#!/usr/bin/python
# python parser module to analyse Features in sRbowtie alignments (guided by a GFF3 file)
# version 0.9
# Usage FeaturesParser.py  <1:index source> <2:extraction directive> <3:output> <4:GFF3 guide file> <5:6:7 filePath:FileExt:FileLabel> <.. ad  lib>

import sys
from smRtools import *
from collections import *

IndexSource = sys.argv[1]
ExtractionDirective = sys.argv[2]
if ExtractionDirective == "--do_not_extract_index":
  genomeRefFormat = "fastaSource"
elif  ExtractionDirective == "--extract_index":
  genomeRefFormat = "bowtieIndex"
Output = sys.argv[3]
GFF3_file = sys.argv[4]
Triplets = [sys.argv[5:][i:i+3] for i in xrange(0, len(sys.argv[5:]), 3)]
MasterListOfGenomes = {}
FeatureDict = defaultdict(dict)

for [filePath, FileExt, FileLabel] in Triplets:
  MasterListOfGenomes[FileLabel] = HandleSmRNAwindows (filePath, FileExt, IndexSource, genomeRefFormat) 
  FeatureDict[FileLabel] = MasterListOfGenomes[FileLabel].CountFeatures(GFF3=GFF3_file)

# add some code to pick up the GFF3 features in their order of appearence.
F = open(GFF3_file, "r")
featureList = []
for line in F:
  if line[0] == "#": continue
  feature = line.split()[2]
  if feature not in featureList:
    featureList.append(feature)
F.close()

header = ["#Feature"]
for [filePath, FileExt, FileLabel] in Triplets:
  header.append(FileLabel)

F = open (sys.argv[3], "w")
print >> F, "\t".join(header)
for feature in  featureList:
  line=[feature]
  for sample in header[1:]:
    count = str (FeatureDict[sample][feature])
# uncomment to get percentage in addition to counts
#    percent = float(FeatureDict[sample][feature]) / MasterListOfGenomes[sample].alignedReads
#    value = "%s | %0.2f" % (count, percent)
#    line.append(value)
    line.append(count)
  print >> F,  "\t".join(line )
line = ["Unfeatured"]
for sample in header[1:]:
  matched = 0
  for feature in FeatureDict[sample]:
    matched += FeatureDict[sample][feature]
  unmatched = MasterListOfGenomes[sample].alignedReads - matched
# uncomment to get percentage in addition to counts
#  percent = float (unmatched) / (matched + unmatched)
#  value = "%s | %0.2f" % (unmatched, percent)
#  line.append(value)
  line.append("%s" % unmatched)
print >> F,  "\t".join(line)
F.close()