Mercurial > repos > bebatut > qiime
changeset 0:c1bd0c560018 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime commit bcbe76277f3e60303faf826f8ce7f018bc663a9a-dirty
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README.rst Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,7 @@ +QIIME 1.9.1 Galaxy Wrapper +-------------------------- + +Note: Many of these tools output html files that will not display properly +unless sanitization is turned off in Galaxy. This can be done globally via the +`santize_all_html` option in `galaxy.ini` or on a per tool basis using the +`santize_whitelist_file` in `galaxy.ini`.
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/align_seqs.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,128 @@ +<tool id="qiime_align_seqs" name="Align sequences" version="1.9.1"> + <description>using a variety of alignment methods</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <version_command><![CDATA[ + align_seqs.py --version + ]]> + </version_command> + + <command> +<![CDATA[ + align_seqs.py + -i $input_fasta_fp + -o align_seqs + -m $alignment.alignment_method + + #if $alignment.alignment_method == 'pynast': + -a $alignment.pairwise_alignment_method + #end if + + #if str($template_fp) != 'None': + -t $template_fp + #end if + + #if $min_length: + -e $min_length + #end if + + -p $min_percent_id +]]> + </command> + + <inputs> + <param name="input_fasta_fp" type="data" format="fasta" + label="Input fasta file" help="(-i/--input_fasta_fp)"/> + + <conditional name="alignment"> + <param name="alignment_method" type="select" + label="Method for aligning sequences" help="(-m/--alignment_method)"> + <option value="pynast" selected="True" >pynast</option> + <option value="infernal" >infernal</option> + <option value="clustalw" >clustalw</option> + <option value="muscle" >muscle</option> + <option value="mafft" >mafft</option> + </param> + <when value="pynast"> + <param name="pairwise_alignment_method" type="select" + label="Method for performing pairwise alignment in PyNAST" + help="(-a/--pairwise_alignment_method)"> + <option value="muscle" >muscle</option> + <option value="pair_hmm" >pair_hmm</option> + <option value="clustal" >clustal</option> + <option value="blast" >blast</option> + <option value="uclust" selected="True" >uclust</option> + <option value="mafft" >mafft</option> + </param> + </when> + <when value="infernal" /> + <when value="clustalw" /> + <when value="muscle" /> + <when value="mafft" /> + </conditional> + + <param name="template_fp" type="data" format="fasta" + label="Fasta file for template alignment" help="(-t/--template_fp)" + optional="True"/> + + <param name="min_length" type="integer" label="Minimum sequence + length to include in alignment" help="By default, 75% of the median + input sequence length (-e/--min_length)" optional="True"/> + + <param name="min_percent_id" type="float" value="0.75" label="Minimum + percent sequence identity to closest blast hit to include sequence + in alignment" help="(-p/--min_percent_id)"/> + </inputs> + + <outputs> + <data name="aligned_sequences" format="fasta" + from_work_dir="align_seqs/*_aligned.fasta" + label="${tool.name} on ${on_string}: Aligned sequences" /> + + <data name="failures_sequences" format="fasta" + from_work_dir="align_seqs/*_failures.fasta" + label="${tool.name} on ${on_string}: Failure sequences"> + <filter> alignment['alignment_method'] == 'pynast'</filter> + </data> + + <data name="log" format="txt" from_work_dir="align_seqs/*_log.txt" + label="${tool.name} on ${on_string}: Log"/> + </outputs> + + <tests> + <test> + <param name="input_fasta_fp" value=""/> + <param name="alignment_method" value="pynast"/> + <param name="pairwise_alignment_method" value="muscle"/> + <param name="min_percent_id" value="0.75" /> + <output name="aligned_sequences" + value="align_seqs_aligned_sequences.fasta"/> + <output name="failures_sequences" + value="align_seqs_failures_sequences.fasta"/> + <output name="log" value="align_seqs_log.txt"/> + </test> + </tests> + + <help><![CDATA[ + +**What it does?** + +This tool aligns the sequences in a FASTA file to each other or to a template sequence alignment, depending on the method chosen. + +More information about this tool is available on +`QIIME documentation <http://qiime.org/scripts/align_seqs.html>`_. + ]]> + </help> + + <citations> + <expand macro="citations" /> + <citation type="doi">10.1093/bioinformatics/btp636</citation> + <citation type="doi">10.1093/nar/gkh340</citation> + <citation type="doi">10.1093/bioinformatics/btp157</citation> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/alpha_diversity.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,112 @@ +<tool id="qiime_alpha_diversity" name="Calculate alpha diversity" version="1.9.1"> + <description>on each sample in an otu table</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <version_command><![CDATA[ + alpha_diversity.py --version + ]]> + </version_command> + + <command> +<![CDATA[ + #set $chosen_metrics = str( $metrics ).split( "," ) + + alpha_diversity.py + -i $input_table + -o $alpha_diversity + + -m $metrics + + #if "PD_whole_tree" in $chosen_metrics: + -t $tree_file + #end if + +]]> + </command> + + <inputs> + <param name="input_table" type="data" format="tabular,txt,tsv,biom" + label="OTU table" help="(-i/--input_path)" + multiple="True"/> + + <param name="metrics" type="select" multiple="True" label="Alpha-diversity + metric(s) to use" help="(-m/--metrics)" + display="checkboxes"> + <option value="ace">ACE (Abundance-based Coverage Estimator) metric</option> + <option value="berger_parker_d">Berger-Parker dominance</option> + <option value="brillouin_d">Brillouin index of alpha diversity</option> + <option value="chao1" selected="true">chao1 richness estimator</option> + <option value="chao1_ci">chao1 confidence interval</option> + <option value="dominance">Dominance</option> + <option value="doubles">Number of double occurrences (doubletons)</option> + <option value="enspie">ENS_pie alpha diversity measure</option> + <option value="equitability">Equitability</option> + <option value="esty_ci">Esty’s CI</option> + <option value="fisher_alpha">Fisher’s alpha</option> + <option value="gini_index">Gini index</option> + <option value="goods_coverage">Good’s coverage of counts</option> + <option value="heip_e">Heip’s evenness measure</option> + <option value="kempton_taylor_q">Kempton-Taylor Q index of alpha diversity</option> + <option value="margalef">Margalef’s richness index</option> + <option value="mcintosh_d">McIntosh dominance index D</option> + <option value="mcintosh_e">McIntosh’s evenness measure E</option> + <option value="menhinick">Menhinick’s richness index</option> + <option value="michaelis_menten_fit">Michaelis-Menten fit to rarefaction curve of observed OTUs</option> + <option value="observed_otus">Number of distinct OTUs</option> + <option value="observed_species" selected="true">Number of distinct species</option> + <option value="osd">Observed OTUs, singles, and doubles</option> + <option value="simpson_reciprocal">simpson_reciprocal</option> + <option value="robbins">Robbins’ estimator for the probability of unobserved outcomes</option> + <option value="shannon">Shannon entropy of counts</option> + <option value="simpson">Simpson’s index</option> + <option value="simpson_e">Simpson’s evenness measure E</option> + <option value="singles">Number of single occurrencesles</option> + <option value="strong"> Strong’s dominance index</option> + <option value="PD_whole_tree">Phylogenetic diversity</option> + </param> + + <param name="tree_file" type="data" label="Newick tree (optional, except + for PD_whole_tree metric" help="(-t/--tree_path)" optional="True"/> + </inputs> + + <outputs> + <data name="alpha_diversity" format="tabular" + from_work_dir="alpha_diversity/alpha_div.txt" + label="${tool.name} on ${on_string}: Alpha diversity" /> + </outputs> + + <tests> + <test> + <param name="input_table" value="pick_open_reference_otus_otu_table.txt"/> + <param name="metrics" value="chao1,observed_species" /> + <output name="alpha_diversity" value="" /> + </test> + </tests> + + <help><![CDATA[ + +**What it does?** + +This tool calculates alpha diversity, or within-sample diversity, using an +OTU table. + +The resulting file(s) is a tab-delimited text file, where the columns correspond +to alpha diversity metrics and the rows correspond to samples and their calculated +diversity measurements. When a folder is given as input (-i), the script processes +every otu table file in the given folder, and creates a corresponding file in the +output directory. + +More information about this tool is available on +`QIIME documentation <http://qiime.org/scripts/alpha_diversity.html>`_. + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/alpha_rarefaction.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,117 @@ +<tool id="qiime_alpha_rarefaction" name="Perform alpha rarefaction" version="1.9.1"> + + <description></description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <version_command><![CDATA[ + alpha_rarefaction.py --version + ]]> + </version_command> + + <command> +<![CDATA[ + alpha_rarefaction.py + -i $otu_table_fp + -m $mapping_fp + -o alpha_rarefaction + + #if $parameter_fp + -p $parameter_fp + #end if + + -n $num_steps + $parallel + -O "\${GALAXY_SLOTS:-4}" + + #if $tree_fp + -t $tree_fp + #end if + + --min_rare_depth $min_rare_depth + + #if $max_rare_depth + -e $max_rare_depth + #end if + + && + mkdir -p $alpha_rarefaction_plots.files_path + && + cp -r alpha_rarefaction/alpha_rarefaction_plots/rarefaction_plots.html $alpha_rarefaction_plots.files_path + && + cp -r alpha_rarefaction/alpha_rarefaction_plots/average_plots $alpha_rarefaction_plots.files_path + && + mv $alpha_rarefaction_plots.files_path/rarefaction_plots.html $alpha_rarefaction_plots +]]> + </command> + + <inputs> + <param name="otu_table_fp" type="data" format="tabular,txt,tsv,biom" + label="OTU table" help="(-i/--otu_table_fp)" /> + + <param name="mapping_fp" type="data" format="tabular,txt,tsv" + label="Mapping file" help="(-m/--mapping_fp)" /> + + <param name="parameter_fp" type="data" format="txt" + label="Parameter file" help="It specifies changes to the default + behavior (-p/--parameter_fp)" optional="true"/> + + <param name="num_steps" type="integer" value="10" label="Number of steps + (or rarefied OTU table sizes) to make between min and max counts" + help="(-n/--num_steps)"/> + + <param name="parallel" type="boolean" label="Run in parallel where + available?" truevalue="--parallel" falsevalue="" checked="True" + help="(-a, --parallel)" /> + + <param name="tree_fp" type="data" format="tabular,txt,tsv" + label="Tree file (optional)" help="Required for phylogenetic measures + (-t/--tree_fp)" optional="True"/> + + <param name="min_rare_depth" type="integer" value="10" label="Lower limit + of rarefaction depths" help="(--min_rare_depth)"/> + + <param name="max_rare_depth" type="integer" label="Upper limit + of rarefaction depths" help="By default, median sequence/sample count + is used (-e/--max_rare_depth)" optional="True"/> + </inputs> + + <outputs> + <data name="alpha_rarefaction_plots" format="html" + label="${tool.name} on ${on_string}: Alpha rarefaction plots"/> + + <data format="txt" name="alpha_diversity"> + <discover_datasets pattern="__designation__" ext="txt" + directory="alpha_rarefaction/alpha_div_collated" visible="true" /> + </data> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ + +**What it does** + +This script performs alpha rarefaction in several steps: + + - Generation of rarefied OTU tables + - Computation of alpha diversity metrics for each rarefied OTU table + - Concatenation of alpha diversity results + - Generation of alpha rarefaction plots + +More information about this tool is available on +`QIIME documentation <http://qiime.org/scripts/alpha_rarefaction.html>`_. + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/assign_taxonomy.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,304 @@ +<tool id="qiime_assign_taxonomy" name="assign taxonomy" version="1.9.1galaxy1"> + + <description>Assign taxonomy to each sequence</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <command> +<![CDATA[ + assign_taxonomy.py -i $input_fasta_fp + + #if str($id_to_taxonomy_fp) != 'None': + -t $id_to_taxonomy_fp + #end if + + #if str($reference_seqs_fp) != 'None': + -r $reference_seqs_fp + #end if + + #if str($methodcond.assignment_method) = 'None': + -m uclust + #end if + + #if str($methodcond.assignment_method) != 'None': + -m $methodcond.assignment_method + #end if + + #if $methodcond.assignment_method == "rtax": + + #if $methodcond.single_ok: + --single_ok + #end if + + #if $methodcond.no_single_ok_generic: + --no_single_ok_generic + #end if + + #if str($methodcond.read_id_regex): + --read_id_regex=$methodcond.read_id_regex + #end if + + #if str($methodcond.amplicon_id_regex): + --amplicon_id_regex=$methodcond.amplicon_id_regex + #end if + + #if str($methodcond.header_id_regex): + --header_id_regex=$methodcond.header_id_regex + #end if + #end if + + #if $methodcond.assignment_method == "sortmerna": + + #if str($methodcond.sortmerna_db): + --sortmerna_db=$methodcond.sortmerna_db + #end if + + #if $methodcond.sortmerna_e_value: + --sortmerna_e_value=$methodcond.sortmerna_e_value + #end if + + #if $methodcond.sortmerna_coverage: + --sortmerna_coverage=$methodcond.sortmerna_coverage + #end if + + #if $methodcond.sortmerna_best_N_alignments: + --sortmerna_best_N_alignments=$methodcond.sortmerna_best_N_alignments + #end if + + #if str($methodcond.sortmerna_threads): + --sortmerna_threads=$methodcond.sortmerna_threads + #end if + + #if $methodcond.min_consensus_fraction: + --min_consensus_fraction=$methodcond.min_consensus_fraction + #end if + + #if $methodcond.similarity: + --similarity=$methodcond.similarity + #end if + #end if + + #if $methodcond.assignment_method == "blast": + + #if str($methodcond.blast_db) != 'None': + -b \$BLAST_DB_NAME + #end if + + #if $methodcond.blast_e_value: + -e $methodcond.blast_e_value + #end if + #end if + + #if $methodcond.assignment_method == "rdp": + + #if $methodcond.confidence: + -c $methodcond.confidence + #end if + + #if $methodcond.rdp_max_memory: + --rdp_max_memory=$methodcond.rdp_max_memory + #end if + #end if + + #if $methodcond.assignment_method == "mothur": + + #if $methodcond.confidence: + -c $methodcond.confidence + #end if + #end if + + #if $methodcond.assignment_method == "uclust": + + #if $methodcond.min_consensus_fraction: + --min_consensus_fraction=$methodcond.min_consensus_fraction + #end if + + #if $methodcond.similarity: + --similarity=$methodcond.similarity + #end if + + #if $methodcond.uclust_max_accepts: + --uclust_max_accepts=$methodcond.uclust_max_accepts + #end if + #end if + -o assign_taxonomy_output +]]> + </command> + + <inputs> + <param label="-i/--input_fasta_fp: path to the input fasta file" + name="input_fasta_fp" optional="False" type="data"/> + <param default="/home12/caparmor/bioinfo/softs/sources/Qiime/data/gg_13_8_otus/taxonomy/99_otu_taxonomy.txt" + label="-t/--id_to_taxonomy_fp: Path to tab-delimited file mapping + sequences to assigned taxonomy. Each assigned taxonomy is provided as + a semicolon-separated list. For assignment with rdp, each assigned + taxonomy must be exactly 6 levels deep. [default: /home12/caparmor/bioinfo/softs/sources/Qiime/data/gg_13_8_otus/taxonomy/99_otu_taxonomy.txt]" + name="id_to_taxonomy_fp" optional="True" type="data"/> + <param default="/home12/caparmor/bioinfo/softs/sources/Qiime/data/gg_13_8_otus/rep_set/99_otus.fasta" + label="-r/--reference_seqs_fp: Path to reference sequences. For + assignment with blast, these are used to generate a blast database. + For assignment with rdp, they are used as training sequences for the + classifier. [default: /home12/caparmor/bioinfo/softs/sources/Qiime/data/gg_13_8_otus/rep_set/99_otus.fasta]" + name="reference_seqs_fp" optional="True" type="data"/> + + <conditional name="methodcond"> + <param label="-m/--assignment_method: Taxon assignment method, must be + one of rdp, blast, rtax, mothur, uclust, sortmerna [default: uclust]" + name="assignment_method" optional="False" type="select"> + <option selected="True" value="uclust">uclust</option> + <option value="rdp">rdp</option> + <option value="blast">blast</option> + <option value="rtax">rtax</option> + <option value="mothur">mothur</option> + <option value="sortmerna">sortmerna</option> + </param> + <when value="rtax"> + <param label="--single_ok: When classifying paired ends, allow + fallback to single-ended classification when the mate pair is + lacking (used for RTAX only). [default: False]" name="single_ok" + selected="False" type="boolean"/> + <param label="--no_single_ok_generic: When classifying paired ends, + do not allow fallback to single-ended classification when the + mate pair is overly generic (used for RTAX only). [default: False]" + name="no_single_ok_generic" selected="False" type="boolean"/> + <param default="\S+\s+(\S+)" label="--read_id_regex: Used to parse + the result of OTU clustering, to get the read_1_id for each + clusterID. The clusterID itself is assumed to be the first + field, and is not captured by the regex. (used for RTAX only). + [default: \S+\s+(\S+)]" name="read_id_regex" optional="True" + type="text"/> + <param default="(\S+)\s+(\S+?)\/" label="--amplicon_id_regex: Used + to parse the result of split_libraries, to get the ampliconID + for each read_1_id. Two groups capture read_1_id and ampliconID, + respectively. (used for RTAX only). [default: (\S+)\s+(\S+?)\/]" + name="amplicon_id_regex" optional="True" type="text"/> + <param default="\S+\s+(\S+?)\/" label="--header_id_regex: Used to + parse the result of split_libraries, to get the portion of the + header that RTAX uses to match mate pairs. The default uses + the amplicon ID, not including /1 or /3, as the primary key + for the query sequences. Typically this regex will be the + same as amplicon_id_regex, except that only the second group + is captured. (used for RTAX only). [default: \S+\s+(\S+?)\/]" + name="header_id_regex" optional="True" type="text"/> + </when> + <when value="sortmerna"> + <param label="--sortmerna_db: Pre-existing database to search + against when using sortmerna [default: None]" name="sortmerna_db" + optional="True" type="text"/> + <param default="1.0" label="--sortmerna_e_value: Maximum E-value + when clustering [default = 1.0]" name="sortmerna_e_value" + optional="True" type="float"/> + <param default="0.9" label="--sortmerna_coverage: Mininum percent + query coverage (of an alignment) to consider a hit, expressed + as a fraction between 0 and 1 [default: 0.9]" + name="sortmerna_coverage" optional="True" type="float"/> + <param default="5" label="--sortmerna_best_N_alignments: This option + specifies how many best alignments per read will be written + [default: 5]" name="sortmerna_best_N_alignments" optional="True" + type="integer"/> + <param default="1" label="--sortmerna_threads: Specify number of + threads to be used for sortmerna mapper which utilizes multithreading. + [default: 1]" name="sortmerna_threads" optional="True" + type="text"/> + <param default="0.51" label="--min_consensus_fraction: Minimum + fraction of database hits that must have a specific taxonomic + assignment to assign that taxonomy to a query, only used for + sortmerna and uclust methods [default: 0.51]" + name="min_consensus_fraction" optional="True" type="float"/> + <param default="0.9" label="--similarity: Minimum percent similarity + (expressed as a fraction between 0 and 1) to consider a database + match a hit, only used for sortmerna and uclust methods + [default: 0.9]" name="similarity" optional="True" type="float"/> + </when> + <when value="blast"> + <param label="-b/--blast_db: Database to blast against. Must provide + either --blast_db or --reference_seqs_db for assignment with blast + [default: None]" name="blast_db" optional="True" type="data"/> + <param default="0.001" label="-e/--blast_e_value: Maximum e-value + to record an assignment, only used for blast method [default: + 0.001]" name="blast_e_value" optional="True" type="float"/> + </when> + <when value="rdp"> + <param default="0.5" label="-c/--confidence: Minimum confidence to + record an assignment, only used for rdp and mothur methods + [default: 0.5]" name="confidence" optional="True" type="float"/> + <param default="4000" label="--rdp_max_memory: Maximum memory + allocation, in MB, for Java virtual machine when using the + rdp method. Increase for large training sets [default: 4000]" + name="rdp_max_memory" optional="True" type="integer"/> + </when> + <when value="mothur"> + <param default="0.5" label="-c/--confidence: Minimum confidence to + record an assignment, only used for rdp and mothur methods + [default: 0.5]" name="confidence" optional="True" type="float"/> + </when> + <when value="uclust"> + <param default="0.51" label="--min_consensus_fraction: Minimum + fraction of database hits that must have a specific taxonomic + assignment to assign that taxonomy to a query, only used for + sortmerna and uclust methods [default: 0.51]" name="min_consensus_fraction" + optional="True" type="float"/> + <param default="0.9" label="--similarity: Minimum percent similarity + (expressed as a fraction between 0 and 1) to consider a database + match a hit, only used for sortmerna and uclust methods [default: + 0.9]" name="similarity" optional="True" type="float"/> + <param default="3" label="--uclust_max_accepts: Number of database + hits to consider when making an assignment, only used for uclust + method [default: 3]" name="uclust_max_accepts" optional="True" + type="integer"/> + </when> + </conditional> + </inputs> + + <outputs> + <data format="txt" from_work_dir="assign_taxonomy_output/*.log" + label="tax_assignements.log" name="tax_assignements.log"/> + <data format="txt" from_work_dir="assign_taxonomy_output/*.txt" + label="tax_assignements.txt" name="tax_assignements.txt"/> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ +**What it does** + +Contains code for assigning taxonomy, using several techniques. + +Given a set of sequences, %prog attempts to assign the taxonomy of each sequence. +Currently the methods implemented are assignment with BLAST, the RDP classifier, +RTAX, mothur, and uclust. The output of this step is an observation metadata +mapping file of input sequence identifiers (1st column of output file) to taxonomy +(2nd column) and quality score (3rd column). There may be method-specific information +in subsequent columns. + +Reference data sets and id-to-taxonomy maps for 16S rRNA sequences can be found in +the Greengenes reference OTU builds. To get the latest build of the Greengenes OTUs +(and other marker gene OTU collections), follow the "Resources" link from http://qiime.org. +After downloading and unzipping you can use the following files as -r and -t, where +<otus_dir> is the name of the new directory after unzipping the reference OTUs tgz + file. + +-r <otus_dir>/rep_set/97_otus.fasta +-t <otus_dir></otus_dir>/taxonomy/97_otu_taxonomy.txt + +The consensus taxonomy assignment implemented here is the most detailed lineage +description shared by 90% or more of the sequences within the OTU (this level of +agreement can be adjusted by the user). The full lineage information for each +sequence is one of the output files of the analysis. In addition, a conflict file +records cases in which a phylum-level taxonomy assignment disagreement exists +within an OTU (such instances are rare and can reflect sequence misclassification +within the greengenes database). + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/beta_diversity.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,120 @@ +<tool id="qiime_beta_diversity" name="Calculate beta diversity" version="1.9.1"> + + <description>(pairwise sample dissimilarity)</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <version_command><![CDATA[ + beta_diversity.py --version + ]]> + </version_command> + + <stdio> + <regex match="VisibleDeprecationWarning" + source="both" + level="warning" + description="VisibleDeprecationWarning on numpy for `rank`" /> + </stdio> + + <command> +<![CDATA[ + #set $chosen_metrics = str( $metrics ).split( "," ) + + beta_diversity.py + -i $otu_table_fp + + #if $rows + -r $rows + #end if + + -o beta_diversity + -m $metrics + + #if "unifrac_g" in $chosen_metrics or "unifrac_g_full_tree" in $chosen_metrics or "unweighted_unifrac" in $chosen_metrics or "unweighted_unifrac_full_tree" in $chosen_metrics or "weighted_normalized_unifrac" in $chosen_metrics or "weighted_unifrac" in $chosen_metrics + -t $tree_file + #end if +]]> + </command> + + <inputs> + <param name="otu_table_fp" type="data" format="tabular,txt,tsv" + label="OTU table" help="(-i/--otu_table_fp)" /> + + <param name="rows" type="text" value="" + label="Rows on which distance matrix is computed (optional)" help="A + comma-separated list of sample names is expected (-i/--otu_table_fp)" + optional="true"/> + + <param name="metrics" type="select" multiple="True" label="Beta-diversity + metric(s) to use" help="(-m/--metrics)" + display="checkboxes"> + <option value="abund_jaccard">Abundance weighted Jaccard distance</option> + <option value="binary_chisq">Binary chi-square distance</option> + <option value="binary_chord">Binary chord distance</option> + <option value="binary_euclidean">Binary euclidean distance</option> + <option value="binary_hamming">Binary Hamming distance (binary Manhattan distance)</option> + <option value="binary_jaccard">Binary Jaccard distance (binary Soergel distance)</option> + <option value="binary_lennon">Binary Lennon distance</option> + <option value="binary_ochiai">Binary Ochiai distance</option> + <option value="binary_pearson">Binary Pearson distance</option> + <option value="binary_sorensen_dice">Binary Sörensen-Dice distance (binary Bray-Curtis distance or binary Whittaker distance)</option> + <option value="bray_curtis">Bray-Curtis distance (normalized Manhattan distance)</option> + <option value="canberra">Canberra distance</option> + <option value="chisq">Chi-square distance</option> + <option value="chord">Chord distance</option> + <option value="euclidean">Euclidean distance</option> + <option value="gower">Gower distance</option> + <option value="hellinger">Hellinger distance</option> + <option value="kulczynski">Kulczynski distance</option> + <option value="manhattan">Manhattan distance</option> + <option value="morisita_horn">Morisita-Horn distance</option> + <option value="pearson">Pearson distance</option> + <option value="soergel">Soergel distance</option> + <option value="spearman_approx">Spearman rank distance</option> + <option value="specprof">Species profile distance</option> + <option value="unifrac_g">G UniFrac metric (phylogenetic)</option> + <option value="unifrac_g_full_tree">G UniFrac full tree metric (phylogenetic)</option> + <option value="unweighted_unifrac" selected="true">Unweighted UniFrac metric (phylogenetic)</option> + <option value="unweighted_unifrac_full_tree">Unweighted UniFrac full tree metric (phylogenetic)</option> + <option value="weighted_normalized_unifrac">Normalized weighted UniFrac metric (phylogenetic)</option> + <option value="weighted_unifrac" selected="true">Weighted UniFrac metric (phylogenetic)</option> + </param> + + <param name="tree_file" type="data" label="Newick tree (optional, except + for PD_whole_tree metric" help="(-t/--tree_path)" optional="True"/> + </inputs> + + <outputs> + <data format="txt" name="beta_diversity"> + <discover_datasets pattern="__designation__" ext="txt" + directory="beta_diversity" visible="true" /> + </data> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ + +**What it does** + +This script generate distance matrices containing a dissimilarity value for each +pairwise comparison. + +A number of metrics are currently supported, including unweighted and weighted UniFrac (pass the -s option to see available metrics). In general, because unifrac uses phylogenetic information, one of the unifrac metrics is recommended, as results can be vastly more useful (Hamady & Knight, 2009). Quantitative measures (e.g. weighted unifrac) are ideally suited to revealing community differences that are due to changes in relative taxon abundance (e.g., when a particular set of taxa flourish because a limiting nutrient source becomes abundant). Qualitative measures (e.g. unweighted unifrac) are most informative when communities differ primarily by what can live in them (e.g., at high temperatures), in part because abundance information can obscure significant patterns of variation in which taxa are present (Lozupone et al., 2007). Most qualitative measures are referred to here e.g. “binary_jaccard”. Typically both weighted and unweighted unifrac are used. + +More information about this tool is available on +`QIIME documentation <http://qiime.org/scripts/beta_diversity.html>`_. + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/beta_diversity_through_plots.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,132 @@ +<tool id="qiime_beta_diversity_through_plots" name="Compute beta diversity distance + matrices" version="1.9.1"> + + <description>and generate PCoA plots</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <version_command><![CDATA[ + beta_diversity_through_plots.py --version + ]]> + </version_command> + + <stdio> + <regex match="VisibleDeprecationWarning" + source="both" + level="warning" + description="VisibleDeprecationWarning on numpy for `rank`" /> + </stdio> + + <command> +<![CDATA[ + beta_diversity_through_plots.py + -i $otu_table_fp + -m $mapping_fp + -o beta_diversity_through_plots + + #if $tree_fp + -t $tree_fp + #end if + + #if $parameter_fp + -p $parameter_fp + #end if + + $parallel + -O "\${GALAXY_SLOTS:-4}" + + #if $seqs_per_sample + -e $seqs_per_sample + #end if + + $suppress_emperor_plots + + && + + python $__tool_directory__/beta_diversity_through_plots_html_generation.py + --data_directory beta_diversity_through_plots + --html_file $beta_diversity_pcoa + --html_dir $beta_diversity_pcoa.files_path + + ]]> + </command> + + <inputs> + <param name="otu_table_fp" type="data" format="tabular,txt,tsv,biom" + label="OTU table" help="(-i/--otu_table_fp)" + multiple="True"/> + + <param name="mapping_fp" type="data" format="tabular,txt,tsv" + label="Mapping file" help="(-m/--mapping_fp)" + multiple="True"/> + + <param name="tree_fp" type="data" format="txt" + label="Tree file (Optional)" help="(-t/--tree_fp)" + optional="True"/> + + <param name="parameter_fp" type="data" format="txt" + label="Parameter file (Optional)" help="It specifies changes to the default + behavior, e.g. beta diversity metrics (-p/--parameter_fp)" + optional="true"/> + + <param name="color_by_all_fields" type="text" label="Colored mapping + fields" optional="True" help="Can be included only fields with + greater than one value and fewer values than the number of samples + (--color_by_all_fields)" /> + + <param name="parallel" type="boolean" label="Run in parallel where + available?" truevalue="--parallel" falsevalue="" checked="False" + help="(-a, --parallel)" /> + + <param name="seqs_per_sample" type="integer" label="Depth of coverage for + even sampling (Optional)" help="(-e/--seqs_per_sample)" optional="True"/> + + <param name="suppress_emperor_plots" type="boolean" label="Generate + emperor plots?" truevalue="" falsevalue="--suppress_emperor_plots" checked="True" + help="(--suppress_emperor_plots)" /> + + </inputs> + + <outputs> + <data format="txt" name="beta_diversity_matrix"> + <discover_datasets pattern="(?P<designation>.+)_dm\.txt" ext="txt" + directory="beta_diversity_through_plots" visible="true" /> + </data> + + <data format="txt" name="beta_diversity_pc"> + <discover_datasets pattern="(?P<designation>.+)_pc\.txt" ext="txt" + directory="beta_diversity_through_plots" visible="true" /> + </data> + + <data format="html" name="beta_diversity_pcoa" + label="${tool.name} on ${on_string}: PCoA"/> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ +**What it does** + +This tool computes beta diversity distance matrices and generates PCoA plots in +several steps + + - Performs beta diversity analysis + - Performs principal coordinate analysis + - Generates 3D PCoA Plots + +More information about this tool is available on +`QIIME documentation <http://qiime.org/scripts/beta_diversity_through_plots.html>`_. + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/beta_diversity_through_plots_html_generation.py Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,56 @@ +#!/usr/bin/env python +# -*- coding: utf-8 -*- + +import sys +import os +import argparse +import re + + +def generate_index_html(dir_list, args): + with open('index.html','w') as index_html_file: + index_html_file.write('<html>\n') + index_html_file.write('\t<head><title>PCoA beta diversity results</title></head>\n') + index_html_file.write('\t<body>\n') + index_html_file.write('\t\t<a href="http://www.qiime.org" target="_blank"><img src="http://qiime.org/_static/wordpressheader.png" alt="www.qiime.org""/></a>\n') + index_html_file.write('\t\t<p>\n') + index_html_file.write('\t\t\tBeta diversity metrics\n') + index_html_file.write('\t\t\t<ul>\n') + + for directory in dir_list: + regexp_result = re.match('([a-zA-Z]*)_emperor_pcoa_plot',directory) + metric = regexp_result.group(1) + index_html_file.write('\t\t\t\t<li>' + metric + ': ') + index_html_file.write('<a href="' + directory + + '/index.html">PCoA results</a></td>\n') + index_html_file.write('\t\t\t\t</li>\n') + + index_html_file.write('\t\t\t</ul>\n') + index_html_file.write('\t\t</p>\n') + index_html_file.write('\t</body>\n') + index_html_file.write('</html>\n') + +def build_html(args): + os.mkdir(args.html_dir) + + dir_list = [ name for name in os.listdir(args.data_directory) if + os.path.isdir(os.path.join(args.data_directory, name)) ] + + generate_index_html(dir_list, args) + + os.system('cp -r index.html ' + args.html_dir) + + for directory in dir_list: + os.system('cp -r ' + args.data_directory + '/' + directory + ' ' + + args.html_dir) + + os.system('mv ' + args.html_dir + '/index.html ' + args.html_file) + +if __name__ == '__main__': + parser = argparse.ArgumentParser() + parser.add_argument('--data_directory', required=True) + parser.add_argument('--html_file', required=True) + parser.add_argument('--html_dir', required=True) + args = parser.parse_args() + + build_html(args)
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/beta_diversity_through_plots_wrapper.py Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,124 @@ +#!/usr/bin/env python + +# create a html output for beta_diverity_through_plots.py +# to replace beta_diversity_through_plot_html.py in qiime 1.8 +# Leroi Laura laura.leroi@ifremer.fr + +import optparse +import os +import sys +import subprocess + +# Create html template +def html_maker(metrics): + + tag="" + plots = metrics.split(',') + for i in range(0, len(plots)): + tag = tag+"""\t\t<option value='%s_emperor_pcoa_plot/index.html'>%s</option>\n""" % (plots[i], plots[i]) + template_html= """ +<!doctype html> +<html lang="en"> +<head> + <meta charset="utf-8"> + <script type="text/javascript" src="/galaxy/static/scripts/libs/jquery/jquery.js"></script> +</head> +<body> + <label for="outputs">Select a beta-diversity metric :</label> + <select id="outputs" name="outputs"> +%s + </select> + <br/> + <br/> + <iframe id="plot" name="plot" style="position:absolute; width: 90%%; height: 90%%;" src="" frameborder="0"></iframe> + <script> + $(document).ready(function(){ + var str = ""; + $( "#outputs option:selected" ).each(function() { + str += $( this ).val() + " "; + $("#plot").attr("src", str); + }); + + }); + $( "#outputs" ).change(function () { + var str = ""; + $( "#outputs option:selected" ).each(function() { + str += $( this ).val() + " "; + $("#plot").attr("src", str); + }); + }).change(); +</script> +</body> +</html> """ % (tag) + return template_html + + +def html_matrix_maker(metrics): + + tag="" + plots = metrics.split(',') + for i in range(0, len(plots)): + tag = tag+"""\t\t<a href='%s_dm.txt'>%s_dm</a><br/>\n""" % (plots[i], plots[i]) + + template_html= """ +<!doctype html> +<html lang="en"> +<body> + <label>Metrics matrix : </label><br/> +%s +</body> +</html> """ % (tag) + return template_html + + +def main(): + + #On importe l environnemnent envqiime + pipe = subprocess.Popen(". /home12/caparmor/bioinfo/GALAXY_DEV/ourenv/envqiime; python -c 'import os; print \"newenv = %r\" % os.environ'", stdout=subprocess.PIPE, shell=True) + exec(pipe.communicate()[0]) + os.environ.update(newenv) + + #New parser + parser = optparse.OptionParser() + #beta diversity options + parser.add_option('--metrics',dest='metrics') + parser.add_option('--biom',dest='biom') + parser.add_option('--mapping',dest='mapping') + parser.add_option('--tree',dest='tree') + parser.add_option('--depth',dest='depth') + parser.add_option('--masterhtml',dest='masterhtml') + parser.add_option('--outputfolder',dest='outputfolder') + parser.add_option('--matrixhtml',dest='matrixhtml') + + (options,args) = parser.parse_args() + + #New hmtl template + master = open('./master.html', 'w') + master.write(html_maker(options.metrics)) + master.close() + #On lance le fichier avec les options precisees pour l'affichage html + os.system("mv master.html %s" % options.masterhtml) + + #parameter file, which specifies changes to the default behavior + parameters = open('./parameters.txt','w') + parameters.write("beta_diversity:metrics %s" % options.metrics) + parameters.close() + + if options.depth: + if options.tree : + os.system("beta_diversity_through_plots.py -i %s -m %s -t %s -e %s -o %s -p ./parameters.txt >& file_log.txt" % (options.biom, options.mapping, options.tree, options.depth, options.outputfolder)) + else : + os.system("beta_diversity_through_plots.py -i %s -m %s -e %s -o %s -p ./parameters.txt >& file_log.txt" % (options.biom, options.mapping, options.depth, options.outputfolder)) + else: + if options.tree : + os.system("beta_diversity_through_plots.py -i %s -m %s -t %s -o %s -p ./parameters.txt >& file_log.txt" % (options.biom, options.mapping, options.tree, options.outputfolder)) + else : + os.system("beta_diversity_through_plots.py -i %s -m %s -o %s -p ./parameters.txt >& file_log.txt" % (options.biom, options.mapping, options.outputfolder)) + + os.system("mkdir %s/txt" % (options.outputfolder)) + os.system("mv %s/*_dm.txt %s/txt" %(options.outputfolder,options.outputfolder)) + os.system("compress_path.py -i %s/txt -o %s" % (options.outputfolder,options.matrixhtml)) + +if __name__=="__main__": + main() +
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/compare_categories.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,177 @@ +<tool id="qiime_compare_categories" name="compare categories" version="1.9.1galaxy1"> + + <description>Analyzes statistical significance of sample groupings using + distance matrices</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <command> +<![CDATA[ + compare_categories.py + --method=$method + -i $input_dm + -m $mapping_file + -c $categories + -o compare_categories_output + #if $num_permutations: + -n $num_permutations + #end if +]]> + </command> + + <inputs> + <param label="--method: the statistical method to use. Valid options: + adonis, anosim, bioenv, morans_i, mrpp, permanova, permdisp, + dbrda" name="method" optional="False" type="select"> + <option value="adonis">adonis</option> + <option value="anosim">anosim</option> + <option value="bioenv">bioenv</option> + <option value="morans_i">morans_i</option> + <option value="mrpp">mrpp</option> + <option value="permanova">permanova</option> + <option value="permdisp">permdisp</option> + <option value="dbrda">dbrda</option> + </param> + <param label="-i/--input_dm: the input distance matrix. WARNING: Only + symmetric, hollow distance matrices may be used as input. Asymmetric + distance matrices, such as those obtained by the UniFrac Gain metric + (i.e. beta_diversity.py -m unifrac_g), should not be used as input" + name="input_dm" optional="False" type="data"/> + <param label="-m/--mapping_file: the metadata mapping file" + name="mapping_file" optional="False" type="data"/> + <param label="-c/--categories: a comma-delimited list of categories from + the mapping file. Note: all methods except for BIO-ENV accept just a + single category. If multiple categories are provided, only the first + will be used" name="categories" optional="False" type="text"/> + <param default="999" label="-n/--num_permutations: the number of permutations + to use when calculating statistical significance. Only applies to + adonis, ANOSIM, MRPP, PERMANOVA, PERMDISP, and db-RDA. Must be greater + than or equal to zero [default: 999]" name="num_permutations" + optional="True" type="integer"/> + </inputs> + + <outputs> + <data format="txt" from_work_dir="compare_categories_output/*.txt" + name="output_dir" label="Compare_categories.txt"/> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ +**What it does** + +This script allows for the analysis of the strength and statistical +significance of sample groupings using a distance matrix as the primary input. +Several statistical methods are available: adonis, ANOSIM, BIO-ENV, Moran's I, +MRPP, PERMANOVA, PERMDISP, and db-RDA. + +Note: R's vegan and ape packages are used to compute many of these methods, and +for the ones that are not, their implementations are based on the +implementations found in those packages. It is recommended to read through the +detailed descriptions provided by the authors (they are not reproduced here) +and to refer to the primary literature for complete details, including the +methods' assumptions. To view the documentation of a method in R, prepend a +question mark before the method name. For example: + +?vegan::adonis + +The following are brief descriptions of the available methods: + +adonis - Partitions a distance matrix among sources of variation in order to +describe the strength and significance that a categorical or continuous +variable has in determining variation of distances. This is a nonparametric +method and is nearly equivalent to db-RDA (see below) except when distance +matrices constructed with semi-metric or non-metric dissimilarities are +provided, which may result in negative eigenvalues. adonis is very similar to +PERMANOVA, though it is more robust in that it can accept either categorical or +continuous variables in the metadata mapping file, while PERMANOVA can only +accept categorical variables. See vegan::adonis for more details. + +ANOSIM - Tests whether two or more groups of samples are significantly +different based on a categorical variable found in the metadata mapping file. +You can specify a category in the metadata mapping file to separate +samples into groups and then test whether there are significant differences +between those groups. For example, you might test whether 'Control' samples are +significantly different from 'Fast' samples. Since ANOSIM is nonparametric, +significance is determined through permutations. See vegan::anosim for more +details. + +BIO-ENV - Finds subsets of variables whose Euclidean distances (after scaling +the variables) are maximally rank-correlated with the distance matrix. For +example, the distance matrix might contain UniFrac distances between +communities, and the variables might be numeric environmental variables (e.g., +pH and latitude). Correlation between the community distance matrix and +Euclidean environmental distance matrix is computed using Spearman's rank +correlation coefficient (rho). This method will only accept categories that are +numerical (continuous or discrete). This is currently the only method in the +script that accepts more than one category (via -c). See vegan::bioenv for more +details. This method is also known as BEST (previously called BIO-ENV) in the +PRIMER-E software package. + +Moran's I - This method uses the numerical (e.g. geographical) data supplied to +identify what type of spatial configuration occurs in the samples. For example, +are they dispersed, clustered, or of no distinctly noticeable configuration +when compared to each other? This method will only accept a category that is +numerical. See ape::Moran.I for more details. + +MRPP - This method tests whether two or more groups of samples are +significantly different based on a categorical variable found in the metadata +mapping file. You can specify a category in the metadata mapping file to +separate samples into groups and then test whether there are significant +differences between those groups. For example, you might test whether 'Control' +samples are significantly different from 'Fast' samples. Since MRPP is +nonparametric, significance is determined through permutations. See +vegan::mrpp for more details. + +PERMANOVA - This method is very similar to adonis except that it only accepts a +categorical variable in the metadata mapping file. It uses an ANOVA +experimental design and returns a pseudo-F value and a p-value. Since PERMANOVA +is nonparametric, significance is determined through permutations. + +PERMDISP - This method analyzes the multivariate homogeneity of group +dispersions (variances). In essence, it determines whether the variances of +groups of samples are significantly different. The results of both parametric +and nonparametric significance tests are provided in the output. This method is +generally used as a companion to PERMANOVA. See vegan::betadisper for more +details. + +db-RDA - This method is very similar to adonis and will only differ if certain +non-Euclidean semi- or non-metrics are used to generate the input distance +matrix, and negative eigenvalues are encountered. The only difference then will +be in the p-values, not the R^2 values. As part of the output, an ordination +plot is also generated that shows grouping/clustering of samples based on a +category in the metadata mapping file. This category is used to explain the +variability between samples. Thus, the ordination output of db-RDA is similar +to PCoA except that it is constrained, while PCoA is unconstrained (i.e. with +db-RDA, you must specify which category should be used to explain the +variability in your data). See vegan::capscale for more details. + +For more information and examples pertaining to this script, please refer to +the accompanying tutorial, which can be found at +http://qiime.org/tutorials/category_comparison.html. + + +At least one file will be created in the output directory specified by -o. For +most methods, a single output file containing the results of the test (e.g. the +effect size statistic and p-value) will be created. The format of the output +files will vary between methods as some are generated by native QIIME code, +while others are generated by R's vegan or ape packages. Please refer to the +script description for details on how to access additional information for +these methods, including what information is included in the output files. + +db-RDA is the only exception in that two output files are created: a results +text file and a PDF of the ordination plot. + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/core_diversity_analyses.