|
0
|
1 <tool id="interproscan" name="Interproscan functional predictions of ORFs" version="5.0.0">
|
|
|
2 <description>Interproscan functional predictions of ORFs</description>
|
|
|
3 <requirements>
|
|
|
4 <requirement type="package">signalp</requirement>
|
|
|
5 <requirement type="package">phobius</requirement>
|
|
|
6 <requirement type="package">tmhmm</requirement>
|
|
|
7 <requirement type="set_environment">INTERPROSCAN_SCRIPT_PATH</requirement>
|
|
|
8 </requirements>
|
|
|
9 <command>
|
|
|
10 #import os
|
|
|
11 interproscan.sh -dp --fasta $infile --seqtype $seqtype -f $oformat --applications $appl --tempdir \$TEMP
|
|
|
12
|
|
|
13 $pathways
|
|
|
14 $goterms
|
|
|
15 $iprlookup
|
|
|
16
|
|
|
17 #if str($oformat) in ['SVG', 'HTML']:
|
|
|
18 --output-file-base $outfile
|
|
|
19 2>&1;
|
|
|
20 mkdir -p $outfile.files_path;
|
|
|
21 #set temp_archive_file = str($outfile) + '.' + str($oformat).lower() + '.tar.gz'
|
|
|
22 tar -C $outfile.files_path -xvmzf $temp_archive_file;
|
|
|
23 python \$INTERPROSCAN_SCRIPT_PATH/create_index.py $outfile $outfile.files_path;
|
|
|
24 rm $temp_archive_file
|
|
|
25 #else:
|
|
|
26 -o $outfile
|
|
|
27 2>&1
|
|
|
28 #end if
|
|
|
29
|
|
|
30 </command>
|
|
|
31 <inputs>
|
|
|
32 <param name="infile" type="data" format="fasta" label="Protein Fasta File"/>
|
|
|
33
|
|
|
34 <param name="seqtype" type="select" label="Type of the input sequences" help="">
|
|
|
35 <option value="p" selected="true">Protein</option>
|
|
|
36 <option value="n">DNA / RNA</option>
|
|
|
37 </param>
|
|
|
38
|
|
|
39 <param name="appl" type="select" multiple="True" display="checkboxes" label="Applications to run" help="Select your programm.">
|
|
|
40 <option value="TIGRFAM" selected="true">TIGRFAM: protein families based on Hidden Markov Models or HMMs</option>
|
|
|
41 <option value="PIRSF" selected="true">PIRSF: non-overlapping clustering of UniProtKB sequences into a hierarchical order (evolutionary relationships)</option>
|
|
|
42 <option value="ProDom" selected="true">ProDom: set of protein domain families generated from the UniProtKB</option>
|
|
|
43 <option value="Panther" selected="true">Panther: Protein ANalysis THrough Evolutionary Relationships</option>
|
|
|
44 <option value="SMART" selected="true">SMART: identification and analysis of domain architectures based on Hidden Markov Models or HMMs</option>
|
|
|
45 <option value="PrositeProfiles" selected="true">PROSITE Profiles: protein domains, families and functional sites as well as associated profiles to identify them</option>
|
|
|
46 <option value="PrositePatterns" selected="true">PROSITE Pattern: protein domains, families and functional sites as well as associated patterns to identify them</option>
|
|
|
47 <option value="HAMAP" selected="true">HAMAP: High-quality Automated Annotation of Microbial Proteomes</option>
|
|
|
48 <option value="PfamA" selected="true">PfamA: protein families, each represented by multiple sequence alignments and hidden Markov models</option>
|
|
|
49 <option value="PRINTS" selected="true">PRINTS: group of conserved motifs (fingerprints) used to characterise a protein family</option>
|
|
|
50 <option value="SuperFamily" selected="true">SUPERFAMILY: database of structural and functional annotation</option>
|
|
|
51 <option value="Coils" selected="true">Coils: Prediction of Coiled Coil Regions in Proteins</option>
|
|
|
52 <option value="Gene3d" selected="true">Gene3d: Structural assignment for whole genes and genomes using the CATH domain structure database</option>
|
|
|
53 <option value="SignalP-GRAM_POSITIVE" selected="false">SignalP Gram Positive Bacteria</option>
|
|
|
54 <option value="SignalP-GRAM_NEGATIVE" selected="false">SignalP Gram Negative Bacteria</option>
|
|
|
55 <option value="SignalP-EUK" selected="true">SignalP Eukaryotic Bacteria</option>
|
|
|
56 <option value="Phobius" selected="true">Phobius: combined transmembrane topology and signal peptide predictor</option>
|
|
|
57 <option value="TMHMM" selected="true">TMHMM: Prediction of transmembrane helices in proteins</option>
|
|
|
58 </param>
|
|
|
59
|
|
|
