diff pal_finder_wrapper.xml @ 9:b3ca3ece8a8b draft

WIP detect/trap "bad primer product range" errors.
author pjbriggs
date Fri, 16 Jun 2017 09:12:09 -0400
parents 67ab365c29a7
children 84613d5f1713
line wrap: on
line diff
--- a/pal_finder_wrapper.xml	Tue Jun 06 05:56:12 2017 -0400
+++ b/pal_finder_wrapper.xml	Fri Jun 16 09:12:09 2017 -0400
@@ -274,6 +274,29 @@
 
 -------------
 
+.. class:: warning
+
+**Known problems**
+
+.. class:: infomark
+
+**Bad primer product size ranges**
+
+For some datasets pal_finder may generate 'bad' product size ranges (where the
+lower limit exceeds the upper limit) for one or more reads, for input into
+primer3_core.
+
+If this occurs then the tool will terminate with an error. A list of the reads
+for which the bad ranges were generated can be found in the error message
+which can be accessed via the 'bug' icon from a failed dataset.
+
+The conditions which cause this error are unclear. However we believe it to be
+associated with short or low quality reads. It is recommended that the input
+data are sufficiently trimmed and filtered (using e.g. the Trimmomatic tool)
+before rerunning pal_finder.
+
+-------------
+
 .. class:: infomark
 
 **Credits**