diff hubArchiveCreator.xml @ 1:85195e0d4b71 draft

planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit b1ae7349f118a0fe7923d765020dfc684cf84116-dirty
author yating-l
date Fri, 29 Sep 2017 13:32:23 -0400
parents
children fa990284327b
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/hubArchiveCreator.xml	Fri Sep 29 13:32:23 2017 -0400
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+<tool id="hubArchiveCreator" name="Hub Archive Creator" version="2.5.0">
+    <description>
+        This Galaxy tool permits to prepare your files to be ready for
+        Assembly Hub visualization.
+    </description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+
+    <requirements>
+        <requirement type="package" version="340">ucsc_hac</requirement>
+        <requirement type="package" version="1.2">samtools</requirement>
+        <requirement type="package" version="340">ucsc_bigwig</requirement>
+        <requirement type="package" version="340">ucsc_bigbed</requirement>
+    </requirements>
+
+    <stdio>
+        <regex match="^pass1"
+               source="stderr"
+               level="log"
+               description="bedToBigBed"/>
+        <!-- TODO: Add the case pass1 and 0 chroms -->
+        <!-- TODO: Add the case pass2 and 0 records or 0 fields -->
+    </stdio>
+
+    <!-- Idea: python \ -augustus [parameters] \ -trfBig [parameters] -->
+    <command detect_errors="exit_code"><![CDATA[
+        mkdir -p $output.extra_files_path;
+        
+        ## Dump the tool parameters into a JSON file 
+        python $json_file parameters.json;
+
+        python $__tool_directory__/hubArchiveCreator.py --data_json parameters.json -o $output;
+
+
+    ]]></command>
+    <configfiles>
+        <configfile name="json_file">
+import json
+import sys
+
+file_path = sys.argv[1]
+#set global $data_parameter_dict = {}
+
+## Ask the user to enter the genome name
+#silent $data_parameter_dict.update({"genome_name": str($genome_name)})
+
+## Function to retrieve the data of the inputs
+#def prepare_json($datatype, $input_to_prepare, $order_index, $extra_data_dict={})
+    #set false_path = str($input_to_prepare)
+    #set $data_dict = {"false_path": $false_path}
+
+    #set name = str($input_to_prepare.name)
+    #silent $data_dict.update({"name": $name})
+    #silent $data_dict.update($extra_data_dict)
+    ## Add the ordering by taking the tool form indexes
+    #silent $data_dict.update({"order_index": $order_index})
+
+    #if $datatype in $data_parameter_dict
+        #silent $data_parameter_dict[$datatype].append($data_dict)
+    #else
+        #set array_inputs = []
+        #silent $array_inputs.append($data_dict)
+        #silent $data_parameter_dict.update({$datatype: $array_inputs})
+    #end if 
+#end def
+
+## Get the number of digits from tracks, to have a unique integer from group index and track index
+
+#set temp_max_digit = 0
+
+#for $g in $group
+    #if len($g.format) > $temp_max_digit
+        #silent temp_max_digit = len($g.format)
+    #end if
+#end for
+
+
+#set nb_digits_max_track = len(str($temp_max_digit))
+
+## END Get the number of digits
+
+#for $i_g, $g in enumerate( $group )
+    #for $i, $f in enumerate( $g.format )
+        ## Create the order index using index_group+1 concatenated with index_track
+        #set index_group_final = str($i_g + 1)
+        #set index_track_final = str($index_group_final) + str($i).zfill($nb_digits_max_track)
+
+        ## For each format, we have a few mandatory fields we store in a dict
+        #set track_color = str($f.formatChoice.track_color)
+        #set group_name = str($g.group_name)
+        #set longLabel = str($f.formatChoice.longLabel)
+        #set extra_data_dict = {"track_color": $track_color,
+                                "group_name": $group_name,
+                                "long_label": $longLabel}
+
+        #if $f.formatChoice.format_select == "bam"
+            #set bam_index = $f.formatChoice.BAM.metadata.bam_index
+
+            ## Add Bam format specific fields
+            #silent $extra_data_dict.update({"index": $bam_index})
+
+            #silent $prepare_json("Bam", $f.formatChoice.BAM, $index_track_final, $extra_data_dict)
+        #end if
+        #if $f.formatChoice.format_select == "bed"
+            #if $f.formatChoice.bedChoice.bed_select == "bed_generic"
+                #silent $prepare_json("Bed", $f.formatChoice.bedChoice.BED, $index_track_final,
+                                                $extra_data_dict)
+            #end if
+            #if $f.formatChoice.bedChoice.bed_select == "bed_cytoBand"
+                #silent $prepare_json("CytoBand", $f.formatChoice.bedChoice.BED_cytoBand, $index_track_final,
+                                                $extra_data_dict)
+            #end if
+            #if $f.formatChoice.bedChoice.bed_select == "bed_simple_repeats_option"
+                #silent $prepare_json("BedSimpleRepeats", $f.formatChoice.bedChoice.BED_simple_repeats, $index_track_final,
+                                                $extra_data_dict)
+            #end if
+            #if $f.formatChoice.bedChoice.bed_select == "bed_splice_junctions_option"
+                #silent $prepare_json("BedSpliceJunctions", $f.formatChoice.bedChoice.BED_splice_junctions, $index_track_final,
+                                                $extra_data_dict)
+            #end if
+            #if $f.formatChoice.bedChoice.bed_select == "bed_blast_alignment_option"
+                #set database = str($f.formatChoice.bedChoice.database)
+                #silent $extra_data_dict.update({"database": $database})
+                #if $f.formatChoice.bedChoice.add_trix_index.add_trix_index_selector == "yes"
+                    #set trix_id = str($f.formatChoice.bedChoice.add_trix_index.trix_id)
+                    #silent $extra_data_dict.update({"trix_id": $trix_id})
+                    #if $f.formatChoice.bedChoice.add_trix_index.index_files.index_files_type == "collection"
+                        #set ix_index = str($f.formatChoice.bedChoice.add_trix_index.index_files.trix_index.index_ix)
+                        #set ixx_index = str($f.formatChoice.bedChoice.add_trix_index.index_files.trix_index.index_ixx)
+                    #else 
+                        #set ix_index = str($f.formatChoice.bedChoice.add_trix_index.index_files.index_ix)
+                        #set ixx_index = str($f.formatChoice.bedChoice.add_trix_index.index_files.index_ixx)
+                    #end if
+                    #silent $extra_data_dict.update({"indexIx": $ix_index, "indexIxx": $ixx_index})
+                #end if
+                #silent $prepare_json("BedBlastAlignments", $f.formatChoice.bedChoice.BED_blast_alignment, $index_track_final,
+                                             $extra_data_dict)
+            #end if
