option value="kruskal">kruskal - Kruskal-Wallis rank test (nonparametric test, qualitative factor with more than 2 levels)</option>
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88 </param>
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89 <when value="none" />
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90 <when value="ttest">
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91 <expand macro="paramPairSigFeatOnly" />
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92 </when>
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93 <when value="anova">
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94 <expand macro="paramPairSigFeatOnly" />
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95 </when>
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96 <when value="wilcoxon">
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97 <expand macro="paramPairSigFeatOnly" />
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98 </when>
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99 <when value="kruskal">
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100 <expand macro="paramPairSigFeatOnly" />
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101 </when>
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102 </conditional>
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103 <param name="levCSV" type="text" value="*" label="Levels of interest"
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104 help="Comma-separated level-names (or comma-less regular expressions to match level-names) to consider in analysis; must match at least two levels; levels must be non-numeric; may include wild cards or regular expressions. Note that extra space characters will affect results - 'a,b' is correct, but 'a , b' is not and may fail or give different results.">
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105 <sanitizer>
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106 <valid initial="string.letters">
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107 <add preset="string.digits"/>
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108 <add value="&#36;" /> <!-- $ dollar, dollar-sign -->
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109 <add value="&#40;" /> <!-- ( left-paren -->
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110 <add value="&#41;" /> <!-- ) right-paren -->
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111 <add value="&#42;" /> <!-- * splat, asterisk -->
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112 <add value="&#43;" /> <!-- + plus -->
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113 <add value="&#44;" /> <!-- , comma -->
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114 <add value="&#45;" /> <!-- - dash, minus-sign -->
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115 <add value="&#46;" /> <!-- . dot, period -->
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116 <add value="&#58;" /> <!-- : colon -->
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117 <add value="&#59;" /> <!-- ; semi, semicolon -->
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118 <add value="&#63;" /> <!-- ? what, question mark -->
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119 <add value="&#91;" /> <!-- [ l-squib, left-squre-bracket -->
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120 <add value="&#92;" /> <!-- \ whack, backslash -->
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121 <add value="&#93;" /> <!-- ] r-squib, right-squre-bracket -->
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122 <add value="&#94;" /> <!-- ^ hat, caret -->
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123 <add value="&#95;" /> <!-- underscore -->
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124 <add value="&#123;" /> <!-- { l-cube, left-curly-bracket -->
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125 <add value="&#124;" /> <!-- | pipe -->
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126 <add value="&#125;" /> <!-- } r-cube, right-curly-bracket -->
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127 <!-- IMPORTANT - Note that single and double quotes are not part of this list; they have the potential to make the 'command' section insecure or broken. -->
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128 </valid>
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129 </sanitizer>
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130 </param>
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131 <param name="matchingC" label="Level-name matching" type="select" help="How to specify level-names generically. (See help below for details on using wild cards or regular expressions.)">
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132 <option value="none">do no generic matching</option>
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133 <option value="wildcard" selected="true">use wild-cards for matching level-names (default)</option>
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134 <option value="regex">use regular expressions for matching level-names</option>
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135 </param>
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136 <param name="labelFeatures" type="text" value="3"
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137 label="How many features having extreme loadings should be labelled on cov-vs.-cor plot"
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138 help="Specify the number of features at each of the loading-extremes that should be labelled (with the name of the feature) on the covariance-vs.-correlation plot; specify 'ALL' to label all features or '0' to label no features; this choice has no effect on the OPLS-DA loadings plot."/>
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139 <conditional name="xplots">
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140 <param name="expPlot" label="Extra plots to include" type="select" help="Choosing 'none' hides further choices.">
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141 <option value="none">Do not include additonal extra plots.</option>
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142 <option value="cplot">Include C-plots (predictor-loading vs. 'vip4p' and orthogonal-loading versus 'vip4o')</option>
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143 </param>
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144 <when value="none" />
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145 <when value="cplot">
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146 <expand macro="cplots" />
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147 </when>
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148 </conditional>
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149 </inputs>
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150 <outputs>
0
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151 <!--
3
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152 pdf1: summaries of each contrasts, clearly labelled by level=pair name
0
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153 * first PCA score-plot
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154 * then PLS score-plot
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155 * then PLS S-PLOT; color in red features with VIP > 1; color in grey any non-pairwise-significant features, if these are included
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156 -->
6
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157 <data name="contrast_detail" format="pdf" label="${tool.name}_${variableMetadata_in.name}_detail" />
0
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158 <!--
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159 tsv1: cor and cov table with columns:
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160 * feature-ID
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161 * factor-level 1
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162 * factor-level 2, lexically greater than level 1
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163 * Wiklund_2008 correlation
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164 * Wiklund_2008 covariance
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165 * Galindo_Prieto_2014 VIP for predictive components, VIP[4,p]
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166 * Galindo_Prieto_2014 VIP for orthogonal components, VIP[4,o]
6
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167 * When filtering on significance of univariate tests,significance of test of null hypothesis that there is no difference between the two classes, i.e, the pair-wise test.
1
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168 -->
6
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169 <data name="contrast_corcov" format="tabular" label="${tool.name}_${variableMetadata_in.name}_corcov" />
1
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170 <!--
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171 tsv2: salience table with columns (experimental feature):
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172 * feature-ID
0
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173 * Salient level, i.e., for the feature, the class-level having the greatest median intensity
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174 * Salient robust coefficient of variation, i.e., for the feature, the mean absolute deviation of the intensity for the salient level divided by the median intensity for the salient level
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175 * Salience, i.e., for the feature, the median of the class-level having the greatest intensity divided by the mean of the medians for all class-levels.
