Mercurial > repos > tduigou > sculpt_sequences
annotate sculpt_sequences.xml @ 1:a0cd867780ec draft default tip
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
| author | tduigou |
|---|---|
| date | Thu, 17 Jul 2025 13:24:49 +0000 |
| parents | 0c7f75a2338b |
| children |
| rev | line source |
|---|---|
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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1 <tool id="sculpt_sequences" name="Sculpt Sequences" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="21.09"> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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2 <description>Optimize DNA sequences</description> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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3 <macros> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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4 <token name="@VERSION_SUFFIX@">1</token> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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5 <token name="@TOOL_VERSION@">0.2.0</token> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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6 </macros> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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7 <requirements> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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8 <requirement type="package" version="0.1.11">flametree</requirement> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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9 <requirement type="package" version="1.85"> biopython </requirement> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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10 <requirement type="package" version="0.1.10">proglog</requirement> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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11 <requirement type="package" version="3.2.16">dnachisel</requirement> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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12 <requirement type="package" version="2025.4.15">html2text</requirement> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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13 <requirement type="package" version="2.0.12">dnacauldron</requirement> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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14 <requirement type="package" version="2.2.3">pandas</requirement> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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15 <requirement type="package" version="2.2.5">numpy</requirement> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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16 <requirement type="package" version="0.3.9">pdf-reports</requirement> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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17 <requirement type="package" version="0.1.8">sequenticon</requirement> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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18 <requirement type="package" version="3.1.5">dna_features_viewer</requirement> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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19 </requirements> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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20 <command detect_errors="exit_code"><![CDATA[ |
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1
a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
tduigou
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21 #if str($json_use.use_json_param) == "false": |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
tduigou
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22 #set avoid_list = [line.strip() for line in str($avoid_patterns).strip().split('\n') if line.strip()] |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
tduigou
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23 #set avoid_patterns = ','.join($avoid_list) |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
tduigou
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24 |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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25 #set hairpin_lines = [line.strip() for line in str($json_use.hairpin_constraints).strip().split('\n') if line.strip()] |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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26 #set hairpin_constraints = '__cn__'.join($hairpin_lines) |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
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27 |
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1
a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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28 #set gc_lines = [line.strip() for line in str($json_use.gc_constraints).strip().split('\n') if line.strip()] |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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29 #set gc_constraints = '__cn__'.join($gc_lines) |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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30 |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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31 #set kmer_size = $json_use.kmer_size |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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32 |
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1
a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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33 #else: |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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34 #set avoid_patterns = '' |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
tduigou
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35 #set hairpin_constraints = '' |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
tduigou
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36 #set gc_constraints = '' |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
tduigou
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37 #set kmer_size = '' |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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38 #end if |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
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39 |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
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40 #set genbank_file_paths = ','.join([str(f) for f in $genbank_files]) |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
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41 #set $file_name_mapping = ",".join(["%s:%s" % (file.file_name, file.name) for file in $genbank_files]) |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
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42 |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
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43 mkdir 'outdir_scul' && |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