60 <param name="pathways" truevalue="--pathways" falsevalue="" checked="True" type="boolean" label="Include pathway information" help="Option that provides mappings from matches to pathway information, which is based on the matched manually curated InterPro entries."/>
|
|
|
61 <param name="goterms" truevalue="--goterms" falsevalue="" checked="True" type="boolean" label="Include Gene Ontology (GO) mappings" />
|
|
|
62 <param name="iprlookup" truevalue="--iprlookup" falsevalue="" checked="False" type="boolean" label="Provide mappings from matched member database signatures to the InterPro entries that they are integrated into" />
|
|
|
63
|
|
|
64 <param name="oformat" type="select" label="Output format" help="Please select a output format.">
|
|
|
65 <option value="TSV" selected="true">Tab-separated values format (TSV)</option>
|
|
|
66 <option value="GFF3">GFF3</option>
|
|
|
67 <option value="SVG">SVG</option>
|
|
|
68 <option value="HTML">HTML</option>
|
|
|
69 <option value="XML">XML</option>
|
|
|
70 </param>
|
|
|
71
|
|
|
72 </inputs>
|
|
|
73 <outputs>
|
|
|
74
|
|
|
75 <data format="tabular" name="outfile" label="Interproscan calculation on ${on_string}">
|
|
|
76 <change_format>
|
|
|
77 <when input="oformat" value="HTML" format="html"/>
|
|
|
78 <when input="oformat" value="XML" format="xml"/>
|
|
|
79 <when input="oformat" value="SVG" format="html"/>
|
|
|
80 <when input="oformat" value="GFF3" format="gff"/>
|
|
|
81 </change_format>
|
|
|
82 </data>
|
|
|
83
|
|
|
84 </outputs>
|
|
|
85 <requirements>
|
|
|
86 </requirements>
|
|
|
87 <help>
|
|
|
88
|
|
|
89 **What it does**
|
|
|
90
|
|
|
91 Interproscan is a batch tool to query the Interpro database. It provides annotations based on multiple searches of profile and other functional databases.
|
|
|
92
|
|
|
93
|
|
|
94 #####
|
|
|
95 Input
|
|
|
96 #####
|
|
|
97
|
|
|
98 Required is a FASTA file containing protein or nucleotide sequences.
|
|
|
99
|
|
|
100
|
|
|
101 ######
|
|
|
102 Output
|
|
|
103 ######
|
|
|
104
|
|
|
105 In this version of InterProScan_, you can retrieve output in any of the following five formats:
|
|
|
106
|
|
|
107 * TSV: a simple tab-delimited file format
|
|
|
108 * XML: the new "IMPACT" XML format (XSD available here_).
|
|
|
109 * GFF: The `GFF 3.0`_ format
|
|
|
110 * HTML: An HTML representation of the protein matches
|
|
|
111 * SVG: An Scalable Vector Graphics representation of the protein matches
|
|
|
112
|
|
|
113
|
|
|
114 .. _`GFF3 3.0`: http://gmod.org/wiki/GFF#GFF3_Format
|
|
|
115 .. _here: http://www.ebi.ac.uk/interpro/resources/schemas/interproscan5
|
|
|
116
|
|
|
117
|
|
|
118 Previous versions of InterProScan_ 5 (I5RC1 - I5RC6) could only output data for nucleotide sequences in XML and GFF3. This has now changed and InterProScan_ 5 can output results for nucleotide sequences in all formats. *Please note* you can only trace protein match positions to the original nucleotide sequence with GFF3 and XML.
|
|
|
119
|
|
|
120 You can override the default output formats using the *-f* option, e.g.::
|
|
|
121
|
|
|
122 ./interproscan.sh -f XML -f HTML -i /path/to/sequences.fasta -b /path/to/output_file
|
|
|
123
|
|
|
124 or
|
|
|
125
|
|
|
126 ::
|
|
|
127
|
|
|
128 ./interproscan.sh -f XML, HTML -i /path/to/sequences.fasta -b /path/to/output_file
|
|
|
129
|
|
|
130
|
|
|
131 These two equivalent commands will output the results in XML and HTML format.
|
|
|
132
|
|
|
133 Tab-separated values format (TSV)
|
|
|
134 =================================
|
|
|
135
|
|
|
136 Basic tab delimited format.
|
|
|
137
|
|
|
138
|
|
|
139 Example Output
|
|
|
140 --------------
|
|
|
141
|
|
|
142 ::
|
|
|
143
|
|
|
144 P51587 14086411a2cdf1c4cba63020e1622579 3418 Pfam PF09103 BRCA2, oligonucleotide/oligosaccharide-binding, domain 1 2670 2799 7.9E-43 T 15-03-2013
|
|
|
145 P51587 14086411a2cdf1c4cba63020e1622579 3418 ProSiteProfiles PS50138 BRCA2 repeat profile. 1002 1036 0.0 T 18-03-2013 IPR002093 BRCA2 repeat GO:0005515|GO:0006302
|
|
|
146 P51587 14086411a2cdf1c4cba63020e1622579 3418 Gene3D G3DSA:2.40.50.140 2966 3051 3.1E-52 T 15-03-2013
|
|
|
147 ...
