Mercurial > repos > drosofff > msp_sr_signature
annotate signature.xml @ 2:2b30861d95f4 draft
Uploaded
| author | mvdbeek |
|---|---|
| date | Sun, 29 Mar 2015 11:58:31 -0400 |
| parents | 9274c7b1e85c |
| children | 741cc1d16813 |
| rev | line source |
|---|---|
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1
9274c7b1e85c
Fixed issue: now the plot properly reflects a subset of analysed overlaps, i.e 5 to 15 nucleotides of overlap.
chris <drosofff@gmail.com>
parents:
0
diff
changeset
|
1 <tool id="signature" name="Small RNA Signatures" version="2.0.1"> |
| 2 | 2 <description /> |
| 3 <requirements> | |
| 4 <requirement type="package" version="0.12.7">bowtie</requirement> | |
| 5 <requirement type="package" version="0.1.18">samtools</requirement> | |
| 6 <requirement type="package" version="0.7.7">pysam</requirement> | |
| 7 <requirement type="package" version="2.14">biocbasics</requirement> | |
| 8 <requirement type="package" version="3.0.3">R</requirement> | |
| 9 <requirement type="package" version="1.9">numpy</requirement> | |
| 10 <requirement type="package" version="0.14">scipy</requirement> | |
| 11 </requirements> | |
| 12 <command interpreter="python"> | |
| 0 | 13 signature.py |
| 14 --input $refGenomeSource.input | |
| 15 --inputFormat $refGenomeSource.input.ext | |
| 16 --minquery $minquery | |
| 17 --maxquery $maxquery | |
| 18 --mintarget $mintarget | |
| 19 --maxtarget $maxtarget | |
| 20 --minscope $minscope | |
| 21 --maxscope $maxscope | |
| 22 --outputOverlapDataframe $output | |
| 23 #if $refGenomeSource.genomeSource == "history": | |
| 24 --referenceGenome $refGenomeSource.ownFile | |
| 25 #else: | |
| 26 #silent reference= filter( lambda x: str( x[0] ) == str( $input.dbkey ), $__app__.tool_data_tables[ 'bowtie_indexes' ].get_fields() )[0][-1] | |
| 27 --referenceGenome $reference | |
| 28 --extract_index | |
| 29 #end if | |
| 30 --graph $graph_type | |
| 31 --rcode $sigplotter | |
| 32 </command> | |
| 2 | 33 <inputs> |
| 34 <conditional name="refGenomeSource"> | |
| 35 <param help="Built-ins were indexed using default options" label="Will you select a reference genome from your history or use a built-in index?" name="genomeSource" type="select"> | |
| 36 <option value="indexed">Use a built-in index</option> | |
| 37 <option value="history">Use one from the history</option> | |
| 38 </param> | |
| 39 <when value="indexed"> | |
| 40 <param format="tabular,sam,bam" label="Compute signature from this bowtie standard output" name="input" type="data"> | |
| 41 <validator message="database not set for this bowtie output. Select the database(=genome used for matching) manually, or select a reference fasta from your history." metadata_column="0" metadata_name="dbkey" table_name="bowtie_indexes" type="dataset_metadata_in_data_table" /> | |
| 42 </param> | |
| 43 </when> | |
| 44 <when value="history"> | |
| 45 <param format="fasta" label="Select the fasta reference" name="ownFile" type="data" /> | |
| 46 <param format="tabular,sam,bam" label="Compute signature from this bowtie standard output" name="input" type="data" /> | |
| 47 </when> | |
| 48 </conditional> | |
| 49 <param help="'23' = 23 nucleotides" label="Min size of query small RNAs" name="minquery" size="3" type="integer" value="23" /> | |
| 50 <param help="'29' = 29 nucleotides" label="Max size of query small RNAs" name="maxquery" size="3" type="integer" value="29" /> | |
| 51 <param help="'23' = 23 nucleotides" label="Min size of target small RNAs" name="mintarget" size="3" type="integer" value="23" /> | |
| 52 <param help="'29' = 29 nucleotides" label="Max size of target small RNAs" name="maxtarget" size="3" type="integer" value="29" /> | |