+            #if $f.formatChoice.bedChoice.bed_select == "bed_blat_alignment_option"
+                #set database = str($f.formatChoice.bedChoice.database)
+                #silent $extra_data_dict.update({"database": $database})
+                #if $f.formatChoice.bedChoice.add_trix_index.add_trix_index_selector == "yes"
+                    #set trix_id = str($f.formatChoice.bedChoice.add_trix_index.trix_id)
+                    #silent $extra_data_dict.update({"trix_id": $trix_id})
+                    #if $f.formatChoice.bedChoice.add_trix_index.index_files.index_files_type == "collection"
+                        #set ix_index = str($f.formatChoice.bedChoice.add_trix_index.index_files.trix_index.index_ix)
+                        #set ixx_index = str($f.formatChoice.bedChoice.add_trix_index.index_files.trix_index.index_ixx)
+                    #else 
+                        #set ix_index = str($f.formatChoice.bedChoice.add_trix_index.index_files.index_ix)
+                        #set ixx_index = str($f.formatChoice.bedChoice.add_trix_index.index_files.index_ixx)
+                    #end if
+                    #silent $extra_data_dict.update({"indexIx": $ix_index, "indexIxx": $ixx_index})
+                #end if
+                #silent $prepare_json("BedBlatAlignments", $f.formatChoice.bedChoice.BED_blat_alignment, $index_track_final,
+                                             $extra_data_dict)
+            #end if
+        #end if
+        #if $f.formatChoice.format_select == "psl"
+            #silent $prepare_json("Psl", $f.formatChoice.PSL, $index_track_final,
+                                           $extra_data_dict)
+        #end if
+        #if $f.formatChoice.format_select == "bigwig"
+            #silent $prepare_json("BigWig", $f.formatChoice.BIGWIG, $index_track_final,
+                                            $extra_data_dict)
+        #end if
+        #if $f.formatChoice.format_select == "bigbed"
+            #if $f.formatChoice.add_trix_index.add_trix_index_selector == "yes"
+                    #set trix_id = str($f.formatChoice.add_trix_index.trix_id)
+                    #silent $extra_data_dict.update({"trix_id": $trix_id})
+                    #if $f.formatChoice.add_trix_index.index_files.index_files_type == "collection"
+                        #set ix_index = str($f.formatChoice.add_trix_index.index_files.trix_index.index_ix)
+                        #set ixx_index = str($f.formatChoice.add_trix_index.index_files.trix_index.index_ixx)
+                    #else 
+                        #set ix_index = str($f.formatChoice.add_trix_index.index_files.index_ix)
+                        #set ixx_index = str($f.formatChoice.add_trix_index.index_files.index_ixx)
+                    #end if
+                    #silent $extra_data_dict.update({"indexIx": $ix_index, "indexIxx": $ixx_index})
+            #end if
+            #silent $prepare_json("BigBed", $f.formatChoice.BIGBED, $index_track_final,
+                                            $extra_data_dict)
+        #end if
+        #if $f.formatChoice.format_select == "gff3"
+            #silent $prepare_json("Gff3", $f.formatChoice.GFF3, $index_track_final,
+                                            $extra_data_dict)
+        #end if
+        #if $f.formatChoice.format_select == "gtf"
+            ## Add also GTF from Agustus? See https://github.com/ENCODE-DCC/kentUtils/issues/8
+            #silent $prepare_json("Gtf", $f.formatChoice.GTF, $index_track_final,
+                                             $extra_data_dict)
+        #end if
+    #end for
+#end for
+
+## We combine the fasta file dataset name with his false path in a JSON object
+#set fasta_json = {"false_path": str($fasta_file), "name": str($fasta_file.name)}
+$data_parameter_dict.update({"fasta": $fasta_json})
+
+## Retrieve the user email
+#silent $data_parameter_dict.update({"user_email": str($__user_email__)})
+
+#silent $data_parameter_dict.update({"tool_directory": str($__tool_directory__)})
+
+#silent $data_parameter_dict.update({"extra_files_path": str($output.extra_files_path)})
+
+#silent $data_parameter_dict.update({"debug_mode": str($advanced_options.debug_mode)})
+
+with open(file_path, 'w') as f:
+    json.dump($data_parameter_dict, f)
+
+        </configfile>
+    </configfiles>
+
+    <inputs>
+        <param
+                name="genome_name"
+                type="text"
+                size="30"
+                value="unknown"
+                label="UCSC Genome Browser assembly ID"
+        />
+        <param
+                format="fasta"
+                name="fasta_file"
+                type="data"
+                label="Reference genome"
+        />
+        <repeat name="group" title="New group">
+            <param type="text" name="group_name" label="Group name" value="Default group"/>
+            <repeat name="format" title="New track">
+                <conditional name="formatChoice">
+                    <param name="format_select" type="select" label="Format">
+                        <option value="bam" selected="true">BAM</option>
+                        <option value="bed">BED</option>
+                        <option value="psl">PSL</option>
+                        <option value="bigwig">BIGWIG</option>
+                        <option value="bigbed">BIGBED</option>
+                        <option value="gff3">GFF3</option>
+                        <option value="gtf">GTF</option>
+                    </param>
+
+                    <when value="bam">
+                        <param
+                                format="bam"
+                                name="BAM"
+                                type="data"
+                                label="BAM File"
+                        />
+                        <param name="longLabel" type="text" size="76" label="Track label" help="It is limited to 76 printable characters, the first 17 printable characters will be used as a short label of the track" />
+                        <!-- TODO: Find a solution to avoid repetition and to generate a new color depending on the others -->
+                        <param name="track_color" type="color" label="Track color" value="#000000">
+                            <sanitizer>
+                                <valid initial="string.letters,string.digits">
+                                    <add value="#"/>
+                                </valid>
+                            </sanitizer>
+                        </param>
+                    </when>
+                    <when value="bed">
+                        <conditional name="bedChoice">
+                            <param name="bed_select" type="select" label="Bed Choice">
+                                <option value="bed_generic" selected="true">BED Generic (bed4)</option>
+                                <option value="bed_cytoBand">cytoBand Track (bed4+1)</option>