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176 -->
6
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177 <data name="contrast_salience" format="tabular" label="${tool.name}_${variableMetadata_in.name}_salience" />
0
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178 </outputs>
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179 <tests>
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180 <!-- test #1 -->
0
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181 <test>
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182 <param name="dataMatrix_in" value="input_dataMatrix.tsv"/>
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183 <param name="sampleMetadata_in" value="input_sampleMetadata.tsv"/>
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184 <param name="variableMetadata_in" value="input_variableMetadata.tsv"/>
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185 <param name="tesC" value="kruskal"/>
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186 <param name="facC" value="k10"/>
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187 <param name="pairSigFeatOnly" value="FALSE"/>
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188 <param name="labelFeatures" value="3"/>
0
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189 <param name="levCSV" value="k[12],k[3-4]"/>
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190 <param name="matchingC" value="regex"/>
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191 <output name="contrast_corcov">
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192 <assert_contents>
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193 <!-- column-labels line -->
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194 <has_text text="featureID" />
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195 <has_text text="factorLevel1" />
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196 <has_text text="factorLevel2" />
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197 <has_text text="correlation" />
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198 <has_text text="covariance" />
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199 <has_text text="vip4p" />
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200 <has_text text="vip4o" />
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201 <has_text text="level1Level2Sig" />
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202 <!-- first matched line -->
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203 <has_text text="M349.2383T700" />
6
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204 <has_text text="-0.3704185" />
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205 <has_text text="-36.6668927" />
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206 <has_text text="0.4914638" />
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207 <has_text text="0.01302117" />
0
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208 <!-- second matched line -->
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209 <has_text text="M207.9308T206" />
6
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210 <has_text text="0.3235022" />
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211 <has_text text="5.97529097" />
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212 <has_text text="0.207196379" />
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213 <has_text text="0.04438632" />
1
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214 </assert_contents>
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215 </output>
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216 <output name="contrast_salience">
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217 <assert_contents>
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218 <!-- column-labels line -->
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219 <has_text text="featureID" />
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220 <has_text text="salientLevel" />
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221 <has_text text="salientRCV" />
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222 <has_text text="salience" />
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223 <!-- first matched line -->
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224 <has_text text="M349.2383T700" />
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225 <has_text text="0.659554" />
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226 <has_text text="8.81866595" />
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227 <!-- second matched line -->
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228 <has_text text="M207.9308T206" />
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229 <has_text text="0.0578578" />
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230 <has_text text="2.27527985" />
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231 <!-- third matched line -->
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232 <has_text text="M211.0607T263" />
0
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233 <has_text text="9999" />
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234 <has_text text="12.87766096" />
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235 </assert_contents>
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236 </output>
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237 </test>
6
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238 <!-- test #2 -->
0
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239 <test>
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240 <param name="dataMatrix_in" value="input_dataMatrix.tsv"/>
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241 <param name="sampleMetadata_in" value="input_sampleMetadata.tsv"/>
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242 <param name="variableMetadata_in" value="input_variableMetadata.tsv"/>
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243 <param name="tesC" value="kruskal"/>
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244 <param name="facC" value="k10"/>
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245 <param name="pairSigFeatOnly" value="TRUE"/>
2
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246 <param name="labelFeatures" value="3"/>
0
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247 <param name="levCSV" value="k[12],k[3-4]"/>
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248 <param name="matchingC" value="regex"/>
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249 <output name="contrast_corcov">
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250 <assert_contents>
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251 <!-- column-labels line -->
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252 <has_text text="featureID" />
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253 <has_text text="factorLevel1" />
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254 <has_text text="factorLevel2" />
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255 <has_text text="correlation" />
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256 <has_text text="covariance" />
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257 <has_text text="vip4p" />
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258 <has_text text="vip4o" />
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259 <has_text text="level1Level2Sig" />
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260 <!-- first matched line -->
6
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261 <has_text text="M200.005T296" />
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262 <has_text text="-0.24533821" />
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263 <has_text text="-3.3573953" />
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264 <has_text text="0.1157346" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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265 <has_text text="0.0647860" />
1
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266 </assert_contents>
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267 </output>
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268 <output name="contrast_salience">
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269 <assert_contents>
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270 <!-- column-labels line -->
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271 <has_text text="featureID" />
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272 <has_text text="salientLevel" />
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273 <has_text text="salientRCV" />
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274 <has_text text="salience" />
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275 <!-- first matched line -->
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276 <has_text text="M349.2383T700" />
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277 <has_text text="0.659554" />
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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278 <has_text text="8.81866595" />
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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279 <!-- second matched line -->
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280 <has_text text="M207.9308T206" />
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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281 <has_text text="0.0578578" />
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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282 <has_text text="2.27527985" />
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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283 <!-- third matched line -->
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284 <has_text text="M211.0607T263" />
0
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285 <has_text text="9999" />
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286 <has_text text="12.87766096" />
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287 </assert_contents>
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288 </output>
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289 </test>
6
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290 <!-- test #3 -->
0
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291 <test>
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292 <param name="dataMatrix_in" value="input_dataMatrix.tsv"/>
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293 <param name="sampleMetadata_in" value="input_sampleMetadata.tsv"/>
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294 <param name="variableMetadata_in" value="input_variableMetadata.tsv"/>
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295 <param name="tesC" value="none"/>
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296 <param name="facC" value="k10"/>
2
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297 <param name="labelFeatures" value="3"/>
0
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298 <param name="levCSV" value="k[12],k[3-4]"/>
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299 <param name="matchingC" value="regex"/>
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300 <output name="contrast_corcov">
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301 <assert_contents>
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302 <!-- column-labels line -->
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303 <has_text text="featureID" />
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304 <has_text text="factorLevel1" />
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305 <has_text text="factorLevel2" />
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306 <has_text text="correlation" />
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307 <has_text text="covariance" />
