Mercurial > repos > tduigou > domestication
diff domestication.xml @ 8:4bfdec0be6b1 draft
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Examples/blob/master/templates/template1.ipynb commit 6ae809b563b40bcdb6be2e74fe2a84ddad5484ae
| author | tduigou |
|---|---|
| date | Fri, 23 May 2025 09:04:43 +0000 |
| parents | 70895bec2486 |
| children | 450ed9dca5d5 |
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--- a/domestication.xml Mon May 19 14:04:56 2025 +0000 +++ b/domestication.xml Fri May 23 09:04:43 2025 +0000 @@ -1,4 +1,4 @@ -<tool id="domestication" name="domestication" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="21.09"> +<tool id="domestication" name="Domestication Of New Parts" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="21.09"> <description>Enables rapid and accurate preparation of DNA parts for modular cloning systems, such as Golden Gate Assembly</description> <macros> <token name="@VERSION_SUFFIX@">1</token> @@ -34,11 +34,11 @@ ]]></command> <inputs> <param name="genbank_files" type="data_collection" collection_type="list" format="genbank" label="GenBank File(s)"/> - <param name="domestication_csv" type="data" format="csv" label="deomestication csv"/> + <param name="domestication_csv" type="data" format="csv" label="Deomestication csv"/> <param name="methylation_protection" type="boolean" checked="False" label="Enabel Methylation Protection"/> <section name="adv" title="Advanced Options" expanded="false"> - <param name="use_file_names_as_ids" type="boolean" checked="True" label="Use file names as sequence IDs?" /> - <param name="allow_edits" type="boolean" checked="True" label="Allow sequence edits" help="If False, sequences cannot be edited by the domesticator, only extended + <param name="use_file_names_as_ids" type="boolean" checked="True" label="Use File Names As Sequence IDs" /> + <param name="allow_edits" type="boolean" checked="True" label="Allow Sequence Edits" help="If False, sequences cannot be edited by the domesticator, only extended with flanks. If a sequence has for instance forbidden restriction sites, the domesticaton will fail for this sequence (and this will be noted in the report."/> @@ -184,19 +184,18 @@ </test> </tests> <help><![CDATA[ -Domestication -================= +Domestication Of New Parts +========================== -The Domestication Tool in the EGF Biofoundry, powered by Genedom, enables rapid and accurate preparation of DNA parts for modular cloning systems, such as Golden Gate Assembly (complete documentation `here <https://edinburgh-genome-foundry.github.io/genedom/>`_) -This tool streamlines the domestication process by identifying and removing internal restriction site like "BsaI_site", "NotI_site", "XbaI_site"...(`enzyme dict <https://github.com/biopython/biopython/blob/master/Bio/Restriction/Restriction_Dictionary.py>`_ ) or costumised like "5x3mer" means "any 5 consecutive 3-nucleotide sequences — typically 5 unique 3-mers in a row. +The Domestication tool from the EGF Biofoundry, powered by Genedom, enables rapid and accurate add of the flanking enzyme sites to each DNA part preparing it for modular cloning systems, such as Golden Gate Assembly (complete documentation `here <https://edinburgh-genome-foundry.github.io/genedom/>`_) **Parameters**: --------------- * **GenBank File(s)**: GenBank files for domestication (can be output of sculpt tool) -* **deomestication csv**: csv file containes the construct names with their parts.`example <https://cuba.genomefoundry.org/static/file_examples/domesticate_part_batches/EMMA.csv>`_ +* **Deomestication csv**: csv file containes the construct names with their parts.`example <https://cuba.genomefoundry.org/static/file_examples/domesticate_part_batches/EMMA.csv>`_ * **Enabel Methylation Protection**: Enable the methylation protection. -* **Allow sequence edits**: Recommanded. If False, sequences cannot be edited by the domesticator, only extended with flanks. If a sequence has for instance forbidden restriction sites, the domesticaton will fail for this sequence (and this will be noted in the report. -* **Use file names as sequence IDs?**: Recommended if the GenBank file names represent the fragment names. +* **Allow Sequence Edits**: Recommanded. If False, sequences cannot be edited by the domesticator, only extended with flanks. If a sequence has for instance forbidden restriction sites, the domesticaton will fail for this sequence (and this will be noted in the report. +* **Use File Names As Sequence IDs**: Recommended if the GenBank file names represent the fragment names. * **output**: Zip report for domestication process + domesticated gb files (methylation pretected in case of enable of methylation protecton option). ]]></help> <citations>