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,143 @@ +<tool id="qiime_core_diversity" name="Compute core set of diversity analyses" version="1.9.1"> + <description></description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <version_command><![CDATA[ + core_diversity_analyses.py --version + ]]> + </version_command> + + <command> +<![CDATA[ + core_diversity_analyses.py + -i $input_biom_fp + -o core_diversity_analyses + -m $mapping_fp + -e $sampling_depth + + #if $parameter_fp + -p $parameter_fp + #end if + + $parallel + -O "\${GALAXY_SLOTS:-4}" + + #if $phylogenetic.nonphylogenetic_diversity + --nonphylogenetic_diversity + #else + -t $phylogenetic.tree_fp + #end if + + $suppress_taxa_summary + $suppress_beta_diversity + $suppress_alpha_diversity + $suppress_group_significance + + #if $categories + -c $categories + #end if + + && + mkdir -p $html_report.files_path + && + cp -r core_diversity_analyses/* $html_report.files_path + && + mv $html_report.files_path/index.html $html_report +]]> + </command> + + <inputs> + <param name="input_biom_fp" type="data" format="tabular,txt,tsv,biom" + label="OTU table" help="(-i/--input_biom_fp)"/> + + <param name="mapping_fp" type="data" format="tabular,txt,tsv" + label="Mapping file" help="(-m/--mapping_fp)"/> + + <param name="sampling_depth" type="integer" value="" label="Sequencing + depth to use for even sub-sampling and maximum rarefaction depth" + help="To decide this value, review the output of the + ‘biom summarize-table’ (-e/--sampling_depth)"/> + + <param name="parameter_fp" type="data" format="txt" + label="Parameter file" help="It specifies changes to the default + behavior (-p/--parameter_fp)" optional="true"/> + + <param name="parallel" type="boolean" label="Run in parallel where + available?" truevalue="--parallel" falsevalue="" checked="true" + help="(-a, --parallel)" /> + + <conditional name="phylogenetic"> + <param name="nonphylogenetic_diversity" type="boolean" label="Apply + non-phylogenetic alpha (chao1 and observed_otus) and beta (bray_curtis) + diversity calculations?" truevalue="--nonphylogenetic_diversity" + falsevalue="" checked="false" help="This is useful if, for example, + when working with non-amplicon BIOM tables, or if a reliable tree + is not available (--nonphylogenetic_diversity)" /> + <when value="false" > + <param name="tree_fp" type="data" format="txt" + label="Tree file" help="(-t/--tree_fp)"/> + </when> + <when value="true" /> + </conditional> + + <param name="suppress_taxa_summary" type="boolean" label="Suppress + generation of taxa summary plots?" truevalue="--suppress_taxa_summary" + falsevalue="" checked="False" help="(--suppress_taxa_summary)" /> + + <param name="suppress_beta_diversity" type="boolean" label="Suppress + beta diversity analyses?" truevalue="--suppress_beta_diversity" + falsevalue="" checked="False" help="(--suppress_beta_diversity)" /> + + <param name="suppress_alpha_diversity" type="boolean" label="Suppress + alpha diversity analyses?" truevalue="--suppress_alpha_diversity" + falsevalue="" checked="False" help="(--suppress_alpha_diversity)" /> + + <param name="suppress_group_significance" type="boolean" label="Suppress + OTU/category significance analysis?" truevalue="--suppress_group_significance" + falsevalue="" checked="False" help="(--suppress_group_significance)" /> + + <param name="categories" type="text" value="" label="Metadata category or + categories to compare for categorical analyses (optional)" + help="The column headers in the mapping file should be passed as a + comma-separated list. By default, no categorical analyses are performed + (-c/--categories)" optional="true"/> + + </inputs> + + <outputs> + <data name="html_report" format="html" + label="${tool.name} on ${on_string}: Core diversity report" /> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ + +**What it does?** + +This tool calculates alpha diversity, or within-sample diversity, using an +OTU table. + +The resulting file(s) is a tab-delimited text file, where the columns correspond +to alpha diversity metrics and the rows correspond to samples and their calculated +diversity measurements. When a folder is given as input (-i), the script processes +every otu table file in the given folder, and creates a corresponding file in the +output directory. + +More information about this tool is available on +`QIIME documentation <http://qiime.org/scripts/alpha_diversity.html>`_. + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/count_seqs.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,61 @@ +<tool id="count_seqs" name="Count sequences" version="1.9.1"> + + <description></description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <version_command><![CDATA[ + count_seqs.py --version + ]]> + </version_command> + + <command><![CDATA[ + count_seqs.py + + -i $input_files + -o $output_fp + + $suppress_errors + ]]> + </command> + + <inputs> + <param name="input_files" type="data" format="fasta" + label="Input sequence file" help="(-i/--input_fps)" + multiple="True"/> + + <param name="suppress_errors" type="boolean" label="Suppress warnings + about missing files?" truevalue="--suppress_errors" falsevalue="" + checked="False" help="(--suppress_errors)" /> + </inputs> + + <outputs> + <data name="output_fp" format="txt"/> + </outputs> + + <tests> + <test> + <param name="input_files" + value="split_fastq_libraries_sequences.fasta" /> + <output name="output_fp" value="" /> + </test> + </tests> + + <help><![CDATA[ +**What it does** + +This tool counts the sequences in fasta files + +More information about this tool is available on +`QIIME documentation <http://qiime.org/scripts/count_seqs.html>`_. +]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/filter_alignment.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,102 @@ +<tool id="qiime_filter_alignment" name="filter alignment" version="1.9.1galaxy1"> + <description>Filter sequence alignment by removing highly variable + regions</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <command><![CDATA[ + filter_alignment.py -i $input_fasta_file -o filter_alignment_output + #if str($lane_mask_fp) != 'None': + -m $lane_mask_fp + #end if + + #if $suppress_lane_mask_filter: + -s + #end if + + #if $allowed_gap_frac: + -g $allowed_gap_frac + #end if + + #if $remove_outliers: + -r + #end if + + #if $threshold: + -t $threshold + #end if + + #if $entropy_threshold: + -e $entropy_threshold + #end if + ]]> + </command> + + <inputs> + <param label="-i/--input_fasta_file: the input fasta file containing the + alignment" name="input_fasta_file" optional="False" type="data"/> + <param default="None" label="-m/--lane_mask_fp: path to lane mask file + [default: 16S alignment lane mask (Lane, D.J. 1991)]" + name="lane_mask_fp" optional="True" type="data"/> + <param label="-s/--suppress_lane_mask_filter: suppress lane mask filtering + [default: False]" name="suppress_lane_mask_filter" selected="False" + type="boolean"/> + <param default="0.999999" label="-g/--allowed_gap_frac: gap filter threshold, + filters positions which are gaps in > allowed_gap_frac of the + sequences [default: 0.999999]" name="allowed_gap_frac" optional="True" + type="float"/> + <param label="-r/--remove_outliers: remove seqs very dissimilar to the + alignment consensus (see --threshold). [default: False]" + name="remove_outliers" selected="False" type="boolean"/> + <param default="3.0" label="-t/--threshold: with -r, remove seqs whose + dissimilarity to the consensus sequence is approximately > x standard + deviations above the mean of the sequences [default: 3.0]" name="threshold" + optional="True" type="float"/> + <param default="None" label="-e/--entropy_threshold: Percent threshold for + removing base positions with the highest entropy, expressed as a + fraction between 0 and 1. For example, if 0.10 were specified, the + top 10% most entropic base positions would be filtered. If this + value is used, any lane mask supplied will be ignored. Entropy filtering + occurs after gap filtering. [default: None]" name="entropy_threshold" + optional="True" type="float"/> + </inputs> + + <outputs> + <data format="fasta" name="pfiltered.fasta" + from_work_dir="filter_alignment_output/*pfiltered.fasta" + label="filtered_alignement.fasta"/> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ +**What it does** + +This script should be applied to generate a useful tree when aligning against a +template alignment (e.g., with PyNAST). This script will remove positions which +are gaps in every sequence (common for PyNAST, as typical sequences cover only +200-400 bases, and they are being aligned against the full 16S gene). Additionally, +the user can supply a lanemask file, that defines which positions should included +when building the tree, and which should be ignored. Typically, this will differentiate +between non-conserved positions, which are uninformative for tree building, and +conserved positions which are informative for tree building. FILTERING ALIGNMENTS +WHICH WERE BUILT WITH PYNAST AGAINST THE GREENGENES CORE SET ALIGNMENT SHOULD BE +CONSIDERED AN ESSENTIAL STEP. + +The output of filter_alignment.py consists of a single FASTA file, which ends with +"pfiltered.fasta", where the "p" stands for positional +filtering of the columns. + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/filter_fasta.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,102 @@ +<tool id="qiime_filter_fasta" name="filter fasta" version="1.9.1galaxy1"> + <description>This script can be applied to remove sequences from a fasta or + fastq file based on input criteria.</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <command> +<![CDATA[ + filter_fasta.py + -f $input_fasta_fp + -o $output_fasta_fp + #if str($otu_map) != 'None': + -m $otu_map + #end if + + #if str($seq_id_fp) != 'None': + -s $seq_id_fp + #end if + + #if str($biom_fp) != 'None': + -b $biom_fp + #end if + + #if str($subject_fasta_fp) != 'None': + -a $subject_fasta_fp + #end if + + #if str($seq_id_prefix): + -p $seq_id_prefix + #end if + + #if str($sample_id_fp) != 'None': + --sample_id_fp=$sample_id_fp + #end if + + #if $negate: + -n + #end if + + #if str($mapping_fp) != 'None': + --mapping_fp=$mapping_fp + #end if + + #if str($valid_states): + --valid_states=$valid_states + #end if +]]> + </command> + + <inputs> + <param label="-f/--input_fasta_fp: path to the input fasta file" + name="input_fasta_fp" optional="False" type="data"/> + <param label="-m/--otu_map: an OTU map where sequences ids are those + which should be retained" name="otu_map" optional="True" type="data"/> + <param label="-s/--seq_id_fp: A list of sequence identifiers (or tab-delimited + lines with a seq identifier in the first field) which should be retained" + name="seq_id_fp" optional="True" type="data"/> + <param label="-b/--biom_fp: A biom file where otu identifiers should be + retained" name="biom_fp" optional="True" type="data"/> + <param label="-a/--subject_fasta_fp: A fasta file where the seq ids should + be retained." name="subject_fasta_fp" optional="True" type="data"/> + <param label="-p/--seq_id_prefix: keep seqs where seq_id starts with this + prefix" name="seq_id_prefix" optional="True" type="text"/> + <param label="--sample_id_fp: keep seqs where seq_id starts with a sample + id listed in this file" name="sample_id_fp" optional="True" + type="data"/> + <param label="-n/--negate: discard passed seq ids rather than keep passed + seq ids [default: False]" name="negate" selected="False" + type="boolean"/> + <param label="--mapping_fp: mapping file path (for use with --valid_states) + [default: None]" name="mapping_fp" optional="True" type="data"/> + <param label="--valid_states: description of sample ids to retain (for use + with --mapping_fp) [default: None]" name="valid_states" optional="True" + type="text"/> + </inputs> + + <outputs> + <data format="txt" name="output_fasta_fp"/> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help> +<![CDATA[ + +**What it does** + + + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/filter_otus_from_otu_table.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,80 @@ +<tool id="qiime_filter_otus_from_otu_table" name="filter otus from otu table" + version="1.9.1galaxy1"> + + <description>Filter OTUs from an OTU table based on their observation counts + or identifier.</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <command> +<![CDATA[ + filter_otus_from_otu_table.py + -i $input_fp + -o $output_fp + #if $negate_ids_to_exclude: + --negate_ids_to_exclude + #end if + + #if $min_count: + -n $min_count + #end if + + #if $min_count_fraction: + --min_count_fraction=$min_count_fraction + #end if + + #if $max_count: + -x $max_count + #end if + + #if $min_samples: + -s $min_samples + #end if + + #if $max_samples: + -y $max_samples + #end if + + #if str($otu_ids_to_exclude_fp) != 'None': + -e $otu_ids_to_exclude_fp + #end if +]]> + </command> + + <inputs> + <param label="-i/--input_fp: the input otu table filepath in biom format" name="input_fp" optional="False" type="data"/> + <param label="--negate_ids_to_exclude: keep OTUs in otu_ids_to_exclude_fp rather than discard them [default:False] " name="negate_ids_to_exclude" selected="False" type="boolean"/> + <param default="0" label="-n/--min_count: the minimum total observation count of an otu for that otu to be retained [default: 0]" name="min_count" optional="True" type="integer"/> + <param default="0" label="--min_count_fraction: fraction of the total observation (sequence) count to apply as the minimum total observation count of an otu for that otu to be retained. this is a fraction, not percent, so if you want to filter to 1%, you specify 0.01. [default: 0]" name="min_count_fraction" optional="True" type="float"/> + <param default="inf" label="-x/--max_count: the maximum total observation count of an otu for that otu to be retained [default: infinity]" name="max_count" optional="True" type="integer"/> + <param default="0" label="-s/--min_samples: the minimum number of samples an OTU must be observed in for that otu to be retained [default: 0]" name="min_samples" optional="True" type="integer"/> + <param default="inf" label="-y/--max_samples: the maximum number of samples an OTU must be observed in for that otu to be retained [default: infinity]" name="max_samples" optional="True" type="integer"/> + <param default="None" label="-e/--otu_ids_to_exclude_fp: file containing list of OTU ids to exclude: can be a text file with one id per line, a text file where id is the first value in a tab-separated line, or can be a fasta file (extension must be .fna or .fasta) [default: None]" name="otu_ids_to_exclude_fp" optional="True" type="data"/> + </inputs> + + <outputs> + <data format="txt" name="output_fp" label="Filter_otus_from_otu_table" /> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help> +<![CDATA[ + +**What it does** + + + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/filter_samples_from_otu_table.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,85 @@ +<tool id="qiime_filter_samples_from_otu_table" name="filter samples from otu table" + version="1.9.1galaxy1"> + + <description>Filters samples from an OTU table on the basis of the number of + observations in that sample, or on the basis of sample metadata. Mapping + file can also be filtered to the resulting set of sample ids. + </description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <command> +<![CDATA[ + filter_samples_from_otu_table.py + -i $input_fp + -o $output_fp + #if str($mapping_fp) != 'None': + -m $mapping_fp + --output_mapping_fp=$output_mapping_fp + #end if + #if str($sample_id_fp) != 'None': + --sample_id_fp=$sample_id_fp + #end if + + #if str($valid_states): + -s $valid_states + #end if + + #if $min_count: + -n $min_count + #end if + + #if $max_count: + -x $max_count + #end if +]]> + </command> + + <inputs> + <param label="-i/--input_fp: the input otu table filepath in biom format" + name="input_fp" optional="False" type="data"/> + <param label="-m/--mapping_fp: path to the map file [default: None]" + name="mapping_fp" optional="True" type="data"/> + <param label="--sample_id_fp: Path to file listing sample ids to keep. + Valid formats for the file are: 1) any white space, newline, or tab + delimited list of samples, 2) a mapping file with samples in the first + column [default: None]" name="sample_id_fp" optional="True" + type="data"/> + <param label="-s/--valid_states: string describing valid states (e.g. + 'Treatment:Fasting') [default: None]" name="valid_states" + optional="True" type="text"/> + <param default="0" label="-n/--min_count: the minimum total observation + count in a sample for that sample to be retained [default: 0]" + name="min_count" optional="True" type="integer"/> + <param default="inf" label="-x/--max_count: the maximum total observation + count in a sample for that sample to be retained [default: infinity]" + name="max_count" optional="True" type="integer"/> + </inputs> + + <outputs> + <data format="txt" name="output_fp" label="Filtered_samples.biom" /> + <data format="txt" name="output_mapping_fp" label="Filtered_samples_mapping.txt"/> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help> +<![CDATA[ + +**What it does** + + + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/filter_taxa_from_otu_table.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,65 @@ +<tool id="qiime_filter_taxa_from_otu_table" name="filter taxa from otu table" + version="1.9.1galaxy1"> + <description>Filter taxa from an OTU table</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <command> +<![CDATA[ + filter_taxa_from_otu_table.py + -i $input_otu_table_fp + -o $output_otu_table_fp + #if str($positive_taxa): + -p $positive_taxa + #end if + + #if str($negative_taxa): + -n $negative_taxa + #end if + + #if str($metadata_field): + --metadata_field=$metadata_field + #end if +]]> + </command> + + <inputs> + <param label="-i/--input_otu_table_fp: the input otu table filepath" + name="input_otu_table_fp" optional="False" type="data"/> + <param label="-p/--positive_taxa: comma-separated list of taxa to retain + [default: None; retain all taxa]" name="positive_taxa" optional="True" + type="text"/> + <param label="-n/--negative_taxa: comma-separated list of taxa to discard + [default: None; retain all taxa]" name="negative_taxa" optional="True" + type="text"/> + <param default="taxonomy" label="--metadata_field: observation metadata + identifier to filter based on [default: taxonomy]" name="metadata_field" + optional="True" type="text"/> + </inputs> + + <outputs> + <data format="txt" name="output_otu_table_fp"/> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help> +<![CDATA[ + +**What it does** + + + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/jackknifed_beta_diversity.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,121 @@ +<tool id="qiime_jackknifed_beta_diversity" name="Perform jackknifed UPGMA + clustering" version="1.9.1"> + + <description>and building jackknifed PCoA plots</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <version_command><![CDATA[ + jackknifed_beta_diversity.py --version + ]]> + </version_command> + + <stdio> + <regex match="VisibleDeprecationWarning" + source="both" + level="warning" + description="VisibleDeprecationWarning on numpy for `rank`" /> + <regex match="RuntimeWarning: The result contains negative eigenvalues" + source="both" + level="warning" + description="Presence of negative eigenvalues" /> + <regex match="Error" + source="both" + level="error" + description="Error" /> + </stdio> + + <command> +<![CDATA[ + jackknifed_beta_diversity.py + -i $otu_table_fp + -m $mapping_fp + -o jackknifed_beta_diversity + -e $seqs_per_sample + + #if $tree_fp + -t $tree_fp + #end if + + #if $parameter_fp + -p $parameter_fp + #end if + + --master_tree $master_tree + + $parallel + -O "\${GALAXY_SLOTS:-4}" + && + + python $__tool_directory__/jackknifed_beta_diversity_through_plots_html_generation.py + --data_directory jackknifed_beta_diversity + --html_file $jackknifed_beta_diversity_report + --html_dir $jackknifed_beta_diversity_report.files_path + + ]]> + </command> + + <inputs> + <param name="otu_table_fp" type="data" format="tabular,txt,tsv,biom" + label="OTU table" help="(-i/--otu_table_fp)" + multiple="True"/> + + <param name="mapping_fp" type="data" format="tabular,txt,tsv" + label="Mapping file" help="(-m/--mapping_fp)" + multiple="True"/> + + <param name="seqs_per_sample" type="integer" value="" label="Number of + sequences to include in each jackknifed subset" + help="(-e/--seqs_per_sample)"/> + + <param name="tree_fp" type="data" format="txt" + label="Tree file (Optional)" help="It is required for phylogenetic + measures (-t/--tree_fp)" + optional="True"/> + + <param name="parameter_fp" type="data" format="txt" + label="Parameter file (Optional)" help="It specifies changes to the + default behavior, e.g. beta diversity metrics (-p/--parameter_fp)" + optional="true"/> + + <param name="master_tree" type="select" + label="Method for computing master trees in jackknife analysis" + help="(--master_tree)"> + <option value="consensus" selected="True">Consensus of trees from jackknifed otu tables</option> + <option value="full">Tree generated from input (unsubsambled) otu table</option> + </param> + + <param name="parallel" type="boolean" label="Run in parallel where + available?" truevalue="--parallel" falsevalue="" checked="true" + help="(-a, --parallel)" /> + + </inputs> + + <outputs> + <data format="html" name="jackknifed_beta_diversity_report" + label="${tool.name} on ${on_string}: Report"/> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ +**What it does** + +This tool performs jackknifing (repeatedly resampling a subset of the available data from each sample) to measure robustness of individual UPGMA clusters and clusters in PCoA plots. + +More information about this tool is available on +`QIIME documentation <http://qiime.org/scripts/jackknifed_beta_diversity.html>`_. + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/jackknifed_beta_diversity_through_plots_html_generation.py Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,92 @@ +#!/usr/bin/env python +# -*- coding: utf-8 -*- + +import sys +import os +import argparse +import re + + +def generate_index_html(dir_list, args): + with open('index.html','w') as index_html_file: + index_html_file.write('<html>\n') + index_html_file.write('\t<head><title>Jackknifed beta diversity results</title></head>\n') + index_html_file.write('\t<body>\n') + index_html_file.write('\t\t<a href="http://www.qiime.org" target="_blank"><img src="http://qiime.org/_static/wordpressheader.png" alt="www.qiime.org""/></a>\n') + index_html_file.write('\t\t<p>\n') + index_html_file.write('\t\t\tBeta diversity metrics\n') + index_html_file.write('\t\t\t<ul>\n') + + for directory in dir_list: + if directory == 'rarefaction': + index_html_file.write('\t\t\t\t<li>' + directory) + index_html_file.write('\t\t\t\t<ul>\n') + for file_name in os.listdir(args.data_directory + '/' + directory): + index_html_file.write('\t\t\t\t\t<li><a href="' + directory + + '/' + file_name + '">' + file_name + '</a></li>\n') + index_html_file.write('\t\t\t\t</ul>\n') + index_html_file.write('\t\t\t\t</li>\n') + + elif directory != 'unrarefied_bdiv': + index_html_file.write('\t\t\t\t<li>' + directory) + index_html_file.write('\t\t\t\t<ul>\n') + + index_html_file.write('\t\t\t\t\t<li>Rare distance matrix\n') + index_html_file.write('\t\t\t\t\t<ul>\n') + for file_name in os.listdir(args.data_directory + '/' + directory + '/rare_dm'): + index_html_file.write('\t\t\t\t\t<li><a href="' + directory + + '/rare_dm/' + file_name + '">' + file_name + '</a></li>\n') + index_html_file.write('\t\t\t\t\t</ul>\n') + index_html_file.write('\t\t\t\t\t</li>\n') + + index_html_file.write('\t\t\t\t\t<li>Rare UPGMA tree\n') + index_html_file.write('\t\t\t\t\t<ul>\n') + for file_name in os.listdir(args.data_directory + '/' + directory + '/rare_upgma'): + index_html_file.write('\t\t\t\t\t<li><a href="' + directory + + '/rare_upgma/' + file_name + '">' + file_name + '</a></li>\n') + index_html_file.write('\t\t\t\t\t</ul>\n') + index_html_file.write('\t\t\t\t\t</li>\n') + + index_html_file.write('\t\t\t\t\t<li>PCoA\n') + index_html_file.write('\t\t\t\t\t<ul>\n') + for file_name in os.listdir(args.data_directory + '/' + directory + '/pcoa'): + index_html_file.write('\t\t\t\t\t<li><a href="' + directory + + '/pcoa/' + file_name + '">' + file_name + '</a></li>\n') + index_html_file.write('\t\t\t\t\t</ul>\n') + index_html_file.write('\t\t\t\t\t</li>\n') + + index_html_file.write('\t\t\t\t\t<li><a href="' + directory + + '/emperor_pcoa_plots/index.html">PCoA plots</a></td></li>\n') + + index_html_file.write('\t\t\t\t</ul>\n') + index_html_file.write('\t\t\t\t</li>\n') + + index_html_file.write('\t\t\t</ul>\n') + index_html_file.write('\t\t</p>\n') + index_html_file.write('\t</body>\n') + index_html_file.write('</html>\n') + +def build_html(args): + os.mkdir(args.html_dir) + + dir_list = [ name for name in os.listdir(args.data_directory) if + os.path.isdir(os.path.join(args.data_directory, name)) ] + + generate_index_html(dir_list, args) + + os.system('cp -r index.html ' + args.html_dir) + + for directory in dir_list: + os.system('cp -r ' + args.data_directory + '/' + directory + ' ' + + args.html_dir) + + os.system('mv ' + args.html_dir + '/index.html ' + args.html_file) + +if __name__ == '__main__': + parser = argparse.ArgumentParser() + parser.add_argument('--data_directory', required=True) + parser.add_argument('--html_file', required=True) + parser.add_argument('--html_dir', required=True) + args = parser.parse_args() + + build_html(args)
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,11 @@ +<?xml version="1.0" ?> +<macros> + <xml name="requirements"> + <requirements> + <requirement type="package" version="1.9.1">qiime</requirement> + </requirements> + </xml> + <xml name="citations"> + <citation type="doi">10.1038/nmeth.f.303</citation> + </xml> +</macros>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/make_otu_heatmap.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,167 @@ +<tool id="qiime_make_otu_heatmap" name="make otu heatmap" version="1.9.1galaxy1"> + <description>Plot heatmap of OTU table</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <command> +<![CDATA[ + make_otu_heatmap.py + -i $otu_table_fp + -o make_otu_heatmap_output + + #if str($otu_tree) != 'None': + -t $otu_tree + #end if + + #if str($map_fname) != 'None': + -m $map_fname + #end if + + #if str($category): + -c $category + #end if + + #if str($sample_tree) != 'None': + -s $sample_tree + #end if + + #if $no_log_transform: + --no_log_transform + #end if + + #if $suppress_row_clustering: + --suppress_row_clustering + #end if + + #if $suppress_column_clustering: + --suppress_column_clustering + #end if + + #if $absolute_abundance: + --absolute_abundance + #end if + + #if str($ad.select) == "yes": + #if $ad.color_scheme: + --color_scheme $ad.color_scheme + #end if + + #if $ad.width: + --width $ad.width + #end if + + #if $ad.height: + --height $ad.height + #end if + + #if $ad.dpi: + --dpi $ad.dpi + #end if + + #if $ad.obs_md_category: + --obs_md_category $ad.obs_md_category + #end if + + #if $ad.obs_md_level: + --obs_md_level $ad.obs_md_level + #end if + #end if +]]> + + </command> + + <inputs> + <param label="-i/--otu_table_fp: path to the input OTU table (i.e., the + output from make_otu_table.py)" name="otu_table_fp" optional="False" + type="data"/> + <param default="None" label="-t/--otu_tree: Tree file to be used for + sorting OTUs in the heatmap" name="otu_tree" optional="True" + type="data"/> + <param default="None" label="-m/--map_fname: Metadata mapping file to be + used for sorting Samples in the heatmap." name="map_fname" optional="True" + type="data"/> + <param default="None" label="-c/--category: Metadata category for sorting + samples. Samples will be clustered within each category level using + euclidean UPGMA." name="category" optional="True" type="text"/> + <param default="None" label="-s/--sample_tree: Tree file to be used for + sorting samples (e.g, output from upgma_cluster.py). If both this and + the sample mapping file are provided, the mapping file is ignored." + name="sample_tree" optional="True" type="data"/> + <param label="--no_log_transform: Data will not be log-transformed. Without + this option, all zeros will be set to a small value (default is 1/2 + the smallest non-zero entry). Data will be translated to be non-negative + after log transform, and num_otu_hits will be set to 0." + name="no_log_transform" selected="False" type="boolean"/> + <param label="--suppress_row_clustering: No UPGMA clustering of OTUs (rows) + is performed. If --otu_tree is provided, this flag is ignored." + name="suppress_row_clustering" selected="False" type="boolean"/> + <param label="--suppress_column_clustering: No UPGMA clustering of Samples + (columns) is performed. If --map_fname is provided, this flag is ignored." + name="suppress_column_clustering" selected="False" type="boolean"/> + <param label="--absolute_abundance: Do not normalize samples to sum to 1. + [default False]" name="absolute_abundance" selected="False" + type="boolean"/> + + <conditional name="ad"> + <param name="select" type="select" label="Show advanced options"> + <option value="no" selected="True">No</option> + <option value="yes">Yes</option> + </param> + <when value="no"> + </when> + <when value="yes"> + <param default="YlGn" label="Color scheme for figure. see http://matplotlib.org/examples/color/colormaps_reference.html for choices [default: YlGn]" + name="color_scheme" optional="True" type="text"/> + <param default="5" label="Width of the figure in inches [default: 5]" + name="width" optional="True" type="text"/> + <param default="5" label="Height of the figure in inches [default: 5]" + name="height" optional="True" type="text"/> + <param label="Resolution of the figure in dots per inch [default: + value of savefig.dpi in matplotlibrc file]" name="dpi" + optional="True" type="text"/> + <param label="Observation metadata category to plot [default: taxonomy]" + name="obs_md_category" optional="True" type="data"/> + <param label="The level of observation metadata to plot for hierarchical + metadata [default: lowest level]" name="obs_md_level" optional="True" + type="data"/> + </when> + </conditional> + </inputs> + + <outputs> + <data format="pdf" from_work_dir="make_otu_heatmap_output" name="heatmap_OTU" + label="heatmap_OTU.pdf"/> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ + +**What it does** + +This script visualizes an OTU table as a heatmap where each row corresponds to an +OTU and each column corresponds to a sample. The higher the relative abundance of +an OTU in a sample, the more intense the color at the corresponsing position in +the heatmap. By default, the OTUs (rows) will be clustered by UPGMA hierarchical +clustering, and the samples (columns) will be presented in the order in which +they appear in the OTU table. Alternatively, the user may supply a tree to sort +the OTUs (rows) or samples (columns), or both. The user may also pass in a mapping +file for sorting samples. If the user passes in a mapping file and a metadata +category, samples (columns) will be grouped by category value and subsequently +clustered within each group. +A single output file is created containing the heatmap of the OTU table (a PDF +file by default). + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/make_otu_table.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,65 @@ +<tool id="qiime_make_otu_table" name="make otu table" version="1.9.1galaxy1"> + + <description>Make OTU table</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <command> +<![CDATA[ + make_otu_table.py + -i $otu_map_fp + -o $output_biom_fp + #if str($taxonomy) != 'None': + -t $taxonomy + #end if + + #if str($exclude_otus_fp) != 'None': + -e $exclude_otus_fp + #end if +]]> + </command> + + <inputs> + <param label="-i/--otu_map_fp: path to the input OTU map (i.e., the output + from pick_otus.py)" name="otu_map_fp" optional="False" type="data"/> + <param label="-t/--taxonomy: Path to taxonomy assignment, containing the + assignments of taxons to sequences (i.e., resulting txt file from + assign_taxonomy.py) [default: None]" name="taxonomy" optional="True" + type="data"/> + <param label="-e/--exclude_otus_fp: path to a file listing OTU identifiers + that should not be included in the OTU table (e.g., the output of + identify_chimeric_seqs.py) or a fasta file where seq ids should be + excluded (e.g., failures fasta file from align_seqs.py)" + name="exclude_otus_fp" optional="True" type="data"/> + </inputs> + + <outputs> + <data format="txt" name="output_biom_fp" label="otu_table.biom"/> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ + +**What it does** + +The script make_otu_table.py tabulates the number of times an OTU is found in each +sample, and adds the taxonomic predictions for each OTU in the last column if a +taxonomy file is supplied. +The output of make_otu_table.py is a biom file, where the columns correspond to +Samples and rows correspond to OTUs and the number of times a sample appears in +a particular OTU. + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/make_phylogeny.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,75 @@ +<tool id="qiime_make_phylogeny" name="Make phylogeny" version="1.9.1"> + + <description></description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <version_command><![CDATA[ + make_phylogeny.py --version + ]]> + </version_command> + + <stdio></stdio> + + <command> +<![CDATA[ + make_phylogeny.py + -i $input_fp + -o $result_fp + -t $tree_method + -l $log_fp + -r $root_method + ]]> + </command> + + <inputs> + <param name="input_fp" type="data" format="fasta" + label="Input fasta alignment" help="(-i/--input_fp)"/> + + <param name="tree_method" type="select" + label="Method for tree building" help="(-t/--tree_method)"> + <option value="clustalw">ClustalW</option> + <option value="raxml_v730">raxml_v730</option> + <option value="muscle">muscle</option> + <option value="fasttree" selected="true">fasttree</option> + <option value="clearcut">clearcut</option> + </param> + + <param name="root_method" type="select" + label="Method for choosing root of phylo tree" help="(-r/--root_method)"> + <option value="midpoint">Midpoint</option> + <option value="tree_method_default" selected="true">Default method of tree method</option> + </param> + + </inputs> + + <outputs> + <data format="txt" name="result_fp" + label="${tool.name} on ${on_string}: Tree"/> + <data format="txt" name="log_fp" + label="${tool.name} on ${on_string}: Log"/> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ +**What it does** + +This tool produces a tree from a multiple sequence alignment. Trees are constructed with a set of sequences representative of the OTUs + +More information about this tool is available on +`QIIME documentation <http://qiime.org/scripts/make_phylogeny.html>`_. + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/multiple_rarefactions.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,75 @@ +<tool id="qiime_multiple_rarefactions" name="Perform multiple subsamplings/rarefactions" version="1.9.1"> + <description>on an otu table</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <command> +<![CDATA[ + multiple_rarefactions.py -i $input_path -o multiple_rarefactions_output -m $min -x $max -s $step + + #if $numreps: + -n $numreps + #end if + + #if $lineages_included: + --lineages_included + #end if + + #if $keep_empty_otus: + -k + #end if + + #if $subsample_multinomial: + --subsample_multinomial + #end if +]]> + </command> + + <inputs> + <param label="-i/--input_path: Input OTU table filepath." + name="input_path" optional="False" type="data"/> + <param label="-m/--min: Minimum number of seqs/sample for rarefaction." + name="min" optional="False" type="integer" value="0"/> + <param label="-x/--max: Maximum number of seqs/sample (inclusive) for r + arefaction. " name="max" optional="False" type="integer" value="0"/> + <param label="-s/--step: Size of each steps between the min/max of + seqs/sample (e.g. min, min+step... for level = max)." name="step" + optional="False" type="integer" value="0"/> + <param default="10" label="-n/--num-reps: The number of iterations at + each step. [default: 10]" name="numreps" optional="True" type="integer"/> + <param label="--lineages_included: Retain taxonomic (lineage) information + for each OTU. Note: this will only work if lineage information is in + the input OTU table. [default: False]" name="lineages_included" + selected="False" type="boolean"/> + <param label="-k/--keep_empty_otus: Retain OTUs of all zeros, which are + usually omitted from the output OTU tables. [default: False]" + name="keep_empty_otus" selected="False" type="boolean"/> + <param label="--subsample_multinomial: subsample using subsampling with + replacement [default: False]" name="subsample_multinomial" + selected="False" type="boolean"/> + </inputs> + + <outputs> + <data format="tgz" name="multiple_rarefactions" label="Multiple_rarefaction.tgz" /> + <data format="tgz" name="output_dir" label="Alpha diversity.tgz" /> + <data format="text" name="alpha_param" label="alpha_param.txt" /> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ +To perform bootstrap, jackknife, and rarefaction analyses, the otu table must be subsampled (rarefied). This script rarefies, or subsamples, OTU tables. This does not provide curves of diversity by number of sequences in a sample. Rather it creates a series of subsampled OTU tables by random sampling (without replacement) of the input OTU table. Samples that have fewer sequences then the requested rarefaction depth for a given output otu table are omitted from those ouput otu tables. The pseudo-random number generator used for rarefaction by subsampling is NumPy’s default - an implementation of the Mersenne twister PRNG. + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/pick_open_reference_otus.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,295 @@ +<tool id="pick_open_reference_otus" name="Perform open-reference OTU picking" + version="1.9.1"> + + <description></description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <version_command><![CDATA[ + pick_open_reference_otus.py --version + ]]></version_command> + + <command><![CDATA[ + pick_open_reference_otus.py + + -i $input_seq_files + + -o otus + + -m $otu_picking_method + + #if str( $reference_sequences.reference_sequences_selector ) == 'history' + #set $ref = $reference_sequences.history_database + #else: + #set $data_table = dict([(_[0], _[2]) for _ in $reference_sequences.cached_database.input.options.tool_data_table.data]) + #set $db = $reference_sequences.cached_database.value + #set $ref = $data_table[$db] + #end if + -r $ref + + #if $prefilter_refseqs_fp.difference_test + #if str( $prefilter_refseqs_fp.prefilter_reference_sequences.reference_sequences_selector ) == 'history' + #set $ref = $prefilter_refseqs_fp.prefilter_reference_sequences.history_database + #else: + #set $data_table = dict([(_[0], _[2]) for _ in $prefilter_refseqs_fp.prefilter_reference_sequences.cached_database.input.options.tool_data_table.data]) + #set $db = $prefilter_refseqs_fp.prefilter_reference_sequences.cached_database.value + #set $ref = $data_table[$db] + #end if + --prefilter_refseqs_fp $ref + #end if + + -n $new_ref_set_id + + $parallel + -O "\${GALAXY_SLOTS:-4}" + + -s $percent_subsample + --prefilter_percent_id $prefilter_percent_id + + #if str($step1_otu_map_fp) != 'None': + --step1_otu_map_fp $step1_otu_map_fp + #end if + + #if str($step1_failures_fasta_fp) != 'None': + --step1_failures_fasta_fp=$step1_failures_fasta_fp + #end if + + --minimum_failure_threshold $minimum_failure_threshold + + $suppress_step4 + + --min_otu_size $min_otu_size + + $suppress_taxonomy_assignment + $suppress_align_and_tree + + && + + biom convert + -i otus/otu_table_mc2.biom + -o $output_otu_table + --to-tsv + --header-key taxonomy + + #if not $suppress_taxonomy_assignment + && + biom convert + -i otus/otu_table_mc2_w_tax.biom + -o $output_otu_table_taxonomy + --to-tsv + --header-key taxonomy + #end if + + #if not $suppress_align_and_tree + && + biom convert + -i otus/otu_table_mc_no_pynast_failures.biom + -o $output_otu_table_no_failures + --to-tsv + --header-key taxonomy + #end if + + ]]> + </command> + + <inputs> + <param name="input_seq_files" type="data" format="fasta,fastq" + label="Input sequence files" multiple="True" help="(-i/--input_fps)"/> + + <param name="otu_picking_method" label="The OTU picking method to use for + reference and de novo steps" type="select" + help="-m/--otu_picking_method"> + <option value="uclust" selected="True">uclust</option> + <option value="usearch61">usearch61</option> + <option value="sortmerna_sumaclust">sortmerna_sumaclust</option> + </param> + + <conditional name="reference_sequences"> + <param name="reference_sequences_selector" type="select" + label="Reference sequences to query" + help="(-r, --reference_fp)"> + <option value="cached" selected="True">Public databases</option> + <option value="history">Databases from your history</option> + </param> + <when value="cached"> + <param name="cached_database" label="QIIME databases of reference + sequences" type="select"> + <options from_data_table="qiime_reference_db" /> + </param> + </when> + <when value="history"> + <param name="history_database" type="data" format="fasta" + label="Reference databases"/> + </when> + </conditional> + + <conditional name="prefilter_refseqs_fp"> + <param name="difference_test" type="boolean" label="Use different + reference sequences for the prefilter?" checked="False" truevalue="true" + falsevalue="false" help="(--prefilter_refseqs_fp)" /> + <when value="true"> + <conditional name="prefilter_reference_sequences"> + <param name="reference_sequences_selector" type="select" + label="Reference sequences to query" > + <option value="cached" selected="True">Public databases</option> + <option value="history">Databases from your history</option> + </param> + <when value="cached"> + <param name="cached_database" label="QIIME databases of reference + sequences" type="select"> + <options from_data_table="qiime_reference_db" /> + </param> + </when> + <when value="history"> + <param name="history_database" type="data" format="fasta" + label="Reference databases"/> + </when> + </conditional> + </when> + <when value="false" /> + </conditional> + + <param name="new_ref_set_id" type="text" value="New" label="Unique + identifier for OTUs that get created in this ref set" + help="This is useful to support combining of reference sets + (-n/--new_ref_set_id)"/> + + <param name="parallel" type="boolean" label="Run in parallel where + available?" truevalue="--parallel" falsevalue="" checked="False" + help="(-a, --parallel)" /> + + <param name="percent_subsample" type="float" value="0.001" min="0" max="1" + label="Percent of failure sequences to include in the subsample to + cluster de novo" help="Large numbers should give more comprehensive + results but will be slower(-s/--percent_subsample)"/> + + <param name="prefilter_percent_id" type="float" value="0.0" min="0" max="1" + label="Percent of sequence pre-cluster against the reference" + help="Any reads which fail to hit are discarded (a quality filter) + (--prefilter_percent_id)"/> + + <param name="step1_otu_map_fp" type="data" format="txt" label="Reference + OTU picking OTU map to avoid rebuilding if one has already been built + (optional)" optional="True" help="This must be an OTU map generated + by this workflow, not (for example) by pick_closed_reference_otus + (--step1_otu_map_fp)" /> + + <param name="step1_failures_fasta_fp" type="data" format="fasta" + label="Reference OTU picking failures fasta filepath, to avoid + rebuilding if one has already been built (optional)" optional="True" + help="This must be a failures file generated by this workflow, not + (for example) by pick_closed_reference_otus (--step1_failures_fasta_fp)" /> + + <param name="minimum_failure_threshold" type="integer" value="100000" + label="Minimum number of sequences that must fail to hit the reference + for subsampling to be performed" help="If fewer than this number of + sequences fail to hit the reference, the de novo clustering step will + run serially rather than invoking the subsampled open reference approach + to improve performance (--minimum_failure_threshold)"/> + + <param name="suppress_step4" type="boolean" label="Suppress the final de + novo OTU picking step?" truevalue="--suppress_step4" falsevalue="" + checked="False" help="It may be necessary for extremely large data + sets(--suppress_step4)" /> + + <param name="min_otu_size" type="integer" value="2" label="Minimum otu + size to retain the OTU" help="(--min_otu_size)"/> + + <param name="suppress_taxonomy_assignment" type="boolean" + label="Skip the taxonomy assignment step?" + truevalue="--suppress_taxonomy_assignment" falsevalue="" + checked="False" help="It results in an OTU table without taxonomy + (--suppress_taxonomy_assignment)" /> + + <param name="suppress_align_and_tree" type="boolean" + label="Skip the sequence alignment and tree-building steps?" + truevalue="--suppress_align_and_tree" falsevalue="" + checked="False" help="(--suppress_align_and_tree)" /> + </inputs> + + <outputs> + <data name="output_rep_set" format="fasta" + from_work_dir="otus/rep_set.fna" + label="${tool.name} on ${on_string}: OTU representative sequences"/> + <data name="output_ref_set" format="fasta" + from_work_dir="otus/new_refseqs.fna" + label="${tool.name} on ${on_string}: New reference sequences (OTU + + input reference sequences)"/> + <data name="output_otu_table" format="tsv" + label="${tool.name} on ${on_string}: OTU table"/> + <data name="output_otu_table_taxonomy" format="tsv" + label="${tool.name} on ${on_string}: OTU table with taxonomy"> + <filter>suppress_taxonomy_assignment is False</filter> + </data> + <data name="output_otu_table_no_failures" format="tsv" + label="${tool.name} on ${on_string}: OTU without sequences that + failed to align"> + <filter>suppress_align_and_tree is False</filter> + </data> + <data name="output_otu_map" format="txt" + from_work_dir="otus/final_otu_map_mc2.txt" + label="${tool.name} on ${on_string}: OTU map"/> + <data name="output_rep_set_tree" format="txt" + from_work_dir="otus/rep_set.tre" + label="${tool.name} on ${on_string}: representative set tree"> + <filter>suppress_align_and_tree is False</filter> + </data> + </outputs> + + <tests> + <test> + <param name="input_seq_files" + value="split_fastq_libraries_sequences.fasta"/> + <param name="otu_picking_method" value="uclust"/> + <param name="reference_sequences_selector" value="history"/> + <param name="history_database" value="gg_13_8_97_otus.fasta"/> + <param name="difference_test" value="false"/> + <param name="new_ref_set_id" value="New" /> + <param name="parallel" value="--parallel"/> + <param name="percent_subsample" value="0.001" /> + <param name="prefilter_percent_id" value="0.0" /> + <param name="minimum_failure_threshold" value="100000" /> + <param name="suppress_step4" value=""/> + <param name="min_otu_size" value="2" /> + <param name="suppress_taxonomy_assignment" value="" /> + <param name="suppress_align_and_tree" value="" /> + + <output name="output_rep_set" + value="pick_open_reference_otus_representative_sequences.fasta"/> + <output name="output_ref_set" + value="pick_open_reference_otus_reference_sequences.fasta"/> + <output name="output_otu_table" + value="pick_open_reference_otus_otu_table.txt"/> + <output name="output_otu_table_taxonomy" + value="pick_open_reference_otus_otu_table_with_taxo.txt" /> + <output name="output_otu_map" + value="pick_open_reference_otus_otu_map.txt"/> + <output name="output_otu_table_no_failures" + value="pick_open_reference_otus_otu_table_with_taxo_without_pynast_failure.txt" /> + <output name="output_rep_set_tree" + value="pick_open_reference_otus_representative_set_tree.txt" /> + </test> + </tests> + + <help><![CDATA[ + +**What it does** + +This tool performs open-reference OTU picking from sequences. + +This script is broken down into 4 possible OTU picking steps, and 2 steps +involving the creation of OTU tables and trees. + +More information about this tool is available on +`QIIME documentation <http://qiime.org/scripts/pick_open_reference_otus.html>`_. +]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/pick_otus.