+                                <option value="bed_simple_repeats_option">BED Simple repeat (bed4+12 / simpleRepeat.as)</option>
+                                <option value="bed_splice_junctions_option">BED Splice junctions (bed12+1 / spliceJunctions.as)</option>
+                                <option value="bed_blast_alignment_option">Blast alignments (bed12+12 / bigPsl.as)</option>
+                                <option value="bed_blat_alignment_option">BLAT alignments (bigPsl / bigPsl.as)</option>
+                            </param>
+                            <when value="bed_generic">
+                                <param
+                                        format="bed"
+                                        name="BED"
+                                        type="data"
+                                        label="Generic Bed File Choice"
+                                />
+
+                            </when>
+                            <when value="bed_cytoBand">
+                                <param
+                                        format="bed"
+                                        name="BED_cytoBand"
+                                        type="data"
+                                        label="cytoBand Track (Bed4)"
+                                />
+                            </when>
+                            <when value="bed_simple_repeats_option">
+                                <param
+                                        format="bed"
+                                        name="BED_simple_repeats"
+                                        type="data"
+                                        label="Bed Simple Repeats (Bed4+12) File"
+                                />
+                            </when>
+                            <when value="bed_splice_junctions_option">
+                                <param
+                                        format="bed"
+                                        name="BED_splice_junctions"
+                                        type="data"
+                                        label="Bed Splice Junctions (Bed12+1) File"
+                                />
+                            </when>
+                            <when value="bed_blast_alignment_option">
+                                <param
+                                        format="bed"
+                                        name="BED_blast_alignment"
+                                        type="data"
+                                        label="Bed Blast Alignments (Bed12+12) File"
+                                />
+                                <param name="database" type="select" label="Protein database">
+                                    <option value="NCBI" selected="true">NCBI</option>
+                                    <option value="UniProt">UniProt</option>
+                                    <option value="FlyBase">FlyBase</option>
+                                    <option value="Others">Others</option>
+                                </param>
+                                <expand macro="add_trix_file"/>
+                            </when>
+                            <when value="bed_blat_alignment_option">
+                                <param
+                                        format="bed"
+                                        name="BED_blat_alignment"
+                                        type="data"
+                                        label="Bed BLAT Alignments (bigPsl) File"
+                                />
+                                <param name="database" type="select" label="mRNA database">
+                                    <option value="NCBI" selected="true">NCBI</option>
+                                    <option value="Others">Others</option>
+                                </param>
+                                <expand macro="add_trix_file"/>
+                            </when>
+                        </conditional>
+                        <param name="longLabel" type="text" size="76" label="Track label" help="It is limited to 76 printable characters, the first 17 printable characters will be used as a short label of the track" />
+                        <param name="track_color" type="color" label="Track color" value="#000000">
+                            <sanitizer>
+                                <valid initial="string.letters,string.digits">
+                                    <add value="#"/>
+                                </valid>
+                            </sanitizer>
+                        </param>
+                    </when>
+                    <when value="psl">
+                        <param
+                                format="psl"
+                                name="PSL"
+                                type="data"
+                                label="PSL File"
+                        />
+                        <param name="longLabel" type="text" size="76" label="Track label" help="It is limited to 76 printable characters, the first 17 printable characters will be used as a short label of the track" />
+                        <param name="track_color" type="color" label="Track color" value="#000000">
+                            <sanitizer>
+                                <valid initial="string.letters,string.digits">
+                                    <add value="#"/>
+                                </valid>
+                            </sanitizer>
+                        </param>
+                    </when>
+                    <when value="bigwig">
+                        <param
+                                format="bigwig"
+                                name="BIGWIG"
+                                type="data"
+                                label="BIGWIG File"
+                        />
+                        <param name="longLabel" type="text" size="76" label="Track label" help="It is limited to 76 printable characters, the first 17 printable characters will be used as a short label of the track" />
+                        <param name="track_color" type="color" label="Track color" value="#000000">
+                            <sanitizer>
+                                <valid initial="string.letters,string.digits">
+                                    <add value="#"/>
+                                </valid>
+                            </sanitizer>
+                        </param>
+                    </when>
+                    <when value="bigbed">
+                        <param
+                                format="bigbed"
+                                name="BIGBED"
+                                type="data"
+                                label="BIGBED File"
+                        />
