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1 import re
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2 import argparse
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3
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4 parser = argparse.ArgumentParser()
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4
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5 parser.add_argument("--input", help="The '7_V-REGION-mutation-and-AA-change-table' and '10_V-REGION-mutation-hotspots' merged together, with an added 'best_match' annotation")
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6 parser.add_argument("--genes", help="The genes available in the 'best_match' column")
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0
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7 parser.add_argument("--output", help="Output file")
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8
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9 args = parser.parse_args()
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10
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4
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11 infile = args.input
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12 genes = str(args.genes).split(",")
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13 outfile = args.output
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14
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15 genedic = dict()
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0
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16
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17 mutationdic = dict()
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18 mutationMatcher = re.compile("^(.)(\d+).(.),?(.)?(\d+)?.?(.)?(.?.?.?.?.?)?")
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19 linecount = 0
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20
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4
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21 IDIndex = 0
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22 best_matchIndex = 0
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23 fr1Index = 0
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24 cdr1Index = 0
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25 fr2Index = 0
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26 cdr2Index = 0
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27 fr3Index = 0
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28 first=True
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26
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29 IDlist = []
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30 mutationList = []
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31
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4
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32 with open(infile, 'r') as i:
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33 for line in i:
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34 if first:
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35 linesplt = line.split("\t")
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36 IDIndex = linesplt.index("Sequence.ID")
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37 best_matchIndex = linesplt.index("best_match")
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38 fr1Index = linesplt.index("FR1.IMGT")
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39 cdr1Index = linesplt.index("CDR1.IMGT")
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40 fr2Index = linesplt.index("FR2.IMGT")
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41 cdr2Index = linesplt.index("CDR2.IMGT")
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42 fr3Index = linesplt.index("FR3.IMGT")
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43 first = False
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44 continue
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0
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45 linecount += 1
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46 linesplt = line.split("\t")
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4
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47 ID = linesplt[IDIndex]
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48 genedic[ID] = linesplt[best_matchIndex]
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49 mutationdic[ID + "_FR1"] = [mutationMatcher.match(x).groups() for x in linesplt[fr1Index].split("|") if x]
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50 mutationdic[ID + "_CDR1"] = [mutationMatcher.match(x).groups() for x in linesplt[cdr1Index].split("|") if x]
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51 mutationdic[ID + "_FR2"] = [mutationMatcher.match(x).groups() for x in linesplt[fr2Index].split("|") if x]
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52 mutationdic[ID + "_CDR2"] = [mutationMatcher.match(x).groups() for x in linesplt[cdr2Index].split("|") if x]
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0
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53 mutationdic[ID + "_FR2-CDR2"] = mutationdic[ID + "_FR2"] + mutationdic[ID + "_CDR2"]
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4
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54 mutationdic[ID + "_FR3"] = [mutationMatcher.match(x).groups() for x in linesplt[fr3Index].split("|") if x]
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26
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55
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56 mutationList += mutationdic[ID + "_FR1"] + mutationdic[ID + "_CDR1"] + mutationdic[ID + "_FR2"] + mutationdic[ID + "_CDR2"] + mutationdic[ID + "_FR3"]
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57
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58 IDlist += [ID]
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59
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60
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28
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61 AA_mutation = [0] * (int(max(mutationList, key=lambda i:int(i[4]) if i[4] else 0)[4]) + 1) #[4] is the position of the AA mutation, None if silent
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26
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62
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63 for mutation in mutationList:
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64 if mutation[4]: #if non silent mutation
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65 AA_mutation[int(mutation[4])] += 1
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66
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67 aa_mutations_file = outfile[:outfile.rindex("/")] + "/aa_mutations.txt"
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68 with open(aa_mutations_file, 'w') as o:
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69 o.write(",".join([str(x) for x in AA_mutation]) + "\n")
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0
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70
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71 if linecount == 0:
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72 print "No data, exiting"
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73 with open(outfile, 'w') as o:
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4
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74 o.write("RGYW (%)," + ("0,0,0\n" * len(genes)))
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75 o.write("WRCY (%)," + ("0,0,0\n" * len(genes)))
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76 o.write("WA (%)," + ("0,0,0\n" * len(genes)))
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77 o.write("TW (%)," + ("0,0,0\n" * len(genes)))
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0
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78 import sys
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79 sys.exit()
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80
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81 hotspotMatcher = re.compile("[actg]+,(\d+)-(\d+)\((.*)\)")
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4
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82 RGYWCount = {g: 0 for g in genes}
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83 WRCYCount = {g: 0 for g in genes}
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84 WACount = {g: 0 for g in genes}
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85 TWCount = {g: 0 for g in genes}
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0
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86
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4
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87 IDIndex = 0
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88 ataIndex = 0
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89 tatIndex = 0
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90 aggctatIndex = 0
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91 atagcctIndex = 0
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92 first = True
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93 with open(infile, 'r') as i:
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94 for line in i:
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95 if first:
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96 linesplt = line.split("\t")
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97 ataIndex = linesplt.index("X.a.t.a")
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98 tatIndex = linesplt.index("t.a.t.")
