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annotate metaphlan2.xml @ 5:167876cbcdd5 draft

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planemo upload for repository https://github.com/ASaiM/galaxytools/tree/master/tools/sortmerna/ commit 259ec654231c792a102f99ab7bfd63cbabd4874a-dirty
author bebatut
date Wed, 28 Oct 2015 12:34:12 -0400
parents 575f1f4420ee
children 9603321a8519
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1 <tool id="metaphlan2" name="MetaPhlAn2" version="0.1.0">
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3 <description>to profile the composition of microbial communities</description>
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5 <requirements>
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6 <requirement type="package" version="2.2.5">bowtie2</requirement>
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7 <requirement type="package" version="2.0">metaphlan2</requirement>
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8 </requirements>
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10 <stdio>
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11 <exit_code range="1:" />
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12 </stdio>
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14 <version_command>
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15 <![CDATA[
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16 python \${METAPHLAN2_DIR}/metaphlan.py -v
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17 ]]>
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18 </version_command>
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20 <command><![CDATA[
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21 python \${METAPHLAN2_DIR}/metaphlan.py
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22 $input_file
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23 -o $output_file
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25 --input_type ${input_reads.datatype.file_ext}
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26 --mpa_pkl \${METAPHLAN2_DIR}/db_v20/mpa_v20_m200.pkl
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27 --bowtie2db \${METAPHLAN2_DIR}/db_v20/mpa_v20_m200
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29 $bowtie2_output
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30 #if $bowtie2_output == ''
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31 --bowtie2out $bowtie2_output
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32 #end if
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34 -t $analysis_type
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36 #if $analysis_type.analysis_type_select == "rel_ab"
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37 --tax_lev $analysis_type.taxonomic_level
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38 #else
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39 #if $analysis_type.analysis_type_select == "marker_ab_table"
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40 --nreads $analysis_type.nreads
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41 #else
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42 #if $analysis_type.analysis_type_select == "marker_pres_table"
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43 --pres_th $analysis_type.pres_th
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44 #end if
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45 #end if
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46 #end if
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47
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48 --min_cu_len $min_cu_len
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49 --min_alignment_len $min_alignment_len
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51 $ignore_viruses
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52 $ignore_eukaryotes
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53 $ignore_bacteria
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54 $ignore_archaea
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55
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56 --stat_q $stat_q
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57
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58 #if $sam_output
0
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59 -s $sam_output_file
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60 #endif
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61
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62 #if $biom_output
0
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63 -biom $biom_output_file
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64 #endif
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65
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66 ]]></command>
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67
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68 <inputs>
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69 <param name="input_file" type="data" format="fastq,fasta,sam,bowtie2out" label="Input file" help=""/>
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70
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71 <conditional name="analysis_type">
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72 <param name="analysis_type_select" type="select" label="Type of analysis to perform">
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73 <option value="rel_ab" selected="true">Profiling a metagenomes in terms of relative abundances</option>
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74 <option value="rel_ab_w_read_stats">Profiling a metagenomes in terms of relative abundances and estimate the number of reads comming from each clade</option>
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75 <option value="reads_map">Mapping from reads to clades (only reads hitting a marker)</option>
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76 <option value="clade_profiles">Normalized marker counts for clades with at least a non-null marker</option>
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77 <option value="marker_ab_table">Normalized marker counts (only when > 0.0 and normalized by metagenome size if --nreads is specified)</option>
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78 <option value="marker_pres_table">List of markers present in the sample (threshold at 1.0 if not differently specified with --pres_th</option>
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79 </param>
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80 <when value="rel_ab">
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81 <param name="taxonomic_level" type="select" label="Taxonomic level for the relative abundance output">
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82 <option value="a" selected="true">All taxonomic levels</option>
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83 <option value="k">Kingdoms (Bacteria and Archaea) only</option>
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84 <option value="p">Phyla only</option>
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85 <option value="c">Classes only</option>
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86 <option value="o">Orders only</option>
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87 <option value="f">Families only</option>
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88 <option value="g">Genera only</option>
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89 <option value="s">Species only</option>
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90 </param>
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91 </when>
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92 <when value="marker_ab_table">
1
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93 <param name="nreads" type="integer" value="0" label="Total number of reads in the original metagenome" help="It is used for normalizing the length-normalized counts with the metagenome size as well. No normalization applied if the value is not specified"/>
0
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94 </when>
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95 <when value="marker_pres_table">
1
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96 <param name="pres_th" type="integer" value="0" label=" Threshold for calling a marker present" help=""/>
0
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97 </when>
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98 </conditional>
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99
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100 <param name="min_cu_len" type="integer" value="2000" label="Minimum total nucleotide length for the markers in a clade for estimating the abundance without considering sub-clade abundances" help=""/>
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101
1
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102 <param name="min_alignment_len" type="integer" value="0" label="The sam records for aligned reads with the longest subalignment length smaller than this threshold will be discarded." help=""/>