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,412 @@ +<tool id="qiime_pick_otus" name="pick otus" version="1.9.1galaxy1"> + + <description>OTU picking</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <command> +<![CDATA[ + pick_otus.py + -i $input_seqs_filepath + -o fastasplit + + #if str($methode.otu_picking_method) != 'None': + -m $methode.otu_picking_method + #end if + + #if str($methode.otu_picking_method) in ("uclust_ref","usearch_ref") : + -r $methode.refseqs_fp + #end if + + #if str($methode.otu_picking_method) in ("uclust","uclust_ref","usearch","usearch_ref","sumaclust") : + #if $methode.similarity: + -s $methode.similarity + #end if + #end if + + #if str($methode.otu_picking_method) == "sumaclust": + #if $methode.sumaclust_exact: + --sumaclust_exact + #end if + #end if + + #if str($methode.otu_picking_method) == "swarm": + #if $methode.swarm_resolution: + --swarm_resolution=$methode.swarm_resolution + #end if + #end if + + #if str($methode.otu_picking_method) in ("uclust","uclust_ref","usearch","usearch_ref"): + #if $methode.enable_rev_strand_match: + -z + #end if + #if str($methode.max_accepts): + --max_accepts=$methode.max_accepts + #end if + + #if str($methode.max_rejects): + --max_rejects=$methode.max_rejects + #end if + #end if + + #if str($methode.otu_picking_method) in ("uclust","uclust_ref"): + #if $methode.stepwords: + --stepwords=$methode.stepwords + #end if + #if $methode.suppress_presort_by_abundance_uclust: + -D + #end if + #end if + + #if str($methode.otu_picking_method) == "uclust": + #if $methode.optimal_uclust: + -A + #end if + #if $methode.exact_uclust: + -E + #end if + #end if + + #if str($methode.otu_picking_method) == "usearch": + #if $methode.percent_id_err: + -j $methode.percent_id_err + #end if + #if $methode.abundance_skew: + -a $methode.abundance_skew + #end if + #if str($methode.db_filepath) != 'None': + -f $methode.db_filepath + #end if + #if $methode.perc_id_blast: + --perc_id_blast=$methode.perc_id_blast + #end if + #if $methode.suppress_de_novo_chimera_detection: + -k + #end if + #end if + + #if str($methode.otu_picking_method) in ("sumaclust","swarm"): + #if str($methode.threads): + --threads=$methode.threads + #end if + #end if + + #if $prefix_prefilter_length: + -n $prefix_prefilter_length + #end if + + #if $prefix_length: + -p $prefix_length + #end if + + #if $suffix_length: + -u $suffix_length + #end if + + #if str($non_chimeras_retention): + -F $non_chimeras_retention + #end if +]]> + </command> + + <inputs> + <param label="-i/--input_seqs_filepath: Path to input sequences file" + name="input_seqs_filepath" optional="False" type="data"/> + <conditional name="methode"> + <param label="-m/--otu_picking_method: Method for picking OTUs. Valid + choices are: sortmerna, mothur, trie, uclust_ref, usearch, usearch_ref, + blast, usearch61, usearch61_ref, sumaclust, swarm, prefix_suffix, + cdhit, uclust. The mothur method requires an input file of aligned + sequences. usearch will enable the usearch quality filtering + pipeline. [default: uclust]" name="otu_picking_method" + optional="FALSE" type="select"> + <option selected="True" value="uclust">uclust</option> + <option value="uclust_ref">uclust_ref</option> + <option value="usearch">usearch</option> + <option value="usearch_ref">usearch_ref</option> + <option value="sumaclust">sumaclust</option> + <option value="swarm">swarm</option> + </param> + <when value="uclust_ref"> + <param default="/home12/caparmor/bioinfo/softs/sources/Qiime/qiime-1.9.0/python_modules/lib/python2.7/site-packages/qiime_default_reference-0.1.1-py2.7.egg/qiime_default_reference/gg_13_8_otus/rep_set/97_otus.fasta" + label="-r/--refseqs_fp: Path to reference sequences to search + against when using -m uclust_ref, -m usearch_ref [default: + /home12/caparmor/bioinfo/softs/sources/Qiime/qiime-1.9.0/python_modules/lib/python2.7/site-packages/qiime_default_reference-0.1.1-py2.7.egg/qiime_default_reference/gg_13_8_otus/rep_set/97_otus.fasta]" name="refseqs_fp" optional="True" type="data"/> + <param default="0.97" label="-s/--similarity: Sequence similarity + threshold (for blast, cdhit, uclust, uclust_ref, usearch, + usearch_ref, usearch61, usearch61_ref, sumaclust or sortmerna + [default: 0.97]" name="similarity" optional="True" type="float"/> + <param label="-z/--enable_rev_strand_match: Enable reverse strand + matching for uclust, uclust_ref, usearch, usearch_ref, usearch61, + or usearch61_ref otu picking, will double the amount of memory + used. [default: False]" name="enable_rev_strand_match" + selected="False" type="boolean"/> + <param label="-D/--suppress_presort_by_abundance_uclust: Suppress + presorting of sequences by abundance when picking OTUs with + uclust or uclust_ref [default: False]" + name="suppress_presort_by_abundance_uclust" selected="False" + type="boolean"/> + <param label="-C/--suppress_new_clusters: Suppress creation of new + clusters using seqs that don't match reference when using -m + uclust_ref, -m usearch61_ref, or -m usearch_ref [default: False]" + name="suppress_new_clusters" selected="False" type="boolean"/> + <param default="default" label="--max_accepts: max_accepts value + to uclust, uclust_ref, usearch61, and usearch61_ref. By default, + will use value suggested by method (uclust: 1, usearch61: 1) + [default: default]" name="max_accepts" optional="True" type="text"/> + <param default="default" label="--max_rejects: max_rejects value + for uclust, uclust_ref, usearch61, and usearch61_ref. With + default settings, will use value recommended by clustering + method used (uclust: 8, usearch61: 8 for usearch_fast_cluster + option, 32 for reference and smallmem options) [default: + default]" name="max_rejects" optional="True" type="text"/> + <param default="8" label="--stepwords: stepwords value to uclust + and uclust_ref [default: 8]" name="stepwords" optional="True" + type="integer"/> + </when> + <when value="usearch_ref"> + <param default="/home12/caparmor/bioinfo/softs/sources/Qiime/qiime-1.9.0/python_modules/lib/python2.7/site-packages/qiime_default_reference-0.1.1-py2.7.egg/qiime_default_reference/gg_13_8_otus/rep_set/97_otus.fasta" + label="-r/--refseqs_fp: Path to reference sequences to search + against when using -m blast, -m sortmerna, -m uclust_ref, -m + usearch_ref, or -m usearch61_ref [default: + /home12/caparmor/bioinfo/softs/sources/Qiime/qiime-1.9.0/python_modules/lib/python2.7/site-packages/qiime_default_reference-0.1.1-py2.7.egg/qiime_default_reference/gg_13_8_otus/rep_set/97_otus.fasta]" name="refseqs_fp" optional="True" type="data"/> + <param default="0.97" label="-s/--similarity: Sequence similarity + threshold (for blast, cdhit, uclust, uclust_ref, usearch, + usearch_ref, usearch61, usearch61_ref, sumaclust or sortmerna + [default: 0.97]" name="similarity" optional="True" type="float"/> + <param label="-z/--enable_rev_strand_match: Enable reverse strand + matching for uclust, uclust_ref, usearch, usearch_ref, usearch61, + or usearch61_ref otu picking, will double the amount of memory + used. [default: False]" name="enable_rev_strand_match" selected="False" + type="boolean"/> + <param label="-C/--suppress_new_clusters: Suppress creation of new + clusters using seqs that don't match reference when using -m + uclust_ref, -m usearch61_ref, or -m usearch_ref [default: False]" + name="suppress_new_clusters" selected="False" type="boolean"/> + <param default="default" label="--max_accepts: max_accepts value + to uclust, uclust_ref, usearch61, and usearch61_ref. By default, + will use value suggested by method (uclust: 1, usearch61: 1) + [default: default]" name="max_accepts" optional="True" type="text"/> + <param default="default" label="--max_rejects: max_rejects value + for uclust, uclust_ref, usearch61, and usearch61_ref. With + default settings, will use value recommended by clustering method + used (uclust: 8, usearch61: 8 for usearch_fast_cluster option, + 32 for reference and smallmem options) [default: default]" + name="max_rejects" optional="True" type="text"/> + </when> + <when value="usearch"> + <param default="0.97" value="0.97" label="-s/--similarity: Sequence + similarity threshold (for blast, cdhit, uclust, uclust_ref, + usearch, usearch_ref, usearch61, usearch61_ref, sumaclust or + sortmerna [default: 0.97]" name="similarity" optional="True" + type="float"/> + <param label="-z/--enable_rev_strand_match: Enable reverse strand + matching for uclust, uclust_ref, usearch, usearch_ref, usearch61, + or usearch61_ref otu picking, will double the amount of memory + used. [default: False]" name="enable_rev_strand_match" + selected="False" type="boolean"/> + <param default="default" label="--max_accepts: max_accepts value + to uclust, uclust_ref, usearch61, and usearch61_ref. By + default, will use value suggested by method (uclust: 1, + usearch61: 1) [default: default]" name="max_accepts" optional="True" + type="text"/> + <param default="default" label="--max_rejects: max_rejects value + for uclust, uclust_ref, usearch61, and usearch61_ref. With + default settings, will use value recommended by clustering + method used (uclust: 8, usearch61: 8 for usearch_fast_cluster + option, 32 for reference and smallmem options) [default: default]" + name="max_rejects" optional="True" type="text"/> + <param default="0.97" value="0.97" label="-j/--percent_id_err: + Percent identity threshold for cluster error detection with + usearch, expressed as a fraction between 0 and 1. [default: 0.97]" + name="percent_id_err" optional="True" type="float"/> + <param default="2.0" label="-a/--abundance_skew: Abundance skew + setting for de novo chimera detection with usearch. [default: 2.0]" + name="abundance_skew" optional="True" type="float"/> + <param default="None" label="-f/--db_filepath: Reference database + of fasta sequences for reference based chimera detection with + usearch. [default: None]" name="db_filepath" optional="True" + type="data"/> + <param default="0.97" value="0.97" label="--perc_id_blast: Percent + ID for mapping OTUs created by usearch back to original sequence + IDs [default: 0.97]" name="perc_id_blast" optional="True" + type="float"/> + <param label="-k/--suppress_de_novo_chimera_detection: Suppress + de novo chimera detection in usearch. [default: False]" + name="suppress_de_novo_chimera_detection" selected="False" + type="boolean"/> + <param label="--usearch_fast_cluster: Use fast clustering option + for usearch or usearch61_ref with new clusters. + --enable_rev_strand_match can not be enabled with this option, + and the only valid option for usearch61_sort_method is 'length'. + This option uses more memory than the default option for de novo + clustering. [default: False]" name="usearch_fast_cluster" + selected="False" type="boolean"/> + </when> + <when value="sumaclust"> + <param default="0.97" value="0.97" label="-s/--similarity: Sequence + similarity threshold (for blast, cdhit, uclust, uclust_ref, + usearch, usearch_ref, usearch61, usearch61_ref, sumaclust or + sortmerna [default: 0.97]" name="similarity" optional="True" + type="float"/> + <param label="--sumaclust_exact: A sequence is assigned to the best + matching seed rather than the first matching seed passing the + similarity threshold [default: False]" name="sumaclust_exact" + selected="False" type="boolean"/> + <param default="1" label="--threads: Specify number of threads (1 + thread per core) to be used for usearch61, sortmerna, sumaclust and swarm commands that utilize multithreading. [default: 1]" name="threads" optional="True" type="text"/> + </when> + <when value="swarm"> + <param default="1" label="--swarm_resolution: Maximum number of + differences allowed between two amplicons, meaning that two + amplicons will be grouped if they have integer (or less) + differences (see Swarm manual at https://github.com/torognes/swarm + for more details). [default: 1]" name="swarm_resolution" + optional="True" type="integer"/> + <param default="1" label="--threads: Specify number of threads (1 + thread per core) to be used for usearch61, sortmerna, sumaclust + and swarm commands that utilize multithreading. [default: 1]" + name="threads" optional="True" type="text"/> + </when> + <when value="uclust"> + <param default="0.97" value="0.97" label="-s/--similarity: Sequence + similarity threshold (for blast, cdhit, uclust, uclust_ref, + usearch, usearch_ref, usearch61, usearch61_ref, sumaclust or + sortmerna [default: 0.97]" name="similarity" optional="True" + type="float"/> + <param label="-z/--enable_rev_strand_match: Enable reverse strand + matching for uclust, uclust_ref, usearch, usearch_ref, usearch61, + or usearch61_ref otu picking, will double the amount of memory + used. [default: False]" name="enable_rev_strand_match" selected="False" + type="boolean"/> + <param label="-A/--optimal_uclust: Pass the --optimal flag to uclust + for uclust otu picking. [default: False]" name="optimal_uclust" + selected="False" type="boolean"/> + <param label="-D/--suppress_presort_by_abundance_uclust: Suppress + presorting of sequences by abundance when picking OTUs with + uclust or uclust_ref [default: False]" + name="suppress_presort_by_abundance_uclust" selected="False" + type="boolean"/> + <param default="default" label="--max_accepts: max_accepts value + to uclust, uclust_ref, usearch61, and usearch61_ref. By default, + will use value suggested by method (uclust: 1, usearch61: 1) + [default: default]" name="max_accepts" optional="True" + type="text"/> + <param default="default" label="--max_rejects: max_rejects value + for uclust, uclust_ref, usearch61, and usearch61_ref. With + default settings, will use value recommended by clustering + method used (uclust: 8, usearch61: 8 for usearch_fast_cluster + option, 32 for reference and smallmem options) [default: default]" + name="max_rejects" optional="True" type="text"/> + <param default="8" label="--stepwords: stepwords value to uclust + and uclust_ref [default: 8]" name="stepwords" optional="True" + type="integer"/> + <param label="-E/--exact_uclust: Pass the --exact flag to uclust + for uclust otu picking. [default: False]" name="exact_uclust" + selected="False" type="boolean"/> + </when> + </conditional> + + <param default="None" label="-n/--prefix_prefilter_length: Prefilter data + so seqs with identical first prefix_prefilter_length are automatically + grouped into a single OTU. This is useful for large sequence collections + where OTU picking doesn't scale well [default: None; 100 is a good value]" + name="prefix_prefilter_length" optional="True" type="integer"/> + <param default="50" label="-p/--prefix_length: Prefix length when using + the prefix_suffix otu picker; WARNING: CURRENTLY DIFFERENT FROM + prefix_prefilter_length (-n)! [default: 50]" name="prefix_length" + optional="True" type="integer"/> + <param default="50" label="-u/--suffix_length: Suffix length when using + the prefix_suffix otu picker [default: 50]" name="suffix_length" + optional="True" type="integer"/> + <param default="union" label="-F/--non_chimeras_retention: Selects subsets + of sequences detected as non-chimeras to retain after de novo and + reference based chimera detection. Options are intersection or union. + union will retain sequences that are flagged as non-chimeric from either + filter, while intersection will retain only those sequences that are + flagged as non-chimeras from both detection methods. [default: union]" + name="non_chimeras_retention" optional="True" type="text"/> + </inputs> + <outputs> + <data format="txt" from_work_dir="fastasplit/*_otus.txt" + name="pick_otus.txt" label="pick_otus.txt"/> + <data format="txt" from_work_dir="fastasplit/*_otus.log" + name="pick_otus.log" label="pick_otus.log"/> + <data format="txt" from_work_dir="fastasplit/*_failures.txt" + name="pick_otus_failures.txt" label="pick_otus_failures.txt"/> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ +**What it does** + +The OTU picking step assigns similar sequences to operational taxonomic units, or OTUs, by clustering sequences based on a user-defined similarity threshold. Sequences which are similar at or above the threshold level are taken to represent the presence of a taxonomic unit (e.g., a genus, when the similarity threshold is set at 0.94) in the sequence collection. + +Currently, the following clustering methods have been implemented in QIIME: + +1. uclust, creates "seeds" of sequences which generate clusters based on percent identity. + +2. uclust_ref, as uclust, but takes a reference database to use as seeds. New clusters can be toggled on or off. + +3. usearch, creates "seeds" of sequences which generate clusters based on percent identity, filters low abundance clusters, performs de novo and reference based chimera detection. + +4. usearch_ref, as usearch, but takes a reference database to use as seeds. New clusters can be toggled on or off. + +5. sumaclust, creates "seeds" of sequences which generate clusters based on similarity threshold. + +6. swarm, creates "seeds" of sequences which generate clusters based on a resolution threshold. + + +Chimera checking with usearch 6.X is implemented in identify_chimeric_seqs.py. Chimera checking should be done first with usearch 6.X, and the filtered resulting fasta file can then be clustered. + + +The primary inputs for pick_otus.py are: + +1. A FASTA file containing sequences to be clustered + +2. An OTU threshold (default is 0.97, roughly corresponding to species-level OTUs); + +3. The method to be applied for clustering sequences into OTUs. + +pick_otus.py takes a standard fasta file as input. + + +The output consists of two files (i.e. seqs_otus.txt and seqs_otus.log). The .txt file is composed of tab-delimited lines, where the first field on each line corresponds to an (arbitrary) cluster identifier, and the remaining fields correspond to sequence identifiers assigned to that cluster. Sequence identifiers correspond to those provided in the input FASTA file. Usearch (i.e. usearch quality filter) can additionally have log files for each intermediate call to usearch. + +Example lines from the resulting .txt file: + += ==== ==== ==== +0 seq1 seq5 +1 seq2 +2 seq3 +3 seq4 seq6 seq7 += ==== ==== ==== + +This result implies that four clusters were created based on 7 input sequences. +The first cluster (cluster id 0) contains two sequences, sequence ids seq1 and +seq5; the second cluster (cluster id 1) contains one sequence, sequence id seq2; +the third cluster (cluster id 2) contains one sequence, sequence id seq3, and the +final cluster (cluster id 3) contains three sequences, sequence ids seq4, seq6, +and seq7. + +The resulting .log file contains a list of parameters passed to the pick_otus.py +script along with the output location of the resulting .txt file.</help> + ]]> + </help> + + <citations> + <expand macro="citations" /> + <citation type="doi">10.1093/bioinformatics/btv231</citation> + <citation type="doi">10.1093/bioinformatics/btq461</citation> + <citation type="doi">10.1093/bioinformatics/bts611</citation> + <citation type="doi">10.7287/peerj.preprints.386v1/supp-1</citation> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/pick_rep_set.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,64 @@ +<tool id="qiime_pick_rep_set" name="pick rep set" version="1.9.1galaxy1"> + <description>Pick representative set of sequences</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <command> +<![CDATA[ + pick_rep_set.py + -i $input_file + -m $rep_set_picking_method + -s $sort_by + #if str($fasta_file) != 'None': + -f $fasta_file + #end if + -o $result_fp -l rep_set.log + + #if str($reference_seqs_fp) != 'None': + -r $reference_seqs_fp + #end if +]]> + </command> + + <inputs> + <param label="-i/--input_file: Path to input otu mapping file [REQUIRED]" name="input_file" optional="False" type="data"/> + <param label="-f/--fasta_file: Path to input fasta file [REQUIRED if not picking against a reference set; default: None]" name="fasta_file" optional="True" type="data"/> + <param label="-m/--rep_set_picking_method: Method for picking representative sets. Valid choices are random, longest, most_abundant, first [default: first (first chooses cluster seed when picking otus with uclust)]" name="rep_set_picking_method" optional="True" type="select"> + <option value="random">random</option> + <option value="longest">longest</option> + <option value="most_abundant">most_abundant</option> + <option value="first">first</option> + </param> + <param label="-s/--sort_by: sort by otu or seq_id [default: otu]" name="sort_by" optional="True" type="select"> + <option value="otu">otu</option> + <option value="seq_id">seq_id</option> + </param> + <param label="-r/--reference_seqs_fp: collection of preferred representative sequences [default: None]" name="reference_seqs_fp" optional="True" type="data"/> + </inputs> + + <outputs> + <data format="fasta" name="result_fp" label="rep_set_seqs.fasta"/> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ +**How it does?** + +After picking OTUs, you can then pick a representative set of sequences. For each OTU, you will end up with one sequence that can be used in subsequent analyses. + +The output from pick_rep_set.py is a single FASTA file containing one sequence per OTU. The FASTA header lines will be the OTU identifier (from here on used as the unique sequence identifier) followed by a space, followed by the sequence identifier originally associated with the representative sequence. The name of the output FASTA file will be <input_sequences_filepath>_rep_set.fasta by default, or can be specified via the "-o" parameter. +]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/plot_taxa_summary.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,194 @@ +<tool id="qiime_plot_taxa_summary" name="Make taxonomy summary charts" version="1.9.1"> + <description>based on taxonomy assignment</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <version_command><![CDATA[ + plot_taxa_summary.py --version + ]]> + </version_command> + + <command> +<![CDATA[ + #set $chosen_chart_type = str( $chart_type ).split( "," ) + + plot_taxa_summary.py + -i $counts_fname + + -o taxa_plots + + #if str($labels): + -l $labels + #end if + + -n $num_categories + + #if str( $colorby) + -b $colorby + #end if + + -k $background_color + -d $dpi + -x $x_width + -y $y_height + -w $bar_width + + -c $chart_type + $include_html_legend + -a $label_type + + #if "area" in $chosen_chart_type: + && + mkdir -p $area_charts_html_report.files_path + && + cp -r taxa_plots/area_charts.html $area_charts_html_report.files_path + && + cp -r taxa_plots/charts $area_charts_html_report.files_path + && + cp -r taxa_plots/css $area_charts_html_report.files_path + && + cp -r taxa_plots/js $area_charts_html_report.files_path + && + mv $area_charts_html_report.files_path/area_charts.html $area_charts_html_report + #end if + + #if "bar" in $chosen_chart_type: + && + mkdir -p $bar_charts_html_report.files_path + && + cp -r taxa_plots/bar_charts.html $bar_charts_html_report.files_path + && + cp -r taxa_plots/charts $bar_charts_html_report.files_path + && + cp -r taxa_plots/css $bar_charts_html_report.files_path + && + cp -r taxa_plots/js $bar_charts_html_report.files_path + && + mv $bar_charts_html_report.files_path/bar_charts.html $bar_charts_html_report + #end if + + #if "pie" in $chosen_chart_type: + && + mkdir -p $pie_charts_html_report.files_path + && + cp -r taxa_plots/pie_charts.html $pie_charts_html_report.files_path + && + cp -r taxa_plots/charts $pie_charts_html_report.files_path + && + cp -r taxa_plots/css $pie_charts_html_report.files_path + && + cp -r taxa_plots/js $pie_charts_html_report.files_path + && + mv $pie_charts_html_report.files_path/pie_charts.html $pie_charts_html_report + #end if +]]> + </command> + + <inputs> + <param name="counts_fname" type="data" format="tabular,txt,tsv" + label="Summarized taxa files" multiple="True" + help="(-i/--counts_fname)"/> + + <param name="labels" type="text" value="" label="Comma-separated list of + taxonomic levels (optional)" help="(-l/--labels)" optional="True"/> + + <param name="num_categories" type="integer" value="20" label="Maximum + number of taxonomies to show in each pie chart" help="All additional + taxonomies are grouped into an 'other' category. This functionality + only applies to the pie charts(-n/--num_categories)"/> + + <param name="colorby" type="text" value="" label="Categories to color + by in the plots from the metadata mapping file (optional)" + help="The categories must match the name of a column header in the + mapping file exactly and multiple categories can be list by comma + separating them without spaces(-b/--colorby)" optional="True"/> + + <param name="background_color" type="select" label="Background color to + use in the plots" help="(-k/--background_color)"> + <option value="white">White</option> + <option value="black">Black</option> + </param> + + <param name="dpi" type="integer" value="80" label="Resolution of the plot" + help="(-d/--dpi)"/> + + <param name="x_width" type="integer" value="12" label="Width of the + x-axis to use in the plots" help="(-x/--x_width)"/> + + <param name="y_height" type="integer" value="12" label="Height of the + y-axis to use in the plots" help="(-y/--y_height)"/> + + <param name="bar_width" type="float" value="0.75" min="0" maw="1" + label="Width of the bars in the bar graph" help="(-w/--bar_width)"/> + + <param name="chart_type" type="select" label="Type of chart to plot" + help="(-c/--chart_type)" multiple="True" display="checkboxes"> + <option value="area" selected="true">Area</option> + <option value="bar" selected="true">Bar</option> + <option value="pie">Pie</option> + </param> + + <param name="include_html_legend" type="boolean" label="Include HTML + legend?" truevalue="--include_html_legend" falsevalue="" checked="False" + help="If checked, the writing of the legend in the html page is + included (-s/--include_html_legend)" /> + + <param name="label_type" type="select" label="Label type" + help="If the label type is defined as numeric, the x-axis will be + scaled accordingly. Otherwise the x-values will treated categorically + and be evenly spaced(-a/--label_type)"> + <option value="categorical" selected="true">Categorical</option> + <option value="numeric">Numeric</option> + </param> + </inputs> + + <outputs> + <data name="bar_charts_html_report" format="html" + label="${tool.name} on ${on_string}: Bar charts"> + <filter>'bar' in chart_type</filter> + </data> + <data name="pie_charts_html_report" format="html" + label="${tool.name} on ${on_string}: Pie charts"> + <filter>'pie' in chart_type</filter> + </data> + <data name="area_charts_html_report" format="html" + label="${tool.name} on ${on_string}: Area charts"> + <filter>'area' in chart_type</filter> + </data> + </outputs> + + <tests> + <test> + <param name="counts_fname" + value="summarize_taxa_L2.tab"/> + <param name="num_categories" value="20"/> + <param name="background_color" value="white"/> + <param name="dpi" value="80" /> + <param name="x_width" value="12" /> + <param name="y_height" value="12" /> + <param name="bar_width" value="0.75" /> + <param name="chart_type" value="area,bar"/> + <param name="include_html_legend" value="" /> + <param name="label_type" value="categorical"/> + </test> + </tests> + + <help><![CDATA[ + +**What it does** + +This script automates the construction of pie, bar and area charts showing the breakdown of taxonomy by given levels. + +More information about this tool is available on +`QIIME documentation <http://qiime.org/scripts/plot_taxa_summary.html>`_. + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/split_libraries.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,285 @@ +<tool id="qiime_split_libraries" name="Split libraries" version="1.9.1"> + <description>according to barcodes specified in mapping file</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <version_command><![CDATA[ + split_libraries.py --version + ]]></version_command> + + <command><![CDATA[ + split_libraries.py + -m $mapping_fp + -o split_libraries + + #set $seq_files = '' + #set $sep = '' + #for $file in $input_files_fasta + #set $seq_files += $sep + str($file) + #set $sep = ',' + #end for + -f $seq_files + + #if str($input_files_qual) != 'None': + set $files = '' + #set $sep = '' + #for $file in $input_files_qual + #set $files += $sep + str($file) + #set $sep = ',' + #end for + -q $files + #end if + + -l $min_seq_length + -L $max_seq_length + $trim_seq_length + + $keep_primer + $keep_barcode + + -a $max_ambig + -H $max_homopolymer + -M $max_primer_mismatch + + #if str( $barcode_type.barcode_selector ) != "custom_length" + -b $barcode_type.barcode_selector + #else + -b $barcode_type.barcode_length + #end if + + -e $max_barcode_errors + -n $start_numbering_at + $retain_unassigned_reads + $disable_bc_correction + + #if str($input_files_qual) != 'None': + -s $min_qual_score + -w $qual_score_window + $discard_bad_windows + $record_qual_scores + #end if + + $disable_primers + + $reverse_primers.reverse_primers_test + #if str($reverse_primers.reverse_primers_test) == '--reverse_primers': + --reverse_primer_mismatches $reverse_primers.reverse_primer_mismatches + #end if + + #if str($median_length_filtering): + -i $median_length_filtering + #end if + + #if str($added_demultiplex_field): + -j $added_demultiplex_field + #end if +]]> + </command> + + <inputs> + <param name="mapping_fp" label="Metadata mapping filepath" type="data" + format="tabular,txt,tsv,csv" help="The file must contain header + line indicating SampleID in the first column and BarcodeSequence in + the second, LinkerPrimerSequence in the third. It is recommended to + check the mapping file using the dedicated file (-m/--mapping_fp)"/> + + <param name="input_files_fasta" type="data" format="fasta" + label="Input fasta files" multiple="True" help="(-f/--fasta)"/> + + <param name="input_files_qual" type="data" + format="qual,qual454,qualillumina,qualsolexa,qualsolid" + label="Input quality files (optional)" multiple="True" + help="(-q/--qual)" optional="True"/> + + <param name="min_seq_length" type="integer" value="200" + label="Minimum sequence length" help="(-l/--min_seq_length)"/> + + <param name="max_seq_length" type="integer" value="1000" + label="Maximum sequence length" help="(-L/--max_seq_length)"/> + + <param name="trim_seq_length" type="boolean" label="Compute sequence + lengths after trimming and barcodes?" truevalue="-t" falsevalue="" + selected="False" help="(-t/--trim_seq_length)" /> + + <param name="min_qual_score" type="integer" value="25" + label="Minimum average quality score allowed in read" + help="(-s/--min_qual_score)"/> + + <param name="keep_primer" type="boolean" label="Remove primer from + sequences?" truevalue="" falsevalue="--keep_primer" + selected="True" help="(-k/--keep_primer)" /> + + <param name="keep_barcode" type="boolean" label="Remove barcode from + sequences?" truevalue="" falsevalue="--keep_barcode" + selected="True" help="(-B/--keep_barcode)" /> + + <param name="max_ambig" type="integer" value="6" + label="Maximum number of ambiguous bases" help="(-a/--max_ambig)"/> + + <param name="max_homopolymer" type="integer" value="6" + label="Maximum length of homopolymer run" help="(-H/--max_homopolymer)"/> + + <param name="max_primer_mismatch" type="integer" value="0" + label="Maximum number of primer mismatch" help="(-M/--max_primer_mismatch)"/> + + <conditional name="barcode_type"> + <param name="barcode_selector" type="select" label="Type of barcode" + help="(-b/ --barcode_type)"> + <option value="hamming_8">hamming_8</option> + <option value="golay_12" selected="true">golay_12</option> + <option value="variable_length">variable_length (disable any barcode correction)</option> + <option value="custom_length">Custom length</option> + </param> + <when value="hamming_8" /> + <when value="golay_12" /> + <when value="variable_length" /> + <when value="custom_length"> + <param name="barcode_length" type="integer" value="4" + label="Barcode length"/> + </when> + </conditional> + + <param name="max_barcode_errors" type="float" value="1.5" + label="Maximum number of errors in barcode" + help="(-e/--max_barcode_errors)"/> + + <param name="start_numbering_at" type="integer" value="1" + label="Sequence id to use for the first seuqence" + help="(-n/--start_numbering_at)"/> + + <param name="retain_unassigned_reads" type="boolean" label="Retain + sequences with are Unassigned in the output sequence file?" + truevalue="--retain_unassigned_reads" falsevalue="" + selected="False" help="(--retain_unassigned_reads)" /> + + <param name="retain_unassigned_reads" type="boolean" label="Retain + sequences with are Unassigned in the output sequence file?" + truevalue="--retain_unassigned_reads" falsevalue="" + selected="False" help="(--retain_unassigned_reads)" /> + + <param name="disable_bc_correction" type="boolean" label="Disable attempts + to find nearest corrected barcode?" + truevalue="(--disable_bc_correction)" falsevalue="" + selected="False" help="It can improve performance. + (-c/--disable_bc_correction)" /> + + <param name="qual_score_window" type="integer" value="0" + label="Size of the sliding window" help="If the average score of a + continuous set of w nucleotides falls below the threshold, the sequence + is discarded. A good value would be 50. 0 (zero) means no filtering. + Must pass a .qual file (see -q parameter) if this functionality is + enabled. Default behavior for this function is to truncate the sequence + at the beginning of the poor quality window, and test for minimal + length (-l parameter) of the resulting sequence (-w/--qual_score_window)"/> + + <param name="discard_bad_windows" type="boolean" label="Discard any + sequences where a bad window is found?" + truevalue="--discard_bad_windows" falsevalue="" + selected="False" help="It will work if the sliding window length is bigger + than 0 (-g/--discard_bad_windows)" /> + + <param name="disable_primers" type="boolean" label="Disable primer usage + when demultiplexing?" truevalue="--disable_primers" falsevalue="" + selected="False" help="It should be enabled for unusual circumstances, + such as analyzing Sanger sequence data generated with different primers + (-p/--disable_primers)" /> + + <conditional name="reverse_primers"> + <param name="reverse_primers_test" type="select" label="Enable removal + of the reverse primer and any subsequence sequence from the end + of each read?" help="(-z/--reverse_primers)" > + <option value="--reverse_primers">Yes</option> + <option value="" selected="true">No</option> + </param> + <when value="" /> + <when value="--reverse_primers" > + <param name="reverse_primer_mismatches" type="integer" value="0" + label="Number of allowed mismatches for reverse primers" + help="(--reverse_primer_mismatches)"/> + </when> + </conditional> + + <param name="record_qual_scores" type="boolean" label="Record quality + scores for all sequences that are recorded?" truevalue="--record_qual_scores" + falsevalue="" selected="False" help="If this option is enabled, a file + named seqs_filtered.qual will be created in the output directory, and + will contain the same sequence IDs in the seqs.fna file and sequence + quality scores matching the bases present in the seqs.fna file + (-d/--record_qual_scores)" /> + + <param name="median_length_filtering" type="integer" + label="Median length filtering (optional)" help="It disables minimum + and maximum sequence length filtering, and instead calculates the median + sequence length and filters the sequences based upon the number of median + absolute deviations specified by this parameter. Any sequences with + lengths outside the number of deviations will be removed + (-i/--median_length_filtering)" + optional="True"/> + + <param name="added_demultiplex_field" type="text" label="Field + to use in the mapping file as additional demultiplexing (optional)" + help="It can be used with or without barcodes. All combinations of + barcodes/primers and these fields must be unique. The fields must contain + values that can be parsed from the fasta labels such as 'plate=R_2008_12_09'. + In this case, 'plate' would be the column header and 'R_2008_12_09' + would be the field data (minus quotes) in the mapping file. + To use the run prefix from the fasta label, such as 'FLP3FBN01ELBSX', + where 'FLP3FBN01' is generated from the run ID, use 'run_prefix' and + set the run prefix to be used as the data under the column header + 'run_prefix' (-j/--added_demultiplex_field)" optional="True"/> + + <param name="truncate_ambi_bases" type="boolean" label="Enable to truncate + at the first N character encountered in the sequences?" + truevalue="--truncate_ambi_bases" falsevalue="" selected="False" + help="This will disable testing for ambiguous bases + (-x/--truncate_ambi_bases)"/> + </inputs> + + <outputs> + <data name="sequences" format="fasta" + from_work_dir="split_libraries/*.fna" + label="${tool.name} on ${on_string}: sequences"/> + + <data name="log" format="txt" + from_work_dir="split_libraries/split_library_log.txt" + label="${tool.name} on ${on_string}: log"/> + + <data name="histograms" format="txt" + from_work_dir="split_libraries/histograms.txt" + label="${tool.name} on ${on_string}: histograms"/> + + <data name="quality" format="qual,qual454,qualillumina,qualsolexa,qualsolid" + from_work_dir="split_libraries/*.qual" + label="${tool.name} on ${on_string}: quality"> + <filter>record_qual_scores is True</filter> + </data> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ + +**What it does** + +This tool splits libraries according to barcodes specified in mapping file. + +Since newer sequencing technologies provide many reads per run (e.g. the 454 GS FLX Titanium series can produce 400-600 million base pairs with 400-500 base pair read lengths) researchers are now finding it useful to combine multiple samples into a single 454 run. This multiplexing is achieved through the application of a pyrosequencing-tailored nucleotide barcode design (described in (Parameswaran et al., 2007)). By assigning individual, unique sample specific barcodes, multiple sequencing runs may be performed in parallel and the resulting reads can later be binned according to sample. The script %prog performs this task, in addition to several quality filtering steps including user defined cut-offs for: sequence lengths; end-trimming; minimum quality score. To summarize, by using the fasta, mapping, and quality files, the program %prog will parse sequences that meet user defined quality thresholds and then rename each read with the appropriate Sample ID, thus formatting the sequence data for downstream analysis. If a combination of different sequencing technologies are used in any particular study, %prog can be used to perform the quality-filtering for each library individually and the output may then be combined. + +Sequences from samples that are not found in the mapping file (no corresponding barcode) and sequences without the correct primer sequence will be excluded. Additional scripts can be used to exclude sequences that match a given reference sequence (e.g. the human genome; exclude_seqs_by_blast.py) and/or sequences that are flagged as chimeras (identify_chimeric_seqs.py). + +More information about this tool is available on +`QIIME documentation <http://qiime.org/scripts/split_libraries.html>`_. + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/split_libraries_fastq.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,239 @@ +<tool id="split_libraries_fastq" name="Split fastq libraries" version="1.9.1"> + <description>to performs demultiplexing of Fastq sequence data</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <version_command><![CDATA[ + split_libraries_fastq.py --version + ]]> + </version_command> + + <command><![CDATA[ + split_libraries_fastq.py + + #set $seq_files = '' + #set $sep = '' + #for $file in $input_fastq_files + #set $seq_files += $sep + str($file) + #set $sep = ',' + #end for + -i $seq_files + + -o split_libraries + + #set $mapping_files = '' + #set $sep = '' + #for $file in $input_mapping_files + #set $mapping_files += $sep + str($file) + #set $sep = ',' + #end for + -m $mapping_files + + #set $barcode_files = '' + #set $sep = '' + #for $file in $input_files_barcode_read_fps + #set $barcode_files += $sep + str($file) + #set $sep = ',' + #end for + -b $barcode_files + + $store_qual_scores + + #if str($sample_ids): + --sample_ids $sample_ids + #end if + + $store_demultiplexed_fastq + $retain_unassigned_reads + -r $max_bad_run_length + -p $min_per_read_length_fraction + -n $sequence_max_n + -s $start_seq_id + + $rev_comp_barcode + $rev_comp_mapping_barcodes + $rev_comp + + -q $phred_quality_threshold + + #if str( $barcode_type.barcode_selector ) != "custom_length" + --barcode_type $barcode_type.barcode_selector + #else + --barcode_type $barcode_type.barcode_length + #end if + + --max_barcode_errors $max_barcode_errors + + $phred_offset +]]> + </command> + + <inputs> + <param name="input_fastq_files" type="data" + format="fastq,fastqsanger,fastqsolexa" + label="Input fastq files" multiple="True" help="(-i/--sequence_read_fps)"/> + + <param name="input_mapping_files" type="data" + format="txt,tabular,tsv,csv" label="Metadata mapping files (optional)" + multiple="True" optional="True" help="(-m/--mapping_fps)"/> + + <param name="input_files_barcode_read_fps" type="data" + format="fastq,fastqsanger,fastqsolexa" label="Barcode read files (optional)" + multiple="True" optional="True" help="(-b/--barcode_read_fps)"/> + + <param name="store_qual_scores" type="boolean" label="Store quality strings + in files?" truevalue="--store_qual_scores" falsevalue="" checked="False" + help="(--store_qual_scores)" /> + + <param name="sample_ids" type="text" label="Comma-separated list of samples + ids to be applied to all sequences (optional)" optional="True" + help="It must be one per input file path (used when data is not + multiplexed, --sample_ids)"/> + + <param name="store_demultiplexed_fastq" type="boolean" label="Write + demultiplexed fastq files?" truevalue="--store_demultiplexed_fastq" + falsevalue="" checked="False" help="(--store_demultiplexed_fastq)" /> + + <param name="retain_unassigned_reads" type="boolean" label="Retain + sequences which don’t map to a barcode in the mapping file?" + truevalue="--retain_unassigned_reads" falsevalue="" checked="False" + help="Sample ID will be 'Unassigned' (--retain_unassigned_reads)" /> + + <param name="max_bad_run_length" type="integer" value="3" + label="Maximum number of consecutive low quality base calls allowed + before truncating a read" help="(-r/--max_bad_run_length)" /> + + <param name="min_per_read_length_fraction" type="float" value="0.75" + label="Minimum number of consecutive high quality base calls to + include a read (per single end read) as a fraction of the input read + length" help="(-p/--min_per_read_length_fraction)" /> + + <param name="sequence_max_n" type="integer" value="0" + label="Maximum number of N characters allowed in a sequence to retain + it" help="This is applied after quality trimming, and is total over + combined paired end reads if applicable (-n/--sequence_max_n)" /> + + <param name="start_seq_id" type="integer" value="0" + label="Start seq_ids as ascending integers beginning with start_seq_id" + help="(-s/--start_seq_id)" /> + + <param name="rev_comp_barcode" type="boolean" label="Reverse complement + barcode reads before lookup?" truevalue="--rev_comp_barcode" + falsevalue="" checked="False" help="(--rev_comp_barcode)" /> + + <param name="rev_comp_mapping_barcodes" type="boolean" label="Reverse + complement barcode in mapping before lookup?" + truevalue="--rev_comp_mapping_barcodes" falsevalue="" checked="False" + help="It is useful if barcodes in mapping file are reverse + complements of golay codes (--rev_comp_mapping_barcodes)" /> + + <param name="rev_comp" type="boolean" label="Reverse omplement sequence + before writing to output file?" truevalue="--rev_comp" falsevalue="" + checked="False" help="(--rev_comp)" /> + + <param name="phred_quality_threshold" type="integer" value="3" + label="Maximum unacceptable Phred quality score" help="E.g., for + Q20 and better, 19 must be specified (-q/--phred_quality_threshold)" /> + + <conditional name="barcode_type"> + <param name="barcode_selector" type="select" label="Type of barcode" + help="(--barcode_type)"> + <option value="hamming_8">hamming_8</option> + <option value="golay_12" selected="true">golay_12</option> + <option value="variable_length">variable_length (disable any barcode correction)</option> + <option value="custom_length">Custom length</option> + <option value="not-barcoded">Data not barcoded</option> + </param> + <when value="hamming_8" /> + <when value="golay_12" /> + <when value="variable_length" /> + <when value="custom_length"> + <param name="barcode_length" type="integer" value="4" + label="Barcode length"/> + </when> + <when value="not-barcoded" /> + </conditional> + + <param name="max_barcode_errors" type="float" value="1.5" + label="Maximum number of errors in barcode" + help="(--max_barcode_errors)" /> + + <param name="phred_offset" type="select" label="Ascii offset to use when + decoding phred scores" help="(--phred_offset)"> + <option value="--phred_offset 33">33</option> + <option value="--phred_offset 64">64</option> + <option value="" selected="true">Automatically determined</option> + </param> + </inputs> + + <outputs> + <data name="log" format="txt" + from_work_dir="split_libraries/split_library_log.txt" + label="${tool.name} on ${on_string}: log"/> + <data name="histograms" format="tabular" + from_work_dir="split_libraries/histograms.txt" + label="${tool.name} on ${on_string}: histograms"/> + <data name="seqs" format="fasta" + from_work_dir="split_libraries/seqs.fna" + label="${tool.name} on ${on_string}: sequences"/> + <data name="seqs_qual" format="qual" + from_work_dir="split_libraries/seqs.qual" + label="${tool.name} on ${on_string}: sequence qualities"> + <filter>store_qual_scores is True</filter> + </data> + <data name="seqs_fastq" format="fastq" + from_work_dir="split_libraries/seqs.fastq" + label="${tool.name} on ${on_string}: demultiplexed sequences (fastq)"> + <filter>store_demultiplexed_fastq is True</filter> + </data> + </outputs> + + <tests> + <test> + <param name="input_fastq_files" value="forward_reads.fastq"/> + <param name="input_mapping_files" value="map.tsv"/> + <param name="input_files_barcode_read_fps" value="barcodes.fastq"/> + <param name="store_qual_scores" value="--store_qual_scores" /> + <param name="store_demultiplexed_fastq" value="--store_demultiplexed_fastq" /> + <param name="retain_unassigned_reads" value=""/> + <param name="max_bad_run_length" value="3"/> + <param name="min_per_read_length_fraction" value="0.75"/> + <param name="sequence_max_n" value="0"/> + <param name="start_seq_id" value="0" /> + <param name="rev_comp_barcode" value="" /> + <param name="rev_comp_mapping_barcodes" value="" /> + <param name="rev_comp" value="" /> + <param name="start_seq_id" value="3"/> + <param name="barcode_selector" value="golay_12"/> + <param name="max_barcode_errors" value="1.5"/> + <param name="phred_offset" value="" /> + + <output name="log" file="split_fastq_libraries_log.txt"/> + <output name="seqs" file="split_fastq_libraries_sequences.fasta"/> + <output name="histograms" file="split_fastq_libraries_histograms.tabular"/> + <output name="histograms" file="split_fastq_libraries_histograms.tabular"/> + <output name="seqs_qual" file="split_fastq_libraries_sequence_qualities.qual"/> + <output name="seqs_fastq" file="split_fastq_libraries_demultiplexed_sequences.fastq"/> + </test> + </tests> + + <help><![CDATA[ + +**What it does** + +This tool performs demultiplexing of Fastq sequence data where barcodes and sequences are contained in two separate fastq files (common on Illumina runs). + +More information about this tool is available on +`QIIME documentation <http://qiime.org/scripts/split_libraries_fastq.html>`_. +]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> + +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/summarize_taxa.