Mercurial > repos > abossers > mummer_toolsuite
annotate MUMmer/mummer_maxmatch.xml @ 0:59f302448cf6
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
author | abossers |
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date | Tue, 07 Jun 2011 17:22:27 -0400 |
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1 <tool id="mummer_maxmatch" name="MUMmer MaxMatch" version="0.9.alx" force_history_refresh="True"> |
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2 <description>: Maximal exact sequence matching</description> |
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3 <command> |
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4 <!-- update this path to the installed location --> |
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5 /opt/MUMmer/MUMmer/$tool.cmd |
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6 #if $tool.cmd=="mummer": |
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7 $tool.cmd_extra |
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8 $tool.mum_ref_in |
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9 $tool.mum_q_in |
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10 #end if |
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11 #if $tool.cmd=="repeat-match": |
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12 -n $tool.rm_n |
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13 #if $tool.rm_E=="yes": |
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14 -E |
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15 #end if |
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16 $tool.cmd_extra |
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17 $tool.in_seq |
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18 #end if |
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19 #if $tool.cmd=="exact-tandems": |
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20 $tool.in_seq |
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21 $tool.et_minl |
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22 #end if |
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23 <!-- unfortunate somehow error state gets set also on succesfull jobs. Pipe io stderr to dev/null --> |
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24 2>&- |
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25 > $out_tool |
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26 |
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27 </command> |
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28 <inputs> |
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29 <conditional name="tool"> |
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30 <param name="cmd" type="select" value="mummer" label="MUMmer maximal matching" help="Algorithms are run with default parameters (none). For specific args see help below" > |
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31 <option value="mummer">mummer</option> |
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32 <option value="repeat-match">repeat-match</option> |
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33 <option value="exact-tandems">exact-tandems</option> |
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34 </param> |
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35 <when value="mummer"> |
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36 <param name="mum_ref_in" type="data" format="fasta" label="Reference FastA file" /> |
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37 <param name="mum_q_in" type="data" format="fasta" label="Query (multi) FastA sequence" /> |
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38 <param name="cmd_extra" type="text" size="40" value="" label="Extra cmd line options" help="See specific cmd line options below for each tool" /> |
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39 </when> |
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40 <when value="repeat-match"> |
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41 <param name="in_seq" type="data" format="fasta" label="FastA sequence file" /> |
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42 <param name="rm_n" type="text" size="5" value="20" label="Minimum exact match length [-n]" /> |
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43 <param name="rm_E" type="select" value="no" label="Use exhaustive (slow) search to find matches [-E]" > |
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44 <option value="no">No</option> |
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45 <option value="yes">Yes</option> |
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46 </param> |
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47 <param name="cmd_extra" type="text" size="40" value="" label="Extra cmd line options" help="-n and -E are configured above. More specific cmd line options in help below." /> |
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48 </when> |
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49 <when value="exact-tandems"> |
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50 <param name="in_seq" type="data" format="fasta" label="FastA sequence file" /> |
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51 <param name="et_minl" type="text" size="5" value="20" label="Minimum length" /> |
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52 </when> |
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53 </conditional> |
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54 </inputs> |
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55 <outputs> |
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56 <data name="out_tool" format="text" label="Max exact match output" /> |
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57 </outputs> |
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58 <requirements> |
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59 <requirement type="binary">mummer</requirement> |
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60 <requirement type="binary">repeat-match</requirement> |
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61 <requirement type="binary">exact-tandems</requirement> |
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62 </requirements> |
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63 <tests> |
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64 <test> |
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65 </test> |
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66 </tests> |
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67 <help> |
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68 | |
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69 |
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70 |
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71 **Reference** |
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72 ============= |
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73 |
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74 - **MUMmer MaxExactMatch Galaxy tool wrapper:** Alex Bossers, CVI of Wageningen UR, The Netherlands. |
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75 |
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76 - **MUMmer suite v3.22:** http://mummer.sourceforge.net |
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77 |
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78 - **MUMmer tutorials:** http://mummer.sourceforge.net/examples/ |
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79 |
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80 Please do not use any of the command line options that modify prefixes or file names. As obvious |
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81 they are quite useless within galaxy and are likely to fail the routine! |
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82 |
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83 If you found these tools/wrappers usefull in your research, please acknowledge our work. If you improve |
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84 or modify the wrappers please add instead of substitute yourself into the acknowlegement section :) |