+                        <expand macro="add_trix_file">
+                            <expand macro="specify_search_index" />
+                        </expand>
+                        <param name="longLabel" type="text" size="76" label="Track label" help="It is limited to 76 printable characters, the first 17 printable characters will be used as a short label of the track" />
+                        <param name="track_color" type="color" label="Track color" value="#000000">
+                            <sanitizer>
+                                <valid initial="string.letters,string.digits">
+                                    <add value="#"/>
+                                </valid>
+                            </sanitizer>
+                        </param>
+                    </when>
+                    <when value="gff3">
+                        <param
+                                format="gff3"
+                                name="GFF3"
+                                type="data"
+                                label="GFF3 File"
+                        />
+                        <param name="longLabel" type="text" size="76" label="Track label" help="It is limited to 76 printable characters, the first 17 printable characters will be used as a short label of the track" />
+                        <param name="track_color" type="color" label="Track color" value="#000000">
+                            <sanitizer>
+                                <valid initial="string.letters,string.digits">
+                                    <add value="#"/>
+                                </valid>
+                            </sanitizer>
+                        </param>
+                    </when>
+                    <when value="gtf">
+                        <param
+                                format="gtf"
+                                name="GTF"
+                                type="data"
+                                label="GTF File"
+                        />
+                        <param name="longLabel" type="text" size="76" label="Track label" help="It is limited to 76 printable characters, the first 17 printable characters will be used as a short label of the track" />
+                        <param name="track_color" type="color" label="Track color" value="#000000">
+                            <sanitizer>
+                                <valid initial="string.letters,string.digits">
+                                    <add value="#"/>
+                                </valid>
+                            </sanitizer>
+                        </param>
+                    </when>
+                </conditional>
+            </repeat>
+        </repeat>
+        <conditional name="advanced_options">
+            <param name="advanced_options_selector" type="select" label="Advanced options">
+                <option value="off" selected="true">Hide advanced options</option>
+                <option value="on">Display advanced options</option>
+            </param>
+            <!-- TODO: Avoid redundancy here -->
+            <when value="on">
+                <param name="debug_mode" type="select" label="Activate debug mode"> 
+                    <option value="false" selected="true">No</option>
+                    <option value="true">Yes</option>
+                    <help>
+                        Use this option if you are a G-OnRamp developer
+                    </help>
+                </param>
+            </when>
+            <when value="off">
+                <param name="debug_mode" type="hidden"
+                       value="false">
+                </param>
+            </when>
+        </conditional>
+    </inputs>
+
+    <outputs>
+        <data format="trackhub" name="output"/>
+    </outputs>
+
+    <tests>
+        <!-- Test with only the fasta file -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <output name="output" file="only_genome/only_genome.html">
+                <!-- Use macro to check the whole common structure without repeated code -->
+                <expand macro="verify_hub_structure_no_track" test="only_genome" />
+            </output>
+        </test>
+
+        <!-- Test with Bam -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bam"/>
+                        <param name="BAM" value="bam/inputs/HISAT2_Accepted_Hits.bam" />
+                        <param name="longLabel" value="HISAT sequence alignment" />
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="bam/bam.html">
+                <!-- Verify tracks folder contains bam and bai -->
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam"
+                             value="bam/myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam"
+                             compare="sim_size"
+                />
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam.bai"
+                             value="bam/myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam.bai"
+                             compare="sim_size"
+                />
+                <!-- Use macro to check the whole common structure without repeated code -->
+                <expand macro="verify_hub_structure" test="bam" />
+            </output>
+        </test>
+
+        <!-- Test with Bed Generic -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bed" />
+                        <conditional name="bedChoice">
+                            <param name="bed_select" value="bed_generic"/>
+                            <param name="BED" ftype="bed" value="bed_generic/inputs/TBLASTN_Alignment_to_proteins"/>
+                            <param name="longLabel" value="TBLASTN alignment" />
+                            <param name="track_color" value="#000000"/>
+                        </conditional>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="bed_generic/bed_generic.html">
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/TBLASTN_Alignment_to_proteins.bb"
+                             value="bed_generic/myHub/Dbia3/tracks/TBLASTN_Alignment_to_proteins.bb"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="bed_generic" />
+            </output>
+        </test>
+
+        <!-- Test with Bed Simple repeat -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bed" />
+                        <conditional name="bedChoice">
+                            <param name="bed_select" value="bed_simple_repeats_option"/>
+                            <param name="BED_simple_repeats" ftype="bed" value="bed_simple_repeats/inputs/Repeating_Elements_by_TrfBig"/>
+                            <param name="longLabel" value="Simple repeat" />
+                            <param name="track_color" value="#000000"/>
+                        </conditional>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="bed_simple_repeats/bed_simple_repeats_trackhub.html">