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308 <has_text text="vip4p" />
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309 <has_text text="vip4o" />
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310 <!-- first matched line -->
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311 <has_text text="M349.2383T700" />
6
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312 <has_text text="-0.37867079" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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diff changeset
313 <has_text text="-37.71066" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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314 <has_text text="0.5246766" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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diff changeset
315 <has_text text="0.0103341" />
0
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316 <!-- second matched line -->
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317 <has_text text="M207.9308T206" />
6
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diff changeset
318 <has_text text="0.31570433" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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diff changeset
319 <has_text text="5.86655640" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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diff changeset
320 <has_text text="0.2111623" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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321 <has_text text="0.0488654" />
1
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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322 </assert_contents>
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323 </output>
6
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324 <!-- test #4 -->
1
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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325 <output name="contrast_salience">
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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326 <assert_contents>
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327 <!-- column-labels line -->
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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328 <has_text text="featureID" />
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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diff changeset
329 <has_text text="salientLevel" />
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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330 <has_text text="salientRCV" />
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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diff changeset
331 <has_text text="salience" />
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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diff changeset
332 <!-- first matched line -->
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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diff changeset
333 <has_text text="M349.2383T700" />
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334 <has_text text="0.659554" />
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335 <has_text text="8.81866595" />
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336 <!-- second matched line -->
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337 <has_text text="M207.9308T206" />
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338 <has_text text="0.0578578" />
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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339 <has_text text="2.27527985" />
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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340 <!-- third matched line -->
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341 <has_text text="M211.0607T263" />
0
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342 <has_text text="9999" />
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343 <has_text text="12.87766096" />
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344 </assert_contents>
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345 </output>
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346 </test>
6
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347 <!-- test #4 -->
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348 <test>
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349 <param name="dataMatrix_in" value="issue1_input_dataMatrix.tsv"/>
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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350 <param name="sampleMetadata_in" value="issue1_input_sampleMetadata.tsv"/>
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351 <param name="variableMetadata_in" value="issue1_input_variableMetadata.tsv"/>
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352 <param name="tesC" value="none"/>
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353 <param name="facC" value="tissue_flowering"/>
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354 <param name="labelFeatures" value="3"/>
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355 <param name="levCSV" value="*"/>
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356 <param name="matchingC" value="wildcard"/>
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357 <output name="contrast_corcov">
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358 <assert_contents>
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359 <!-- column-labels line -->
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360 <has_text text="featureID" />
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361 <has_text text="factorLevel1" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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362 <has_text text="factorLevel2" />
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363 <has_text text="correlation" />
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364 <has_text text="covariance" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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365 <has_text text="vip4p" />
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366 <has_text text="vip4o" />
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367 <!-- first matched line -->
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368 <has_text text="NM516T251" />
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369 <has_text text="flower_yes" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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370 <has_text text="other" />
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371 <has_text text="0.03402807" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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372 <has_text text="0.03526926" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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373 <has_text text="0.43664386" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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374 <has_text text="0.587701897" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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375 <has_text text="0.026082688" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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376 <has_text text="0.0437742145" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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377 <has_text text="516.0845" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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378 <has_text text="250.8762" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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379 </assert_contents>
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380 </output>
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381 <output name="contrast_salience">
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382 <assert_contents>
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383 <!-- column-labels line -->
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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384 <has_text text="featureID" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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385 <has_text text="salientLevel" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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386 <has_text text="salientRCV" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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387 <has_text text="salience" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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388 <has_text text="mz" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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389 <has_text text="rt" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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390 <!-- first matched line -->
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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391 <has_text text="PM518T369" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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392 <has_text text="flower_yes" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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393 <has_text text="0.58655260" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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394 <has_text text="4.414469" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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395 <has_text text="518.1656" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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396 <has_text text="368.59817" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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397 </assert_contents>
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398 </output>
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399 </test>
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400 <!-- test #5 -->
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401 <test>
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402 <param name="dataMatrix_in" value="input_dataMatrix.tsv"/>
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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403 <param name="sampleMetadata_in" value="issue6_input_sampleMetadata.tsv"/>
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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404 <param name="variableMetadata_in" value="input_variableMetadata.tsv"/>
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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405 <param name="tesC" value="none"/>
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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406 <param name="facC" value="k._10"/>
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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407 <param name="labelFeatures" value="3"/>
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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408 <param name="levCSV" value="k1,k.2"/>
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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409 <param name="matchingC" value="none"/>
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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410 <output name="contrast_corcov">
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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411 <assert_contents>
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412 <!-- column-labels line -->
