Mercurial > repos > proteore > proteore_retrieve_from_hpa
annotate Build_tissue-specific_expression_dataset.xml @ 1:cfcc7a780991 draft default tip
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| author | proteore |
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| date | Thu, 13 Dec 2018 03:59:41 -0500 |
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1 <tool id="retrieve_from_hpa" name="Build tissue-specific expression dataset" version="2018.12.12"> |
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2 <description>[Human Protein Atlas](no input required)</description> |
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3 <requirements> |
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4 <requirement type="package" version="3.4.1">R</requirement> |
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5 </requirements> |
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6 <stdio> |
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7 <exit_code range="1:" /> |
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8 </stdio> |
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9 <command interpreter="Rscript"> |
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10 $__tool_directory__/Build_tissue-specific_expression_dataset.R |
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11 --data_source="$input.data_source" |
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12 #if $input.data_source == "immuno" |
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13 --hpa_ref="$__tool_directory__/normal_tissue.tsv" |
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14 --tissue="$input.normal_tissue" |
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15 --level="$input.level" |
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16 --reliability="$input.reliability" |
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17 #else if $input.data_source == "rnaseq" |
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18 --hpa_ref="$__tool_directory__/rna_tissue.tsv" |
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19 --sample="$input.sample" |
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20 #end if |
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21 --output="$output" |
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22 </command> |
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23 |
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24 <inputs> |
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25 <conditional name="input"> |
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26 <param name="data_source" type="select" label="Please choose experimental data source (antibody- or RNAseq-based)"> |
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27 <option value="immuno">Expression profiles based on immunohistochemistry</option> |
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28 <option value="rnaseq">RNA levels based on RNA-seq</option> |
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29 </param> |
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30 <when value="immuno"> |
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31 <param name="normal_tissue" type="select" label="Select tissue by clicking the dropdown menu below" multiple="True" optional="False"> |
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32 <option value="adrenal gland" >Adrenal gland</option> |
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33 <option value="appendix" >Appendix</option> |
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34 <option value="bone marrow" >Bone marrow</option> |
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35 <option value="breast" >Breast</option> |
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36 <option value="bronchus" >Bronchus</option> |
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37 <option value="caudate" >Caudate</option> |
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38 <option value="cerebellum" >Cerebellum</option> |
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39 <option value="cerebral cortex" >Cerebral cortex</option> |
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40 <option value="cervix" >Cervix</option> |
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41 <option value="colon" >Colon</option> |
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42 <option value="duodenum" >Duodenum</option> |
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43 <option value="endometrium 1" >Endometrium 1</option> |
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44 <option value="endometrium 2" >Endometrium 2</option> |
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45 <option value="epididymis" >Epididymis</option> |
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46 <option value="esophagus" >Esophagus</option> |
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47 <option value="fallopian tube" >Fallopian tube</option> |
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48 <option value="gallbladder" >Gallbladder</option> |
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49 <option value="heart muscle" >Heart muscle</option> |
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50 <option value="hippocampus" >Hippocampus</option> |
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51 <option value="kidney" >Kidney</option> |
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52 <option value="liver" >Liver</option> |
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53 <option value="lung" >Lung</option> |
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54 <option value="lymph node" >Lymph node</option> |
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55 <option value="nasopharynx" >Nasopharynx</option> |
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56 <option value="oral mucosa" >Oral mucosa</option> |
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57 <option value="ovary" >Ovary</option> |
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58 <option value="pancreas" >Pancreas</option> |
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59 <option value="parathyroid gland" >Parathyroid gland</option> |
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60 <option value="placenta" >Placenta</option> |
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61 <option value="prostate" >Prostate</option> |
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62 <option value="rectum" >Rectum</option> |
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63 <option value="salivary gland" >Salivary gland</option> |
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64 <option value="seminal vesicle" >Seminal vesicle</option> |
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65 <option value="skeletal muscle" >Skeletal muscle</option> |
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66 <option value="skin 1" >Skin 1</option> |
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67 <option value="skin 2" >Skin 2</option> |
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68 <option value="small intestine" >Small intestine</option> |
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69 <option value="smooth muscle" >Smooth muscle</option> |
