Mercurial > repos > bgruening > deeptools_compute_matrix

annotate computeMatrix.xml @ 27:459189886f08 draft

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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
author bgruening
date Fri, 31 Mar 2017 08:26:05 -0400
parents 10929984ab48
children 2f7094a2cfd5
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1 <tool id="deeptools_compute_matrix" name="computeMatrix" version="@WRAPPER_VERSION@.0">
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2 <description>prepares data for plotting a heatmap or a profile of given regions</description>
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3 <macros>
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4 <token name="@BINARY@">computeMatrix</token>
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5 <import>deepTools_macros.xml</import>
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6 </macros>
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7 <expand macro="requirements" />
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8 <command>
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9 <![CDATA[
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10 #import re
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11 #import tempfile
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12 #set bw_files=[]
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13 #for $counter, $bigwig, in enumerate($scoreFileName):
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14 #set identifier = re.sub('[^\s\w\-]', '_', str($bigwig.element_identifier))
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15 ln -f -s "${bigwig}" "${identifier}_${counter}.bw" &&
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16 #silent $bw_files.append('%s_%s.bw' % ($identifier, $counter))
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17 #end for
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19 #set bed_files=[]
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20 #for $counter, $rf, in enumerate($regionsFiles):
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21 #set identifier = re.sub('[^\s\w\-]', '_', str($rf.regionsFile.element_identifier))
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22 ln -f -s "${rf.regionsFile}" "${identifier}_${counter}.bed" &&
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23 #silent $bed_files.append('%s_%s.bed' % ($identifier, $counter))
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24 #end for
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26 @BINARY@
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28 $mode.mode_select
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29 --regionsFileName '#echo "' '".join($bed_files)#'
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31 --scoreFileName '#echo "' '".join($bw_files)#'
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32
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33 --outFileName '$outFileName'
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35 @THREADS@
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37 #if $output.showOutputSettings == "yes"
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38 #if $output.saveMatrix:
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39 --outFileNameMatrix '$outFileNameMatrix'
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40 #end if
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42 #if $output.saveSortedRegions:
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43 --outFileSortedRegions '$outFileSortedRegions'
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44 #end if
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45 #end if
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47 #if $mode.mode_select == "reference-point":
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48 --referencePoint $mode.referencePoint
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49 $mode.nanAfterEnd
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50 --beforeRegionStartLength $mode.beforeRegionStartLength
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51 --afterRegionStartLength $mode.afterRegionStartLength
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52 #else
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53 --regionBodyLength $mode.regionBodyLength
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54 #if $mode.regionStartLength.regionStartLength_select == "yes":
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55 --beforeRegionStartLength $mode.regionStartLength.beforeRegionStartLength
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56 --afterRegionStartLength $mode.regionStartLength.afterRegionStartLength
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58 --unscaled5prime $mode.regionStartLength.unscaled5prime
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59 --unscaled3prime $mode.regionStartLength.unscaled3prime
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60
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61 #end if
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63 #end if
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65 #if $advancedOpt.showAdvancedOpt == "yes":
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66 --sortRegions '$advancedOpt.sortRegions'
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67 --sortUsing '$advancedOpt.sortUsing'
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68 --averageTypeBins '$advancedOpt.averageTypeBins'
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69 $advancedOpt.skipZeros
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70 $advancedOpt.missingDataAsZero
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71 --binSize $advancedOpt.binSize
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73 #if $advancedOpt.minThreshold is not None and str($advancedOpt.minThreshold) != '':
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74 --minThreshold $advancedOpt.minThreshold
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75 #end if
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76 #if $advancedOpt.maxThreshold is not None and str($advancedOpt.maxThreshold) != '':
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77 --maxThreshold $advancedOpt.maxThreshold
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78 #end if
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79 #if $advancedOpt.scale is not None and str($advancedOpt.scale) != '':
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80 --scale $advancedOpt.scale
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81 #end if
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83 @ADVANCED_OPTS_GTF@
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85 @blacklist@
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86
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87 #end if
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88 ]]>
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89 </command>
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90 <inputs>
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91
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92 <repeat name="regionsFiles" title="Select regions" min="1">
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93 <param name="regionsFile" format="bed,gtf" type="data" label="Regions to plot"
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94 help="File, in BED format, containing the regions to plot."/>
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95 </repeat>
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96
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97 <param name="scoreFileName" format="bigwig" type="data"
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98 label="Score file" multiple="True"
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99 help="You can generate a bigWig file from a BAM file using the
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100 bamCoverage tool. (--scoreFileName)"/>
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101
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102 <conditional name="mode" >
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103 <param name="mode_select" type="select"
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104 label="computeMatrix has two main output options"
