# HG changeset patch
# User trinity_ctat
# Date 1504208214 14400
# Node ID 4c1d32673942bdac4d4b5a15da09edc7f571254f
# Parent  f976a93748a401822981277a78ffa5396f33f186
Uploaded

diff -r f976a93748a4 -r 4c1d32673942 trinity.xml
--- a/trinity.xml	Tue Aug 29 12:04:32 2017 -0400
+++ b/trinity.xml	Thu Aug 31 15:36:54 2017 -0400
@@ -1,17 +1,22 @@
 <tool id="trinityrnaseq" name="Trinity" version="2.4.0">
   
-    <!-- Originally written by Jeremy Goecks,
-        later maintained by (in chronological order)
+    <!-- Original written by Jeremy Goecks,
+        later development/maintenance by (in chronological order)
             bhaas, Ben Fulton, Cicada Dennis 
     -->
     <description>De novo assembly of RNA-Seq data using Trinity 2.4.0</description>
     <requirements>
+        <requirement type="package" version="2.7">python</requirement>
+        <requirement type="package">subprocess32</requirement>
+        <requirement type="package">samtools</requirement>
+        <requirement type="package">bzip2</requirement>
         <requirement type="package" version="2.4.0">trinity</requirement>
     </requirements>
     <command>
       <![CDATA[
-      python $__tool_directory__/trinity_wrapper.py --mem_per_cpu 31
+      python $__tool_directory__/trinity_wrapper.py 
       --CPU \${GALAXY_SLOTS:-4}
+      --max_memory \${TRINITY_MAX_MEMORY:-31G}
       #if str($inputs.paired_or_single) == "paired":
        --left $inputs.left_input --right $inputs.right_input
        #if $inputs.left_input.ext == 'fasta':
@@ -29,12 +34,17 @@
       #end if
       ## direct to output
       --timing trinity_out_dir/Trinity.timing
-      --user $__user_id__
-      --fullpath /N/dc2/scratch/tstrnity/rerun
-      --dir '$adv.rerundir'
       --log $trinity_log
 
  ]]>
+      <!-- The fullpath, dir, and user options (preceed with dashes) can be
+           used in the trinity_wrapper.py command to create rerunable jobs.
+           We are not supporting rerunable jobs in this release of this tool.
+        fullpath \${TRINITY_RERUN_PREFIX}
+        dir '$adv.rerundir'
+        user $__user_id__
+      mem_per_cpu 31
+      -->
     </command>
     <stdio>
       <exit_code range="1:"   level="fatal"   description="Program failed" />
@@ -54,9 +64,12 @@
           <param format="fasta,fastq" name="input" type="data" label="Single-end reads" help=""/>
         </when>
       </conditional>
+      <!-- The following section was used to aid in creating rerunable jobs.
+           We are not supporting rerunable jobs in this release of this tool.
       <section name="adv" title="Allow Job Rerun" expanded="False">
-	<param name="rerundir" type="text" size="10" label="To make a job rerunnable, you will need to specify a unique tag to label the job, with no spaces or wierd characters." />
-    </section>
+        <param name="rerundir" type="text" size="10" label="To make a job rerunnable, you will need to specify a unique tag to label the job, with no spaces or wierd characters." />
+      </section>
+      -->
     </inputs>
     <outputs>
       <data format="txt" name="trinity_log" label="${tool.name} on ${on_string}: log" />
@@ -90,16 +103,13 @@
             <param name="left_input" value="Sp.cat_ds_hs.left.fq" />
             <param name="right_input" value="Sp.cat_ds_hs.right.fq" />
             <param name="adv.rerundir" value="planemo_test_2" />
-            <!-- Following are not being used in this version of trinity.xml -->
+            <!-- Following parameters are not used in this version of this tool. -->
             <!--
- 	    <param name="paired_or_single" value="paired" />
-            <param name="left_input" file="cat_Sp.left.fq" />
-            <param name="right_input" file="cat_Sp.right.fq" />
             <param name="JM" value="50G" />
             <param name="CPU" value="2" />
             <param name="library_type" value="None" />
             <param name="group_pairs_distance" value="500" />
-            <param name="path_reinforcement_distance" va;ue="75" />
+            <param name="path_reinforcement_distance" value="75" />
             <param name="use_additional" value="no" />
             -->
   	    <output name="trinity_log" >
@@ -119,7 +129,8 @@
     <help>
 This instance is running Trinity version 2.4.0 and uses the following command:
 
-	 Trinity --max_memory 240G --CPU 8 --seqType seq_type --single singlefile or --left left_file --right right_file
+	 Trinity --max_memory \${TRINITY_MAX_MEMORY:-31G} --CPU \${GALAXY_SLOTS:-4} --seqType seq_type --single singlefile or --left left_file --right right_file
+         Define TRINITY_MAX_MEMORY and GALAXY_SLOTS to change the default values for --max_memory and --CPU.
 
 .. class:: infomark