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70 <option value="soft tissue 1" >Soft tissue 1</option> |
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71 <option value="soft tissue 2" >Soft tissue 2</option> |
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72 <option value="spleen" >Spleen</option> |
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73 <option value="stomach 1" >Stomach 1</option> |
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74 <option value="stomach 2" >Stomach 2</option> |
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75 <option value="testis" >Testis</option> |
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76 <option value="thyroid gland" >Thyroid gland</option> |
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77 <option value="tonsil" >Tonsil</option> |
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78 <option value="urinary bladder" >Urinary bladder</option> |
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79 <option value="vagina" >Vagina</option> |
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80 </param> |
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81 <param name="level" type="select" label="Expression level" display="checkboxes" multiple="True" optional="False"> |
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82 <option value="High" selected="true">High</option> |
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83 <option value="Medium">Medium</option> |
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84 <option value="Low">Low</option> |
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85 <option value="Not detected">Not detected</option> |
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86 </param> |
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87 <param name="reliability" type="select" label="Reliability score" display="checkboxes" multiple="True" optional="False"> |
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88 <option value="Enhanced" selected="true">Enhanced</option> |
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89 <option value="Supported" selected="true">Supported</option> |
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90 <option value="Approved">Approved</option> |
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91 <option value="Uncertain">Uncertain</option> |
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92 |
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93 </param> |
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94 </when> |
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95 <when value="rnaseq"> |
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96 <param name="sample" type="select" label="Keep and annotate genes present in the following tissue(s)" multiple="True" optional="False"> |
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97 <option value="adipose tissue">Adipose tissue</option> |
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98 <option value="adrenal gland">Adrenal gland</option> |
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99 <option value="appendix">Appendix</option> |
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100 <option value="bone marrow">Bone marrow</option> |
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101 <option value="breast">Breast</option> |
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102 <option value="cerebral cortex">Cerebral cortex</option> |
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103 <option value="cervix, uterine">Cervix, uterine</option> |
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104 <option value="colon">Colon</option> |
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105 <option value="duodenum">Duodenum</option> |
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106 <option value="endometrium">Endometrium</option> |
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107 <option value="epididymis">Epididymis</option> |
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108 <option value="esophagus">Esophagus</option> |
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109 <option value="fallopian tube">Fallopian tube</option> |
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110 <option value="gallbladder">Gallbladder</option> |
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111 <option value="heart muscle">Heart muscle</option> |
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112 <option value="kidney">Kidney</option> |
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113 <option value="liver">Liver</option> |
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114 <option value="lung">Lung</option> |
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115 <option value="lymph node">Lymph node</option> |
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116 <option value="ovary">Ovary</option> |
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117 <option value="pancreas">Pancreas</option> |
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118 <option value="parathyroid gland">Parathyroid gland</option> |
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119 <option value="placenta">Placenta</option> |
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120 <option value="prostate">Prostate</option> |
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121 <option value="rectum">Rectum</option> |
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122 <option value="salivary gland">Salivary gland</option> |
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123 <option value="seminal vesicle">Seminal vesicle</option> |
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124 <option value="skeletal muscle">Skeletal muscle</option> |
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125 <option value="skin">Skin</option> |
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126 <option value="small intestine">Small intestine</option> |
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127 <option value="smooth muscle">Smooth muscle</option> |
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128 <option value="spleen">Spleen</option> |
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129 <option value="stomach">Stomach</option> |
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130 <option value="testis">Testis</option> |
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131 <option value="thyroid gland">Thyroid gland</option> |
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132 <option value="tonsil">Tonsil</option> |
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133 <option value="rinary bladder">Urinary bladder</option> |
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134 </param> |
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135 </when> |
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136 </conditional> |
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137 </inputs> |
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138 |
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139 <outputs> |
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140 <data name="output" format="tsv" label=""/> |
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141 </outputs> |
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142 |
