# HG changeset patch # User tduigou # Date 1652798968 0 # Node ID a2b98a6e033b6353623f8cf89f60ab6c095031ba # Parent 9603ff31672610efd6c1ee61f98b070fb4780a6c "planemo upload commit f40274f6b9f6a15eb4022aab21286d4c96cd8475-dirty" diff -r 9603ff316726 -r a2b98a6e033b rpbasicdesign.xml --- a/rpbasicdesign.xml Fri May 06 16:25:16 2022 +0000 +++ b/rpbasicdesign.xml Tue May 17 14:49:28 2022 +0000 @@ -1,6 +1,7 @@ - + Build DNA-BOT input files from rpSBML + 0 1.1.0 @@ -129,18 +130,12 @@ `_, to generate genetic constructs compliant with the BASIC (Biopart Assembly Standard for Idempotent Cloning) assembly approach. CSV files produced are ready to be used with DNA-Bot to generate instructions for automated build of the genetic constructs using OpenTrons liquid handling robots. - Input ----- - Required: - * **rpSBML file**\ : rpSBML file from which enzymes UniProt IDs will be collected. - Advanced options: - * **Linkers and user parts**\ : (string) List of files providing available linkers and user parts (backbone, promoters, ...) for constructs. Default: ( `Standard Biolegio Parts `_: BBP-18500). * **LMS part ID**\ : (string) part ID to be used as the LMS methylated linker. Default: LMS. * **LMP part ID**\ : (string) part ID to be used as the LMP methylated linker. Default: LMP. @@ -149,25 +144,18 @@ * **Perform CDS permutation?**\ : (boolean) Whether all combinations of CDS permutation should be built Default: true. * **Maximum number of enyzme to consider per reaction**\ : (int) Maximum number of enyzme to consider per reaction. If more enzymes are available for a given reaction, then only the last one listed in the MIRIAM annotation section will be kept. (Default: 1). * **Maximum number of genes per construct**\ : (int) If more genes are required, i.e. more reactions are described in the input SBML file, then the execution will failed. (Default: 3). - Output ------ - * **constructs**\ : CSV construct file listing the constructs to be built. * **User parts plate**\ : CSV file listing the DNA parts to be included into each construct. * **Biolegio plate**\ : CSV file listing the plate coordinates of the BASIC linkers. * **SBOL constructs**\ : (optional) one SBOL (Synthetic Biology Open Language) file is produced for each construct generated in XML format. - Project Links ------------------ - * `GitHub `_ - License ------- - * `MIT `_ - ]]> 10.1093/synbio/ysaa010