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,186 @@ +<tool id="qiime_summarize_taxa" name="Summarize taxa" version="1.9.1"> + <description>and store results in a new table or appended to an existing + mapping file + </description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <version_command><![CDATA[ + summarize_taxa.py --version + ]]> + </version_command> + + <command> +<![CDATA[ + summarize_taxa.py + -i $otu_table_fp + -o summarize_taxa + + -L $level + + #if str($mapping) != 'None': + -m $mapping + #end if + + --md_identifier $md_identifier + + $md_as_string + + $absolute_abundance + + #if str( $lower_percentage ) + -l $lower_percentage + #end if + + #if str( $upper_percentage) + -u $upper_percentage + #end if + + $transposed_output + $suppress_classic_table_output + $suppress_biom_table_output +]]> + </command> + + <inputs> + <param name="otu_table_fp" type="data" format="tabular,tsv,txt,biom" + label="Input OTU table" help="(-i/--otu_table_fp)"/> + + <param name="level" type="select" label="Taxonomic level to summarize by" + help="(-L/--level)" multiple="True" display="checkboxes"> + <option value="1" >Kingdom</option> + <option value="2" selected="true">Phylum</option> + <option value="3" selected="true">Class</option> + <option value="4" selected="true">Order</option> + <option value="5" selected="true">Family</option> + <option value="6" selected="true">Genus</option> + <option value="7" >Species</option> + </param> + + <param name="mapping" type="data" format="tabular,tsv,csv" + label="Input metadata mapping (optional)" help="If supplied, then the + taxon information will be added to this file. This option is useful for + coloring PCoA plots by taxon abundance or to perform statistical + tests of taxon/mapping associations (-m/--mapping)" optional="True"/> + + <param name="md_identifier" type="text" value="taxonomy" label="Relevant + observation metadata key" help="(--md_identifier)" /> + + <param name="md_as_string" type="boolean" label="Include metadata as string?" + truevalue="--md_as_string" falsevalue="" checked="False" + help="By default, metadata is included as list (--md_as_string)" /> + + <param name="absolute_abundance" type="boolean" label="Report absolute + abundance?" truevalue="--absolute_abundance" falsevalue="" checked="False" + help="By default, relative abundance is reported (--absolute_abundance)" /> + + <param name="lower_percentage" type="float" label="Percentage threshold to + remove highly present OTU (optional)" help="If present, OTUs having + higher absolute abundance are trimmed. To remove OTUs that make up + more than 5% of the total dataset you would pass 0.05 (-l/--lower_percentage)" + optional="True"/> + + <param name="upper_percentage" type="float" label="Percentage threshold to + remove lowly present OTU (optional)" help="If present, OTUs having + lower absolute abundance are trimmed. To remove the OTUs that + makes up less than 45% of the total dataset you would pass 0.45 + (-u/--upper_percentage)" optional="True"/> + + <param name="transposed_output" type="boolean" label="Transpose output?" + truevalue="--transposed_output" falsevalue="" checked="False" + help="If checked, the output will be written transposed from the + regular output. This is helpful in cases when you want to use Site + Painter to visualize your data (-t/--transposed_output)" /> + + <param name="suppress_classic_table_output" type="boolean" + label="Suppress creation of the classic (TSV) format taxon table?" + truevalue="--suppress_classic_table_output" falsevalue="" checked="False" + help="This option is ignored if a mapping file is present + (--suppress_classic_table_output)" /> + + <param name="suppress_biom_table_output" type="boolean" + label="Suppress creation of the BIOM-formatted taxon table?" + truevalue="--suppress_biom_table_output" falsevalue="" checked="False" + help="This option is ignored if a mapping file is present + (--suppress_biom_table_output)" /> + </inputs> + + <outputs> + <data name="L1_tab" format="tabular" from_work_dir="summarize_taxa/*_L1.txt" + label="${tool.name} on ${on_string}: L1 tab"> + <filter>"1" in level and suppress_classic_table_output is False</filter> + </data> + + <data name="L2_tab" format="tabular" from_work_dir="summarize_taxa/*_L2.txt" + label="${tool.name} on ${on_string}: L2 tab"> + <filter>"2" in level and suppress_classic_table_output is False</filter> + </data> + + <data name="L3_tab" format="tabular" from_work_dir="summarize_taxa/*_L3.txt" + label="${tool.name} on ${on_string}: L3 tab"> + <filter>"3" in level and suppress_classic_table_output is False</filter> + </data> + + <data name="L4_tab" format="tabular" from_work_dir="summarize_taxa/*_L4.txt" + label="${tool.name} on ${on_string}: L4 tab"> + <filter>"4" in level and suppress_classic_table_output is False</filter> + </data> + + <data name="L5_tab" format="tabular" from_work_dir="summarize_taxa/*_L5.txt" + label="${tool.name} on ${on_string}: L5 tab"> + <filter>"5" in level and suppress_classic_table_output is False</filter> + </data> + + <data name="L6_tab" format="tabular" from_work_dir="summarize_taxa/*_L6.txt" + label="${tool.name} on ${on_string}: L6 tab"> + <filter>"6" in level and suppress_classic_table_output is False</filter> + </data> + + <data name="L7_tab" format="tabular" from_work_dir="summarize_taxa/*_L7.txt" + label="${tool.name} on ${on_string}: L7 tab"> + <filter>"7" in level and suppress_classic_table_output is False</filter> + </data> + </outputs> + + <tests> + <test> + <param name="otu_table_fp" + value="pick_open_reference_otus_otu_table_with_taxo.biom"/> + <param name="level" value="2,3,4,5,6" /> + <param name="mapping" + value="validate_mapping_file_corrected_map.tabular"/> + <param name="md_identifier" value="taxonomy"/> + <param name="md_as_string" value=""/> + <param name="delimiter" value=";" /> + <param name="absolute_abundance" value=""/> + <param name="transposed_output" value=""/> + <param name="suppress_classic_table_output" value="" /> + <param name="suppress_biom_table_output" value="" /> + + <output name="L2_tab" value="summarize_taxa_L2.tabular" /> + <output name="L3_tab" value="summarize_taxa_L3.tabular" /> + <output name="L4_tab" value="summarize_taxa_L4.tabular" /> + <output name="L5_tab" value="summarize_taxa_L5.tabular" /> + <output name="L6_tab" value="summarize_taxa_L6.tabular" /> + </test> + </tests> + + <help><![CDATA[ + +**What it does** + +This tool provides summary information of the representation of taxonomic groups within each sample. It takes an OTU table that contains taxonomic information as input. + +More information about this tool is available on +`QIIME documentation <http://qiime.org/scripts/summarize_taxa.html>`_. + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/summarize_taxa_through_plots.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,140 @@ +<tool id="qiime_summarize_taxa_through_plots" name="Perform taxonomy" version="1.9.1"> + <description>summaries and plots</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <version_command><![CDATA[ + summarize_taxa_through_plots.py --version + ]]> + </version_command> + + <command> +<![CDATA[ + summarize_taxa_through_plots.py + -i $otu_table_fp + -o summarize_taxa_through_plots + + #if $mapping_fp + -m $mapping_fp + #end if + + #if $mapping_category + -c $mapping_category + #end if + + $sort + + && + + mkdir -p $area_charts_html_report.files_path + && + cp -r summarize_taxa_through_plots/taxa_summary_plots/area_charts.html $area_charts_html_report.files_path + && + cp -r summarize_taxa_through_plots/taxa_summary_plots/charts $area_charts_html_report.files_path + && + cp -r summarize_taxa_through_plots/taxa_summary_plots/css $area_charts_html_report.files_path + && + cp -r summarize_taxa_through_plots/taxa_summary_plots/js $area_charts_html_report.files_path + && + mv $area_charts_html_report.files_path/area_charts.html $area_charts_html_report + + && + mkdir -p $bar_charts_html_report.files_path + && + cp -r summarize_taxa_through_plots/taxa_summary_plots/bar_charts.html $bar_charts_html_report.files_path + && + cp -r summarize_taxa_through_plots/taxa_summary_plots/charts $bar_charts_html_report.files_path + && + cp -r summarize_taxa_through_plots/taxa_summary_plots/css $bar_charts_html_report.files_path + && + cp -r summarize_taxa_through_plots/taxa_summary_plots/js $bar_charts_html_report.files_path + && + mv $bar_charts_html_report.files_path/bar_charts.html $bar_charts_html_report + +]]> + </command> + + <inputs> + <param name="otu_table_fp" type="data" format="biom,tabular,txt,tsv" + label="Input OTU table" help="(-i/--otu_table_fp)"/> + + <param name="mapping_fp" type="data" format="tabular,tsv,csv" + label="Input metadata mapping (optional)" help="(-m, --mapping_fp)" + optional="True"/> + + <param name="mapping_category" type="text" value="" label="Category to + use to summarize OTU table" help="(-c/--mapping_category)" + optional="True"/> + + <param name="sort" type="boolean" label="Sort OTU table?" + truevalue="--sort" falsevalue="" selected="False" + help="(-s/--sort)" /> + </inputs> + + <outputs> + <data name="L2_tab" format="tabular" + from_work_dir="summarize_taxa_through_plots/*_L2.txt" + label="${tool.name} on ${on_string}: L2 tab"> + <filter>"2" in level and suppress_classic_table_output is False</filter> + </data> + + <data name="L3_tab" format="tabular" + from_work_dir="summarize_taxa_through_plots/*_L3.txt" + label="${tool.name} on ${on_string}: L3 tab"> + <filter>"3" in level and suppress_classic_table_output is False</filter> + </data> + + <data name="L4_tab" format="tabular" + from_work_dir="summarize_taxa_through_plots/*_L4.txt" + label="${tool.name} on ${on_string}: L4 tab"> + <filter>"4" in level and suppress_classic_table_output is False</filter> + </data> + + <data name="L5_tab" format="tabular" + from_work_dir="summarize_taxa_through_plots/*_L5.txt" + label="${tool.name} on ${on_string}: L5 tab"> + <filter>"5" in level and suppress_classic_table_output is False</filter> + </data> + + <data name="L6_tab" format="tabular" + from_work_dir="summarize_taxa_through_plots/*_L6.txt" + label="${tool.name} on ${on_string}: L6 tab"> + <filter>"6" in level and suppress_classic_table_output is False</filter> + </data> + + <data name="bar_charts_html_report" format="html" + label="${tool.name} on ${on_string}: Bar charts"> + <filter>'bar' is chart_type</filter> + </data> + + <data name="area_charts_html_report" format="html" + label="${tool.name} on ${on_string}: Area charts"> + <filter>'area' is chart_type</filter> + </data> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ +**What it does** + +This tool summarizes OTU by Category (optional), summarizes taxonomy and plots +taxonomy summary. + +More information about this tool is available on +`QIIME documentation <http://qiime.org/scripts/summarize_taxa_through_plots.html>`_. +]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> + +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/barcodes.fastq Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,1000 @@ +@HWI-EAS440_0386:1:23:17547:1423#0/1 +ATGCAGCTCAGT ++ +hhhhhhhhhhhg +@HWI-EAS440_0386:1:23:14818:1533#0/1 +CCCCTCAGCGGC ++ +ccc_c^_a[[JN +@HWI-EAS440_0386:1:23:14401:1629#0/1 +GACGAGTCAGTC ++ +ffdfcfffffcf +@HWI-EAS440_0386:1:23:15259:1649#0/1 +AGCAGTCGCGAT ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:23:13748:2482#0/1 +AGCACACCTACA ++ +ffffaffddffc +@HWI-EAS440_0386:1:23:6532:3028#0/1 +GAGAGAATGATC ++ +ghhhhhhhhhhh +@HWI-EAS440_0386:1:23:8677:3027#0/1 +CACAGTGGACGT ++ +eggggggggggg +@HWI-EAS440_0386:1:23:5678:3052#0/1 +ATAGCTCCATAC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:23:11889:3171#0/1 +ACGTTAGCACAC ++ +hhhhhhfhhhhh +@HWI-EAS440_0386:1:23:2112:3374#0/1 +GAGAGAATGATC ++ +eddaabddddcf +@HWI-EAS440_0386:1:23:5900:3393#0/1 +AGGTGTGATCGC ++ +fffefhhhhggh +@HWI-EAS440_0386:1:23:15468:3455#0/1 +GAGAATACGTGA ++ +ehghghfhhggg +@HWI-EAS440_0386:1:23:4405:3490#0/1 +ACGCGCAGATAC ++ +fffffaffffaf +@HWI-EAS440_0386:1:23:4674:3593#0/1 +ACATTCAGCGCA ++ +fggggggggggg +@HWI-EAS440_0386:1:23:15912:3842#0/1 +ATCAGGCGTGTG ++ +ccggggcgggff +@HWI-EAS440_0386:1:23:15732:4249#0/1 +CTCATGTACAGT ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:23:17444:4527#0/1 +AACGCACGCTAG ++ +hhhhghhhhhhh +@HWI-EAS440_0386:1:23:10725:4873#0/1 +AACGCACGCTAG ++ +gggggggggggg +@HWI-EAS440_0386:1:23:11691:4945#0/1 +ATCTTAGACTGC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:23:4723:5217#0/1 +CTCGCACATATA ++ +\acaYW^^aYYV +@HWI-EAS440_0386:1:23:13608:5705#0/1 +GATCGTCCAGAT ++ +chhhdhhhgheh +@HWI-EAS440_0386:1:23:5195:5979#0/1 +CAGCAGCACATA ++ +ggggggggggg_ +@HWI-EAS440_0386:1:23:1372:6057#0/1 +AGCTCTCAGAGG ++ +\aab`_ccc^ff +@HWI-EAS440_0386:1:23:2305:6385#0/1 +CGTATCTGCGAA ++ +hhhhhhhhhghh +@HWI-EAS440_0386:1:23:17333:6563#0/1 +CCAGTGTATGCA ++ +ggffgggggegg +@HWI-EAS440_0386:1:23:1091:6583#0/1 +CGATGCACCAGA ++ +c^ca_\\_]``a +@HWI-EAS440_0386:1:23:16169:6630#0/1 +CAACTATCAGCT ++ +hhhhhfhhhhhh +@HWI-EAS440_0386:1:23:14554:7030#0/1 +AGTTAGTGCGTC ++ +hhhhhghhhhhh +@HWI-EAS440_0386:1:23:14518:7351#0/1 +CTCGCACATATA ++ +gfgggggggggg +@HWI-EAS440_0386:1:23:17440:7360#0/1 +GCTGCTGCAATA ++ +ggggfggggggf +@HWI-EAS440_0386:1:23:11290:7687#0/1 +ATCGTACAACTC ++ +hhhfhhhhhhhh +@HWI-EAS440_0386:1:23:1465:8235#0/1 +CACATCTAACAC ++ +cc^ccccccccc +@HWI-EAS440_0386:1:23:3176:8324#0/1 +ACTGATCCTAGT ++ +add^dcda^ddd +@HWI-EAS440_0386:1:23:12535:8807#0/1 +GAAGCTACTGTC ++ +hhfhhhhhhhhh +@HWI-EAS440_0386:1:23:14562:8883#0/1 +CGAGTTGTAGCG ++ +fhhhhhhhhhhh +@HWI-EAS440_0386:1:23:5451:9226#0/1 +AGATACACGCGC ++ +hhhhghhhhhhh +@HWI-EAS440_0386:1:23:19204:9448#0/1 +GATCGCAGGTGT ++ +cccccc_ccTWU +@HWI-EAS440_0386:1:23:13161:10184#0/1 +CTACATCTAAGC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:23:19192:10519#0/1 +CATCGTATCAAC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:23:17370:10963#0/1 +GCTCAGTGCAGA ++ +gffggggfgggg +@HWI-EAS440_0386:1:23:8386:11532#0/1 +CAGCTAGAACGC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:23:10215:11663#0/1 +AGATCGGCTCGA ++ +ffffffffffcW +@HWI-EAS440_0386:1:23:15454:11726#0/1 +ACGTACTCAGTG ++ +ggggggggggff +@HWI-EAS440_0386:1:23:6589:11781#0/1 +AGTACGCTCGAG ++ +gggggggggggg +@HWI-EAS440_0386:1:23:3256:12101#0/1 +ATGCCTGAGCAG ++ +gggggggggggg +@HWI-EAS440_0386:1:23:5860:12157#0/1 +ACATGTCACGTG ++ +hhhghhhhhhhh +@HWI-EAS440_0386:1:23:8148:12187#0/1 +AACTGTGCGTAC ++ +hhghhhhhhhhh +@HWI-EAS440_0386:1:23:14399:12333#0/1 +ACATGATCGTTC ++ +ZTPQFILLJJKK +@HWI-EAS440_0386:1:23:6966:12378#0/1 +CATACCAGTAGC ++ +\aa_aaa^aa\^ +@HWI-EAS440_0386:1:23:5056:12637#0/1 +CGCATGAGGATC ++ +gggggggggggg +@HWI-EAS440_0386:1:23:2628:12653#0/1 +ACGCTCATGGAT ++ +cccccccccccc +@HWI-EAS440_0386:1:23:3303:12826#0/1 +AGGTGTGATCGC ++ +hffhhhhhhhhh +@HWI-EAS440_0386:1:23:11685:12866#0/1 +CTCCACATGAGA ++ +hhhhghhhhchh +@HWI-EAS440_0386:1:23:3932:12883#0/1 +CTACTGATATCG ++ +aZ]]]\Sa^a]_ +@HWI-EAS440_0386:1:23:3535:13218#0/1 +ACGACGTCTTAG ++ +hhhhghhhhhhh +@HWI-EAS440_0386:1:23:6909:13409#0/1 +AGTTCAGACGCT ++ +gggggfgggggg +@HWI-EAS440_0386:1:23:6191:13528#0/1 +GCTCAGTGCAGA ++ +ggggggeggggg +@HWI-EAS440_0386:1:23:17158:13561#0/1 +AATCGTGACTCG ++ +fffffafcccdd +@HWI-EAS440_0386:1:23:19363:13593#0/1 +CGGCGATGTACA ++ +hhhhhhhghahh +@HWI-EAS440_0386:1:23:10747:13717#0/1 +CCTCTCGTGATC ++ +ffcffdad\d\\ +@HWI-EAS440_0386:1:23:11158:13762#0/1 +AGCAGCACTTGT ++ +fc_ff_cdffff +@HWI-EAS440_0386:1:23:8303:13856#0/1 +CATAGCGAGTTC ++ +gggggfgaggff +@HWI-EAS440_0386:1:23:19671:14034#0/1 +CTGACACGACAG ++ +ggggggggdgcf +@HWI-EAS440_0386:1:23:3839:14047#0/1 +ATACACGTGGCG ++ +_\ffad[Waadd +@HWI-EAS440_0386:1:23:10766:14550#0/1 +CGCACATGTTAT ++ +hhhghghhhghh +@HWI-EAS440_0386:1:23:8747:14696#0/1 +GATCAGAAGATG ++ +fhhhghhhhhhg +@HWI-EAS440_0386:1:23:10687:14858#0/1 +CGACTTATGTGT ++ +ffffffffcfff +@HWI-EAS440_0386:1:23:11909:14865#0/1 +ATGGCAGCTCTA ++ +fffffffddfcW +@HWI-EAS440_0386:1:23:18778:15286#0/1 +ATTATCGTGCAC ++ +ffffdb`]_`]] +@HWI-EAS440_0386:1:23:1172:15688#0/1 +CGCGTAACTGTA ++ +ZFPFYSM\MK_a +@HWI-EAS440_0386:1:23:10432:15754#0/1 +AGATCTCTGCAT ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:23:15265:16137#0/1 +CGATAGATCTTC ++ +hhhhhhhhghhh +@HWI-EAS440_0386:1:23:3853:16425#0/1 +GCTCAGTGCAGA ++ +gggggggggggg +@HWI-EAS440_0386:1:23:4837:16715#0/1 +CAGCGGTGACAT ++ +hhfhhhhhhhhh +@HWI-EAS440_0386:1:23:15530:16858#0/1 +GAGCTGGCTGAT ++ +ffffWfffffcf +@HWI-EAS440_0386:1:23:10039:17071#0/1 +GCATCGTCAACA ++ +hhhhhhghhhgh +@HWI-EAS440_0386:1:23:12821:17733#0/1 +ACCAGCGACTAG ++ +gggggggggggg +@HWI-EAS440_0386:1:23:6118:18575#0/1 +ATACGTCTTCGA ++ +cffdfdfffff_ +@HWI-EAS440_0386:1:23:5450:18847#0/1 +CTGAGCAGAGTC ++ +hhhhhghhhfhh +@HWI-EAS440_0386:1:23:2535:18871#0/1 +CAGGTGCTACTA ++ +hfgghhghhhhh +@HWI-EAS440_0386:1:23:4582:18950#0/1 +GCTAAGAGAGTA ++ +hhhhhhdhhhgh +@HWI-EAS440_0386:1:23:15559:19608#0/1 +ATACTATTGCGC ++ +hhhhhhhhhghh +@HWI-EAS440_0386:1:23:9787:19631#0/1 +CGTGACAATGTC ++ +ggggaggggggg +@HWI-EAS440_0386:1:24:17851:2016#0/1 +CTACGCGTCTCT ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:24:12302:2593#0/1 +CTATCAGTGTAC ++ +WPQFQQJSRRTV +@HWI-EAS440_0386:1:24:8488:2754#0/1 +CACATTGTGAGC ++ +\\W\\VZYYUaZ +@HWI-EAS440_0386:1:24:6114:3818#0/1 +ATAATCTCGTCG ++ +ehhhhhhhhhhg +@HWI-EAS440_0386:1:24:13084:4634#0/1 +CGCATGAGGATC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:24:9705:4722#0/1 +AGTAGTATCCTC ++ +hhghhhhhhhgh +@HWI-EAS440_0386:1:24:6985:4749#0/1 +CTTAGCACATCA ++ +gggggfgggggg +@HWI-EAS440_0386:1:24:2227:6089#0/1 +ATGCCTGAGCAG ++ +gggggggfdggg +@HWI-EAS440_0386:1:24:7654:6279#0/1 +ACCGCAGAGTCA ++ +gggggggggegg +@HWI-EAS440_0386:1:24:11532:6376#0/1 +GCTGCTGCAATA ++ +dggggggfggga +@HWI-EAS440_0386:1:24:6005:6667#0/1 +CTATCAGTGTAC ++ +fdhchhhgffhh +@HWI-EAS440_0386:1:24:9083:6728#0/1 +AGTTAGTGCGTC ++ +fggggggggggg +@HWI-EAS440_0386:1:24:9947:7672#0/1 +ATACGTCTTCGA ++ +fffffggggggg +@HWI-EAS440_0386:1:24:5510:8037#0/1 +GCATGTGCATGT ++ +fhghghhhhhfh +@HWI-EAS440_0386:1:24:5505:8369#0/1 +CTCAGTATGCAG ++ +hhhhhhfhhhhh +@HWI-EAS440_0386:1:24:4692:8430#0/1 +CTCGAGAGTACG ++ +hhhhghfhgdhh +@HWI-EAS440_0386:1:24:7038:10072#0/1 +CATAGACGTTCG ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:24:18470:10081#0/1 +GCTAAGAGAGTA ++ +hhhhhhhhhhgh +@HWI-EAS440_0386:1:24:15353:10428#0/1 +GGACGCTCGACG ++ +gggggggggggg +@HWI-EAS440_0386:1:24:5578:10837#0/1 +AGACTGCGTACT ++ +hhhhhhghfhhh +@HWI-EAS440_0386:1:24:8169:11098#0/1 +CCAGTGTATGCA ++ +a^^\Y^]a]`ff +@HWI-EAS440_0386:1:24:15875:11235#0/1 +GCACGACAACAC ++ +hhhhdhhhghhh +@HWI-EAS440_0386:1:24:6113:13259#0/1 +ACTGTGACTTCA ++ +chhhehdhhhhc +@HWI-EAS440_0386:1:24:14722:13923#0/1 +GATCGTCCAGAT ++ +ggggggggfggg +@HWI-EAS440_0386:1:24:9379:14151#0/1 +ACGAGTGCTATC ++ +hhhghhhhhhgh +@HWI-EAS440_0386:1:24:3831:14326#0/1 +CTGTGACATTGT ++ +^]dcccffafff +@HWI-EAS440_0386:1:24:12505:14386#0/1 +GCTATCACGAGT ++ +ggggggggggfa +@HWI-EAS440_0386:1:24:2610:14604#0/1 +AGCGACTGTGCA ++ +gggggggfgggg +@HWI-EAS440_0386:1:24:3820:14809#0/1 +ATTATCGTGCAC ++ +hhhhhghhdhhh +@HWI-EAS440_0386:1:24:15451:14907#0/1 +GATCAGAAGATG ++ +fffddffff_fa +@HWI-EAS440_0386:1:24:18011:14951#0/1 +AGCACACCTACA ++ +gggggggggggg +@HWI-EAS440_0386:1:24:11603:15033#0/1 +CTACTGATATCG ++ +ggggggggggfc +@HWI-EAS440_0386:1:24:1920:15296#0/1 +ACGACGTCTTAG ++ +ggggdggggggg +@HWI-EAS440_0386:1:24:5025:15918#0/1 +CTGACACGACAG ++ +^c_ffggggggg +@HWI-EAS440_0386:1:24:3203:16220#0/1 +GCCAGAGTCGTA ++ +hhhhhghhhhhh +@HWI-EAS440_0386:1:24:4996:16249#0/1 +GACTGCATCTTA ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:24:14480:16254#0/1 +GACTTCAGTGTG ++ +hhhhhhghhhhh +@HWI-EAS440_0386:1:24:19188:16384#0/1 +ACATCACTTAGC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:24:17774:16420#0/1 +ACGGTGAGTGTC ++ +hhhhhhhhhghh +@HWI-EAS440_0386:1:24:15793:17037#0/1 +CGATCGAGTGTT ++ +gggggfffcfff +@HWI-EAS440_0386:1:24:15499:17067#0/1 +CCATACATAGCT ++ +gggggggggggg +@HWI-EAS440_0386:1:24:18506:17629#0/1 +ATACACGTGGCG ++ +ffffdfffc]d` +@HWI-EAS440_0386:1:24:3796:17704#0/1 +CGAGTTGTAGCG ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:24:6373:18472#0/1 +AGATCGGCTCGA ++ +ffffffffffa[ +@HWI-EAS440_0386:1:24:16235:18710#0/1 +CAGTACGATCTT ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:24:5656:19365#0/1 +CATCTGTAGCGA ++ +fggeggggcaff +@HWI-EAS440_0386:1:24:12256:19375#0/1 +CATATCGCAGTT ++ +hhfhhhghhhhh +@HWI-EAS440_0386:1:24:11025:19521#0/1 +ATACTCACTCAG ++ +hhhhghhhhhhh +@HWI-EAS440_0386:1:24:9307:19872#0/1 +ACACTGTTCATG ++ +hhhhhhehchhg +@HWI-EAS440_0386:1:25:1361:1248#0/1 +CCTCTCGTGATC ++ +aZ\\_VTV\TTa +@HWI-EAS440_0386:1:25:5646:1376#0/1 +ACATCACTTAGC ++ +gggggggggggg +@HWI-EAS440_0386:1:25:2107:1388#0/1 +AGCTGACTAGTC ++ +gggggggggggg +@HWI-EAS440_0386:1:25:1851:1463#0/1 +ATGGCAGCTCTA ++ +a^ffaff[f_aY +@HWI-EAS440_0386:1:25:3982:1463#0/1 +ATACAGAGCTCC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:4646:1592#0/1 +AGTGCGATGCGT ++ +aggggg_[ggfa +@HWI-EAS440_0386:1:25:4380:1628#0/1 +CCTCTCGTGATC ++ +hhghhhghhggh +@HWI-EAS440_0386:1:25:1510:1668#0/1 +CTGAGATACGCG ++ +hhhhghhghhhf +@HWI-EAS440_0386:1:25:3929:1715#0/1 +AGCTGACTAGTC ++ +hhhhhhhhhhgh +@HWI-EAS440_0386:1:25:4840:1834#0/1 +AGCTGACTAGTC ++ +hhhhhhhhhghh +@HWI-EAS440_0386:1:25:5558:1851#0/1 +AGCTGACTAGTC ++ +ff_ccc^^a[MR +@HWI-EAS440_0386:1:25:13850:1850#0/1 +ACACTGTTCATG ++ +hhhhhhhhhhfh +@HWI-EAS440_0386:1:25:2639:1894#0/1 +CCGACTGAGATG ++ +gggggggfgggg +@HWI-EAS440_0386:1:25:6139:2018#0/1 +AGCTGACTAGTC ++ +hhghhgggcahh +@HWI-EAS440_0386:1:25:3109:2059#0/1 +CTGAGATACGCG ++ +hhhhghhhhhhh +@HWI-EAS440_0386:1:25:6937:2060#0/1 +ATGGCAGCTCTA ++ +cYcYc\Z]LO\S +@HWI-EAS440_0386:1:25:7669:2080#0/1 +CCTCTCGTGATC ++ +hhhhhhhhhghh +@HWI-EAS440_0386:1:25:7091:2092#0/1 +CCTCTCGTGATC ++ +hhhhhhhhhfhh +@HWI-EAS440_0386:1:25:3922:2110#0/1 +ATGGCAGCTCTA ++ +ggcgggg]caf_ +@HWI-EAS440_0386:1:25:3806:2202#0/1 +AGTGCGATGCGT ++ +ffdffggggggg +@HWI-EAS440_0386:1:25:6540:2228#0/1 +ACTACGTGTGGT ++ +hhhhhhfhhhfh +@HWI-EAS440_0386:1:25:10019:2223#0/1 +CGACAGCTGACA ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:2668:2254#0/1 +CCTCTCGTGATC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:19646:2248#0/1 +ACAGACCACTCA ++ +hhghhhhhhhhh +@HWI-EAS440_0386:1:25:1802:2336#0/1 +AGTCACATCACT ++ +ghhhhhhhhhhh +@HWI-EAS440_0386:1:25:5054:2329#0/1 +ACTACGTGTGGT ++ +ghgghhchhhhh +@HWI-EAS440_0386:1:25:8482:2380#0/1 +CTGAGATACGCG ++ +_ffffafffdfg +@HWI-EAS440_0386:1:25:15658:2382#0/1 +GAGACAGCTTGC ++ +_ccaacacccac +@HWI-EAS440_0386:1:25:8261:2388#0/1 +CTGAGATACGCG ++ +hhhhhhhhhhhf +@HWI-EAS440_0386:1:25:9735:2401#0/1 +AGTGCGATGCGT ++ +hhhhhhhh_hhh +@HWI-EAS440_0386:1:25:8948:2437#0/1 +AGCTGACTAGTC ++ +gggggeggggcg +@HWI-EAS440_0386:1:25:12681:2438#0/1 +ATGGCAGCTCTA ++ +ggggggfcedfd +@HWI-EAS440_0386:1:25:12916:2459#0/1 +CCTCTCGTGATC ++ +hhhhhhhhhhfh +@HWI-EAS440_0386:1:25:7336:2484#0/1 +AGCTGACTAGTC ++ +bba]^addaacf +@HWI-EAS440_0386:1:25:1489:2530#0/1 +AAGAGATGTCGA ++ +eff]caffffd_ +@HWI-EAS440_0386:1:25:9578:2531#0/1 +ACTACGTGTGGT ++ +hhghhhghhgde +@HWI-EAS440_0386:1:25:9159:2552#0/1 +CCGACTGAGATG ++ +fffcfcadfaaf +@HWI-EAS440_0386:1:25:11396:2559#0/1 +AGCTGACTAGTC ++ +hhhhhhhhhhfh +@HWI-EAS440_0386:1:25:1760:2573#0/1 +CCTCTCGTGATC ++ +gggggggggadf +@HWI-EAS440_0386:1:25:14068:2621#0/1 +CCTCTCGTGATC ++ +hhhhhhhhfhhh +@HWI-EAS440_0386:1:25:8159:2639#0/1 +ACTACGTGTGGT ++ +hhgghghhfafe +@HWI-EAS440_0386:1:25:13134:2667#0/1 +ACACACTATGGC ++ +hhhhhhhhghhh +@HWI-EAS440_0386:1:25:11175:2690#0/1 +ATGGCAGCTCTA ++ +fffdffff[]]\ +@HWI-EAS440_0386:1:25:8576:2709#0/1 +CCGACTGAGATG ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:6943:2731#0/1 +GACGCTAGTTCA ++ +ggggggagggfc +@HWI-EAS440_0386:1:25:1850:2741#0/1 +AGCTGACTAGTC ++ +f^ffffcfdffd +@HWI-EAS440_0386:1:25:10428:2744#0/1 +AGTGCGATGCGT ++ +hhhhhhhhhhgh +@HWI-EAS440_0386:1:25:12398:2774#0/1 +ACACACTATGGC ++ +hhhhhhhghhgh +@HWI-EAS440_0386:1:25:14110:2805#0/1 +ACTACGTGTGGT ++ +ghhhhhhhhhhh +@HWI-EAS440_0386:1:25:14127:2819#0/1 +ATGGCAGCTCTA ++ +gggggggfaaf_ +@HWI-EAS440_0386:1:25:1968:2841#0/1 +ACACACTATGGC ++ +ffff_fffffgg +@HWI-EAS440_0386:1:25:10866:2839#0/1 +AGCTGACTAGTC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:11767:2838#0/1 +AGTGCGATGCGT ++ +hgghhhghhghg +@HWI-EAS440_0386:1:25:4402:2852#0/1 +AGTGCGATGCGT ++ +gghhhhghghhh +@HWI-EAS440_0386:1:25:1421:2878#0/1 +AGCTGACTAGTC ++ +fdfffgggcgff +@HWI-EAS440_0386:1:25:11319:2883#0/1 +CCGACTGAGATG ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:3648:2910#0/1 +AGTGCGATGCGT ++ +ghhhhhhhhhhg +@HWI-EAS440_0386:1:25:6586:2930#0/1 +CCTCTCGTGATC ++ +hhhfhhhgghhh +@HWI-EAS440_0386:1:25:8471:2931#0/1 +AGCTGACTAGTC ++ +hhhghhghhhhh +@HWI-EAS440_0386:1:25:14208:2940#0/1 +ACTACGTGTGGT ++ +hhhhhhhhhghg +@HWI-EAS440_0386:1:25:2027:2951#0/1 +AGCTGACTAGTC ++ +gcggggeggfdf +@HWI-EAS440_0386:1:25:13175:2941#0/1 +GCTGCTGCAATA ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:13489:2951#0/1 +AGCTGACTAGTC ++ +hhhhfhhhhhhh +@HWI-EAS440_0386:1:25:6132:2959#0/1 +ACTACGTGTGGT ++ +a`aY]ada^e\L +@HWI-EAS440_0386:1:25:2692:2971#0/1 +ACACACTATGGC ++ +hhghhhhhhfhh +@HWI-EAS440_0386:1:25:1831:2983#0/1 +ACACACTATGGC ++ +ggggcggacg^[ +@HWI-EAS440_0386:1:25:6335:2989#0/1 +CCTCTCGTGATC ++ +afdeff\ffaca +@HWI-EAS440_0386:1:25:10162:3028#0/1 +ATGGCAGCTCTA ++ +fffffdfRc]^[ +@HWI-EAS440_0386:1:25:4348:3042#0/1 +ATGGCAGCTCTA ++ +ffa_fffa_d\J +@HWI-EAS440_0386:1:25:5972:3048#0/1 +CTGAGATACGCG ++ +hhhhhfhhhghh +@HWI-EAS440_0386:1:25:9861:3050#0/1 +ATGGCAGCTCTA ++ +gggggggcfcea +@HWI-EAS440_0386:1:25:10090:3050#0/1 +CTGAGATACGCG ++ +ghhhghhhghhh +@HWI-EAS440_0386:1:25:3139:3077#0/1 +AGCTGACTAGTC ++ +gggggggggggg +@HWI-EAS440_0386:1:25:9110:3081#0/1 +AGTGCGATGCGT ++ +hhhhhhghhhhh +@HWI-EAS440_0386:1:25:17734:3100#0/1 +ACTACGTGTGGT ++ +ggggggggggfc +@HWI-EAS440_0386:1:25:8423:3141#0/1 +ACACACTATGGC ++ +hhhhhhhhhghh +@HWI-EAS440_0386:1:25:14009:3149#0/1 +AGCTGACTAGTC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:3100:3158#0/1 +CCGACTGAGATG ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:4738:3152#0/1 +AGTGCGATGCGT ++ +ggggggggggfg +@HWI-EAS440_0386:1:25:4197:3165#0/1 +CGAGAGTTACGC ++ +hhhhehhhhhhh +@HWI-EAS440_0386:1:25:9072:3171#0/1 +CCGACTGAGATG ++ +gggg_ffdffgg +@HWI-EAS440_0386:1:25:15751:3166#0/1 +AGTGCGATGCGT ++ +fcccffdfffgg +@HWI-EAS440_0386:1:25:3473:3225#0/1 +CTGAGATACGCG ++ +gfgfggfggggf +@HWI-EAS440_0386:1:25:4755:3229#0/1 +CTGAGATACGCG ++ +hhhhghhhhghh +@HWI-EAS440_0386:1:25:3032:3237#0/1 +CCTCTCGTGATC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:1630:3249#0/1 +CTGAGATACGCG ++ +hhhhhghhhhhh +@HWI-EAS440_0386:1:25:4476:3251#0/1 +AGCTGACTAGTC ++ +hghhhhhhhhhh +@HWI-EAS440_0386:1:25:3312:3259#0/1 +ACTACGTGTGGT ++ +hhhhhhhhghhe +@HWI-EAS440_0386:1:25:3550:3291#0/1 +ACACACTATGGC ++ +hhhhhhhhghhg +@HWI-EAS440_0386:1:25:5478:3298#0/1 +CCGACTGAGATG ++ +hhhhhhhhhfhh +@HWI-EAS440_0386:1:25:15346:3296#0/1 +CCTCTCGTGATC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:3468:3322#0/1 +ACTACGTGTGGT ++ +fhdfhhhhgdfe +@HWI-EAS440_0386:1:25:4199:3321#0/1 +CCTCTCGTGATC ++ +hhhhhhghhhhh +@HWI-EAS440_0386:1:25:8568:3330#0/1 +ATGGCAGCTCTA ++ +gggggggf_fdd +@HWI-EAS440_0386:1:25:12050:3335#0/1 +ACACACTATGGC ++ +gggggggggggg +@HWI-EAS440_0386:1:25:19075:3342#0/1 +CAGTACGATCTT ++ +hfhfehgghhhh +@HWI-EAS440_0386:1:25:4994:3354#0/1 +ATGGCAGCTCTA ++ +gggggggffef[ +@HWI-EAS440_0386:1:25:12091:3381#0/1 +ATGGCAGCTCTA ++ +ffffffffcaa_ +@HWI-EAS440_0386:1:25:8594:3395#0/1 +AGTGCGATGCGT ++ +hhhhhhhhhhhf +@HWI-EAS440_0386:1:25:2052:3400#0/1 +CCTCTCGTGATC ++ +ggggggggfgfg +@HWI-EAS440_0386:1:25:11776:3405#0/1 +ACTACGTGTGGT ++ +hhhhfhhfhe[f +@HWI-EAS440_0386:1:25:4155:3413#0/1 +AGTGCGATGCGT ++ +fffffggggggg +@HWI-EAS440_0386:1:25:11052:3431#0/1 +ACTACGTGTGGT ++ +hhhhhhghhhhe +@HWI-EAS440_0386:1:25:4122:3435#0/1 +ATGGCAGCTCTA ++ +c_cYc`WKZP\Y +@HWI-EAS440_0386:1:25:9796:3444#0/1 +AGTGCGATGCGT ++ +hhhchhhhhhhh +@HWI-EAS440_0386:1:25:2235:3462#0/1 +AGCTGACTAGTC ++ +fcaffffcfcgg +@HWI-EAS440_0386:1:25:14529:3458#0/1 +CCGACTGAGATG ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:6708:3509#0/1 +ACACACTATGGC ++ +ffffdfffffcf +@HWI-EAS440_0386:1:25:13919:3507#0/1 +CCTCTCGTGATC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:10705:3535#0/1 +ACACACTATGGC ++ +hhhfhhghgfgh +@HWI-EAS440_0386:1:25:7788:3570#0/1 +CTGAGATACGCG ++ +hhaghghhhdhh +@HWI-EAS440_0386:1:25:2273:3577#0/1 +ACTACGTGTGGT ++ +ffffffdfffe_ +@HWI-EAS440_0386:1:25:14850:3657#0/1 +CCGACTGAGATG ++ +aaYc^aaYZZYT +@HWI-EAS440_0386:1:25:11581:3662#0/1 +ACTACGTGTGGT ++ +hhhhhhhhhhhg +@HWI-EAS440_0386:1:25:16965:3660#0/1 +ACTACGTGTGGT ++ +hhhhhhhhhhhg +@HWI-EAS440_0386:1:25:8197:3691#0/1 +CTGAGATACGCG ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:11617:3701#0/1 +AGCTGACTAGTC ++ +`\__\W\\\Y\^ +@HWI-EAS440_0386:1:25:15484:3705#0/1 +CCGACTGAGATG ++ +hhhhhhhhhhhh
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/forward_reads.fastq Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,1000 @@ +@HWI-EAS440_0386:1:23:17547:1423#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGGATTGGTGTG ++ +hhhdHddddddddfehhfhhhghggfhhhfhhgggfhhgfgdfcfhehfdgfhggfggfggffgddfgdffdgdaagaaddcbdccc]a^ad__a]_____ba_`a`__^__\]^OWZR\Z\\WYTZ_U^BBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:14818:1533#0/1 +CCCCNCAGCGGCAAAAATTAAAATTTTTACCGCTTCGGCGTTATAGCCTCACACTCAATCTTTTATCACGAAGTCATGATTGAATCGCGAGTGGTCGGCAGATTGCGATAAACGGGCACATTAAATTTAAACTGATGATTCCACTGCAACAA ++ +US[QCQSZPHNYIa[^aaccaac[]accBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:14401:1629#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGGGTTGGGGG ++ +fffbF`bbWZZ]Zggggfgcgggggddggdgggggdbgdddgbgeggg`ffdggeabba`aaad]]]d\]`]`[]]aWaYa\aa`a`_````_^]^^^]]]X]_``____``BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:15259:1649#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGAGTTTGGGGG ++ +hhhdHcdd^bbbahhhhhhfhhhhhghhhhhhhhhghhhgehchfhhhgghghfhhhgehgaffaecfdggdh\bafadddddgddfdbc^]a_\^_a``\X`^_`]`aaabcaYbY_^^X]^ZY^Z^a`_^aBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:13748:2482#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGCGGGATTCGTGGTG ++ +fffbF^bbS[T[Uggggggg__gffddfggeggggaagfeefbfaaff_cfacfeceddgggegdgdgaggddcddd`a_aad`ddadd`daaWY]]Yddda_]_]]a_Y___X\_]YaY]\]WYNV]\]_BBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:6532:3028#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGAGTCTTGGGG ++ +hhhfHdffacdcchhhhghfhhhhfehhhchhhehghhgghhgehghhffghhhgghghddgggfhfgdhhdea`Wf^dddddbgagcdedgcfddbcddaddcefb_addddd_daaaaaaa`ca`aX^Z\X_^^^^BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:8677:3027#0/1 +TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGCCGGCACATGATACTGTGAGACTAGCGTAACGGAGGGGGAACCGGAATTCTTGGTG ++ +ggg`Fbbbdeeeegggggggggggfgggggggggggggggggfgggggggggfgggggggf]gfccfcfbfbdged]ddda_dedaa]aZc__BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:5678:3052#0/1 +TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGAGCAAGTCTGATGTGAAAGGCTGGGGCTTAACCCCAGGACTGCATTGGAAACTGTTTTTCTTGAGTGCCGGAGAGGTAAGCGGAATTCCTGGGG ++ +hhheHeeeaaabbhghhhhhhhhhghhhhghhhhhfhghhghhghhhhdhehhfghhaehhgghddhhhhhadffffdb]Z__Z^_`^daadda`c`Z\T[\\]\RJ]YS[\X_a]]P_XM\T\^\^^BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:11889:3171#0/1 +TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTATGACTAGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTG ++ +hhheHddeeeeddhghhhhhhhhhhchhhhhhhhhfhfdddffhgghhghgehhegghhhhffhffhhg_edaecdcc[dabaadddaadaaeY\WQX^]]^^``RZZ___a__aa_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:2112:3374#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTCGTGTGG ++ +fffdGcddZZ___ggggggggggggggggfggggggfgdggggeggggeggggggeggdggeggggggdggdgcaaf\^_b_b_gdedd_eedR^Z^^`dcRb_P__R^\[]^c`cbadaaab^da^aBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:5900:3393#0/1 +TACGNATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTAGAGTGTCGGAGGGGTAAGTGGAATTCCTAGTG ++ +hhheHeeea\aaaghhhhhhhhhhhhhhhhghhhhfhgggghhhhhhfcghhhhgfhdgahddfbddfcfca]daed]cfcdcb]bbb^]_^bUZRYW___^_abaV_^SYYW````]^_[O_^```Z\RS^BBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:15468:3455#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGGGGTGGGGGGGATTCGGGGGG ++ +hhhdHaddddedehhhhghhhhhhhchhhghhhghghhgfghhddfdhggfhfhehghhfhgghdhehghhfgaaaf_cacaadaadbb`bcaacabcdbecc`ddaaa_aabacaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:4405:3490#0/1 +TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCGGAGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACGGCTTTTGAAACTGTCCTGCTTGATTTCAGGAGAGGTAAGCGGAATTCCTAGTG ++ +fffdGdcdbabbbggggggggggggggggggggggggggggggfggggggggggfggWffcfdgdggggggdfdeWadggededacgegdddgcd__da_c`XebdadedaedaJaaaW``_RZW^[W]Q^BBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:4674:3593#0/1 +TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGGCGGCACATGATACTGCGAGACTAGAGTAATGGAGGGGGAACCGGAAATCTCGGTG ++ +gggUDW_Y[a^^aggggggggagfffffcffeeccffabbbbcfefffdeggdggeggggddda_cf_bY__`bbbaa_cccaaL^aBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:15912:3842#0/1 +AACGNAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAAACTATGGGCTCAACCCATAAATTGCTTTCAAAACTGCTGGCCTTGAGTAGTGCAGAGGTAGGTGGAATTCCCGGTG ++ +fff\Faaa`babbggggggggggggggggggggggggggggggggggfggggggg`ggcdfdegegggfafdfgggdgggggfa_eggaddbdaaf]`fecddgdddb[``a\aaa\`bddbdacb]caacBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:15732:4249#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGTGTGGGGGG ++ +hhhdHdee`bbaahhhhhhghhhfddffhehhhhhehhhdhhhaeddhghfheh_gghghedehggdhghggfaacfaedf_fabddaaa_fab_```decdhcegdW[_b`bdaeaa^b]W`[`]``BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:17444:4527#0/1 +TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGATGTGAAAGGCTGGGGCTCAACCCCGGGACTGCATTGGAAACTGCCCGTCTTGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTG ++ +hhheHdedfffffhghhhhhhhhhfffhhhghhhhfhfggghhghhghgggfghhhfggfdffgachh]ca_aaabdd_cdead]_aa_]]_baaa_a_a_]_\YJWZX\U\Z^^^U_a``^a\aBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:10725:4873#0/1 +TACGNAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTAGGCGGTTGGTTAAGCGTGTTGTGAAATGTAGATGCTCAACATCTGACTTGCAGCGCGAACTGGCTGACTTGAGTACACACAACGTAGGCGGAATTCATGGTG ++ +gggdGbcdb^b`\fffffgggggggdggggggggggggeeefffcfdfgggggggggggcgfga[_cfdfeb_`_bbdddeefdbdccdgaggggdafcddbgdeaeaaaaabdgdda]]b_bccecacaaBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:11691:4945#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATACGTGGTG ++ +hhhbHbbbcdddchhhhhhhhhhhgghhhhhhhhhhhhhgchhghhhhhhhfhhghhhhhhgghghfhghhdhdcdhcgghghfbggdedghedcdah]dbaacdgddcaeee]dd_ac_``_TT[T[Z_XZV_^W^BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:4723:5217#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACCGTAGGGGTGGGGGGAATTTGTAGGG ++ +NRRKD\ZQVUVSTcccccccbccccccccc_cccccaccccccccccc_ccc_bcc[[cccccccc[caccccc^ccP]Z`Z_ccccccccccHQGYVa_acccccYc_ccccI[Va]\\^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:13608:5705#0/1 +TACGNAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTAGGCGGCCTGTTAAGCGTGTTGTGAAATGTAGATGCTCAACATCTGAACTGCAGCGCGAACTGGCTGGCTTGAGGACCAGCAACGTGGGCGGAATTCATGGTG ++ +hhhbH`ba_Z_a]^ffffhgd]hffdffhhhhghhbhhdedfffbfef_acfcdddadgaaeeaRcaa```^`___ca_aaabcbcabdccdcacaaa_Zb__\__[___Z]_aabBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:5195:5979#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGATGTGGGGGG ++ +gggbF^bbSa\_\gggggffaefffdafgggggggdggeedfafa[cfdgeggggggg]ggddgdgegdggegabad`dbbbcdddbdd`adT]TY]_]_Z]`aae`c]]bYd]Z`WWaa_]aBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:1372:6057#0/1 +TACGNAGGTGGCGAGCGNTGTCCGGAATTATTGGGCGTAAAGGGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGT ++ +fffcGcccZY]]Y`abaEccaccfffdfffWffffffcfaffafffffcfffcafbdfc_ccefccfc^W^^abbcacYYX^Vccaccdc`b]^ZP^X^M^Z^`W]W]a_aZa]^MX]]]M]W`_XW`BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:2305:6385#0/1 +TACGNAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTGATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTGTGTTTGAGGTAGGCGGAATGCGTGGTG ++ +hhhaH`bbddddchghhhhehhhhgahhhehhhfhhhhddcfffdhfhfcffdhhefhhhhhghghggehebh^[\d\fcfacggdddfffchb_dXc``_afaa_aaadbcddadaa^\RH\JSISO___Z_BBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:17333:6563#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTCGTGGGG ++ +gggdGaddabbbaggggggcgggggacgggggggggggggggggggggeeggfgggdggeggfcaacfecfdfgeQgedgggefedfegffgfgfbffgffcfadg]daadc][aa_aaca_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:1091:6583#0/1 +TACGNAGGTGGCAAGCGNTATCCGGATTTATTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGNTNNNNNNCCNNCCTGCATTTGAAACTGNAGTNCTTGAGTGTCGGANNNNNNANCGGNATTCCGTGTG ++ +\`^WF`UY^RW[\ab`aEacdcafffceffaccffdfffffdff]fRfd^eeeffccfReaaaebbKef`ffff]fafda[ddPCVDDCCCCYFDD]XZ]\VZfRcc`TYZ_DQT^DU`^`O^c[cfBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:16169:6630#0/1 +AACGNAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTG ++ +ghheHeeeaddcdhhhhhhfhhhhghchhhghhhhgghhhghhghhhffehfcgghghggdfghdhh_dddbdddghbfggddc_dfadad`ddaba_gddadeddabaadeaadbb``eb]caaaabaad]ba_W^_Z_ZQZR\UZ\Y[TY +@HWI-EAS440_0386:1:23:14554:7030#0/1 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+gggdGdddddccdggggggggggggfgggggggggggggggggadddffdfffgggffegggdggggg_ddb_ebddeebgcgfggff_dePdaaca_dddgdff[daadccaa]aaaaaKaa_aT\a\Z[^RY\\\\]_BBBBBBBBBBBB +@HWI-EAS440_0386:1:23:11290:7687#0/1 +TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGAGTAATGGAGGGGGGACCGGAATTCTCGGTG ++ +hhhdFeee^aaaaghhhhfdb_dfddcfghfhhhhfhhhfhhccffffhhhhcfhhhhhhggefdff_fefefdddbe[ddedc_Zaa^[^^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:1465:8235#0/1 +TACANAGGTCTCAAGCGNTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGGGAGACTTGAGTAATGGAGGGGGAGCCGGAGTTCTCGTGG ++ +cccLD]YZVZ]]]aaaaDcccccccccccccccccacccccccccccccccacccccccccccccccccccccccccbTTP]]cBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:3176:8324#0/1 +TACGNATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGATGTGAAAGGCTGGGGCTCAACCCCGGGACTGCATTGGAAACTGCCCGTCTTGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTG ++ +ddd]D`__Z^Y]UfffffffffffffffffWfffffafffffffdffffffccfffff^ZO^^dad]d^dd^dcfcf[IZ`Z^ffcfededa`^WZZWcfaaaXTWYH^^^^SWM^X]cbbacacb[c[H^BBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:12535:8807#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGGCGGGATTGTGGTGT ++ +hhhcH_dcaddacghhhehghhhghdhhhdhhghhdhhdeeeegfhggfgffgdghgghfffffcfggfgedfaaad_dcdcadaddab`ddaddaacaaaae_ca]aZ__ZaaaaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:14562:8883#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTCGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGGATTTGGGGTG ++ +hhheHdeebbbbahhhhhhfhhhghghhhhhhhhhhhghhhhghghghehhfchhhhhhhhhhfghhhdhhehdc]hehgggehfaffddahdececcdd]dgac_bbcabcddbgcd]ca_aZ^ZOZVV[Q[a_aBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:5451:9226#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGCGGGATTCGGGGTG ++ +hhheHeeedeeeehghhhhhhhhhhghhhhhhhghghhhhhhhehhehghhhhhhhhhghhhhhfgghghhgheedfeegheggfdhfcdahegddgefgcdfdfg[ca_dddcdcea[dbcbaadaedaaaa]^^aBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:19204:9448#0/1 +TACGNATGGAGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGTACGCAGGCGGTTTAATAAGTCGAATGTTAAAGATCGGGGCTCAACCCCGTAAAGCATTGGAAACTGATAAACTTGAGTAGTGGAGAGGAAAGTGGAATTCCTAGTGT ++ +b^^aD^aaccccccccccccccccccccccccccccccccccccaccccccccccccccccccccccacccccacccc`cbcbc`aaacaa_ac_a__a^accca[_[X__Z_NRMJT__Z_VX[X[aY_BBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:13161:10184#0/1 +TACGNAGGGAGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGATCTGCAAGTCAGTAGTGGAATCCCAGGGCTTAACCCTGGAACTGCTATTGAAACTGTGGGTCTTGAGTGAGGTAGAGGCAGGGGGGATTGCCGGGG ++ +hhhaHddccdccchhhhhhhhhhhhfehhhhhhhhehhhhhhhhghdhghhhhhhhhhhgchghdhfcddddf_acffeghheefghdgagbhhgddhegdggehhaa_]`b_dedab`[`__adaabY]^\_Z^Z^BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:19192:10519#0/1 +TACGNAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGGCTTTTAAGTCTGACGTGAAAATGCGGGGCTTAACCCCGTATGGCGTTGGCTACTGGAAGTCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGT ++ +dhh[Ga\\daacchhhhhhdhhhhhhhhhhhhhfhghhchdhhhhhhhhgfhhhhhghhhhcchhhhhhhhhhdhehhghchhhghdh_fcWcfaccf``d`]UPIWMYZSYW]\L[Q\[Z[Va_a_[^_BBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:17370:10963#0/1 +AACGNAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGACCGGCAAGTTGGAAGTGAAAACTATGGGCTCAACCCATAAATTGCTTTCAAAACTGCTGGCCTTGAGTAGTGCAGAGGTAGGTGGAATTCCCGGTG ++ +fggdGeeedccccggggggggggggggggggggggggggggggggggeggg[gggg]daafcffedfcaafffeddaefdddcddcdegggcffWdafddedcgg]g]caff[aac[cccf]c_aR_a[cc[ca]aacV^BBBBBBBBBBBB +@HWI-EAS440_0386:1:23:8386:11532#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAGGTAGGGGGAGTTCGTGGTG ++ +hhhbH^ba\aaaaefhhhhghhhhhefhhehhhhhghhhghggehgggeghdghhe`gfghgggdgghfggfgfdcgdffgfccfbfeddcfcfccdfdfcdfaee]a_]\\_]^Y^\b__]]\_aa\_\a_aBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:10215:11663#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGTGGGGGGGG ++ +fffbF`bb`bbbaffffffffffffefffffccfffffffffffefffefffdffefffefedfdeefedddf[aTc_ddedecdddddddcaddaadccY_cbddad]aaacZQ^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:15454:11726#0/1 +TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGGCGGCCCATGATACTGCGAGACTAGAGTAATGGAGGGGGGAGCGGAATTCTGGGGG ++ +fffbFbbb`abbagfgggggggggggggggggggggggggcgeggggfe[fffgggfeba`b`fcaccfacafccacaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:6589:11781#0/1 +TACGNAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGCTATGCAAGACAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGTGACTGCATGGCTAGAGTTTGTCAGAGGAGGGTGGAATTGCGCGTG ++ +gggaFaaa^\\aafffffggcggffd_fgggggffefffcfffggggfgggggggggggggfafcfdf[dfdcddddfbfdeddddfbcc_ff_b]`abb`[bgegd_cddac_ggdddcc_[]``]]X]`Z]^VZ\\W_\OIRTEWWV^X^ +@HWI-EAS440_0386:1:23:3256:12101#0/1 +TACGNATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTTGAAACTGCAGGGCTAGAGTGCAGGAGGGGCAAGTGGAATTCCTAGTG ++ +gggZE_X\\a\aaggggggggggggggggggggffefefddfffdfffggggggaggggggg]fdfefdegdgbacg]bddbdd`aaV_^aa^TXSOU__\_aaaa\_`aaa^U]\_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:5860:12157#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTGGGGGGG ++ +hhhdHadd\```ahfhhhhghghhgffhhghhhfhfhhggfhhdafdfeehbhhgfghfhgffhffdhfggfgddcgcfefbgaffdcdaaedb_aabdfcdcdefcddae[c`c_`\acaabcdccfaa^^^^FX^BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:8148:12187#0/1 +TACGNAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGT ++ +hhheHdee^bb``ghhhgfadefhgdahhghhhhhghfhgghhghhfhgggfdgffgggfhfegddgggadagdabeecdaeab`dacaaaaad__bd__b_aa_\_\SY\[YaaZaa_a\_\a][^aXXFX\Z\Z^\_BBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:14399:12333#0/1 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+gggeGdeebbbbagggggggggggggggfdffcffeffgggggggggggggggggfggggggfdcddfdfcdfbbafbdefcdgfefdcb_eabbabdceacfadda_bad_dbad_a]`]`Z\aa_`_d___U_]Y_BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:2628:12653#0/1 +TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGGCGGCACATGATACTGCGGGACTAGAGTAATGGAGGGGGGACCGGACTTTTCGGGG ++ +cccLD[[]]bbbacccccccccccccccccccccccccccccacccccccccccccccccccbc_cc_aBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:3303:12826#0/1 +TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAACCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGAACTAGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTG ++ +hhhbH`bbbbbb`hhhhhhhhhhhhhghhhhhhhhehdgdghghghhhgghghhfhghgghefgddheeggagdcdgefdcfcd[ce]aaaaadcaX`aaadacacWa^Z^]Zaa_aR^]^VZZZ\\^BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:11685:12866#0/1 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+hhheHbdd]````ghhhfhehghhfdhhhdhhgghdhhfdffgddgehdbhdheecdhffhfegff`gdgddga\_c_dcdcdddccbbaadaaaaacdda`c`ab]aY\_a__aa^_aaa__]_a]bZX]T^_Z_\ZK^BBBBBBBBBBBB +@HWI-EAS440_0386:1:23:6909:13409#0/1 +TACGNAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTAGGCGGTTGGTTAAGCGTGTTGTGAAATGTAGATGCTCAACATCTGACTTGCAGCGCGAACTGGCTGACTTGAGTACACACAACGTAGGCGGAATTCATGGTG ++ +gggeGddea[aa\fffffgggggggbggfeffeggggggggggfdf_fdeeffggfgafcfdd[caafggggecdggggfgggffggcdadgggcdcgcdaagddb]ba__]cba_bacdbcdcc`aabaaVJ____\Z_BBBBBBBBBBBB +@HWI-EAS440_0386:1:23:6191:13528#0/1 +AACGNAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTG ++ +fggdGadd`deeeggggggfgggggffggggggggfgggggggggggegggggggggeggggggfgggeagdggfbggeaggbdbddaaa_dbec_cddddddfdccddd^fccce````badaa_aaa_aV_`Z]]]Q\]PW\\\\V\\T_ +@HWI-EAS440_0386:1:23:17158:13561#0/1 +TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGGCGGCACATGATACTGCGAGACTAGGGTAATGGAGGGGGGAGCGGGCTTCTCGGTG ++ +eee_D]``\aaaagggggggggggggggcgggggegggggggaggggggggggfgggggggffdddfafacacaYaaaad[bb`ZZ]\a_bY_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:19363:13593#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTATAATTGCAGTTGATACTGGCGACCTGGAGTGCAACAGAGGCAGGCGGAATTCGTGGTG ++ +hhhbH`bbeeeeehfhhhhghhhhhchhhfhhhghghheehghfhfhhhhhc_hhgbhhgfgahhggghhgfacaafbddgdhbeagabaafecccadd_dadd\bad^^__aBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:10747:13717#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTACAGTAGAGGCGGGGGGGATTCGTGGGG ++ +[`a_Ea_^ccaccffffffffffffffffffccffffffffffafffffffcfffff_cfffcfefffffffffdeffffffccefecddefdddedceaeafeff_caccccJS^ZWZYOVV]``]bYaaYa^BBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:11158:13762#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTCGGTGGG ++ +fffcGdcc`abbbggfgggggdggggggggggggggggggf_fgggeggggg]gggdgcgfggcgggdfffdfbdbcdgegadgfgdbedageddaddgecggbeaaeabcdbc__dW_aT]_]_V``__BBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:8303:13856#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGAGTGTGGGT ++ +fffdGcdd\aaaaggggggagggffddfggggggggggggggcggggggggcggfeggeggggggffgcfecfdbdddcccacegfgdeffeccdddefgfdf`ggadbaddd[Z`]\`b`W]]]\``ba__BBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:19671:14034#0/1 +TACGNAGGATCCGAGCGNTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGGTTTGTGGTG ++ +fffdGddd\aaaaacddFededegfagggggggggggggggggcffffggggggffeggeegggggffgged_```b`acfccabdcaaaWfa]_aaaffbdfc_[a]]_b`]_d]]__]S]`aa]abBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:3839:14047#0/1 +TACGNAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGCTTCCTAAGTCCATCTTAAAAGTGCGGGGCTTAACCCCGTGATGGGATGTAAAATGGGAAGCTGGGGGTTCGGATGGGGAAGGGGGATTTCTTGGGG ++ +ddddGdddTVY]ZfffffffffffffffffffffdfcfffdfffffffffffcffcfQ^[dadfce]]a]adW````]cacce[cf_cccaaO^U^Z^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:10766:14550#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTTGTGGGG ++ 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+TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTTGGTGTG ++ +fffbFbbbaddccffffffafffffefffffccfffffffdfffdfffeeffde_ffffdffefdeefdfdefdddfdfdddddcdddbcafddddddddddd`e`bda_a_[ddd_cbdbOb_]\aa\aa]aaaaJ`BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:18778:15286#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGGG ++ +ddd^E_a\addccgggggggggggfcgggggggggggggfggggggggfeffcggggggggfffdfdfgggggdddgdgggdgdYdcdbaadaddddgffdcgaggad_a]aaccfcc]cc_a_\__a_\aTaWS^BBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:1172:15688#0/1 +TACGNAGGATCCGAGCGNTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTGCAGTTGAGGCAGGGGGGAGTTGGGGTG ++ +W_WQF^NN]QN]NcZROEQ`U^NXX\XX[f[Qbe_f__d[^V``dTdVffYfb`efddafY_daff^[feefcfWccffffffafafdfffbfa\dddfaccffffW_ccddcKaaa[Y\aK_T\`W_V`bZ^a^Vb^BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:10432:15754#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGGTTTGTGGGG ++ +hhhbH`bbaddcchhhhhhghhhhhghhhhhhhhhghhhgehhhdhdhddfdfhhddhhhhgghdeehghhggdccheeggdgadada``^d`aa]aaddbbe_ddad]^b`badaaa_dd_bad_]da`_\_^\[_^BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:15265:16137#0/1 +TACGNAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTGGTCGCGTCTGTCGTGAAATCCTCTGGCTTAACTGGGGGCTTGCGGTGGGTACGGGCCGGCTTGGGGGCGGGAGGGGGGGAGTGGAACCCTGGGG ++ +ghhaHcdc_aacchhhhhfcfffhfhahhhhhhhgfhhghhhgghggghhhghghhghhgccdfcaffdaffddhdhd]ddffa_cc_[[```hea]e^^_d_]a^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:3853:16425#0/1 +AACGNAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTG ++ +gggdGccddeeedgggggggggggggcggggggggdgeeedffegggggggggegegggdgfcc_ffcccaacaddgeeggddadagd_abcddaaaadc_gaaebaaaaac_dcbb``bb_aaa_^\YXZLUa\\__BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:4837:16715#0/1 +TACGNAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGGACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTG ++ +hhhdHaddcaccdhghhhhhfghhhhdhhhhhhhghhhffffafffffhhh]hgggghcdhhchfhheghhfdgffeggghgadddggde]fc_`Qcb_acaadce^ddaaadddaba_b__bedddd__aOa__`BBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:15530:16858#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTTGTGGTG ++ +fffcGccc`abbbffffffffffffafffffccfffffffffffffffeefefffcefdfdfffcccfbffcfcccfcfdddcdcedb]bbebacdd[bcaacda[addbdc_WZ`ZZab_]bYZ`XaYaaBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:10039:17071#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGGGTTGTGGTG ++ +hhhdHcdddeeeehhhhhhhhhhhgfhhhhhhhhhghhhgehhhfhhhggghhhhghhghggfhegdhghhfgdcbeahdgdcffffbdaagdcccacbae[aaddaba]dccccdccaaa_aQ][Z^\`^Z]_\_aBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:12821:17733#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAATCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGAGTGTCGGAGGGGTAAGCGGAAGTCCTAGTG ++ +gggeGdeeacddcggggggcgggggeggggcggffbfbcacffgggggcgff]fe_fffc]]ac_c]ccfc_abbefd^`\^\aaac_aa_afb`W``aaa[a_ca\]]]`]]acK_\\W\N]_d_aa]ST_BBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:6118:18575#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGGATTCGTGGGG ++ +dffbFbbaUZZ^Zgggggg_gggggg[gggggggggagggfgggggfgafffafd^bbff_fffed]f^`dbdcccf_d_b^dfffddgfgcbceeeeggeWgf]cW[cccf[X^W`_accaceeaac]`b]]c_`a`BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:5450:18847#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTTGGGGGG ++ +hhh`Hbbb_aaaahhhhhhhhhhhfhhhhdhhhhhhhhhhhgghghfhhhfhhhhghhhhghhhghhfehhghfgcfdhgggggfhhgggdg_addbdceedecggdg_dedcacaaa^[[WSPZZWW[X[XX^ZW^^BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:2535:18871#0/1 +TACGNAGGATACGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCAGGTGGTCTTGCAAGTCAGTGGTGAAAAGCTGAGGCTCAACCTCAGCCTTGCCGTTGAAACTGTAAGACTTGAGAGTACATGATGTGGGCGGAATGCGTCGTG ++ +hhhaHdbacddcchfhhhhchhhhhahhhhhhhhhhfhhhdhhhhhghhhhhghhhedhggghhhfafdfhhf^_^ccdabbdhcghheghhh]fcfchhdeaccfcW`cffcb^K[^bbb]d_bW`R^d`R`fccaBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:4582:18950#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGTG ++ +hhhbH]b`^``^`ghhhfhhhfhhhdhhhfhhhhhfhhgffggffgfhdffeggdghgfgefdfcffgadddeaaad_dddddadadacc`dcdccddaa`cd_ad_aUWQUT``_\]_a_\\\__\_ZZ^BBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:15559:19608#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGGTTTGGGGGG ++ +hfhaG_aacdddchhhhhhhhhhhfghhhhhhhhhhhhgghhhhghhhgghghhhhhhhhhhghgfghhhhfhdechdhegdefcfff_abebgbdaeada_dadd_e_]a`cWXX[P[[WVVNXJOVZTYTS_\Y__BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:9787:19631#0/1 +TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGACGGCACTGCAAGTCTGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTGGAAACTGTAGAGCTAGAGTGCTGGAGAGGCAAGCGGGATTCCTAGTG ++ +gggeGeeea`ab`ggggggggggggggggggggfggggggcgggggggfggggddggggeggggfgdgfdgdgdddaadaaaaaV_b_aaaLa\__\O]_]\GZUOWO\S]\S\U\ZZ_\_^_\aBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:17851:2016#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGTTTCGTGGG ++ +hhhccdcFcdddchhhhhhghhhhhghhhfhhhhhghhhhhhhfghhhgghhhhggghfhegfhgb]hdhhdhac]daWTTNEXWWTS_^`b^_abbdddcadcddRd_adbbcacaaadaaa^^\^`__a\\^^V^^\^^BBBBBBBBBBB +@HWI-EAS440_0386:1:24:12302:2593#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGGNGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGCGGTATTTGTGGTG ++ +aaa\\a_DRZV^Zcccc_cccccccccccccccccc]cccccccIcccZ]cRZ``VMZ^a]a]cccccbcccccaac_WJQLDVVTLK[[[[SLYTYYccccbccc`cZV[][PKSUW[ZXZ[X_BBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:8488:2754#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTTAAATTGCAGTTGGTACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGATTGCGGTG ++ +]YZZZ_^DQZUSV^c]accccccccccccccccccccccccccccccccccccccYLcaccccc[POccccccaccQ[UMQGD^XZWTaaL]UIFUHT[]^]^NFFFSY\\XWH[ZJTcc]ccccbcBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:6114:3818#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATAGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGGTTTGTGGGG ++ +hhhddacF^bb`ahhhhhhhhhhhfghhhhhhhhhghhggghhhghghgghghgghhhhhhhghgffhhhhdccdafc]\\[H^\][[ccaedefcdfffdbfccdcdaabdbacda`_b^_a`aa]caa[]__Y[aBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:13084:4634#0/1 +TACGTATNGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGNGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGGGTGTCGGGGGGGGAAGGTGTATTCCTATGG ++ +hhhdaacFa[a^ahhhhhhhhhhhhghhhhhhhghghgcccffhghhgfghhgghhhhhhhggdaeffdhedebcdecZZZ^FZVYVY_^`Lb`^\\[daaaaddb_b^Q^]^X[^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:9705:4722#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGGCGGGAGTCGTGGGG ++ +ghheedeFacdacghhhhhfhhhhhahhhhhhhhhfhhgggghgghhhgegehhg_hcggehggfgbhfggfhcddhd]]^^H\^\\\dadebddddccfdefaadcdbabaadab]_]^WY^BBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:6985:4749#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGCTTCGGGGGG ++ +gggaa^aEZ```_fffffgagggggggggcgggggggggegggggfgggfgggggegggfggggdggfefeeade_gd[[ZYF\Z[ZZdd]dcgacgddcdcedgeadaacacWca_`ca_aaadd_aaa]aBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:2227:6089#0/1 +TACGTAGNGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCAAGCAACTCAGAAGTGAAAGGCTGGNGCTCAACCCCGGGACTGCTTTTGAAACTGTTTGACTAGAGTGCTGGAGAGGTAAGCGGAATTCCTAGTG ++ +gggdaacGdcccaggggggggggggdgggggggggfgfgegggggggggfgeggegggdfeddK]_^^bdeaddcfce\Z\`F\XX\ZcddadddQaaaaccaaaa___aba]b\_]_b_W]a_baaBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:7654:6279#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGAGTTGTGGGGG ++ +gggdeaeF^`b``geggegggggggfgggggggggfgggggffgfgggfbgdggdffgfgbfffcfbgdgdacaaada\][^G\[W[[a_[daaaabbccccd`cd`_a^`aaaa__W_a]]_^]___BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:11532:6376#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGATGCCTTGAGTGCAGTTGAGGCAGGGGGGGTGTGGGGGG ++ +gggddccF`bbbaggggggggggggdgggcgggfggggggggggggggfefefgggcgggggggggggggggfecbfe]]]]F\[Z[Zddcfeaeddedbddedggcfeabcbdbeaa`c`\_JZSWZ]__BBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:6005:6667#0/1 +TACGTAGNTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGNGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGA ++ +hhhedeeHcdddchhhhhhghhhghhghhhhhhhchhhhhhhahghhhhhhehfhhhghhhhhhfhghhggegddghh`a`dFa][^^dgcdggbeegdaacdedcVa`]a^`cgWd]dd[d]ddb_a_][`PZ__^^Wa_\a_ZYaBBBBB +@HWI-EAS440_0386:1:24:9083:6728#0/1 +TACGGAANGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTAGGCGGTTGGTTAAGCGTGTTGTGAAATGTAGANGCTCAACATCTGACTTGCAGCGCGAACTGGCTGACTTGAGTACACACAACGTAGGCGGAATTCATGGTG ++ 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+AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCTCGGGG ++ +ffffffffgggggggggggggggggggggggggggggggggggggggggegaggdbbgacgedg`d__aca\a__`QcGTTTRc````d_dd`]ZHXZ\\`]Va_\_VWZYWQ^b[][X^[]]]XW\_BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4380:1628#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGCGGGGATTTGTGTG ++ +hhhfhhhhfhhhhhfhhghchghgge_hhehhhhhfhhhghgfgahhhfggfhedddegggcddcf]cadeaca_aa\ddaaadfdcca`]c____abaaZac__]Y__]a_^\P___R[XVUV\U]ZZ^BBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:1510:1668#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGTAGGGCGGGGTTGTGGGG ++ 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+TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTAGAGTGTCGGGGGGGTAAGTGGAATTGCTAGTG ++ +aaacccccccccccccc_cccccccccccccccccccccc_ccccccccaccccc]cK\YM[\cabacccccccacaaccbcccQccc_c_cccaY_cBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:7669:2080#0/1 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCGACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGGGATGTATGGTG ++ +hhhhhhhhhhhhgffhhghhhhhhhghhhghhhhhfhgghhhhgchhhgggchhhghhgcgfdgdgfcfgdcfcgddedgfKdcbcbe_daadba__aU]_`_dbaabaadd`]aaa__ca`a\_W__BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:7091:2092#0/1 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAAACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAAATGTAGGACTAGAGTGCAGGAGAGGGAAGTGGTATTCCCAGTG ++ +hhhhghhhhhhhghhhhghhghhhhgfhhhfhhhhdgdedefefhghhfhgaghgfgghhfffeaeheeddadddd]d_b____\Z_\\^\\_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3922:2110#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGGATTCGGGGTG ++ +fffggggggggggggggggggggggggggggggfggggfggggfagggggggggeggggdggggdedgeffef``]b\ffcacdcddabaaddaa]ac_aa_caaaa\b]abba]daaW^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3806:2202#0/1 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATCCCCGGTGG ++ +gggggggggggggggggggggggggggggggggfafffdffff_ffcfggefggggggffedfafffRbd]d``afcaeedced]d]cba_deeab]dcaccJb``]]X^\XXcbb^`b`adaada[aac_a___aBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:6540:2228#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTAGAGTGTCGGAGGGGGAAGTGGAATTCCTAGTG ++ +hhhhhhhhghhhhhhhhhhhhhhghhhhhhhhhhhghhfhhfhhhhghhhhcfhaegcgadgghdcgfggheeffg_]ddbdafcdbcddddddcWa]___^^ddaIaVPZQ]V_^\\\_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:10019:2223#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTTGGGGGG ++ +hhhhhhhhhhhhfhhhhhhghhhhhghhhhhhhhhhhhhhhhhhghfhhecghhhhhhhhhhghfgghghhdhedchchfhdgffddfddfefdaccedcd_eade_ca\aacaaa__``^R^BBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:2668:2254#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGAGTTCGCGGGG ++ 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+TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGGGGGAATTTGGGGTG ++ +hhhhhhhghhhhhhhhhghhhhhfhehhhhhhhhhghhhgghggehhhfhhhdfheghhfghhhfgfhfhfghdcae`dggedffcfd[baebfcdffffdfgddbd]bab_aca_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:8482:2380#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGCGGGATTCGGGGTG ++ +gggggggggggggggggggggggggcgggggggfgggggggggfefffeffdffffffgggggfgggggggggdddfbgggfffgggagfg]ddcacdgggceaef][^^b`bWcc`acddadca_ada_a]aBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:15658:2382#0/1 +TACGGAGNGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGGTGCGTAGGTGGATTATCAAGTCTCTTGTGAAATCTAATAGCTTAACTATTAATTTGCTTGGGAAATTGATAGTTTAGAGGAGGGGGGGGGCAGATGTAACTCTTGGGG ++ 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+TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGGATTGGGGGTG ++ +hhhhhhhhhhhhhhhhhghfhhhhhfhhhghhhhhfhgggggghghghcdhfbhegghgdhgdgdgdhdhgdhacaeaebcccfdfddcccfbacaac`adadcfcd`dababaadaa_daVaa_aaaU^BBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:13134:2667#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGCGCGGCTGGCGGGCAGGAGAGGTACGTGGAATTCCTCGTG ++ +hhhhhhhhhhdhfhhhhhhhhhhghhhhhhhhhfhdhghhhhhgghhhhhhgfggggheffddhaghhadgdefffafdfdfafcdacd`aadaa\ZRRYTMY__^U^\O\^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:11175:2690#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAGGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTCGGGGGG ++ +bbbfafffdfffffffdffffffffcfffffdcffeffffffffeccfeffdfffadfdfcfffef]feffefeddfdede_ddddedadcecbc_aadcd]dcbeadcade]Z`]Y]``W]]Z``BBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:8576:2709#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGATGGTGGGG ++ +hhhhhhhhhhhhehghhhhfhhhhhehhhghhhhhhhhhhchghhhghfhhghhhhhhhhegghfgfhghfdcaaafafgegfbfgfdaddhddaddbddgbgadeaaa_aaab]d_a^__^_\__aaZ]_^]_^]`BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:6943:2731#0/1 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGGG ++ +fffgggggggggggggggggggggggggggggegfggggggggggggggggggggfgggfggegggggfggdgcddcd_bddacegddfagafb_dfbddddbfdffaaddad``da```dWda``]bT\_[]__BBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:1850:2741#0/1 +AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCGGAATTCACGGTG ++ +cffdaddd^aKa^fffffffffffffffff]ffcfffffffffffffffffffff`]]f_]f[d^add_ffcffcfacfda]ccfcfeefcWeaa^^]fcfca`_`cZ```dT^aZZS`ccaaa_a_cPM^W^^LWYYZ^BBBBBBBBBBBB +@HWI-EAS440_0386:1:25:10428:2744#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTCGGTGTG ++ +hhhhhhhhhhhhggghhhhhhhhghahhhehhhhhghhhhhhdehhehdgegghhehhhhghghdhghghhehbdbhagghedgcdfdbaagdcbacebddagaadb]a\aaabdac`_aa]a]aa_ba\^_BBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:12398:2774#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTTGGGGGG ++ +hhhhhhhhghhhchhhhhhhhhhhhehhhghhhhhghhhhghhhhhhhhhhhhhdhhhhhhhfhggdhghgghbdghgghh_dgdgedfcfgfdeddeffadgadd[e__acccbacaccaaa]aaadada__]_\_aBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:14110:2805#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGCGTGTCGGAGGGGTAAGTGGAATTCCTAGTG ++ +hhhhghghdfffdhhhhhhhhhhhhfhhhghhhdfafccccfffhhhadghhegghhehhfcab_]dbR_^]_fdff__cac_dWbb\aacad`bKRW\\YZ\_^KR\XL[Z[^`^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:14127:2819#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGGTTCGGGTGG ++ +ffffffffgggggggggggggggfgggggggggfgggggggggfdggggggggggfggggggfdaddfdffefbbaf_ddecdddfcddaagdcdaddffadfa`dac_`gcb^bd_^acaY]UW[\\\aBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:1968:2841#0/1 +TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTGGTGAAAGATCGAAGCTTAACTTCGGTAAGCCATGGAAACCGTAGGGCTAGAGTGTGTGAGAGGATCGTGGAATTCGATGTGT ++ +ggggggggdffffggggggggggggegggggggcaffeggggaffcccggggffggaefebffeadffcfc]c]X`b`bdddaddaabca_de]caa_bRI\_``_\_a`ddabba_bBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:10866:2839#0/1 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGAGTAATGGAGGGGGAACCGGAAGTCTCGGTG ++ +hhhhhhhhhhhhhfhhhhhhghhhhhhghghhhhgfhhhhhhdhghhhgghhhhhhhdhhegdfcdfahehcefffafcfddbd__dda^d[]^_`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:11767:2838#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACCGTAGAGGTGGGGGGGATTCGGGGGG ++ +hhhhhhhhehhhhhhhhhhhghhhgghhhghhghhghhheghheghhhgeghhcgghghhhhghdgchfhgfeccbeba_cdaedebeb]dccaaaaa```^`_aa_d_^]L_`_a^^_caL`BBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4402:2852#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCGACCGTAAAATTGCAGTTGATACTGGCTGTCTTGGGGACAGGAGGGGTGGGGGGGAATTGTGGGG ++ +hhhhhhhhhhhhhhhhhhhhhhhhhghhhhhhhhhhhhhhghhfghhhegghghfhhhhghhhhfgghghfghdbbfbeeheegcefa\__caaaaaaca^_daaa\a]\a_aaaaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:1421:2878#0/1 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGGCGGGACATGATACTTCGAGACTAGAGTAATGGGGGGGGGACCGGGGTTTTCGGGT ++ +_ffK[b]`fffffggggggggfggfgggggggggggggggggffffffgggggggggggggggffffffffdf[ccfaafafd`Q]b_cKcccBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:11319:2883#0/1 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTG ++ +ghhhhhhhhhhhhchhhghhhhhhhahhhfhhhhhfhfhghehgghhhfghhhegghfcehddhdehceggfdfagbegfdfcfbcdabbaaddb]a_a]aW_aaaYaZ^^^X_a]b_]Z^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3648:2910#0/1 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCGCATGATACTGCGAGACTAGGGTAATGGAGGGGGCACCGGAATTCTGGGGG ++ +hhhaffffhhhhhhhhhehhhghhhhhhhhhhhhchhhfffffhhhghhhhhhhhhhhhhhgefdffdfdfefeebaedgggagcadbdbgddaacVa^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:6586:2930#0/1 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGGGGAAGCGGAAGTCCGAGGG ++ +hhhhhhhhhghhhghhhhhhhhhhhhhhhhhhhhhhhghggghhghghdhhedgghhhhhhhghfhghacgdgfgggfggdfcabce]ccbaed_^aa_a_VWaaZ_\__^_\Z`\^^^^_Y]\_\aBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:8471:2931#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATAACTTGAGTGCAGTTGAGGCAGGGGGGGTTTGTGGGG ++ +hhhhhhhhhhhhhhhhhhhhhhhhhfhhhghhhhhghhggghhhghhhddhehhheghhhggdhfffhfhhfhffchdffgfgcdd`abbdcab_aadaacaeddf`aa_abb[[^FJ\][[ZY`O^`BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:14208:2940#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGGG ++ +hhhhfhhhhhhhhffhhhhhhhhhhhhhhhhhhchhhhhhhhghggghhhgehhgghfhhhhgfdhcfehhghgedhdgfhfgffdf_ddafddacdddcfdggcfggfccbfacaa^cac_a^^`W`^_c[aVL^BBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:2027:2951#0/1 +TACGGAGGATCCGAGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAACTCGTGGTG ++ +fffffffffffffcfcffgggggggaggggggggggggggggggfgggddf_cgggggcggggfdgaggegdgdc[fceefbeddbdd]a_fadaaaadd]be]```BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:13175:2941#0/1 +TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATAGCAAGTCAGATGTGAAAACTATGGGCTCAACCTGTAGATTGCATTTGAAACTGTTGTTCTTGAGTGAAGTAGAGGTAAGCGGAATTCCTAGTG ++ +hhhhhhhhhhhhhhhhhhhhhhhghhchhhhhhfhehehhhhhhhhhhghhhhhhhhgghhhghfhghggfghgffgcggggbefdffdabgeadeacddcaeddddaaaedadd_d_caa_aX\ZZ\^_`\`_\^``Za]WZW[\R\\_BB +@HWI-EAS440_0386:1:25:13489:2951#0/1 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGGTACTGCGAGACTAGGGTAATGGAGGGGGAACCGGGATTTTCGGGG ++ +hhhhhhhhhhhhhghhhhhhhhhghhhhghhhhghfhhhhhhchhhhhhddfhhghhhhhhgffdffdfcdbdggdgbKUW\\`ZU__aaaaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:6132:2959#0/1 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCAGGGCTCAACCCCGGGACTGCTTTTGAAACTGCAGGGCTAGAGTGCAGGAGGGGCAAGTGGAATTACTAGTG ++ +`]afffffffcffffffff_fffffffffffffffffffffffcffcfbee]^ffffffdffffffWffffcffaf[fdf[ffdcdffffddfP\^^adec]bd]bdbcdadb^]KJ^`\^^\\a_aa`[]]N\X^W^]]^BBBBBBBBBBB +@HWI-EAS440_0386:1:25:2692:2971#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGTG ++ +hhhhhhhhhhhhhehhhhhfhhhhhahhhehhhfhehhhgggghghhheghfdhhgghgghg]hehdhggfghab]fbedhddgfdfcddcfcabaaaccddg_[aaadabaddeddaddd_`Y^_aa[TWFQ[[[UXZ^`BBBBBBBBBBB +@HWI-EAS440_0386:1:25:1831:2983#0/1 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAGTGCCGGAGAGGTAAGCGGAATTTCTAGGG ++ +deeffffffacffggggggggggggfggggfgggggggggggggggfgafdffdf_ffffdeefdbffbbd`bcccWfZ_`___accaedgdca]Q_`dda]a``]]X]\``VV]]_^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:6335:2989#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGCTGAAACTGGCAGTCTTGAGTACAGTAGAGGGGGGTGGAATTCGTGGGG ++ +addfffffRdadcdddddffR[fddc[dff_dcffa[fdddd`aMSaaT]_]Kdd[ddffffcfefff]ff`ca^^[PUNSTXcecfeeecaPTIXYN`````G^^ZZebbaccc_]aacaaaWT]Z`BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:10162:3028#0/1 +TACGGAGGGTGCAAGCGATAATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGGGTGAAATCCCACAGCCCAACTGTGGAACTGCCTTTGATACTGCCAGGCTTGAGTACCGGAGAGGGTGGCGGAATTCCAGGTG ++ +aaafffffccfff`b`_KZ[TWZfcffffffeffaffdffffffffdfdfdfafdfffdfWddededfeae`cbbdacaeadddd`daaacc[]`W]]`ddad_caaWca_aO]XH]]_\W_]YRYW_BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4348:3042#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGTGTGGGGGG ++ +[_affffffffffffffffffffffcfffffddfffffffffffffffffdffffffadfffffdffffffffafQfafcffcdfcfedfefdfeecddfcdfdecdfccacebWebaccbbccaeabT``]`]`[``BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:5972:3048#0/1 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTG ++ +hhhhhghhhhhhhhhhhhhhfhhhhhghhhh_hhhchhhhhhhdghghghhghhhhheghhgfhfgdhhheghfgfcgggbfdbbcdfgcdcfdaaadcfcafbbb^`_\a__Wa_aBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:9861:3050#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGGTTGTGGGG ++ +fffggggggggggggggggggggggggggggggggggggggggggggggggggfggggggggfggggggggdgbc_fcgaggggdffdd`bd_dbdbbaca[c\c__`]W````T_WX^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:10090:3050#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGATTGTGGGG ++ +hhhhhhhhfhhhgghhhhhhhhhhhdhhhdhhhhhghhgggggggghhfgdghhhghgeghbfgdedhdhgdg_\ac_cdedbaYaaababebaab\bbb_Wd_baabaaaaacWc_Z``_^^accdfca``c\]___BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3139:3077#0/1 +TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTGGAGCGGCAATCGGAATTCCGTGGG ++ +ggggggggcffffgggggggggggggggggfgggggggggggggfgggedggggdgggggggggggcggggffgfgfcgggefffggggggegfgfgfbddQda[`c_dddaadf`fdbdaaWP^Z^^BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:9110:3081#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGTGGTTGGGGGG ++ +hhhhhhhhhhhhhfhhhhhhhhghfghhhghhhhhghhfghhhhghhhghhfhhhghghhhgghfhghghhfhcbahdeggegcfgfdcadfbdcbcdbaaad_cc_a`]`^ba_a__acaaa\a_\aaaaaacVaaaBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:17734:3100#0/1 +TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGT ++ +ffffffffcffffggggggeggggggggggggggggggggggggfgdggggggggdgedeaffgffggadfdfegadggagfggcgggfgfagbW]W^dbcddddb[abdda_XPZ\\aaYa_aa]a_NYYYZT\\]]`BBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:8423:3141#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGCGGATTTGTGGTG ++ +hhhhhhhhhhhhhhhhhhhghhhhgghhhhhhhhhghhhhefggghfhfgghhhhhhghhgghhfeehgggfhbgfgfgffgdcggfceffffffca_abbac`ad_d_aaaaddae_^`]^\\a_N\^_^]Z_aa_BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:14009:3149#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGGGGAATTCGTGGTG ++ +hhhhhhhhhhhhhfghhhhchhhhhfhhhehhhfhhhhhhhhbffhheacff_heehegfghgcadbdgfgfeecWebfbffffefdacdddaddfdfaa]aa_aabb`\Z^^\[^ZS]`_\^Y]_^^Y__G_BBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3100:3158#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGAGTGGGGGG ++ +hhhhhhhhhhhhhhhhhhhchhhhhgfhhhhhhhhfhhhehghhghhhfhgehhghhghfgffgdg[gfgfdeab]dadddebfdfcaddbdaabb[_acaadcacac_ababccc`ca_\\_^_a\__aRa\__BBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4738:3152#0/1 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGGATAATGGAGGGGGAACCGGAAGTCTCGGTG ++ +gggaffffdgggggggggggggggfgggggggggggggffffffffffgcfggggggggggggfdffdfdfdfgcg]ggfbeffaccddabdb`Qb]_[^\]`a__]b_a]_bBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4197:3165#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAGGTAGGCGGAAGTCGTGGTG ++ +hhhhhhghhhhhgfhhhhhhhhhhgchhhhhhhhhhhhghhhfhhghhhhghhfff`ffcffchhhhhfhhhhfhhhbhggheghhhhchfhf_adddaeedeeeeadgddffcccf\aaaaa`gcda`_`\W^X\R^BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:9072:3171#0/1 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGGAATGTAGGGGG ++ +ggggggggggcgeggggggggggggfgggfggggggggggggggggggffff]gggggdcfccfefffcffafddfaffWf_cdffdeded[ffddedffgfRcfaaaccfc_fecddcacaa]aaacaac_]^YZ^^\^aBBBBBBBBBBB +@HWI-EAS440_0386:1:25:15751:3166#0/1 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGGATTCACGGTG ++ +gggffffffffffgggggggggggggggggggggggggggggggcggggggggffPc[^`Z\Zfefaff[fcfccffa``cf_ggagefgdfd[^\WWccc`c__aaQYZ^_^a]]cZ`X`WbBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3473:3225#0/1 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGGAATGTATGGGG ++ +gggg_ggggggggggggggfggggggggggggggggggggggggfgggfggggggggggggfdgggegdfdcfcccffeeecc`adddccbefddcab`daddddd_daadaaddddWa__Y[W\\[\a``]`_Y_W_BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4755:3229#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTCGCGGTG ++ +hhhhhhhhhhhhhhghhhhhhhhhhghhhhhhhhhhhghgehhghhghggghhhgghgghhhghfhgheghegdcahceedggffggdedahdeebddc]dcdaddcaddgcadedacfddccaedcdabaaaZbaaaBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3032:3237#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAGGTAGGCGGCATTATGGGTG ++ +hhhhhhhhhhhhhghhhhhhhhhhhghhhehhhghghhgghgghhhhhgehhfhec`fhhgeggdg_hghhfhgffhdgfgdgfgegfdddhggddd_ddddhdegadcefceffddcddaaPW`___a_WW_BBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:1630:3249#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGGGGTAAGTGGAATTTCTTGCG ++ +hhhhhhhhhhhhhhhhhhhhhhhhhghhhhhhhhhhhghfhhfhhhhfhhhfhhhghhhhhggghhhghhhghggh[gehegdhgdgffgbagaf[ccc`^_`c_W\a``^[U^``^^P[[XZQTSU[^JY_BBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4476:3251#0/1 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGAGTAATGGAGGGGGCACCGGAACTCTCGGTG ++ +hhh]ffffhhdhhhhhhhhghhhhhhhhhhhhhhahhhhhhhhhefhhgghhhghhghhfeggfdffcfbfdfdfeddgeggf_H_^^`Z`\ZYZ[I\Y\[\^^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3312:3259#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGAATTTGGGGGG ++ +hhhhhhhYcffffefhhhhghhhhgehhhchhhhhghhhgchhhbhhhdghghhhghhhghhgedd]hhggfhfd]edddegddffffbgdgffdfcddff_fccdcfdadfcad[dadaaab___BBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3550:3291#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTTGGGGGG ++ +hhhhhhhhhghhchhhhhhdhhhhgeghhhghhhhgehhhghhhhhhhdgfhchhchfheeahhgdchdddefdgdhaebecdfgfffgfgfd]ddcdadaaccbbccb]cbb_b^babd_aaaa]X_[]^Z_JZ[^BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:5478:3298#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTGGAGGTGGGGTGGAATTGGGGGG ++ +hhhhhhhhhhhhhhhhhhhhhhhhgghhhghhhhhghhhhhgggghhhgghhfhghhhgghhhgbhghegheedd_gcgfgefffffdfddgdfgdbfddffgaaccaaWa_a__`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:15346:3296#0/1 +TACGGAGGATCCGAGCGTTATCTGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGGTTCGTGGTG ++ +hhhhhhhhhhhhghhhhhhhhhQffcffhghhhhghhhhghghhdhhhhehhhhhghhhhhhfheechhhhghddchdhdeggadhbdacbdadbcdddgdaeabdaca\aac^``^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3468:3322#0/1 +TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGCTGTGCAAGACAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGTGACTGCACGGCTAGAGTTTGTCAGAGGAGGGTGGAATTCCGCGTG ++ +hhhhghhhfffffhghhhhgahhhghhhhhhhhhfhhgggghhhfhhchghhhhgghedhdddecbeefdfcgffdffffgfddcffddccfeWdaabcddcaad___ababaa_daaaa_\\S_^^R\\\XX_Pa_BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4199:3321#0/1 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCATCACAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTTGAAACTGTGGAGCTGGAGTGCAGGAGAGGCAAGTGGAATTCCTAGTG ++ +hhhhhhhhhfhhhfhhhhhhhhhhgchhhhhhhhhghhffdffhghhhhhghdhhhdhhhgfhhghhfhfhdhgghgggfdfdb[dgbffgded[da_aad]bbda]aZN\^\ababa]aa]__a[_^[WTIXQWTVW\_aBBBBBBBBBBB +@HWI-EAS440_0386:1:25:8568:3330#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTCGTGGGG ++ +fffgfgggggggfggggggegggggeggggggggggggggggggdgggddfcfgggggggggggfggggggggcffgfggggedgffagadgdfgfgffgfcg`dgdgaacaaaab]adddaa`d``ddad`a][_aBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:12050:3335#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGAGTTGTGGGG ++ +ggggggggdggggggggggfgggggggggcggggggggggggggggggggcggggdeggggg_gggggdggdfdddgcgdegdfgd_dgffgfb_aWcdddbdaddcWZ^\^^cabcabbbacaa[aaabdWdR\R\^BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:19075:3342#0/1 +TACGTAGNGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTGGAAACTGTCGTACTTGAGTATCGGAGAGGTAAGTGGAATTCCTGGTG ++ +hhhdeeeHdcccdhhhhhhhhhhhhhhhhhhhhhhhhhhhhhghhhghhfhghhhhhhfhhddhbefhdfhcfdeaedaebedd__baa]bab_a[G`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4994:3354#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGCGGGATTTGTGGTG ++ +fffgggggggggggggggggggggggggggggggggggggggfeggggffgeggggggggggggfgfgdggdgc]bdbffgg_ffbeffdfgfa_aaaddddg_aabba_a]bd[b_aadaa`cd``]\`__\Y\\^BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:12091:3381#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGGGTAAGCGGAATTCCTAGTG ++ +dddffffffffffffffffffffffffffffffffffffffffffffffffcffffffffffefeffffcfeffe]dfdfdedcWcfcdddafcfacecacacdd[adc__`_WaZ\X]W]J]ZZSXZ^BBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:8594:3395#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTTGTGGTG ++ +hhhhhghchhhhhchhhhhhhhhhfchhhdhhhhhhhheeehhhghhhgdhhhhfehhhfhhghdgdhechehfffgdhdcgbfgfggcddhddddddfff]fcdcaa]`c`c_`Z]S[\[XUVY[[\Y^BBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:2052:3400#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGGACTAGAGGGCAGGAGAGGTAAGTGGCATTCCTCGGG ++ +ggggggggggggggcgggggggggggfggggggggdggffafffggggggggggfggggggfdfeefeefecedegecgddgabQadbW\^^`aaa_c__]aaaa_T_VV\\Pbb_aaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:11776:3405#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTTGTGGGG ++ +fffhhhhhhhhhhhghhhhfhhhhhchhhfhhhhhhhghhhfhhfhhgdfffbhhehhhghhhhgfghgghdggdccgeahceeggedfffgf]ddadabdab_aaaa\UX\KYPWUYdadabZZ_Y_BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4155:3413#0/1 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGAGTAATGGAGGGGGGACCGGAATTCTCGGTG ++ +gfgaedeeeffffcffffggggggggggggggggfgggbbbdffffffgggggggeggggggffcffafcc_fedddbbfgbfaO___\W^^[QW^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:11052:3431#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTTGGGGTG ++ +hhhhhhhhhhhhfhhhhghhhhhhgghhhhhhhhhghhgghhhhghhhgfgghhhgghghhhghdddhghhfhebchaggh_ggfdcc]a_cadaaadddadebdfdda[caaddada_aa[b\aV_a_ba_BBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4122:3435#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTAGAGTGCCGGAGGGGTAAGTGGAATTCCTAGGG ++ +S[_]cccaccccccccccccccccccccccaccccccccccccccccccccccccc[accccccccccccccccccc[ccc]cc[cbcccc[cSWR[W]][]SaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:9796:3444#0/1 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAAGTGAAAATCCGGGGCTCAACCCCGGAACTGCTTTTGAAACTGTGAGGCTAGAGTGCAGGAGGGGTGAGTGGAATTCCTAGTG ++ +hhhhhhhhhhhhhhhhhhhhhhhhhchhhhhhhghghhfhhfhhghhhhhhgghahhhhhhhfhdhgfhhhdghh_hfhgecghfchggeddhfaa[cceeeedecdaacffaebddbccc_c_eaaccZaa``_cXcYY^R\[UTWTTZY[ +@HWI-EAS440_0386:1:25:2235:3462#0/1 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAGTCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTG ++ +gggfdfffefaffggggggebggggggggggggggcgdeeef_ddde[geggggcgeWcgaggg`fgfeec`cccebaddcc`bdadadaV]d]]H]_dcbb_a[]YTYUY]UW]O`ZX]ZY_^[]^^_aBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:14529:3458#0/1 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGCCGGCACATGATACTGCGAGACTAGGGGAATGGCGGGGGAACCGGAATTCTCGGTG ++ +hhhhhhhhghhhghhhhhhegfhhfhfhahghhhchhdhhhhahhhhhggchffgggghhggfhbefbfbd_dddcdaR`_`a_NU^^^^__^`^`G^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:6708:3509#0/1 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTG ++ +fffgggggggfggggggggfgggggfggggggggggggggggfgggggggggggfagggggagggggggegfgaggefdfddadgggccca_fa\^\^ddd]dacR\_``accc]aab`[`\`BBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:13919:3507#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGGATTCGTGGGG ++ +hhhhhghhehhhffghhghchghhfahhhdhhhggdhhgdfgggdgfhcadfahedeeddgdagdebgffgdgd_agaeceddfdccdaaagd_caacadaadcddcad]`dbcbca_aa_Yb_aa_a\O\O]^\]_BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:10705:3535#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAACTGGCCGGCTAGGGTGTCCGGGGGGGACAGGGGATTTCCTGTGT ++ +hhhhhhhhdhhhhhhhhhhhhhhhhehhhhhhhffdfeffffchhhhhhghhhhhhhhfhhhfhdhchhfheagghhcahgeg_ddbadeedh`][^`Z_ZZ```RK^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:7788:3570#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAAGTGAAATGCGGGGGCTCAACCCCTGAACTGCTTTGGAAACTGTCATGCTAGAGTGCAGGAGGGGTGAGCGGAATTCCTAGTG ++ +hhhhhhhghhhhghhhhhhhhhhhhhhhhhhhhghhhhhhghehhhhhgghehhhgfgghghhhgghheehbhaWacfc_ca`bedcfbddaeeebaaadada``ZW^]^```aabb_aa]]b_ca_a_V___WRZW_WX[VJ\W\G\T^X^ +@HWI-EAS440_0386:1:25:2273:3577#0/1 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGACTTCCCGGTG ++ +cfffeffeggaggffffdggdgggggaggggggee]fb`]```fcfgdgaffgffbbd[ca_W]Z``ZY^]U]YS[^`_`_`\`add```aa]a_[[a`a_a```a`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:14850:3657#0/1 +TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTAATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTGTGGTTGAGGTAGGCGGAATGCGTGGTG ++ +[__fffffffaffffffffffffffffffffcdfffffffffffaffffffcfffa[affffffffff][fffcfcfffdfcfffffdfed[cfccccfffafccfcYaaccc^^Y[^ffcaaZaBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:11581:3662#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGGGTAAGCGGAATTCCTAGGG ++ +hhhfhhhhfghhffhhhhhghhhhghghhhghhhhdhdheefffhehghhgehghgcehhhfdgdgghghffedff[gdfac]_]`c`ddd_c^`\[\_a___a_a]aaaab`__H]_[S]P]__]Z]`SY_`BBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:16965:3660#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAGTTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTTGTGGGG ++ +hhhhhhhhhhhhhhhhhhhghhhhhchhhhhhhhhghhghhhhhghhhgchehhhhhhhhhhghfhfhgghdheedhdhghehffgfgcgggfgfa[ddcgdeffgafdcgggabadaddc_aW___`_a___W^R^BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:8197:3691#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGAGTGTCGGAGGGGTAAGTGGAATTCCTAGCG ++ +hhhhhhhhhhhhghhhhhhhhhhhgfhhhghhhhhhhhhgghhhhhfehhhhhhhhhhhhhgfheefhehhehfgahgfhghfgffdccacaec]a__aaa\aaca^_aYaaadddba^^`S_S^\\^BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:11617:3701#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGTG ++ +\a_fcfffdfdffffffffdfffffcffffdddfffafffffffcfffffff_ddaad^^^cafffdfaffcffffab^O^[aefe]acfccfbaccccccccdffeccc`fJGQYRXca```BBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:15484:3705#0/1 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCGGTGAAATTTTGGTGCTTAACACCAAACGTGCCGTTGATACTGCTGGGCTAGAGAGTAGTTGCGGTAGGCGGAATGGATGGTG ++ +hhhhhhhhghhhhhhhhhhfhhhheehhhehhhhhghhhgdhhhghhhgghehhhhghhhggghfggeghgdhcaaefgegedgdhhdacaddcbcde\caccbac\c_acdacebb]a_aSa\_\]_\aa]W^BBBBBBBBBBBBBBBBBB
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/pick_open_reference_otus_otu_map.txt Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,10 @@ +708680 L1S76_81 L1S140_70 L1S8_6 L1S76_31 +851865 L1S8_28 L1S8_76 L1S281_24 L1S105_1 +589071 L1S140_45 L1S208_48 L1S105_50 L1S257_5 L1S257_27 L1S257_54 L1S281_2 L1S105_16 L1S140_47 L1S57_30 L1S105_34 +531539 L1S208_60 L1S208_83 +183480 L1S208_7 L1S281_19 L1S208_57 L1S257_55 L1S281_8 +583117 L1S76_13 L1S257_63 L1S105_35 L1S8_84 L1S140_9 +589277 L1S208_15 L1S257_22 L1S140_67 L1S140_69 L1S57_52 L1S57_62 L1S281_64 L1S140_26 L1S208_58 L1S208_14 L1S140_32 L1S208_3 L1S281_12 L1S57_68 +580629 L1S105_71 L1S76_74 L1S76_61 L1S8_38 L1S105_29 +535375 L1S8_4 L1S8_23 L1S76_21 L1S8_53 L1S57_41 L1S281_78 L1S76_25 L1S76_73 L1S281_43 +363400 L1S57_77 L1S57_42
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/pick_open_reference_otus_otu_table.txt Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,12 @@ +# Constructed from biom file +#OTU ID L1S76 L1S140 L1S8 L1S281 L1S105 L1S208 L1S257 L1S57 taxonomy +708680 2.0 1.0 1.0 0.0 0.0 0.0 0.0 0.0 +851865 0.0 0.0 2.0 1.0 1.0 0.0 0.0 0.0 +589071 0.0 2.0 0.0 1.0 3.0 1.0 3.0 1.0 +531539 0.0 0.0 0.0 0.0 0.0 2.0 0.0 0.0 +183480 0.0 0.0 0.0 2.0 0.0 2.0 1.0 0.0 +583117 1.0 1.0 1.0 0.0 1.0 0.0 1.0 0.0 +589277 0.0 4.0 0.0 2.0 0.0 4.0 1.0 3.0 +580629 2.0 0.0 1.0 0.0 2.0 0.0 0.0 0.0 +535375 3.0 0.0 3.0 2.0 0.0 0.0 0.0 1.0 +363400 0.0 0.0 0.0 0.0 0.0 0.0 0.0 2.0
Binary file test-data/pick_open_reference_otus_otu_table_with_taxo_without_pynast_failure.biom has changed
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/pick_open_reference_otus_representative_sequences.fasta Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,20 @@ +>183480 L1S208_7 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG +>363400 L1S57_77 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAG +>531539 L1S208_60 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGG +>535375 L1S8_4 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG +>580629 L1S105_71 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTT +>583117 L1S76_13 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG +>589071 L1S140_45 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG +>589277 L1S208_15 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>708680 L1S76_81 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGG +>851865 L1S8_28 +AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCG
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/pick_open_reference_otus_representative_set_tree.txt Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,1 @@ +((589071:0.01564,(580629:0.01561,589277:0.00014)0.960:0.04297)0.241:0.02059,583117:0.00014,((183480:0.16413,(851865:0.20153,(363400:0.07236,(531539:0.00867,708680:0.00659)0.893:0.08113)0.886:0.09917)0.903:0.10496)0.964:0.14514,535375:0.03431)0.597:0.00813);
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_fastq_libraries_demultiplexed_sequences.fastq Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,344 @@ +@L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGATGTGAAAGACATGGGCTCAACCTGTGGACTGCATTGGAAACTGTCATA ++ +ACCCGGGG@G?GGGGGGGGDGGGGGGGGGGGGGGGGDGGBGGGBGGGGGGGDGEGGDDG@GGGGEEEGGGGGG388GD>DDGGEE@DDDDB>FDGD>@-:74=;A*74====?BBD@ +@L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTG ++ +GGGGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHFHBHHECCHBDHFEHAE@@BDB=B@@A2D(5553DAAAAE@EEA>;)9;==A>7B@=@78;:82?C<><9?<>>>98=@ +@L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTA ++ +IIIGIIIIGIIIIIGIIHIDIHIHHF@IIFIIIIIGIIIHIHGHBIIIGHHGIFEEEFHHHDEEDG>DBEFBDB@BB=EEBBBEGEDDBA>D@@@@BCBB;BD@@>:@@>B@?=1@@@3<9767=6>;;? +@L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAG ++ +IIIIIGIIIIIIIGIIIIIIIIIIIFIIGEGGDIIIIIFFHIIGDGEGD>GDGIIEIGIIFH>GBDDGFGGEGBB2DBDDGBDEBEBB8?<B>B>A@D=38=AC>?>@@?DD@>=?=478;+;BE8>B +@L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG ++ +IIIIIHIIIIIIHEEIIEIIIIIIGGHIHEIIHDFCIGHHHCEDBIEIEEHBBEBBEIHGGGGGDHGGEEGDG?<2A5DBBBBEBEBBB@BEDEBBBE;@@@B@@8:@B@@@@AE@@A0@>=<9:;9=7>:;==<? +@L1S257_5 HWI-EAS440_0386:1:25:2639:1894#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG ++ +HHHGGEGEGGGGGHHHHHHHGHHGGCGGHFHHHHHHHHEDBGGHFHHHHFHHHHHFHHHHHEBFCEEDFFFDE=;@C=CCCAABBD@@:=;?=BB@>DBD=@DBBB=D6'=;;A=?=99=9.9.2,/2===79@@6@ +@L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGT ++ +IIIIIIIIGIIIDGIIIFIIHIIIIIIIIIIIIIIEIFGFGGGHIIGIIIHBHIHGIGIHI@EG@EGFFIFEDFBC<FEC@B+@BBBB82==?BB@<BB@BB)AA@8=@?@@5?<>=;;2?-?=A? +@L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIBIIIGIIIIIIIIIIFIIIIIIIIIIIHGIIIIIIIEDGFGEDDGDBG:@@ECGEFEBHBHFHBB@@GGBBBDBECBBGECBECBFEBDEBEBB@@2B4?<=?;A??A@A+>? +@L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCGACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAG ++ +IIIIIIIIIIIIHGGIIHIIIIIIIHIIIHIIIIIGIHHIIIIHDIIIHHHDIIIHIIHDHGEHEHGDGHEDGDHEEFEHG,EDCDCF@EBBECB@@B6>@A@ECBBCBBEEA>BBB@@DBAB=@8@@ +@L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTT ++ +GGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHGHHHHGBHHHHHHHHHFHHHHEHHHHEFEHFGGFGAA>C=GGDBDEDEEBCBBEEBB>BD@BB@DBBBB=C>BCCB>EBB8? +@L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAG ++ +HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGBGGGEGGGG@GGDGHHFGHHHHHHGGFEGBGGG3CE>EAABGDBFFEDFE>E>DCB@EFFBC>EDBDD+CAA>>9?=99DCC?ACABEBBEB<BBD@B@@@B +@L1S76_11 HWI-EAS440_0386:1:25:6540:2228#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTA ++ +IIIIIIIIHIIIIIIIIIIIIIIHIIIIIIIIIIIHIIGIIGIIIIHIIIIDGIBFHDHBEHHIEDHGHHIFFGGH@>EECEBGDECDEEEEEED8B>@@@??EEB*B71;2>7@?===@ +@L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTT ++ +IIIIIIIIHIIIHIIIIIIIIIIIIBIIIFIIIIIHIIHHHIIFFIIIFEIFIIIHIIIIIHGIEECHEFIDIBC>GBFFFEEHBEGEBBEFDBCBBF8A??ABBD=B@@DBB??A;;>? +@L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG ++ +IIIIIIIHIIIIIIIIIHIIIIIGIFIIIIIIIIIHIIIHHIHHFIIIGIIIEGIFHIIGHIIIGHGIGIGHIEDBFAEHHFEGGDGE<CBFCGDEGGGGEGHEECE>CBC@BDB@ +@L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAG ++ +HHHHHHHHHHHHHHHHHHHHHHHHHDHHHHHHHGHHHHHHHHHGFGGGFGGEGGGGGGHHHHHGHHHHHHHHHEEEGCHHHGGGHHHBHGH>EEDBDEHHHDFBFG><??CAC8DDABDEEBEDB@BEB@B>B +@L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ++ +IIIIIIIIIIIIGIIIIIIIIIIHIEIIIHIIIGIIIIIIHIHFIIIIIDIHHIGGIIIIHBHIFIFIIIHHIEE@GBGDDDD@EHEEEECBEEEDCFBCBBFEEI>@B@DBDBEEB9???,?<A>@A=A=<=;*;? +@L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAG ++ +IIIIIIIIIIIIGIIIIIIDIIIIIHIIIHIIIIIHIIHHIIIIHIIIHHFHGIHHIGGIEHDICDEIEIIEIBBBD@FBEEBEEEFDDBEFEBBBBEBBBBD?BB88<:<9?B@B@@@A?3@@@9?@ +@L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAA ++ +HHHHHHHHDAGGGHHHHHHHHHHHHHHHHHHHHHGHHHHHHH@HHHHGHHFGHHHHHHHFHHHHGHHHDHHGHGGH<GE@DEBEFEFHGGGBG@BBEDBDB@DEBFEEGEE>GBC>DECEDB>6>>>A6>>;4>=6:=9=B:@ +@L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGG ++ +GGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEHHHHHHHFHHHEHEHHHHHHHBHHEHGHHHFGGGGBEEHEGCBGDCGEGGEGGEGECCEC@EE>B@A==@7,8=>;1=9A>>>;A +@L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ++ +IIIIIIIIIIGIHHIIIIIGIIIIIFIIIHIIIIIHIIIHIIGIGIIIHIIFIIIGIIIDHGDIFIFEHIFBHBDFFFEIE8BDEDDEEFDDHECC@EABCEEBAD3D@BCCCEF>CBBBC8C?B@=@@<<>BB=@@B +@L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGG ++ +GGGHHHHHGEGGGHHHHHHHHHHHHHHHHHGHHHHHBHHHHHHGCGBGEEGEEGGDBBADBGDFDEEGCGFEFFCDBBDBECCEFHEEEE:HB;A<::=?>=;EEEAE@>AC>,:.52?;:5255:,7@3:4:@ +@L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG ++ +IIIGIIIIIIIIGIIIIIIGIGIIHIIIFIHIIIIHHIIIIIEIEIHIDDGG@GGBGGGHIIIIGIFIGIIHHEEEGBHEHHFFGHGGGGEGDEBEDEACDDCABBDDB@@@BEBEBCCBDBBBBDEE?B@>B +@L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ++ +GGGHHHHHBHHHHHHHHHHHHHHHHHHHHHGHHHHHGHHHHHBGHHHHHGHHBGGEGEDGBGGHHH>HDBGDGHHGHED>GB@GEGEGHFBHED@BDDDHFD@C>FCEFEHBB>@?:>??8826=8/:;<<:?>)>A +@L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG ++ +IIIIIIIIIIIIBHHIIHIHIIIIICIIIFIIIIIHIIIHGHHFEIHIEFIIBIHFHIIHIIGHEHGHGHHGHDBBICHEFHEGGDGEECGHEGDE>GGEDEGDDDBEEBEE@BBEBBDGDC=ABBBB?==-:==? +@L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCG ++ +HIIIIIIIIIIIIIIIIIIIIIIIIIDIII3IIGGGGGIIIII@HIIGIIHGIIIIIHIIHIIIHIIDEGGEGGHHIHIHIIGDFIBF<DBDDBIDHHIIGGGFIFEHAACEAEIDI9IIFCFFEEEF<BB<D==???<@ +@L1S76_25 HWI-EAS440_0386:1:25:8159:2639#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTA ++ +IIIIIIIIIIIIIIIIIHIGIIIIIGIIIHIIIIIGIHHHHHHIHIHIDEIGCIFHHIHEIHEHEHEIEIHEIBDBFBFCDDDGEGEEDDDGCBDBBDABEBEDGDEAEBCBCBBEBB@EB7BB@BBB6? +@L1S140_26 HWI-EAS440_0386:1:25:11175:2690#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAGGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGC ++ +CCCGBGGGEGGGGGGGEGGGGGGGGDGGGGGEDGGFGGGGGGGGFDDGFGGEGGGBEGEGDGGGFG>GFGGFGFEEGEFEF@EEEEFEBEDFDCD@BBEDE>EDCFBEDBEF>;A>:>AA8>>;AA +@L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG ++ +IIIIIIIIIIIIFIHIIIIGIIIIIFIIIHIIIIIIIIIIDIHIIIHIGIIHIIIIIIIIFHHIGHGIHIGEDBBBGBGHFHGCGHGEBEEIEEBEECEEHCHBEFBBB@BBBC>E@B?@@?@=@@BB;>@?>@?>A +@L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCG ++ +DGGEBEEE?B,B?GGGGGGGGGGGGGGGGG>GGDGGGGGGGGGGGGGGGGGGGGGA>>G@>G<E?BEE@GGDGGDGBDGEB>DDGDGFFGD8FBB??>GDGDBA@AD;AAAE5?B;;4ADDBBB@B@D1.?8??-8::;? +@L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA ++ +IIIIIIIIIIIIHHHIIIIIIIIHIBIIIFIIIIIHIIIIIIEFIIFIEHFHHIIFIIIIHIHIEIHIHIIFICECIBHHIFEHDEGECBBHEDCBDFCEEBHBBEC>B=BBBCEBDA@BB>B>BB@CB=?@ +@L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG ++ +IIIIIIIIHIIIDIIIIIIIIIIIIFIIIHIIIIIHIIIIHIIIIIIIIIIIIIEIIIIIIIGIHHEIHIHHICEHIHHII@EHEHFEGDGHGEFEEFGGBEHBEE<F@@BDDDCBDBDDBBB>BBBEBEB@@>@=@B +@L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTA ++ +IIIIHIHIEGGGEIIIIIIIIIIIIGIIIHIIIEGBGDDDDGGGIIIBEHIIFHHIIFIIGDBC@>EC3@?>@GEGG@@DBD@E8CC=BBDBEAC,38==:;=@?,3=9-<;<?A?? +@L1S140_32 HWI-EAS440_0386:1:25:14127:2819#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTT ++ +GGGGGGGGHHHHHHHHHHHHHHHGHHHHHHHHHGHHHHHHHHHGEHHHHHHHHHHGHHHHHHGEBEEGEGGFGCCBG@EEFDEEEGDEEBBHEDEBEEGGBEGBAEBD@AHDC?CE@?BDB:>68<===B +@L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTGGTGAAAGATCGAAGCTTAACTTCGGTAAGCCATGGAAACCGTAGGGCT ++ +HHHHHHHHEGGGGHHHHHHHHHHHHFHHHHHHHDBGGFHHHHBGGDDDHHHHGGHHBFGFCGGFBEGGDGD>D>9ACACEEEBEEBBCDB@EF>DBB@C3*=@AA@=@BAEEBCCB@C +@L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAG ++ +IIIIIIIIFIIIIIIIIIIIHIIIHHIIIHIIHIIHIIIFHIIFHIIIHFHIIDHHIHIIIIHIEHDIGIHGFDDCFCB@DEBFEFCFC>EDDBBBBBAAA?A@BB@E@?>-@A@B??@DB-A +@L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCGACCGTAAAATTGCAGTTGATACTGGCTG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIIIHIIGHIIIFHHIHIGIIIIHIIIIGHHIHIGHIECCGCFFIFFHDFGB=@@DBBBBBBDB?@EBBB=B>=B@BBBB +@L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAG ++ +HIIIIIIIIIIIIDIIIHIIIIIIIBIIIGIIIIIGIGIHIFIHHIIIGHIIIFHHIGDFIEEIEFIDFHHGEGBHCFHGEGDGCDEBCCBBEEC>B@B>B8@BBB:B;???9@B>C@>;? +@L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTC ++ +IIIIIIIIIHIIIHIIIIIIIIIIIIIIIIIIIIIIIHIHHHIIHIHIEIIFEHHIIIIIIIHIGIHIBDHEHGHHHGHHEGDBCDF>DDCBFE@?BB@B@78BB;@=@@?@=;A=????@:>=@=B +@L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATAACTTGAGTGCAG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIGIIIHIIIIIHIIHHHIIIHIIIEEIFIIIFHIIIHHEIGGGIGIIGIGGDIEGGHGHDEEABCCEDBC@BBEBBDBFEEGABB@BCC<<?'+=><<;:A0?A +@L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTG ++ +IIIIGIIIIIIIIGGIIIIIIIIIIIIIIIIIIDIIIIIIIIHIHHHIIIHFIIHHIGIIIIHGEIDGFIIHIHFEIEHGIGHGGEG@EEBGEEBDEEEDGEHHDGHHGDDCGBDBB?DBD@B??A8A?@D<B7-? +@L1S76_40 HWI-EAS440_0386:1:25:6132:2959#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCAGGGCTCAACCCCGGGACTGCTTTTGAAACTGCAGGGCTAGAGTGCAGGAGGGGCAAGTGG ++ +A>BGGGGGGGDGGGGGGGG@GGGGGGGGGGGGGGGGGGGGGGGDGGDGCFF>?GGGGGGEGGGGGG8GGGGDGGBG<GEG<GGEDEGGGGEEG1=??BEFD>CE>CECDEBEC?>,+?A=??==B@BBA<>>/=9?8?>>? +@L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGG ++ +IIIIIIIIIIIIIFIIIIIGIIIIIBIIIFIIIGIFIIIHHHHIHIIIFHIGEIIHHIHHIH>IFIEIHHGHIBC>GCFEIEEHGEGDEEDGDBCBBBDDEEH@<BBBEBCBEEFEEBEEE@A:?@BB<58'2<<<69;?A +@L1S57_42 HWI-EAS440_0386:1:25:1831:2983#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGC ++ +EFFGGGGGGBDGGHHHHHHHHHHHHGHHHHGHHHHHHHHHHHHHHHGHBGEGGEG@GGGGEFFGECGGCCEACDDD8G;@A@@@BDDBFEHEDB>2@AEEB>BAA>>9>=AA77>>@? +@L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGCTGAAACTGGCAGTCTTGAGTACAG ++ +BEEGGGGG3EBEDEEEEEGG3<GEED<EGG@EDGGB<GEEEEAB.4BB5>@>,EE<EEGGGGDGFGGG>GGADB??<16/459DFDGFFFDB15*9:/AAAAA(??;;FCCBDDD@>BBDBBB85>;A +@L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGGTGCAAGCGATAATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGGGTGAAATCCCACAGCCCAACTGTGGAACTGCCTTTGATACTGCCAGGCTTGAGTACCG ++ +BBBGGGGGDDGGGACA@,;<58;GDGGGGGGFGGBGGEGGGGGGGGEGEGEGBGEGGGEG8EEFEFEGFBFADCCEBDBFBEEEEAEBBBDD<>A8>>AEEBE@DBB8DB@B0>9)>>@=8@>:3:8@ +@L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG ++ +<@BGGGGGGGGGGGGGGGGGGGGGGDGGGGGEEGGGGGGGGGGGGGGGGGEGGGGGGBEGGGGGEGGGGGGGGBG2GBGDGGDEGDGFEGFGEGFFDEEGDEGEFDEGDDBDFC8FCBDDCCDDBFBC5AA>A>A<AA +@L1S208_46 HWI-EAS440_0386:1:25:5972:3048#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGA ++ +IIIIIHIIIIIIIIIIIIIIGIIIIIHIIII@IIIDIIIIIIIEHIHIHIIHIIIIIFHIIHGIGHEIIIFHIGHGDHHHCGECCDEGHDEDGEBBBEDGDBGCCC?A@=B@@8B@B +@L1S140_47 HWI-EAS440_0386:1:25:9861:3050#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCT ++ +GGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHHHHEHCD@GDHBHHHHEGGEEACE@ECECCBDB<D=D@@A>8AAAA5@89? +@L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG ++ +IIIIIIIIGIIIHHIIIIIIIIIIIEIIIEIIIIIHIIHHHHHHHHIIGHEHIIIHIHFHICGHEFEIEIHEH@=BD@DEFECB:BBBCBCFCBBC=CCC@8E@CBBCBBBBBD8D@;AA@??BDDEGDBAAD=>@@@ +@L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTG ++ +HHHHHHHHDGGGGHHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHHFEHHHHEHHHHHHHHHHHDHHHHGGHGHGDHHHFGGGHHHHHHFHGHGHGCEE2EB<AD@EEEBBEGAGECEBB81?;?? +@L1S105_50 HWI-EAS440_0386:1:25:9110:3081#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG ++ +IIIIIIIIIIIIIGIIIIIIIIHIGHIIIHIIIIIHIIGHIIIIHIIIHIIGIIIHIHIIIHHIGIHIHIIGIDCBIEFHHFHDGHGEDBEGCEDCDECBBBE@DD@BA>A?CB@B@@BDBBB=B@=BBBBBBD7BBB +@L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGG ++ +GGGGGGGGDGGGGHHHHHHFHHHHHHHHHHHHHHHHHHHHHHHHGHEHHHHHHHHEHFEFBGGHGGHHBEGEGFHBEHHBHGHHDHHHGHGBHC8>8?ECDEEEEC<BCEEB@91;==BB:B@BB>B@/:::;5==>>A +@L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG ++ +IIIIIIIIIIIIIIIIIIIHIIIIHHIIIIIIIIIHIIIIFGHHHIGIGHHIIIIIIHIIHHIIGFFIHHHGICHGHGHGGHEDHHGDFGGGGGGDB@BCCBDABE@E@BBBBEEBF@?A>?==B@/=?@?>;@BB@ +@L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG ++ +IIIIIIIIIIIIIGHIIIIDIIIIIGIIIFIIIGIIIIIIIICGGIIFBDGG@IFFIFHGHIHDBECEHGHGFFD8FCGCGGGGFGEBDEEEBEEGEGBB>BB@BBCCA=;??=<?;4>A@=?:>@??:@@(@ +@L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGC ++ +IIIIIIIIIIIIIIIIIIIDIIIIIHGIIIIIIIIGIIIFIHIIHIIIGIHFIIHIIHIGHGGHEH<HGHGEFBC>EBEEEFCGEGDBEECEBBCC<@BDBBEDBDBD@BCBCDDDADB@==@?@B=@@B3B=@@ +@L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGG ++ +HHHHHHHHHHDHFHHHHHHHHHHHHGHHHGHHHHHHHHHHHHHHHHHHGGGG>HHHHHEDGDDGFGGGDGGBGEEGBGG8G@DEGGEFEFE<GGEEFEGGHG3DGBBBDDGD@GFDEEDBDBB>BBBDBBD@>?:;??=?B +@L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAG ++ +HHHGGGGGGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHDHHHHHHHHGG1D<?A;=;GFGBGG<GDGDDGGBAADG@HHBHFGHEGE<?=88DDDAD@@BB2:;?@?B>>D;A9A8C +@L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ++ +HHHH@HHHHHHHHHHHHHHGHHHHHHHHHHHHHHHHHHHHHHHHGHHHGHHHHHHHHHHHHGEHHHFHEGEDGDDDGGFFFDDABEEEDDCFGEEDBCAEBEEEEE@EBBEBBEEEE8B@@:<8==<=BAA>A@:@8@ +@L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGG ++ +IIIIIIIIIIIIIIHIIIIIIIIIIHIIIIIIIIIIIHIHFIIHIIHIHHHIIIHHIHHIIIHIGIHIFHIFHEDBIDFFEHHGGHHEFEBIEFFCEED>EDEBEEDBEEHDBEFEBDGEEDDBFEDEBCBBB;CBBB +@L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAG ++ +IIIIIIIIIIIIIHIIIIIIIIIIIHIIIFIIIHIHIIHHIHHIIIIIHFIIGIFDAGIIHFHHEH@IHIIGIHGGIEHGHEHGHFHGEEEIHHEEE@EEEEIEFHBEDFGDFGGEEDEEBB18A@@@B@88@ +@L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIHIGIIGIIIIGIIIGIIIHIIIIIHHHIIIHIIIHIHHI<HFIFHEIHEHGGHCBHBG<DDDA?@AD@8=BAA?<6?AA??1<<9;2546<?+:@ +@L1S76_61 HWI-EAS440_0386:1:25:3312:3259#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGC ++ +IIIIIII:DGGGGFGIIIIHIIIIHFIIIDIIIIIHIIIHDIIICIIIEHIHIIIHIIIHIIHFEE>IIHHGIGE>FEEEFHEEGGGGCHEHGGEGDEEGG@GDDEDGEBEGDBE<EBEBBBC@@@ +@L1S57_62 HWI-EAS440_0386:1:25:3550:3291#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG ++ +IIIIIIIIIHIIDIIIIIIEIIIIHFHIIIHIIIIHFIIIHIIIIIIIEHGIDIIDIGIFFBIIHEDIEEEFGEHEIBFCFDEGHGGGHGHGE>EEDEBEBBDDCCDDC>DCC@C?CBCE@BBBB>9@<>?;@+;<? +@L1S257_63 HWI-EAS440_0386:1:25:5478:3298#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG ++ +IIIIIIIIIIIIIIIIIIIIIIIIHHIIIHIIIIIHIIIIIHHHHIIIHHIIGIHIIIHHIIIHCIHIFHIFFEE@HDHGHFGGGGGEGEEHEGHECGEEGGHBBDDBB8B@B@@A +@L1S281_64 HWI-EAS440_0386:1:25:15346:3296#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCTGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGT ++ +IIIIIIIIIIIIHIIIIIIIII2GGDGGIHIIIIHIIIIHIHIIEIIIIFIIIIIHIIIIIIGIFFDIIIIHIEEDIEIEFHHBEICEBDCEBECDEEEHEBFBCEBDB=BBD?AA? +@L1S76_65 HWI-EAS440_0386:1:25:3468:3322#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGCTGTGCAAGACAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGTGACTGCACGGCTAGAGTTTGTCAGAGGAGG ++ +IIIIHIIIGGGGGIHIIIIHBIIIHIIIIIIIIIGIIHHHHIIIGIIDIHIIIIHHIFEIEEEFDCFFGEGDHGGEGGGGHGEEDGGEEDDGF8EBBCDEEDBBE@@@BCBCBB@EBBBB@==4@??3===99@1B@ +@L1S281_66 HWI-EAS440_0386:1:25:4199:3321#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCATCACAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTTGAAACTGTGGAGCTGGAGTGCAGGAGAGGCAAGTGG ++ +IIIIIIIIIGIIIGIIIIIIIIIIHDIIIIIIIIIHIIGGEGGIHIIIIIHIEIIIEIIIHGIIHIIGIGIEIHHIHHHGEGEC<EHCGGHEFE<EB@BBE>CCEB>B;/=?=BCBCB>BB>@@B<@?<85*928578=@B +@L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ++ +GGGHGHHHHHHHGHHHHHHFHHHHHFHHHHHHHHHHHHHHHHHHEHHHEEGDGHHHHHHHHHHHGHHHHHHHHDGGHGHHHHFEHGGBHBEHEGHGHGGHGDHAEHEHBBDBBBBC>BEEEBBAEAAEEBEAB><@B +@L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG ++ +HHHHHHHHEHHHHHHHHHHGHHHHHHHHHDHHHHHHHHHHHHHHHHHHHHDHHHHEFHHHHH@HHHHHEHHEGEEEHDHEFHEGHE@EHGGHGC@B8DEEECEBEED8;?=??DBCDBCCCBDBB<BBBCE8E3=3=? +@L1S140_69 HWI-EAS440_0386:1:25:4994:3354#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ++ +GGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGFHHHHGGHFHHHHHHHHHHHHGHGHEHHEHD>CECGGHH@GGCFGGEGHGB@BBBEEEEH@BBCCB@B>CE<C@BBEBBADEAA>=A@@=:==? +@L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGG ++ +EEEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDGGGGGGGGGGFGFGGGGDGFGGF>EGEGEFED8DGDEEEBGDGBDFDBDBDEE<BED@@A@8B;=9>8>+>;;49;? +@L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTT ++ +IIIIIHIDIIIIIDIIIIIIIIIIGDIIIEIIIIIIIIFFFIIIHIIIHEIIIIGFIIIGIIHIEHEIFDIFIGGGHEIEDHCGHGHHDEEIEEEEEEGGG>GDEDBB>ADAD@A;>4<=<967:<<=:? +@L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGGAC ++ +HHHHHHHHHHHHHHDHHHHHHHHHHHGHHHHHHHHEHHGGBGGGHHHHHHHHHHGHHHHHHGEGFFGFFGFDFEFHFDHEEHBC2BEC8=??ABBB@D@@>BBBB@5@77==1CC@BB +@L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAG ++ +GGGIIIIIIIIIIIHIIIIGIIIIIDIIIGIIIIIIIHIIIGIIGIIHEGGGCIIFIIIHIIIIHGHIHHIEHHEDDHFBIDFFHHFEGGGHG>EEBEBCEBC@BBBB=69=,:186:EBEBC;;@:@ +@L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA ++ +IIIIIIIIIIIIGIIIIHIIIIIIHHIIIIIIIIIHIIHHIIIIHIIIHGHHIIIHHIHIIIHIEEEIHIIGIFCDIBHHI@HHGEDD>B@DBEBBBEEEBEFCEGEEB<DBBEEBEB@BB<C=B7@B@CB@ +@L1S105_75 HWI-EAS440_0386:1:25:9796:3444#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAAGTGAAAATCCGGGGCTCAACCCCGGAACTGCTTTTGAAACTGTGAGGCTAGAGTGCAGGAGGGGTGAGTGGAATTCCTAGTG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIDIIIIIIIHIHIIGIIGIIHIIIIIIHHIBIIIIIIIGIEIHGIIIEHII@IGIHFDHIGDIHHFEEIGBB<DDFFFFEFDEBBDGGBFCEECDDD@D@FBBDD;BBAA@D9D::?3=<65855;:< +@L1S8_76 HWI-EAS440_0386:1:25:2235:3462#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAGTCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCA ++ +HHHGEGGGFGBGGHHHHHHFCHHHHHHHHHHHHHHDHEFFFG@EEEF<HFHHHHDHF8DHBHHHAGHGFFDADDDFCBEEDDACEBEBEB7>E>>)>@EDCC@B<>:5:6:>68>0A;9>;:@?<>??@B +@L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAG ++ +GGGHHHHHHHGHHHHHHHHGHHHHHGHHHHHHHHHHHHHHHHGHHHHHHHHHHHGBHHHHHBHHHHHHHFHGHBHHFGEGEEBEHHHDDDB@GB=?=?EEE>EBD3=@AABDDD>BBCA<A=A +@L1S281_78 HWI-EAS440_0386:1:25:13919:3507#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGG ++ +IIIIIHIIFIIIGGHIIHIDIHIIGBIIIEIIIHHEIIHEGHHHEHGIDBEGBIFEFFEEHEBHEFCHGGHEHE@BHBFDFEEGEDDEBBBHE@DBBDBEBBEDEEDBE>AECDCDB@BB@:C@BB@B=0=0>?=>@ +@L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAAGTGAAATGCGGGGGCTCAACCCCTGAACTGCTTTGGAAACTGTCATGCTAGAGTGCAGGAGGGGTGAGCGGAATTCCTAGTG ++ +IIIIIIIHIIIIHIIIIIIIIIIIIIIIIIIIIHIIIIIIHIFIIIIIHHIFIIIHGHHIHIIIHHIIFFICIB8BDGD@DBACFEDGCEEBFFFCBBBEBEBAA;8?>?AAABBCC@BB>>C@DB@B@7@@@83;8@89<7+=8=(=5?9? +@L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTAATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTG ++ +<@@GGGGGGGBGGGGGGGGGGGGGGGGGGGGDEGGGGGGGGGGGBGGGGGGDGGGB<BGGGGGGGGGG><GGGDGDGGGEGDGGGGGEGFE<DGDDDDGGGBGDDGD:BBDDD??:<?GGDBB;B +@L1S76_81 HWI-EAS440_0386:1:25:11581:3662#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGG ++ +IIIGIIIIGHIIGGIIIIIHIIIIHIHIIIHIIIIEIEIFFGGGIFIHIIHFIHIHDFIIIGEHEHHIHIGGFEGG<HEGBD>@>ADAEEE@D?A=<=@B@@@B@B>BBBBCA@@)>@<4>1>@@>;>A4:@A +@L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAGTTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG ++ +IIIIIIIIIIIIIIIIIIIHIIIIIDIIIIIIIIIHIIHIIIIIHIIIHDIFIIIIIIIIIIHIGIGIHHIEIFFEIEIHIFIGGHGHDHHHGHGB<EEDHEFGGHBGEDHHHBCBEBEED@B8@@@A@B@@@8?3? +@L1S208_83 HWI-EAS440_0386:1:25:8197:3691#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGAGTGTCG ++ +IIIIIIIIIIIIHIIIIIIIIIIIHGIIIHIIIIIIIIIHHIIIIIGFIIIIIIIIIIIIIHGIFFGIFIIFIGHBIHGIHIGHGGEDDBDBFD>B@@BBB=BBDB?@B:BBBEEECB??A4@4?==? +@L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAG ++ +=B@GDGGGEGEGGGGGGGGEGGGGGDGGGGEEEGGGBGGGGGGGDGGGGGGG@EEBBE???DBGGGEGBGGDGGGGBC?0?<BFGF>BDGDDGCBDDDDDDDDEGGFDDDAG+(2:39DBAAA +@L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCGGTGAAATTTTGGTGCTTAACACCAAACGTGCCGTTGATACTGCTGGGCTAGAGAGTAGTTGCGG ++ +IIIIIIIIHIIIIIIIIIIGIIIIFFIIIFIIIIIHIIIHEIIIHIIIHHIFIIIIHIIIHHHIGHHFHIHEIDBBFGHFHFEHEIIEBDBEEDCDEF=DBDDCBD=D@BDEBDFCC>B@B4B=@=>@=BB>8?
