Mercurial > repos > rnateam > veinnarna_rnaduplex

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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/vienna_rna commit d1bef7f37f272e3173c137fcb690364fee7e6d1f
author rnateam
date Wed, 05 Oct 2016 04:59:23 -0400
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278760bf4bb2 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/vienna_rna commit d1bef7f37f272e3173c137fcb690364fee7e6d1f
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1 <tool id="veinnarna_rnaduplex" name="@EXECUTABLE@" version="@VERSION@.0">
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2 <description>Compute the structure upon hybridization of two RNA strands</description>
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3 <macros>
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4 <token name="@EXECUTABLE@">RNAduplex</token>
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5 <import>macros.xml</import>
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6 </macros>
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7 <expand macro="requirements" />
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8 <expand macro="stdio" />
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9 <expand macro="version_command" />
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10 <command>
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11 <![CDATA[
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12 RNAduplex < '$input' > '$tabularFile'
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13 -T$temperature -d$dangling --deltaEnergy=$denergy
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14 #if $varExists('$advancedOptions.noconv')
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15 $advancedOptions.noconv
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16 $advancedOptions.noLP
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17 $advancedOptions.noGU
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18 $advancedOptions.noClosingGU
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19 $advancedOptions.noTetra
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20 #if $advancedOptions.nsp
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21 --nsp='$advancedOptions.nsp'
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22 #end if
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23 #end if
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24 ]]>
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25 </command>
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26 <inputs>
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27 <param format="fasta" name="input" type="data" label="Fasta file"/>
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28 <param name="temperature" type="float" value="37.0" label="temperature [°C]" help="-T"/>
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29 <param name="dangling" type="select" label="how to treat dangling end energies" help="-d">
278760bf4bb2 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/vienna_rna commit d1bef7f37f272e3173c137fcb690364fee7e6d1f
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30 <option value="0">0: ignore dangling ends</option>
278760bf4bb2 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/vienna_rna commit d1bef7f37f272e3173c137fcb690364fee7e6d1f
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31 <option value="1">1: unpaired bases participate in one dangling end only</option>
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32 <option value="2" selected="True">2: unpaired bases participate in all dangling ends</option>
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33 <option value="3">3: allow coaxial stacking</option>
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34 </param>
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35 <param argument="--denergy" type="float" value="0.0" label="delta energy range [kcal/mol] for suboptimal structures" help=""/>
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36 <param argument="--sorted" type="boolean" truevalue="--sorted" falsevalue="" checked="false" label="Sort by energy" help=""/>
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37 <conditional name="advancedOptions">
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38 <param name="advancedSelector" type="select" label="advanced options">
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39 <option value="basic">basic Options</option>
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40 <option value="advanced">advanced Options</option>
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41 </param>
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42 <when value="advanced">
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43 <param argument="--noconv" type="boolean" truevalue="--noconv" falsevalue="" checked="false" label="No converstion from T->U" help=""/>
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44 <param argument="--noLP" type="boolean" truevalue="--noLP" falsevalue="" checked="false" label="No lonely pairs" help="don't allow lonely pairs."/>
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45 <param argument="--noGU" type="boolean" truevalue="--noGU" falsevalue="" checked="false" label="No GU pairing" help="don't allow pairing of G and U."/>
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46 <param argument="--noClosingGU" type="boolean" truevalue="--noClosingGU" falsevalue="" checked="false" label="No GU pairing at the ends" help="don't allow pairing of G and U at the ends of helices."/>
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47 <param argument="--noTetra" type="boolean" truevalue="--noTetra" falsevalue="" checked="false" label="No stabilization for loops, hairpins etc." help=""/>
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48 <param argument="--nsp" type="text" value="" label="Allow other pairs in addition to the usual AU,GC,and GU pairs."
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49 help="Its argument is a comma separated list of additionally allowed pairs. If the first character is '-' then AB will imply that AB and BA are allowed pairs. e.g. RNAfold -nsp -GA will allow GA and AG pairs. Nonstandard pairs are given 0 stacking energy."/>
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50 </when>
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51 <when value="basic">
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52 </when>
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53 </conditional>
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54 </inputs>
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55 <outputs>
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56 <data format="txt" name="tabularFile"/>
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57 </outputs>
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58 <tests>
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59 <test>
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60 <param name="input" value="rnaduplex_input1.fa"/>
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61 <output name="out_file" file="rnaduplex_result1.txt"/>
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62 </test>
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63 </tests>
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64 <help>
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65 <![CDATA[
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66 **RNAduplex**
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67 RNA duplex reads two RNA sequences and computes optimal and suboptimal secondary structures for their hybridization. The calculation is simplified by allowing only inter−molecular base pairs, for the general case use RNAcofold. The computed optimal and suboptimal structure are written to stdout, one structure per line. Suboptimal structures are computed with energy in a certain range of the optimum (kcal/mol). Default is calculation of mfe structure only.
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68
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69 -----
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70
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71 **Input format**
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72
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73 RNAduplex requires one input file in Fasta format. The secondary strucures will be calculated pairwise: 1st sequence with 2nd sequence, 3rd sequence with 4th sequence, etc.
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74
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75 ------
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76
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77 **Outputs**
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79 Each line consist of: The structure in dot bracket format with a "&" separating the two strands. The range of the structure in the two sequences in the format "from,to : from,to"; the energy of duplex structure in kcal/mol. The format is especially useful for computing the hybrid structure between a small probe sequence and a long target sequence.
278760bf4bb2 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/vienna_rna commit d1bef7f37f272e3173c137fcb690364fee7e6d1f
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80
278760bf4bb2 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/vienna_rna commit d1bef7f37f272e3173c137fcb690364fee7e6d1f
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81
278760bf4bb2 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/vienna_rna commit d1bef7f37f272e3173c137fcb690364fee7e6d1f
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82 ]]>
278760bf4bb2 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/vienna_rna commit d1bef7f37f272e3173c137fcb690364fee7e6d1f
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83 </help>
278760bf4bb2 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/vienna_rna commit d1bef7f37f272e3173c137fcb690364fee7e6d1f
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84 <expand macro="citations" />
278760bf4bb2 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/vienna_rna commit d1bef7f37f272e3173c137fcb690364fee7e6d1f
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85 </tool>