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99 aggctatIndex = linesplt.index("X.a.g.g.c.t..a.t.")
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100 atagcctIndex = linesplt.index("X.a.t..a.g.c.c.t.")
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101 first = False
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0
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102 continue
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4
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103 linesplt = line.split("\t")
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104 gene = linesplt[best_matchIndex]
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105 ID = linesplt[IDIndex]
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106 RGYW = [(int(x),int(y),z) for (x,y,z) in [hotspotMatcher.match(x).groups() for x in linesplt[aggctatIndex].split("|") if x]]
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107 WRCY = [(int(x),int(y),z) for (x,y,z) in [hotspotMatcher.match(x).groups() for x in linesplt[atagcctIndex].split("|") if x]]
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108 WA = [(int(x),int(y),z) for (x,y,z) in [hotspotMatcher.match(x).groups() for x in linesplt[ataIndex].split("|") if x]]
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109 TW = [(int(x),int(y),z) for (x,y,z) in [hotspotMatcher.match(x).groups() for x in linesplt[tatIndex].split("|") if x]]
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28
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110 RGYWCount[ID], WRCYCount[ID], WACount[ID], TWCount[ID] = 0,0,0,0
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111 for (x,y,z) in RGYW: #RGYWCount[ID] = sum([1 for (x,y,z) in RGYW if z and z != "CDR3" and any([(x <= int(where) <= y) for (frm, where, to, a,b,c,d) in mutationdic[ID + "_" + z]])])
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112 if not z or z == "CDR3":
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113 continue
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30
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114 mutations_in_motif = sum([(x <= int(where) <= y) for (frm, where, to, a,b,c,d) in mutationdic[ID + "_" + z]])
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115 in_other_motifs = sum([((x <= int(xother) <= y) and (x <= int(yother) <= y)) for (xother, yother, zother) in WRCY]) + sum([((x <= int(xother) <= y) and (x <= int(yother) <= y)) for (xother, yother, zother) in WA]) + sum([((x <= int(xother) <= y) and (x <= int(yother) <= y)) for (xother, yother, zother) in TW])
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116 if mutations_in_motif > 0:
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117 RGYWCount[ID] += 1.0 / in_other_motifs if in_other_motifs > 0 else 1
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118 if in_other_motifs > 1:
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119 print in_other_motifs, ID, "RGYW", x, y, ([(x,y,z) for (xother, yother, zother) in WRCY if ((x <= int(xother) <= y) and (x <= int(yother) <= y))] + [(x,y,z) for (xother, yother, zother) in WA if ((x <= int(xother) <= y) and (x <= int(yother) <= y))] + [(x,y,z) for (xother, yother, zother) in TW if ((x <= int(xother) <= y) and (x <= int(yother) <= y))])
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28
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120
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121 for (x,y,z) in WRCY: #WRCYCount[ID] = sum([1 for (x,y,z) in WRCY if z and z != "CDR3" and any([(x <= int(where) <= y) for (frm, where, to, a,b,c,d) in mutationdic[ID + "_" + z]])])
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122 if not z or z == "CDR3":
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123 continue
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30
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124 mutations_in_motif = sum([(x <= int(where) <= y) for (frm, where, to, a,b,c,d) in mutationdic[ID + "_" + z]])
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125 in_other_motifs = sum([((x <= int(xother) <= y) and (x <= int(yother) <= y)) for (xother, yother, zother) in RGYW]) + sum([((x <= int(xother) <= y) and (x <= int(yother) <= y)) for (xother, yother, zother) in WA]) + sum([((x <= int(xother) <= y) and (x <= int(yother) <= y)) for (xother, yother, zother) in TW])
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126 if mutations_in_motif > 0:
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127 WRCYCount[ID] += 1.0 / in_other_motifs if in_other_motifs > 0 else 1
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128 if in_other_motifs > 1:
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129 print in_other_motifs, ID, "WRCY", x, y
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28
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130
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131 for (x,y,z) in WA: #WACount[ID] = sum([1 for (x,y,z) in WA if z and z != "CDR3" and any([(x <= int(where) <= y) for (frm, where, to, a,b,c,d) in mutationdic[ID + "_" + z]])])
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132 if not z or z == "CDR3":
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133 continue
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30
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134 mutations_in_motif = sum([(x <= int(where) <= y) for (frm, where, to, a,b,c,d) in mutationdic[ID + "_" + z]])
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135 in_other_motifs = sum([((x <= int(xother) <= y) and (x <= int(yother) <= y)) for (xother, yother, zother) in RGYW]) + sum([((x <= int(xother) <= y) and (x <= int(yother) <= y)) for (xother, yother, zother) in WRCY]) + sum([((x <= int(xother) <= y) and (x <= int(yother) <= y)) for (xother, yother, zother) in TW])