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85 |
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86 |
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87 |
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88 **MUMmer Maximal exact matching** |
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89 ================================= |
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90 |
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91 The heart of the MUMmer package is its suffix tree based maximal matching routines. These can be |
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92 used for repeat detection within a single sequence as is done by *repeat-match* and *exact-tandems*, |
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93 or can be used for the alignment of two or more sequences as is done by *mummer*. |
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94 |
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95 Mummer |
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96 ------ |
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97 |
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98 mummer is a suffix tree algorithm designed to find maximal exact matches of some minimum length |
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99 between two input sequences. by default mummer will only find maximal matches that are unique in |
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100 the entire set of reference sequences. The match lists produced by mummer can be used alone to |
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101 generate alignment dot plots, or can be passed on to the clustering algorithms for the identification |
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102 of longer non-exact regions of conservation. These match lists have great versatility because they |
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103 contain huge amounts of information and can be passed forward to other interpretation programs for |
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104 clustering, analysis, searching, etc. |
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105 |
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106 |
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107 Repeat-match |
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108 ------------ |
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109 |
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110 repeat-match is a suffix tree algorithm designed to find maximal exact repeats within a single input |
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111 sequence. It uses a similar algorithm to mummer, but altered slightly to find maximal exact matches |
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112 within a single sequence. |
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113 |
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114 Output formatting varies depending on the command line parameters and the output can be quite large. |
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115 The standard output format that results from running repeat-match with default parameters is as follows: |
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116 :: |
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117 |
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118 Long Exact Matches: |
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119 Start1 Start2 Length |
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120 4919485 4919506r 22 |
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121 |
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122 The three columns are the first position of the repeat, the second position of the repeat, and the |
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123 length of the repeat respectively. Reverse complement repeat positions are denoted by an 'r' |
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124 following the Start2 position, and are relative to the forward strand of the sequence. |
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125 |
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126 |
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127 Exact-tandems |
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128 ------------- |
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129 |
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130 exact-tandems is a wrapper script for the repeat-match program. It provides a list of exact tandem |
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131 repeats within a single input sequence. As with repeat-match the sequence file should contain only |
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132 one sequence in FastA format, however if multiple sequences exist the first one will be used. The |
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133 sequence may contain any set of upper and lowercase characters, thus DNA and protein sequence are |
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134 both allowed and matching is case insensitive. The minimum match length parameter should be a |
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135 positive integer, this value will be passed to the repeat-match program via the -n option. |
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136 |
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137 The output format of exact-tandems is as follows: |
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138 :: |
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139 |
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140 Finding matches |
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141 Tandem repeats |
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142 Start Extent UnitLen Copies |
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143 416173 150 45 3.3 |
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144 |
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145 The four columns are the first position of the tandem, the extent of the repeat region, the length |
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146 of each tandem repeat unit, and the number of repeat units respectively. |
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147 |
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148 |
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149 |
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150 **Manuals and CMD line options (specific for each tool!):** |
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151 =========================================================== |
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152 |
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153 **Mummer** |
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154 |
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155 http://mummer.sourceforge.net/manual/#mummer |
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156 |
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157 **Repeat-match** |
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158 |
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159 http://mummer.sourceforge.net/manual/#repeat |
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160 |
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161 **exact-tandems** |
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162 |
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163 http://mummer.sourceforge.net/manual/#exact |
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164 |
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165 | |
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166 | |
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167 |
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168 </help> |
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169 </tool> |
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170 |