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413 <has_text text="featureID" />
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414 <has_text text="factorLevel1" />
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415 <has_text text="factorLevel2" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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416 <has_text text="correlation" />
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417 <has_text text="covariance" />
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418 <has_text text="vip4p" />
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419 <has_text text="vip4o" />
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420 <!-- first matched line -->
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421 <has_text text="M349.2383T700" />
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422 <has_text text="0.43361563" />
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423 <has_text text="37.76875778" />
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424 <has_text text="0.54672558" />
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425 <has_text text="0.3920409" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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426 <!-- second matched line -->
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427 <has_text text="M207.9308T206" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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428 <has_text text="-0.3365475" />
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429 <has_text text="-6.337903" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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430 <has_text text="0.270297" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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431 <has_text text="0.037661" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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432 </assert_contents>
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433 </output>
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434 </test>
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435 <!-- test #6 - issue 6 -->
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436 <test>
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437 <param name="dataMatrix_in" value="input_dataMatrix.tsv"/>
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438 <param name="sampleMetadata_in" value="issue6_input_sampleMetadata.tsv"/>
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439 <param name="variableMetadata_in" value="input_variableMetadata.tsv"/>
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440 <param name="tesC" value="none"/>
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441 <param name="facC" value="k._10"/>
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442 <param name="labelFeatures" value="3"/>
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443 <param name="levCSV" value="k_3,k-4"/>
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444 <param name="matchingC" value="none"/>
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445 <output name="contrast_corcov">
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446 <assert_contents>
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447 <!-- column-labels line -->
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448 <has_text text="featureID" />
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449 <has_text text="factorLevel1" />
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450 <has_text text="factorLevel2" />
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451 <has_text text="correlation" />
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452 <has_text text="covariance" />
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453 <has_text text="vip4p" />
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454 <has_text text="vip4o" />
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455 <!-- first matched line -->
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456 <has_text text="M349.2383T700" />
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457 <has_text text="-0.0435663" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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458 <has_text text="-1.9068114" />
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459 <has_text text="0.0304592" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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460 <has_text text="0.104748883" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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461 </assert_contents>
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462 </output>
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463 </test>
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464 <!-- test #6 - issue 8 -->
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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465 <test>
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466 <param name="dataMatrix_in" value="input_dataMatrix.tsv"/>
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467 <param name="sampleMetadata_in" value="issue8_input_sampleMetadata.tsv"/>
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468 <param name="variableMetadata_in" value="input_variableMetadata.tsv"/>
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469 <param name="tesC" value="none"/>
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470 <param name="facC" value="k._10"/>
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471 <param name="labelFeatures" value="3"/>
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472 <param name="levCSV" value="k_3,k-4"/>
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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473 <param name="matchingC" value="none"/>
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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474 <output name="contrast_corcov">
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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475 <assert_contents>
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476 <!-- column-labels line -->
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477 <has_text text="featureID" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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478 <has_text text="factorLevel1" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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479 <has_text text="factorLevel2" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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480 <has_text text="correlation" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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481 <has_text text="covariance" />
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482 <has_text text="vip4p" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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483 <has_text text="vip4o" />
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484 <!-- k1 rejected by levCSV, leaving only k_3 and k-4 -->
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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485 <not_has_text text="k1" />
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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486 <not_has_text text="other" />
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487 </assert_contents>
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488 </output>
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489 </test>
0
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490 </tests>
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491 <help><![CDATA[
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492
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493 **Run PLS-DA Contrasts of Univariate Results**
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494 ----------------------------------------------
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495
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496 **Author** - Arthur Eschenlauer (University of Minnesota, esch0041@umn.edu)
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497
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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498
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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499 Motivation
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500 ----------
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501
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502 OPLS-DA and the SIMCA S-PLOT (Wiklund *et al.*, 2008) may be employed to draw attention to metabolomic features that are potential biomarkers, i.e. features that are potentially useful to discriminate to which class a sample should be assigned (e.g. Sun *et al.*, 2016). Workflow4Metabolomics (W4M, Giacomoni *et al.*, 2014, Guitton *et al.*, 2017) provides a suite of tools for preprocessing and statistical analysis of LC-MS, GC-MS, and NMR metabolomics data; however, it does not (as of release 3.0) include a tool for making the equivalent of an S-PLOT.
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503
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504 The S-PLOT is computed from mean-centered, pareto-scaled data. This plot presents the correlation of the first score vector from an OPLS-DA model with the sample-variables used to produce that model versus the covariance of the scores with the sample-variables. For OPLS-DA, the first score vector represents the variation among the sample-variables that is related to the predictor (i.e., the contrasting factor).
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505
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506 The primary aims of this tool are:
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507
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508 - To compute and visualize multiple contrasts with OPLS-DA and the covariance vs. correlation plot.
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509 - To write the results to data files for use in further multivariate analysis or visualization.
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510
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511 Note: This tool only supports categorical factors with non-numeric level-names.
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512
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513 Description
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514 -----------
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515
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516 The purpose of the 'PLS-DA Contrasts' tool is to visualize GC-MS or LC-MS features that are possible biomarkers.
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517
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518 The W4M 'Univariate' tool (Th]]>&#233;<![CDATA[venot *et al.*, 2015) adds the results of family-wise corrected pairwise significance-tests as columns of the **variableMetadata** dataset.
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519 For instance, suppose that you ran Kruskal-Wallis testing for a column named 'cluster' in sampleMetadata that has values 'k1' and 'k2' and at least one other value.
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520
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521 - A column of variableMetadata would be labelled 'cluster_kruskal_sig' and would have values '1' and '0'; when the samples are grouped by 'cluster', '1' means that there is strong evidence against the hypothesis that there is no difference among the intensities for the feature across all sample-groups.