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_fastq_libraries_histograms.tabular Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,8 @@ +Length Count +116.0 20 +126.0 31 +136.0 33 +146.0 2 +156.0 0 +-- +
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_fastq_libraries_log.txt Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,53 @@ +Input file paths +Mapping filepath: /home/cidam/Berenice/asaim/framework/lib/galaxy-master/database/files/000/dataset_15.dat (md5: 4f17f8d2fbfd80c72d7958f39c5c5286) +Sequence read filepath: /home/cidam/Berenice/asaim/framework/lib/galaxy-master/database/files/000/dataset_12.dat (md5: 9d4f2526cd83c61358d737e8ead741bd) +Barcode read filepath: /home/cidam/Berenice/asaim/framework/lib/galaxy-master/database/files/000/dataset_11.dat (md5: 3856d8f889198dc37ffd61b231c03257) + +Quality filter results +Total number of input sequences: 250 +Barcode not in mapping file: 134 +Read too short after quality truncation: 21 +Count of N characters exceeds limit: 6 +Illumina quality digit = 0: 0 +Barcode errors exceed max: 3 + +Result summary (after quality filtering) +Median sequence length: 132.50 +L1S76 14 +L1S281 12 +L1S8 11 +L1S208 11 +L1S140 11 +L1S105 10 +L1S257 9 +L1S57 8 +L6S93 0 +L6S68 0 +L6S20 0 +L5S240 0 +L5S222 0 +L5S203 0 +L5S174 0 +L5S155 0 +L5S104 0 +L4S63 0 +L4S137 0 +L4S112 0 +L3S378 0 +L3S360 0 +L3S341 0 +L3S313 0 +L3S294 0 +L3S242 0 +L2S382 0 +L2S357 0 +L2S309 0 +L2S240 0 +L2S222 0 +L2S204 0 +L2S175 0 +L2S155 0 + +Total number seqs written 86 +--- +
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_fastq_libraries_sequence_qualities.qual Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,584 @@ +>L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[32 34 34 34 38 38 38 38 31 38 30 38 38 38 38 38 38 38 38 35 38 38 38 38 38 + 38 38 38 38 38 38 38 38 38 38 38 35 38 38 33 38 38 38 33 38 38 38 38 38 38 + 38 35 38 36 38 38 35 35 38 31 38 38 38 38 36 36 36 38 38 38 38 38 38 18 23 + 23 38 35 29 35 35 38 38 36 36 31 35 35 35 35 33 29 37 35 38 35 29 31 12 25 + 22 19 28 26 32 9 22 19 28 28 28 28 30 33 33 35 31] +>L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 37 + 39 33 39 39 36 34 34 39 33 35 39 37 36 39 32 36 31 31 33 35 33 28 33 31 31 + 32 17 35 7 20 20 20 18 35 32 32 32 32 36 31 36 36 32 29 26 8 24 26 28 28 + 32 29 22 33 31 28 31 22 23 26 25 23 17 30 34 27 29 27 24 30 27 29 29 29 24 + 23 28 31] +>L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 38 40 40 40 40 38 40 40 40 40 40 38 40 40 39 40 35 40 39 40 39 39 + 37 31 40 40 37 40 40 40 40 40 38 40 40 40 39 40 39 38 39 33 40 40 40 38 39 + 39 38 40 37 36 36 36 37 39 39 39 35 36 36 35 38 29 35 33 36 37 33 35 33 31 + 33 33 28 36 36 33 33 33 36 38 36 35 35 33 32 29 35 31 31 31 31 33 34 33 33 + 26 33 35 31 31 29 25 31 31 29 33 31 30 28 16 31 31 31 18 27 24 22 21 22 28 + 21 29 26 26 30] +>L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 38 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 40 40 40 + 37 40 40 38 36 38 38 35 40 40 40 40 40 37 37 39 40 40 38 35 38 36 38 35 29 + 38 35 38 40 40 36 40 38 40 40 37 39 29 38 33 35 35 38 37 38 38 36 38 33 33 + 17 35 33 35 35 38 33 35 36 33 36 33 33 23 30 27 33 29 33 29 32 31 35 28 18 + 23 28 32 34 29 30 29 31 31 30 35 35 31 29 28 30 28 19 22 23 26 10 26 33 36 + 23 29 33] +>L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 39 40 40 40 40 40 40 39 36 36 40 40 36 40 40 40 40 40 40 38 + 38 39 40 39 36 40 40 39 35 37 34 40 38 39 39 39 34 36 35 33 40 36 40 36 36 + 39 33 33 36 33 33 36 40 39 38 38 38 38 38 35 39 38 38 36 36 38 35 38 30 27 + 17 32 20 35 33 33 33 33 36 33 36 33 33 33 31 33 36 35 36 33 33 33 36 26 31 + 31 31 33 31 31 23 25 31 33 31 31 31 31 32 36 31 31 32 15 31 29 28 27 24 25 + 26 24 28 22 29 25 26 28 28 27 30] +>L1S257_5 HWI-EAS440_0386:1:25:2639:1894#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[39 39 39 38 38 36 38 36 38 38 38 38 38 39 39 39 39 39 39 39 38 39 39 38 38 + 34 38 38 39 37 39 39 39 39 39 39 39 39 36 35 33 38 38 39 37 39 39 39 39 37 + 39 39 39 39 39 37 39 39 39 39 39 36 33 37 34 36 36 35 37 37 37 35 36 28 26 + 31 34 28 34 34 34 32 32 33 33 35 31 31 25 28 26 30 28 33 33 31 29 35 33 35 + 28 31 35 33 33 33 28 35 21 6 28 26 26 32 28 30 28 24 24 28 24 13 24 13 17 + 11 14 17 28 28 28 22 24 31 31 21 31] +>L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 40 40 40 38 40 40 40 35 38 40 40 40 37 40 40 39 40 40 40 40 + 40 40 40 40 40 40 40 40 40 40 36 40 37 38 37 38 38 38 39 40 40 38 40 40 40 + 39 33 39 40 39 38 40 38 40 39 40 31 36 38 31 36 38 37 37 40 37 36 35 37 33 + 34 27 37 36 34 31 33 10 31 33 33 33 33 23 17 28 28 30 33 33 31 27 33 33 31 + 33 33 8 32 32 31 23 28 31 30 31 31 20 30 27 29 28 26 26 17 30 12 30 28 32 + 30] +>L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 33 40 40 40 38 40 40 40 40 40 40 40 40 40 40 37 40 40 40 40 40 40 40 40 40 + 40 40 39 38 40 40 40 40 40 40 40 36 35 38 37 38 36 35 35 38 35 33 38 25 31 + 31 36 34 38 36 37 36 33 39 33 39 37 39 33 33 31 31 38 38 33 33 33 35 33 36 + 34 33 33 38 36 34 33 36 34 33 37 36 33 35 36 33 36 33 33 31 31 17 33 19 30 + 27 28 30 26 32 30 30 32 31 32 10 29 30] +>L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 39 38 38 40 40 39 40 40 40 40 40 40 40 + 39 40 40 40 39 40 40 40 40 40 38 40 39 39 40 40 40 40 39 35 40 40 40 39 39 + 39 35 40 40 40 39 40 40 39 35 39 38 36 39 36 39 38 35 38 39 36 35 38 35 39 + 36 36 37 36 39 38 11 36 35 34 35 34 37 31 36 33 33 36 34 33 31 31 33 21 29 + 31 32 31 36 34 33 33 34 33 33 36 36 32 29 33 33 33 31 31 35 33 32 33 28 31 + 23 31 31] +>L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 38 39 39 39 39 38 39 39 39 39 38 33 39 39 39 39 39 + 39 39 39 39 37 39 39 39 39 36 39 39 39 39 36 37 36 39 37 38 38 37 38 32 32 + 29 34 28 38 38 35 33 35 36 35 36 36 33 34 33 33 36 36 33 33 29 33 35 31 33 + 33 31 35 33 33 33 33 28 34 29 33 34 34 33 29 36 33 33 23 30] +>L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 38 33 38 38 38 36 38 38 38 38 31 38 38 35 38 39 39 + 37 38 39 39 39 39 39 39 38 38 37 36 38 33 38 38 38 18 34 36 29 36 32 32 33 + 38 35 33 37 37 36 35 37 36 29 36 29 35 34 33 31 36 37 37 33 34 29 36 35 33 + 35 35 10 34 32 32 29 29 24 30 28 24 24 35 34 34 30 32 34 32 33 36 33 33 36 + 33 27 33 33 35 31 33 31 31 31 33] +>L1S76_11 HWI-EAS440_0386:1:25:6540:2228#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 + 40 40 40 40 40 40 40 40 40 40 39 40 40 38 40 40 38 40 40 40 40 39 40 40 40 + 40 35 38 40 33 37 39 35 39 33 36 39 39 40 36 35 39 38 39 39 40 37 37 38 38 + 39 31 29 36 36 34 36 33 38 35 36 34 35 36 36 36 36 36 36 35 23 33 29 31 31 + 31 30 30 36 36 33 9 33 22 16 26 17 29 22 31 30 28 28 28 31] +>L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 39 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 + 33 40 40 40 37 40 40 40 40 40 39 40 40 39 39 39 40 40 37 37 40 40 40 37 36 + 40 37 40 40 40 39 40 40 40 40 40 39 38 40 36 36 34 39 36 37 40 35 40 33 34 + 29 38 33 37 37 37 36 36 39 33 36 38 36 33 33 36 37 35 33 34 33 33 37 23 32 + 30 30 32 33 33 35 28 33 31 31 35 33 33 30 30 32 26 26 29 30] +>L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 38 40 + 37 40 40 40 40 40 40 40 40 40 39 40 40 40 39 39 40 39 39 37 40 40 40 38 40 + 40 40 36 38 40 37 39 40 40 38 39 40 40 40 38 39 38 40 38 40 38 39 40 36 35 + 33 37 32 36 39 39 37 36 38 38 35 38 36 27 34 33 37 34 38 35 36 38 38 38 38 + 36 38 39 36 36 34 36 29 34 33 34 31 33 35 33 31] +>L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 35 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 38 37 38 38 38 37 38 + 38 36 38 38 38 38 38 38 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 36 36 + 36 38 34 39 39 39 38 38 38 39 39 39 33 39 38 39 29 36 36 35 33 35 36 39 39 + 39 35 37 33 37 38 29 27 30 30 34 32 34 23 35 35 32 33 35 36 36 33 36 35 33 + 31 33 36 33 31 33 29 33] +>L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 40 40 39 40 + 36 40 40 40 39 40 40 40 38 40 40 40 40 40 40 39 40 39 37 40 40 40 40 40 35 + 40 39 39 40 38 38 40 40 40 40 39 33 39 40 37 40 37 40 40 40 39 39 40 36 36 + 31 38 33 38 35 35 35 35 31 36 39 36 36 36 36 34 33 36 36 36 35 34 37 33 34 + 33 33 37 36 36 40 29 31 33 31 35 33 35 33 36 36 33 24 30 30 30 11 30 27 32 + 29 31 32 28 32 28 27 28 26 9 26 30] +>L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 35 40 40 40 40 40 + 39 40 40 40 39 40 40 40 40 40 39 40 40 39 39 40 40 40 40 39 40 40 40 39 39 + 37 39 38 40 39 39 40 38 38 40 36 39 35 40 34 35 36 40 36 40 40 36 40 33 33 + 33 35 31 37 33 36 36 33 36 36 36 37 35 35 33 36 37 36 33 33 33 33 36 33 33 + 33 33 35 30 33 33 23 23 27 25 27 24 30 33 31 33 31 31 31 32 30 18 31 31 31 + 24 30 31] +>L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[39 39 39 39 39 39 39 39 35 32 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 31 39 39 39 39 38 39 39 + 37 38 39 39 39 39 39 39 39 37 39 39 39 39 38 39 39 39 35 39 39 38 39 38 38 + 39 27 38 36 31 35 36 33 36 37 36 37 39 38 38 38 33 38 31 33 33 36 35 33 35 + 33 31 35 36 33 37 36 36 38 36 36 29 38 33 34 29 35 36 34 36 35 33 29 21 29 + 29 29 32 21 29 29 26 19 29 28 21 25 28 24 28 33 25 31] +>L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 36 39 39 39 39 39 39 39 37 39 39 39 36 39 36 39 39 39 39 39 39 39 33 + 39 39 36 39 38 39 39 39 37 38 38 38 38 33 36 36 39 36 38 34 33 38 35 34 38 + 36 38 38 36 38 38 36 38 36 34 34 36 34 31 36 36 29 33 31 32 28 28 31 22 11 + 23 28 29 26 16 28 24 32 29 29 29 26 32] +>L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 38 40 39 39 40 40 40 40 40 38 40 40 40 40 40 + 37 40 40 40 39 40 40 40 40 40 39 40 40 40 39 40 40 38 40 38 40 40 40 39 40 + 40 37 40 40 40 38 40 40 40 35 39 38 35 40 37 40 37 36 39 40 37 33 39 33 35 + 37 37 37 36 40 36 23 33 35 36 35 35 36 36 37 35 35 39 36 34 34 31 36 32 33 + 34 36 36 33 32 35 18 35 31 33 34 34 34 36 37 29 34 33 33 33 34 23 34 30 33 + 31 28 31 31 27 27 29 33 33 28 31 31 33] +>L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[38 38 38 39 39 39 39 39 38 36 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 38 39 39 39 39 39 33 39 39 39 39 39 39 38 34 38 33 38 36 36 + 38 36 36 38 38 35 33 33 32 35 33 38 35 37 35 36 36 38 34 38 37 36 37 37 34 + 35 33 33 35 33 36 34 34 36 37 39 36 36 36 36 25 39 33 26 32 27 25 25 28 30 + 29 28 26 36 36 36 32 36 31 29 32 34 29 11 25 13 20 17 30 26 25 20 17 20 20 + 25 11 22 31 18 25 19 25 31] +>L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 38 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 38 40 38 40 40 39 + 40 40 40 37 40 39 40 40 40 40 39 39 40 40 40 40 40 36 40 36 40 39 40 35 35 + 38 38 31 38 38 33 38 38 38 39 40 40 40 40 38 40 37 40 38 40 40 39 39 36 36 + 36 38 33 39 36 39 39 37 37 38 39 38 38 38 38 36 38 35 36 33 36 35 36 32 34 + 35 35 34 32 33 33 35 35 33 31 31 31 33 36 33 36 33 34 34 33 35 33 33 33 33 + 35 36 36 30 33 31 29 33] +>L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[38 38 38 39 39 39 39 39 33 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 38 39 39 39 39 39 38 39 39 39 39 39 33 38 39 39 39 39 39 38 + 39 39 33 38 38 36 38 36 35 38 33 38 38 39 39 39 29 39 35 33 38 35 38 39 39 + 38 39 36 35 29 38 33 31 38 36 38 36 38 39 37 33 39 36 35 31 33 35 35 35 39 + 37 35 31 34 29 37 34 36 37 36 39 33 33 29 31 30 25 29 30 30 23 23 17 21 28 + 23 14 25 26 27 27 25 30 29 8 29 32] +>L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 33 39 39 40 40 39 40 39 40 40 40 40 40 + 34 40 40 40 37 40 40 40 40 40 39 40 40 40 39 38 39 39 37 36 40 39 40 36 37 + 40 40 33 40 39 37 39 40 40 39 40 40 38 39 36 39 38 39 38 39 39 38 39 35 33 + 33 40 34 39 36 37 39 36 38 38 35 38 36 36 34 38 39 36 38 35 36 29 38 38 36 + 35 36 38 35 35 35 33 36 36 33 36 36 31 33 33 36 33 33 35 38 35 34 28 32 33 + 33 33 33 30 28 28 12 25 28 28 30] +>L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 40 35 40 40 40 18 40 40 38 38 38 38 38 40 40 40 40 40 31 39 40 40 38 40 40 + 39 38 40 40 40 40 40 39 40 40 39 40 40 40 39 40 40 35 36 38 38 36 38 38 39 + 39 40 39 40 39 40 40 38 35 37 40 33 37 27 35 33 35 35 33 40 35 39 39 40 40 + 38 38 38 37 40 37 36 39 32 32 34 36 32 36 40 35 40 24 40 40 37 34 37 37 36 + 36 36 37 27 33 33 27 35 28 28 30 30 30 27 31] +>L1S76_25 HWI-EAS440_0386:1:25:8159:2639#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 38 40 40 40 40 40 + 38 40 40 40 39 40 40 40 40 40 38 40 39 39 39 39 39 39 40 39 40 39 40 35 36 + 40 38 34 40 37 39 39 40 39 36 40 39 36 39 36 39 36 40 36 40 39 36 40 33 35 + 33 37 33 37 34 35 35 35 38 36 38 36 36 35 35 35 38 34 33 35 33 33 35 32 33 + 36 33 36 35 38 35 36 32 36 33 34 33 34 33 33 36 33 33 31 36 33 22 33 33 31 + 33 33 33 21 30] +>L1S140_26 HWI-EAS440_0386:1:25:11175:2690#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[34 34 34 38 33 38 38 38 36 38 38 38 38 38 38 38 36 38 38 38 38 38 38 38 38 + 35 38 38 38 38 38 36 35 38 38 37 38 38 38 38 38 38 38 38 37 35 35 38 37 38 + 38 36 38 38 38 33 36 38 36 38 35 38 38 38 37 38 29 38 37 38 38 37 38 37 36 + 36 38 36 37 36 37 31 36 36 36 36 37 36 33 36 35 37 35 34 35 31 33 33 36 35 + 36 29 36 35 34 37 33 36 35 33 36 37 29 26 32 29 25 29 32 32 23 29 29 26 32 + 32] +>L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 37 40 39 40 40 40 40 38 40 40 40 40 40 + 37 40 40 40 39 40 40 40 40 40 40 40 40 40 40 35 40 39 40 40 40 39 40 38 40 + 40 39 40 40 40 40 40 40 40 40 37 39 39 40 38 39 38 40 39 40 38 36 35 33 33 + 33 38 33 38 39 37 39 38 34 38 39 38 36 33 36 36 40 36 36 33 36 36 34 36 36 + 39 34 39 33 36 37 33 33 33 31 33 33 33 34 29 36 31 33 30 31 31 30 31 28 31 + 31 33 33 26 29 31 30 29 31 30 29 32] +>L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[35 38 38 36 33 36 36 36 30 33 11 33 30 38 38 38 38 38 38 38 38 38 38 38 38 + 38 38 38 38 38 29 38 38 35 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 38 38 38 38 38 32 29 29 38 31 29 38 27 36 30 33 36 36 31 38 38 35 38 38 35 + 38 33 35 38 36 33 29 35 35 38 35 38 37 37 38 35 23 37 33 33 30 30 29 38 35 + 38 35 33 32 31 32 35 26 32 32 32 36 20 30 33 26 26 19 32 35 35 33 33 33 31 + 33 31 35 16 13 30 23 30 30 12 23 25 25 26 30] +>L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 39 39 39 40 40 40 40 40 40 40 40 39 40 + 33 40 40 40 37 40 40 40 40 40 39 40 40 40 40 40 40 36 37 40 40 37 40 36 39 + 37 39 39 40 40 37 40 40 40 40 39 40 39 40 36 40 39 40 39 40 40 37 40 34 36 + 34 40 33 39 39 40 37 36 39 35 36 38 36 34 33 33 39 36 35 34 33 35 37 34 36 + 36 33 39 33 33 36 34 29 33 28 33 33 33 34 36 33 35 32 31 33 33 29 33 29 33 + 33 31 34 33 28 30 31] +>L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[40 40 40 40 40 40 40 40 39 40 40 40 35 40 40 40 40 40 40 40 40 40 40 40 40 + 37 40 40 40 39 40 40 40 40 40 39 40 40 40 40 39 40 40 40 40 40 40 40 40 40 + 40 40 40 40 36 40 40 40 40 40 40 40 38 40 39 39 36 40 39 40 39 39 40 34 36 + 39 40 39 39 40 40 31 36 39 36 39 37 36 38 35 38 39 38 36 37 36 36 37 38 38 + 33 36 39 33 36 36 27 37 31 31 33 35 35 35 34 33 35 33 35 35 33 33 33 29 33 + 33 33 36 33 36 33 31 31 29 31 28 31 33] +>L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 39 40 39 40 36 38 38 38 36 40 40 40 40 40 40 40 40 40 40 40 40 + 38 40 40 40 39 40 40 40 36 38 33 38 35 35 35 35 38 38 38 40 40 40 33 36 39 + 40 40 37 39 39 40 40 37 40 40 38 35 33 34 31 29 36 34 18 31 30 29 31 38 36 + 38 38 31 31 35 33 35 31 36 23 34 34 28 33 33 35 33 36 32 34 11 18 23 28 28 + 25 26 28 31 30 11 18 28 24 12 27 26 27 30 32 30 30] +>L1S140_32 HWI-EAS440_0386:1:25:14127:2819#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 + 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 38 36 39 39 39 39 39 + 39 39 39 39 39 38 39 39 39 39 39 39 38 36 33 36 36 38 36 38 38 37 38 34 34 + 33 38 31 36 36 37 35 36 36 36 38 35 36 36 33 33 39 36 35 36 33 36 36 38 38 + 33 36 38 33 32 36 33 35 31 32 39 35 34 30 34 36 31 30 33 35 33 25 29 21 23 + 27 28 28 28 33] +>L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[39 39 39 39 39 39 39 39 36 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 37 39 39 39 39 39 39 39 35 33 38 38 37 39 39 39 39 33 38 38 35 35 35 39 39 + 39 39 38 38 39 39 33 37 38 37 34 38 38 37 33 36 38 38 35 38 35 29 35 29 24 + 32 34 32 34 36 36 36 33 36 36 33 33 34 35 33 31 36 37 29 35 33 33 31 34 18 + 9 28 31 32 32 31 28 31 33 32 36 36 33 34 34 33 31 34] +>L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 37 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 39 + 39 40 40 40 39 40 40 39 40 40 39 40 40 40 37 39 40 40 37 39 40 40 40 39 37 + 39 40 40 35 39 39 40 39 40 40 40 40 39 40 36 39 35 40 38 40 39 38 37 35 35 + 34 37 34 33 31 35 36 33 37 36 37 34 37 34 29 36 35 35 33 33 33 33 33 32 32 + 32 30 32 31 33 33 31 36 31 30 29 12 31 32 31 33 30 30 31 35 33 12 32] +>L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 38 39 40 40 40 37 39 + 39 40 39 40 38 40 40 40 40 39 40 40 40 40 38 39 39 40 39 40 38 39 40 36 34 + 34 38 34 37 37 40 37 37 39 35 37 38 33 28 31 31 35 33 33 33 33 33 33 35 33 + 30 31 36 33 33 33 28 33 29 28 33 31 33 33 33 33] +>L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[39 40 40 40 40 40 40 40 40 40 40 40 40 35 40 40 40 39 40 40 40 40 40 40 40 + 33 40 40 40 38 40 40 40 40 40 38 40 38 40 39 40 37 40 39 39 40 40 40 38 39 + 40 40 40 37 39 39 40 38 35 37 40 36 36 40 36 37 40 35 37 39 39 38 36 38 33 + 39 34 37 39 38 36 38 35 38 34 35 36 33 34 34 33 33 36 36 34 29 33 31 33 29 + 33 23 31 33 33 33 25 33 26 30 30 30 24 31 33 29 34 31 29 26 30] +>L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 39 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 + 40 40 40 40 40 40 40 40 40 40 40 40 39 40 39 39 39 40 40 39 40 39 40 36 40 + 40 37 36 39 39 40 40 40 40 40 40 40 39 40 38 40 39 40 33 35 39 36 39 38 39 + 39 39 38 39 39 36 38 35 33 34 35 37 29 35 35 34 33 37 36 31 30 33 33 31 33 + 31 22 23 33 33 26 31 28 31 31 30 31 28 26 32 28 30 30 30 30 31 25 29 28 31 + 28 33] +>L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 38 40 40 40 39 40 40 40 40 40 39 40 40 39 39 39 40 40 40 39 40 40 40 36 36 + 40 37 40 40 40 37 39 40 40 40 39 39 36 40 38 38 38 40 38 40 40 38 40 38 38 + 35 40 36 38 38 39 38 39 35 36 36 32 33 34 34 36 35 33 34 31 33 33 36 33 33 + 35 33 37 36 36 38 32 33 33 31 33 34 34 27 27 30 6 10 28 29 27 27 26 25 32 + 15 30 32] +>L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 38 40 40 40 40 40 40 40 40 38 38 40 40 40 40 40 40 40 40 40 40 + 40 40 40 40 40 40 40 40 35 40 40 40 40 40 40 40 40 39 40 39 39 39 40 40 40 + 39 37 40 40 39 39 40 38 40 40 40 40 39 38 36 40 35 38 37 40 40 39 40 39 37 + 36 40 36 39 38 40 38 39 38 38 36 38 31 36 36 33 38 36 36 33 35 36 36 36 35 + 38 36 39 39 35 38 39 39 38 35 35 34 38 33 35 33 33 30 35 33 35 31 33 30 30 + 32 23 32 30 31 35 27 33 22 12 30] +>L1S76_40 HWI-EAS440_0386:1:25:6132:2959#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[32 29 33 38 38 38 38 38 38 38 35 38 38 38 38 38 38 38 38 31 38 38 38 38 38 + 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 35 38 38 35 38 34 37 + 37 29 30 38 38 38 38 38 38 36 38 38 38 38 38 38 23 38 38 38 38 35 38 38 33 + 38 27 38 36 38 27 38 38 36 35 36 38 38 38 38 36 36 38 16 28 30 30 33 36 37 + 35 29 34 36 29 34 36 34 35 36 33 36 34 30 29 11 10 30 32 28 30 30 28 28 33 + 31 33 33 32 27 29 29 14 28 24 30 23 30 29 29 30] +>L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 37 40 40 40 40 40 38 40 40 40 40 40 + 33 40 40 40 37 40 40 40 38 40 37 40 40 40 39 39 39 39 40 39 40 40 40 37 39 + 40 38 36 40 40 39 39 40 39 39 40 39 29 40 37 40 36 40 39 39 38 39 40 33 34 + 29 38 34 37 36 40 36 36 39 38 36 38 35 36 36 35 38 35 33 34 33 33 33 35 35 + 36 36 39 31 27 33 33 33 36 33 34 33 36 36 37 36 36 33 36 36 36 31 32 25 30 + 31 33 33 27 20 23 6 17 27 27 27 21 24 26 30 32] +>L1S57_42 HWI-EAS440_0386:1:25:1831:2983#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[36 37 37 38 38 38 38 38 38 33 35 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 38 39 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 33 38 + 36 38 38 36 38 31 38 38 38 38 36 37 37 38 36 34 38 38 34 34 36 32 34 35 35 + 35 23 38 26 31 32 31 31 31 33 35 35 33 37 36 39 36 35 33 29 17 31 32 36 36 + 33 29 33 32 32 29 29 24 29 28 32 32 22 22 29 29 31 30] +>L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[33 36 36 38 38 38 38 38 18 36 33 36 35 36 36 36 36 36 38 38 18 27 38 36 36 + 35 27 36 38 38 31 36 35 38 38 33 27 38 36 36 36 36 32 33 13 19 33 33 20 29 + 31 29 11 36 36 27 36 36 38 38 38 38 35 38 37 38 38 38 29 38 38 32 35 33 30 + 30 27 16 21 14 19 20 24 35 37 35 38 37 37 37 35 33 16 20 9 24 25 14 32 32 + 32 32 32 7 30 30 26 26 37 34 34 33 35 35 35 31 29 33 33 35 33 33 33 23 20 + 29 26 32] +>L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[33 33 33 38 38 38 38 38 35 35 38 38 38 32 34 32 31 11 26 27 20 23 26 38 35 + 38 38 38 38 38 38 37 38 38 33 38 38 36 38 38 38 38 38 38 38 38 36 38 36 38 + 36 38 33 38 36 38 38 38 36 38 23 36 36 37 36 37 36 38 37 33 37 32 35 34 34 + 36 33 35 33 37 33 36 36 36 36 32 36 33 33 33 35 35 27 29 32 23 29 29 32 36 + 36 33 36 31 35 33 33 23 35 33 31 33 15 29 24 8 29 29 31 28 23 31 29 25 18 + 25 23 31] +>L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[27 31 33 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 35 38 38 38 38 38 36 36 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 36 38 38 38 38 38 38 33 36 38 38 38 38 38 36 38 38 38 38 38 38 38 38 33 38 + 17 38 33 38 35 38 38 35 36 38 35 38 37 36 38 37 38 36 38 37 37 35 36 36 38 + 35 36 38 36 37 35 36 38 35 35 33 35 37 34 23 37 34 33 35 35 34 34 35 35 33 + 37 33 34 20 32 32 29 32 29 32 27 32 32] +>L1S208_46 HWI-EAS440_0386:1:25:5972:3048#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 + 40 39 40 40 40 40 31 40 40 40 35 40 40 40 40 40 40 40 36 39 40 39 40 39 40 + 40 39 40 40 40 40 40 37 39 40 40 39 38 40 38 39 36 40 40 40 37 39 40 38 39 + 38 35 39 39 39 34 38 36 34 34 35 36 38 39 35 36 35 38 36 33 33 33 36 35 38 + 35 33 38 34 34 34 30 32 31 28 33 31 31 23 33 31 33] +>L1S140_47 HWI-EAS440_0386:1:25:9861:3050#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 38 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 36 39 34 35 + 31 38 35 39 33 39 39 39 39 36 38 38 36 36 32 34 36 31 36 34 36 34 34 33 35 + 33 27 35 28 35 31 31 32 29 23 32 32 32 32 20 31 23 24 30] +>L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 38 40 40 40 39 39 40 40 40 40 40 40 40 40 40 40 40 + 36 40 40 40 36 40 40 40 40 40 39 40 40 39 39 39 39 39 39 39 39 40 40 38 39 + 36 39 40 40 40 39 40 39 37 39 40 34 38 39 36 37 36 40 36 40 39 36 39 31 28 + 33 35 31 35 36 37 36 34 33 25 33 33 33 34 33 34 37 34 33 33 34 28 34 34 34 + 31 23 36 31 34 33 33 34 33 33 33 33 33 35 23 35 31 26 32 32 31 30 30 33 35 + 35 36 38 35 33 32 32 35 28 29 31 31 31] +>L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[39 39 39 39 39 39 39 39 35 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 37 36 + 39 39 39 39 36 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 38 38 39 38 + 39 38 35 39 39 39 37 38 38 38 39 39 39 39 39 39 37 39 38 39 38 39 38 34 36 + 36 17 36 33 27 32 35 31 36 36 36 33 33 36 38 32 38 36 34 36 33 33 23 16 30 + 26 30 30] +>L1S105_50 HWI-EAS440_0386:1:25:9110:3081#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 39 40 38 + 39 40 40 40 39 40 40 40 40 40 39 40 40 38 39 40 40 40 40 39 40 40 40 39 40 + 40 38 40 40 40 39 40 39 40 40 40 39 39 40 38 40 39 40 39 40 40 38 40 35 34 + 33 40 36 37 39 39 37 39 35 38 39 38 36 35 33 36 38 34 36 35 34 35 36 34 33 + 33 33 36 31 35 35 31 33 32 29 32 30 34 33 31 33 31 31 33 35 33 33 33 28 33 + 31 28 33 33 33 33 33 33 35 22 33 33 33] +>L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[38 38 38 38 38 38 38 38 35 38 38 38 38 39 39 39 39 39 39 37 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 36 39 39 39 + 39 39 39 39 39 36 39 37 36 37 33 38 38 39 38 38 39 39 33 36 38 36 38 37 39 + 33 36 39 39 33 39 38 39 39 35 39 39 39 38 39 38 33 39 34 23 29 23 30 36 34 + 35 36 36 36 36 34 27 33 34 36 36 33 31 24 16 26 28 28 33 33 25 33 31 33 33 + 29 33 31 14 25 25 25 26 20 28 28 29 29 32] +>L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 39 + 39 40 40 40 40 40 40 40 40 40 39 40 40 40 40 37 38 39 39 39 40 38 40 38 39 + 39 40 40 40 40 40 40 39 40 40 39 39 40 40 38 37 37 40 39 39 39 38 40 34 39 + 38 39 38 39 38 38 39 36 35 39 39 38 35 37 38 38 38 38 38 38 35 33 31 33 34 + 34 33 35 32 33 36 31 36 31 33 33 33 33 36 36 33 37 31 30 32 29 30 28 28 33 + 31 14 28 30 31 30 29 26 31 33 33 31] +>L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 38 39 40 40 40 40 35 40 40 40 40 40 + 38 40 40 40 37 40 40 40 38 40 40 40 40 40 40 40 40 34 38 38 40 40 37 33 35 + 38 38 31 40 37 37 40 37 39 38 39 40 39 35 33 36 34 36 39 38 39 38 37 37 35 + 23 37 34 38 34 38 38 38 38 37 38 36 33 35 36 36 36 33 36 36 38 36 38 33 33 + 29 33 33 31 33 33 34 34 32 28 26 30 30 28 27 30 26 19 29 32 31 28 30 25 29 + 31 30 30 25 31 31 7 31] +>L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 35 40 40 40 40 40 + 39 38 40 40 40 40 40 40 40 40 38 40 40 40 37 40 39 40 40 39 40 40 40 38 40 + 39 37 40 40 39 40 40 39 40 38 39 38 38 39 36 39 27 39 38 39 38 36 37 33 34 + 29 36 33 36 36 36 37 34 38 36 38 35 33 36 36 34 36 33 33 34 34 27 31 33 35 + 33 33 36 35 33 35 33 35 31 33 34 33 34 35 35 35 32 35 33 31 28 28 31 30 31 + 33 28 31 31 33 18 33 28 31 31] +>L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[39 39 39 39 39 39 39 39 39 39 35 39 37 39 39 39 39 39 39 39 39 39 39 39 39 + 38 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 38 + 38 38 29 39 39 39 39 39 36 35 38 35 35 38 37 38 38 38 35 38 38 33 38 36 36 + 38 33 38 38 23 38 31 35 36 38 38 36 37 36 37 36 27 38 38 36 36 37 36 38 38 + 39 38 18 35 38 33 33 33 35 35 38 35 31 38 37 35 36 36 35 33 35 33 33 29 33 + 33 33 35 33 33 35 31 29 30 25 26 30 30 28 30 33] +>L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[39 39 39 38 38 38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 39 + 39 39 39 38 38 16 35 27 30 32 26 28 26 38 37 38 33 38 38 27 38 35 38 35 35 + 38 38 33 32 32 35 38 31 39 39 33 39 37 38 39 36 38 36 27 30 28 23 23 35 35 + 35 32 35 31 31 33 33 17 25 26 30 31 30 33 29 29 35 26 32 24 32 23 34] +>L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[39 39 39 39 31 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 38 39 + 39 39 39 39 39 39 39 39 39 39 39 38 36 39 39 39 37 39 36 38 36 35 38 35 35 + 35 38 38 37 37 37 35 35 32 33 36 36 36 35 35 34 37 38 36 36 35 33 34 32 36 + 33 36 36 36 36 36 31 36 33 33 36 33 33 36 36 36 36 23 33 31 31 25 27 23 28 + 28 27 28 33 32 32 29 32 31 25 31 23 31] +>L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 + 39 40 40 40 40 40 40 40 40 40 40 40 39 40 39 37 40 40 39 40 40 39 40 39 39 + 39 40 40 40 39 39 40 39 39 40 40 40 39 40 38 40 39 40 37 39 40 37 39 36 35 + 33 40 35 37 37 36 39 39 38 38 39 39 36 37 36 33 40 36 37 37 34 36 36 35 29 + 36 35 36 33 36 36 35 33 36 36 39 35 33 36 37 36 33 35 38 36 36 35 35 33 37 + 36 35 36 33 34 33 33 33 26 34 33 33 33] +>L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 + 39 40 40 40 37 40 40 40 39 40 39 40 40 39 39 40 39 39 40 40 40 40 40 39 37 + 40 40 38 40 37 35 32 38 40 40 39 37 39 39 36 39 31 40 39 40 40 38 40 39 38 + 38 40 36 39 38 39 36 39 38 39 37 39 38 36 36 36 40 39 39 36 36 36 31 36 36 + 36 36 40 36 37 39 33 36 35 37 38 35 37 38 38 36 36 35 36 36 33 33 16 23 32 + 31 31 31 33 31 23 23 31] +>L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 39 40 40 40 40 40 40 40 40 40 40 40 39 40 38 40 40 38 40 40 40 40 38 40 40 + 40 38 40 40 40 39 40 40 40 40 40 39 39 39 40 40 40 39 40 40 40 39 40 39 39 + 40 27 39 37 40 37 39 36 40 39 36 39 38 38 39 34 33 39 33 38 27 35 35 35 32 + 30 31 32 35 31 23 28 33 32 32 30 27 21 30 32 32 30 30 16 27 27 24 26 17 20 + 19 21 27 30 10 25 31] +>L1S76_61 HWI-EAS440_0386:1:25:3312:3259#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 25 35 38 38 38 38 37 38 40 40 40 40 39 40 40 40 40 39 + 37 40 40 40 35 40 40 40 40 40 39 40 40 40 39 35 40 40 40 34 40 40 40 36 39 + 40 39 40 40 40 39 40 40 40 39 40 40 39 37 36 36 29 40 40 39 39 38 40 38 36 + 29 37 36 36 36 37 39 36 36 38 38 38 38 34 39 36 39 38 38 36 38 35 36 36 38 + 38 31 38 35 35 36 35 38 36 33 36 38 35 33 36 27 36 33 36 33 33 33 34 31 31 + 31] +>L1S57_62 HWI-EAS440_0386:1:25:3550:3291#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 39 40 40 35 40 40 40 40 40 40 36 40 40 40 40 39 + 37 39 40 40 40 39 40 40 40 40 39 37 40 40 40 39 40 40 40 40 40 40 40 36 39 + 38 40 35 40 40 35 40 38 40 37 37 33 40 40 39 36 35 40 36 36 36 37 38 36 39 + 36 40 33 37 34 37 35 36 38 39 38 38 38 39 38 39 38 36 29 36 36 35 36 33 36 + 33 33 35 35 34 34 35 35 34 29 35 34 34 31 34 30 34 33 34 36 31 33 33 33 33 + 29 24 31 27 29 30 26 31 10 26 27 30] +>L1S257_63 HWI-EAS440_0386:1:25:5478:3298#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 + 39 40 40 40 39 40 40 40 40 40 39 40 40 40 40 40 39 39 39 39 40 40 40 39 39 + 40 40 38 40 39 40 40 40 39 39 40 40 40 39 34 40 39 40 37 39 40 37 37 36 36 + 31 39 35 39 38 39 37 38 38 38 38 38 36 38 36 36 39 36 38 39 36 34 38 36 36 + 38 38 39 33 33 35 35 33 33 23 33 31 33 31 31 32] +>L1S281_64 HWI-EAS440_0386:1:25:15346:3296#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 17 38 38 + 35 38 38 40 39 40 40 40 40 39 40 40 40 40 39 40 39 40 40 36 40 40 40 40 37 + 40 40 40 40 40 39 40 40 40 40 40 40 38 40 37 37 35 40 40 40 40 39 40 36 36 + 35 40 36 40 36 37 39 39 33 36 40 34 36 33 35 34 36 33 36 34 35 36 36 36 39 + 36 33 37 33 34 36 33 35 33 28 33 33 35 30 32 32 30] +>L1S76_65 HWI-EAS440_0386:1:25:3468:3322#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 39 40 40 40 38 38 38 38 38 40 39 40 40 40 40 39 33 40 40 40 39 + 40 40 40 40 40 40 40 40 40 38 40 40 39 39 39 39 40 40 40 38 40 40 35 40 39 + 40 40 40 40 39 39 40 37 36 40 36 36 36 37 35 34 37 37 38 36 38 35 39 38 38 + 36 38 38 38 38 39 38 36 36 35 38 38 36 36 35 35 38 37 23 36 33 33 34 35 36 + 36 35 33 33 36 31 31 31 33 34 33 34 33 33 31 36 33 33 33 33 31 28 28 19 31 + 30 30 18 28 28 28 24 24 31 16 33 31] +>L1S281_66 HWI-EAS440_0386:1:25:4199:3321#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 38 40 40 40 38 40 40 40 40 40 40 40 40 40 40 39 + 35 40 40 40 40 40 40 40 40 40 39 40 40 38 38 36 38 38 40 39 40 40 40 40 40 + 39 40 36 40 40 40 36 40 40 40 39 38 40 40 39 40 40 38 40 38 40 36 40 39 39 + 40 39 39 39 38 36 38 36 34 27 36 39 34 38 38 39 36 37 36 27 36 33 31 33 33 + 36 29 34 34 36 33 29 33 26 14 28 30 28 33 34 33 34 33 29 33 33 29 31 31 33 + 27 31 30 27 23 20 9 24 17 23 20 22 23 28 31 33] +>L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 38 39 39 39 39 39 39 39 38 39 39 39 39 39 39 37 39 39 39 39 39 + 37 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 36 39 39 39 36 36 + 38 35 38 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 35 38 + 38 39 38 39 39 39 39 37 36 39 38 38 33 39 33 36 39 36 38 39 38 39 38 38 39 + 38 35 39 32 36 39 36 39 33 33 35 33 33 33 33 34 29 33 36 36 36 33 33 32 36 + 32 32 36 36 33 36 32 33 29 27 31 33] +>L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[39 39 39 39 39 39 39 39 36 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 + 39 39 39 39 35 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 35 39 39 39 39 36 37 39 39 39 39 39 31 39 39 39 39 39 36 39 39 36 38 36 36 + 36 39 35 39 36 37 39 36 38 39 36 31 36 39 38 38 39 38 34 31 33 23 35 36 36 + 36 34 36 33 36 36 35 23 26 30 28 30 30 35 33 34 35 33 34 34 34 33 35 33 33 + 27 33 33 33 34 36 23 36 18 28 18 28 30] +>L1S140_69 HWI-EAS440_0386:1:25:4994:3354#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 37 39 39 39 39 38 38 + 39 37 39 39 39 39 39 39 39 39 39 39 39 39 38 39 38 39 36 39 39 36 39 35 29 + 34 36 34 38 38 39 39 31 38 38 34 37 38 38 36 38 39 38 33 31 33 33 33 36 36 + 36 36 39 31 33 33 34 34 33 31 33 29 34 36 27 34 31 33 33 36 33 33 32 35 36 + 32 32 29 28 32 31 31 28 25 28 28 30] +>L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[36 36 36 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 38 35 38 38 38 38 38 38 38 38 38 38 37 38 37 38 38 38 38 35 38 37 38 38 37 + 29 36 38 36 38 36 37 36 35 23 35 38 35 36 36 36 33 38 35 38 33 35 37 35 33 + 35 33 35 36 36 27 33 36 35 31 31 32 31 23 33 26 28 24 29 23 29 10 29 26 26 + 19 24 26 30] +>L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 39 40 35 40 40 40 40 40 35 40 40 40 40 40 40 40 40 40 40 38 + 35 40 40 40 36 40 40 40 40 40 40 40 40 37 37 37 40 40 40 39 40 40 40 39 36 + 40 40 40 40 38 37 40 40 40 38 40 40 39 40 36 39 36 40 37 35 40 37 40 38 38 + 38 39 36 40 36 35 39 34 38 39 38 39 39 35 36 36 40 36 36 36 36 36 36 38 38 + 38 29 38 35 36 35 33 33 29 32 35 32 35 31 32 26 29 19 27 28 27 24 21 22 25 + 27 27 28 25 30] +>L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[39 39 39 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 39 39 39 39 39 39 + 39 38 39 39 39 39 39 39 39 39 36 39 39 38 38 33 38 38 38 39 39 39 39 39 39 + 39 39 39 39 38 39 39 39 39 39 39 38 36 38 37 37 38 37 37 38 37 35 37 36 37 + 39 37 35 39 36 36 39 33 34 17 33 36 34 23 28 30 30 32 33 33 33 31 35 31 31 + 29 33 33 33 33 31 20 31 22 22 28 28 16 34 34 31 33 33] +>L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[38 38 38 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 38 40 40 40 40 40 + 35 40 40 40 38 40 40 40 40 40 40 40 39 40 40 40 38 40 40 38 40 40 39 36 38 + 38 38 34 40 40 37 40 40 40 39 40 40 40 40 39 38 39 40 39 39 40 36 39 39 36 + 35 35 39 37 33 40 35 37 37 39 39 37 36 38 38 38 39 38 29 36 36 33 36 33 34 + 36 33 34 31 33 33 33 33 28 21 24 28 11 25 16 23 21 25 36 33 36 33 34 26 26 + 31 25 31] +>L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 39 40 40 40 40 40 40 39 + 39 40 40 40 40 40 40 40 40 40 39 40 40 39 39 40 40 40 40 39 40 40 40 39 38 + 39 39 40 40 40 39 39 40 39 40 40 40 39 40 36 36 36 40 39 40 40 38 40 37 34 + 35 40 33 39 39 40 31 39 39 38 36 35 35 29 33 31 35 33 36 33 33 33 36 36 36 + 33 36 37 34 36 38 36 36 33 27 35 33 33 36 36 33 36 33 31 33 33 27 34 28 33 + 22 31 33 31 34 33 31] +>L1S105_75 HWI-EAS440_0386:1:25:9796:3444#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 35 40 40 40 40 40 40 40 39 40 39 40 40 38 40 40 38 40 40 39 40 40 40 40 40 + 40 39 39 40 33 40 40 40 40 40 40 40 38 40 36 40 39 38 40 40 40 36 39 40 40 + 31 40 38 40 39 37 35 39 40 38 35 40 39 39 37 36 36 40 38 33 33 27 35 35 37 + 37 37 37 36 37 35 36 33 33 35 38 38 33 37 34 36 36 34 35 35 35 31 35 31 37 + 33 33 35 35 26 33 33 32 32 31 35 24 35 25 25 30 18 28 27 21 20 23 20 20 26 + 25 27] +>L1S8_76 HWI-EAS440_0386:1:25:2235:3462#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[39 39 39 38 36 38 38 38 37 38 33 38 38 39 39 39 39 39 39 37 34 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 35 39 36 37 37 37 38 31 36 36 36 37 27 39 37 + 39 39 39 39 35 39 37 23 35 39 33 39 39 39 32 38 39 38 37 37 35 32 35 35 35 + 37 34 33 36 36 35 35 32 34 36 33 36 33 36 33 22 29 36 29 29 8 29 31 36 35 + 34 34 31 33 27 29 25 20 25 21 25 29 21 23 29 15 32 26 24 29 26 25 31 30 27 + 29 30 30 31 33] +>L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 38 39 39 39 39 39 + 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 + 39 39 39 39 38 33 39 39 39 39 39 33 39 39 39 39 39 39 39 37 39 38 39 33 39 + 39 37 38 36 38 36 36 33 36 39 39 39 35 35 35 33 31 38 33 28 30 28 30 36 36 + 36 29 36 33 35 18 28 31 32 32 33 35 35 35 29 33 33 34 32 27 32 28 32] +>L1S281_78 HWI-EAS440_0386:1:25:13919:3507#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 39 40 40 37 40 40 40 38 38 39 40 40 39 40 35 40 39 40 40 38 + 33 40 40 40 36 40 40 40 39 39 36 40 40 39 36 38 39 39 39 36 39 38 40 35 33 + 36 38 33 40 37 36 37 37 36 36 39 36 33 39 36 37 34 39 38 38 39 36 39 36 31 + 33 39 33 37 35 37 36 36 38 36 35 35 36 33 33 33 39 36 31 35 33 33 35 33 36 + 33 33 36 35 36 36 35 33 36 29 32 36 34 35 34 35 33 31 33 33 31 25 34 31 33 + 33 31 33 28 15 28 15 29 30 28 29 31] +>L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 39 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 + 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 39 40 37 40 40 40 40 40 39 39 + 40 37 40 40 40 39 38 39 39 40 39 40 40 40 39 39 40 40 37 37 40 34 40 33 23 + 33 35 38 35 31 35 33 32 34 37 36 35 38 34 36 36 33 37 37 37 34 33 33 33 36 + 33 36 33 32 32 26 23 30 29 30 32 32 32 33 33 34 34 31 33 33 29 29 34 31 35 + 33 31 33 31 22 31 31 31 23 18 26 23 31 23 24 27 22 10 28 23 28 7 28 20 30 + 24 30] +>L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[27 31 31 38 38 38 38 38 38 38 33 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 38 38 38 38 38 38 35 36 38 38 38 38 38 38 38 38 38 38 38 33 38 38 38 38 38 + 38 35 38 38 38 33 27 33 38 38 38 38 38 38 38 38 38 38 29 27 38 38 38 35 38 + 35 38 38 38 36 38 35 38 38 38 38 38 36 38 37 36 27 35 38 35 35 35 35 38 38 + 38 33 38 35 35 38 35 25 33 33 35 35 35 30 30 25 27 30 38 38 35 33 33 26 33] +>L1S76_81 HWI-EAS440_0386:1:25:11581:3662#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 38 40 40 40 40 38 39 40 40 38 38 40 40 40 40 40 39 40 40 40 40 39 + 40 39 40 40 40 39 40 40 40 40 36 40 36 40 37 37 38 38 38 40 37 40 39 40 40 + 39 37 40 39 40 39 35 37 40 40 40 38 36 39 36 39 39 40 39 40 38 38 37 36 38 + 38 27 39 36 38 33 35 29 31 29 32 35 32 36 36 36 31 35 30 32 28 27 28 31 33 + 31 31 31 33 31 33 29 33 33 33 33 34 32 31 31 8 29 31 27 19 29 16 29 31 31 + 29 26 29 32 19 25 31 32] +>L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 + 35 40 40 40 40 40 40 40 40 40 39 40 40 39 40 40 40 40 40 39 40 40 40 39 35 + 40 37 40 40 40 40 40 40 40 40 40 40 39 40 38 40 38 40 39 39 40 36 40 37 37 + 36 40 36 40 39 40 37 40 38 38 39 38 39 35 39 39 39 38 39 38 33 27 36 36 35 + 39 36 37 38 38 39 33 38 36 35 39 39 39 33 34 33 36 33 36 36 35 31 33 23 31 + 31 31 32 31 33 31 31 31 23 30 18 30] +>L1S208_83 HWI-EAS440_0386:1:25:8197:3691#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 39 + 38 40 40 40 39 40 40 40 40 40 40 40 40 40 39 39 40 40 40 40 40 38 37 40 40 + 40 40 40 40 40 40 40 40 40 40 40 39 38 40 37 37 38 40 37 40 40 37 40 38 39 + 33 40 39 38 40 39 40 38 39 38 38 36 35 35 33 35 33 37 35 29 33 31 31 33 33 + 33 28 33 33 35 33 30 31 33 25 33 33 33 36 36 36 34 33 30 30 32 19 31 19 30 + 28 28 30] +>L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[28 33 31 38 35 38 38 38 36 38 36 38 38 38 38 38 38 38 38 36 38 38 38 38 38 + 35 38 38 38 38 36 36 36 38 38 38 33 38 38 38 38 38 38 38 35 38 38 38 38 38 + 38 38 31 36 36 33 33 36 30 30 30 35 33 38 38 38 36 38 33 38 38 35 38 38 38 + 38 33 34 30 15 30 27 33 37 38 37 29 33 35 38 35 35 38 34 33 35 35 35 35 35 + 35 35 35 36 38 38 37 35 35 35 32 38 10 7 17 25 18 24 35 33 32 32 32] +>L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 37 + 37 40 40 40 37 40 40 40 40 40 39 40 40 40 39 36 40 40 40 39 40 40 40 39 39 + 40 37 40 40 40 40 39 40 40 40 39 39 39 40 38 39 39 37 39 40 39 36 40 35 33 + 33 37 38 39 37 39 37 36 39 36 40 40 36 33 35 33 36 36 35 34 35 36 37 28 35 + 33 35 35 34 33 35 28 35 31 33 35 36 33 35 37 34 34 29 33 31 33 19 33 28 31 + 28 29 31 28 33 33 29 23 30]
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_fastq_libraries_sequences.fasta Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,172 @@ +>L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGATGTGAAAGACATGGGCTCAACCTGTGGACTGCATTGGAAACTGTCATA +>L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTG +>L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTA +>L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAG +>L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG +>L1S257_5 HWI-EAS440_0386:1:25:2639:1894#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG +>L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGT +>L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG +>L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCGACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAG +>L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTT +>L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAG +>L1S76_11 HWI-EAS440_0386:1:25:6540:2228#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTA +>L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTT +>L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG +>L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAG +>L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAG +>L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAA +>L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGG +>L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG +>L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGG +>L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG +>L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG +>L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCG +>L1S76_25 HWI-EAS440_0386:1:25:8159:2639#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTA +>L1S140_26 HWI-EAS440_0386:1:25:11175:2690#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAGGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGC +>L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG +>L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCG +>L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA +>L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG +>L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTA +>L1S140_32 HWI-EAS440_0386:1:25:14127:2819#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTT +>L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTGGTGAAAGATCGAAGCTTAACTTCGGTAAGCCATGGAAACCGTAGGGCT +>L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAG +>L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCGACCGTAAAATTGCAGTTGATACTGGCTG +>L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAG +>L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTC +>L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATAACTTGAGTGCAG +>L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTG +>L1S76_40 HWI-EAS440_0386:1:25:6132:2959#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCAGGGCTCAACCCCGGGACTGCTTTTGAAACTGCAGGGCTAGAGTGCAGGAGGGGCAAGTGG +>L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGG +>L1S57_42 HWI-EAS440_0386:1:25:1831:2983#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGC +>L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGCTGAAACTGGCAGTCTTGAGTACAG +>L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGGTGCAAGCGATAATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGGGTGAAATCCCACAGCCCAACTGTGGAACTGCCTTTGATACTGCCAGGCTTGAGTACCG +>L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG +>L1S208_46 HWI-EAS440_0386:1:25:5972:3048#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGA +>L1S140_47 HWI-EAS440_0386:1:25:9861:3050#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCT +>L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG +>L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTG +>L1S105_50 HWI-EAS440_0386:1:25:9110:3081#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG +>L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGG +>L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG +>L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG +>L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGC +>L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGG +>L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAG +>L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG +>L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGG +>L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAG +>L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGG +>L1S76_61 HWI-EAS440_0386:1:25:3312:3259#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGC +>L1S57_62 HWI-EAS440_0386:1:25:3550:3291#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG +>L1S257_63 HWI-EAS440_0386:1:25:5478:3298#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG +>L1S281_64 HWI-EAS440_0386:1:25:15346:3296#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCTGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGT +>L1S76_65 HWI-EAS440_0386:1:25:3468:3322#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGCTGTGCAAGACAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGTGACTGCACGGCTAGAGTTTGTCAGAGGAGG +>L1S281_66 HWI-EAS440_0386:1:25:4199:3321#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCATCACAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTTGAAACTGTGGAGCTGGAGTGCAGGAGAGGCAAGTGG +>L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG +>L1S140_69 HWI-EAS440_0386:1:25:4994:3354#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGG +>L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTT +>L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGGAC +>L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAG +>L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA +>L1S105_75 HWI-EAS440_0386:1:25:9796:3444#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAAGTGAAAATCCGGGGCTCAACCCCGGAACTGCTTTTGAAACTGTGAGGCTAGAGTGCAGGAGGGGTGAGTGGAATTCCTAGTG +>L1S8_76 HWI-EAS440_0386:1:25:2235:3462#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAGTCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCA +>L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAG +>L1S281_78 HWI-EAS440_0386:1:25:13919:3507#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGG +>L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAAGTGAAATGCGGGGGCTCAACCCCTGAACTGCTTTGGAAACTGTCATGCTAGAGTGCAGGAGGGGTGAGCGGAATTCCTAGTG +>L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTAATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTG +>L1S76_81 HWI-EAS440_0386:1:25:11581:3662#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGG +>L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAGTTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG +>L1S208_83 HWI-EAS440_0386:1:25:8197:3691#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGAGTGTCG +>L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAG +>L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCGGTGAAATTTTGGTGCTTAACACCAAACGTGCCGTTGATACTGCTGGGCTAGAGAGTAGTTGCGG