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44 mkdir 'outdir_unscul' && |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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45 python '$__tool_directory__/sculpt_sequences.py' |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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46 --use_json_param '$json_use.use_json_param' |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
tduigou
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47 --files_to_sculpt '$genbank_file_paths' |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
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48 --file_name_mapping '$file_name_mapping' |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
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49 --outdir_scul 'outdir_scul' |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
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50 --outdir_unscul 'outdir_unscul' |
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1
a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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51 --use_file_names_as_id '$use_file_names_as_ids' |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
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52 --avoid_patterns '$avoid_patterns' |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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53 --gc_constraints '$gc_constraints' |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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54 --kmer_size '$kmer_size' |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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55 --hairpin_constraints '$hairpin_constraints' |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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56 #if $json_use.use_json_param: |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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57 --json_params '$json_use.json_params' |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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58 #else: |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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59 --json_params '' |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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60 --DnaOptimizationProblemClass '$json_use.DnaOptimizationProblemClass' |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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61 #end if |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
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62 ]]></command> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
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63 <inputs> |
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0c7f75a2338b
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit fa4c85dd6ad48d404a28e21667f18b628bbdc702-dirty
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64 <param name="genbank_files" type="data_collection" collection_type="list" format="genbank" label="GenBank File(s)"/> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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65 <conditional name='json_use'> |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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66 <param name="use_json_param" type="boolean" checked="false" label="Use parameter from a JSON file" /> |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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67 <when value="false"> |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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68 <param name="DnaOptimizationProblemClass" type="select" label="DnaOptimizationProblem Calss" help="select the assambly class"> |
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a0cd867780ec
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6b6ce806b5d016b3c7f20318180eff2dbe64395a-dirty
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69 <option value="DnaOptimizationProblem" selected="True">DnaOptimizationProblem</option> |
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70 <option value="CircularDnaOptimizationProblem">CircularDnaOptimizationProblem</option> |
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71 </param> |
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72 <param name="avoid_patterns" type="text" area="true" label="Avoid Pattern Constraints" help="Each pattern on a line" /> |
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73 <param name="hairpin_constraints" type="text" area="true" label="Hairpins Constraints" optional="true" help="e.g. (you can add others Hairpins Constraints on a new line): stem_size=20, hairpin_window=200"/> |
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74 <param name="gc_constraints" type="text" area="true" label="Enforce GC Content Constraints" optional="true" help="e.g. (you can add others Enforce GC Content Constraints on a new line): mini=0.3, maxi=0.7, window=100"/> |
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75 <param name="kmer_size" type="integer" label="K-mer Uniqueness Size" value="" optional="true" help="e.g.: 15"/> |
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76 </when> |
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77 <when value="true"> |
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78 <param name="json_params" type="data" format="json" optional="true" label="JSON parameters file" help="Contains tool's parameters" /> |
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79 </when> |
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80 </conditional> |
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81 <param name="use_file_names_as_ids" type="boolean" checked="True" label="Use File Names As Sequence IDs" /> |
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82 </inputs> |
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83 <outputs> |
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84 <collection name="scul" type="list" label="scul group" > |
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85 <discover_datasets pattern="(?P<name>.*).zip" format="zip" directory="outdir_scul" /> |
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86 </collection> |
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87 <collection name="unscul" type="list" label="unscul+scul gb" > |
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88 <discover_datasets pattern="(?P<name>.*).gb" format="genbank" directory="outdir_unscul" /> |
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89 </collection> |
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90 </outputs> |
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91 <tests> |
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92 <test> |
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93 <!-- test for DnaOptimizationProblem --> |
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94 <param name="genbank_files"> |