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/Repeating_Elements_by_TrfBig.bb"
+                             value="bed_simple_repeats/myHub/Dbia3/tracks/Repeating_Elements_by_TrfBig.bb"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="bed_simple_repeats" />
+            </output>
+        </test>
+
+        <!-- Test with Psl -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="psl"/>
+                        <param name="PSL" value="psl/inputs/blastXmlToPsl"/>
+                        <param name="longLabel" value="BLAST Alignment" />
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="psl/psl_trackhub.html">
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/blastXmlToPsl.bb"
+                             value="psl/myHub/Dbia3/tracks/blastXmlToPsl.bb"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="psl" />
+            </output>
+        </test>
+
+        <!-- Test with BigWig -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bigwig"/>
+                        <param name="BIGWIG" value="bigwig/inputs/RNA-Seq_Alignment_Coverage"/>
+                        <param name="longLabel" value="RNA-Seq Coverage" />
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="bigwig/bigwig.html">
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/RNA-Seq_Alignment_Coverage.bigwig"
+                             value="bigwig/myHub/Dbia3/tracks/RNA-Seq_Alignment_Coverage.bigwig"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="bigwig" />
+                <!-- check additional trackDb settings for BigWig -->
+                <extra_files type="file" name="myHub/Dbia3/trackDb.txt">
+                    <assert_contents>
+                        <has_text text="autoScale"/>
+                        <has_text text="maxHeightPixels"/>
+                        <has_text text="windowingFunction"/>
+                    </assert_contents>
+                </extra_files>
+            </output>
+        </test>
+
+        <!-- Test with GFF3 -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="gff3"/>
+                        <param name="GFF3" value="gff3/inputs/Augustus_Gene_Predictions"/>
+                        <param name="longLabel" value="Augustus" />
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="gff3/gff3_trackhub.html">
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/Augustus_Gene_Predictions.bb"
+                             value="gff3/myHub/Dbia3/tracks/Augustus_Gene_Predictions.bb"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="gff3" />
+            </output>
+        </test>
+
+        <!-- Test with GTF -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="gtf"/>
+                        <param name="GTF" value="gtf/inputs/StringTie_Assembled_Transcripts"/>
+                        <param name="longLabel" value="StringTie transcripts" />
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="gtf/gtf_trackhub.html">
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb"
+                             value="gtf/myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="gtf" />
+            </output>
+        </test>
+
+        <!-- Test with BLAT Alignment -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bed" />
+                        <conditional name="bedChoice">
+                            <param name="bed_select" value="bed_blat_alignment_option"/>
+                            <param name="BED_blat_alignment" ftype="bed" value="bed_blat_alignment/inputs/BLAT_alignment_bigpsl"/>
+                            <param name="longLabel" value="BLAT alignment" />
+                            <param name="track_color" value="#000000"/>
+                            <param name="database" value="NCBI" />
+                        </conditional>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="bed_blat_alignment/blat_alignment_trackhub.html">
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/BLAT_alignment_bigpsl.bb"
+                             value="bed_blat_alignment/myHub/Dbia3/tracks/BLAT_alignment_bigpsl.bb"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="bed_blat_alignment" />
+                <!-- check additional trackDb settings for BLAT -->
+                <extra_files type="file" name="myHub/Dbia3/trackDb.txt">
+                    <assert_contents>
+                        <has_text text="searchIndex"/>
+                        <has_text text="url"/>
+                        <has_text text="urlLabel"/>
+                        <has_text text="iframeUrl"/>
+                        <has_text text="iframeOptions"/>
+                    </assert_contents>
+                </extra_files>
+            </output>
+        </test>
+
+        <!-- Test with BLAST Alignment -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bed" />
+                        <conditional name="bedChoice">
+                            <param name="bed_select" value="bed_blast_alignment_option"/>
+                            <param name="BED_blast_alignment" ftype="bed" value="bed_blast_alignment/inputs/BLAST_alignment_bigpsl"/>
+                            <param name="longLabel" value="BLAST alignment" />
+                            <param name="track_color" value="#000000"/>
+                            <param name="database" value="NCBI" />
+                        </conditional>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="bed_blast_alignment/blast_alignment_trackhub.html">
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/BLAST_alignment_bigpsl.bb"
+                             value="bed_blast_alignment/myHub/Dbia3/tracks/BLAST_alignment_bigpsl.bb"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="bed_blast_alignment" />
+                <!-- check additional trackDb settings for BLAST -->
+                <extra_files type="file" name="myHub/Dbia3/trackDb.txt">
+                    <assert_contents>
+                        <has_text text="searchIndex"/>
+                        <has_text text="url"/>
+                        <has_text text="urlLabel"/>
+                        <has_text text="iframeUrl"/>
+                        <has_text text="iframeOptions"/>
+                    </assert_contents>
+                </extra_files>
+            </output>
+        </test>
+
+        <!-- Test with Regtools Splice Junctions -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bed" />
+                        <conditional name="bedChoice">
+                            <param name="bed_select" value="bed_splice_junctions_option"/>
+                            <param name="BED_splice_junctions" ftype="bed" value="bed_splice_junctions/inputs/regtools_junctions"/>
+                            <param name="longLabel" value="Splice junctions" />
+                            <param name="track_color" value="#000000"/>
+                        </conditional>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="bed_splice_junctions/splice_junctions_trackhub.html">