| 53 <param help="'1' = 1 nucleotide overlap" label="Minimal relative overlap analyzed" name="minscope" size="3" type="integer" value="1" /> | |
| 54 <param help="'1' = 1 nucleotide overlap" label="Maximal relative overlap analyzed" name="maxscope" size="3" type="integer" value="26" /> | |
| 55 <param help="Signature can be computed globally or by item present in the alignment file" label="Graph type" name="graph_type" type="select"> | |
| 56 <option selected="True" value="global">Global</option> | |
| 57 <option value="lattice">Lattice</option> | |
| 58 </param> | |
| 59 </inputs> | |
| 60 <outputs> | |
| 61 <data format="tabular" label="signature data frame" name="output" /> | |
| 62 <data format="pdf" label="Overlap probabilities" name="output2" /> | |
| 63 </outputs> | |
| 64 <tests> | |
| 65 <test> | |
| 66 <param name="genomeSource" value="history" /> | |
| 67 <param ftype="fasta" name="ownFile" value="ensembl.fa" /> | |
| 68 <param ftype="bam" name="input" value="sr_bowtie.bam" /> | |
| 69 <param name="minquery" value="23" /> | |
| 70 <param name="maxquery" value="29" /> | |
| 71 <param name="mintarget" value="23" /> | |
| 72 <param name="maxtarget" value="29" /> | |
| 73 <param name="minscope" value="5" /> | |
| 74 <param name="maxscope" value="15" /> | |
| 75 <param name="graph_type" value="global" /> | |
| 76 <output file="signature.tab" ftype="tabular" name="output" /> | |
| 77 <output file="signature.pdf" ftype="pdf" name="output2" /> | |
| 78 </test> | |
| 79 </tests> | |
| 80 <help> | |
| 0 | 81 |
| 2 | 82 **What it does** |
| 83 | |
| 84 This tool computes the number of pairs by overlap classes (in nt) from a bowtie output file, the z-score calculated from these numbers of pairs, and the ping-pong signal as described in Brennecke et al (2009) Science. | |
| 85 The numerical options set the min and max size of both the query small rna class and the target small rna class. | |
| 86 Three type of signals are plotted in separate pdf files, the number of pairs founds, the z-score calculated from these numbers of pairs, and the ping-pong signal as described in Brennecke et al (2009) Science. | |
| 0 | 87 |
| 2 | 88 </help> |
| 89 <citations> | |
| 90 <citation type="doi">10.1007/978-1-4939-0931-5_12</citation> | |
| 91 </citations> | |
| 92 <configfiles> | |
| 93 <configfile name="sigplotter"> | |
| 0 | 94 graph_type = "${graph_type}" |
| 95 | |
| 96 globalgraph = function () { | |
| 97 ## Setup R error handling to go to stderr | |
| 98 options( show.error.messages=F, | |
| 99 error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) | |
| 100 signature = read.delim("${output}", header=TRUE) | |
|
1
9274c7b1e85c
Fixed issue: now the plot properly reflects a subset of analysed overlaps, i.e 5 to 15 nucleotides of overlap.
chris <drosofff@gmail.com>
parents:
0
diff
changeset
|
101 signaturez=data.frame(signature[,1], (signature[,2] -mean(signature[,2]))/sd(signature[,2])) |
|
9274c7b1e85c
Fixed issue: now the plot properly reflects a subset of analysed overlaps, i.e 5 to 15 nucleotides of overlap.
chris <drosofff@gmail.com>
parents:
0
diff
changeset
|
102 overlap_prob_z=data.frame(signature[,1], (signature[,3] -mean(signature[,3]))/sd(signature[,3])) |
| 0 | 103 YLIM=max(signature[,2]) |
| 104 | |
| 105 ## Open output2 PDF file | |
| 106 pdf( "${output2}" ) | |
| 107 par(mfrow=c(2,2),oma = c(0, 0, 3, 0)) | |
| 108 | |
| 109 plot(signature[,1:2], type = "h", main="Numbers of pairs", cex.main=1, xlab="overlap (nt)", ylim=c(0,YLIM), ylab="Numbers of pairs", col="darkslateblue", lwd=4) | |
| 110 | |
|
1
9274c7b1e85c
Fixed issue: now the plot properly reflects a subset of analysed overlaps, i.e 5 to 15 nucleotides of overlap.
