--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/Build_tissue-specific_expression_dataset.R	Thu Dec 13 03:59:41 2018 -0500
@@ -0,0 +1,76 @@
+
+
+select_HPAimmunohisto<-function(hpa_ref, tissue, level, reliability) {
+  HPA.normal = read.table(hpa_ref,header=TRUE,sep="\t",stringsAsFactors = FALSE)
+  if (tissue == "tissue") {
+    tissue <- unique(HPA.normal$Tissue) 
+  }
+  if (level == "level") {
+    level <- unique(HPA.normal$Level)
+  }
+  if (reliability == "reliability") {
+    reliability <- unique(HPA.normal$Reliability)
+  }
+  res.imm <- subset(HPA.normal, Tissue%in%tissue & Level%in%level & Reliability%in%reliability)
+  return(res.imm)
+}
+
+    
+    
+select_HPARNAseq<-function(hpa_ref, sample) {
+  HPA.rnaTissue = read.table(hpa_ref,header=TRUE,sep="\t",stringsAsFactors = FALSE)
+  res.rna <- subset(HPA.rnaTissue, Sample%in%sample, select = -c(Unit))
+  colnames(res.rna)[which(colnames(res.rna) == 'Value')] <- 'Value (TPM unit)'
+  return(res.rna)
+}
+
+main <- function() {
+  args <- commandArgs(TRUE)
+  if(length(args)<1) {
+    args <- c("--help")
+  }
+  
+  # Help section
+  if("--help" %in% args) {
+    cat("Selection and Annotation HPA
+    Arguments:
+        --data_source: immuno/rnaseq
+        --hpe_ref: path to reference file normal_tissue.tsv/rna_tissue.tsv)
+          if immuno:
+            --tissue: list of tissues
+            --level: Not detected, Low, Medium, High
+            --reliability: Supported, Approved, Enhanced, Uncertain
+          if rnaseq:
+            --sample: Sample tissues 
+        --output: output filename \n")
+    q(save="no")
+  }
+  
+  # Parse arguments
+  parseArgs <- function(x) strsplit(sub("^--", "", x), "=")
+  argsDF <- as.data.frame(do.call("rbind", parseArgs(args)))
+  args <- as.list(as.character(argsDF$V2))
+  names(args) <- argsDF$V1
+
+  # Extract options
+  data_source = args$data_source
+  hpa_ref = args$hpa_ref
+  if (data_source == "immuno") {
+    tissue = strsplit(args$tissue, ",")[[1]]
+    level = strsplit(args$level, ",")[[1]]
+    reliability = strsplit(args$reliability, ",")[[1]]
+    # Calculation
+    res = select_HPAimmunohisto(hpa_ref, tissue, level, reliability)
+  }
+  else if (data_source == "rnaseq") {
+    sample = strsplit(args$sample, ",")[[1]]
+    # Calculation
+    res = select_HPARNAseq(hpa_ref, sample)
+  }
+
+  # Write output
+  output = args$output
+  write.table(res, output, sep = "\t", quote = FALSE, row.names = FALSE)
+}
+
+main()