Mercurial > repos > pjbriggs > pal_finder
diff pal_finder_wrapper.xml @ 9:b3ca3ece8a8b draft
WIP detect/trap "bad primer product range" errors.
author | pjbriggs |
---|---|
date | Fri, 16 Jun 2017 09:12:09 -0400 |
parents | 67ab365c29a7 |
children | 84613d5f1713 |
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--- a/pal_finder_wrapper.xml Tue Jun 06 05:56:12 2017 -0400 +++ b/pal_finder_wrapper.xml Fri Jun 16 09:12:09 2017 -0400 @@ -274,6 +274,29 @@ ------------- +.. class:: warning + +**Known problems** + +.. class:: infomark + +**Bad primer product size ranges** + +For some datasets pal_finder may generate 'bad' product size ranges (where the +lower limit exceeds the upper limit) for one or more reads, for input into +primer3_core. + +If this occurs then the tool will terminate with an error. A list of the reads +for which the bad ranges were generated can be found in the error message +which can be accessed via the 'bug' icon from a failed dataset. + +The conditions which cause this error are unclear. However we believe it to be +associated with short or low quality reads. It is recommended that the input +data are sufficiently trimmed and filtered (using e.g. the Trimmomatic tool) +before rerunning pal_finder. + +------------- + .. class:: infomark **Credits**