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522 - A column of variableMetadata would be labelled 'cluster_kruskal_k1.k2_sig' and would have values '1' and '0', where '1' means that there is significant evidence against the hypothesis that samples from sampleMetadata whose 'cluster' column contains 'k1' or 'k2' have the same intensity for that feature.
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523
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524 The 'PLS-DA Contrasts' tool produces graphics and data for OPLS-DA contrasts of feature-intensities between significantly different pairs of factor-levels. For each factor-level, the tool performs a contrast with all other factor-levels combined and then separately with each other factor-level.
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525
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526 **Along the left-to-right axis, the plots show the supervised projection of the variation explained by the predictor** (i.e., the factor specified when invoking the tool); **the top-to-bottom axis displays the variation that is orthogonal to the predictor level** (i.e., independent of it).
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527
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528 Although this tool can be used in a purely exploratory manner by supplying the variableMetadata file without the columns added by the W4M 'Univariate' tool, **the preferred workflow may be to use univariate testing to exclude features that are not significantly different and then to use OPLS-DA to visualize the differences identified in univariate testing** (Th]]>&#233;<![CDATA[venot *et al.*, 2015); an appropriate exception would be to visualize contrasts of a specific list of metabolites.
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529
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530 It must be stressed that there may be no *single* definitive computational approach to select features that are reliable biomarkers, especially from a small number of samples or experiments. A few possible choices are:
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531
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532 - picking features with maximum loadings along the projection parallel to the predictor (loadp),
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533 - examining extreme values on S-PLOTs
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534 - examining "variable importance in projection VIP for OPLS-DA" (Galindo-Prieto *et al.* 2014), and
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535 - examining a feature's "selectivity ratio" (Rajalahti *et al.*, 2009).
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536
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537 In this spirit, this tool reports the S-PLOT covariance and correlation (Wiklund *op. cit.*) and VIP metrics, and it introduces an informal "salience" metric to flag features that may merit attention without dimensional reduction; future versions may add selectivity ratio.
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538
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539 For a more systematic approach to biomarker identification, please consider the W4M 'biosigner' tool (Rinuardo *et al.* 2016), which applies three different identification metrics to the selection process.
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540
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541 Regardless of how any potential biomarker is identified, further validation analysis (e.g., independent confirmatory experiments) is needed before it is recommended for general application.
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542
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543
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544 W4M Workflow Position
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545 ---------------------
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546
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547 - Upstream tool: **Univariate** (category: Statistical Analysis) or (not generally recommended) any **Preprocessing** tool that produces or updates a 'variableMetadata' file.
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548 - Downstream tool categories: **Statistical Analysis**
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549
0
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550 Input files
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551 -----------
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552
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553 +----------------------+-----------+
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554 | File | Format |
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555 +======================+===========+
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556 | Data matrix | tabular |
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557 +----------------------+-----------+
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558 | Sample metadata | tabular |
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559 +----------------------+-----------+
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560 | Variable metadata | tabular |
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561 +----------------------+-----------+
0
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562
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563 Output files
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564 ------------
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565
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566 +-------------------------------------------+-----------+
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567 | File | Format |
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568 +===========================================+===========+
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569 | Contrast detail | pdf |
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570 +-------------------------------------------+-----------+
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571 | Contrast "corrlation and covariance" data | tabular |
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572 +-------------------------------------------+-----------+
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573 | Feature "salience" data | tabular |
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574 +-------------------------------------------+-----------+
0
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575
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576 Parameters
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577 ----------
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578
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579 [IN] Data matrix file
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580 | variable x sample **dataMatrix** (tabular separated values) file of the numeric data matrix, with '.' as decimal, and 'NA' for missing values; the table must not contain metadata apart from row and column names; the row and column names must be identical to the rownames of the sample and variable metadata, respectively (see below)
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581 |
0
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582
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583 [IN] Sample metadata file
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584 | sample x metadata **sampleMetadata** (tabular separated values) file of the numeric and/or character sample metadata, with '.' as decimal and 'NA' for missing values
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585 |
0
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586
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587 [IN] Variable metadata file
2
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588 | variable x metadata **variableMetadata** (tabular separated values) file of the numeric and/or character variable metadata, with '.' as decimal and 'NA' for missing values
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589 |
0
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590
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591 [IN] Test
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592 | Name of the **statistical test** - a component of column names in variable metadata table
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593 | May be one of 'none', 'ttest', 'gwilcoxon', 'anova', 'kruskal', 'pearson', 'spearman'
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594 |
0
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595
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596 [IN] Factor of interest
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597 | Name of the **column of sampleMetadata** corresponding to the qualitative or quantitative variable
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598 |
0
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599
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600 [IN] Retain only pairwise-significant features
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601 | *Note that when 'Test' is 'none', all features are included in the analysis and this parameter is not settable.*
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602 | When **true**, for each contrast of two levels, include only those features which pass the significance threshold for that contrast. Choosing true results in an OPLS-DA model that better reflects and visualizes the difference detected by univariate analysis, with somewhat increased reliability of prediction (as assessed by cross-validation).
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603 | When **false**, include all features that pass the significance threshold when testing for difference across all factor-levels. This choice produces a plot that displays more features but is not necessarily more informative.
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604 |
0
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605
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606 [IN] Levels of interest
2
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607 | Comma-separated **level-names** (or comma-less regular expressions to match level-names) to consider in analysis; must match at least two levels; may include wild cards or regular expressions.