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136 if mutations_in_motif > 0:
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137 WACount[ID] += 1.0 / in_other_motifs if in_other_motifs > 0 else 1
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138 if in_other_motifs > 1:
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139 print in_other_motifs, ID, "WA", x, y
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28
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140
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141 for (x,y,z) in TW: #TWCount[ID] = sum([1 for (x,y,z) in TW if z and z != "CDR3" and any([(x <= int(where) <= y) for (frm, where, to, a,b,c,d) in mutationdic[ID + "_" + z]])])
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142 if not z or z == "CDR3":
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143 continue
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30
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144 mutations_in_motif = sum([(x <= int(where) <= y) for (frm, where, to, a,b,c,d) in mutationdic[ID + "_" + z]])
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145 in_other_motifs = sum([((x <= int(xother) <= y) and (x <= int(yother) <= y)) for (xother, yother, zother) in RGYW]) + sum([((x <= int(xother) <= y) and (x <= int(yother) <= y)) for (xother, yother, zother) in WRCY]) + sum([((x <= int(xother) <= y) and (x <= int(yother) <= y)) for (xother, yother, zother) in WA])
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146 if mutations_in_motif > 0:
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147 TWCount[ID] += 1.0 / in_other_motifs if in_other_motifs > 0 else 1
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148 if in_other_motifs > 1:
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149 print in_other_motifs, ID, "TW", x, y
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0
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150
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151
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152 directory = outfile[:outfile.rfind("/") + 1]
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22
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153 value = 0
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154 valuedic = dict()
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155 for gene in genes:
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156 with open(directory + gene + "_value.txt", 'r') as v:
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157 valuedic[gene] = int(v.readlines()[0].rstrip())
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158 with open(directory + "total_value.txt", 'r') as v:
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159 valuedic["total"] = int(v.readlines()[0].rstrip())
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160
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161 dic = {"RGYW": RGYWCount, "WRCY": WRCYCount, "WA": WACount, "TW": TWCount}
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162 arr = ["RGYW", "WRCY", "WA", "TW"]
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163 with open(outfile, 'w') as o:
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164 for typ in arr:
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165 o.write(typ + " (%)")
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166 curr = dic[typ]
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167 for gene in genes:
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168 geneMatcher = re.compile(".*" + gene + ".*")
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169 if valuedic[gene] is 0:
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170 o.write(",0,0,0")
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171 else:
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172 x = int(round(sum([curr[x] for x in [y for y,z in genedic.iteritems() if geneMatcher.match(z)]])))
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173 y = valuedic[gene]
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174 z = str(round(x / float(valuedic[gene]) * 100, 1))
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175 o.write("," + str(x) + "," + str(y) + "," + z)
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176 #for total
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177 x = int(round(sum([y for x,y in curr.iteritems()])))
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178 y = valuedic["total"]
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179 z = str(round(x / float(valuedic["total"]) * 100, 1))
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180 o.write("," + str(x) + "," + str(y) + "," + z + "\n")
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21
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181
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182
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183 #for testing
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184 seq_motif_file = outfile[:outfile.rindex("/")] + "/motif_per_seq.txt"
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185 first = True
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186 with open(seq_motif_file, 'w') as o:
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187 for ID in IDlist:
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188 if first:
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189 o.write("ID\tRGYWC\tWRCY\tWA\tTW\n")
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190 first = False
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191 continue
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192 o.write(ID + "\t" + str(RGYWCount[ID]) + "\t" + str(WRCYCount[ID]) + "\t" + str(WACount[ID]) + "\t" + str(TWCount[ID]) + "\n")
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