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105 help="In the scale-regions mode, all regions in the BED file are
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106 stretched or shrunken to the same length (in bases) that is indicated
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107 by the user. Reference-point refers to a position within the BED
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108 regions (start or end of each region). In the reference-point mode only
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109 those genomic positions before (upstream) and/or after (downstream)
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110 the reference point will be considered.">
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111 <option value="scale-regions" selected="true">scale-regions</option>
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112 <option value="reference-point">reference-point</option>
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113 </param>
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114
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115 <when value="scale-regions" >
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116 <param argument="--regionBodyLength" type="integer" value="500"
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117 label="Distance in bases to which all regions are going to be fit" help=""/>
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118 <conditional name="regionStartLength">
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119 <param name="regionStartLength_select" type="select" label="Set distance up- and downstream of the given regions">
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120 <option value="no" selected="true">no</option>
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121 <option value="yes">yes</option>
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122 </param>
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123 <when value="no" />
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124 <when value="yes">
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125 <param argument="--beforeRegionStartLength" type="integer" value="1000" min="1"
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126 label="Distance upstream of the region start position"
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127 help="If the regions are genes, this would be the
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128 distance upstream of the transcription start site."/>
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129 <param argument="--afterRegionStartLength" type="integer" value="1000" min="1"
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130 label="Distance downstream of the region end position"
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131 help="If the regions are genes, this would be the
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132 distance downstream of the transcription end site."/>
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133 <param argument="--unscaled5prime" type="integer" min="0" value="0"
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134 label="Number of bases within the gene body at the 5-prime end to exclude from scaling."
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135 help="By default, each region is scaled to a given length (see the --regionBodyLength
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136 option). In some cases it is useful to look at unscaled signals around region boundaries, so this
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137 setting specifies the number of unscaled bases on the 5-prime end of each boundary." />
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138 <param argument="--unscaled3prime" type="integer" min="0" value="0"
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139 label="Number of bases within the gene body at the 3-prime end to exclude from scaling."
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140 help="As with --unscaled5prime, but for the 3-prime end." />
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141 </when>
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142 </conditional>
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143 </when>
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144 <when value="reference-point">
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145 <param name="referencePoint" type="select" label="The reference point for the plotting">
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146 <option value="TSS" selected="true">beginning of region (e.g. TSS)</option>
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147 <option value="TES">end of region (e.g. TES)</option>
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148 <option value="center">center of region</option>
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149 </param>
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150 <param name="nanAfterEnd" type="boolean" truevalue="--nanAfterEnd" falsevalue=""
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151 label="Discard any values after the region end"
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152 help="This is useful to visualize the region end when not using the
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153 scale-regions mode and when the reference-point is set to the TSS. (--nanAfterEnd)"/>
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154 <param name="beforeRegionStartLength" type="integer" value="1000" min="1"
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155 label="Distance upstream of the start site of the regions defined in the region file"
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156 help="If the regions are genes, this would be the distance upstream of the transcription start site. (--beforeRegionStartLength)"/>
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157 <param name="afterRegionStartLength" type="integer" value="1000" min="1"
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158 label="Distance downstream of the end site of the given regions"
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159 help="If the regions are genes, this would be the distance downstream of the transcription end site. (--afterRegionStartLength)"/>
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160 </when>
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161 </conditional>
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162
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163 <expand macro="input_graphic_output_settings">
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164 <expand macro="input_save_matrix_values" />
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165 </expand>
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166
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167 <conditional name="advancedOpt" >
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168 <param name="showAdvancedOpt" type="select" label="Show advanced options" >
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169 <option value="no" selected="true">no</option>
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170 <option value="yes">yes</option>
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171 </param>
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172 <when value="no" />
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173 <when value="yes">
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174 <param name="binSize" type="integer" value="50" min="1"
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175 label="Length, in bases, of non-overlapping bins used for averaging the score over the regions length"
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176 help="(--binSize)"/>
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177
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178 <expand macro="sortRegionsComputeMatrix" />
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179 <expand macro="sortUsing" />
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180
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181 <param name="averageTypeBins" type="select"
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182 label="Define the type of statistic that should be displayed."