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/regtools_junctions.bb"
+                             value="bed_splice_junctions/myHub/Dbia3/tracks/regtools_junctions.bb"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="bed_splice_junctions" />
+            </output>
+        </test>
+
+        <!-- Test with Cytoband -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bed" />
+                        <conditional name="bedChoice">
+                            <param name="bed_select" value="bed_cytoBand"/>
+                            <param name="BED_cytoBand" ftype="bed" value="bed_cytoband/inputs/Cytoband"/>
+                            <param name="longLabel" value="Cytoband" />
+                            <param name="track_color" value="#000000"/>
+                        </conditional>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="bed_cytoband/cytoband_trackhub.html">
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/Cytoband.bb"
+                             value="bed_cytoband/myHub/Dbia3/tracks/Cytoband.bb"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="bed_cytoband" />
+                <extra_files type="file" name="myHub/Dbia3/trackDb.txt">
+                    <assert_contents>
+                        <has_text text="track cytoBandIdeo"/>
+                    </assert_contents>
+                </extra_files>
+            </output>
+        </test>
+
+        <!-- Test with bigBed -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bigbed"/>
+                        <param name="BIGBED" value="big_bed/inputs/BLAT_alignment_bigbed" />
+                        <param name="longLabel" value="bigBed" />
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="big_bed/bigbed_trackhub.html">
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/BLAT_alignment_bigbed.bigbed"
+                             value="big_bed/myHub/Dbia3/tracks/BLAT_alignment_bigbed.bigbed"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="big_bed" />
+            </output>
+        </test>
+
+        <!-- Test with one group and multiple tracks -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="gtf"/>
+                        <param name="GTF" value="gtf/inputs/StringTie_Assembled_Transcripts"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="gff3"/>
+                        <param name="GFF3" value="gff3/inputs/Augustus_Gene_Predictions"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="gtf_gff/gtf_gff_trackhub.html">
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb"
+                             value="gtf/myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb"
+                             compare="sim_size"
+                />
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/Augustus_Gene_Predictions.bb"
+                             value="gff3/myHub/Dbia3/tracks/Augustus_Gene_Predictions.bb"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="gtf_gff" />   
+            </output>
+        </test>
+
+        <!-- Test with one group and all the supported datatypes -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bam"/>
+                        <param name="BAM" value="bam/inputs/HISAT2_Accepted_Hits.bam"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bed"/>
+                        <conditional name="bedChoice">
+                            <param name="bed_select" value="bed_generic"/>
+                            <param name="BED" ftype="bed" value="bed_generic/inputs/TBLASTN_Alignment_to_proteins"/>
+                            <param name="track_color" value="#000000"/>
+                        </conditional>
+                    </conditional>
+                </repeat>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bed"/>
+                        <conditional name="bedChoice">
+                            <param name="bed_select" value="bed_simple_repeats_option"/>
+                            <param name="BED_simple_repeats" ftype="bed"
+                                   value="bed_simple_repeats/inputs/Repeating_Elements_by_TrfBig"/>
+                            <param name="track_color" value="#000000"/>
+                        </conditional>
+                    </conditional>
+                </repeat>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="psl"/>
+                        <param name="PSL" value="psl/inputs/blastXmlToPsl"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bigwig"/>
+                        <param name="BIGWIG" value="bigwig/inputs/RNA-Seq_Alignment_Coverage"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="gff3"/>
+                        <param name="GFF3" value="gff3/inputs/Augustus_Gene_Predictions"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="gtf"/>
+                        <param name="GTF" value="gtf/inputs/StringTie_Assembled_Transcripts"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bed" />
+                        <conditional name="bedChoice">
+                            <param name="bed_select" value="bed_cytoBand"/>
+                            <param name="BED_cytoBand" ftype="bed" value="bed_cytoband/inputs/Cytoband"/>
+                            <param name="track_color" value="#000000"/>
+                        </conditional>
+                    </conditional>
+                </repeat>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bed" />
+                        <conditional name="bedChoice">
+                            <param name="bed_select" value="bed_splice_junctions_option"/>
+                            <param name="BED_splice_junctions" ftype="bed" value="bed_splice_junctions/inputs/regtools_junctions"/>
+                            <param name="track_color" value="#000000"/>
+                        </conditional>
+                    </conditional>
+                </repeat>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bed" />
+                        <conditional name="bedChoice">
+                            <param name="bed_select" value="bed_blast_alignment_option"/>
+                            <param name="BED_blast_alignment" ftype="bed" value="bed_blast_alignment/inputs/BLAST_alignment_bigpsl"/>
+                            <param name="track_color" value="#000000"/>
+                            <param name="database" value="NCBI" />
+                        </conditional>
+                    </conditional>
+                </repeat>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bed" />
+                        <conditional name="bedChoice">
+                            <param name="bed_select" value="bed_blat_alignment_option"/>
+                            <param name="BED_blat_alignment" ftype="bed" value="bed_blat_alignment/inputs/BLAT_alignment_bigpsl"/>
+                            <param name="track_color" value="#000000"/>
