LOCUS       .                       2529 bp    DNA              UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    complement(6..11)
                     /note="BsmBI"
     terminator      86..115
                     /note="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     rep_origin      137..724
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      736..763
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     source          5..1150
                     /source="Exported"
     promoter        complement(1603..1705)
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene"
     terminator      1706..1737
                     /note="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     misc_feature    1803..1808
                     /note="BsmBI"
     source          1155..1809
                     /source="Exported"
     CDS             1816..2523
                     /codon_start=1
                     /note="mRuby2"
                     /product="monomeric red fluorescent protein derived from
                     mRuby, with improved photophysical properties (Lam et al.,
                     2012)"
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     source          1814..2529
                     /source="Exported"
ORIGIN
        1 tgctcgagac gaaaacgaat tcaagcttga tatcattcag gacgagcctc agactccagc
       61 gtaactggac tgcaatcaac tcactggctc accttcacgg gtgggccttt cttcggtaga
      121 aaatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      181 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      241 cgaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
      301 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
      361 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
      421 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      481 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      541 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      601 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      661 tcgccacctc tgacttgagc atcgattttt gtgatgctcg tcaggggggc ggagcctatg
      721 gaaaaacgcc agcaacgcag aaaggcccac ccgaaggtga gccaggtgat tacatttggg
      781 ccctcattag aaaaactcat cgagcatcaa gtgaaactgc aatttattca tatcaggatt
      841 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
      901 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
      961 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
     1021 gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac
     1081 aggccagcca ttacgctcgt catcaaaatc actcgcacca accaaaccgt tattcattcg
     1141 tgattgcgcc tgagcgagac gaaatacgcg atcgccgtta aaaggacaat tacaaacagg
     1201 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
     1261 aggatattct tctaatacct ggaatgctgt tttccctggg atcgcagtgg tgagtaacca
     1321 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
     1381 ccagtttagc ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     1441 cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg
     1501 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     1561 tcgcggcctc gagcaagacg tttcccgttg aatatggctc attttagctt ccttagctcc
     1621 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     1681 gttggaacct cttacgtgcc gatcaagtca aaagcctccg gtcggaggct tttgactttc
     1741 tgctatggag gtcaggtatg atttaaatgg tcagtattga gcgatatcta gagaattcgt
     1801 cacgtctcgg cgtccgtgtc taagggcgaa gagctgatca aggaaaatat gcgtatgaag
     1861 gtggtcatgg aaggttcggt caacggccac caattcaaat gcacaggtga aggagaaggc
     1921 aatccgtaca tgggaactca aaccatgagg atcaaagtca tcgagggagg acccctgcca
     1981 tttgcctttg acattcttgc cacgtcgttc atgtatggca gccgtacttt tatcaagtac
     2041 ccgaaaggca ttcctgattt ctttaaacag tcctttcctg agggttttac ttgggaaaga
     2101 gttacgagat acgaagatgg tggagtcgtc accgtcatgc aggacaccag ccttgaggat
     2161 ggctgtctcg tttaccacgt ccaagtcaga ggggtaaact ttccctccaa tggtcccgtg
     2221 atgcagaaga agaccaaggg ttgggagcct aatacagaga tgatgtatcc agcagatggt
     2281 ggtctgaggg gatacactca tatggcactg aaagttgatg gtggtggcca tctgtcttgc
     2341 tctttcgtaa caacttacag gtcaaaaaag accgtcggga acatcaagat gcccggtatc
     2401 catgccgttg atcaccgcct ggaaaggtta gaggaaagtg acaatgaaat gttcgtagta
     2461 caacgcgaac acgcagttgc caagttcgcc gggcttggtg gtgggatgga cgagctgtac
     2521 aagtaataa
//