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183 help="The value is computed for each bin. (--averageTypeBins)">
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184 <option value="mean" selected="true">mean</option>
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185 <option value="median">median</option>
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186 <option value="min">min</option>
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187 <option value="max">max</option>
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188 <option value="sum">sum</option>
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189 <option value="std">std</option>
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190 </param>
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191
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192 <param name="missingDataAsZero" type="boolean" truevalue="--missingDataAsZero" falsevalue="" checked="False"
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193 label="Convert missing values to 0?"
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194 help="If set to 'yes', missing values (NAs) are converted
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195 to 0. If you want to use clustering with plotHeatmap
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196 or plotProfile, set this to 'yes'.
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197 The default is to ignore missing values, which will be
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198 depicted as black areas once a heatmap is created.
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199 (--missingDataAsZero)" />
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200
0
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201 <expand macro="skipZeros" />
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202
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203 <param name="minThreshold" type="float" optional="True"
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204 label="Minimum threshold"
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205 help="Any region containing a value that is equal or less than this numeric
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206 value will be skipped. This is useful to skip, for example, genes where the
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207 read count is zero for any of the bins which could be the result of
0
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208 unmappable areas and can bias the overall results. (--minThreshold)"/>
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209 <param name="maxThreshold" type="float" optional="True"
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210 label="Maximum threshold"
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211 help="Any region containing a value that is equal or higher that this
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212 numeric value will be skipped. The max threshold is useful to skip those
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213 few regions with very high read counts (e.g. major satellites) that may
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214 bias the average values. (--maxThreshold)"/>
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215 <param name="scale" type="float" optional="True" label="Scaling factor"
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216 help="If set, all values are multiplied by this number. (--scale)"/>
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217
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218 <expand macro="gtf_options" />
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219
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220 <expand macro="blacklist" />
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221 </when>
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222 </conditional>
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223 </inputs>
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224 <outputs>
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225 <data format="deeptools_compute_matrix_archive" name="outFileName" label="${tool.name} on ${on_string}: Matrix" />
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226 <expand macro="output_graphic_outputs" />
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227 <expand macro="output_save_matrix_values" />
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228 </outputs>
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229 <tests>
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230 <test>
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231 <param name="regionsFile" value="computeMatrix1.bed" ftype="bed" />
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232 <param name="scoreFileName" value="bamCoverage_result4.bw" ftype="bigwig" />
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233 <param name="showAdvancedOpt" value="yes" />
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234 <param name="mode_select" value="reference-point" />
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235 <param name="binSize" value="10" />
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236 <param name="sortUsing" value="sum" />
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237 <param name="averageTypeBins" value="sum" />
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238 <param name="beforeRegionStartLength" value="10" />
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239 <param name="afterRegionStartLength" value="10" />
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240 <output name="outFileName" file="computeMatrix_result1.gz" ftype="deeptools_compute_matrix_archive" compare="sim_size" />
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241 </test>
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242 <test>
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243 <param name="regionsFile" value="computeMatrix2.bed" ftype="bed" />
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244 <param name="scoreFileName" value="computeMatrix2.bw" ftype="bigwig" />
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245 <param name="showAdvancedOpt" value="yes" />
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246 <param name="mode_select" value="reference-point" />
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247 <param name="binSize" value="10" />
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248 <param name="beforeRegionStartLength" value="10" />
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249 <param name="afterRegionStartLength" value="10" />
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250 <output name="outFileName" file="computeMatrix_result2.gz" ftype="deeptools_compute_matrix_archive" compare="sim_size" />
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251 </test>
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252 <test>
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253 <param name="regionsFile" value="computeMatrix2.bed" ftype="bed" />
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254 <param name="scoreFileName" value="computeMatrix2.bw" ftype="bigwig" />
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255 <param name="showAdvancedOpt" value="yes" />
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256 <param name="mode_select" value="scale-regions" />
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257 <param name="endLabel" value="END" />
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258 <param name="regionStartLength" value="yes" />
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259 <output name="outFileName" file="computeMatrix_result3.gz" ftype="deeptools_compute_matrix_archive" compare="sim_size" />
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260 </test>
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261 </tests>
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262 <help>
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263 <![CDATA[
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264
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265 What it does
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266 ----------------
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267
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268 This tool prepares an intermediate file (a gzipped table of values)
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269 that contains scores associated with genomic regions.