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95 <collection type="list"> |
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96 <element name="p15_PuroR" value="10_emma_genbanks/p15_PuroR.gb" /> |
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97 <element name="p9_PuroR" value="10_emma_genbanks/p9_PuroR.gb" /> |
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98 <element name="p15_Pup9_mTagBFP2roR" value="10_emma_genbanks/p9_mTagBFP2.gb" /> |
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99 <element name="p15_p9_BSDRPuroR" value="10_emma_genbanks/p9_BSDR.gb" /> |
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100 <element name="p8_Linker1" value="10_emma_genbanks/p8_Linker1.gb" /> |
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101 <element name="p7_L7Ae-Weiss" value="10_emma_genbanks/p7_L7Ae-Weiss.gb" /> |
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102 <element name="p6_Nt-IgKLsequence" value="10_emma_genbanks/p6_Nt-IgKLsequence.gb" /> |
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103 <element name="p6_Kozak-ATG" value="10_emma_genbanks/p6_Kozak-ATG.gb" /> |
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104 <element name="p4_Kt-L7Ae-Weiss" value="10_emma_genbanks/p4_Kt-L7Ae-Weiss.gb" /> |
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105 <element name="HC_Amp_ccdB" value="10_emma_genbanks/HC_Amp_ccdB.gb" /> |
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106 </collection> |
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107 </param> |
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1
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108 <conditional name="json_use"> |
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109 <param name="use_json_param" value="false" /> |
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110 <param name="DnaOptimizationProblemClass" value="DnaOptimizationProblem" /> |
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111 <!-- AvoidPatterns --> |
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112 <param name="avoid_patterns" value="BsaI_site |
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113 NotI_site |
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114 XbaI_site |
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115 ClaI_site |
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116 8x1mer" /> |
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117 <!-- EnforceGCContent --> |
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118 <param name="gc_constraints" value="mini=0.1, maxi=0.9, window=50"/> |
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119 </conditional> |
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120 <param name="use_file_names_as_ids" value="True" /> |
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121 <output_collection name="scul" count="10"> |
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122 </output_collection> |
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123 <output_collection name="unscul" count="10"> |
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124 </output_collection> |
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125 </test> |
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126 <test> |
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127 <!-- test for CircularDnaOptimizationProblem --> |
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128 <param name="genbank_files"> |
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129 <collection type="list"> |
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130 <element name="p15_PuroR" value="10_emma_genbanks/p15_PuroR.gb" /> |
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131 <element name="p9_PuroR" value="10_emma_genbanks/p9_PuroR.gb" /> |
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132 <element name="p15_Pup9_mTagBFP2roR" value="10_emma_genbanks/p9_mTagBFP2.gb" /> |
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133 <element name="p15_p9_BSDRPuroR" value="10_emma_genbanks/p9_BSDR.gb" /> |
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134 <element name="p8_Linker1" value="10_emma_genbanks/p8_Linker1.gb" /> |
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135 <element name="p7_L7Ae-Weiss" value="10_emma_genbanks/p7_L7Ae-Weiss.gb" /> |
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136 <element name="p6_Nt-IgKLsequence" value="10_emma_genbanks/p6_Nt-IgKLsequence.gb" /> |
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137 <element name="p6_Kozak-ATG" value="10_emma_genbanks/p6_Kozak-ATG.gb" /> |
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138 <element name="p4_Kt-L7Ae-Weiss" value="10_emma_genbanks/p4_Kt-L7Ae-Weiss.gb" /> |
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139 <element name="HC_Amp_ccdB" value="10_emma_genbanks/HC_Amp_ccdB.gb" /> |
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140 </collection> |
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141 </param> |
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142 <conditional name="json_use"> |
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143 <param name="use_json_param" value="false" /> |
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144 <param name="DnaOptimizationProblemClass" value="CircularDnaOptimizationProblem" /> |
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145 <!-- AvoidPatterns --> |
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146 <param name="avoid_patterns" value="BsaI_site |
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147 NotI_site |
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148 XbaI_site |
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149 ClaI_site |
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150 8x1mer" /> |
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151 <!-- EnforceGCContent --> |
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152 <param name="gc_constraints" value="mini=0.1, maxi=0.9 |
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153 mini=0.3, maxi=0.7, window=50"/> |
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154 <param name="hairpin_constraints" value="stem_size=20, hairpin_window=200 |
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155 stem_size=10, hairpin_window=100"/> |
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156 </conditional> |
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157 <param name="use_file_names_as_ids" value="True" /> |
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158 <output_collection name="scul" count="10"> |
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159 </output_collection> |
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160 <output_collection name="unscul" count="10"> |
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161 </output_collection> |
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162 </test> |
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163 <test> |
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164 <!-- test json params --> |
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165 <param name="genbank_files"> |