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608 |
0
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609
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610 [IN] Level-name matching
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611 | Indicator of **how levels are to be specified generically** (if at all) - wild cards, regular expressions, or none (no generic matching).
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612 |
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613
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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614 [IN] Label how many extreme features
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615 | Specify the number of features at each of the loading-extremes that should be labelled (with the name of the feature) on the covariance-vs.-correlation plot; specify 'ALL' to label all features; this choice has no effect on the OPLS-DA loadings plot.
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616 |
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617
1
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618 [OUT] Contrast-detail output PDF
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619 | Several plots for each two-projection OPLS-DA analysis:
0
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620
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621 - (first row, left) **correlation-versus-covariance plot** of OPLS-DA results (a work-alike for the S-PLOT, computed using formula in Supplement to Wiklund, *op. cit.*); point-color becomes saturated as the "variable importance in projection to the predictive components" (VIP\ :subscript:`4,p` from Galindo-Prieto *et al.* 2014) ranges from 0.83 and 1.21 (Mehmood *et al.* 2012), for use to identify features for consideration as biomarkers.
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622 - (second row, left) **model-overview plot** for the two projections; grey bars are the correlation coefficient for the fitted data; black bars indicate performance in cross-validation tests (Th]]>&#233;<![CDATA[venot, 2017)
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623 - (first row, right) OPLS-DA **scores-plot** for the two projections (Th]]>&#233;<![CDATA[venot *et al.*, 2015)
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624 - (second row, right) **correlation-versus-covariance plot** of OPLS-DA results **orthogonal to the predictor** (see section "S-Plot of Orthogonal Component" in Wiklund, *op. cit.*, pp. 120-121; this characterizes features with the greatest variation independent of the predictor).
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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625 - (third row, left, when "**predictor C-plot**" is chosen under "Extra plots to include") plot of the covariance or correlation vs. the VIP for the projection *parallel to the predictor*, for use to identify features for consideration as biomarkers.
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626 - (third row, right, when "**orthogonal C-plot**" is chosen under "Extra plots to include") plotof the covariance or correlation vs. the VIP for the projection *orthogonal to the predictor*, for use to identify features varying considerably without regard to the predictor.
0
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627
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628 [OUT] Contrast Correlation-Covarinace data TABULAR
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629 | A tab-separated values file of metadata for each feature for each contrast in which it was included.
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630 | Thus, a given feature may appear many times, but *the combination of featureID, factorLevel1, and factorLevel2 will be unique.*
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631 | This file has the following columns:
0
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632
1
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633 - **featureID** - feature-identifier
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634 - **factorLevel1** - factor-level 1
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635 - **factorLevel2** - factor-level 2 (or "other" when contrasting factor-level 1 with all other levels)
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636 - **correlation** - correlation of the features projection explaining the difference between the features, < 0 when intensity for level 1 is greater (from formula in Supplement to Wiklund, *op. cit.*). Note that, for a given contrast, there is a linear relationship between 'loadp' and 'correlation'.
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637 - **covariance** - covariance of the features projection explaining the difference between the features, < 0 when intensity for level 1 is greater (from formula in *ibid.*)
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638 - **vip4p** - "variable importance in projection" to the predictive projection, VIP\ :subscript:`4,p` (Galindo-Prieto *op. cit.*)
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639 - **vip4o** - "variable importance in projection" to the orthogonal projection, VIP\ :subscript:`4,o` (*ibid.*)
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640 - **loadp** - variable loading for the predictive projection (Wiklund *op. cit.*)
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641 - **loado** - variable loading for the orthogonal projection (*ibid.*)
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642 - **level1Level2Sig** - (Only present when a test other than "none" is chosen) '1' when feature varies significantly across all classes (i.e., not pair-wise); '0' otherwise
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643
1
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644 [OUT] Feature "Salience" data TABULAR
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645 | Metrics for the "salient level" for each feature, i.e., the level at which the feature is more prominent than any other level. This is *not* at all related to the SIMCA OPLS-DA S-PLOT; rather, it is intended as a potential way to discover features for consideration as potential biomarkers without dimensionally reducting the data. This is a tab-separated values file having the following columns:
1
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646
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647 - **featureID** - feature identifier
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648 - **salientLevel** - salient level, i.e., for the feature, the class-level having the greatest median intensity
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649 - **salientRCV** - salient robust coefficient of variation, i.e., for the feature, the mean absolute deviation of the intensity for the salient level divided by the median intensity for the salient level
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650 - **salience** - salience, i.e., for the feature, the median of the class-level having the greatest intensity divided by the mean of the medians for all class-levels
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651
0
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652 Wild card patterns to match level-names
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653 ---------------------------------------
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654
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655 "wild card" patterns may be used to select level-names.
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656
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657 - use '``?``' to match a single character
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658 - use '``*``' to match zero or more characters
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659 - the entire pattern must match the level name
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660
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661 For example
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662
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663 - '``??.le*``' matches '``my.level``' but not '``my.own.level``'
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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664 - '``*.level``' matches '``my.level``' and '``my.own.level``'
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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diff changeset
665 - '``*.level``' matches neither '``my.level``' nor '``my.own.level``'
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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diff changeset
666
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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diff changeset
667 Regular expression patterns to match level-names
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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668 ------------------------------------------------
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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diff changeset
669
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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670 "regular expression" patterns may be used to select level-names.
