# HG changeset patch # User trinity_ctat # Date 1513365509 18000 # Node ID f90885dda34c86577012b63e334978e2f81e8f74 # Parent 0c6f8f4e77336eea88df434e2b43d6ca860169fa Adding star_fusion tool. diff -r 0c6f8f4e7733 -r f90885dda34c star_fusion.xml --- a/star_fusion.xml Fri Dec 15 14:12:30 2017 -0500 +++ b/star_fusion.xml Fri Dec 15 14:18:29 2017 -0500 @@ -1,14 +1,16 @@ - + Fusion-finding Pipeline Using the STAR Aligner star-fusion - STAR-Fusion - --genome_lib_dir "${genome_ref_lib.fields.path}" - --left_fq "${left_input} - --right_fq "${right_input} - --output_dir subdir + diff -r 0c6f8f4e7733 -r f90885dda34c trinity.xml --- a/trinity.xml Fri Dec 15 14:12:30 2017 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,148 +0,0 @@ - - - - De novo assembly of RNA-Seq data using Trinity 2.4.0 - - python - subprocess32 - samtools - bzip2 - trinity - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -This instance is running Trinity version 2.4.0 and uses the following command: - - Trinity --max_memory 31G --CPU 4 --seqType seq_type --single singlefile or --left left_file --right right_file - -Define TRINITY_MAX_MEMORY and GALAXY_SLOTS to change the default values for --max_memory and --CPU, respectively. - -.. class:: infomark - -Trinity_, developed at the Broad Institute and the Hebrew University of Jerusalem, represents a novel method for the efficient and robust de novo reconstruction of transcriptomes from RNA-seq data. Trinity combines three independent software modules: Inchworm, Chrysalis, and Butterfly, applied sequentially to process large volumes of RNA-seq reads. Trinity partitions the sequence data into many individual de Bruijn graphs, each representing the transcriptional complexity at a given gene or locus, and then processes each graph independently to extract full-length splicing isoforms and to tease apart transcripts derived from paralogous genes. For more information, visit Trinity's wiki page here_. - -.. _Trinity: https://github.com/trinityrnaseq/trinityrnaseq/wiki -.. _here: https://github.com/trinityrnaseq/trinityrnaseq/wiki - - - - 10.1038/nbt.1883 - - -