|
|
|
148
|
|
|
149
|
|
|
150 The TSV format presents the match data in columns as follows:
|
|
|
151
|
|
|
152 - Protein Accession (e.g. P51587)
|
|
|
153 - Sequence MD5 digest (e.g. 14086411a2cdf1c4cba63020e1622579)
|
|
|
154 - Sequence Length (e.g. 3418)
|
|
|
155 - Analysis (e.g. Pfam / PRINTS / Gene3D)
|
|
|
156 - Signature Accession (e.g. PF09103 / G3DSA:2.40.50.140)
|
|
|
157 - Signature Description (e.g. BRCA2 repeat profile)
|
|
|
158 - Start location
|
|
|
159 - Stop location
|
|
|
160 - Score - is the e-value of the match reported by member database method (e.g. 3.1E-52)
|
|
|
161 - Status - is the status of the match (T: true)
|
|
|
162 - Date - is the date of the run
|
|
|
163 - (InterProScan_ annotations - accession (e.g. IPR002093) - optional column; only displayed if -iprscan option is switched on)
|
|
|
164 - (InterProScan_ annotations - description (e.g. BRCA2 repeat) - optional column; only displayed if -iprscan option is switched on)
|
|
|
165 - (GO annotations (e.g. GO:0005515) - optional column; only displayed if --goterms option is switched on)
|
|
|
166 - (Pathways annotations (e.g. REACT_71) - optional column; only displayed if --pathways option is switched on)
|
|
|
167
|
|
|
168
|
|
|
169 Extensible Markup Language (XML)
|
|
|
170 ================================
|
|
|
171
|
|
|
172 XML representation of the matches - this is the richest form of the data. The XML Schema Definition (XSD) is available [http://www.ebi.ac.uk/interpro/resources/schemas/interproscan5 here].
|
|
|
173
|
|
|
174 Example Output
|
|
|
175 --------------
|
|
|
176
|
|
|
177 ::
|
|
|
178
|
|
|
179 .. image:: $PATH_TO_IMAGES/example_xml_output.png
|
|
|
180
|
|
|
181
|
|
|
182
|
|
|
183 Generic Feature Format Version 3 (GFF3)
|
|
|
184 =======================================
|
|
|
185
|
|
|
186 The GFF3 format is a flat tab-delimited file, which is much richer then the TSV output format. It allows you to trace back from matches to predicted proteins and to nucleic acid sequences. It also contains a FASTA format representation of the predicted protein sequences and their matches. You will find a documentation of all the columns and attributes used on [http://www.sequenceontology.org/gff3.shtml].
|
|
|
187
|
|
|
188 Example Output
|
|
|
189 --------------
|
|
|
190
|
|
|
191 ::
|
|
|
192
|
|
|
193 ##gff-version 3
|
|
|
194 ##feature-ontology http://song.cvs.sourceforge.net/viewvc/song/ontology/sofa.obo?revision=1.269
|
|
|
195 ##sequence-region AACH01000027 1 1347
|
|
|
196 ##seqid|source|type|start|end|score|strand|phase|attributes
|
|
|
197 AACH01000027 provided_by_user nucleic_acid 1 1347 . + . Name=AACH01000027;md5=b2a7416cb92565c004becb7510f46840;ID=AACH01000027
|
|
|
198 AACH01000027 getorf ORF 1 1347 . + . Name=AACH01000027.2_21;Target=pep_AACH01000027_1_1347 1 449;md5=b2a7416cb92565c004becb7510f46840;ID=orf_AACH01000027_1_1347
|
|
|
199 AACH01000027 getorf polypeptide 1 449 . + . md5=fd0743a673ac69fb6e5c67a48f264dd5;ID=pep_AACH01000027_1_1347
|
|
|
200 AACH01000027 Pfam protein_match 84 314 1.2E-45 + . Name=PF00696;signature_desc=Amino acid kinase family;Target=null 84 314;status=T;ID=match$8_84_314;Ontology_term="GO:0008652";date=15-04-2013;Dbxref="InterPro:IPR001048","Reactome:REACT_13"
|
|
|
201 ##sequence-region 2
|
|
|
202 ...