+                            <param name="database" value="NCBI" />
+                        </conditional>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="all_datatypes/all_datatypes_trackhub.html">
+                <!-- verify tracks folder contains all the files -->
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam"
+                             value="all_datatypes/myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam"
+                             compare="sim_size"
+                />
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam.bai"
+                             value="all_datatypes/myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam.bai"
+                             compare="sim_size"
+                />
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/TBLASTN_Alignment_to_proteins.bb"
+                             value="all_datatypes/myHub/Dbia3/tracks/TBLASTN_Alignment_to_proteins.bb"
+                             compare="sim_size"
+                />
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/Repeating_Elements_by_TrfBig.bb"
+                             value="all_datatypes/myHub/Dbia3/tracks/Repeating_Elements_by_TrfBig.bb"
+                             compare="sim_size"
+                />
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/blastXmlToPsl.bb"
+                             value="all_datatypes/myHub/Dbia3/tracks/blastXmlToPsl.bb"
+                             compare="sim_size"
+                />
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/RNA-Seq_Alignment_Coverage.bigwig"
+                             value="all_datatypes/myHub/Dbia3/tracks/RNA-Seq_Alignment_Coverage.bigwig"
+                             compare="sim_size"
+                />
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/Augustus_Gene_Predictions.bb"
+                             value="all_datatypes/myHub/Dbia3/tracks/Augustus_Gene_Predictions.bb"
+                             compare="sim_size"
+                />
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb"
+                             value="all_datatypes/myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb"
+                             compare="sim_size"
+                />
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/Cytoband.bb"
+                             value="all_datatypes/myHub/Dbia3/tracks/Cytoband.bb"
+                             compare="sim_size"
+                />
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/regtools_junctions.bb"
+                             value="all_datatypes/myHub/Dbia3/tracks/regtools_junctions.bb"
+                             compare="sim_size"
+                />
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/BLAST_alignment_bigpsl.bb"
+                             value="all_datatypes/myHub/Dbia3/tracks/BLAST_alignment_bigpsl.bb"
+                             compare="sim_size"
+                />
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/BLAT_alignment_bigpsl.bb"
+                             value="all_datatypes/myHub/Dbia3/tracks/BLAT_alignment_bigpsl.bb"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="all_datatypes" />
+            </output>
+        </test>
+
+         <!-- Test with two groups and no tracks -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+            </repeat>
+            <repeat name="group">
+                <param name="group_name" value="Other group"/>
+            </repeat>
+            <output name="output" file="two_groups_no_track/two_groups_no_track_trackhub.html">
+                <expand macro="verify_hub_structure_no_track" test="two_groups_no_track" />
+            </output>
+        </test>
+
+        <!-- Test with two groups and one track in first -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bigwig"/>
+                        <param name="BIGWIG" value="bigwig/inputs/RNA-Seq_Alignment_Coverage"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <repeat name="group">
+                <param name="group_name" value="Other group"/>
+            </repeat>
+            <output name="output" file="two_groups_one_track_first/two_groups_one_track_first_trackhub.html">
+                <!-- Check tracks exist -->
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/RNA-Seq_Alignment_Coverage.bigwig"
+                             value="bigwig/myHub/Dbia3/tracks/RNA-Seq_Alignment_Coverage.bigwig"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="two_groups_one_track_first" />
+            </output>
+        </test>
+
+        <!-- Test with two groups and one track in both -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bigwig"/>
+                        <param name="BIGWIG" value="bigwig/inputs/RNA-Seq_Alignment_Coverage"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <repeat name="group">
+                <param name="group_name" value="Other group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="gtf"/>
+                        <param name="GTF" value="gtf/inputs/StringTie_Assembled_Transcripts"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="two_groups_one_track_both/two_groups_one_track_both_trackhub.html">
+                <!-- Check tracks exist -->
+                <!-- First group -->
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/RNA-Seq_Alignment_Coverage.bigwig"
+                             value="bigwig/myHub/Dbia3/tracks/RNA-Seq_Alignment_Coverage.bigwig"
+                             compare="sim_size"
+                />
+
+                <!-- Second group -->
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb"
+                             value="gtf/myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="two_groups_one_track_both" />
+                <!-- Check the groups.txt exists and is properly populated -->
+            </output>
+        </test>
+
+        <!-- Test with two groups and multiple tracks in both -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bigwig"/>
+                        <param name="BIGWIG" value="bigwig/inputs/RNA-Seq_Alignment_Coverage"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bam"/>
+                        <param name="BAM" value="bam/inputs/HISAT2_Accepted_Hits.bam"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <repeat name="group">
+                <param name="group_name" value="Other group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="gtf"/>
+                        <param name="GTF" value="gtf/inputs/StringTie_Assembled_Transcripts"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="gff3"/>
+                        <param name="GFF3" value="gff3_multi_fasta/inputs/Multi-Fasta_GlimmerHMM_Gene_Predictions"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="two_groups_multiple_tracks/two_groups_multiple_tracks_trackhub.html">
+                <!-- Check tracks exist -->
