Mercurial > repos > peterjc > tmhmm_and_signalp
diff tools/protein_analysis/rxlr_motifs.xml @ 20:a538e182fab3 draft
Uploaded v0.2.5 preview 4, adding Cock et al. 2003 citation information.
author | peterjc |
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date | Tue, 10 Sep 2013 08:55:19 -0400 |
parents | 2b35b5c4b7f4 |
children | 90e3d02f8013 |
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--- a/tools/protein_analysis/rxlr_motifs.xml Thu May 23 12:49:18 2013 -0400 +++ b/tools/protein_analysis/rxlr_motifs.xml Tue Sep 10 08:55:19 2013 -0400 @@ -74,9 +74,9 @@ Looks for the oomycete motif RXLR as described in Bhattacharjee et al. (2006). Matches must have a SignalP Hidden Markov Model (HMM) score of at least 0.9, -a SignalP Neural Network (NN) predicted clevage site giving a signal peptide +a SignalP Neural Network (NN) predicted cleavage site giving a signal peptide length between 10 and 40 amino acids inclusive, and the RXLR pattern must be -after but within 100 amino acids of the clevage site. +after but within 100 amino acids of the cleavage site. SignalP is run truncating the sequences to the first 70 amino acids, which was the default on the SignalP webservice used in Bhattacharjee et al. (2006). @@ -86,9 +86,9 @@ Looks for the protein motif RXLR as described in Win et al. (2007). Matches must have a SignalP Hidden Markov Model (HMM) score of at least 0.9, -a SignalP Neural Network (NN) predicted clevage site giving a signal peptide +a SignalP Neural Network (NN) predicted cleavage site giving a signal peptide length between 10 and 40 amino acids inclusive, and the RXLR pattern must be -after the clevage site and start between amino acids 30 and 60. +after the cleavage site and start between amino acids 30 and 60. SignalP is run truncating the sequences to the first 70 amino acids, to match the methodology of Torto et al. (2003) followed in Win et al. (2007). @@ -135,6 +135,14 @@ **References** +If you use this Galaxy tool in work leading to a scientific publication please +cite Cock et al. (2013) and the appropriate method paper(s): + +Peter Cock, Bjoern Gruening, Konrad Paszkiewicz and Leighton Pritchard (2013). +Galaxy tools and workflows for sequence analysis with applications +in molecular plant pathology. PeerJ 1:e167 +http://dx.doi.org/10.7717/peerj.167 + Stephen C. Whisson, Petra C. Boevink, Lucy Moleleki, Anna O. Avrova, Juan G. Morales, Eleanor M. Gilroy, Miles R. Armstrong, Severine Grouffaud, Pieter van West, Sean Chapman, Ingo Hein, Ian K. Toth, Leighton Pritchard and Paul R. J. Birch A translocation signal for delivery of oomycete effector proteins into host plant cells. Nature 450:115-118, 2007.