|
|
|
203 >pep_AACH01000027_1_1347
|
|
|
204 LVLLAAFDCIDDTKLVKQIIISEIINSLPNIVNDKYGRKVLLYLLSPRDPAHTVREIIEV
|
|
|
205 LQKGDGNAHSKKDTEIRRREMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEA
|
|
|
206 GHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQI
|
|
|
207 LLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQ
|
|
|
208 ADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIKAA
|
|
|
209 TIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQGSIWV
|
|
|
210 DKGAAEALSQYGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKGRVQFGASALEDML
|
|
|
211 RSQKAKGVLIYRDDWISITPEIQLLFTEF
|
|
|
212 ...
|
|
|
213 >match$8_84_314
|
|
|
214 KRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHELILVSSGAIAAGFGALGFKK
|
|
|
215 RPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQILLTQDDFVDKRRYKNAHQALSVL
|
|
|
216 LNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQADLLVFLTDVDGLYTGNPNSDPR
|
|
|
217 AKRLERIETINREIIDMAGGAGSSNGTGGMLTKIKAATIATESGVPVYICS
|
|
|
218
|
|
|
219
|
|
|
220 Scalable Vector Graphics (SVG) and HyperText Markup Language (HTML)
|
|
|
221 ====================================================================
|
|
|
222
|
|
|
223 InterProScan_ 5 outputs a single HTML/SVG file for each protein sequence analysed. The HTML/SVG file(s) are compressed into a single gzipped tar archive (or "tarball") that includes the resources (images, Javascript, style etc) to render the pages/images in a browser or image viewer. (Note that from version 5RC4, the SVG format has no external dependencies.)
|
|
|
224
|
|
|
225 The tarball will be named something similar to::
|
|
|
226
|
|
|
227 base_output_file_name.html.tar.gz OR base_output_file_name.svg.tar.gz
|
|
|
228
|
|
|
229
|
|
|
230 To access the HTML pages/SVG images, unzip the tarball using a command like:
|
|
|
231
|
|
|
232 tar -xvzf base_output_file_name.html(svg).tar.gz
|
|
|
233
|
|
|
234 You can then open the unzipped HTML/SVG files in any browser or image viewer (for SVG).
|
|
|
235
|
|
|
236 Example Output
|
|
|
237 --------------
|
|
|
238
|
|
|
239 .. image:: $PATH_TO_IMAGES/P51587.svg.png
|
|
|
240
|
|
|
241 .. _InterProScan: http://www.ebi.ac.uk/interpro
|
|
|
242
|
|
|
243
|
|
|
244 ----------
|
|
|
245 References
|
|
|
246 ----------
|
|
|
247
|
|
|
248 Zdobnov EM, Apweiler R (2001)
|
|
|
249 InterProScan an integration platform for the signature-recognition methods in InterPro.
|
|
|
250 Bioinformatics 17, 847-848.
|
|
|
251 http://dx.doi.org/10.1093/bioinformatics/17.9.847
|
|
|
252
|
|
|
253 Quevillon E, Silventoinen V, Pillai S, Harte N, Mulder N, Apweiler R, Lopez R (2005)
|
|
|
254 InterProScan: protein domains identifier.
|
|
|
255 Nucleic Acids Research 33 (Web Server issue), W116-W120.
|
|
|
256 http://dx.doi.org/10.1093/nar/gki442
|
|
|
257
|
|
|
258 Hunter S, Apweiler R, Attwood TK, Bairoch A, Bateman A, Binns D, Bork P, Das U, Daugherty L, Duquenne L, Finn RD, Gough J, Haft D, Hulo N, Kahn D, Kelly E, Laugraud A, Letunic I, Lonsdale D, Lopez R, Madera M, Maslen J, McAnulla C, McDowall J, Mistry J, Mitchell A, Mulder N, Natale D, Orengo C, Quinn AF, Selengut JD, Sigrist CJ, Thimma M, Thomas PD, Valentin F, Wilson D, Wu CH, Yeats C. (2009)
|
|
|
259 InterPro: the integrative protein signature database.
|
|
|
260 Nucleic Acids Research 37 (Database Issue), D224-228.
|
|
|
261 http://dx.doi.org/10.1093/nar/gkn785
|
|
|
262
|
|
|
263
|
|
|
264 This wrapper is available to install into other Galaxy Instances via the Galaxy Tool Shed at
|
|
|
265 http://toolshed.g2.bx.psu.edu/view/bgruening/interproscan
|
|
|
266
|
|
|
267
|
|
|
268 **Galaxy Wrapper Author**::
|
|
|
269
|
|
|
270 * Bjoern Gruening, Pharmaceutical Bioinformatics, University of Freiburg
|
|
|
271 * Konrad Paszkiewicz, Exeter Sequencing Service, University of Exeter
|
|
|
272
|
|
|
273 </help>
|
|
|
274 </tool>
|