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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diff changeset
671
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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672 POSIX 1003.2 standard regular expressions allow precise pattern-matching and are exhaustively defined at:
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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673 http://pubs.opengroup.org/onlinepubs/9699919799/basedefs/V1_chap09.html
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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diff changeset
674
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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675 However, only a few basic building blocks of regular expressions need to be mastered for most cases:
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diff changeset
676
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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677 - '``^``' matches the beginning of a level-name
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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678 - '``$``' matches the end of a level-name
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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679 - '``.``' outside of square brackets matches a single character
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680 - '``*``' matches character specified immediately before zero or more times
2
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681 - Square brackets specify a set of characters to be matched. Within square brackets:
0
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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682
2
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683 - '``^``' as the first character specifies that the list of characters are those that should **not** be matched.
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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684 - '``-``' is used to specify ranges of characters
0
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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685
2
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686 Caveat: The tool wrapper uses the comma ('``,``') to split a list of sample-level names, so **commas may not be used within regular expressions for this tool.**
0
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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diff changeset
687
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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688 First Example: Consider a field of level-names consisting of '``marq3,marq6,marq9,marq12,front3,front6,front9,front12``'
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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diff changeset
689
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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690 - The regular expression '``^front[0-9][0-9]*$``' will match the same sample-levels as '``front3,front6,front9,front12``'
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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691 - The regular expression '``^[a-z][a-z]3$``' will match the same sample-levels as '``front3,marq3``'
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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692 - The regular expression '``^[a-z][a-z]12$``' will match the same sample-levels as '``front12,marq12``'
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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693 - The regular expression '``^[a-z][a-z][0-9]$``' will match the same sample-levels as '``front3,front6,front9,marq3,marq6,marq9``'
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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diff changeset
694
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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695 Second Example: Consider these regular expression patterns as possible matches to a sample-level name '``AB0123``':
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diff changeset
696
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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697 - '``^[A-Z][A-Z][0-9][0-9]*$``' - MATCHES '``**^AB0123$**``'
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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698 - '``^[A-Z][A-Z]*[0-9][0-9]*$``' - MATCHES '``**^AB0123$**``'
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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699 - '``^[A-Z][0-9]*``' - MATCHES '``**^A** B0123$``' - first character is a letter, '``*``' can specify zero characters, and end of line did not need to be matched.
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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700 - '``^[A-Z][A-Z][0-9]``' - MATCHES '``**^AB0** 123$``' - first two characters are letters aind the third is a digit.
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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diff changeset
701 - '``^[A-Z][A-Z]*[0-9][0-9]$``' - NO MATCH - the name does not end with the pattern '``[A-Z][0-9][0-9]$``', i.e., it ends with four digits, not two.
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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parents:
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702 - '``^[A-Z][0-9]*$``' - NO MATCH - the pattern specifies that second character and all those that follow, if present, must be digits.
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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diff changeset
703
2
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diff changeset
704 Working examples
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
705 ----------------
0
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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diff changeset
706
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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707 **Input files**
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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diff changeset
708
6
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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diff changeset
709 +-----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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diff changeset
710 | Download from URL |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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diff changeset
711 +===============================================================================================================================================+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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diff changeset
712 | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/input_dataMatrix.tsv |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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diff changeset
713 +-----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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diff changeset
714 | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/input_sampleMetadata.tsv |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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diff changeset
715 +-----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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diff changeset
716 | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/input_variableMetadata.tsv |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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diff changeset
717 +-----------------------------------------------------------------------------------------------------------------------------------------------+
2
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
718
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
719 **Example 1:** Include in the analysis only features identified as pair-wise significant in the Univariate test.
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
720
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
721 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
722 | Input Parameter or Result | Value |
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
723 +============================================+========================================================================================================================================+
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
724 | Factor of interest | k10 |
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
725 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
726 | Univariate Significance-Test | kruskal |
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
727 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
728 | Retain only pairwise-significant features | Yes |
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
729 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
730 | Levels of interest | k[12],k[3-4] |
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
731 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
732 | Level-name matching | use regular expressions for matching level-names |
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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733 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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734 | Number of features having extreme loadings | ALL |
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
735 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
6
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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736 | Output primary table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_corcov.tsv |
2
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
737 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
6
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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738 | Output salience table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_salience.tsv |
2
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
739 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
6
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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740 | Output figures PDF | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_detail.pdf |
2
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
741 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
742
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
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diff changeset
743 **Example 2:** Include in the analysis only features identified as overall-significant in the Univariate test. Note that this even includes these features in contrasts where they were not determined to be pair-wise significant in the Univariate test. Thus, more features are included than in Example 1.
0
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
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diff changeset
744
6
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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diff changeset
745 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
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diff changeset
746 | Input Parameter or Result | Value |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
747 +============================================+============================================================================================================================================+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
748 | Factor of interest | k10 |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
749 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
750 | Univariate Significance-Test | kruskal |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
751 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
752 | Retain only pairwise-significant features | No |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
753 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
754 | Levels of interest | ``*`` |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
755 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
756 | Level-name matching | use wild cards for matching level-names |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
757 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
758 | Number of features having extreme loadings | 5 |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
759 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
760 | Output primary table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_corcov_all.tsv |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
761 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
762 | Output salience table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_salience_all.tsv |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
763 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
764 | Output figures PDF | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_detail_all.pdf |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
765 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
2
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
eschen42
parents: 1
diff changeset
766
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
eschen42
parents: 1
diff changeset
767 **Example 3:** Include all features in the analysis without regard to Univariate testing. Univariate testing is not even a pre-requisite to using the tool when 'none' is selected for the test. Thus, more features are included than in Example 2.