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_libraries_fastq_histograms.txt Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,8 @@ +Length Count +116.0 20 +126.0 31 +136.0 33 +146.0 2 +156.0 0 +-- +
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_libraries_fastq_seqs.fastq Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,344 @@ +@L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGATGTGAAAGACATGGGCTCAACCTGTGGACTGCATTGGAAACTGTCATA ++ +ACCCGGGG@G?GGGGGGGGDGGGGGGGGGGGGGGGGDGGBGGGBGGGGGGGDGEGGDDG@GGGGEEEGGGGGG388GD>DDGGEE@DDDDB>FDGD>@-:74=;A*74====?BBD@ +@L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTG ++ +GGGGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHFHBHHECCHBDHFEHAE@@BDB=B@@A2D(5553DAAAAE@EEA>;)9;==A>7B@=@78;:82?C<><9?<>>>98=@ +@L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTA ++ +IIIGIIIIGIIIIIGIIHIDIHIHHF@IIFIIIIIGIIIHIHGHBIIIGHHGIFEEEFHHHDEEDG>DBEFBDB@BB=EEBBBEGEDDBA>D@@@@BCBB;BD@@>:@@>B@?=1@@@3<9767=6>;;? +@L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAG ++ +IIIIIGIIIIIIIGIIIIIIIIIIIFIIGEGGDIIIIIFFHIIGDGEGD>GDGIIEIGIIFH>GBDDGFGGEGBB2DBDDGBDEBEBB8?<B>B>A@D=38=AC>?>@@?DD@>=?=478;+;BE8>B +@L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG ++ +IIIIIHIIIIIIHEEIIEIIIIIIGGHIHEIIHDFCIGHHHCEDBIEIEEHBBEBBEIHGGGGGDHGGEEGDG?<2A5DBBBBEBEBBB@BEDEBBBE;@@@B@@8:@B@@@@AE@@A0@>=<9:;9=7>:;==<? +@L1S257_5 HWI-EAS440_0386:1:25:2639:1894#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG ++ +HHHGGEGEGGGGGHHHHHHHGHHGGCGGHFHHHHHHHHEDBGGHFHHHHFHHHHHFHHHHHEBFCEEDFFFDE=;@C=CCCAABBD@@:=;?=BB@>DBD=@DBBB=D6'=;;A=?=99=9.9.2,/2===79@@6@ +@L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGT ++ +IIIIIIIIGIIIDGIIIFIIHIIIIIIIIIIIIIIEIFGFGGGHIIGIIIHBHIHGIGIHI@EG@EGFFIFEDFBC<FEC@B+@BBBB82==?BB@<BB@BB)AA@8=@?@@5?<>=;;2?-?=A? +@L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIBIIIGIIIIIIIIIIFIIIIIIIIIIIHGIIIIIIIEDGFGEDDGDBG:@@ECGEFEBHBHFHBB@@GGBBBDBECBBGECBECBFEBDEBEBB@@2B4?<=?;A??A@A+>? +@L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCGACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAG ++ +IIIIIIIIIIIIHGGIIHIIIIIIIHIIIHIIIIIGIHHIIIIHDIIIHHHDIIIHIIHDHGEHEHGDGHEDGDHEEFEHG,EDCDCF@EBBECB@@B6>@A@ECBBCBBEEA>BBB@@DBAB=@8@@ +@L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTT ++ +GGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHGHHHHGBHHHHHHHHHFHHHHEHHHHEFEHFGGFGAA>C=GGDBDEDEEBCBBEEBB>BD@BB@DBBBB=C>BCCB>EBB8? +@L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAG ++ +HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGBGGGEGGGG@GGDGHHFGHHHHHHGGFEGBGGG3CE>EAABGDBFFEDFE>E>DCB@EFFBC>EDBDD+CAA>>9?=99DCC?ACABEBBEB<BBD@B@@@B +@L1S76_11 HWI-EAS440_0386:1:25:6540:2228#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTA ++ +IIIIIIIIHIIIIIIIIIIIIIIHIIIIIIIIIIIHIIGIIGIIIIHIIIIDGIBFHDHBEHHIEDHGHHIFFGGH@>EECEBGDECDEEEEEED8B>@@@??EEB*B71;2>7@?===@ +@L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTT ++ +IIIIIIIIHIIIHIIIIIIIIIIIIBIIIFIIIIIHIIHHHIIFFIIIFEIFIIIHIIIIIHGIEECHEFIDIBC>GBFFFEEHBEGEBBEFDBCBBF8A??ABBD=B@@DBB??A;;>? +@L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG ++ +IIIIIIIHIIIIIIIIIHIIIIIGIFIIIIIIIIIHIIIHHIHHFIIIGIIIEGIFHIIGHIIIGHGIGIGHIEDBFAEHHFEGGDGE<CBFCGDEGGGGEGHEECE>CBC@BDB@ +@L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAG ++ +HHHHHHHHHHHHHHHHHHHHHHHHHDHHHHHHHGHHHHHHHHHGFGGGFGGEGGGGGGHHHHHGHHHHHHHHHEEEGCHHHGGGHHHBHGH>EEDBDEHHHDFBFG><??CAC8DDABDEEBEDB@BEB@B>B +@L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ++ +IIIIIIIIIIIIGIIIIIIIIIIHIEIIIHIIIGIIIIIIHIHFIIIIIDIHHIGGIIIIHBHIFIFIIIHHIEE@GBGDDDD@EHEEEECBEEEDCFBCBBFEEI>@B@DBDBEEB9???,?<A>@A=A=<=;*;? +@L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAG ++ +IIIIIIIIIIIIGIIIIIIDIIIIIHIIIHIIIIIHIIHHIIIIHIIIHHFHGIHHIGGIEHDICDEIEIIEIBBBD@FBEEBEEEFDDBEFEBBBBEBBBBD?BB88<:<9?B@B@@@A?3@@@9?@ +@L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAA ++ +HHHHHHHHDAGGGHHHHHHHHHHHHHHHHHHHHHGHHHHHHH@HHHHGHHFGHHHHHHHFHHHHGHHHDHHGHGGH<GE@DEBEFEFHGGGBG@BBEDBDB@DEBFEEGEE>GBC>DECEDB>6>>>A6>>;4>=6:=9=B:@ +@L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGG ++ +GGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEHHHHHHHFHHHEHEHHHHHHHBHHEHGHHHFGGGGBEEHEGCBGDCGEGGEGGEGECCEC@EE>B@A==@7,8=>;1=9A>>>;A +@L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ++ +IIIIIIIIIIGIHHIIIIIGIIIIIFIIIHIIIIIHIIIHIIGIGIIIHIIFIIIGIIIDHGDIFIFEHIFBHBDFFFEIE8BDEDDEEFDDHECC@EABCEEBAD3D@BCCCEF>CBBBC8C?B@=@@<<>BB=@@B +@L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGG ++ +GGGHHHHHGEGGGHHHHHHHHHHHHHHHHHGHHHHHBHHHHHHGCGBGEEGEEGGDBBADBGDFDEEGCGFEFFCDBBDBECCEFHEEEE:HB;A<::=?>=;EEEAE@>AC>,:.52?;:5255:,7@3:4:@ +@L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG ++ +IIIGIIIIIIIIGIIIIIIGIGIIHIIIFIHIIIIHHIIIIIEIEIHIDDGG@GGBGGGHIIIIGIFIGIIHHEEEGBHEHHFFGHGGGGEGDEBEDEACDDCABBDDB@@@BEBEBCCBDBBBBDEE?B@>B +@L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ++ +GGGHHHHHBHHHHHHHHHHHHHHHHHHHHHGHHHHHGHHHHHBGHHHHHGHHBGGEGEDGBGGHHH>HDBGDGHHGHED>GB@GEGEGHFBHED@BDDDHFD@C>FCEFEHBB>@?:>??8826=8/:;<<:?>)>A +@L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG ++ +IIIIIIIIIIIIBHHIIHIHIIIIICIIIFIIIIIHIIIHGHHFEIHIEFIIBIHFHIIHIIGHEHGHGHHGHDBBICHEFHEGGDGEECGHEGDE>GGEDEGDDDBEEBEE@BBEBBDGDC=ABBBB?==-:==? +@L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCG ++ +HIIIIIIIIIIIIIIIIIIIIIIIIIDIII3IIGGGGGIIIII@HIIGIIHGIIIIIHIIHIIIHIIDEGGEGGHHIHIHIIGDFIBF<DBDDBIDHHIIGGGFIFEHAACEAEIDI9IIFCFFEEEF<BB<D==???<@ +@L1S76_25 HWI-EAS440_0386:1:25:8159:2639#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTA ++ +IIIIIIIIIIIIIIIIIHIGIIIIIGIIIHIIIIIGIHHHHHHIHIHIDEIGCIFHHIHEIHEHEHEIEIHEIBDBFBFCDDDGEGEEDDDGCBDBBDABEBEDGDEAEBCBCBBEBB@EB7BB@BBB6? +@L1S140_26 HWI-EAS440_0386:1:25:11175:2690#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAGGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGC ++ +CCCGBGGGEGGGGGGGEGGGGGGGGDGGGGGEDGGFGGGGGGGGFDDGFGGEGGGBEGEGDGGGFG>GFGGFGFEEGEFEF@EEEEFEBEDFDCD@BBEDE>EDCFBEDBEF>;A>:>AA8>>;AA +@L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG ++ +IIIIIIIIIIIIFIHIIIIGIIIIIFIIIHIIIIIIIIIIDIHIIIHIGIIHIIIIIIIIFHHIGHGIHIGEDBBBGBGHFHGCGHGEBEEIEEBEECEEHCHBEFBBB@BBBC>E@B?@@?@=@@BB;>@?>@?>A +@L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCG ++ +DGGEBEEE?B,B?GGGGGGGGGGGGGGGGG>GGDGGGGGGGGGGGGGGGGGGGGGA>>G@>G<E?BEE@GGDGGDGBDGEB>DDGDGFFGD8FBB??>GDGDBA@AD;AAAE5?B;;4ADDBBB@B@D1.?8??-8::;? +@L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA ++ +IIIIIIIIIIIIHHHIIIIIIIIHIBIIIFIIIIIHIIIIIIEFIIFIEHFHHIIFIIIIHIHIEIHIHIIFICECIBHHIFEHDEGECBBHEDCBDFCEEBHBBEC>B=BBBCEBDA@BB>B>BB@CB=?@ +@L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG ++ +IIIIIIIIHIIIDIIIIIIIIIIIIFIIIHIIIIIHIIIIHIIIIIIIIIIIIIEIIIIIIIGIHHEIHIHHICEHIHHII@EHEHFEGDGHGEFEEFGGBEHBEE<F@@BDDDCBDBDDBBB>BBBEBEB@@>@=@B +@L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTA ++ +IIIIHIHIEGGGEIIIIIIIIIIIIGIIIHIIIEGBGDDDDGGGIIIBEHIIFHHIIFIIGDBC@>EC3@?>@GEGG@@DBD@E8CC=BBDBEAC,38==:;=@?,3=9-<;<?A?? +@L1S140_32 HWI-EAS440_0386:1:25:14127:2819#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTT ++ +GGGGGGGGHHHHHHHHHHHHHHHGHHHHHHHHHGHHHHHHHHHGEHHHHHHHHHHGHHHHHHGEBEEGEGGFGCCBG@EEFDEEEGDEEBBHEDEBEEGGBEGBAEBD@AHDC?CE@?BDB:>68<===B +@L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTGGTGAAAGATCGAAGCTTAACTTCGGTAAGCCATGGAAACCGTAGGGCT ++ +HHHHHHHHEGGGGHHHHHHHHHHHHFHHHHHHHDBGGFHHHHBGGDDDHHHHGGHHBFGFCGGFBEGGDGD>D>9ACACEEEBEEBBCDB@EF>DBB@C3*=@AA@=@BAEEBCCB@C +@L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAG ++ +IIIIIIIIFIIIIIIIIIIIHIIIHHIIIHIIHIIHIIIFHIIFHIIIHFHIIDHHIHIIIIHIEHDIGIHGFDDCFCB@DEBFEFCFC>EDDBBBBBAAA?A@BB@E@?>-@A@B??@DB-A +@L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCGACCGTAAAATTGCAGTTGATACTGGCTG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIIIHIIGHIIIFHHIHIGIIIIHIIIIGHHIHIGHIECCGCFFIFFHDFGB=@@DBBBBBBDB?@EBBB=B>=B@BBBB +@L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAG ++ +HIIIIIIIIIIIIDIIIHIIIIIIIBIIIGIIIIIGIGIHIFIHHIIIGHIIIFHHIGDFIEEIEFIDFHHGEGBHCFHGEGDGCDEBCCBBEEC>B@B>B8@BBB:B;???9@B>C@>;? +@L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTC ++ +IIIIIIIIIHIIIHIIIIIIIIIIIIIIIIIIIIIIIHIHHHIIHIHIEIIFEHHIIIIIIIHIGIHIBDHEHGHHHGHHEGDBCDF>DDCBFE@?BB@B@78BB;@=@@?@=;A=????@:>=@=B +@L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATAACTTGAGTGCAG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIGIIIHIIIIIHIIHHHIIIHIIIEEIFIIIFHIIIHHEIGGGIGIIGIGGDIEGGHGHDEEABCCEDBC@BBEBBDBFEEGABB@BCC<<?'+=><<;:A0?A +@L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTG ++ +IIIIGIIIIIIIIGGIIIIIIIIIIIIIIIIIIDIIIIIIIIHIHHHIIIHFIIHHIGIIIIHGEIDGFIIHIHFEIEHGIGHGGEG@EEBGEEBDEEEDGEHHDGHHGDDCGBDBB?DBD@B??A8A?@D<B7-? +@L1S76_40 HWI-EAS440_0386:1:25:6132:2959#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCAGGGCTCAACCCCGGGACTGCTTTTGAAACTGCAGGGCTAGAGTGCAGGAGGGGCAAGTGG ++ +A>BGGGGGGGDGGGGGGGG@GGGGGGGGGGGGGGGGGGGGGGGDGGDGCFF>?GGGGGGEGGGGGG8GGGGDGGBG<GEG<GGEDEGGGGEEG1=??BEFD>CE>CECDEBEC?>,+?A=??==B@BBA<>>/=9?8?>>? +@L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGG ++ +IIIIIIIIIIIIIFIIIIIGIIIIIBIIIFIIIGIFIIIHHHHIHIIIFHIGEIIHHIHHIH>IFIEIHHGHIBC>GCFEIEEHGEGDEEDGDBCBBBDDEEH@<BBBEBCBEEFEEBEEE@A:?@BB<58'2<<<69;?A +@L1S57_42 HWI-EAS440_0386:1:25:1831:2983#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGC ++ +EFFGGGGGGBDGGHHHHHHHHHHHHGHHHHGHHHHHHHHHHHHHHHGHBGEGGEG@GGGGEFFGECGGCCEACDDD8G;@A@@@BDDBFEHEDB>2@AEEB>BAA>>9>=AA77>>@? +@L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGCTGAAACTGGCAGTCTTGAGTACAG ++ +BEEGGGGG3EBEDEEEEEGG3<GEED<EGG@EDGGB<GEEEEAB.4BB5>@>,EE<EEGGGGDGFGGG>GGADB??<16/459DFDGFFFDB15*9:/AAAAA(??;;FCCBDDD@>BBDBBB85>;A +@L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGGTGCAAGCGATAATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGGGTGAAATCCCACAGCCCAACTGTGGAACTGCCTTTGATACTGCCAGGCTTGAGTACCG ++ +BBBGGGGGDDGGGACA@,;<58;GDGGGGGGFGGBGGEGGGGGGGGEGEGEGBGEGGGEG8EEFEFEGFBFADCCEBDBFBEEEEAEBBBDD<>A8>>AEEBE@DBB8DB@B0>9)>>@=8@>:3:8@ +@L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG ++ +<@BGGGGGGGGGGGGGGGGGGGGGGDGGGGGEEGGGGGGGGGGGGGGGGGEGGGGGGBEGGGGGEGGGGGGGGBG2GBGDGGDEGDGFEGFGEGFFDEEGDEGEFDEGDDBDFC8FCBDDCCDDBFBC5AA>A>A<AA +@L1S208_46 HWI-EAS440_0386:1:25:5972:3048#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGA ++ +IIIIIHIIIIIIIIIIIIIIGIIIIIHIIII@IIIDIIIIIIIEHIHIHIIHIIIIIFHIIHGIGHEIIIFHIGHGDHHHCGECCDEGHDEDGEBBBEDGDBGCCC?A@=B@@8B@B +@L1S140_47 HWI-EAS440_0386:1:25:9861:3050#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCT ++ +GGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHHHHEHCD@GDHBHHHHEGGEEACE@ECECCBDB<D=D@@A>8AAAA5@89? +@L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG ++ +IIIIIIIIGIIIHHIIIIIIIIIIIEIIIEIIIIIHIIHHHHHHHHIIGHEHIIIHIHFHICGHEFEIEIHEH@=BD@DEFECB:BBBCBCFCBBC=CCC@8E@CBBCBBBBBD8D@;AA@??BDDEGDBAAD=>@@@ +@L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTG ++ +HHHHHHHHDGGGGHHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHHFEHHHHEHHHHHHHHHHHDHHHHGGHGHGDHHHFGGGHHHHHHFHGHGHGCEE2EB<AD@EEEBBEGAGECEBB81?;?? +@L1S105_50 HWI-EAS440_0386:1:25:9110:3081#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG ++ +IIIIIIIIIIIIIGIIIIIIIIHIGHIIIHIIIIIHIIGHIIIIHIIIHIIGIIIHIHIIIHHIGIHIHIIGIDCBIEFHHFHDGHGEDBEGCEDCDECBBBE@DD@BA>A?CB@B@@BDBBB=B@=BBBBBBD7BBB +@L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGG ++ +GGGGGGGGDGGGGHHHHHHFHHHHHHHHHHHHHHHHHHHHHHHHGHEHHHHHHHHEHFEFBGGHGGHHBEGEGFHBEHHBHGHHDHHHGHGBHC8>8?ECDEEEEC<BCEEB@91;==BB:B@BB>B@/:::;5==>>A +@L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG ++ +IIIIIIIIIIIIIIIIIIIHIIIIHHIIIIIIIIIHIIIIFGHHHIGIGHHIIIIIIHIIHHIIGFFIHHHGICHGHGHGGHEDHHGDFGGGGGGDB@BCCBDABE@E@BBBBEEBF@?A>?==B@/=?@?>;@BB@ +@L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG ++ +IIIIIIIIIIIIIGHIIIIDIIIIIGIIIFIIIGIIIIIIIICGGIIFBDGG@IFFIFHGHIHDBECEHGHGFFD8FCGCGGGGFGEBDEEEBEEGEGBB>BB@BBCCA=;??=<?;4>A@=?:>@??:@@(@ +@L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGC ++ +IIIIIIIIIIIIIIIIIIIDIIIIIHGIIIIIIIIGIIIFIHIIHIIIGIHFIIHIIHIGHGGHEH<HGHGEFBC>EBEEEFCGEGDBEECEBBCC<@BDBBEDBDBD@BCBCDDDADB@==@?@B=@@B3B=@@ +@L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGG ++ +HHHHHHHHHHDHFHHHHHHHHHHHHGHHHGHHHHHHHHHHHHHHHHHHGGGG>HHHHHEDGDDGFGGGDGGBGEEGBGG8G@DEGGEFEFE<GGEEFEGGHG3DGBBBDDGD@GFDEEDBDBB>BBBDBBD@>?:;??=?B +@L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAG ++ +HHHGGGGGGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHDHHHHHHHHGG1D<?A;=;GFGBGG<GDGDDGGBAADG@HHBHFGHEGE<?=88DDDAD@@BB2:;?@?B>>D;A9A8C +@L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ++ +HHHH@HHHHHHHHHHHHHHGHHHHHHHHHHHHHHHHHHHHHHHHGHHHGHHHHHHHHHHHHGEHHHFHEGEDGDDDGGFFFDDABEEEDDCFGEEDBCAEBEEEEE@EBBEBBEEEE8B@@:<8==<=BAA>A@:@8@ +@L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGG ++ +IIIIIIIIIIIIIIHIIIIIIIIIIHIIIIIIIIIIIHIHFIIHIIHIHHHIIIHHIHHIIIHIGIHIFHIFHEDBIDFFEHHGGHHEFEBIEFFCEED>EDEBEEDBEEHDBEFEBDGEEDDBFEDEBCBBB;CBBB +@L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAG ++ +IIIIIIIIIIIIIHIIIIIIIIIIIHIIIFIIIHIHIIHHIHHIIIIIHFIIGIFDAGIIHFHHEH@IHIIGIHGGIEHGHEHGHFHGEEEIHHEEE@EEEEIEFHBEDFGDFGGEEDEEBB18A@@@B@88@ +@L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIHIGIIGIIIIGIIIGIIIHIIIIIHHHIIIHIIIHIHHI<HFIFHEIHEHGGHCBHBG<DDDA?@AD@8=BAA?<6?AA??1<<9;2546<?+:@ +@L1S76_61 HWI-EAS440_0386:1:25:3312:3259#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGC ++ +IIIIIII:DGGGGFGIIIIHIIIIHFIIIDIIIIIHIIIHDIIICIIIEHIHIIIHIIIHIIHFEE>IIHHGIGE>FEEEFHEEGGGGCHEHGGEGDEEGG@GDDEDGEBEGDBE<EBEBBBC@@@ +@L1S57_62 HWI-EAS440_0386:1:25:3550:3291#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG ++ +IIIIIIIIIHIIDIIIIIIEIIIIHFHIIIHIIIIHFIIIHIIIIIIIEHGIDIIDIGIFFBIIHEDIEEEFGEHEIBFCFDEGHGGGHGHGE>EEDEBEBBDDCCDDC>DCC@C?CBCE@BBBB>9@<>?;@+;<? +@L1S257_63 HWI-EAS440_0386:1:25:5478:3298#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG ++ +IIIIIIIIIIIIIIIIIIIIIIIIHHIIIHIIIIIHIIIIIHHHHIIIHHIIGIHIIIHHIIIHCIHIFHIFFEE@HDHGHFGGGGGEGEEHEGHECGEEGGHBBDDBB8B@B@@A +@L1S281_64 HWI-EAS440_0386:1:25:15346:3296#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCTGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGT ++ +IIIIIIIIIIIIHIIIIIIIII2GGDGGIHIIIIHIIIIHIHIIEIIIIFIIIIIHIIIIIIGIFFDIIIIHIEEDIEIEFHHBEICEBDCEBECDEEEHEBFBCEBDB=BBD?AA? +@L1S76_65 HWI-EAS440_0386:1:25:3468:3322#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGCTGTGCAAGACAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGTGACTGCACGGCTAGAGTTTGTCAGAGGAGG ++ +IIIIHIIIGGGGGIHIIIIHBIIIHIIIIIIIIIGIIHHHHIIIGIIDIHIIIIHHIFEIEEEFDCFFGEGDHGGEGGGGHGEEDGGEEDDGF8EBBCDEEDBBE@@@BCBCBB@EBBBB@==4@??3===99@1B@ +@L1S281_66 HWI-EAS440_0386:1:25:4199:3321#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCATCACAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTTGAAACTGTGGAGCTGGAGTGCAGGAGAGGCAAGTGG ++ +IIIIIIIIIGIIIGIIIIIIIIIIHDIIIIIIIIIHIIGGEGGIHIIIIIHIEIIIEIIIHGIIHIIGIGIEIHHIHHHGEGEC<EHCGGHEFE<EB@BBE>CCEB>B;/=?=BCBCB>BB>@@B<@?<85*928578=@B +@L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ++ +GGGHGHHHHHHHGHHHHHHFHHHHHFHHHHHHHHHHHHHHHHHHEHHHEEGDGHHHHHHHHHHHGHHHHHHHHDGGHGHHHHFEHGGBHBEHEGHGHGGHGDHAEHEHBBDBBBBC>BEEEBBAEAAEEBEAB><@B +@L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG ++ +HHHHHHHHEHHHHHHHHHHGHHHHHHHHHDHHHHHHHHHHHHHHHHHHHHDHHHHEFHHHHH@HHHHHEHHEGEEEHDHEFHEGHE@EHGGHGC@B8DEEECEBEED8;?=??DBCDBCCCBDBB<BBBCE8E3=3=? +@L1S140_69 HWI-EAS440_0386:1:25:4994:3354#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ++ +GGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGFHHHHGGHFHHHHHHHHHHHHGHGHEHHEHD>CECGGHH@GGCFGGEGHGB@BBBEEEEH@BBCCB@B>CE<C@BBEBBADEAA>=A@@=:==? +@L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGG ++ +EEEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDGGGGGGGGGGFGFGGGGDGFGGF>EGEGEFED8DGDEEEBGDGBDFDBDBDEE<BED@@A@8B;=9>8>+>;;49;? +@L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTT ++ +IIIIIHIDIIIIIDIIIIIIIIIIGDIIIEIIIIIIIIFFFIIIHIIIHEIIIIGFIIIGIIHIEHEIFDIFIGGGHEIEDHCGHGHHDEEIEEEEEEGGG>GDEDBB>ADAD@A;>4<=<967:<<=:? +@L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGGAC ++ +HHHHHHHHHHHHHHDHHHHHHHHHHHGHHHHHHHHEHHGGBGGGHHHHHHHHHHGHHHHHHGEGFFGFFGFDFEFHFDHEEHBC2BEC8=??ABBB@D@@>BBBB@5@77==1CC@BB +@L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAG ++ +GGGIIIIIIIIIIIHIIIIGIIIIIDIIIGIIIIIIIHIIIGIIGIIHEGGGCIIFIIIHIIIIHGHIHHIEHHEDDHFBIDFFHHFEGGGHG>EEBEBCEBC@BBBB=69=,:186:EBEBC;;@:@ +@L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA ++ +IIIIIIIIIIIIGIIIIHIIIIIIHHIIIIIIIIIHIIHHIIIIHIIIHGHHIIIHHIHIIIHIEEEIHIIGIFCDIBHHI@HHGEDD>B@DBEBBBEEEBEFCEGEEB<DBBEEBEB@BB<C=B7@B@CB@ +@L1S105_75 HWI-EAS440_0386:1:25:9796:3444#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAAGTGAAAATCCGGGGCTCAACCCCGGAACTGCTTTTGAAACTGTGAGGCTAGAGTGCAGGAGGGGTGAGTGGAATTCCTAGTG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIDIIIIIIIHIHIIGIIGIIHIIIIIIHHIBIIIIIIIGIEIHGIIIEHII@IGIHFDHIGDIHHFEEIGBB<DDFFFFEFDEBBDGGBFCEECDDD@D@FBBDD;BBAA@D9D::?3=<65855;:< +@L1S8_76 HWI-EAS440_0386:1:25:2235:3462#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAGTCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCA ++ +HHHGEGGGFGBGGHHHHHHFCHHHHHHHHHHHHHHDHEFFFG@EEEF<HFHHHHDHF8DHBHHHAGHGFFDADDDFCBEEDDACEBEBEB7>E>>)>@EDCC@B<>:5:6:>68>0A;9>;:@?<>??@B +@L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAG ++ +GGGHHHHHHHGHHHHHHHHGHHHHHGHHHHHHHHHHHHHHHHGHHHHHHHHHHHGBHHHHHBHHHHHHHFHGHBHHFGEGEEBEHHHDDDB@GB=?=?EEE>EBD3=@AABDDD>BBCA<A=A +@L1S281_78 HWI-EAS440_0386:1:25:13919:3507#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGG ++ +IIIIIHIIFIIIGGHIIHIDIHIIGBIIIEIIIHHEIIHEGHHHEHGIDBEGBIFEFFEEHEBHEFCHGGHEHE@BHBFDFEEGEDDEBBBHE@DBBDBEBBEDEEDBE>AECDCDB@BB@:C@BB@B=0=0>?=>@ +@L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAAGTGAAATGCGGGGGCTCAACCCCTGAACTGCTTTGGAAACTGTCATGCTAGAGTGCAGGAGGGGTGAGCGGAATTCCTAGTG ++ +IIIIIIIHIIIIHIIIIIIIIIIIIIIIIIIIIHIIIIIIHIFIIIIIHHIFIIIHGHHIHIIIHHIIFFICIB8BDGD@DBACFEDGCEEBFFFCBBBEBEBAA;8?>?AAABBCC@BB>>C@DB@B@7@@@83;8@89<7+=8=(=5?9? +@L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTAATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTG ++ +<@@GGGGGGGBGGGGGGGGGGGGGGGGGGGGDEGGGGGGGGGGGBGGGGGGDGGGB<BGGGGGGGGGG><GGGDGDGGGEGDGGGGGEGFE<DGDDDDGGGBGDDGD:BBDDD??:<?GGDBB;B +@L1S76_81 HWI-EAS440_0386:1:25:11581:3662#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGG ++ +IIIGIIIIGHIIGGIIIIIHIIIIHIHIIIHIIIIEIEIFFGGGIFIHIIHFIHIHDFIIIGEHEHHIHIGGFEGG<HEGBD>@>ADAEEE@D?A=<=@B@@@B@B>BBBBCA@@)>@<4>1>@@>;>A4:@A +@L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAGTTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG ++ +IIIIIIIIIIIIIIIIIIIHIIIIIDIIIIIIIIIHIIHIIIIIHIIIHDIFIIIIIIIIIIHIGIGIHHIEIFFEIEIHIFIGGHGHDHHHGHGB<EEDHEFGGHBGEDHHHBCBEBEED@B8@@@A@B@@@8?3? +@L1S208_83 HWI-EAS440_0386:1:25:8197:3691#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGAGTGTCG ++ +IIIIIIIIIIIIHIIIIIIIIIIIHGIIIHIIIIIIIIIHHIIIIIGFIIIIIIIIIIIIIHGIFFGIFIIFIGHBIHGIHIGHGGEDDBDBFD>B@@BBB=BBDB?@B:BBBEEECB??A4@4?==? +@L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAG ++ +=B@GDGGGEGEGGGGGGGGEGGGGGDGGGGEEEGGGBGGGGGGGDGGGGGGG@EEBBE???DBGGGEGBGGDGGGGBC?0?<BFGF>BDGDDGCBDDDDDDDDEGGFDDDAG+(2:39DBAAA +@L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCGGTGAAATTTTGGTGCTTAACACCAAACGTGCCGTTGATACTGCTGGGCTAGAGAGTAGTTGCGG ++ +IIIIIIIIHIIIIIIIIIIGIIIIFFIIIFIIIIIHIIIHEIIIHIIIHHIFIIIIHIIIHHHIGHHFHIHEIDBBFGHFHFEHEIIEBDBEEDCDEF=DBDDCBD=D@BDEBDFCC>B@B4B=@=>@=BB>8?
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_libraries_fastq_seqs.fna Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,172 @@ +>L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGATGTGAAAGACATGGGCTCAACCTGTGGACTGCATTGGAAACTGTCATA +>L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTG +>L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTA +>L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAG +>L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG +>L1S257_5 HWI-EAS440_0386:1:25:2639:1894#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG +>L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGT +>L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG +>L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCGACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAG +>L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTT +>L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAG +>L1S76_11 HWI-EAS440_0386:1:25:6540:2228#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTA +>L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTT +>L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG +>L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAG +>L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAG +>L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAA +>L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGG +>L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG +>L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGG +>L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG +>L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG +>L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCG +>L1S76_25 HWI-EAS440_0386:1:25:8159:2639#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTA +>L1S140_26 HWI-EAS440_0386:1:25:11175:2690#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAGGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGC +>L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG +>L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCG +>L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA +>L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG +>L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTA +>L1S140_32 HWI-EAS440_0386:1:25:14127:2819#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTT +>L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTGGTGAAAGATCGAAGCTTAACTTCGGTAAGCCATGGAAACCGTAGGGCT +>L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAG +>L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCGACCGTAAAATTGCAGTTGATACTGGCTG +>L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAG +>L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTC +>L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATAACTTGAGTGCAG +>L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTG +>L1S76_40 HWI-EAS440_0386:1:25:6132:2959#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCAGGGCTCAACCCCGGGACTGCTTTTGAAACTGCAGGGCTAGAGTGCAGGAGGGGCAAGTGG +>L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGG +>L1S57_42 HWI-EAS440_0386:1:25:1831:2983#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGC +>L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGCTGAAACTGGCAGTCTTGAGTACAG +>L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGGTGCAAGCGATAATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGGGTGAAATCCCACAGCCCAACTGTGGAACTGCCTTTGATACTGCCAGGCTTGAGTACCG +>L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG +>L1S208_46 HWI-EAS440_0386:1:25:5972:3048#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGA +>L1S140_47 HWI-EAS440_0386:1:25:9861:3050#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCT +>L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG +>L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTG +>L1S105_50 HWI-EAS440_0386:1:25:9110:3081#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG +>L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGG +>L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG +>L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG +>L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGC +>L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGG +>L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAG +>L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG +>L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGG +>L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAG +>L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGG +>L1S76_61 HWI-EAS440_0386:1:25:3312:3259#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGC +>L1S57_62 HWI-EAS440_0386:1:25:3550:3291#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG +>L1S257_63 HWI-EAS440_0386:1:25:5478:3298#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG +>L1S281_64 HWI-EAS440_0386:1:25:15346:3296#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCTGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGT +>L1S76_65 HWI-EAS440_0386:1:25:3468:3322#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGCTGTGCAAGACAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGTGACTGCACGGCTAGAGTTTGTCAGAGGAGG +>L1S281_66 HWI-EAS440_0386:1:25:4199:3321#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCATCACAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTTGAAACTGTGGAGCTGGAGTGCAGGAGAGGCAAGTGG +>L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG +>L1S140_69 HWI-EAS440_0386:1:25:4994:3354#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGG +>L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTT +>L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGGAC +>L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAG +>L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA +>L1S105_75 HWI-EAS440_0386:1:25:9796:3444#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAAGTGAAAATCCGGGGCTCAACCCCGGAACTGCTTTTGAAACTGTGAGGCTAGAGTGCAGGAGGGGTGAGTGGAATTCCTAGTG +>L1S8_76 HWI-EAS440_0386:1:25:2235:3462#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAGTCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCA +>L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAG +>L1S281_78 HWI-EAS440_0386:1:25:13919:3507#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGG +>L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAAGTGAAATGCGGGGGCTCAACCCCTGAACTGCTTTGGAAACTGTCATGCTAGAGTGCAGGAGGGGTGAGCGGAATTCCTAGTG +>L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTAATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTG +>L1S76_81 HWI-EAS440_0386:1:25:11581:3662#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGG +>L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAGTTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG +>L1S208_83 HWI-EAS440_0386:1:25:8197:3691#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGAGTGTCG +>L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAG +>L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCGGTGAAATTTTGGTGCTTAACACCAAACGTGCCGTTGATACTGCTGGGCTAGAGAGTAGTTGCGG
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_libraries_fastq_seqs.qual Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,584 @@ +>L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[32 34 34 34 38 38 38 38 31 38 30 38 38 38 38 38 38 38 38 35 38 38 38 38 38 + 38 38 38 38 38 38 38 38 38 38 38 35 38 38 33 38 38 38 33 38 38 38 38 38 38 + 38 35 38 36 38 38 35 35 38 31 38 38 38 38 36 36 36 38 38 38 38 38 38 18 23 + 23 38 35 29 35 35 38 38 36 36 31 35 35 35 35 33 29 37 35 38 35 29 31 12 25 + 22 19 28 26 32 9 22 19 28 28 28 28 30 33 33 35 31] +>L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 37 + 39 33 39 39 36 34 34 39 33 35 39 37 36 39 32 36 31 31 33 35 33 28 33 31 31 + 32 17 35 7 20 20 20 18 35 32 32 32 32 36 31 36 36 32 29 26 8 24 26 28 28 + 32 29 22 33 31 28 31 22 23 26 25 23 17 30 34 27 29 27 24 30 27 29 29 29 24 + 23 28 31] +>L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 38 40 40 40 40 38 40 40 40 40 40 38 40 40 39 40 35 40 39 40 39 39 + 37 31 40 40 37 40 40 40 40 40 38 40 40 40 39 40 39 38 39 33 40 40 40 38 39 + 39 38 40 37 36 36 36 37 39 39 39 35 36 36 35 38 29 35 33 36 37 33 35 33 31 + 33 33 28 36 36 33 33 33 36 38 36 35 35 33 32 29 35 31 31 31 31 33 34 33 33 + 26 33 35 31 31 29 25 31 31 29 33 31 30 28 16 31 31 31 18 27 24 22 21 22 28 + 21 29 26 26 30] +>L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 38 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 40 40 40 + 37 40 40 38 36 38 38 35 40 40 40 40 40 37 37 39 40 40 38 35 38 36 38 35 29 + 38 35 38 40 40 36 40 38 40 40 37 39 29 38 33 35 35 38 37 38 38 36 38 33 33 + 17 35 33 35 35 38 33 35 36 33 36 33 33 23 30 27 33 29 33 29 32 31 35 28 18 + 23 28 32 34 29 30 29 31 31 30 35 35 31 29 28 30 28 19 22 23 26 10 26 33 36 + 23 29 33] +>L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 39 40 40 40 40 40 40 39 36 36 40 40 36 40 40 40 40 40 40 38 + 38 39 40 39 36 40 40 39 35 37 34 40 38 39 39 39 34 36 35 33 40 36 40 36 36 + 39 33 33 36 33 33 36 40 39 38 38 38 38 38 35 39 38 38 36 36 38 35 38 30 27 + 17 32 20 35 33 33 33 33 36 33 36 33 33 33 31 33 36 35 36 33 33 33 36 26 31 + 31 31 33 31 31 23 25 31 33 31 31 31 31 32 36 31 31 32 15 31 29 28 27 24 25 + 26 24 28 22 29 25 26 28 28 27 30] +>L1S257_5 HWI-EAS440_0386:1:25:2639:1894#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[39 39 39 38 38 36 38 36 38 38 38 38 38 39 39 39 39 39 39 39 38 39 39 38 38 + 34 38 38 39 37 39 39 39 39 39 39 39 39 36 35 33 38 38 39 37 39 39 39 39 37 + 39 39 39 39 39 37 39 39 39 39 39 36 33 37 34 36 36 35 37 37 37 35 36 28 26 + 31 34 28 34 34 34 32 32 33 33 35 31 31 25 28 26 30 28 33 33 31 29 35 33 35 + 28 31 35 33 33 33 28 35 21 6 28 26 26 32 28 30 28 24 24 28 24 13 24 13 17 + 11 14 17 28 28 28 22 24 31 31 21 31] +>L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 40 40 40 38 40 40 40 35 38 40 40 40 37 40 40 39 40 40 40 40 + 40 40 40 40 40 40 40 40 40 40 36 40 37 38 37 38 38 38 39 40 40 38 40 40 40 + 39 33 39 40 39 38 40 38 40 39 40 31 36 38 31 36 38 37 37 40 37 36 35 37 33 + 34 27 37 36 34 31 33 10 31 33 33 33 33 23 17 28 28 30 33 33 31 27 33 33 31 + 33 33 8 32 32 31 23 28 31 30 31 31 20 30 27 29 28 26 26 17 30 12 30 28 32 + 30] +>L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 33 40 40 40 38 40 40 40 40 40 40 40 40 40 40 37 40 40 40 40 40 40 40 40 40 + 40 40 39 38 40 40 40 40 40 40 40 36 35 38 37 38 36 35 35 38 35 33 38 25 31 + 31 36 34 38 36 37 36 33 39 33 39 37 39 33 33 31 31 38 38 33 33 33 35 33 36 + 34 33 33 38 36 34 33 36 34 33 37 36 33 35 36 33 36 33 33 31 31 17 33 19 30 + 27 28 30 26 32 30 30 32 31 32 10 29 30] +>L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 39 38 38 40 40 39 40 40 40 40 40 40 40 + 39 40 40 40 39 40 40 40 40 40 38 40 39 39 40 40 40 40 39 35 40 40 40 39 39 + 39 35 40 40 40 39 40 40 39 35 39 38 36 39 36 39 38 35 38 39 36 35 38 35 39 + 36 36 37 36 39 38 11 36 35 34 35 34 37 31 36 33 33 36 34 33 31 31 33 21 29 + 31 32 31 36 34 33 33 34 33 33 36 36 32 29 33 33 33 31 31 35 33 32 33 28 31 + 23 31 31] +>L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 38 39 39 39 39 38 39 39 39 39 38 33 39 39 39 39 39 + 39 39 39 39 37 39 39 39 39 36 39 39 39 39 36 37 36 39 37 38 38 37 38 32 32 + 29 34 28 38 38 35 33 35 36 35 36 36 33 34 33 33 36 36 33 33 29 33 35 31 33 + 33 31 35 33 33 33 33 28 34 29 33 34 34 33 29 36 33 33 23 30] +>L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 38 33 38 38 38 36 38 38 38 38 31 38 38 35 38 39 39 + 37 38 39 39 39 39 39 39 38 38 37 36 38 33 38 38 38 18 34 36 29 36 32 32 33 + 38 35 33 37 37 36 35 37 36 29 36 29 35 34 33 31 36 37 37 33 34 29 36 35 33 + 35 35 10 34 32 32 29 29 24 30 28 24 24 35 34 34 30 32 34 32 33 36 33 33 36 + 33 27 33 33 35 31 33 31 31 31 33] +>L1S76_11 HWI-EAS440_0386:1:25:6540:2228#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 + 40 40 40 40 40 40 40 40 40 40 39 40 40 38 40 40 38 40 40 40 40 39 40 40 40 + 40 35 38 40 33 37 39 35 39 33 36 39 39 40 36 35 39 38 39 39 40 37 37 38 38 + 39 31 29 36 36 34 36 33 38 35 36 34 35 36 36 36 36 36 36 35 23 33 29 31 31 + 31 30 30 36 36 33 9 33 22 16 26 17 29 22 31 30 28 28 28 31] +>L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 39 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 + 33 40 40 40 37 40 40 40 40 40 39 40 40 39 39 39 40 40 37 37 40 40 40 37 36 + 40 37 40 40 40 39 40 40 40 40 40 39 38 40 36 36 34 39 36 37 40 35 40 33 34 + 29 38 33 37 37 37 36 36 39 33 36 38 36 33 33 36 37 35 33 34 33 33 37 23 32 + 30 30 32 33 33 35 28 33 31 31 35 33 33 30 30 32 26 26 29 30] +>L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 38 40 + 37 40 40 40 40 40 40 40 40 40 39 40 40 40 39 39 40 39 39 37 40 40 40 38 40 + 40 40 36 38 40 37 39 40 40 38 39 40 40 40 38 39 38 40 38 40 38 39 40 36 35 + 33 37 32 36 39 39 37 36 38 38 35 38 36 27 34 33 37 34 38 35 36 38 38 38 38 + 36 38 39 36 36 34 36 29 34 33 34 31 33 35 33 31] +>L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 35 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 38 37 38 38 38 37 38 + 38 36 38 38 38 38 38 38 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 36 36 + 36 38 34 39 39 39 38 38 38 39 39 39 33 39 38 39 29 36 36 35 33 35 36 39 39 + 39 35 37 33 37 38 29 27 30 30 34 32 34 23 35 35 32 33 35 36 36 33 36 35 33 + 31 33 36 33 31 33 29 33] +>L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 40 40 39 40 + 36 40 40 40 39 40 40 40 38 40 40 40 40 40 40 39 40 39 37 40 40 40 40 40 35 + 40 39 39 40 38 38 40 40 40 40 39 33 39 40 37 40 37 40 40 40 39 39 40 36 36 + 31 38 33 38 35 35 35 35 31 36 39 36 36 36 36 34 33 36 36 36 35 34 37 33 34 + 33 33 37 36 36 40 29 31 33 31 35 33 35 33 36 36 33 24 30 30 30 11 30 27 32 + 29 31 32 28 32 28 27 28 26 9 26 30] +>L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 35 40 40 40 40 40 + 39 40 40 40 39 40 40 40 40 40 39 40 40 39 39 40 40 40 40 39 40 40 40 39 39 + 37 39 38 40 39 39 40 38 38 40 36 39 35 40 34 35 36 40 36 40 40 36 40 33 33 + 33 35 31 37 33 36 36 33 36 36 36 37 35 35 33 36 37 36 33 33 33 33 36 33 33 + 33 33 35 30 33 33 23 23 27 25 27 24 30 33 31 33 31 31 31 32 30 18 31 31 31 + 24 30 31] +>L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[39 39 39 39 39 39 39 39 35 32 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 31 39 39 39 39 38 39 39 + 37 38 39 39 39 39 39 39 39 37 39 39 39 39 38 39 39 39 35 39 39 38 39 38 38 + 39 27 38 36 31 35 36 33 36 37 36 37 39 38 38 38 33 38 31 33 33 36 35 33 35 + 33 31 35 36 33 37 36 36 38 36 36 29 38 33 34 29 35 36 34 36 35 33 29 21 29 + 29 29 32 21 29 29 26 19 29 28 21 25 28 24 28 33 25 31] +>L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 36 39 39 39 39 39 39 39 37 39 39 39 36 39 36 39 39 39 39 39 39 39 33 + 39 39 36 39 38 39 39 39 37 38 38 38 38 33 36 36 39 36 38 34 33 38 35 34 38 + 36 38 38 36 38 38 36 38 36 34 34 36 34 31 36 36 29 33 31 32 28 28 31 22 11 + 23 28 29 26 16 28 24 32 29 29 29 26 32] +>L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 38 40 39 39 40 40 40 40 40 38 40 40 40 40 40 + 37 40 40 40 39 40 40 40 40 40 39 40 40 40 39 40 40 38 40 38 40 40 40 39 40 + 40 37 40 40 40 38 40 40 40 35 39 38 35 40 37 40 37 36 39 40 37 33 39 33 35 + 37 37 37 36 40 36 23 33 35 36 35 35 36 36 37 35 35 39 36 34 34 31 36 32 33 + 34 36 36 33 32 35 18 35 31 33 34 34 34 36 37 29 34 33 33 33 34 23 34 30 33 + 31 28 31 31 27 27 29 33 33 28 31 31 33] +>L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[38 38 38 39 39 39 39 39 38 36 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 38 39 39 39 39 39 33 39 39 39 39 39 39 38 34 38 33 38 36 36 + 38 36 36 38 38 35 33 33 32 35 33 38 35 37 35 36 36 38 34 38 37 36 37 37 34 + 35 33 33 35 33 36 34 34 36 37 39 36 36 36 36 25 39 33 26 32 27 25 25 28 30 + 29 28 26 36 36 36 32 36 31 29 32 34 29 11 25 13 20 17 30 26 25 20 17 20 20 + 25 11 22 31 18 25 19 25 31] +>L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 38 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 38 40 38 40 40 39 + 40 40 40 37 40 39 40 40 40 40 39 39 40 40 40 40 40 36 40 36 40 39 40 35 35 + 38 38 31 38 38 33 38 38 38 39 40 40 40 40 38 40 37 40 38 40 40 39 39 36 36 + 36 38 33 39 36 39 39 37 37 38 39 38 38 38 38 36 38 35 36 33 36 35 36 32 34 + 35 35 34 32 33 33 35 35 33 31 31 31 33 36 33 36 33 34 34 33 35 33 33 33 33 + 35 36 36 30 33 31 29 33] +>L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[38 38 38 39 39 39 39 39 33 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 38 39 39 39 39 39 38 39 39 39 39 39 33 38 39 39 39 39 39 38 + 39 39 33 38 38 36 38 36 35 38 33 38 38 39 39 39 29 39 35 33 38 35 38 39 39 + 38 39 36 35 29 38 33 31 38 36 38 36 38 39 37 33 39 36 35 31 33 35 35 35 39 + 37 35 31 34 29 37 34 36 37 36 39 33 33 29 31 30 25 29 30 30 23 23 17 21 28 + 23 14 25 26 27 27 25 30 29 8 29 32] +>L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 33 39 39 40 40 39 40 39 40 40 40 40 40 + 34 40 40 40 37 40 40 40 40 40 39 40 40 40 39 38 39 39 37 36 40 39 40 36 37 + 40 40 33 40 39 37 39 40 40 39 40 40 38 39 36 39 38 39 38 39 39 38 39 35 33 + 33 40 34 39 36 37 39 36 38 38 35 38 36 36 34 38 39 36 38 35 36 29 38 38 36 + 35 36 38 35 35 35 33 36 36 33 36 36 31 33 33 36 33 33 35 38 35 34 28 32 33 + 33 33 33 30 28 28 12 25 28 28 30] +>L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 40 35 40 40 40 18 40 40 38 38 38 38 38 40 40 40 40 40 31 39 40 40 38 40 40 + 39 38 40 40 40 40 40 39 40 40 39 40 40 40 39 40 40 35 36 38 38 36 38 38 39 + 39 40 39 40 39 40 40 38 35 37 40 33 37 27 35 33 35 35 33 40 35 39 39 40 40 + 38 38 38 37 40 37 36 39 32 32 34 36 32 36 40 35 40 24 40 40 37 34 37 37 36 + 36 36 37 27 33 33 27 35 28 28 30 30 30 27 31] +>L1S76_25 HWI-EAS440_0386:1:25:8159:2639#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 38 40 40 40 40 40 + 38 40 40 40 39 40 40 40 40 40 38 40 39 39 39 39 39 39 40 39 40 39 40 35 36 + 40 38 34 40 37 39 39 40 39 36 40 39 36 39 36 39 36 40 36 40 39 36 40 33 35 + 33 37 33 37 34 35 35 35 38 36 38 36 36 35 35 35 38 34 33 35 33 33 35 32 33 + 36 33 36 35 38 35 36 32 36 33 34 33 34 33 33 36 33 33 31 36 33 22 33 33 31 + 33 33 33 21 30] +>L1S140_26 HWI-EAS440_0386:1:25:11175:2690#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[34 34 34 38 33 38 38 38 36 38 38 38 38 38 38 38 36 38 38 38 38 38 38 38 38 + 35 38 38 38 38 38 36 35 38 38 37 38 38 38 38 38 38 38 38 37 35 35 38 37 38 + 38 36 38 38 38 33 36 38 36 38 35 38 38 38 37 38 29 38 37 38 38 37 38 37 36 + 36 38 36 37 36 37 31 36 36 36 36 37 36 33 36 35 37 35 34 35 31 33 33 36 35 + 36 29 36 35 34 37 33 36 35 33 36 37 29 26 32 29 25 29 32 32 23 29 29 26 32 + 32] +>L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 37 40 39 40 40 40 40 38 40 40 40 40 40 + 37 40 40 40 39 40 40 40 40 40 40 40 40 40 40 35 40 39 40 40 40 39 40 38 40 + 40 39 40 40 40 40 40 40 40 40 37 39 39 40 38 39 38 40 39 40 38 36 35 33 33 + 33 38 33 38 39 37 39 38 34 38 39 38 36 33 36 36 40 36 36 33 36 36 34 36 36 + 39 34 39 33 36 37 33 33 33 31 33 33 33 34 29 36 31 33 30 31 31 30 31 28 31 + 31 33 33 26 29 31 30 29 31 30 29 32] +>L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[35 38 38 36 33 36 36 36 30 33 11 33 30 38 38 38 38 38 38 38 38 38 38 38 38 + 38 38 38 38 38 29 38 38 35 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 38 38 38 38 38 32 29 29 38 31 29 38 27 36 30 33 36 36 31 38 38 35 38 38 35 + 38 33 35 38 36 33 29 35 35 38 35 38 37 37 38 35 23 37 33 33 30 30 29 38 35 + 38 35 33 32 31 32 35 26 32 32 32 36 20 30 33 26 26 19 32 35 35 33 33 33 31 + 33 31 35 16 13 30 23 30 30 12 23 25 25 26 30] +>L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 39 39 39 40 40 40 40 40 40 40 40 39 40 + 33 40 40 40 37 40 40 40 40 40 39 40 40 40 40 40 40 36 37 40 40 37 40 36 39 + 37 39 39 40 40 37 40 40 40 40 39 40 39 40 36 40 39 40 39 40 40 37 40 34 36 + 34 40 33 39 39 40 37 36 39 35 36 38 36 34 33 33 39 36 35 34 33 35 37 34 36 + 36 33 39 33 33 36 34 29 33 28 33 33 33 34 36 33 35 32 31 33 33 29 33 29 33 + 33 31 34 33 28 30 31] +>L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[40 40 40 40 40 40 40 40 39 40 40 40 35 40 40 40 40 40 40 40 40 40 40 40 40 + 37 40 40 40 39 40 40 40 40 40 39 40 40 40 40 39 40 40 40 40 40 40 40 40 40 + 40 40 40 40 36 40 40 40 40 40 40 40 38 40 39 39 36 40 39 40 39 39 40 34 36 + 39 40 39 39 40 40 31 36 39 36 39 37 36 38 35 38 39 38 36 37 36 36 37 38 38 + 33 36 39 33 36 36 27 37 31 31 33 35 35 35 34 33 35 33 35 35 33 33 33 29 33 + 33 33 36 33 36 33 31 31 29 31 28 31 33] +>L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 39 40 39 40 36 38 38 38 36 40 40 40 40 40 40 40 40 40 40 40 40 + 38 40 40 40 39 40 40 40 36 38 33 38 35 35 35 35 38 38 38 40 40 40 33 36 39 + 40 40 37 39 39 40 40 37 40 40 38 35 33 34 31 29 36 34 18 31 30 29 31 38 36 + 38 38 31 31 35 33 35 31 36 23 34 34 28 33 33 35 33 36 32 34 11 18 23 28 28 + 25 26 28 31 30 11 18 28 24 12 27 26 27 30 32 30 30] +>L1S140_32 HWI-EAS440_0386:1:25:14127:2819#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 + 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 38 36 39 39 39 39 39 + 39 39 39 39 39 38 39 39 39 39 39 39 38 36 33 36 36 38 36 38 38 37 38 34 34 + 33 38 31 36 36 37 35 36 36 36 38 35 36 36 33 33 39 36 35 36 33 36 36 38 38 + 33 36 38 33 32 36 33 35 31 32 39 35 34 30 34 36 31 30 33 35 33 25 29 21 23 + 27 28 28 28 33] +>L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[39 39 39 39 39 39 39 39 36 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 37 39 39 39 39 39 39 39 35 33 38 38 37 39 39 39 39 33 38 38 35 35 35 39 39 + 39 39 38 38 39 39 33 37 38 37 34 38 38 37 33 36 38 38 35 38 35 29 35 29 24 + 32 34 32 34 36 36 36 33 36 36 33 33 34 35 33 31 36 37 29 35 33 33 31 34 18 + 9 28 31 32 32 31 28 31 33 32 36 36 33 34 34 33 31 34] +>L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 37 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 39 + 39 40 40 40 39 40 40 39 40 40 39 40 40 40 37 39 40 40 37 39 40 40 40 39 37 + 39 40 40 35 39 39 40 39 40 40 40 40 39 40 36 39 35 40 38 40 39 38 37 35 35 + 34 37 34 33 31 35 36 33 37 36 37 34 37 34 29 36 35 35 33 33 33 33 33 32 32 + 32 30 32 31 33 33 31 36 31 30 29 12 31 32 31 33 30 30 31 35 33 12 32] +>L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 38 39 40 40 40 37 39 + 39 40 39 40 38 40 40 40 40 39 40 40 40 40 38 39 39 40 39 40 38 39 40 36 34 + 34 38 34 37 37 40 37 37 39 35 37 38 33 28 31 31 35 33 33 33 33 33 33 35 33 + 30 31 36 33 33 33 28 33 29 28 33 31 33 33 33 33] +>L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[39 40 40 40 40 40 40 40 40 40 40 40 40 35 40 40 40 39 40 40 40 40 40 40 40 + 33 40 40 40 38 40 40 40 40 40 38 40 38 40 39 40 37 40 39 39 40 40 40 38 39 + 40 40 40 37 39 39 40 38 35 37 40 36 36 40 36 37 40 35 37 39 39 38 36 38 33 + 39 34 37 39 38 36 38 35 38 34 35 36 33 34 34 33 33 36 36 34 29 33 31 33 29 + 33 23 31 33 33 33 25 33 26 30 30 30 24 31 33 29 34 31 29 26 30] +>L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 39 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 + 40 40 40 40 40 40 40 40 40 40 40 40 39 40 39 39 39 40 40 39 40 39 40 36 40 + 40 37 36 39 39 40 40 40 40 40 40 40 39 40 38 40 39 40 33 35 39 36 39 38 39 + 39 