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1 import re
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2 import argparse
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3
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4 parser = argparse.ArgumentParser()
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39
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5 parser.add_argument("--input",
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6 help="The '7_V-REGION-mutation-and-AA-change-table' and '10_V-REGION-mutation-hotspots' merged together, with an added 'best_match' annotation")
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4
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7 parser.add_argument("--genes", help="The genes available in the 'best_match' column")
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31
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8 parser.add_argument("--includefr1", help="The genes available in the 'best_match' column")
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9 parser.add_argument("--output", help="Output file")
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10
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11 args = parser.parse_args()
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12
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4
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13 infile = args.input
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14 genes = str(args.genes).split(",")
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32
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15 print "includefr1 =", args.includefr1
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31
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16 include_fr1 = True if args.includefr1 == "yes" else False
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39
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17 outfile = args.output
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4
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18
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19 genedic = dict()
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0
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20
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21 mutationdic = dict()
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22 mutationMatcher = re.compile("^(.)(\d+).(.),?(.)?(\d+)?.?(.)?(.?.?.?.?.?)?")
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23 linecount = 0
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24
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4
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25 IDIndex = 0
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26 best_matchIndex = 0
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27 fr1Index = 0
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28 cdr1Index = 0
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29 fr2Index = 0
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30 cdr2Index = 0
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31 fr3Index = 0
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39
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32 first = True
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26
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33 IDlist = []
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34 mutationList = []
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35
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4
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36 with open(infile, 'r') as i:
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39
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37 for line in i:
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38 if first:
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39 linesplt = line.split("\t")
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40 IDIndex = linesplt.index("Sequence.ID")
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41 best_matchIndex = linesplt.index("best_match")
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42 fr1Index = linesplt.index("FR1.IMGT")
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43 cdr1Index = linesplt.index("CDR1.IMGT")
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44 fr2Index = linesplt.index("FR2.IMGT")
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45 cdr2Index = linesplt.index("CDR2.IMGT")
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46 fr3Index = linesplt.index("FR3.IMGT")
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47 first = False
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48 continue
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49 linecount += 1
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50 linesplt = line.split("\t")
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51 ID = linesplt[IDIndex]
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52 genedic[ID] = linesplt[best_matchIndex]
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53 mutationdic[ID + "_FR1"] = [mutationMatcher.match(x).groups() for x in linesplt[fr1Index].split("|") if
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54 x] if include_fr1 else []
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55 mutationdic[ID + "_CDR1"] = [mutationMatcher.match(x).groups() for x in linesplt[cdr1Index].split("|") if x]
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56 mutationdic[ID + "_FR2"] = [mutationMatcher.match(x).groups() for x in linesplt[fr2Index].split("|") if x]
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57 mutationdic[ID + "_CDR2"] = [mutationMatcher.match(x).groups() for x in linesplt[cdr2Index].split("|") if x]
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58 mutationdic[ID + "_FR2-CDR2"] = mutationdic[ID + "_FR2"] + mutationdic[ID + "_CDR2"]
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59 mutationdic[ID + "_FR3"] = [mutationMatcher.match(x).groups() for x in linesplt[fr3Index].split("|") if x]
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26
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60
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61 mutationList += mutationdic[ID + "_FR1"] + mutationdic[ID + "_CDR1"] + mutationdic[ID + "_FR2"] + mutationdic[
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62 ID + "_CDR2"] + mutationdic[ID + "_FR3"]
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26
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63
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64 IDlist += [ID]
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65
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66 AA_mutation = [0] * (int(max(mutationList, key=lambda i: int(i[4]) if i[4] else 0)[
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67 4]) + 1) # [4] is the position of the AA mutation, None if silent
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26
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68
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69 for mutation in mutationList:
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39
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70 if mutation[4]: # if non silent mutation
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71 AA_mutation[int(mutation[4])] += 1
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26
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72
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73 aa_mutations_file = outfile[:outfile.rindex("/")] + "/aa_mutations.txt"
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74 with open(aa_mutations_file, 'w') as o:
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75 o.write(",".join([str(x) for x in AA_mutation]) + "\n")
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76
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0
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77 if linecount == 0:
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78 print "No data, exiting"
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79 with open(outfile, 'w') as o:
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80 o.write("RGYW (%)," + ("0,0,0\n" * len(genes)))
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81 o.write("WRCY (%)," + ("0,0,0\n" * len(genes)))
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82 o.write("WA (%)," + ("0,0,0\n" * len(genes)))
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83 o.write("TW (%)," + ("0,0,0\n" * len(genes)))
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84 import sys
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85
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86 sys.exit()
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0
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87
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88 hotspotMatcher = re.compile("[actg]+,(\d+)-(\d+)\((.*)\)")
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4
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89 RGYWCount = {g: 0 for g in genes}
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90 WRCYCount = {g: 0 for g in genes}
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91 WACount = {g: 0 for g in genes}
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92 TWCount = {g: 0 for g in genes}
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0
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93
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4
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94 IDIndex = 0
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95 ataIndex = 0
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96 tatIndex = 0
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97 aggctatIndex = 0
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98 atagcctIndex = 0
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99 first = True
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100 with open(infile, 'r') as i:
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39
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101 for line in i:
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102 if first:
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103 linesplt = line.split("\t")
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104 ataIndex = linesplt.index("X.a.t.a")
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105 tatIndex = linesplt.index("t.a.t.")