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143 <tests> |
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144 <test> |
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145 <conditional name="input"> |
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146 <param name="data_source" value="immuno"/> |
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147 <param name="normal_tissue" value="lung,ovary"/> |
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148 <param name="level" value="High"/> |
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149 <param name="reliability" value="Approved,Supported"/> |
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150 </conditional> |
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151 <output name="output" file="test.txt"/> |
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152 </test> |
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153 </tests> |
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154 |
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155 <help><![CDATA[ |
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156 |
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157 This tool allows to retrieve information from Human Protein Atlas (https://www.proteinatlas.org/) |
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158 regarding the expression profiles of human genes both at the mRNA and protein levels |
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159 without any input required. It could be used to: |
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160 |
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161 * Select a list of proteins highly expressed in a given tissue for data mining or comparison purpose in terms of expected protein content from one tissue to another |
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162 |
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163 * Build a "reference" proteome restricted to a given tissue (for instance for GO analysis as a reference background restricted to a biological sample) |
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164 |
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165 The resources from Human Protein Atlas that can be queried are the following: |
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166 |
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167 1. Human normal tissue data: expression profiles for proteins in human tissues based on immunohistochemisty using tissue micro arrays measured in 58 tissues and 82 cell types. |
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168 |
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169 The tab-separated file includes Ensembl gene identifier ("Gene"), tissue name ("Tissue"), annotated cell type ("Cell type"), expression value ("Level"), and the gene reliability of the expression value ("Reliability score"). |
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170 |
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171 The reliability score is divided into Enhanced, Supported, Approved, or Uncertain with the following definitions: |
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172 |
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173 * Enhanced: One or several antibodies with non-overlapping epitopes targeting the same gene have obtained enhanced validation based on orthogonal or independent antibody validation method. |
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174 |
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175 * Supported: Consistency with RNA-seq and/or protein/gene characterization data, in combination with similar staining pattern if independent antibodies are available. |
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176 |
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177 * Approved: Consistency with RNA-seq data in combination with inconsistency with, or lack of, protein/gene characterization data. Alternatively, consistency with protein/gene characterization data in combination with inconsistency with RNA-seq data. If independent antibodies are available, the staining pattern is partly similar or dissimilar. |
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178 |
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179 * Uncertain: |
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180 |
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181 Inconsistency with, or lack of, RNA-seq and/or protein/gene characterization data, in combination with dissimilar staining pattern if independent antibodies are available. |
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182 |
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183 The data is based on The Human Protein Atlas version 18 and Ensembl version 88.38. |
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184 |
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185 2. RNA levels based on RNA-seq data: RNA levels measured in 64 cell lines and 37 tissues based on RNA-seq experiments. The tab-separated file includes Ensembl gene identifier ("Gene"), analysed sample ("Sample") and transcripts per million ("Value" and "Unit"). |
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186 |
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187 The data is based on The Human Protein Atlas version 18 and Ensembl version 88.38. |
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188 |
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189 For more information: https://www.proteinatlas.org/about/help |
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190 |
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191 ----- |
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192 |
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193 .. class:: infomark |
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194 |
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195 **Authors** |
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196 |
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197 T.P. Lien Nguyen, Florence Combes, Yves Vandenbrouck CEA, INSERM, CNRS, Grenoble-Alpes University, BIG Institute, FR |
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198 |
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199 Sandra Dérozier, Olivier Rué, Christophe Caron, Valentin Loux INRA, Paris-Saclay University, MAIAGE Unit, Migale Bioinformatics platform |
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200 |
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201 This work has been partially funded through the French National Agency for Research (ANR) IFB project. |
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202 |
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203 Contact support@proteore.org for any questions or concerns about the Galaxy implementation of this tool. |
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204 |
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205 ]]></help> |
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206 |
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207 <citations> |
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208 </citations> |
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209 |
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210 </tool> |