39 38 39 39 36 38 35 33 34 35 37 29 35 35 34 33 37 36 31 30 33 33 31 33 + 31 22 23 33 33 26 31 28 31 31 30 31 28 26 32 28 30 30 30 30 31 25 29 28 31 + 28 33] +>L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 38 40 40 40 39 40 40 40 40 40 39 40 40 39 39 39 40 40 40 39 40 40 40 36 36 + 40 37 40 40 40 37 39 40 40 40 39 39 36 40 38 38 38 40 38 40 40 38 40 38 38 + 35 40 36 38 38 39 38 39 35 36 36 32 33 34 34 36 35 33 34 31 33 33 36 33 33 + 35 33 37 36 36 38 32 33 33 31 33 34 34 27 27 30 6 10 28 29 27 27 26 25 32 + 15 30 32] +>L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 38 40 40 40 40 40 40 40 40 38 38 40 40 40 40 40 40 40 40 40 40 + 40 40 40 40 40 40 40 40 35 40 40 40 40 40 40 40 40 39 40 39 39 39 40 40 40 + 39 37 40 40 39 39 40 38 40 40 40 40 39 38 36 40 35 38 37 40 40 39 40 39 37 + 36 40 36 39 38 40 38 39 38 38 36 38 31 36 36 33 38 36 36 33 35 36 36 36 35 + 38 36 39 39 35 38 39 39 38 35 35 34 38 33 35 33 33 30 35 33 35 31 33 30 30 + 32 23 32 30 31 35 27 33 22 12 30] +>L1S76_40 HWI-EAS440_0386:1:25:6132:2959#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[32 29 33 38 38 38 38 38 38 38 35 38 38 38 38 38 38 38 38 31 38 38 38 38 38 + 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 35 38 38 35 38 34 37 + 37 29 30 38 38 38 38 38 38 36 38 38 38 38 38 38 23 38 38 38 38 35 38 38 33 + 38 27 38 36 38 27 38 38 36 35 36 38 38 38 38 36 36 38 16 28 30 30 33 36 37 + 35 29 34 36 29 34 36 34 35 36 33 36 34 30 29 11 10 30 32 28 30 30 28 28 33 + 31 33 33 32 27 29 29 14 28 24 30 23 30 29 29 30] +>L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 37 40 40 40 40 40 38 40 40 40 40 40 + 33 40 40 40 37 40 40 40 38 40 37 40 40 40 39 39 39 39 40 39 40 40 40 37 39 + 40 38 36 40 40 39 39 40 39 39 40 39 29 40 37 40 36 40 39 39 38 39 40 33 34 + 29 38 34 37 36 40 36 36 39 38 36 38 35 36 36 35 38 35 33 34 33 33 33 35 35 + 36 36 39 31 27 33 33 33 36 33 34 33 36 36 37 36 36 33 36 36 36 31 32 25 30 + 31 33 33 27 20 23 6 17 27 27 27 21 24 26 30 32] +>L1S57_42 HWI-EAS440_0386:1:25:1831:2983#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[36 37 37 38 38 38 38 38 38 33 35 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 38 39 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 33 38 + 36 38 38 36 38 31 38 38 38 38 36 37 37 38 36 34 38 38 34 34 36 32 34 35 35 + 35 23 38 26 31 32 31 31 31 33 35 35 33 37 36 39 36 35 33 29 17 31 32 36 36 + 33 29 33 32 32 29 29 24 29 28 32 32 22 22 29 29 31 30] +>L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[33 36 36 38 38 38 38 38 18 36 33 36 35 36 36 36 36 36 38 38 18 27 38 36 36 + 35 27 36 38 38 31 36 35 38 38 33 27 38 36 36 36 36 32 33 13 19 33 33 20 29 + 31 29 11 36 36 27 36 36 38 38 38 38 35 38 37 38 38 38 29 38 38 32 35 33 30 + 30 27 16 21 14 19 20 24 35 37 35 38 37 37 37 35 33 16 20 9 24 25 14 32 32 + 32 32 32 7 30 30 26 26 37 34 34 33 35 35 35 31 29 33 33 35 33 33 33 23 20 + 29 26 32] +>L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[33 33 33 38 38 38 38 38 35 35 38 38 38 32 34 32 31 11 26 27 20 23 26 38 35 + 38 38 38 38 38 38 37 38 38 33 38 38 36 38 38 38 38 38 38 38 38 36 38 36 38 + 36 38 33 38 36 38 38 38 36 38 23 36 36 37 36 37 36 38 37 33 37 32 35 34 34 + 36 33 35 33 37 33 36 36 36 36 32 36 33 33 33 35 35 27 29 32 23 29 29 32 36 + 36 33 36 31 35 33 33 23 35 33 31 33 15 29 24 8 29 29 31 28 23 31 29 25 18 + 25 23 31] +>L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[27 31 33 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 35 38 38 38 38 38 36 36 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 36 38 38 38 38 38 38 33 36 38 38 38 38 38 36 38 38 38 38 38 38 38 38 33 38 + 17 38 33 38 35 38 38 35 36 38 35 38 37 36 38 37 38 36 38 37 37 35 36 36 38 + 35 36 38 36 37 35 36 38 35 35 33 35 37 34 23 37 34 33 35 35 34 34 35 35 33 + 37 33 34 20 32 32 29 32 29 32 27 32 32] +>L1S208_46 HWI-EAS440_0386:1:25:5972:3048#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 + 40 39 40 40 40 40 31 40 40 40 35 40 40 40 40 40 40 40 36 39 40 39 40 39 40 + 40 39 40 40 40 40 40 37 39 40 40 39 38 40 38 39 36 40 40 40 37 39 40 38 39 + 38 35 39 39 39 34 38 36 34 34 35 36 38 39 35 36 35 38 36 33 33 33 36 35 38 + 35 33 38 34 34 34 30 32 31 28 33 31 31 23 33 31 33] +>L1S140_47 HWI-EAS440_0386:1:25:9861:3050#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 38 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 36 39 34 35 + 31 38 35 39 33 39 39 39 39 36 38 38 36 36 32 34 36 31 36 34 36 34 34 33 35 + 33 27 35 28 35 31 31 32 29 23 32 32 32 32 20 31 23 24 30] +>L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 38 40 40 40 39 39 40 40 40 40 40 40 40 40 40 40 40 + 36 40 40 40 36 40 40 40 40 40 39 40 40 39 39 39 39 39 39 39 39 40 40 38 39 + 36 39 40 40 40 39 40 39 37 39 40 34 38 39 36 37 36 40 36 40 39 36 39 31 28 + 33 35 31 35 36 37 36 34 33 25 33 33 33 34 33 34 37 34 33 33 34 28 34 34 34 + 31 23 36 31 34 33 33 34 33 33 33 33 33 35 23 35 31 26 32 32 31 30 30 33 35 + 35 36 38 35 33 32 32 35 28 29 31 31 31] +>L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[39 39 39 39 39 39 39 39 35 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 37 36 + 39 39 39 39 36 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 38 38 39 38 + 39 38 35 39 39 39 37 38 38 38 39 39 39 39 39 39 37 39 38 39 38 39 38 34 36 + 36 17 36 33 27 32 35 31 36 36 36 33 33 36 38 32 38 36 34 36 33 33 23 16 30 + 26 30 30] +>L1S105_50 HWI-EAS440_0386:1:25:9110:3081#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 39 40 38 + 39 40 40 40 39 40 40 40 40 40 39 40 40 38 39 40 40 40 40 39 40 40 40 39 40 + 40 38 40 40 40 39 40 39 40 40 40 39 39 40 38 40 39 40 39 40 40 38 40 35 34 + 33 40 36 37 39 39 37 39 35 38 39 38 36 35 33 36 38 34 36 35 34 35 36 34 33 + 33 33 36 31 35 35 31 33 32 29 32 30 34 33 31 33 31 31 33 35 33 33 33 28 33 + 31 28 33 33 33 33 33 33 35 22 33 33 33] +>L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[38 38 38 38 38 38 38 38 35 38 38 38 38 39 39 39 39 39 39 37 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 36 39 39 39 + 39 39 39 39 39 36 39 37 36 37 33 38 38 39 38 38 39 39 33 36 38 36 38 37 39 + 33 36 39 39 33 39 38 39 39 35 39 39 39 38 39 38 33 39 34 23 29 23 30 36 34 + 35 36 36 36 36 34 27 33 34 36 36 33 31 24 16 26 28 28 33 33 25 33 31 33 33 + 29 33 31 14 25 25 25 26 20 28 28 29 29 32] +>L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 39 + 39 40 40 40 40 40 40 40 40 40 39 40 40 40 40 37 38 39 39 39 40 38 40 38 39 + 39 40 40 40 40 40 40 39 40 40 39 39 40 40 38 37 37 40 39 39 39 38 40 34 39 + 38 39 38 39 38 38 39 36 35 39 39 38 35 37 38 38 38 38 38 38 35 33 31 33 34 + 34 33 35 32 33 36 31 36 31 33 33 33 33 36 36 33 37 31 30 32 29 30 28 28 33 + 31 14 28 30 31 30 29 26 31 33 33 31] +>L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 38 39 40 40 40 40 35 40 40 40 40 40 + 38 40 40 40 37 40 40 40 38 40 40 40 40 40 40 40 40 34 38 38 40 40 37 33 35 + 38 38 31 40 37 37 40 37 39 38 39 40 39 35 33 36 34 36 39 38 39 38 37 37 35 + 23 37 34 38 34 38 38 38 38 37 38 36 33 35 36 36 36 33 36 36 38 36 38 33 33 + 29 33 33 31 33 33 34 34 32 28 26 30 30 28 27 30 26 19 29 32 31 28 30 25 29 + 31 30 30 25 31 31 7 31] +>L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 35 40 40 40 40 40 + 39 38 40 40 40 40 40 40 40 40 38 40 40 40 37 40 39 40 40 39 40 40 40 38 40 + 39 37 40 40 39 40 40 39 40 38 39 38 38 39 36 39 27 39 38 39 38 36 37 33 34 + 29 36 33 36 36 36 37 34 38 36 38 35 33 36 36 34 36 33 33 34 34 27 31 33 35 + 33 33 36 35 33 35 33 35 31 33 34 33 34 35 35 35 32 35 33 31 28 28 31 30 31 + 33 28 31 31 33 18 33 28 31 31] +>L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[39 39 39 39 39 39 39 39 39 39 35 39 37 39 39 39 39 39 39 39 39 39 39 39 39 + 38 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 38 + 38 38 29 39 39 39 39 39 36 35 38 35 35 38 37 38 38 38 35 38 38 33 38 36 36 + 38 33 38 38 23 38 31 35 36 38 38 36 37 36 37 36 27 38 38 36 36 37 36 38 38 + 39 38 18 35 38 33 33 33 35 35 38 35 31 38 37 35 36 36 35 33 35 33 33 29 33 + 33 33 35 33 33 35 31 29 30 25 26 30 30 28 30 33] +>L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[39 39 39 38 38 38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 39 + 39 39 39 38 38 16 35 27 30 32 26 28 26 38 37 38 33 38 38 27 38 35 38 35 35 + 38 38 33 32 32 35 38 31 39 39 33 39 37 38 39 36 38 36 27 30 28 23 23 35 35 + 35 32 35 31 31 33 33 17 25 26 30 31 30 33 29 29 35 26 32 24 32 23 34] +>L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[39 39 39 39 31 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 38 39 + 39 39 39 39 39 39 39 39 39 39 39 38 36 39 39 39 37 39 36 38 36 35 38 35 35 + 35 38 38 37 37 37 35 35 32 33 36 36 36 35 35 34 37 38 36 36 35 33 34 32 36 + 33 36 36 36 36 36 31 36 33 33 36 33 33 36 36 36 36 23 33 31 31 25 27 23 28 + 28 27 28 33 32 32 29 32 31 25 31 23 31] +>L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 + 39 40 40 40 40 40 40 40 40 40 40 40 39 40 39 37 40 40 39 40 40 39 40 39 39 + 39 40 40 40 39 39 40 39 39 40 40 40 39 40 38 40 39 40 37 39 40 37 39 36 35 + 33 40 35 37 37 36 39 39 38 38 39 39 36 37 36 33 40 36 37 37 34 36 36 35 29 + 36 35 36 33 36 36 35 33 36 36 39 35 33 36 37 36 33 35 38 36 36 35 35 33 37 + 36 35 36 33 34 33 33 33 26 34 33 33 33] +>L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 + 39 40 40 40 37 40 40 40 39 40 39 40 40 39 39 40 39 39 40 40 40 40 40 39 37 + 40 40 38 40 37 35 32 38 40 40 39 37 39 39 36 39 31 40 39 40 40 38 40 39 38 + 38 40 36 39 38 39 36 39 38 39 37 39 38 36 36 36 40 39 39 36 36 36 31 36 36 + 36 36 40 36 37 39 33 36 35 37 38 35 37 38 38 36 36 35 36 36 33 33 16 23 32 + 31 31 31 33 31 23 23 31] +>L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 39 40 40 40 40 40 40 40 40 40 40 40 39 40 38 40 40 38 40 40 40 40 38 40 40 + 40 38 40 40 40 39 40 40 40 40 40 39 39 39 40 40 40 39 40 40 40 39 40 39 39 + 40 27 39 37 40 37 39 36 40 39 36 39 38 38 39 34 33 39 33 38 27 35 35 35 32 + 30 31 32 35 31 23 28 33 32 32 30 27 21 30 32 32 30 30 16 27 27 24 26 17 20 + 19 21 27 30 10 25 31] +>L1S76_61 HWI-EAS440_0386:1:25:3312:3259#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 25 35 38 38 38 38 37 38 40 40 40 40 39 40 40 40 40 39 + 37 40 40 40 35 40 40 40 40 40 39 40 40 40 39 35 40 40 40 34 40 40 40 36 39 + 40 39 40 40 40 39 40 40 40 39 40 40 39 37 36 36 29 40 40 39 39 38 40 38 36 + 29 37 36 36 36 37 39 36 36 38 38 38 38 34 39 36 39 38 38 36 38 35 36 36 38 + 38 31 38 35 35 36 35 38 36 33 36 38 35 33 36 27 36 33 36 33 33 33 34 31 31 + 31] +>L1S57_62 HWI-EAS440_0386:1:25:3550:3291#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 39 40 40 35 40 40 40 40 40 40 36 40 40 40 40 39 + 37 39 40 40 40 39 40 40 40 40 39 37 40 40 40 39 40 40 40 40 40 40 40 36 39 + 38 40 35 40 40 35 40 38 40 37 37 33 40 40 39 36 35 40 36 36 36 37 38 36 39 + 36 40 33 37 34 37 35 36 38 39 38 38 38 39 38 39 38 36 29 36 36 35 36 33 36 + 33 33 35 35 34 34 35 35 34 29 35 34 34 31 34 30 34 33 34 36 31 33 33 33 33 + 29 24 31 27 29 30 26 31 10 26 27 30] +>L1S257_63 HWI-EAS440_0386:1:25:5478:3298#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 + 39 40 40 40 39 40 40 40 40 40 39 40 40 40 40 40 39 39 39 39 40 40 40 39 39 + 40 40 38 40 39 40 40 40 39 39 40 40 40 39 34 40 39 40 37 39 40 37 37 36 36 + 31 39 35 39 38 39 37 38 38 38 38 38 36 38 36 36 39 36 38 39 36 34 38 36 36 + 38 38 39 33 33 35 35 33 33 23 33 31 33 31 31 32] +>L1S281_64 HWI-EAS440_0386:1:25:15346:3296#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 17 38 38 + 35 38 38 40 39 40 40 40 40 39 40 40 40 40 39 40 39 40 40 36 40 40 40 40 37 + 40 40 40 40 40 39 40 40 40 40 40 40 38 40 37 37 35 40 40 40 40 39 40 36 36 + 35 40 36 40 36 37 39 39 33 36 40 34 36 33 35 34 36 33 36 34 35 36 36 36 39 + 36 33 37 33 34 36 33 35 33 28 33 33 35 30 32 32 30] +>L1S76_65 HWI-EAS440_0386:1:25:3468:3322#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 39 40 40 40 38 38 38 38 38 40 39 40 40 40 40 39 33 40 40 40 39 + 40 40 40 40 40 40 40 40 40 38 40 40 39 39 39 39 40 40 40 38 40 40 35 40 39 + 40 40 40 40 39 39 40 37 36 40 36 36 36 37 35 34 37 37 38 36 38 35 39 38 38 + 36 38 38 38 38 39 38 36 36 35 38 38 36 36 35 35 38 37 23 36 33 33 34 35 36 + 36 35 33 33 36 31 31 31 33 34 33 34 33 33 31 36 33 33 33 33 31 28 28 19 31 + 30 30 18 28 28 28 24 24 31 16 33 31] +>L1S281_66 HWI-EAS440_0386:1:25:4199:3321#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 38 40 40 40 38 40 40 40 40 40 40 40 40 40 40 39 + 35 40 40 40 40 40 40 40 40 40 39 40 40 38 38 36 38 38 40 39 40 40 40 40 40 + 39 40 36 40 40 40 36 40 40 40 39 38 40 40 39 40 40 38 40 38 40 36 40 39 39 + 40 39 39 39 38 36 38 36 34 27 36 39 34 38 38 39 36 37 36 27 36 33 31 33 33 + 36 29 34 34 36 33 29 33 26 14 28 30 28 33 34 33 34 33 29 33 33 29 31 31 33 + 27 31 30 27 23 20 9 24 17 23 20 22 23 28 31 33] +>L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 38 39 39 39 39 39 39 39 38 39 39 39 39 39 39 37 39 39 39 39 39 + 37 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 36 39 39 39 36 36 + 38 35 38 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 35 38 + 38 39 38 39 39 39 39 37 36 39 38 38 33 39 33 36 39 36 38 39 38 39 38 38 39 + 38 35 39 32 36 39 36 39 33 33 35 33 33 33 33 34 29 33 36 36 36 33 33 32 36 + 32 32 36 36 33 36 32 33 29 27 31 33] +>L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[39 39 39 39 39 39 39 39 36 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 + 39 39 39 39 35 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 35 39 39 39 39 36 37 39 39 39 39 39 31 39 39 39 39 39 36 39 39 36 38 36 36 + 36 39 35 39 36 37 39 36 38 39 36 31 36 39 38 38 39 38 34 31 33 23 35 36 36 + 36 34 36 33 36 36 35 23 26 30 28 30 30 35 33 34 35 33 34 34 34 33 35 33 33 + 27 33 33 33 34 36 23 36 18 28 18 28 30] +>L1S140_69 HWI-EAS440_0386:1:25:4994:3354#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 37 39 39 39 39 38 38 + 39 37 39 39 39 39 39 39 39 39 39 39 39 39 38 39 38 39 36 39 39 36 39 35 29 + 34 36 34 38 38 39 39 31 38 38 34 37 38 38 36 38 39 38 33 31 33 33 33 36 36 + 36 36 39 31 33 33 34 34 33 31 33 29 34 36 27 34 31 33 33 36 33 33 32 35 36 + 32 32 29 28 32 31 31 28 25 28 28 30] +>L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[36 36 36 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 38 35 38 38 38 38 38 38 38 38 38 38 37 38 37 38 38 38 38 35 38 37 38 38 37 + 29 36 38 36 38 36 37 36 35 23 35 38 35 36 36 36 33 38 35 38 33 35 37 35 33 + 35 33 35 36 36 27 33 36 35 31 31 32 31 23 33 26 28 24 29 23 29 10 29 26 26 + 19 24 26 30] +>L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 39 40 35 40 40 40 40 40 35 40 40 40 40 40 40 40 40 40 40 38 + 35 40 40 40 36 40 40 40 40 40 40 40 40 37 37 37 40 40 40 39 40 40 40 39 36 + 40 40 40 40 38 37 40 40 40 38 40 40 39 40 36 39 36 40 37 35 40 37 40 38 38 + 38 39 36 40 36 35 39 34 38 39 38 39 39 35 36 36 40 36 36 36 36 36 36 38 38 + 38 29 38 35 36 35 33 33 29 32 35 32 35 31 32 26 29 19 27 28 27 24 21 22 25 + 27 27 28 25 30] +>L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[39 39 39 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 39 39 39 39 39 39 + 39 38 39 39 39 39 39 39 39 39 36 39 39 38 38 33 38 38 38 39 39 39 39 39 39 + 39 39 39 39 38 39 39 39 39 39 39 38 36 38 37 37 38 37 37 38 37 35 37 36 37 + 39 37 35 39 36 36 39 33 34 17 33 36 34 23 28 30 30 32 33 33 33 31 35 31 31 + 29 33 33 33 33 31 20 31 22 22 28 28 16 34 34 31 33 33] +>L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[38 38 38 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 38 40 40 40 40 40 + 35 40 40 40 38 40 40 40 40 40 40 40 39 40 40 40 38 40 40 38 40 40 39 36 38 + 38 38 34 40 40 37 40 40 40 39 40 40 40 40 39 38 39 40 39 39 40 36 39 39 36 + 35 35 39 37 33 40 35 37 37 39 39 37 36 38 38 38 39 38 29 36 36 33 36 33 34 + 36 33 34 31 33 33 33 33 28 21 24 28 11 25 16 23 21 25 36 33 36 33 34 26 26 + 31 25 31] +>L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 39 40 40 40 40 40 40 39 + 39 40 40 40 40 40 40 40 40 40 39 40 40 39 39 40 40 40 40 39 40 40 40 39 38 + 39 39 40 40 40 39 39 40 39 40 40 40 39 40 36 36 36 40 39 40 40 38 40 37 34 + 35 40 33 39 39 40 31 39 39 38 36 35 35 29 33 31 35 33 36 33 33 33 36 36 36 + 33 36 37 34 36 38 36 36 33 27 35 33 33 36 36 33 36 33 31 33 33 27 34 28 33 + 22 31 33 31 34 33 31] +>L1S105_75 HWI-EAS440_0386:1:25:9796:3444#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 35 40 40 40 40 40 40 40 39 40 39 40 40 38 40 40 38 40 40 39 40 40 40 40 40 + 40 39 39 40 33 40 40 40 40 40 40 40 38 40 36 40 39 38 40 40 40 36 39 40 40 + 31 40 38 40 39 37 35 39 40 38 35 40 39 39 37 36 36 40 38 33 33 27 35 35 37 + 37 37 37 36 37 35 36 33 33 35 38 38 33 37 34 36 36 34 35 35 35 31 35 31 37 + 33 33 35 35 26 33 33 32 32 31 35 24 35 25 25 30 18 28 27 21 20 23 20 20 26 + 25 27] +>L1S8_76 HWI-EAS440_0386:1:25:2235:3462#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[39 39 39 38 36 38 38 38 37 38 33 38 38 39 39 39 39 39 39 37 34 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 35 39 36 37 37 37 38 31 36 36 36 37 27 39 37 + 39 39 39 39 35 39 37 23 35 39 33 39 39 39 32 38 39 38 37 37 35 32 35 35 35 + 37 34 33 36 36 35 35 32 34 36 33 36 33 36 33 22 29 36 29 29 8 29 31 36 35 + 34 34 31 33 27 29 25 20 25 21 25 29 21 23 29 15 32 26 24 29 26 25 31 30 27 + 29 30 30 31 33] +>L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 38 39 39 39 39 39 + 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 + 39 39 39 39 38 33 39 39 39 39 39 33 39 39 39 39 39 39 39 37 39 38 39 33 39 + 39 37 38 36 38 36 36 33 36 39 39 39 35 35 35 33 31 38 33 28 30 28 30 36 36 + 36 29 36 33 35 18 28 31 32 32 33 35 35 35 29 33 33 34 32 27 32 28 32] +>L1S281_78 HWI-EAS440_0386:1:25:13919:3507#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 39 40 40 37 40 40 40 38 38 39 40 40 39 40 35 40 39 40 40 38 + 33 40 40 40 36 40 40 40 39 39 36 40 40 39 36 38 39 39 39 36 39 38 40 35 33 + 36 38 33 40 37 36 37 37 36 36 39 36 33 39 36 37 34 39 38 38 39 36 39 36 31 + 33 39 33 37 35 37 36 36 38 36 35 35 36 33 33 33 39 36 31 35 33 33 35 33 36 + 33 33 36 35 36 36 35 33 36 29 32 36 34 35 34 35 33 31 33 33 31 25 34 31 33 + 33 31 33 28 15 28 15 29 30 28 29 31] +>L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 39 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 + 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 39 40 37 40 40 40 40 40 39 39 + 40 37 40 40 40 39 38 39 39 40 39 40 40 40 39 39 40 40 37 37 40 34 40 33 23 + 33 35 38 35 31 35 33 32 34 37 36 35 38 34 36 36 33 37 37 37 34 33 33 33 36 + 33 36 33 32 32 26 23 30 29 30 32 32 32 33 33 34 34 31 33 33 29 29 34 31 35 + 33 31 33 31 22 31 31 31 23 18 26 23 31 23 24 27 22 10 28 23 28 7 28 20 30 + 24 30] +>L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[27 31 31 38 38 38 38 38 38 38 33 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 38 38 38 38 38 38 35 36 38 38 38 38 38 38 38 38 38 38 38 33 38 38 38 38 38 + 38 35 38 38 38 33 27 33 38 38 38 38 38 38 38 38 38 38 29 27 38 38 38 35 38 + 35 38 38 38 36 38 35 38 38 38 38 38 36 38 37 36 27 35 38 35 35 35 35 38 38 + 38 33 38 35 35 38 35 25 33 33 35 35 35 30 30 25 27 30 38 38 35 33 33 26 33] +>L1S76_81 HWI-EAS440_0386:1:25:11581:3662#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 38 40 40 40 40 38 39 40 40 38 38 40 40 40 40 40 39 40 40 40 40 39 + 40 39 40 40 40 39 40 40 40 40 36 40 36 40 37 37 38 38 38 40 37 40 39 40 40 + 39 37 40 39 40 39 35 37 40 40 40 38 36 39 36 39 39 40 39 40 38 38 37 36 38 + 38 27 39 36 38 33 35 29 31 29 32 35 32 36 36 36 31 35 30 32 28 27 28 31 33 + 31 31 31 33 31 33 29 33 33 33 33 34 32 31 31 8 29 31 27 19 29 16 29 31 31 + 29 26 29 32 19 25 31 32] +>L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 + 35 40 40 40 40 40 40 40 40 40 39 40 40 39 40 40 40 40 40 39 40 40 40 39 35 + 40 37 40 40 40 40 40 40 40 40 40 40 39 40 38 40 38 40 39 39 40 36 40 37 37 + 36 40 36 40 39 40 37 40 38 38 39 38 39 35 39 39 39 38 39 38 33 27 36 36 35 + 39 36 37 38 38 39 33 38 36 35 39 39 39 33 34 33 36 33 36 36 35 31 33 23 31 + 31 31 32 31 33 31 31 31 23 30 18 30] +>L1S208_83 HWI-EAS440_0386:1:25:8197:3691#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 39 + 38 40 40 40 39 40 40 40 40 40 40 40 40 40 39 39 40 40 40 40 40 38 37 40 40 + 40 40 40 40 40 40 40 40 40 40 40 39 38 40 37 37 38 40 37 40 40 37 40 38 39 + 33 40 39 38 40 39 40 38 39 38 38 36 35 35 33 35 33 37 35 29 33 31 31 33 33 + 33 28 33 33 35 33 30 31 33 25 33 33 33 36 36 36 34 33 30 30 32 19 31 19 30 + 28 28 30] +>L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[28 33 31 38 35 38 38 38 36 38 36 38 38 38 38 38 38 38 38 36 38 38 38 38 38 + 35 38 38 38 38 36 36 36 38 38 38 33 38 38 38 38 38 38 38 35 38 38 38 38 38 + 38 38 31 36 36 33 33 36 30 30 30 35 33 38 38 38 36 38 33 38 38 35 38 38 38 + 38 33 34 30 15 30 27 33 37 38 37 29 33 35 38 35 35 38 34 33 35 35 35 35 35 + 35 35 35 36 38 38 37 35 35 35 32 38 10 7 17 25 18 24 35 33 32 32 32] +>L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 37 + 37 40 40 40 37 40 40 40 40 40 39 40 40 40 39 36 40 40 40 39 40 40 40 39 39 + 40 37 40 40 40 40 39 40 40 40 39 39 39 40 38 39 39 37 39 40 39 36 40 35 33 + 33 37 38 39 37 39 37 36 39 36 40 40 36 33 35 33 36 36 35 34 35 36 37 28 35 + 33 35 35 34 33 35 28 35 31 33 35 36 33 35 37 34 34 29 33 31 33 19 33 28 31 + 28 29 31 28 33 33 29 23 30]
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/summarize_taxa_L2.tabular Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,9 @@ +#SampleID BarcodeSequence LinkerPrimerSequence SampleType Year Month Day Subject ReportedAntibioticUsage DaysSinceExperimentStart Description k__Bacteria;p__Bacteroidetes k__Bacteria;p__Firmicutes +L1S8 AGCTGACTAGTC GTGCCAGCMGCCGCGGTAA gut 2008 10 28 1 Yes 0 1_Fece_10_28_2008 0.625 0.375 +L1S140 ATGGCAGCTCTA GTGCCAGCMGCCGCGGTAA gut 2008 10 28 2 Yes 0 2_Fece_10_28_2008 0.875 0.125 +L1S57 ACACACTATGGC GTGCCAGCMGCCGCGGTAA gut 2009 1 20 1 No 84 1_Fece_1_20_2009 0.714285714286 0.285714285714 +L1S208 CTGAGATACGCG GTGCCAGCMGCCGCGGTAA gut 2009 1 20 2 No 84 2_Fece_1_20_2009 0.777777777778 0.222222222222 +L1S76 ACTACGTGTGGT GTGCCAGCMGCCGCGGTAA gut 2009 2 17 1 No 112 1_Fece_2_17_2009 0.75 0.25 +L1S105 AGTGCGATGCGT GTGCCAGCMGCCGCGGTAA gut 2009 3 17 1 No 140 1_Fece_3_17_2009 0.857142857143 0.142857142857 +L1S257 CCGACTGAGATG GTGCCAGCMGCCGCGGTAA gut 2009 3 17 2 No 140 2_Fece_3_17_2009 1.0 0.0 +L1S281 CCTCTCGTGATC GTGCCAGCMGCCGCGGTAA gut 2009 4 14 2 No 168 2_Fece_4_14_2009 0.875 0.125
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/summarize_taxa_L3.tabular Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,9 @@ +#SampleID BarcodeSequence LinkerPrimerSequence SampleType Year Month Day Subject ReportedAntibioticUsage DaysSinceExperimentStart Description k__Bacteria;p__Bacteroidetes k__Bacteria;p__Firmicutes k__Bacteria;p__Bacteroidetes;c__Bacteroidia k__Bacteria;p__Firmicutes;c__Clostridia +L1S8 AGCTGACTAGTC GTGCCAGCMGCCGCGGTAA gut 2008 10 28 1 Yes 0 1_Fece_10_28_2008 0.625 0.375 0.625 0.375 +L1S140 ATGGCAGCTCTA GTGCCAGCMGCCGCGGTAA gut 2008 10 28 2 Yes 0 2_Fece_10_28_2008 0.875 0.125 0.875 0.125 +L1S57 ACACACTATGGC GTGCCAGCMGCCGCGGTAA gut 2009 1 20 1 No 84 1_Fece_1_20_2009 0.714285714286 0.285714285714 0.714285714286 0.285714285714 +L1S208 CTGAGATACGCG GTGCCAGCMGCCGCGGTAA gut 2009 1 20 2 No 84 2_Fece_1_20_2009 0.777777777778 0.222222222222 0.777777777778 0.222222222222 +L1S76 ACTACGTGTGGT GTGCCAGCMGCCGCGGTAA gut 2009 2 17 1 No 112 1_Fece_2_17_2009 0.75 0.25 0.75 0.25 +L1S105 AGTGCGATGCGT GTGCCAGCMGCCGCGGTAA gut 2009 3 17 1 No 140 1_Fece_3_17_2009 0.857142857143 0.142857142857 0.857142857143 0.142857142857 +L1S257 CCGACTGAGATG GTGCCAGCMGCCGCGGTAA gut 2009 3 17 2 No 140 2_Fece_3_17_2009 1.0 0.0 1.0 0.0 +L1S281 CCTCTCGTGATC GTGCCAGCMGCCGCGGTAA gut 2009 4 14 2 No 168 2_Fece_4_14_2009 0.875 0.125 0.875 0.125
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/summarize_taxa_L4.tabular Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,9 @@ +#SampleID BarcodeSequence LinkerPrimerSequence SampleType Year Month Day Subject ReportedAntibioticUsage DaysSinceExperimentStart Description k__Bacteria;p__Bacteroidetes k__Bacteria;p__Firmicutes k__Bacteria;p__Bacteroidetes;c__Bacteroidia k__Bacteria;p__Firmicutes;c__Clostridia k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales +L1S8 AGCTGACTAGTC GTGCCAGCMGCCGCGGTAA gut 2008 10 28 1 Yes 0 1_Fece_10_28_2008 0.625 0.375 0.625 0.375 0.625 0.375 +L1S140 ATGGCAGCTCTA GTGCCAGCMGCCGCGGTAA gut 2008 10 28 2 Yes 0 2_Fece_10_28_2008 0.875 0.125 0.875 0.125 0.875 0.125 +L1S57 ACACACTATGGC GTGCCAGCMGCCGCGGTAA gut 2009 1 20 1 No 84 1_Fece_1_20_2009 0.714285714286 0.285714285714 0.714285714286 0.285714285714 0.714285714286 0.285714285714 +L1S208 CTGAGATACGCG GTGCCAGCMGCCGCGGTAA gut 2009 1 20 2 No 84 2_Fece_1_20_2009 0.777777777778 0.222222222222 0.777777777778 0.222222222222 0.777777777778 0.222222222222 +L1S76 ACTACGTGTGGT GTGCCAGCMGCCGCGGTAA gut 2009 2 17 1 No 112 1_Fece_2_17_2009 0.75 0.25 0.75 0.25 0.75 0.25 +L1S105 AGTGCGATGCGT GTGCCAGCMGCCGCGGTAA gut 2009 3 17 1 No 140 1_Fece_3_17_2009 0.857142857143 0.142857142857 0.857142857143 0.142857142857 0.857142857143 0.142857142857 +L1S257 CCGACTGAGATG GTGCCAGCMGCCGCGGTAA gut 2009 3 17 2 No 140 2_Fece_3_17_2009 1.0 0.0 1.0 0.0 1.0 0.0 +L1S281 CCTCTCGTGATC GTGCCAGCMGCCGCGGTAA gut 2009 4 14 2 No 168 2_Fece_4_14_2009 0.875 0.125 0.875 0.125 0.875 0.125
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/summarize_taxa_L5.tabular Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,9 @@ +#SampleID BarcodeSequence LinkerPrimerSequence SampleType Year Month Day Subject ReportedAntibioticUsage DaysSinceExperimentStart Description k__Bacteria;p__Bacteroidetes k__Bacteria;p__Firmicutes k__Bacteria;p__Bacteroidetes;c__Bacteroidia k__Bacteria;p__Firmicutes;c__Clostridia k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Rikenellaceae k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__ k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae +L1S8 AGCTGACTAGTC GTGCCAGCMGCCGCGGTAA gut 2008 10 28 1 Yes 0 1_Fece_10_28_2008 0.625 0.375 0.625 0.375 0.625 0.375 0.625 0.0 0.0 0.125 0.25 +L1S140 ATGGCAGCTCTA GTGCCAGCMGCCGCGGTAA gut 2008 10 28 2 Yes 0 2_Fece_10_28_2008 0.875 0.125 0.875 0.125 0.875 0.125 0.875 0.0 0.0 0.125 0.0 +L1S57 ACACACTATGGC GTGCCAGCMGCCGCGGTAA gut 2009 1 20 1 No 84 1_Fece_1_20_2009 0.714285714286 0.285714285714 0.714285714286 0.285714285714 0.714285714286 0.285714285714 0.714285714286 0.0 0.285714285714 0.0 0.0 +L1S208 CTGAGATACGCG GTGCCAGCMGCCGCGGTAA gut 2009 1 20 2 No 84 2_Fece_1_20_2009 0.777777777778 0.222222222222 0.777777777778 0.222222222222 0.777777777778 0.222222222222 0.555555555556 0.222222222222 0.0 0.222222222222 0.0 +L1S76 ACTACGTGTGGT GTGCCAGCMGCCGCGGTAA gut 2009 2 17 1 No 112 1_Fece_2_17_2009 0.75 0.25 0.75 0.25 0.75 0.25 0.75 0.0 0.0 0.25 0.0 +L1S105 AGTGCGATGCGT GTGCCAGCMGCCGCGGTAA gut 2009 3 17 1 No 140 1_Fece_3_17_2009 0.857142857143 0.142857142857 0.857142857143 0.142857142857 0.857142857143 0.142857142857 0.857142857143 0.0 0.0 0.0 0.142857142857 +L1S257 CCGACTGAGATG GTGCCAGCMGCCGCGGTAA gut 2009 3 17 2 No 140 2_Fece_3_17_2009 1.0 0.0 1.0 0.0 1.0 0.0 0.833333333333 0.166666666667 0.0 0.0 0.0 +L1S281 CCTCTCGTGATC GTGCCAGCMGCCGCGGTAA gut 2009 4 14 2 No 168 2_Fece_4_14_2009 0.875 0.125 0.875 0.125 0.875 0.125 0.625 0.25 0.0 0.0 0.125
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/summarize_taxa_L6.tabular Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,9 @@ +#SampleID BarcodeSequence LinkerPrimerSequence SampleType Year Month Day Subject ReportedAntibioticUsage DaysSinceExperimentStart Description k__Bacteria;p__Bacteroidetes k__Bacteria;p__Firmicutes k__Bacteria;p__Bacteroidetes;c__Bacteroidia k__Bacteria;p__Firmicutes;c__Clostridia k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Rikenellaceae k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__ k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Rikenellaceae;g__ k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__ k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;Other k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Roseburia k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__Faecalibacterium +L1S8 AGCTGACTAGTC GTGCCAGCMGCCGCGGTAA gut 2008 10 28 1 Yes 0 1_Fece_10_28_2008 0.625 0.375 0.625 0.375 0.625 0.375 0.625 0.0 0.0 0.125 0.25 0.625 0.0 0.0 0.125 0.0 0.25 +L1S140 ATGGCAGCTCTA GTGCCAGCMGCCGCGGTAA gut 2008 10 28 2 Yes 0 2_Fece_10_28_2008 0.875 0.125 0.875 0.125 0.875 0.125 0.875 0.0 0.0 0.125 0.0 0.875 0.0 0.0 0.125 0.0 0.0 +L1S57 ACACACTATGGC GTGCCAGCMGCCGCGGTAA gut 2009 1 20 1 No 84 1_Fece_1_20_2009 0.714285714286 0.285714285714 0.714285714286 0.285714285714 0.714285714286 0.285714285714 0.714285714286 0.0 0.285714285714 0.0 0.0 0.714285714286 0.0 0.285714285714 0.0 0.0 0.0 +L1S208 CTGAGATACGCG GTGCCAGCMGCCGCGGTAA gut 2009 1 20 2 No 84 2_Fece_1_20_2009 0.777777777778 0.222222222222 0.777777777778 0.222222222222 0.777777777778 0.222222222222 0.555555555556 0.222222222222 0.0 0.222222222222 0.0 0.555555555556 0.222222222222 0.0 0.0 0.222222222222 0.0 +L1S76 ACTACGTGTGGT GTGCCAGCMGCCGCGGTAA gut 2009 2 17 1 No 112 1_Fece_2_17_2009 0.75 0.25 0.75 0.25 0.75 0.25 0.75 0.0 0.0 0.25 0.0 0.75 0.0 0.0 0.25 0.0 0.0 +L1S105 AGTGCGATGCGT GTGCCAGCMGCCGCGGTAA gut 2009 3 17 1 No 140 1_Fece_3_17_2009 0.857142857143 0.142857142857 0.857142857143 0.142857142857 0.857142857143 0.142857142857 0.857142857143 0.0 0.0 0.0 0.142857142857 0.857142857143 0.0 0.0 0.0 0.0 0.142857142857 +L1S257 CCGACTGAGATG GTGCCAGCMGCCGCGGTAA gut 2009 3 17 2 No 140 2_Fece_3_17_2009 1.0 0.0 1.0 0.0 1.0 0.0 0.833333333333 0.166666666667 0.0 0.0 0.0 0.833333333333 0.166666666667 0.0 0.0 0.0 0.0 +L1S281 CCTCTCGTGATC GTGCCAGCMGCCGCGGTAA gut 2009 4 14 2 No 168 2_Fece_4_14_2009 0.875 0.125 0.875 0.125 0.875 0.125 0.625 0.25 0.0 0.0 0.125 0.625 0.25 0.0 0.0 0.0 0.125
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/validate_mapping_file_corrected_map.tabular Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,35 @@ +#SampleID BarcodeSequence LinkerPrimerSequence SampleType Year Month Day Subject ReportedAntibioticUsage DaysSinceExperimentStart Description +L1S8 AGCTGACTAGTC GTGCCAGCMGCCGCGGTAA gut 2008 10 28 1 Yes 0 1_Fece_10_28_2008 +L1S140 ATGGCAGCTCTA GTGCCAGCMGCCGCGGTAA gut 2008 10 28 2 Yes 0 2_Fece_10_28_2008 +L1S57 ACACACTATGGC GTGCCAGCMGCCGCGGTAA gut 2009 1 20 1 No 84 1_Fece_1_20_2009 +L1S208 CTGAGATACGCG GTGCCAGCMGCCGCGGTAA gut 2009 1 20 2 No 84 2_Fece_1_20_2009 +L1S76 ACTACGTGTGGT GTGCCAGCMGCCGCGGTAA gut 2009 2 17 1 No 112 1_Fece_2_17_2009 +L1S105 AGTGCGATGCGT GTGCCAGCMGCCGCGGTAA gut 2009 3 17 1 No 140 1_Fece_3_17_2009 +L1S257 CCGACTGAGATG GTGCCAGCMGCCGCGGTAA gut 2009 3 17 2 No 140 2_Fece_3_17_2009 +L1S281 CCTCTCGTGATC GTGCCAGCMGCCGCGGTAA gut 2009 4 14 2 No 168 2_Fece_4_14_2009 +L2S240 CATATCGCAGTT GTGCCAGCMGCCGCGGTAA left palm 2008 10 28 2 Yes 0 2_L_Palm_10_28_2008 +L2S155 ACGATGCGACCA GTGCCAGCMGCCGCGGTAA left palm 2009 1 20 1 No 84 1_L_Palm_1_20_2009 +L2S309 CGTGCATTATCA GTGCCAGCMGCCGCGGTAA left palm 2009 1 20 2 No 84 2_L_Palm_1_20_2009 +L2S175 AGCTATCCACGA GTGCCAGCMGCCGCGGTAA left palm 2009 2 17 1 No 112 1_L_Palm_2_17_2009 +L2S204 ATGCAGCTCAGT GTGCCAGCMGCCGCGGTAA left palm 2009 3 17 1 No 140 1_L_Palm_3_17_2009 +L2S357 CTAACGCAGTCA GTGCCAGCMGCCGCGGTAA left palm 2009 3 17 2 No 140 2_L_Palm_3_17_2009 +L2S222 CACGTGACATGT GTGCCAGCMGCCGCGGTAA left palm 2009 4 14 1 No 168 1_L_Palm_4_14_2009 +L2S382 CTCAATGACTCA GTGCCAGCMGCCGCGGTAA left palm 2009 4 14 2 No 168 2_L_Palm_4_14_2009 +L3S242 ACAGTTGCGCGA GTGCCAGCMGCCGCGGTAA right palm 2008 10 28 1 Yes 0 1_R_Palm_10_28_2008 +L3S378 ATCGATCTGTGG GTGCCAGCMGCCGCGGTAA right palm 2008 10 28 2 Yes 0 2_R_Palm_10_28_2008 +L3S294 CACGACAGGCTA GTGCCAGCMGCCGCGGTAA right palm 2009 1 20 1 No 84 1_R_Palm_1_20_2009 +L4S63 CTCGTGGAGTAG GTGCCAGCMGCCGCGGTAA right palm 2009 1 20 2 No 84 2_R_Palm_1_20_2009 +L3S313 AGTGTCACGGTG GTGCCAGCMGCCGCGGTAA right palm 2009 2 17 1 No 112 1_R_Palm_2_17_2009 +L3S341 CAAGTGAGAGAG GTGCCAGCMGCCGCGGTAA right palm 2009 3 17 1 No 140 1_R_Palm_3_17_2009 +L4S112 GCGTTACACACA GTGCCAGCMGCCGCGGTAA right palm 2009 3 17 2 No 140 2_R_Palm_3_17_2009 +L3S360 CATCGTATCAAC GTGCCAGCMGCCGCGGTAA right palm 2009 4 14 1 No 168 1_R_Palm_4_14_2009 +L4S137 GAACTGTATCTC GTGCCAGCMGCCGCGGTAA right palm 2009 4 14 2 No 168 2_R_Palm_4_14_2009 +L5S104 CAGTGTCAGGAC GTGCCAGCMGCCGCGGTAA tongue 2008 10 28 1 Yes 0 1_Tong_10_28_2008 +L5S240 CTGGACTCATAG GTGCCAGCMGCCGCGGTAA tongue 2008 10 28 2 Yes 0 2_Tong_10_28_2008 +L5S155 ATCTTAGACTGC GTGCCAGCMGCCGCGGTAA tongue 2009 1 20 1 No 84 1_Tong_1_20_2009 +L6S20 GAGGCTCATCAT GTGCCAGCMGCCGCGGTAA tongue 2009 1 20 2 No 84 2_Tong_1_20_2009 +L5S174 CAGACATTGCGT GTGCCAGCMGCCGCGGTAA tongue 2009 2 17 1 No 112 1_Tong_2_17_2009 +L5S203 CGATGCACCAGA GTGCCAGCMGCCGCGGTAA tongue 2009 3 17 1 No 140 1_Tong_3_17_2009 +L6S68 GATACGTCCTGA GTGCCAGCMGCCGCGGTAA tongue 2009 3 17 2 No 140 2_Tong_3_17_2009 +L5S222 CTAGAGACTCTT GTGCCAGCMGCCGCGGTAA tongue 2009 4 14 1 No 168 1_Tong_4_14_2009 +L6S93 GATTAGCACTCT GTGCCAGCMGCCGCGGTAA tongue 2009 4 14 2 No 168 2_Tong_4_14_2009
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/validate_mapping_file_log.txt Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,1 @@ +No errors or warnings found in mapping file. \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool-data/qiime_reference_db.loc.sample Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,32 @@ +#<unique_id> <database_caption> <fasta_file_path> +# +#For each reference database, you need to download the fasta file in qiime path +# +#List of useful db for qiime: http://qiime.org/home_static/dataFiles.html +# +#silva-119-90 SILVA v.119 90% $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set90_aligned.fna +#silva-119-90-16S SILVA v.119 90% (16S only) $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set90_aligned_16S_only.fna +#silva-119-90-18S SILVA v.119 90% (18S only) $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set90_aligned_18S_only.fna +#silva-119-94 SILVA v.119 94% $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set94_aligned.fna +#silva-119-94-16S SILVA v.119 94% (16S only) $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set94_aligned_16S_only.fna +#silva-119-94-18S SILVA v.119 94% (18S only) $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set94_aligned_18S_only.fna +#silva-119-97 SILVA v.119 97% $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set97_aligned.fna +#silva-119-97-16S SILVA v.119 97% (16S only) $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set97_aligned_16S_only.fna +#silva-119-97-18S SILVA v.119 97% (18S only) $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set97_aligned_18S_only.fna +#silva-119-99 SILVA v.119 99% $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set99_aligned.fna +#silva-119-99-16S SILVA v.119 99% (16S only) $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set99_aligned_16S_only.fna +#silva-119-99-18S SILVA v.119 99% (18S only) $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set99_aligned_18S_only.fna +#gg-13-8-61 Greengenes v.13.8 61% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_61.fasta +#gg-13-8-64 Greengenes v.13.8 64% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_64.fasta +#gg-13-8-67 Greengenes v.13.8 67% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_67.fasta +#gg-13-8-70 Greengenes v.13.8 70% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_70.fasta +#gg-13-8-73 Greengenes v.13.8 73% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_73.fasta +#gg-13-8-76 Greengenes v.13.8 76% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_76.fasta +#gg-13-8-79 Greengenes v.13.8 79% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_79.fasta +#gg-13-8-82 Greengenes v.13.8 82% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_82.fasta +#gg-13-8-85 Greengenes v.13.8 85% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_85.fasta +#gg-13-8-88 Greengenes v.13.8 88% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_88.fasta +#gg-13-8-91 Greengenes v.13.8 91% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_91.fasta +#gg-13-8-94 Greengenes v.13.8 94% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_94.fasta +#gg-13-8-97 Greengenes v.13.8 97% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_97.fasta +#gg-13-8-99 Greengenes v.13.8 99% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_99.fasta \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_data_table_conf.xml.sample Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,7 @@ +<tables> + <!-- Locations of qiime databases --> + <table name="qiime_reference_db" comment_char="#"> + <columns>value, name, path</columns> + <file path="tool-data/qiime_reference_db.loc" /> + </table> +</tables> \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_dependencies.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,6 @@ +<?xml version="1.0"?> +<tool_dependency> + <package name="qiime" version="1.9.1"> + <repository changeset_revision="73a3afb973b2" name="package_python_2_7_qiime_1_9_1" owner="iuc" toolshed="https://testtoolshed.g2.bx.psu.edu" /> + </package> +</tool_dependency>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/upgma_cluster.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,43 @@ +<tool id="qiime_upgma_cluster" name="upgma cluster" version="1.9.1galaxy1"> + <description>Build a UPGMA tree comparing samples</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <command> +<![CDATA[ + upgma_cluster.py + -i upgma_cluster_input + -o upgma_cluster_output +]]> + </command> + + <inputs> + <param label="-i/--input_path: input path. directory for batch processing, + filename for single file operation" name="input_path" type="data"/> + </inputs> + + <outputs> + <data format="newick" name="output_tree"/> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help><![CDATA[ +**What it does?** + +In addition to using PCoA, it can be useful to cluster samples using UPGMA (Unweighted Pair Group Method with Arithmetic mean, also known as average linkage). As with PCoA, the input to this step is a distance matrix (i.e. resulting file from beta_diversity.py). +The output is a newick formatted tree compatible with most standard tree viewing programs. Batch processing is also available, allowing the analysis of an entire directory of distance matrices. + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/validate_mapping_file.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,145 @@ +<tool id="qiime_validate_mapping_file" name="Validate mapping file" version="1.9.1"> + <description>to check for required data and format</description> + + <macros> + <import>macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <version_command><![CDATA[ + validate_mapping_file.py --version + ]]></version_command> + + <command><![CDATA[ + validate_mapping_file.py + -m $mapping_fp + -o validate_mapping_file_output + $verbose + -c $char_replace + $not_barcoded + $variable_len_barcodes + $disable_primer_check + + #if str($added_demultiplex_field): + -j $added_demultiplex_field + #end if + + $suppress_html + + #if not $suppress_html: + && + mkdir -p $html_report.files_path + && + cp validate_mapping_file_output/*.html $html_report.files_path + && + cp "validate_mapping_file_output/overlib.js" "$html_report.extra_files_path" + && + mv $html_report.files_path/*.html $html_report + #end if +]]> + </command> + + <inputs> + <param name="mapping_fp" label="Metadata mapping filepath" type="data" + format="tabular,csv,txt,tsv" help="(-m/--mapping_fp)"/> + + <param name="verbose" type="boolean" label="Enable printing information + to standard out?" truevalue="-v" falsevalue="" checked="False" + help="(-v/--verbose)" /> + + <param name="char_replace" type="text" value="_" label="Default character + used to replace invalid characters found in the mapping file" + help="Must be a valid character (alphanumeric, period, or underscore). + (-c/--char_replace)"/> + + <param name="not_barcoded" type="boolean" label="Are barcodes present?" + truevalue="" falsevalue="--not_barcoded" checked="True" + help="BarcodeSequence header is still required in mapping file + (-b/--not_barcoded)"/> + + <param name="variable_len_barcodes" type="boolean" label="Are barcodes with + variable length?" truevalue="" falsevalue="--variable_len_barcodes" + checked="True" help="(-B/--variable_len_barcodes)"/> + + <param name="disable_primer_check" type="boolean" label="Disable checks + for primers?" truevalue="-disable_primer_check" falsevalue="" + checked="False" help="LinkerPrimerSequence header is still required + in mapping file (-p/--disable_primer_check)"/> + + <param name="added_demultiplex_field" type="text" label="Field + to use in the mapping file as additional demultiplexing (optional)" + help="It can be used with or without barcodes. All combinations of + barcodes/primers and these fields must be unique. The fields must contain + values that can be parsed from the fasta labels such as 'plate=R_2008_12_09'. + In this case, 'plate' would be the column header and 'R_2008_12_09' + would be the field data (minus quotes) in the mapping file. + To use the run prefix from the fasta label, such as 'FLP3FBN01ELBSX', + where 'FLP3FBN01' is generated from the run ID, use 'run_prefix' and + set the run prefix to be used as the data under the column header + 'run_prefix' (-j/--added_demultiplex_field)" optional="True"/> + + <param name="suppress_html" type="boolean" label="Disable html file + generation?" truevalue="-s" falsevalue="" checked="False" + help="It can be useful for extremely large mapping files + (-s/--suppress_html)"/> + </inputs> + + <outputs> + <data name="html_report" format="html" + label="${tool.name} on ${on_string}: html report"> + <filter>suppress_html is False</filter> + </data> + <data name="log" format="txt" + from_work_dir="validate_mapping_file_output/*.log" + label="${tool.name} on ${on_string}: log"/> + <data format="tabular" name="mapping_fp_corrected" + from_work_dir="validate_mapping_file_output/*corrected.txt" + label="${tool.name} on ${on_string}: corrected map" /> + </outputs> + + <tests> + <test> + <param name="mapping_fp" value="map.tsv"/> + <param name="verbose" value="" /> + <param name="char_replace" value="_" /> + <param name="not_barcoded" value="" /> + <param name="variable_len_barcodes" value="" /> + <param name="disable_primer_check" value="" /> + <param name="suppress_html" value="" /> + + <output name="log" file="validate_mapping_file_log.txt"/> + <output name="mapping_fp_corrected" + file="validate_mapping_file_corrected_map.tabular"/> + </test> + </tests> + + <help><![CDATA[ +**What it does?** + +This tool checks user’s metadata mapping file for required data, valid format + +Specifically, it checks that: + + - The BarcodeSequence, LinkerPrimerSequences, and ReversePrimer fields have valid IUPAC DNA characters, and BarcodeSequence characters are non-degenerate (error) + - The SampleID, BarcodeSequence, LinkerPrimerSequence, and Description headers are present. (error) + - There are not duplicate header fields (error) + - There are not duplicate barcodes (error) + - Barcodes are of the same length. Suppressed when variable_len_barcode flag is passed (warning) + - The headers do not contain invalid characters (alphanumeric and underscore only) (warning) + - The data fields do not contain invalid characters (alphanumeric, underscore, space, and +-%./:,; characters) (warning) + - SampleID fields are MIENS compliant (only alphanumeric and . characters). (warning) + - There are no duplicates when the primer and variable length barcodes are appended (error) + - There are no duplicates when barcodes and added demultiplex fields (-j option) are combined (error) + - Data fields are not found beyond the Description column (warning) + +More information about this tool is available on +`QIIME documentation <http://qiime.org/scripts/validate_mapping_file.html>`_. + ]]> + </help> + + <citations> + <expand macro="citations" /> + </citations> + +</tool>