0
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103
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104 <param name="ignore_viruses" type='boolean' checked="true" truevalue='' falsevalue='--ignore_viruses' label="Profile viral organisms?" help="" />
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105 <param name="ignore_eukaryotes" type='boolean' checked="true" truevalue='' falsevalue='--ignore_eukaryotes' label="Profile eukaryotic organisms?" help="" />
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106 <param name="ignore_bacteria" type='boolean' checked="true" truevalue='' falsevalue='--ignore_bacteria' label="Profile bacteria organisms?" help="" />
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107 <param name="ignore_archaea" type='boolean' checked="true" truevalue='' falsevalue='--ignore_archaea' label="Profile archea organisms?" help="" />
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108
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109 <param name="stat_q" type="float" value="0.1" label="Quantile value for the robust average" help=""/>
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110
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111 <param name="bowtie2_output" type='boolean' checked="true" truevalue='' falsevalue='--no_map' label="Store the output of BowTie2?" help="" />
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112 <param name="sam_output" type='boolean' checked="true" truevalue='yes' falsevalue='no' label="Output a sam file?" help="" />
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113 <param name="biom_output" type='boolean' checked="true" truevalue='yes' falsevalue='no' label="Output a biom file?" help="" />
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114 </inputs>
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115
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116 <outputs>
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117 <data format_source="txt" name="output_file"
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118 metadata="input_sequence_file"
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119 label="Profile of communities on ${on_string} (MetaPhlAn)" />
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120
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121 <data format_source="bowtie2" name="bowtie2_output_file"
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122 metadata="input_sequence_file"
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123 label="Bowtie2 output on ${on_string} (MetaPhlAn)">
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124 <filter>!bowtie2_output</filter>
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125 </data>
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126
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127 <data format_source="sam" name="sam_output_file"
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128 metadata="input_sequence_file"
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129 label="Sam output on ${on_string} (MetaPhlAn)">
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130 <filter>sam_output</filter>
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131 </data>
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132 <data format_source="biom" name="biom_output_file"
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133 metadata="input_sequence_file"
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134 label="Biom output on ${on_string} (MetaPhlAn)">
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135 <filter>biom_output</filter>
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136 </data>
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137 </outputs>
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138
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139 <tests>
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140 <test>
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141 <param name="input_file" value="input_sequences.fastq"/>
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142 <output name="output_file" file="profiled_metagenome.txt"/>
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143 </test>
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144 </tests>
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145
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146 <help><![CDATA[
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147
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148 **What it does**
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149
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150 MetaPhlAn is a computational tool for profiling the composition of microbial
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151 communities (Bacteria, Archaea, Eukaryotes and Viruses) from metagenomic shotgun
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152 sequencing data with species level resolution
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153
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154 .. _MetaPhlAn2 user manual: https://bitbucket.org/biobakery/metaphlan2
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155
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156 -----
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157
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158 **Input**
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159
1
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160 Metaphlan2 takes as input a sequence file in fasta, fastq, a BowTie2 produced
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161 SAM file or an intermediary mapping file of the metagenome generated by a
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162 previous MetaPhlAn
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163
0
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164 -----
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165
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166 **Parameters**
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167
1
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168 Several parameters can modulate the MetaPhlAn execution
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169
5
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170 * Mapping arguments
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171
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172 * Test to avoid saving the output of BowTie2
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173
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174 * Post-mapping arguments
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175
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176 * Taxonomic level for the relative abundance output
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177 * Minimum total nucleotide length for the markers in a clade for estimating the abundance without considering sub-clade abundances
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178 * Sam records for aligned reads with the longest subalignment length smaller than this threshold will be discarded
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179 * Tests to avoid profiling of virus, eukaryotes, bacteria and/or archea
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180 * Quantile value
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181
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182 * Additional analysis types and arguments
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183
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184 * Type of analyse to perform and some parameters for specific analysis type
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185
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186 -----
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187
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188 **Outputs**
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189
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190 The main output file is a tab-separated output file of the predicted taxon
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191 relative abundances.
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192
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193 Given the choosen parameters, other output files can be:
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194
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195 * a sam output file
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196 * a biom output fime
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197 * a BowTie2 output file
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198
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199 ]]></help>
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200
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201 <citations>
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202 <citation type="doi">10.1038/nmeth.3589</citation>
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203 </citations>
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204 </tool>