+                <!-- First group -->
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/RNA-Seq_Alignment_Coverage.bigwig"
+                             value="bigwig/myHub/Dbia3/tracks/RNA-Seq_Alignment_Coverage.bigwig"
+                             compare="sim_size"
+                />
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam"
+                             value="bam/myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam"
+                             compare="sim_size"
+                />
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam.bai"
+                             value="bam/myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam.bai"
+                             compare="sim_size"
+                />
+
+                <!-- Second group -->
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb"
+                             value="gtf/myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb"
+                             compare="sim_size"
+                />
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/Multi-Fasta_GlimmerHMM_Gene_Predictions.bb"
+                             value="gff3_multi_fasta/myHub/Dbia3/tracks/Multi-Fasta_GlimmerHMM_Gene_Predictions.bb"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="two_groups_multiple_tracks" />
+            </output>
+        </test>
+
+        <!-- Test default color in a track -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bam"/>
+                        <param name="BAM" value="bam/inputs/HISAT2_Accepted_Hits.bam"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="default_color/default_color_trackhub.html">
+                <expand macro="verify_hub_structure" test="default_color" />
+                <!-- Verify trackDb.txt contains the color 0,0,0 -->
+                <extra_files type="file" name="myHub/Dbia3/trackDb.txt">
+                    <assert_contents>
+                        <has_text text="color 0,0,0"/>
+                    </assert_contents>
+                </extra_files>
+
+                <!-- TODO: Find a way to check also that the whole common structure is intact too, without too much repetition -->
+            </output>
+        </test>
+
+        <!-- Test changed color in a track -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bam"/>
+                        <param name="BAM" value="bam/inputs/HISAT2_Accepted_Hits.bam"/>
+                        <param name="track_color" value="#8064a2"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="changed_color/changed_color_trackhub.html">
+                <expand macro="verify_hub_structure" test="changed_color" />
+                <!-- Verify trackDb.txt contains the color 128,100,162 -->
+                <extra_files type="file" name="myHub/Dbia3/trackDb.txt">
+                    <assert_contents>
+                        <has_text text="color 128,100,162"/>
+                    </assert_contents>
+                </extra_files>
+
+                <!-- TODO: Find a way to check also that the whole common structure is intact too, without too much repetition -->
+            </output>
+        </test>
+
+        <!-- Test for StringTie Chromosome end coordinates -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="gtf"/>
+                        <param name="GTF" ftype="gtf" value="stringtie_chromosome_end_coordinates/inputs/StringTie_Assembled_Transcripts"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="stringtie_chromosome_end_coordinates/stringtie_chromosome_end_coordinates_trackhub.html">
+                <extra_files type="file"
+                             name="myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb"
+                             value="stringtie_chromosome_end_coordinates/myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb"
+                             compare="sim_size"
+                />
+                <expand macro="verify_hub_structure" test="stringtie_chromosome_end_coordinates" />
+
+                <!-- TODO: Find a way to check also that the whole common structure is intact too, without too much repetition -->
+            </output>
+        </test>
+
+        <!-- Test default label in a track -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bam"/>
+                        <param name="BAM" value="bam/inputs/HISAT2_Accepted_Hits.bam"/>
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="default_color/default_color_trackhub.html">
+                <expand macro="verify_hub_structure" test="default_color" />
+                <!-- Verify trackDb.txt contains default label -->
+                <extra_files type="file" name="myHub/Dbia3/trackDb.txt">
+                    <assert_contents>
+                        <has_text text="longLabel HISAT2 Accepted Hits.bam"/>
+                    </assert_contents>
+                </extra_files>
+
+                <!-- TODO: Find a way to check also that the whole common structure is intact too, without too much repetition -->
+            </output>
+        </test>
+
+        <!-- Test customized label in a track -->
+        <test>
+            <param name="genome_name" value="Dbia3"/>
+            <param name="fasta_file" value="common/dbia3.fa"/>
+            <repeat name="group">
+                <param name="group_name" value="Default group"/>
+                <repeat name="format">
+                    <conditional name="formatChoice">
+                        <param name="format_select" value="bam"/>
+                        <param name="BAM" value="bam/inputs/HISAT2_Accepted_Hits.bam"/>
+                        <param name="longLabel" value="HISAT sequence alignment" />
+                        <param name="track_color" value="#000000"/>
+                    </conditional>
+                </repeat>
+            </repeat>
+            <output name="output" file="changed_label/changed_label_trackhub.html">
+                <expand macro="verify_hub_structure" test="changed_label" />
+                <!-- Verify trackDb.txt contains the customized label -->
+                <extra_files type="file" name="myHub/Dbia3/trackDb.txt">
+                    <assert_contents>
+                        <has_text text="longLabel HISAT sequence alignment"/>
+                    </assert_contents>
+                </extra_files>
+
+                <!-- TODO: Find a way to check also that the whole common structure is intact too, without too much repetition -->
+            </output>
+        </test>
+
+        
+
+
+        <!-- Test for big files? -->
+
+        <!-- Find tests that should fail -->
+
+    </tests>
+
+    <help>
+        This Galaxy tool permits to prepare your files to be ready for
+        Assembly Hub visualization.
+    </help>
+
+    <citations>
+        <citation type="doi">10.7490/f1000research.1112719.1</citation>
+    </citations>
+</tool>