chris <drosofff@gmail.com>
parents:
0
diff
changeset
|
111 plot(signaturez, type = "l", main="Number of pairs Z-scores", cex.main=1, xlab="overlap (nt)", ylab="z-score", pch=19, cex=0.2, col="darkslateblue", lwd=2) |
| 0 | 112 |
| 113 plot(signature[,1], signature[,3]*100, type = "l", main="Overlap probabilities", | |
| 114 cex.main=1, xlab="overlap (nt)", ylab="Probability [%]", ylim=c(0,50), | |
|
1
9274c7b1e85c
Fixed issue: now the plot properly reflects a subset of analysed overlaps, i.e 5 to 15 nucleotides of overlap.
chris <drosofff@gmail.com>
parents:
0
diff
changeset
|
115 pch=19, col="darkslateblue", lwd=2) |
| 0 | 116 |
|
1
9274c7b1e85c
Fixed issue: now the plot properly reflects a subset of analysed overlaps, i.e 5 to 15 nucleotides of overlap.
chris <drosofff@gmail.com>
parents:
0
diff
changeset
|
117 plot(overlap_prob_z, type = "l", main="Overlap Probability Z-scores", cex.main=1, xlab="overlap (nt)", ylab="z-score", pch=19, cex=0.2, col="darkslateblue", lwd=2) |
|
9274c7b1e85c
Fixed issue: now the plot properly reflects a subset of analysed overlaps, i.e 5 to 15 nucleotides of overlap.
chris <drosofff@gmail.com>
parents:
0
diff
changeset
|
118 |
| 0 | 119 mtext("Overlap Signatures of ${minquery}-${maxquery} against ${mintarget}-${maxtarget}nt small RNAs", outer = TRUE, cex=1) |
| 120 devname = dev.off() | |
| 121 ## Close the PDF file | |
| 122 } | |
| 123 | |
| 124 treillisgraph = function () { | |
| 125 ## Open output2 PDF file | |
| 126 pdf( "${output2}", paper="special", height=11.69, width=8.2677 ) | |
| 127 signature = read.delim("${output}", header=TRUE) | |
| 128 options( show.error.messages=F, | |
| 129 error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) | |
| 130 library(lattice) | |
|
1
9274c7b1e85c
Fixed issue: now the plot properly reflects a subset of analysed overlaps, i.e 5 to 15 nucleotides of overlap.
chris <drosofff@gmail.com>
parents:
0
diff
changeset
|
131 print (xyplot(signature[,3]*100~signature[,1]|signature[,4], type = "l", xlim=c(${minscope},${maxscope}), main="ping-pong Signature of ${minquery}-${maxquery} against ${mintarget}-${maxtarget}nt small RNAs", |
| 0 | 132 par.strip.text=list(cex=.5), strip=strip.custom(which.given=1, bg="lightblue"), scales=list(cex=0.5), |
| 133 cex.main=1, cex=.5, xlab="overlap (nt)", ylab="ping-pong signal [%]", | |
| 134 pch=19, col="darkslateblue", lwd =1.5, cex.lab=1.2, cex.axis=1.2, | |
| 135 layout=c(4,12), as.table=TRUE, newpage = T) ) | |
| 136 devnname = dev.off() | |
| 137 } | |
| 138 | |
| 139 if (graph_type=="global") { | |
|
1
9274c7b1e85c
Fixed issue: now the plot properly reflects a subset of analysed overlaps, i.e 5 to 15 nucleotides of overlap.
chris <drosofff@gmail.com>
parents:
0
diff
changeset
|
140 globalgraph() |
| 0 | 141 |
| 142 } | |
| 143 if(graph_type=="lattice") { | |
| 144 treillisgraph() | |
| 145 } | |
| 146 </configfile> | |
| 2 | 147 </configfiles> |
| 0 | 148 </tool> |