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270 The regions can either be scaled to the same size (using the ``scale-regions`` mode) or you can choose the start, end, or center of each region as the focus point for the score calculations.
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271 For more details, check out the explanation `here <file:///Users/frd2007/Documents/MPI/deepTools/docs/_build/html/content/tools/computeMatrix.html#details>`_.
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272
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273 The intermediate file produced by ``computeMatrix`` is meant to be used with ``plotHeatmap`` and ``plotProfile``.
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274 See the descriptions of ``plotHeatmap`` and ``plotProfile`` for example plots.
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275
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276 .. image:: $PATH_TO_IMAGES/computeMatrix_overview.png
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277 :alt: Relationship between computeMatrix, heatmapper and profiler
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278 :width: 600
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279 :height: 418
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280
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281 =======
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282
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283 Usage hints
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284 -------------
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285
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286 The supplied genomic regions can really be anything - genes, parts of genes, ChIP-seq peaks, favorite genome regions... as long as you provide a proper file
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287 in BED or INTERVAL format. If you would like to compare different groups of regions (e.g., genes from chromosome 2 and 3), you can supply more than 1 regions file, one for each group by selecting "Insert Select regions".
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288
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289 .. image:: $PATH_TO_IMAGES/computeMatrix_selectRegions.png
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290 :width: 600
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291 :height: 150
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292
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293 You can select as many score (bigWig) files as you like. Simply use the Shift and/or Command key while clicking on the files of interest.
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294
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295 .. image:: $PATH_TO_IMAGES/computeMatrix_selectScores.png
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296 :width: 600
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297 :height: 136
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298
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299 The multitude of parameters can seem daunting at first - here are the options that we tend to tune most often:
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300
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301 * ``bin Size`` -- The default value works well most of the time, but if you want to have a more finely grained image, decrease the default value (but not smaller than your bigWig file(s)' bin size). If you want to reduce the computation time, increase it.
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302 * ``Skip zeros`` -- useful to avoid completely blank lines in the heatmap.
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303 * ``Convert missing values to 0?`` -- If you want to identify clusters of similar regions in an unsupervised fashion using ``plotHeatmap`` and/or ``plotProfile``, you should definitely set this to 'yes'.
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305
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306 Output files
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307 ---------------
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308
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309 The default output is a **gzipped table of values** that is used by both ``plotHeatmap`` and ``plotProfile``.
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310
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311 The optional output files include a) the **regions after sorting and filtering (if selected)** as they were used to calculate the values for the plotting, and b) the uncompressed table that **underlies the heatmap**.
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312
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313 **TIP:** ``computeMatrix`` can also be used to filter and sort regions according to their score by making use of the "advanced output settings".
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314
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315 .. image:: $PATH_TO_IMAGES/computeMatrix_advancedOutput.png
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316 :width: 600
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317 :height: 189
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318
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319 .. image:: $PATH_TO_IMAGES/computeMatrix_output.png
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320 :width: 600
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321 :height: 297
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322
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323 Note that these advanced output options are available for ``plotHeatmap`` and ``plotProfile``, too.
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324
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325 See the following table for the optional output options:
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326
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327 +-----------------------------------+--------------------+-----------------+-----------------+
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328 | **optional output type** | **computeMatrix** | **plotHeatmap** | **plotProfile** |
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329 +-----------------------------------+--------------------+-----------------+-----------------+
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330 | values underlying the heatmap | yes | yes | no |
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331 +-----------------------------------+--------------------+-----------------+-----------------+
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332 | values underlying the profile | no | no | yes |
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333 +-----------------------------------+--------------------+-----------------+-----------------+
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334 | sorted and/or filtered regions | yes | yes | yes |
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335 +-----------------------------------+--------------------+-----------------+-----------------+
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336
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337 **More examples** can be found in our `Gallery <http://deeptools.readthedocs.org/en/latest/content/example_gallery.html#normalized-chip-seq-signals-and-peak-regions>`_.
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338
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339 -----
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340
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341 @REFERENCES@
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342 ]]>
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343 </help>
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344 <expand macro="citations" />
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345 </tool>