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166 <collection type="list"> |
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167 <element name="p15_PuroR" value="10_emma_genbanks/p15_PuroR.gb" /> |
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168 <element name="p9_PuroR" value="10_emma_genbanks/p9_PuroR.gb" /> |
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169 <element name="p15_Pup9_mTagBFP2roR" value="10_emma_genbanks/p9_mTagBFP2.gb" /> |
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170 <element name="p15_p9_BSDRPuroR" value="10_emma_genbanks/p9_BSDR.gb" /> |
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171 <element name="p8_Linker1" value="10_emma_genbanks/p8_Linker1.gb" /> |
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172 <element name="p7_L7Ae-Weiss" value="10_emma_genbanks/p7_L7Ae-Weiss.gb" /> |
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173 <element name="p6_Nt-IgKLsequence" value="10_emma_genbanks/p6_Nt-IgKLsequence.gb" /> |
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174 <element name="p6_Kozak-ATG" value="10_emma_genbanks/p6_Kozak-ATG.gb" /> |
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175 <element name="p4_Kt-L7Ae-Weiss" value="10_emma_genbanks/p4_Kt-L7Ae-Weiss.gb" /> |
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176 <element name="HC_Amp_ccdB" value="10_emma_genbanks/HC_Amp_ccdB.gb" /> |
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177 </collection> |
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178 </param> |
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179 <conditional name="json_use"> |
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180 <param name="use_json_param" value="true" /> |
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181 <param name="json_params" value="test_json_workflow2.json" /> |
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182 </conditional> |
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183 <param name="use_file_names_as_ids" value="True" /> |
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184 <output_collection name="scul" count="10"> |
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185 </output_collection> |
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186 <output_collection name="unscul" count="10"> |
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187 </output_collection> |
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188 </test> |
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189 </tests> |
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190 <help><![CDATA[ |
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191 Sculpt Sequences |
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192 ================= |
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193 |
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194 Sculpt Sequences is a Python library from the EGF Biofoundry for problem detection and sequence optimization using `dnachisel <https://github.com/Edinburgh-Genome-Foundry/DnaChisel/tree/master/dnachisel>`_ (Complete documentation available `here <https://edinburgh-genome-foundry.github.io/DnaChisel/>`_) |
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195 |
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196 **Parameters**: |
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197 --------------- |
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198 * **GenBank File(s)**: List of GenBank files to be processed. |
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199 * **Use parameter from a JSON file**: |
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200 Yes/No parameter to indicate if user want to set parameter manually or using a json file |
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201 If Yes, user should provide a JSON file contains all parameters |
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202 * **DnaOptimizationProblem Class**: |
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203 - "DnaOptimizationProblem": is the class to define and solve an optimization problems. Its methods implement all the solver logics. |
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204 - "CircularDnaOptimizationProblem": is a variant of DnaOptimizationProblem whose optimization algorithm assumes that the sequence is circular. |
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205 * **Avoid Pattern Constraints**: is a sequence design rules that can be used as constraints. It define pattern(s) to avoid during problem optimisation. |
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206 This can include enzyme sites like "BsaI_site", "NotI_site", "XbaI_site"... `enzyme dict <https://github.com/biopython/biopython/blob/master/Bio/Restriction/Restriction_Dictionary.py>`_ . Custom patterns are also supported, such as "5x3mer" means "any 5 consecutive 3-nucleotide sequences — typically 5 unique 3-mers in a row. |
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207 * **Enforce GC Content Constraints**: |
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208 Define acceptable GC content ranges. For example min: 0.4, max: 0.6, window: 50 represents a 40–60% GC content requirement within a 50-base window. |
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209 (Parameters: `EnforceGCContent_params <https://edinburgh-genome-foundry.github.io/DnaChisel/ref/builtin_specifications#enforcegccontent>`_ ) |
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210 * **Avoid Hairpins**: Avoid Hairpin patterns as defined by the IDT guidelines. |
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211 A hairpin is defined by a sequence segment which has a reverse complement “nearby” in a given window. |
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212 (Parameters: `AvoidHairpins_params <https://edinburgh-genome-foundry.github.io/DnaChisel/ref/builtin_specifications#avoidhairpins>`_ ). |
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213 * **K-mer Uniqueness Size**: Avoid sub-sequence of length k with homologies elsewhere. |
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214 * **Use File Names As Sequence IDs**: Recommended if the GenBank file names represent the fragment names. |
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215 ]]></help> |
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216 <citations> |
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217 <citation type="bibtex"> |
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218 @unpublished{sculpt_sequences |
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219 author = {Ramiz Khaled}, |
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220 title = {{sculpt_sequences}}, |
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221 url = {https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb}, |
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222 } |
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223 </citation> |
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224 </citations> |
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225 </tool> |