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106 aggctatIndex = linesplt.index("X.a.g.g.c.t..a.t.")
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107 atagcctIndex = linesplt.index("X.a.t..a.g.c.c.t.")
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108 first = False
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109 continue
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110 linesplt = line.split("\t")
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111 gene = linesplt[best_matchIndex]
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112 ID = linesplt[IDIndex]
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113 RGYW = [(int(x), int(y), z) for (x, y, z) in
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114 [hotspotMatcher.match(x).groups() for x in linesplt[aggctatIndex].split("|") if x]]
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115 WRCY = [(int(x), int(y), z) for (x, y, z) in
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116 [hotspotMatcher.match(x).groups() for x in linesplt[atagcctIndex].split("|") if x]]
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117 WA = [(int(x), int(y), z) for (x, y, z) in
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118 [hotspotMatcher.match(x).groups() for x in linesplt[ataIndex].split("|") if x]]
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119 TW = [(int(x), int(y), z) for (x, y, z) in
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120 [hotspotMatcher.match(x).groups() for x in linesplt[tatIndex].split("|") if x]]
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121 RGYWCount[ID], WRCYCount[ID], WACount[ID], TWCount[ID] = 0, 0, 0, 0
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122
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123 mutationList = (mutationdic[ID + "_FR1"] if include_fr1 else []) + mutationdic[ID + "_CDR1"] + mutationdic[
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124 ID + "_FR2"] + mutationdic[ID + "_CDR2"] + mutationdic[ID + "_FR3"]
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125 for mutation in mutationList:
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126 frm, where, to, AAfrm, AAwhere, AAto, junk = mutation
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127 mutation_in_RGYW = any([(start <= int(where) <= end) for (start, end, region) in RGYW])
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128 mutation_in_WRCY = any([(start <= int(where) <= end) for (start, end, region) in WRCY])
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129 mutation_in_WA = any([(start <= int(where) <= end) for (start, end, region) in WA])
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130 mutation_in_TW = any([(start <= int(where) <= end) for (start, end, region) in TW])
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131
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132 in_how_many_motifs = sum([mutation_in_RGYW, mutation_in_WRCY, mutation_in_WA, mutation_in_TW])
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133
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134 if in_how_many_motifs > 0:
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135 RGYWCount[ID] += (1.0 * int(mutation_in_RGYW)) / in_how_many_motifs
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136 WRCYCount[ID] += (1.0 * int(mutation_in_WRCY)) / in_how_many_motifs
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137 WACount[ID] += (1.0 * int(mutation_in_WA)) / in_how_many_motifs
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138 TWCount[ID] += (1.0 * int(mutation_in_TW)) / in_how_many_motifs
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0
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139
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4
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140 directory = outfile[:outfile.rfind("/") + 1]
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22
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141 value = 0
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142 valuedic = dict()
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143 for gene in genes:
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144 with open(directory + gene + "_value.txt", 'r') as v:
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145 valuedic[gene] = int(v.readlines()[0].rstrip())
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146 with open(directory + "total_value.txt", 'r') as v:
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147 valuedic["total"] = int(v.readlines()[0].rstrip())
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148
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149 dic = {"RGYW": RGYWCount, "WRCY": WRCYCount, "WA": WACount, "TW": TWCount}
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150 arr = ["RGYW", "WRCY", "WA", "TW"]
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39
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151 with open(outfile, 'w') as o:
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152 for typ in arr:
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153 o.write(typ + " (%)")
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154 curr = dic[typ]
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155 for gene in genes:
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156 geneMatcher = re.compile(".*" + gene + ".*")
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157 if valuedic[gene] is 0:
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158 o.write(",0,0,0")
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159 else:
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160 x = int(round(sum([curr[x] for x in [y for y, z in genedic.iteritems() if geneMatcher.match(z)]])))
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161 y = valuedic[gene]
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162 z = str(round(x / float(valuedic[gene]) * 100, 1))
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163 o.write("," + str(x) + "," + str(y) + "," + z)
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164 # for total
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165 x = int(round(sum([y for x, y in curr.iteritems()])))
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166 y = valuedic["total"]
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167 z = str(round(x / float(valuedic["total"]) * 100, 1))
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168 o.write("," + str(x) + "," + str(y) + "," + z + "\n")
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169
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170
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39
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171 # for testing
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172 seq_motif_file = outfile[:outfile.rindex("/")] + "/motif_per_seq.txt"
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173 with open(seq_motif_file, 'w') as o:
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174 o.write("ID\tRGYWC\tWRCY\tWA\tTW\n")
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175 for ID in IDlist:
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176 o.write(ID + "\t" + str(round(RGYWCount[ID], 2)) + "\t" + str(round(WRCYCount[ID], 2)) + "\t" + str(
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177 round(WACount[ID], 2)) + "\t" + str(round(TWCount[ID], 2)) + "\n")
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