0
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
eschen42
parents:
diff changeset
768
6
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
769 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
770 | Input Parameter or Result | Value |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
771 +============================================+==============================================================================================================================================+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
772 | Factor of interest | k10 |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
773 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
774 | Univariate Significance-Test | none |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
775 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
776 | Levels of interest | k[12],k[3-4] |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
777 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
778 | Level-name matching | use regular expressions for matching level-names |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
779 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
780 | Number of features having extreme loadings | 0 |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
781 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
782 | Output primary table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_corcov_global.tsv |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
783 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
784 | Output salience table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_salience_global.tsv |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
785 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
786 | Output figures PDF | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_detail_global.pdf |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
787 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
2
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
eschen42
parents: 1
diff changeset
788
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
eschen42
parents: 1
diff changeset
789 **Example 4:** Analysis of a two-level factor (including all features). This suppresses the contrasts of "each factor vs. the aggregate of all the others".
a06344808ffc planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit ce5178ce51b80f242d24db555044e6afc530ac99
eschen42
parents: 1
diff changeset
790
6
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
791 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
792 | Input Parameter or Result | Value |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
793 +============================================+==============================================================================================================================================+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
794 | Factor of interest | lohi |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
795 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
796 | Univariate Significance-Test | none |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
797 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
798 | Levels of interest | low,high |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
799 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
800 | Level-name matching | use regular expressions for matching level-names |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
801 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
802 | Number of features having extreme loadings | 3 |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
803 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
804 | Output primary table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_corcov_lohi.tsv |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
805 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
806 | Output salience table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_salience_lohi.tsv |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
807 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
808 | Output figures PDF | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_detail_lohi.pdf |
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
parents: 5
diff changeset
809 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
0
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
eschen42
parents:
diff changeset
810
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
eschen42
parents:
diff changeset
811
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
eschen42
parents:
diff changeset
812 Trademarks
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
eschen42
parents:
diff changeset
813 ----------
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
eschen42
parents:
diff changeset
814
5
1d046f648b47 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 95c797ebec96af87d7eb17b491b40aed9ae30f24
eschen42
parents: 4
diff changeset
815 OPLS-DA, SIMCA, and S-PLOT are registered trademarks of the Umetrics company. http://umetrics.com/about-us/trademarks
0
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
eschen42
parents:
diff changeset
816
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
eschen42
parents:
diff changeset
817
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
eschen42
parents:
diff changeset
818 ]]></help>
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
eschen42
parents:
diff changeset
819 <citations>
6
0b49916c5c52 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 4428e3252d54c8a8e0e5d85e8eaaeb13e9b21de7
eschen42
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diff changeset
820 <!-- this tool -->
3
61935618f92c planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 7682e8e7ae2bfb926d94b414b9a1649389f33582
eschen42
parents: 2
diff changeset
821 <citation type="doi">10.5281/zenodo.1034784</citation>
0
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
eschen42
parents:
diff changeset
822 <!-- Galindo_Prieto_2014 Variable influence on projection (VIP) for OPLS -->
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
eschen42
parents:
diff changeset
823 <citation type="doi">10.1002/cem.2627</citation>
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
eschen42
parents:
diff changeset
824 <!-- Giacomoni_2014 W4M 2.5 -->
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
eschen42
parents:
diff changeset
825 <citation type="doi">10.1093/bioinformatics/btu813</citation>
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
eschen42
parents:
diff changeset
826 <!-- Guitton_2017 W4M 3.0 -->
50a07adddfbd planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 52e588e19fe93d83d221710bb75559c5700ba637
eschen42
parents:
diff changeset
827 <citation type="doi">10.1016/j.biocel.2017.07.002</citation>
1
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828 <!-- Mehmood_2012 PLS-based variable-selection -->
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829 <citation type="doi">10.1186/1748-7188-6-27</citation>
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830 <!-- Rajalahti_2009 Biomarker discovery using selectivity ratio -->
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831 <citation type="doi">10.1016/j.chemolab.2008.08.004</citation>
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832 <!-- Rinuardo 2016 -->
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833 <citation type="doi">10.3389/fmolb.2016.00026</citation>
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834 <!-- Sun_2016 Urinary Biomarkers for adolescent idiopathic scoliosis -->
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835 <citation type="doi">10.1038/srep22274</citation>
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836 <!-- Thevenot_2015 Urinary metabolome statistics -->
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837 <citation type="doi">10.1021/acs.jproteome.5b00354</citation>
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838 <!-- ropls package -->
e25fd8a13665 planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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839 <citation type="bibtex"><![CDATA[
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840 @incollection{Thevenot_ropls_2017,
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841 author = {Th{\'{e}}venot, Etienne A.},
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842 title = {ropls: PCA, PLS(-DA) and OPLS(-DA) for multivariate analysis and feature selection of omics data},
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843 publisher = {bioconductor.org},
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844 year = {2017},
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845 doi = {10.18129/B9.bioc.ropls},
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846 booktitle = {Bioconductor: Open source software for bioinformatics},
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847 address = {Roswell Park Cancer Institute},
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848 }
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849 ]]></citation>
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850 <!-- Wiklund_2008 OPLS PLS-DA and S-PLOT -->
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851 <citation type="doi">10.1021/ac0713510</citation>
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852 </citations>
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853 <!--
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854 vim:et:sw=4:ts=4
0
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855 -->
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856 </tool>